Ion exchange type extraction device for purifying lysozyme in egg white

Abstract

The invention belongs to the technical field of extraction devices, and particularly relates to an ion exchange type extraction device for purifying lysozyme in egg white, the ion exchange type extraction device comprises a stirring cylinder, the upper side wall of the stirring cylinder is fixedly connected with a PLC controller, the front side wall of the stirring cylinder is fixedly connected with an operation panel, and the outer wall of the stirring cylinder is fixedly connected with a support ring. In the stirring process of raw materials, the raw materials in the central area and the peripheral area can be automatically sucked in a reciprocating mode, so that the stirring speed of the raw materials in the device is increased, lysozyme is evenly distributed in the whole system, and in the stirring process of the raw materials through the spiral stirring paddle, the lysozyme is evenly distributed in the whole system. When the spiral stirring paddle generates more heat due to friction with liquid, the spiral stirring paddle can be automatically cooled, so that the problem that the lysozyme activity and the extraction efficiency are reduced due to over-high heat on the surface of the spiral stirring paddle is avoided.

Description

Technical Field

[0001] The invention belongs to the technical field of extraction devices, and in particular relates to an ion exchange extraction device for purifying lysozyme from egg white. Background Art

[0002] Lysozyme, as an enzyme with unique biological activity, is widely used in many fields. Purifying it from egg white is of great significance. Activated ion exchange resin and diluted and pH-adjusted egg white solution are added to a stirring tank. Lysozyme is fully contacted with the resin by stirring, and ion exchange occurs and the lysozyme is adsorbed. For example, an extraction device for purifying lysozyme from egg white is proposed in patent announcement No. CN220643063U.

[0003] When the existing stirring device stirs the raw materials, the central area is directly subjected to the high-speed stirring of the stirring blades and is subjected to a strong shear force. This high-intensity shear force can destroy some interactions between protein molecules, disrupt the originally possible ordered structure, and promote the protein molecules to be more dispersed in the solution, thereby causing the egg white viscosity in the central area to decrease. In contrast, the peripheral area is far away from the stirring blades and is subjected to weaker shear force, so the interaction between protein molecules is better maintained, resulting in a higher viscosity of the egg white in the peripheral area. Due to the difference in viscosity between the central and peripheral areas, the diffusion speed of lysozyme in these two areas is also different. In the central area with lower viscosity, lysozyme molecules can diffuse more easily with the movement of the fluid and are distributed more evenly; while in the peripheral area with higher viscosity, the diffusion of lysozyme molecules is hindered, and local concentrations are prone to be too high or too low, which ultimately leads to uneven distribution of lysozyme in the entire system.

[0004] Therefore, an ion exchange extraction device for purifying lysozyme from egg white is proposed to solve the above problems. Summary of the invention

[0005] The object of the present invention is to provide an ion exchange extraction device for purifying lysozyme from egg white in view of the above problems.

[0006] To achieve the above object, the present invention adopts the following technical scheme: an ion exchange extraction device for purifying lysozyme from egg white, comprising a mixing drum, the upper side wall of the mixing drum is fixedly connected to a PLC controller, the front side wall of the mixing drum is fixedly connected to an operation panel, the outer wall of the mixing drum is fixedly connected to a support ring, the lower side wall of the support ring is fixedly connected to a support frame, the upper side wall of the mixing drum is fixedly connected to a feed pipe, the lower side wall of the mixing drum is fixedly connected to a discharge pipe, a control valve is provided in the discharge pipe, and further comprising: A stirring shaft is rotatably connected to the upper side wall of the stirring drum, a stirring motor is fixedly connected to the upper side wall of the stirring drum, the stirring motor is transmission-connected to the stirring shaft through a bevel gear transmission assembly, and a spiral stirring paddle is fixedly connected to the rod wall of the stirring shaft through a bracket; The reflux assembly is arranged on the inner wall of the mixing drum and is used for mixing raw materials at different positions; The cooling assembly is arranged on the outer wall of the stirring shaft and is used to reduce the heat generated by the spiral stirring paddle during the stirring process.

[0007] Preferably, the reflux assembly includes a plurality of reflux pipes arranged on the inner wall of the mixing drum, the reflux pipe is an annular structure, the inner wall of the reflux pipe is fixedly connected with a plurality of reflux heads, the right side wall of the mixing drum is fixedly connected with a mounting frame, the inner wall of the mounting frame is fixedly connected with a first pump, the feed end of the first pump is fixedly connected with a suction pipe, the pipe wall of the suction pipe is fixedly connected with a plurality of transverse pipes, one end of the transverse pipe away from the suction pipe passes through the mixing drum and is connected with the reflux pipe, a first regulating valve is arranged in the transverse pipe, and the discharge end of the first pump is fixedly connected with a discharge pipe, The inner wall of the mixing drum is connected with a discharge pipe, which is an annular structure. The inner wall of the discharge pipe is fixedly connected with a plurality of discharge heads. The upper end of the discharge pipe passes through the mixing drum and is connected with the discharge pipe. The upper side wall of the mixing drum is fixedly connected with a second pump through a bracket. The mixing shaft is a hollow structure. The rod wall of the mixing shaft is connected with a plurality of liquid inlet heads. The liquid inlet pipe of the second pump is rotatably connected with the inner wall of the mixing shaft through a sealed bearing. The discharge end of the second pump is connected with the discharge pipe. The liquid inlet pipe and the pipe wall of the suction pipe of the second pump are both connected with detection components.

[0008] Preferably, the detection component includes a mesh plate arranged on the inner walls of the suction pipe and the liquid inlet pipe, the inner walls of the suction pipe and the liquid inlet pipe are connected to the mesh plate by elastic rods, the outer walls of the suction pipe and the liquid inlet pipe are connected to a detection box by a bracket, the inner walls of the two detection boxes are connected to pressure sensors, the detection ends of the pressure sensors are connected to a piston plate, the upper side walls of the two detection boxes are fixedly connected to detection tubes, the ends of the two detection tubes away from the detection box respectively pass through the suction pipe and the liquid inlet pipe, and are connected to a compression tube, the end of the compression tube away from the detection tube is connected to the mesh plate, the pressure sensor is electrically connected to the PLC controller, and the upper side wall of the mixing drum is fixedly connected to an analysis component.

[0009] Preferably, the analysis component includes two analysis boxes arranged above the mixing drum, the two analysis boxes are connected by a bracket, the right inner walls of the two analysis boxes are connected to a movable plate by a spring, the right inner walls of the two analysis boxes are fixedly connected to an electromagnetic block, the right wall of the movable plate is fixedly connected to a permanent magnet block, the side walls on the opposite sides of the two movable plates are fixedly connected to analysis rods, the opposite ends of the two analysis rods pass through the analysis box, the side walls of the analysis box are provided with sliding openings that match the analysis rods, the upper ends of the analysis rods on the lower side are fixedly connected to a conductive seat, the conductive seat is electrically connected to an external power supply, the lower end of the analysis rods is fixedly connected to a connecting frame, the inner wall of the connecting frame is fixedly connected to two conductive plates, and the two conductive plates are electrically connected to a PLC controller.

[0010] Preferably, the cooling assembly includes an annular water tank fixedly mounted on the outer wall of the stirring shaft, a water pump is fixedly connected to the upper side wall of the annular water tank, the liquid inlet end of the water pump is located in the annular water tank, the liquid outlet end of the water pump is fixedly connected to a water supply pipe, two connecting pipes are fixedly connected between the stirring shaft and the spiral stirring paddle, and the spiral stirring paddle is a hollow structure, the lower end of the water supply pipe is connected to the upper water supply pipe, and the end of the connecting pipe located at the bottom close to the stirring shaft is connected to a return pipe, and the upper end of the return pipe is connected to the annular water tank.

[0011] Preferably, a protective cover is fixedly connected to the upper side wall of the mixing drum, and the second pump and the mixing motor are both located inside the protective cover.

[0012] Preferably, a plurality of semiconductor refrigeration plates are plugged into the upper side wall of the annular water tank, and the heat release ends of the plurality of semiconductor refrigeration plates all extend out of the annular water tank.

[0013] Preferably, a temperature sensor is provided on the surface of the spiral stirring paddle, and the temperature sensor is electrically connected to a PLC controller.

[0014] Compared with the existing technology, the advantages of an ion exchange extraction device for purifying lysozyme from egg white are: By setting up the reflux component, detection component and analysis component, the raw materials in the central area and the peripheral area can be automatically pumped back and forth during the stirring of the raw materials, thereby increasing the stirring speed of the raw materials inside the device and making the lysozyme evenly distributed in the entire system.

[0015] By setting up a cooling component, when the spiral stirring paddle stirs the raw materials and generates more heat due to friction with the liquid, the spiral stirring paddle can be automatically cooled down to avoid the problem of excessive heat on the surface of the spiral stirring paddle reducing the activity of lysozyme and extraction efficiency.

[0016] By setting up the reflux component, the stirring condition of the raw materials inside the device can be detected in real time during the process of stirring the raw materials by the stirring device. When it is detected that the stirring of the raw materials inside the device is completed, the device can be controlled to stop working in time, which can avoid the device working too long and causing energy waste, while also ensuring the purification quality of lysozyme. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic structural diagram of an ion exchange extraction device for purifying lysozyme from egg white provided by the present invention; Figure 2 The invention provides an ion exchange extraction device for purifying lysozyme from egg white. Figure 1 A partial cross-sectional view of Figure 3 It is a schematic diagram of the internal structure of a mounting frame in an ion exchange extraction device for purifying lysozyme from egg white provided by the present invention; Figure 4 It is a schematic structural diagram of a detection component in an ion exchange extraction device for purifying lysozyme from egg white provided by the present invention; Figure 5 It is a schematic structural diagram of an analysis component in an ion exchange extraction device for purifying lysozyme from egg white provided by the present invention; Figure 6 It is a schematic diagram of the positional relationship between the conductive seat and the connecting frame in an ion exchange extraction device for purifying lysozyme from egg white provided by the present invention; Figure 7 It is a schematic structural diagram of a cooling component in an ion exchange extraction device for purifying lysozyme from egg white provided by the present invention; Figure 8 The present invention provides a schematic diagram of the internal structure of a stirring shaft in an ion exchange extraction device for purifying lysozyme from egg white.

[0018] In the figure: 1 mixing drum, 2 PLC controller, 3 operation panel, 4 support ring, 5 support frame, 6 feed pipe, 7 discharge pipe, 8 control valve, 9 mixing shaft, 10 mixing motor, 11 spiral stirring paddle, 12 reflux assembly, 121 reflux pipe, 122 reflux head, 13 mounting frame, 14 first pump, 15 suction pipe, 16 transverse pipe, 17 first regulating valve, 18 discharge pipe, 19 discharge pipe, 20 discharge head, 21 second pump, 22 feed head, 23 feed pipe, 24 detection Component, 241 mesh plate, 242 detection box, 25 pressure sensor, 26 piston plate, 27 detection tube, 28 compression tube, 29 analysis component, 291 analysis box, 292 moving plate, 30 electromagnetic block, 31 permanent magnet block, 32 analysis rod, 33 conductive seat, 34 connecting frame, 35 conductive plate, 36 cooling component, 361 annular water tank, 362 water pump, 37 water supply pipe, 38 connecting pipe, 39 return pipe, 40 protective cover, 41 semiconductor refrigeration plate, 42 temperature sensor. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0020] like Figure 1-Figure 8 As shown, an ion exchange extraction device for purifying lysozyme from egg white comprises a mixing drum 1, a PLC controller 2 is fixedly connected to the upper side wall of the mixing drum 1, an operation panel 3 is fixedly connected to the front side wall of the mixing drum 1, a support ring 4 is fixedly connected to the outer wall of the mixing drum 1, a support frame 5 is fixedly connected to the lower side wall of the support ring 4, a feed pipe 6 is fixedly connected to the upper side wall of the mixing drum 1, a discharge pipe 7 is fixedly connected to the lower side wall of the mixing drum 1, a control valve 8 is arranged in the discharge pipe 7, and the device further comprises: A stirring shaft 9 is rotatably connected to the upper side wall of the stirring drum 1, a stirring motor 10 is fixedly connected to the upper side wall of the stirring drum 1, the stirring motor 10 is transmission-connected to the stirring shaft 9 through a bevel gear transmission assembly, and a spiral stirring paddle 11 is fixedly connected to the rod wall of the stirring shaft 9 through a bracket; The reflux assembly 12 is arranged on the inner wall of the mixing drum 1 and is used for mixing raw materials at different positions; The cooling assembly 36 is disposed on the outer wall of the stirring shaft 9 and is used to reduce the heat generated by the spiral stirring blade 11 during the stirring process.

[0021] The reflux assembly 12 includes a plurality of reflux pipes 121 arranged on the inner wall of the mixing drum 1. The reflux pipe 121 is an annular structure. The inner wall of the reflux pipe 121 is fixedly connected with a plurality of reflux heads 122. The right side wall of the mixing drum 1 is fixedly connected with a mounting frame 13. The inner wall of the mounting frame 13 is fixedly connected with a first pump 14. The feed end of the first pump 14 is fixedly connected with a suction pipe 15. The pipe wall of the suction pipe 15 is fixedly connected with a plurality of transverse pipes 16. The end of the transverse pipe 16 away from the suction pipe 15 passes through the mixing drum 1 and is connected with the reflux pipe 121. A first regulating valve 17 is arranged in the transverse pipe 16. The discharge end of the first pump 14 is fixedly connected with a discharge pipe 18. The inner wall of the mixing drum 1 is connected with a discharge pipe 19. The discharge pipe 19 is an annular structure. The inner wall is fixedly connected with a plurality of liquid discharge heads 20, the upper end of the discharge pipe 18 passes through the mixing drum 1 and is connected with the liquid discharge pipe 19, the upper side wall of the mixing drum 1 is fixedly connected with a second pump 21 through a bracket, the mixing shaft 9 is a hollow structure, the rod wall of the mixing shaft 9 is connected with a plurality of liquid inlet heads 22, the liquid inlet pipe 23 of the second pump 21 is rotatably connected to the inner wall of the mixing shaft 9 through a sealed bearing, the discharge end of the second pump 21 is connected with the liquid discharge pipe 19, the liquid inlet pipe 23 of the second pump 21 and the tube wall of the suction pipe 15 are both connected with a detection component 24, in the process of stirring the raw materials by using the stirring device, the stirring condition of the raw materials inside the device can be detected in real time, while avoiding the device from working too long and reducing energy consumption, the purification quality of lysozyme is also guaranteed.

[0022] The detection component 24 includes a mesh plate 241 arranged on the inner walls of the suction pipe 15 and the liquid inlet pipe 23, the inner walls of the suction pipe 15 and the liquid inlet pipe 23 are connected to the mesh plate 241 through an elastic rod, the outer walls of the suction pipe 15 and the liquid inlet pipe 23 are connected to a detection box 242 through a bracket, the inner walls of the two detection boxes 242 are connected to a pressure sensor 25, the detection end of the pressure sensor 25 is connected to a piston plate 26, the upper side walls of the two detection boxes 242 are fixedly connected to a detection tube 27, the ends of the two detection tubes 27 away from the detection box 242 respectively pass through the suction pipe 15 and the liquid inlet pipe 23, and are connected to a compression tube 28, the end of the compression tube 28 away from the detection tube 27 is connected to the mesh plate 241, the pressure sensor 25 is electrically connected to the PLC controller 2, and the upper side wall of the mixing drum 1 is fixedly connected to an analysis component 29, which can detect the viscosity of raw materials at different positions in the mixing drum 1.

[0023] The analysis component 29 includes two analysis boxes 291 arranged above the mixing drum 1. The two analysis boxes 291 are connected by a bracket. The right inner walls of the two analysis boxes 291 are connected to a moving plate 292 through a spring. The right inner walls of the two analysis boxes 291 are fixedly connected to an electromagnetic block 30. The right wall of the moving plate 292 is fixedly connected to a permanent magnet block 31. The side walls of the two moving plates 292 on the opposite side are fixedly connected to analysis rods 32. The opposite ends of the two analysis rods 32 pass through the analysis box 291. The side wall of the analysis box 291 is provided with a sliding opening that matches the analysis rod 32. The upper end of the lower analysis rod 32 is fixedly connected to a conductive seat 33, and the conductive seat 33 is electrically connected to an external power supply. The lower end of the analysis rod 32 is fixedly connected to a connecting frame 34. The inner wall of the connecting frame 34 is fixedly connected to two conductive plates 35. The two conductive plates 35 are electrically connected to the PLC controller 2, so as to analyze the viscosity of the raw material.

[0024] The cooling assembly 36 includes an annular water tank 361 fixedly mounted on the outer wall of the stirring shaft 9, a water pump 362 is fixedly connected to the upper side wall of the annular water tank 361, a liquid inlet end of the water pump 362 is located in the annular water tank 361, and a liquid outlet end of the water pump 362 is fixedly connected to a water supply pipe 37, two connecting pipes 38 are fixedly connected between the stirring shaft 9 and the spiral stirring paddle 11, and the spiral stirring paddle 11 is a hollow structure, the lower end of the water supply pipe 37 is connected to the upper water supply pipe 37, and the end of the lower connecting pipe 38 close to the stirring shaft 9 is connected to a return pipe 39, and the upper end of the return pipe 39 is connected to the annular water tank 361. In the process of the spiral stirring paddle 11 stirring the internal raw materials, the heat generated by the friction between the spiral stirring paddle 11 and the liquid can be reduced, thereby avoiding the problem that excessive heat will reduce the activity of lysozyme and the extraction efficiency.

[0025] A protective cover 40 is fixedly connected to the upper side wall of the mixing drum 1 , and the second pump 21 and the mixing motor 10 are both located in the protective cover 40 , which can protect the second pump 21 and the mixing motor 10 .

[0026] A plurality of semiconductor refrigeration plates 41 are inserted into the upper side wall of the annular water tank 361 . The heat release ends of the plurality of semiconductor refrigeration plates 41 extend out of the annular water tank 361 , so as to cool down the cooling water inside the annular water tank 361 .

[0027] A temperature sensor 42 is provided on the surface of the propeller 11 . The temperature sensor 42 is electrically connected to the PLC controller 2 and can detect the surface temperature of the propeller 11 .

[0028] The operating principle of the present invention is now described as follows: the activated ion exchange resin and the diluted and pH-adjusted egg white solution are transported to the mixing drum 1 through the feed pipe 6, and then an electrical signal is transmitted to the PLC controller 2 through the operation panel 3. After receiving the electrical signal, the PLC controller 2 controls the stirring motor 10 to work. The stirring motor 10 is connected to the stirring shaft 9 through the bevel gear transmission assembly. The stirring motor 10 controls the stirring shaft 9 to rotate, and the stirring shaft 9 drives the spiral stirring paddle 11 to rotate. The raw materials in the mixing drum 1 are stirred by the spiral stirring paddle 11. When the PLC controller 2 controls the stirring motor 10 to work, the PLC controller 2 also The first pump 14 and the second pump 21 are controlled to work simultaneously. The first pump 14 delivers multiple raw materials near the side wall of the mixing drum 1 to the discharge pipe 19 through the suction pipe 15, the discharge pipe 18, the multiple reflux heads 122, the reflux pipe 121 and the cross pipe 16, and discharges them through the discharge head 20. At the same time, the second pump 21 delivers the raw materials near the mixing shaft 9 to the discharge pipe 19 through the mixing shaft 9, the inlet pipe 23 and the multiple inlet heads 22, and discharges them through the discharge head 20. While the raw materials are being stirred by the spiral stirring paddle 11, the raw materials at the inner periphery and the center of the mixing drum 1 can also be mixed, thereby further improving the mixing effect of the raw materials in the mixing drum 1. When the first pump 14 and the second pump 21 are working, the raw material will flow in the suction pipe 15 and the liquid inlet pipe 23. When the raw material passes through the mesh plate 241 in the suction pipe 15 and the liquid inlet pipe 23, the raw material will drive the mesh plate 241 to overcome the elastic force of the elastic rod and move. The greater the viscosity of the raw material, the greater the resistance the mesh plate 241 encounters when the raw material flows. When the mesh plate 241 moves, it will squeeze the compression tube 28. The compression tube 28 will transport the internal gas to the detection box 242 through the detection tube 27, so that the air pressure on one side of the piston plate 26 increases. The piston plate 26 will squeeze the pressure sensor 25, and the pressure sensor 25 will send a The greater the pressure on the pressure sensor 25, the greater the electric signal transmitted to the PLC controller 2. The PLC controller 2 will control the external current of different sizes to be transmitted to the electromagnetic block 30 according to the size of the electric signal transmitted by the pressure sensor 25. The pressure sensor 25 located on the outside of the suction pipe 15 will transmit the external current to the electromagnetic block 30 on the lower side through the PLC controller 2. The pressure sensor 25 located on the outside of the liquid inlet pipe 23 will transmit the external current to the electromagnetic block 30 on the upper side through the PLC controller 2. When the viscosity of the outer area and the central area of ​​the mixing drum 1 is different (the raw materials have not been mixed evenly), the current is transmitted to the two electromagnetic blocks 30. The current intensity on the circuit is different, and the two electromagnetic blocks 30 will generate different magnetic attraction forces. The two electromagnetic blocks 30 will attract the two permanent magnet blocks 31 and the moving plate 292 to move different distances. The two moving plates 292 respectively drive the conductive seat 33 and the conductive plate 35 to move different distances through the analysis rod 32, so that the conductive seat 33 and the conductive plate 35 are in contact, the conductive seat 33 is electrically connected to the external power supply, and the conductive plate 35 is electrically connected to the PLC controller 2. When the conductive seat 33 and the conductive plate 35 are in contact, the external current signal will be transmitted to the PLC controller 2. When the PLC controller 2 receives the electrical signal, it indicates that the raw materials inside the mixing drum 1 are not mixed evenly. The stirring work needs to be continued. Similarly, when the raw materials in the central area and the peripheral area of ​​the mixing drum 1 are evenly stirred, the two movable plates 292 drive the conductive seat 33 and the conductive plate 35 to move the same distance through the analysis rod 32, and the conductive seat 33 will be between the two conductive plates 35, and the conductive seat 33 will not contact the conductive plate 35. When the PLC controller 2 does not receive the electrical signal transmitted from the conductive plate 35 within five seconds, the PLC controller 2 will control the stirring motor 10, the first pump 14 and the second pump 21 to stop working. At the same time, the PLC controller 2 will also control the internal buzzer module to emit a prompt sound to remind the operator that the raw material mixing is completed; When the propeller 11 is working, there is a strong friction between it and the raw material. The propeller 11 pushes the liquid to flow at a certain speed and force, and the liquid generates a reaction force on the propeller 11. This friction will cause energy loss, and this lost energy will be converted into heat energy, causing the temperature of the side of the propeller 11 shearing the liquid to rise. When the temperature of the propeller 11 rises to a certain level, the activity of the lysozyme will be reduced or even inactivated, seriously affecting the purification effect. When the temperature sensor 42 on the surface of the propeller 11 detects the temperature rise, the temperature sensor 42 will control the water pump 362 and the semiconductor refrigeration plate 41 to work simultaneously through the PLC controller 2, the semiconductor refrigeration plate 41 will reduce the temperature of the coolant in the annular water tank 361, the water pump 362 will transport the coolant with reduced temperature to the upper connecting pipe 38 through the water supply pipe 37, and then transport it to the spiral stirring blade 11 (the spiral stirring blade 11 is a hollow structure) through the connecting pipe 38, so as to take away the heat generated by the spiral stirring blade 11, and the coolant will be transported to the return pipe 39 through the lower connecting pipe 38, and the coolant with increased temperature will be transported to the return pipe 39 through the return pipe 39. Refrigeration is performed in the annular water tank 361 to reduce the surface temperature of the spiral stirring blade 11, thereby avoiding the problem that excessive heat on the surface of the spiral stirring blade 11 will reduce the activity of lysozyme and the extraction efficiency. After the stirring is completed, the stirring is stopped and the system is allowed to stand for a period of time. Since the ion exchange resin usually has a certain particle size and density, during the standing process, the resin particles will gradually settle to the bottom of the container, and the substances not adsorbed by the resin, including some water, unadsorbed small molecular impurities, etc., will remain in the solution, located above the resin layer. This part of the solution is the supernatant, and the operator uses the feed pipe 6 to remove the supernatant. , insert the liquid inlet end of the external peristaltic pump into the supernatant, and extract the supernatant, retain the ion exchange resin adsorbed with lysozyme, add an eluent (such as a 0.5-2 mol / L sodium chloride solution) to the ion exchange resin adsorbed with lysozyme, so that the ionic bond between the lysozyme and the resin is destroyed, thereby eluting the lysozyme from the resin, and collecting the eluted eluent containing lysozyme through the discharge pipe 7. At this time, the lysozyme concentration in the eluent is relatively high. Subsequently, the eluent can be concentrated by ultrafiltration, dialysis or freeze-drying to further purify the lysozyme.

[0029] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An ion exchange extraction device for purifying lysozyme from egg white, comprising a mixing drum (1), the upper side wall of the mixing drum (1) being fixedly connected to a PLC controller (2), the front side wall of the mixing drum (1) being fixedly connected to an operation panel (3), the outer wall of the mixing drum (1) being fixedly connected to a support ring (4), the lower side wall of the support ring (4) being fixedly connected to a support frame (5), the upper side wall of the mixing drum (1) being fixedly connected to a feed pipe (6), the lower side wall of the mixing drum (1) being fixedly connected to a discharge pipe (7), the discharge pipe (7) being provided with a control valve (8), characterized in that: Also includes: A stirring shaft (9) is rotatably connected to the upper side wall of the stirring drum (1); a stirring motor (10) is fixedly connected to the upper side wall of the stirring drum (1); the stirring motor (10) is transmission-connected to the stirring shaft (9) via a bevel gear transmission assembly; a spiral stirring paddle (11) is fixedly connected to the rod wall of the stirring shaft (9) via a bracket; A reflux component (12) is arranged on the inner wall of the mixing drum (1) and is used for mixing raw materials at different positions; A cooling assembly (36) is arranged on the outer wall of the stirring shaft (9) and is used to reduce the heat generated by the spiral stirring paddle (11) during the stirring process.

2. The ion exchange extraction device for purifying lysozyme from egg white according to claim 1, characterized in that: The reflux assembly (12) comprises a plurality of reflux pipes (121) arranged on the inner wall of the mixing drum (1), the reflux pipe (121) being an annular structure, the inner wall of the reflux pipe (121) being fixedly connected to a plurality of reflux heads (122), the right side wall of the mixing drum (1) being fixedly connected to a mounting frame (13), the inner wall of the mounting frame (13) being fixedly connected to a first pump (14), the feed end of the first pump (14) being fixedly connected to a suction pipe (15), the pipe wall of the suction pipe (15) being fixedly connected to a plurality of transverse pipes (16), the end of the transverse pipe (16) away from the suction pipe (15) passing through the mixing drum (1) and being connected to the reflux pipe (121), the transverse pipe (16) being provided with a first regulating valve (17), the discharge end of the first pump (14) being fixedly connected to a discharge pipe (18), and the discharge end of the first pump (14) being fixedly connected to a discharge pipe (18). ), the inner wall of the mixing drum (1) is connected to a liquid discharge pipe (19), the liquid discharge pipe (19) is an annular structure, the inner wall of the liquid discharge pipe (19) is fixedly connected to a plurality of liquid discharge heads (20), the upper end of the discharge pipe (18) passes through the mixing drum (1) and is connected to the liquid discharge pipe (19), the upper side wall of the mixing drum (1) is fixedly connected to a second pump (21) via a bracket, the mixing shaft (9) is a hollow structure, the rod wall of the mixing shaft (9) is connected to a plurality of liquid inlet heads (22), the liquid inlet pipe (23) of the second pump (21) is rotatably connected to the inner wall of the mixing shaft (9) via a sealing bearing, the discharge end of the second pump (21) is connected to the liquid discharge pipe (19), and the liquid inlet pipe (23) of the second pump (21) and the pipe wall of the suction pipe (15) are both connected to a detection component (24).

3. The ion exchange extraction device for purifying lysozyme from egg white according to claim 2, characterized in that: The detection assembly (24) comprises a mesh plate (241) arranged on the inner walls of the suction pipe (15) and the liquid inlet pipe (23); the inner walls of the suction pipe (15) and the liquid inlet pipe (23) are connected to the mesh plate (241) via an elastic rod; the outer walls of the suction pipe (15) and the liquid inlet pipe (23) are connected to a detection box (242) via a bracket; the inner walls of the two detection boxes (242) are connected to a pressure sensor (25); the detection end of the pressure sensor (25) is connected to a piston plate (26); The upper side walls of the two detection boxes (242) are fixedly connected to detection tubes (27); the ends of the two detection tubes (27) away from the detection boxes (242) pass through the suction tube (15) and the liquid inlet tube (23) respectively, and are connected to a compression tube (28); the ends of the compression tubes (28) away from the detection tubes (27) are connected to the mesh plate (241); the pressure sensor (25) is electrically connected to the PLC controller (2); and the upper side wall of the mixing drum (1) is fixedly connected to an analysis component (29).

4. The ion exchange extraction device for purifying lysozyme from egg white according to claim 3, characterized in that: The analysis component (29) comprises two analysis boxes (291) arranged above the mixing drum (1); the two analysis boxes (291) are connected via a bracket; the right inner walls of the two analysis boxes (291) are connected to a moving plate (292) via a spring; the right inner walls of the two analysis boxes (291) are fixedly connected to an electromagnetic block (30); the right wall of the moving plate (292) is fixedly connected to a permanent magnet block (31); the side walls on the opposite side of the two moving plates (292) are fixedly connected to an analysis rod (32); and the two The opposite ends of the analysis rod (32) pass through the analysis box (291); a sliding opening matching the analysis rod (32) is provided on the side wall of the analysis box (291); a conductive seat (33) is fixedly connected to the upper end of the analysis rod (32) located at the lower side; the conductive seat (33) is electrically connected to an external power supply; a connecting frame (34) is fixedly connected to the lower end of the analysis rod (32); two conductive plates (35) are fixedly connected to the inner wall of the connecting frame (34); and both conductive plates (35) are electrically connected to the PLC controller (2).

5. The ion exchange extraction device for purifying lysozyme from egg white according to claim 1, characterized in that: The cooling assembly (36) comprises an annular water tank (361) fixedly sleeved on the outer wall of the stirring shaft (9); a water pump (362) is fixedly connected to the upper side wall of the annular water tank (361); a liquid inlet end of the water pump (362) is located in the annular water tank (361); a liquid outlet end of the water pump (362) is fixedly connected to a water supply pipe (37); two connecting pipes (38) are fixedly connected between the stirring shaft (9) and the spiral stirring paddle (11); the spiral stirring paddle (11) is of a hollow structure; the lower end of the water supply pipe (37) is connected to the upper water supply pipe (37); one end of the connecting pipe (38) located at the lower end and close to the stirring shaft (9) is connected to a return pipe (39); the upper end of the return pipe (39) is connected to the annular water tank (361).

6. The ion exchange extraction device for purifying lysozyme from egg white according to claim 2, characterized in that: A protective cover (40) is fixedly connected to the upper side wall of the mixing drum (1), and the second pump (21) and the mixing motor (10) are both located inside the protective cover (40).

7. The ion exchange extraction device for purifying lysozyme from egg white according to claim 5, characterized in that: A plurality of semiconductor refrigeration plates (41) are plugged into the upper side wall of the annular water tank (361), and the heat release ends of the plurality of semiconductor refrigeration plates (41) all extend out of the annular water tank (361).

8. The ion exchange extraction device for purifying lysozyme from egg white according to claim 1, characterized in that: A temperature sensor (42) is provided on the surface of the spiral stirring blade (11), and the temperature sensor (42) is electrically connected to the PLC controller (2).

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