Experimental culture device for tumor cells

By designing a tumor cell experimental culture device that includes regulation, circulation and cooling components, the shortcomings of traditional devices in the control of environmental parameters and humidification process are solved, and a more efficient and safer tumor cell culture experiment is achieved.

CN119875829AInactive Publication Date: 2025-04-25THE SECOND HOSPITAL OF NANJING
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Patent Information

Application Number
CN202411850187.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-16
Publication Date
2025-04-25
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

The existing tumor cell experimental culture devices have shortcomings in the control of environmental parameters such as temperature, humidity, and gas concentration, and cannot simulate the complex microenvironment of tumor cells in the body. The single use of clean water during humidification increases the cumbersomeness of experimental operations and the risk of operation errors.

Method used

An experimental culture device for tumor cells including support blocks, incubators, regulation components, circulation components and cooling components was designed. Through the cooperation of the first and second adjustment components, flexible adjustment of the space is achieved; the circulation component realizes the recycling of clean water; and the cooling component realizes humidification effect through water vapor cooling.

Benefits of technology

The device can control the culture environment more accurately, improve the reliability and repeatability of experimental results, reduce the possibility of operational errors, and reduce the cultivation cost.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention discloses an experimental culture device for tumor cells, and belongs to the technical field of cell culture.The experimental culture device for the tumor cells comprises supporting blocks, an incubator is fixedly connected between the tops of the multiple supporting blocks, and a first adjusting assembly and a second adjusting assembly are installed in the incubator; a humidity sensor is installed on the top of the inner wall of the incubator, two hinges are fixedly connected to one side of the outer wall of the incubator, a switch door is rotationally connected between the two hinges, a switch button, an adjusting button and a display screen are installed on the top of the front end of the incubator, and a circulating assembly and a cooling assembly are installed on the outer wall of the incubator. The circulating assembly is arranged; the continuous circulation can buffer the influence of external factors on the humidity, the humidity consistency can be better maintained, the adverse influence of humidity fluctuation on the growth of tumor cells is avoided, the reliability and repeatability of experimental results are improved, and meanwhile the culture cost is reduced.
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Description

Technical Field

[0001] The invention belongs to the technical field of cell culture, and in particular relates to a device for culturing tumor cells in experiments. Background Art

[0002] In modern medical and biological research, the study of tumor cells is of vital importance. Understanding the growth, differentiation, invasion and metastasis mechanisms of tumor cells is of key significance for the development of effective tumor treatment methods. In the past, the technology of experimental culture of tumor cells was relatively simple and rough, and the culture conditions were difficult to accurately control, resulting in the accuracy and repeatability of experimental results being limited. With the continuous advancement of science and technology, the requirements for tumor cell culture devices are also increasing. Traditional culture equipment has deficiencies in the control of environmental parameters such as temperature, humidity, and gas concentration, and cannot simulate the complex microenvironment of tumor cells in the body.

[0003] Most of the existing devices use a single-use method for clean water during the humidification process, so that the clean water needs to be replaced every time an experiment is performed, which increases the complexity of the experimental operation. The operator needs to spend time and energy to prepare new clean water, and needs to be careful during the replacement process to avoid contaminating the experimental culture device or affecting the progress of the experiment. This not only reduces work efficiency, but also increases the risk of operational errors. Summary of the invention

[0004] The technical problem to be solved by the present invention is to overcome the disadvantages of the above-mentioned prior art and provide a device for culturing tumor cells in experiments.

[0005] The technical solution adopted to solve the above technical problems is: a device for tumor cell experimental culture, including support blocks, a culture box fixedly connected between the tops of multiple support blocks, a first adjustment component and a second adjustment component installed inside the culture box, a humidity sensor installed on the top of the inner wall of the culture box, two hinges fixedly connected to one side of the outer wall of the culture box, a switch door rotatably connected between the two hinges, tempered glass installed in the center of the switch door, a switch button, an adjustment button and a display screen installed on the top of the front end of the culture box, and a circulation component and a cooling component installed on the outer wall of the culture box.

[0006] Furthermore, the first adjustment component includes a plurality of first mounting grooves opened on the inner wall of the incubator, a first guide rod is fixedly connected inside each of the plurality of first mounting grooves, a first threaded rod is rotatably connected inside one of the first mounting grooves, a first motor is installed on the top of the first threaded rod, a first support plate is slidably connected between the plurality of first guide rods and the first threaded rods, two first reinforcing ribs are fixedly connected to the inner wall of the first support plate, two first slide grooves are opened on the top of the first support plate, and a first partition plate is slidably connected between the two first slide grooves.

[0007] Through the above technical solution, the first motor is started first, so that the first motor drives the first threaded rod to rotate, and then the first threaded rod drives the first support plate to move up and down along the outer walls of multiple first guide rods. At the same time, the first support plate drives the first partition to move synchronously until it reaches the specified position, thereby achieving the effect of adjusting the size of the space.

[0008] Furthermore, the second adjustment component includes a plurality of second mounting grooves opened on the inner wall of the incubator, a second guide rod is fixedly connected inside each of the plurality of second mounting grooves, a second threaded rod is rotatably connected inside one of the second mounting grooves, a second motor is installed on the top of the second threaded rod, a second support plate is slidably connected between the plurality of second guide rods and the second threaded rod, two second reinforcing ribs are fixedly connected between the inner walls of the second support plate, two second slide grooves are opened on the top of the second support plate, and a second partition is slidably connected between the inner walls of the two second slide grooves.

[0009] Through the above technical solution, the second motor is first started so that the second motor drives the second threaded rod to rotate, and then the second threaded rod drives the second support plate to move up and down along the outer walls of multiple second guide rods. At the same time, the second support plate drives the second partition to move synchronously until it moves to the specified position, and then cooperates with the first adjustment component to achieve the effect of maximizing space utilization.

[0010] Furthermore, a sealing groove is provided at the rear end of the switch door, a sealing strip is fixedly connected to the front end of the incubator, and the inner wall of the sealing groove fits with the outer wall of the sealing strip.

[0011] Through the above technical solution, the outside air is effectively prevented from entering the incubator, the invasion of external impurities, microorganisms, etc. is reduced, a pure and stable culture environment is provided for tumor cells, and gas leakage in the incubator is prevented, ensuring that the concentration of oxygen, carbon dioxide and other gases in the incubator is maintained at the set level, which is conducive to maintaining the normal metabolism and growth of cells.

[0012] Furthermore, the circulation component includes a first circulation pipe and a first storage box fixedly connected to one side of the incubator, a second circulation pipe is fixedly connected to the rear end of the outer wall of the first storage box, a centrifugal pump is installed on the second circulation pipe, a third circulation pipe is installed at the rear end of the centrifugal pump, a connecting block is fixedly connected to the rear end of the incubator, a stirring box is fixedly connected to the rear end of the connecting block, a third motor is installed on the hinge side of the outer wall of the stirring box, a stirring shaft is fixedly connected to the output shaft of the third motor, a plurality of high-temperature stirring rods are fixedly connected to the outer wall of the stirring shaft, and a first water inlet is opened at the top of the outer wall of the stirring box.

[0013] Through the above technical solution, clean water is added to the mixing box through the first water inlet, and then the water at the bottom of the incubator is transported to the first storage box through the first circulation pipe, and then the water in the first storage tank is transported to the third circulation pipe through a centrifugal pump, and then the clean water is transported to the mixing box through the third circulation pipe for stirring, thereby achieving a circulation effect. At the same time, during the stirring process, the high-temperature stirring rod will evaporate part of the water, and then form water vapor to be transported to the cooling component.

[0014] Furthermore, the plurality of high-temperature stirring rods are arranged in an S shape, and one side of the third circulation pipe is fixedly connected to a corresponding surface of the stirring box.

[0015] Through the above technical solution, the S-shaped arrangement can make the high-temperature stirring rod have a wider action area in the mixing box, reduce the stirring dead corners, and ensure that the materials are more fully mixed. The fixed connection ensures the stability of the third circulation pipe during operation, reduces pipeline vibration and displacement, and reduces the risk of leakage caused by loose connections.

[0016] Furthermore, the cooling component includes a first conveying pipe fixedly connected to the mixing box, a fan is installed on the outer wall of the first conveying pipe, a cooling pipe is fixedly connected to the front end of the outer wall of the first conveying pipe, a cooling box is fixedly connected to the rear end of the mixing box, and a second water inlet is arranged on the top of the cooling box.

[0017] Through the above technical solution, the fan is first started to transport the water vapor generated in the mixing box to the first delivery pipe, and then clean water is added to the cooling box through the second water inlet. Then the water vapor will be transported to the cooling box along the first delivery pipe. Since the cooling box is immersed in clean water, the cooling effect is achieved. Finally, the cooled water vapor will be transported to the incubator to achieve the humidification effect.

[0018] Furthermore, a limiting groove is provided at the center of the top of the connecting block, and the cooling pipe passes through the limiting groove.

[0019] Through the above technical solution, the limit groove can play a certain positioning role for the cooling pipe, so that it can remain stable in a specific position and prevent the cooling pipe from unnecessary movement or shaking during operation. This helps to ensure the accuracy of the relative position of the cooling pipe and other components and ensure the normal operation of the cooling system.

[0020] Furthermore, a reinforcement component is installed at the rear end of the inner wall of the incubator, and the reinforcement component includes two third slide grooves opened at the rear end of the inner wall of the incubator, and the inner walls of the two third slide grooves are slidably connected with a plurality of sliders, and a connecting plate is fixedly connected between the front ends of every two of the sliders, and the front ends of the plurality of connecting plates are fixedly connected with reinforcement plates, and the reinforcement plates are provided with a plurality of reinforcement holes.

[0021] Through the above technical solution, the second adjustment component is first moved to the specified position. At the same time, multiple connecting plates will move synchronously with the corresponding second adjustment components, and then the culture tank is placed into the corresponding reinforcement hole to achieve the effect of reinforcing the culture tank.

[0022] The beneficial effects of the present invention are as follows: (1) The present invention sets a circulation component; continuous circulation can buffer the impact of external factors on humidity, can better maintain the consistency of humidity, and avoid the adverse effects of humidity fluctuations on tumor cell growth, which not only improves the reliability and repeatability of experimental results, but also reduces the culture cost; (2) The present invention sets a first adjustment component and a second adjustment component; it can flexibly adjust the partition spacing according to culture vessels of different sizes and experimental requirements, fully utilize the space inside the culture device, and also facilitate a variety of different types of tumor cell culture experiments in the same device, thereby increasing the scope of use of the equipment; (3) The present invention sets a reinforcement component to make the culture tank more stable during operation, facilitate the experimenter to add culture medium, take samples, etc., and reduce the possibility of operational errors. At the same time, it effectively prevents the culture tank from being displaced or tipped over due to slight collisions or vibrations during the experiment, thereby ensuring the stability of the culture environment and reducing interference with cell culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 is an appearance diagram of the present invention;

[0024] Figure 2 It is a front view of the present invention;

[0025] Figure 3 yes Figure 2 Middle AA section view;

[0026] Figure 4 is a schematic diagram of the structure of the first adjustment component and the second adjustment component of the present invention;

[0027] Figure 5 It is a schematic diagram of the structure of the circulation component and the cooling component of the present invention;

[0028] Figure 6 is a bottom view of the circulation component and the cooling component of the present invention;

[0029] Figure 7 yes Figure 6 Middle BB section view;

[0030] Figure 8 It is a schematic diagram of the structure of the reinforcement component of the present invention.

[0031] Figure numerals: 1, support block; 2, incubator; 3, first adjustment component; 301, first mounting groove; 302, first guide rod; 303, first threaded rod; 304, first motor; 305, first support plate; 306, first reinforcing rib; 307, first slide groove; 308, first partition; 4, second adjustment component; 401, second mounting groove; 402, second guide rod; 403, second threaded rod; 404, second motor; 405, second support plate; 406, second reinforcing rib; 407, second slide groove; 408, second partition; 5, humidity sensor; 6, hinge; 7, switch door; 701, sealing groove; 702, sealing strip; 8, tempered glass; 9, switch button; 10, adjustment 1. button; 11. display screen; 12. circulation component; 1201. first circulation pipe; 1202. first storage box; 1203. second circulation pipe; 1204. centrifugal pump; 1205. third circulation pipe; 1206. connecting block; 1207. stirring box; 1208. third motor; 1209. stirring shaft; 1210. high temperature stirring rod; 1211. first water inlet; 13. cooling component; 1301. first conveying pipe; 1302. fan; 1303. cooling pipe; 1304. cooling box; 1305. second water inlet; 14. reinforcement component; 1401. third slide; 1402. slider; 1403. connecting plate; 1404. reinforcement plate; 1405. reinforcement hole. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0033] like Figure 1 - Figure 8As shown, a tumor cell experimental culture device of this embodiment includes a support block 1, a culture box 2 is fixedly connected between the tops of multiple support blocks 1, a first adjustment component 3 and a second adjustment component 4 are installed inside the culture box 2, the first adjustment component 3 includes a plurality of first installation grooves 301 opened on the inner wall of the culture box 2, and a first guide rod 302 is fixedly connected inside the plurality of first installation grooves 301, a first threaded rod 303 is rotatably connected inside one of the first installation grooves 301, a first motor 304 is installed on the top of the first threaded rod 303, and a plurality of first guide rods 302 slide between the first threaded rod 303 and the first threaded rod 303. A first support plate 305 is connected, two first reinforcing ribs 306 are fixedly connected to the inner wall of the first support plate 305, two first slide grooves 307 are arranged on the top of the first support plate 305, and a first partition plate 308 is slidably connected between the two first slide grooves 307; firstly, the first motor 304 is started, so that the first motor 304 drives the first threaded rod 303 to rotate, and then the first threaded rod 303 drives the first support plate 305 to move up and down along the outer walls of the plurality of first guide rods 302, and at the same time, the first support plate 305 drives the first partition plate 308 to move synchronously until it reaches the specified position, thereby completing the effect of adjusting the size of the space;

[0034] like Figure 3 - Figure 4 As shown, the second adjustment component 4 includes a plurality of second mounting grooves 401 opened on the inner wall of the incubator 2, and a second guide rod 402 is fixedly connected inside the plurality of second mounting grooves 401, a second threaded rod 403 is rotatably connected inside one of the second mounting grooves 401, a second motor 404 is installed on the top of the second threaded rod 403, a second support plate 405 is slidably connected between the plurality of second guide rods 402 and the second threaded rod 403, two second reinforcing ribs 406 are fixedly connected between the inner walls of the second support plate 405, and the top of the second support plate 405 is opened. Two second slide grooves 407 are provided, and a second partition plate 408 is slidably connected between the inner walls of the two second slide grooves 407; firstly, the second motor 404 is started, so that the second motor 404 drives the second threaded rod 403 to rotate, and then the second threaded rod 403 drives the second support plate 405 to move up and down along the outer walls of the plurality of second guide rods 402, and at the same time, the second support plate 405 drives the second partition plate 408 to move synchronously until it moves to the specified position, and then through the mutual cooperation with the first adjustment component 3, the effect of maximizing the utilization of space is achieved;

[0035] like Figure 3 , Figure 5 , Figure 6 and Figure 7As shown, a humidity sensor 5 is installed on the top of the inner wall of the incubator 2, two hinges 6 are fixedly connected to one side of the outer wall of the incubator 2, a switch door 7 is rotatably connected between the two hinges 6, a sealing groove 701 is provided at the rear end of the switch door 7, a sealing strip 702 is fixedly connected to the front end of the incubator 2, and the inner wall of the sealing groove 701 fits with the outer wall of the sealing strip 702; the outside air is effectively prevented from entering the inside of the incubator 2, the invasion of external impurities, microorganisms, etc., a pure and stable culture environment is provided for tumor cells, and the gas leakage in the incubator 2 is prevented, so as to ensure that the concentration of oxygen, carbon dioxide and other gases in the incubator 2 is maintained at a set level, which is conducive to maintaining the normal cell generation Xie and growth, a tempered glass 8 is installed in the center of the switch door 7, a switch button 9, an adjustment button 10 and a display screen 11 are installed on the top of the front end of the incubator 2, a circulation component 12 and a cooling component 13 are installed on the outer wall of the incubator 2, the circulation component 12 includes a first circulation pipe 1201 and a first storage box 1202 fixedly connected to one side of the incubator 2, a second circulation pipe 1203 is fixedly connected to the rear end of the outer wall of the first storage box 1202, a centrifugal pump 1204 is installed on the second circulation pipe 1203, a third circulation pipe 1205 is installed at the rear end of the centrifugal pump 1204, a connection block 1206 is fixedly connected to the rear end of the incubator 2, and a stirring box 1 is fixedly connected to the rear end of the connection block 1206. 207, a third motor 1208 is installed on the side of the outer wall of the stirring box 1207 close to the hinge 6, the output shaft of the third motor 1208 is fixedly connected to the stirring shaft 1209, the outer wall of the stirring shaft 1209 is fixedly connected to a plurality of high-temperature stirring rods 1210, and a first water inlet 1211 is opened on the top of the outer wall of the stirring box 1207; clean water is added to the stirring box 1207 through the first water inlet 1211, and then the water at the bottom of the incubator 2 is transported to the first storage box 1202 through the first circulation pipe 1201, and then the water in the first storage box 1202 is transported to the third circulation pipe 1205 through the centrifugal pump 1204, and then the clean water is transported to The stirring is performed in the stirring box 1207 to achieve a circulation effect. At the same time, during the stirring process, the high-temperature stirring rod 1210 will evaporate a portion of the water, thereby forming water vapor and transporting it to the cooling component 13. The multiple high-temperature stirring rods 1210 are arranged in an S-shaped arrangement, and one side of the third circulation pipe 1205 is fixedly connected to the corresponding surface of the stirring box 1207; the S-shaped arrangement can make the high-temperature stirring rod 1210 have a wider action area in the stirring box 1207, reduce the stirring dead angle, and ensure that the materials are more fully mixed. The fixed connection ensures the stability of the third circulation pipe 1205 during operation, reduces pipeline vibration and displacement, and reduces the risk of leakage caused by loose connections;

[0036] like Figure 5 , Figure 6 and Figure 7As shown, the cooling component 13 includes a first conveying pipe 1301 fixedly connected to the mixing box 1207, a fan 1302 is installed on the outer wall of the first conveying pipe 1301, a cooling pipe 1303 is fixedly connected to the front end of the outer wall of the first conveying pipe 1301, and a cooling box 1304 is fixedly connected to the rear end of the mixing box 1207. A second water inlet 1305 is provided on the top of the cooling box 1304; first, the fan 1302 is started to convey the water vapor generated in the mixing box 1207 to the first conveying pipe 1301, and then clean water is added to the cooling box 1304 through the second water inlet 1305, and then the water vapor will flow along the first conveying pipe 1301. The tube 1301 is transported to the cooling box 1304. Since the cooling box 1304 is immersed in clean water, the cooling effect is achieved. Finally, the water vapor after cooling will be transported to the incubator 2 to achieve the humidification effect. A limiting groove is provided at the top center of the connecting block 1206, and the cooling tube 1303 passes through the limiting groove; the limiting groove can play a certain positioning role for the cooling tube 1303, so that it remains stable at a specific position, and prevents the cooling tube 1303 from unnecessary movement or shaking during operation, which helps to ensure the relative position of the cooling tube 1303 and other components is accurate, and ensure the normal operation of the cooling system;

[0037] like Figure 8 As shown, a reinforcement component 14 is installed at the rear end of the inner wall of the incubator 2, and the reinforcement component 14 includes two third slide grooves 1401 opened at the rear end of the inner wall of the incubator 2, and the inner walls of the two third slide grooves 1401 are slidably connected with multiple sliders 1402, and a connecting plate 1403 is fixedly connected between the front ends of each two sliders 1402, and the front ends of the multiple connecting plates 1403 are fixedly connected with reinforcement plates 1404, and the reinforcement plates 1404 are each provided with multiple reinforcement holes 1405; first, the second adjustment component 4 is moved to the specified position, and at the same time, the multiple connecting plates 1403 will move synchronously with the corresponding second adjustment component 4, and then the culture tank is placed in the corresponding reinforcement hole 1405, so that the effect of reinforcing the culture tank can be achieved.

[0038] The working principle of this embodiment is as follows. First, the first motor 304 is started, so that the first motor 304 drives the first threaded rod 303 to rotate, and then the first threaded rod 303 drives the first support plate 305 to move up and down along the outer walls of the plurality of first guide rods 302. At the same time, the first support plate 305 drives the first baffle 308 to move synchronously until it reaches the specified position, thereby completing the effect of adjusting the size of the space. Then, the second motor 404 is started, so that the second motor 404 drives the second threaded rod 403 to rotate, and then the second threaded rod 403 drives the second support plate 405 to move up and down along the outer walls of the plurality of second guide rods 402. At the same time, the second support plate 405 drives the second baffle 408 to move synchronously until it moves to the specified position. Then, through the mutual cooperation with the first adjusting component 3, the effect of maximizing the utilization of the space is achieved. The plurality of connecting plates 1403 will move synchronously with the corresponding second adjusting component 4, and then the culture tank is placed in the corresponding reinforcement hole 1405, so as to achieve the effect of reinforcing the culture tank. Then, through the first water adding The clean water is added into the stirring box 1207 through the inlet 1211, and then the water at the bottom of the incubator 2 is transported to the first storage box 1202 through the first circulation pipe 1201, and then the water in the first storage box 1202 is transported to the third circulation pipe 1205 through the centrifugal pump 1204, and then the clean water is transported to the stirring box 1207 through the third circulation pipe 1205 for stirring, thereby achieving a circulation effect. At the same time, during the stirring process, the high-temperature stirring rod 1210 will evaporate part of the water, thereby forming water vapor and transporting it to the cooling tank 1207. The temperature component 13 is then controlled in real time by the humidity sensor 5, and the fan 1302 is started to transport the water vapor generated in the stirring box 1207 to the first delivery pipe 1301. Clean water is then added to the cooling box 1304 through the second water inlet 1305. The water vapor is then transported along the first delivery pipe 1301 to the cooling box 1304. Since the cooling box 1304 is immersed in clean water, a cooling effect is achieved. Finally, the cooled water vapor is transported to the incubator 2 to achieve a humidification effect.

[0039] The above description is only a preferred embodiment of the present invention and is not intended to limit the protection scope of the present invention.

Claims

1. A device for culturing tumor cells in an experiment, comprising a support block (1), characterized in that: An incubator (2) is fixedly connected between the tops of the plurality of support blocks (1), a first adjustment component (3) and a second adjustment component (4) are installed inside the incubator (2), a humidity sensor (5) is installed on the top of the inner wall of the incubator (2), two hinges (6) are fixedly connected to one side of the outer wall of the incubator (2), a switch door (7) is rotatably connected between the two hinges (6), a tempered glass (8) is installed at the center of the switch door (7), a switch button (9), an adjustment button (10) and a display screen (11) are installed on the top of the front end of the incubator (2), a circulation component (12) and a cooling component (13) are installed on the outer wall of the incubator (2), and a reinforcement component (14) is installed on the rear end of the inner wall of the incubator (2).

2. The device for culturing tumor cells according to claim 1, characterized in that: The first adjustment component (3) comprises a plurality of first mounting grooves (301) provided on the inner wall of the incubator (2), each of the plurality of first mounting grooves (301) being fixedly connected with a first guide rod (302), a first threaded rod (303) being rotatably connected to the interior of one of the first mounting grooves (301), a first motor (304) being installed on the top of the first threaded rod (303), a first support plate (305) being slidably connected between the plurality of first guide rods (302) and the first threaded rod (303), two first reinforcing ribs (306) being fixedly connected to the inner wall of the first support plate (305), two first slide grooves (307) being provided on the top of the first support plate (305), a first partition plate (308) being slidably connected between the two first slide grooves (307).

3. The device for culturing tumor cells according to claim 1, characterized in that: The second adjustment component (4) comprises a plurality of second mounting grooves (401) provided on the inner wall of the incubator (2), the plurality of second mounting grooves (401) being fixedly connected with a second guide rod (402) inside, a second threaded rod (403) being rotatably connected inside one of the second mounting grooves (401), a second motor (404) being installed on the top of the second threaded rod (403), a second support plate (405) being slidably connected between the plurality of second guide rods (402) and the second threaded rod (403), two second reinforcing ribs (406) being fixedly connected between the inner walls of the second support plate (405), two second slide grooves (407) being provided on the top of the second support plate (405), and a second partition plate (408) being slidably connected between the inner walls of the two second slide grooves (407).

4. The device for culturing tumor cells according to claim 1, characterized in that: A sealing groove (701) is provided at the rear end of the switch door (7), a sealing strip (702) is fixedly connected to the front end of the incubator (2), and the inner wall of the sealing groove (701) fits with the outer wall of the sealing strip (702).

5. The device for culturing tumor cells according to claim 1, characterized in that: The circulation assembly (12) comprises a first circulation pipe (1201) fixedly connected to one side of the incubator (2) and a first storage box (1202); a second circulation pipe (1203) is fixedly connected to the rear end of the outer wall of the first storage box (1202); a centrifugal pump (1204) is installed on the second circulation pipe (1203); a third circulation pipe (1205) is installed at the rear end of the centrifugal pump (1204); a connection block (1206) is fixedly connected to the rear end of the incubator (2); 206), the rear end of the connecting block (1206) is fixedly connected to a stirring box (1207), a third motor (1208) is installed on the outer wall of the stirring box (1207) close to the hinge (6), the output shaft of the third motor (1208) is fixedly connected to a stirring shaft (1209), the outer wall of the stirring shaft (1209) is fixedly connected to a plurality of high-temperature stirring rods (1210), and a first water inlet (1211) is provided on the top of the outer wall of the stirring box (1207).

6. The device for culturing tumor cells according to claim 5, characterized in that: The plurality of high-temperature stirring rods (1210) are arranged in an S shape, and one side of the third circulation pipe (1205) is fixedly connected to a corresponding surface of the stirring box (1207).

7. The device for culturing tumor cells according to claim 5, characterized in that: The cooling component (13) comprises a first conveying pipe (1301) fixedly connected to a mixing box (1207); a fan (1302) is installed on the outer wall of the first conveying pipe (1301); a cooling pipe (1303) is fixedly connected to the front end of the outer wall of the first conveying pipe (1301); a cooling box (1304) is fixedly connected to the rear end of the mixing box (1207); and a second water inlet (1305) is arranged on the top of the cooling box (1304).

8. The device for culturing tumor cells according to claim 7, characterized in that: A limiting groove is provided at the top center of the connection block (1206), and the cooling pipe (1303) passes through the limiting groove.

9. The device for culturing tumor cells according to claim 1, characterized in that: The reinforcement component (14) comprises two third slide grooves (1401) provided at the rear end of the inner wall of the incubator (2); the inner walls of the two third slide grooves (1401) are slidably connected with a plurality of sliders (1402); a connecting plate (1403) is fixedly connected between the front ends of each two sliders (1402); the front ends of the plurality of connecting plates (1403) are fixedly connected with a reinforcement plate (1404); and the reinforcement plate (1404) is provided with a plurality of reinforcement holes (1405).