Single cell cloning cultivation device

Abstract

The invention relates to the technical field of single cell clone cultivation, in particular to a single cell clone cultivation device which comprises a base, a U-shaped table is fixedly connected to the upper side of the base, a cultivation device body is fixedly arranged on the upper side of the U-shaped table, a driven rotating shaft is rotationally installed in the cultivation device body, and discs are connected to the outer surface of the driven rotating shaft in an array mode. The placement plates can be limited or released through movement adjustment of the convex plates, the placement plates can be jacked up in cooperation with upward movement of the movable top plate, limitation on all the placement plates can be released at the same time, the placement plates can be directly taken or fixed in the follow-up process, operation is easy and convenient, and practicability is high. The flexibility and convenience of the single-cell clone cultivation device during use are improved, a pinion is adopted to drive a large gear to rotate, so that a driven rotating shaft rotates gently, the situation that single-cell clone cultivation is affected due to the fact that the rotating speed is too high is avoided, and the positions of the culture dishes are adjusted so that all the culture dishes can be observed.

Description

Technical Field

[0001] The invention relates to the technical field of single cell cloning and cultivation, in particular to a single cell cloning and cultivation device. Background Art

[0002] Cell culture is also called cell cloning technology, in which the cloning cell culture method is a technology used to extract and amplify single immune cell clones. This method can separate a specific immune cell (such as lymphocytes, recombinant antibodies, etc.) in the form of a single cell, and then hybridize and differentiate in the culture medium. In order to cultivate single cells, a single cell cloning culture device is required.

[0003] A Chinese patent (application number CN202121221871.8) discloses a stem cell culture device, including a box body, bases are respectively provided on the walls around the bottom of the box body, a sealed door is provided on the wall on one side of the front end of the box body, an outer door is also provided on the wall on the side close to the sealed door at the front end of the box body, a drainage hole is also opened in the wall on one side below the front end of the box body, fixed frames are respectively provided on the front and rear end walls on both sides of the box body, a shelf is provided between the two fixed frames, and a clamping mechanism is provided on the rear end wall on the top of the shelf, and the clamping mechanism includes a fixing rod, a connecting rod and a pressure plate.

[0004] Although the above-mentioned stem cell culture device can clamp and fix culture dishes or culture bottles of different heights, the existing culture device usually fixes multiple single-cell cloning culture dishes. If the fixing method is cumbersome, it is necessary to limit or release the limit one by one, which is time-consuming and makes it impossible to conveniently take or fix the single-cell cloning culture dish, thereby affecting the flexibility and convenience of the single-cell cloning culture device when using it, and it is not convenient to use. Summary of the invention

[0005] The object of the present invention is to provide a single cell cloning culture device to solve the problems raised in the above background technology.

[0006] The purpose of the present invention can be achieved through the following technical solutions:

[0007] A single cell cloning cultivation device comprises a base, a U-shaped platform is fixedly connected to the upper side of the base, a cultivation device body is fixedly arranged on the upper side of the U-shaped platform, a driven shaft is rotatably installed inside the cultivation device body, a disk is connected to the outer surface of the driven shaft in an array, and an adapter slot is opened on the upper side of the disk;

[0008] A placement plate is clamped inside the adapter slot, a culture dish is fixed inside the placement plate, an array-distributed U-shaped plate is fixedly connected to one side of the interior of the cultivation device body, two symmetrically arranged movable top plates are slidably connected to the upper side of the U-shaped plate, a spring 1 is fixedly installed between the two movable top plates and the U-shaped plate, an array-distributed cam rotating rod is rotatably connected to one side of the interior of the cultivation device body, a first gear is rotatably connected to the rear side of the cam rotating rod, and a gear rotating rod is rotatably connected to the rear side of the cultivation device body.

[0009] Preferably, a chain is sleeved between the first gear and the gear rotating rod, the rear side of the gear rotating rod is fixedly connected to the first servo motor through a motor mounting plate, two symmetrically arranged hollow circular grooves are opened inside the disc, four self-locking universal wheels are fixedly connected to the lower side of the base, and two symmetrically arranged sterilization lamps are fixedly provided inside the main body of the cultivation device.

[0010] Preferably, a first fixing plate is fixedly installed on the upper side of the placing plate, handles are fixedly provided on the upper sides of the two first fixing plates, two symmetrically arranged second fixing plates are fixedly connected to the upper side of the disc, a limiting slide bar for fixing the first fixing plate is slidably installed on one side of the two second fixing plates, a spring 2 is fixedly connected between the two limiting slide bars and the second fixing plate, one side of the limiting slide bar passes through the second fixing plate and extends to the other side of the second fixing plate, and a U-shaped seat is fixedly provided on one side of the two limiting slide bars.

[0011] Preferably, a mounting seat is fixedly installed at the bottom end of the main body of the cultivation device, a bidirectional threaded rotating rod is rotatably installed on the inner side of the mounting seat, the outer surface of the bidirectional threaded rotating rod is threadedly connected with two symmetrically arranged vertical plates, one side of the two vertical plates is fixedly installed with an electric push rod, one side of the two electric push rods is fixedly provided with an extension plate, and one side of the two extension plates is connected with a convex plate in an array.

[0012] Preferably, a bevel gear shaft is rotatably connected to one side inside the cultivation device body, the right side of the bidirectional threaded rotating rod penetrates the mounting seat and extends to the right side of the mounting seat, and a driven bevel gear is fixedly connected to the right side of the bidirectional threaded rotating rod, the bevel gear shaft and the driven bevel gear mesh with each other, and the meshing method is bevel gear meshing, and two limiting round rods are fixedly connected to the inner side of the mounting seat, and the outer surfaces of the two limiting round rods are slidably connected to the inside of the vertical plate, and the rear side of the bevel gear shaft penetrates the cultivation device body and extends to the rear side of the cultivation device body, and the rear side of the bevel gear shaft is fixedly connected to the third servo motor through the motor mounting plate.

[0013] Preferably, the placement plate is internally slidably connected to an array-distributed telescopic arc plate, a spring three is fixedly connected between the telescopic arc plate and the placement plate, the telescopic arc plate is used to clamp the culture dish, and two U-shaped handles are fixedly installed on the upper side of the placement plate.

[0014] Preferably, a driven gear is fixedly connected to the outer surface of the driven rotating shaft, a driving gear rod is rotatably installed at the bottom end of the inner body of the cultivation device, the driving gear rod and the driven gear are meshed with each other, the lower side of the driving gear rod penetrates the cultivation device body and extends to the lower side of the U-shaped platform, and the lower side of the driving gear rod is fixedly connected to a second servo motor through a motor mounting plate.

[0015] Preferably, two support plates are fixedly connected to the front side of the cultivation device body, a sealed door is rotatably connected between the two support plates, a transparent glass window is fixedly provided inside the sealed door, and two brackets are fixedly connected to the rear side of the U-shaped platform, and a rubber rod is fixedly installed between the two brackets.

[0016] Preferably, the lower side of the movable top plate passes through the U-shaped plate and extends to the lower side of the U-shaped plate, the lower side of the movable top plate is fixedly connected to the U-shaped connecting plate, the cam rotating rod is fitted under the U-shaped connecting plate, the rear side of the cam rotating rod passes through the cultivation device body and extends to the rear side of the cultivation device body, and the movable top plate is located below the disc.

[0017] Preferably, one side of the two first fixing plates is provided with an embedding groove, the embedding groove is adapted to the limiting sliding rod, and one side of the limiting sliding rod is clamped with the embedding groove through the embedding groove.

[0018] Beneficial effects of the present invention:

[0019] 1. The present invention can limit or release the placement plate by utilizing the movement and adjustment of the convex plate, and can lift up the placement plate by coordinating the upward movement of the movable top plate, which is beneficial for releasing the limits of all the placement plates at the same time, so that the placement plates can be directly taken or fixed later. The operation is simple and convenient, and there is no need to limit or release the limits one by one, which improves the flexibility and convenience of the single-cell cloning and cultivation device when used.

[0020] 2. The present invention uses a small gear to drive the large gear to rotate, so that the driven shaft can rotate smoothly, avoiding excessive rotation speed that affects single-cell cloning cultivation. As the driven shaft rotates, all the culture dishes in the disc can be rotated to the front position in turn, so that the cultivation status can be directly observed and recorded through the transparent glass window at the sealed door, without opening the sealed door, so that the temperature in the cultivation device is more stable.

[0021] 3. The present invention utilizes two elastic telescopic arc plates to fit and clamp the culture dish, and then utilizes an elastic limiting slide bar to position the placement plate, which is beneficial to the safe protection of the culture dish and prevents the culture dish from tipping over due to shaking. At the same time, the two sterilization lamps inside the cultivation device can perform sterilization and disinfection, effectively reducing the bacteria and viruses generated during cultivation, and ensuring a sterile environment for single-cell cloning cultivation. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings required for use in the embodiments or the prior art description are briefly introduced below. Obviously, for those skilled in the art, other drawings can be obtained based on these drawings without creative work.

[0023] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0024] Figure 2 yes Figure 1 Schematic diagram of the structure of the central sealing door and the transparent glass window;

[0025] Figure 3 yes Figure 1 Installation diagram of the driven gear and the driving gear rod;

[0026] Figure 4 yes Figure 3 A schematic diagram of the structure of the middle disc and the adapter slot;

[0027] Figure 5 yes Figure 4 The enlarged view of point A in the middle;

[0028] Figure 6 yes Figure 4 Schematic diagram of the structure of the middle disc and the placement plate;

[0029] Figure 7 yes Figure 6 Installation diagram of the middle U-shaped plate, movable top plate and spring 1;

[0030] Figure 8 yes Figure 1 The installation diagram of the first gear, the gear rotating rod and the chain;

[0031] Fig. 9 yes Figure 3 Schematic diagram of the structure of the electric push rod, extension plate and convex plate.

[0032] The reference numerals in the figures are as follows:

[0033] 1. Base; 2. U-shaped table; 3. Cultivation device body; 4. Driven shaft; 5. Disc; 6. Adapter slot; 7. Placement plate; 8. Culture dish; 9. U-shaped plate; 10. Active top plate; 11. Spring 1; 12. Cam rod; 13. U-shaped connecting plate; 14. First gear; 15. Chain; 16. Gear rod; 17. First servo motor; 18. First fixed plate; 19. Handle; 20. Second fixed plate; 21. Limiting slide rod; 22. Spring 2; 23. U-shaped seat; 24. Mounting seat; 25. Bidirectional threaded rod ; 26. Vertical plate; 27. Electric push rod; 28. Extension plate; 29. ​​Convex plate; 30. Second servo motor; 31. Bevel gear shaft; 32. Driven bevel gear; 33. Limiting round rod; 34. Third servo motor; 35. Hollow round groove; 36. Telescopic arc plate; 37. Spring three; 38. U-shaped handle; 39. Support plate; 40. Sealed door; 41. Transparent glass window; 42. Bracket; 43. Rubber rod; 44. Self-locking universal wheel; 45. Sterilization lamp; 46. Embedded groove; 111. Driven gear; 112. Driving gear rod. DETAILED DESCRIPTION

[0034] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] A single cell cloning culture device, such as Figure 1-9 As shown, it comprises a base 1, a U-shaped platform 2 is fixedly connected to the upper side of the base 1, a cultivation device body 3 is fixedly arranged on the upper side of the U-shaped platform 2, the cultivation device body 3 is provided with a two-way temperature control system for cooling and heating, and is used to flexibly control the temperature of the cultivation environment, a driven shaft 4 is rotatably installed inside the cultivation device body 3, a disk 5 is connected to the outer surface of the driven shaft 4 in an array, and an adapter slot 6 is opened on the upper side of the disk 5;

[0036] A placement plate 7 is clamped inside the adapter slot 6, and a culture dish 8 is fixed inside the placement plate 7. A U-shaped plate 9 distributed in an array is fixedly connected to one side of the interior of the cultivation device body 3. Two symmetrically arranged movable top plates 10 are slidably connected to the upper side of the U-shaped plate 9. A spring 11 is fixedly installed between the two movable top plates 10 and the U-shaped plate 9. A cam rotating rod 12 distributed in an array is rotatably connected to one side of the interior of the cultivation device body 3. The rear side of the cam rotating rod 12 is rotatably connected to a first gear 14, and the rear side of the cultivation device body 3 is rotatably connected to a gear rotating rod 16.

[0037] A chain 15 is sleeved between the first gear 14 and the gear rotating rod 16. The rear side of the gear rotating rod 16 is fixedly connected to the first servo motor 17 through a motor mounting plate. Two symmetrically arranged hollow circular grooves 35 are opened inside the disc 5. Four self-locking universal wheels 44 are fixedly connected to the lower side of the base 1. Two symmetrically arranged sterilization lamps 45 are fixedly arranged inside the cultivation device body 3. The sterilization lamp 45 usually uses a low-pressure mercury lamp. By energizing, the mercury atoms in the lamp tube are excited to generate ultraviolet rays to achieve a sterilization effect.

[0038] A first fixing plate 18 is fixedly installed on the upper side of the placement plate 7, and a handle 19 is fixedly provided on the upper side of the two first fixing plates 18. Two symmetrically arranged second fixing plates 20 are fixedly connected to the upper side of the disc 5. A limiting slide bar 21 for fixing the first fixing plate 18 is slidably installed on one side of the two second fixing plates 20. A spring 22 is fixedly connected between the two limiting slide bars 21 and the second fixing plates 20. One side of the limiting slide bar 21 passes through the second fixing plate 20 and extends to the other side of the second fixing plate 20. A U-shaped seat 23 is fixedly provided on one side of the two limiting slide bars 21.

[0039] A mounting seat 24 is fixedly installed at the bottom end of the main body 3 of the cultivation device, and a bidirectional threaded rotating rod 25 is rotatably installed on the inner side of the mounting seat 24. The outer surface of the bidirectional threaded rotating rod 25 is threadedly connected with two symmetrically arranged vertical plates 26. An electric push rod 27 is fixedly installed on one side of the two vertical plates 26. An extension plate 28 is fixedly installed on one side of the two electric push rods 27. One side of the two extension plates 28 is connected with a convex plate 29 in an array.

[0040] A bevel gear shaft 31 is rotatably connected to one side of the cultivation device body 3, and the right side of the two-way threaded rotating rod 25 passes through the mounting seat 24 and extends to the right side of the mounting seat 24. A driven bevel gear 32 is fixedly connected to the right side of the two-way threaded rotating rod 25. The bevel gear shaft 31 and the driven bevel gear 32 mesh with each other, and the meshing method is bevel gear meshing. Two limiting round rods 33 are fixedly connected to the inner side of the mounting seat 24, and the outer surfaces of the two limiting round rods 33 are slidably connected to the inside of the vertical plate 26. The two limiting round rods 33 can be used to guide the moving trajectory of the two vertical plates 26, so that the two vertical plates 26 can move back and forth smoothly, which is convenient to use. The rear side of the bevel gear shaft 31 passes through the cultivation device body 3 and extends to the rear side of the cultivation device body 3. The rear side of the bevel gear shaft 31 is fixedly connected to the third servo motor 34 through the motor mounting plate.

[0041] The placement plate 7 is internally slidably connected to an array of telescopic arc plates 36, and a spring 37 is fixedly connected between the telescopic arc plate 36 and the placement plate 7. The telescopic arc plate 36 is used to clamp the culture dish 8. Two U-shaped handles 38 are fixedly installed on the upper side of the placement plate 7. By installing the handle 19 and the U-shaped handle 38, the entire placement plate 7 can be lifted by both, which is used to move the culture dish 8 in the placement plate 7.

[0042] A driven gear 111 is fixedly connected to the outer surface of the driven rotating shaft 4, and a driving gear rod 112 is rotatably installed at the bottom end of the inner part of the cultivation device main body 3. The driving gear rod 112 and the driven gear 111 are meshed with each other. The lower side of the driving gear rod 112 penetrates the cultivation device main body 3 and extends to the lower side of the U-shaped platform 2. The lower side of the driving gear rod 112 is fixedly connected to the second servo motor 30 through the motor mounting plate.

[0043] The front side of the cultivation device body 3 is fixedly connected to two support plates 39, a sealed door 40 is rotatably connected between the two support plates 39, a transparent glass window 41 is fixedly provided inside the sealed door 40, and the rear side of the U-shaped platform 2 is fixedly connected to two brackets 42, a rubber rod 43 is fixedly installed between the two brackets 42, and the rubber rod 43 and the self-locking universal wheel 44 can be used to push the entire cultivation device body 3 to move, so as to save effort in moving the cultivation device.

[0044] The lower side of the movable top plate 10 passes through the U-shaped plate 9 and extends to the lower side of the U-shaped plate 9. The lower side of the movable top plate 10 is fixedly connected with a U-shaped connecting plate 13. The cam rotating rod 12 fits under the U-shaped connecting plate 13. When the top of the cam rotating rod 12 pushes up the U-shaped connecting plate 13, it is used to drive the elastic movable top plate 10 to move upward so as to push out the placement plate 7 in the disc 5, so as to conveniently take out the culture dish 8 in the placement plate 7. It is easy to use. The rear side of the cam rotating rod 12 passes through the cultivation device body 3 and extends to the rear side of the cultivation device body 3. The movable top plate 10 is located below the disc 5.

[0045] An embedding groove 46 is formed on one side of the two first fixing plates 18 . The embedding groove 46 is matched with the limiting sliding rod 21 . One side of the limiting sliding rod 21 is clamped with the embedding groove 46 through the embedding groove 46 .

[0046] When the present invention is in use, the placement plate 7 with the culture dish 8 is clamped into the adapter slot 6 of the disc 5, and then the two electric push rods 27 drive the two extension plates 28 to move backward, so that the convex plates 29 at the two extension plates 28 can be away from the inside of the two U-shaped seats 23, so as to release the limit of the limiting slide bar 21 installed with the spring 22, so that the limiting slide bar 21 with elastic force can be clamped into the embedding groove 46 of the second fixed plate 20, so as to further position the placement plate 7, ensure the stability of the placement plate 7, and make the culture dish 8 in the placement plate 7 more stable and safe to store. Then, when the placement plate 7 is to be taken out, the output shaft of the third servo motor 34 can drive the bevel gear shaft 31 to rotate, so that the bevel gear shaft 31 can drive the meshing two-way threaded rotating rod 25 to rotate continuously. At this time, the two vertical plates 26 on the two-way threaded rotating rod 25 can move toward each other to the two U-shaped seats 23, and then the two electric push rods 27 drive the two The convex plate 29 at the extension plate 28 enters the interior of the two U-shaped seats 23, and then the bidirectional threaded rotating rod 25 is reversed. In this way, as the two vertical plates 26 move relative to each other, the U-shaped seat 23 is pulled to move, so as to compress the limiting sliding rod 21 installed with the spring 22 to release the limit of the placement plate 7, and then the output shaft of the first servo motor 17 drives the gear rotating rod 16 to rotate, and then cooperates with the use of the chain 15, so that the gear rotating rod 16 can drive the five first gears 14 to rotate together. At this time, the cam rotating rod 12 can be slowly rotated, so that the top of the cam rotating rod 12 can lift the U-shaped connecting plate 13, so that the movable top plate 10 installed with the spring 11 can move upward and lift all the placement plates 7 inside the disc 5, which is conducive to simultaneously releasing the limit of the placement plates 7, so that the placement plates 7 can be directly taken or fixed later. The operation is simple and convenient, and there is no need to limit or release the limit one by one, which improves the flexibility and convenience of the single-cell cloning and cultivation device when used;

[0047] By utilizing the five disks 5 on the driven shaft 4, multiple culture dishes 8 can be classified and managed, and then the output shaft of the second servo motor 30 drives the driving gear rod 112 to rotate, so that the driving gear rod 112 can drive the meshing driven gear 111 to rotate. At this time, the small gear drives the large gear to rotate, so that the driven shaft 4 can rotate smoothly to avoid the influence of single cell cloning cultivation due to excessive rotation speed. With the rotation of the driven shaft 4, all the culture dishes 8 in the disk 5 can be rotated to the front position in turn, so that the cultivation status can be directly observed and recorded through the transparent glass window 41 at the sealed door 40, without opening the sealed door 40, so that the temperature in the cultivation device is more stable;

[0048] By placing the culture dish 8 into the placement plate 7, the culture dish 8 will squeeze the two telescopic arc plates 36 installed with the spring three 37, so that the two elastic telescopic arc plates 36 can fit and clamp the culture dish 8, which is beneficial to the safe protection of the culture dish 8 and prevent the culture dish 8 from tipping over due to shaking. Then the adapter slot 6 in the disc 5 can initially clamp the placement plate 7, and the elastic limit slide bar 21 can further stabilize the clamping of the limit slide bar 21. At the same time, the two sterilization lamps 45 inside the cultivation device can perform sterilization and disinfection, effectively reducing the bacteria and viruses generated during cultivation, and ensuring a sterile environment for single-cell cloning cultivation.

[0049] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, and these changes and improvements all fall within the scope of the present invention to be protected.

Claims

1. A single cell cloning culture device, comprising a base (1), characterized in that: A U-shaped platform (2) is fixedly connected to the upper side of the base (1), a cultivation device body (3) is fixedly provided on the upper side of the U-shaped platform (2), a driven shaft (4) is rotatably mounted inside the cultivation device body (3), a circular disk (5) is connected to the outer surface of the driven shaft (4) in an array, and an adapter slot (6) is provided on the upper side of the circular disk (5); A placement plate (7) is clamped inside the adapter slot (6), a culture dish (8) is fixedly arranged inside the placement plate (7), one side inside the cultivation device body (3) is fixedly connected to a U-shaped plate (9) distributed in an array, the upper side of the U-shaped plate (9) is slidably connected to two symmetrically arranged movable top plates (10), a spring (11) is fixedly installed between the two movable top plates (10) and the U-shaped plate (9), one side inside the cultivation device body (3) is rotatably connected to a cam rotating rod (12) distributed in an array, the rear side of the cam rotating rod (12) is rotatably connected to a first gear (14), and the rear side of the cultivation device body (3) is rotatably connected to a gear rotating rod (16).

2. A single cell cloning and cultivation device according to claim 1, characterized in that: A chain (15) is sleeved between the first gear (14) and the gear rotating rod (16); the rear side of the gear rotating rod (16) is fixedly connected to a first servo motor (17) via a motor mounting plate; two symmetrically arranged hollow circular grooves (35) are provided inside the disc (5); four self-locking universal wheels (44) are fixedly connected to the lower side of the base (1); and two symmetrically arranged sterilizing lamps (45) are fixedly provided inside the cultivation device body (3).

3. The single cell cloning and cultivation device according to claim 1, characterized in that: A first fixing plate (18) is fixedly installed on the upper side of the placement plate (7), and a handle (19) is fixedly installed on the upper side of the two first fixing plates (18). Two symmetrically arranged second fixing plates (20) are fixedly connected to the upper side of the disc (5), and a limiting slide bar (21) for fixing the first fixing plate (18) is slidably installed on one side of the two second fixing plates (20). A spring 2 (22) is fixedly connected between the two limiting slide bars (21) and the second fixing plate (20), and one side of the limiting slide bar (21) passes through the second fixing plate (20) and extends to the other side of the second fixing plate (20), and one side of the two limiting slide bars (21) is fixedly provided with a U-shaped seat (23).

4. The single cell cloning culture device according to claim 1, characterized in that: A mounting seat (24) is fixedly installed at the bottom end of the main body (3) of the cultivation device, a bidirectional threaded rotating rod (25) is rotatably installed on the inner side surface of the mounting seat (24), and the outer surface of the bidirectional threaded rotating rod (25) is threadedly connected with two symmetrically arranged vertical plates (26), one side of the two vertical plates (26) is fixedly installed with an electric push rod (27), one side of the two electric push rods (27) is fixedly provided with an extension plate (28), and one side of the two extension plates (28) is connected with a convex plate (29) in an array.

5. The single cell cloning culture device according to claim 4, characterized in that: A bevel gear shaft (31) is rotatably connected to one side of the cultivation device body (3); the right side of the bidirectional threaded rotating rod (25) penetrates the mounting seat (24) and extends to the right side of the mounting seat (24); a driven bevel gear (32) is fixedly connected to the right side of the bidirectional threaded rotating rod (25); the bevel gear shaft (31) and the driven bevel gear (32) are meshed with each other, and the meshing mode is bevel gear meshing; two limiting round rods (33) are fixedly connected to the inner side surface of the mounting seat (24); the outer surfaces of the two limiting round rods (33) are slidably connected to the inside of the vertical plate (26); the rear side of the bevel gear shaft (31) penetrates the cultivation device body (3) and extends to the rear side of the cultivation device body (3); the rear side of the bevel gear shaft (31) is fixedly connected to a third servo motor (34) through a motor mounting plate.

6. The single cell cloning culture device according to claim 1, characterized in that: The placement plate (7) is internally slidably connected to an array of telescopic arc plates (36), a spring three (37) is fixedly connected between the telescopic arc plate (36) and the placement plate (7), the telescopic arc plate (36) is used to clamp the culture dish (8), and two U-shaped handles (38) are fixedly installed on the upper side of the placement plate (7).

7. The single cell cloning culture device according to claim 1, characterized in that: The outer surface of the driven rotating shaft (4) is fixedly connected with a driven gear (111); the bottom end of the cultivation device body (3) is rotatably mounted with a driving gear rod (112); the driving gear rod (112) and the driven gear (111) are meshed with each other; the lower side of the driving gear rod (112) penetrates the cultivation device body (3) and extends to the lower side of the U-shaped platform (2); the lower side of the driving gear rod (112) is fixedly connected with a second servo motor (30) via a motor mounting plate.

8. The single cell cloning and cultivation device according to claim 1, characterized in that: The front side of the cultivation device body (3) is fixedly connected to two support plates (39), a sealed door (40) is rotatably connected between the two support plates (39), a transparent glass window (41) is fixedly provided inside the sealed door (40), and the rear side of the U-shaped platform (2) is fixedly connected to two brackets (42), and a rubber rod (43) is fixedly installed between the two brackets (42).

9. The single cell cloning culture device according to claim 1, characterized in that: The lower side of the movable top plate (10) passes through the U-shaped plate (9) and extends to the lower side of the U-shaped plate (9); the lower side of the movable top plate (10) is fixedly connected to a U-shaped connecting plate (13); the cam rotating rod (12) is fitted under the U-shaped connecting plate (13); the rear side of the cam rotating rod (12) passes through the cultivation device body (3) and extends to the rear side of the cultivation device body (3); and the movable top plate (10) is located below the disc (5).

10. The single cell cloning culture device according to claim 3, characterized in that: One side of each of the two first fixing plates (18) is provided with an embedding groove (46), the embedding groove (46) is adapted to the limiting slide bar (21), and one side of the limiting slide bar (21) is clamped with the embedding groove (46) via the embedding groove (46).

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