Composition containing cysteine, gibberellic acid, 6-benzylamino adenine and 28-homobrassinolide and application thereof
By using compositions containing cysteine, gibberellic acid, 6-benzylaminoadenine and 28-high brassinolide, the problems of frost damage and growth of tomato seedlings are solved, and the effects of alleviating frost damage, promoting growth and improving quality are achieved.
Patent Information
- Application Number
- CN202411890929.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-20
- Publication Date
- 2025-04-29
AI Technical Summary
The prior art is difficult to effectively alleviate the frost damage of tomato seedlings, while promoting the growth and improving quality of tomato seedlings.
Using a composition containing cysteine, gibberellic acid, 6-benzylaminoadenine and 28-high brassinolide, by regulating nutrient delivery and supplementing nutrients, reduces frost damage and promotes the growth of tomato seedlings.
While alleviating the freezing damage of tomato seedlings, it significantly promotes the growth of tomato seedlings, improves quality, and improves the resistance of tomato seedlings to adversity.
Smart Images
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Abstract
Description
Technical Field
[0001] The invention relates to a plant growth regulator, in particular to a composition containing cysteine, gibberellic acid, 6-benzylaminoadenine and 28-homobrassinolide and application thereof in alleviating frost damage of tomato seedlings. Background Art
[0002] 6-Benzylaminoadenine (6-BA) is a synthetic cytokinin that inhibits the decomposition of chlorophyll, nucleic acid, and protein in plant leaves, keeps them green and prevents aging; it can transport amino acids, auxins, inorganic salts, etc. to the treated parts, and has many other functions. It is widely used in agriculture, fruit trees, and horticultural crops from germination to harvest. The original drug of 6-benzylaminopurine is white crystals, insoluble in water, slightly soluble in ethanol, and stable in acid and alkali. At present, 6-BA is commonly used to preserve vegetables. In tissue culture work, cytokinins are indispensable additional hormones in differentiation culture media; 6-BA can also be used on fruit trees, mainly to promote cell expansion, increase fruit setting rate, and delay leaf aging; 6-BA can carry out cell division on stem tips, root tips, immature seeds, germinating seeds, growing fruits, etc.
[0003] Brassinolide is a sterol compound with plant growth regulating effect. Its chemical structure is homologous to many animal hormones, such as androgens, adrenocortical hormones, estrogens, insect ecdysone, etc. This is a major feature of brassinolide that is different from other plant hormones. Most plant growth substances only have a regulatory effect on one or several physiological processes of plants. For example, gibberellins mainly promote the growth of plant organs, ethephon can promote plant maturity, and chlormequat and mepiquat can only be used to inhibit sudden growth. Brassinolide can play a regulatory role in all growth and development stages of plants, which is the most important feature that distinguishes brassinolide from other plant hormones. In actual use, brassinolide has a certain suitable concentration. If the concentration is too high, it will not only cause waste, but also may inhibit crops to varying degrees. 28-homobrassinolide is one of the brassinolide series products. It also exists naturally in plants and is also a type of chemical synthesis that is widely used.
[0004] The effects of brassinolide include: (1) promoting cell division and fruit enlargement, significantly promoting cell division, and promoting the lateral and longitudinal growth of organs, thereby enlarging the fruit; (2) delaying leaf senescence, prolonging the green period, strengthening chlorophyll synthesis, improving photosynthesis, and causing the leaves to darken and turn green; (3) breaking apical dominance, promoting lateral bud germination, inducing bud differentiation, promoting lateral branch formation, increasing the number of branches, increasing the number of flowers, and improving pollen fertilization, thereby increasing the number of fruits and yields; (4) improving crop quality and commercial quality. Inducing parthenocarpy, stimulating ovary enlargement, preventing flower and fruit drop, promoting protein synthesis, and increasing sugar content.
[0005] With the development of diversified demand, a variety of 28-homobrassinolide compound products have gradually emerged in the market in recent years, such as 28-homobrassinolide + 920, 28-homobrassinolide + thiadiol, 28-homobrassinolide + chlorfenapyr, 28-homobrassinolide + chlorfenapyr / methylpiperidin / chlormequat, etc. 28-homobrassinolide compound products have the advantages of a wide bactericidal spectrum, synergistic enhancement, and strong rapidity. They are highly favored by the market and have greatly expanded the market space for 28-homobrassinolide. However, these compounds are all intended to enhance the effect of 28-homobrassinolide in various growth and development stages of plants, thereby increasing yields. Once frost damage occurs during plant growth, the functions of the above compounds will be significantly weakened.
[0006] In cold seasons, frost damage to plants is quite common. In addition, 6-BA has poor mobility and is not effective when sprayed on leaves alone. Therefore, a product that can alleviate frost damage to plants is needed to solve this problem. Summary of the invention
[0007] In view of the problems existing in the prior art, the present invention provides a regulator containing cysteine, gibberellic acid, 6-benzylaminoadenine and 28-homobrassinolide, which can alleviate the frost damage of tomato seedlings and promote the growth of tomato seedlings. It has the dual function of nutrition regulation. When the tomato seedlings are frozen, it can regulate nutrient delivery, supplement nutrition, and alleviate the frost damage. While alleviating the frost damage, it can also significantly promote the growth of tomato seedlings, improve the quality, and enhance the ability of tomato seedlings to resist adversity.
[0008] On the one hand, the present invention provides a composition containing cysteine, gibberellic acid, 6-benzylaminoadenine and 28-homobrassinolide, characterized in that its active ingredients are cysteine, gibberellic acid, 6-benzylaminoadenine and 28-homobrassinolide, the content of cysteine is 1-2wt%, the content of gibberellic acid is 1-3wt%, the content of 6-benzylaminoadenine is 0.5-3wt%, and the content of 28-homobrassinolide is 0.005-0.02wt%.
[0009] The composition is a soluble solution, comprising the following components in percentage by weight:
[0010]
[0011] The composition is a suspension, comprising the following components in percentage by weight:
[0012]
[0013] The composition for alleviating frost damage to tomato seedlings preferably comprises the following components in percentage by weight:
[0014]
[0015]
[0016] The composition for alleviating frost damage to tomato seedlings preferably comprises the following components in percentage by weight:
[0017]
[0018] The mass ratio of cysteine, gibberellic acid, 6-benzylaminoadenine and 28-homobrassinolide is 1:2:1:0.005.
[0019] In another aspect, the present invention provides a method for preparing a composition containing cysteine, gibberellic acid, 6-benzylaminoadenine and 28-homobrassinolide, wherein the method comprises:
[0020] When the composition is a soluble liquid: first dissolve 28-homobrassinolide and gibberellic acid with ethanol, then add 6-benzylaminoadenine and N-methylpyrrolidone, stir until completely dissolved, then add N-dodecyldimethylamine and continue to stir evenly to form solution 1; add cysteine into water and dissolve it, then add it to the previously stirred solution 1 and mix evenly to form a soluble liquid.
[0021] When the composition is a suspension agent, cysteine, gibberellic acid, 6-benzylaminoadenine, 28-homobrassinolide, sodium methylenebisnaphthalene sulfonate, aluminum oxide, silicon dioxide, N-dodecyldimethylamine and montmorillonite are mixed according to a proportion. Among them, aluminum oxide, silicon dioxide and montmorillonite are used as auxiliary materials, and cysteine, gibberellic acid, 6-benzylaminoadenine, 28-homobrassinolide and sodium methylenebisnaphthalene sulfonate are effective ingredients of medicines. After the components are mixed evenly, water is added, and then they are crushed and ground to 200-300 meshes to obtain the suspension agent composition of the present invention.
[0022] On the other hand, the present invention provides a composition containing cysteine, gibberellic acid, 6-benzylaminoadenine and 28-homobrassinolide for use when tomato seedlings are frozen. The composition is diluted 3000-5000 times with water and sprayed on the tomato seedlings at the early stage of the freezing damage.
[0023] The composition is sprayed twice on tomato seedlings, with an interval of 10-15 days between the two sprayings. DETAILED DESCRIPTION
[0024] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
[0025] All publications, patent applications, patents, and other references mentioned herein are incorporated herein by reference in their entirety. In the event of a conflict, the present specification (including definitions) shall prevail. In addition, the materials, methods, and examples described herein are illustrative only and are not intended to be limiting.
[0026] The terms "about" and "approximately" when used in conjunction with a numerical variable generally mean that the value of the variable and all values of the variable are within measurement or experimental error (e.g., 95% confidence interval for the mean) or within a wider range of the specified value (e.g., ±5% or ±10%).
[0027] The term "comprise" or its variations such as "contains", "has", "includes" means including the stated steps or elements but not excluding any other steps or elements. "Consisting of" means not excluding steps or elements not listed. "Consisting essentially of" means not excluding steps or elements that do not substantially affect the basic and novel features of the invention being protected. The term "comprising" specific steps or elements and its variations also include "consisting of" and "consisting essentially of" specific steps or elements.
[0028] When a numerical range is mentioned, it should be deemed to specifically disclose the specific values of its upper and lower limits, as well as all intermediate ranges included therein, such as the intermediate range between its upper or lower limit and any intermediate value, or the intermediate range between any two intermediate values thereof. In addition, any intermediate range, subrange and all individual values described in the numerical range can be excluded from the numerical range described.
[0029] The term "and / or" should be understood to refer to any one element or any combination of several elements connected by the term.
[0030] Crop frost damage index table:
[0031] Frost damage level Criteria 0 The plant is intact and has no damage symptoms. 1 Young leaves and less than 1 / 5 of the top leaves are frozen, but they can still recover and the yield reduction is within 10% 2 1 / 5-1 / 2 of the upper leaves were frozen and wilted, and some flower buds were frostbitten 3 More than 1 / 2 of the upper leaves are frozen and dead, and a large number of flower buds fall off 4 Most of the leaves died and most of the flower buds were frozen.
[0032] In some embodiments, the content of cysteine is 1-2wt%, for example, the mass content of cysteine can be 1wt%, 2wt%; the content of gibberellic acid is 1-3wt%, for example, the mass content of gibberellic acid can be 1wt%, 2wt%, 3wt%; the content of 6-benzylaminoadenine is 0.5-3wt%, for example, the mass content of 6-benzylaminoadenine can be 0.5wt%, 0.6wt%, 0.7wt%, 0.8wt%, 0.9wt%, 1.0wt% t%, 1.5wt%, 2.0wt%, 2.5wt%, 3.0wt%; the content of 28-homobrassinolide is 0.005-0.02wt%, for example, the mass content of 28-homobrassinolide can be 0.005wt%, 0.006wt%, 0.007wt%, 0.008wt%, 0.009wt%, 0.010wt%, 0.012wt%, 0.014wt%, 0.016wt%, 0.018wt%, 0.02wt%.
[0033] In some embodiments, when the composition is a soluble solution, it preferably includes the following components in weight percentages:
[0034]
[0035]
[0036] In some embodiments, when the composition is a soluble solution, the weight percentage of each substance is as follows:
[0037]
[0038] In some embodiments, the composition is a suspension, and the weight percentage of each substance is as follows:
[0039]
[0040] In some embodiments, when the composition is a suspension, the weight percentage of each substance is as follows:
[0041]
[0042]
[0043] In some embodiments, the mass ratio of cysteine, gibberellic acid, 6-benzylaminoadenine and 28-homobrassinolide is 1:2:1:0.005.
[0044] The term "soluble concentrate" used in this specification refers to a homogeneous liquid preparation in which the active ingredient forms a true solution after dilution with water. This formulation is generally composed of an active ingredient, an organic solvent and an appropriate adjuvant. Its active ingredient has a certain water solubility or a strong polarity, and its water dilution forms a true solution within a certain concentration range. If the dilution concentration exceeds its solubility range, it is easy to precipitate crystals. Compared with emulsifiable concentrates, the amount of organic solvent used is small, the environmental pollution is less, and the drug effect is also very good. It is the trend of future pesticide formulation development.
[0045] The term "suspension concentrate" used in this specification is a pesticide formulation that uses water as a medium and is processed by wet ultrafine grinding of pesticide raw materials and adjuvants. It has some characteristics of both emulsifiable concentrates and wettable powders, without the flammability and pesticide damage problems caused by organic solvents. The main components of pesticide suspensions include pesticide raw materials, various fillers and media (water), and the multiphase dispersion system formed allows the solid raw materials to be evenly dispersed in water with a certain degree of dispersion. This formulation avoids the pollution of the environment caused by flying dust and the harm to the human body. At the same time, compared with emulsifiable concentrate formulations, it reduces the use of organic solvents, which is beneficial to environmental protection and saves petroleum resources. The advantages of pesticide suspensions are their high storage stability, which can effectively prevent the decomposition or ineffectiveness of the active ingredients; some dosage forms can control the release rate of the active ingredients of the original drug, extend the effective period, and reduce the number and amount of applications; after high-concentration original drugs are processed and diluted, the suspension performance is enhanced, and its active ingredients can be evenly distributed, which can achieve a better dispersion effect when applied; high-concentration original drugs are made into low-toxic preparations, which are safe to use and reduce environmental pollution; they are conducive to the penetration of active ingredients and absorption by target organisms, thereby improving the efficacy.
[0046] The present invention is further described by the following examples, which should not be construed as limiting the present invention.
[0047] Unless otherwise specified, all reagents used in the following examples are commercially available products.
[0048] Example 1
[0049] Components and mass content: 1% cysteine, 1% gibberellic acid, 0.5% 6-benzylaminoadenine, 0.005% 28-homobrassinolide, 40% ethanol, 5% N-methylpyrrolidone, 15% N-dodecyldimethylamine, and the balance is water (solvent). The above ingredients are weighed by weight, dissolved and mixed to prepare an aqueous solution, and the above raw materials are all commercially available qualified products.
[0050] Preparation method: first dissolve 28-homobrassinolide and gibberellic acid with ethanol, then add 6-benzylaminoadenine and N-methylpyrrolidone, stir until completely dissolved, then add N-dodecyldimethylamine and continue stirring to form solution 1; add cysteine into water and dissolve it, then add it to the previously stirred solution 1 and mix well to form a soluble solution.
[0051] Example 2
[0052] Components and mass content: 1% cysteine, 2% gibberellic acid, 1% 6-benzylaminoadenine, 0.008% 28-homobrassinolide, 40% ethanol, 10% N-methylpyrrolidone, 20% N-dodecyldimethylamine, and the balance is water (solvent). The above ingredients are weighed by weight, dissolved and mixed to prepare an aqueous solution, and the above raw materials are all commercially available qualified products.
[0053] Preparation method: first dissolve 28-homobrassinolide and gibberellic acid with ethanol, then add 6-benzylaminoadenine and N-methylpyrrolidone, stir until completely dissolved, then add N-dodecyldimethylamine and continue stirring to form solution 1; add cysteine into water and dissolve it, then add it to the previously stirred solution 1 and mix well to form a soluble solution.
[0054] Example 3
[0055] Components and mass content: 1% cysteine, 3% gibberellic acid, 1% 6-benzylaminoadenine, 0.005% 28-homobrassinolide, 40% ethanol, 15% N-methylpyrrolidone, 20% N-dodecyldimethylamine, and the balance is water (solvent). The above ingredients are weighed by weight, dissolved and mixed to prepare an aqueous solution, and the above raw materials are all commercially available qualified products.
[0056] Preparation method: first dissolve 28-homobrassinolide and gibberellic acid with ethanol, then add 6-benzylaminoadenine and N-methylpyrrolidone, stir until completely dissolved, then add N-dodecyldimethylamine and continue stirring to form solution 1; add cysteine into water and dissolve it, then add it to the previously stirred solution 1 and mix well to form a soluble solution.
[0057] Example 4
[0058] Components and mass content: 2% cysteine, 2% gibberellic acid, 1% 6-benzylaminoadenine, 0.01% 28-homobrassinolide, 40% ethanol, 15% N-methylpyrrolidone, 20% N-dodecyldimethylamine, and the balance is water (solvent). The above ingredients are weighed by weight, dissolved and mixed to prepare an aqueous solution, and the above raw materials are all commercially available qualified products.
[0059] Preparation method: first dissolve 28-homobrassinolide and gibberellic acid with ethanol, then add 6-benzylaminoadenine and N-methylpyrrolidone, stir until completely dissolved, then add N-dodecyldimethylamine and continue stirring to form solution 1; add cysteine into water and dissolve it, then add it to the previously stirred solution 1 and mix well to form a soluble solution.
[0060] Example 5
[0061] Components and mass content: 2% cysteine, 4% gibberellic acid, 1% 6-benzylaminoadenine, 0.01% 28-homobrassinolide, 50% ethanol, 10% N-methylpyrrolidone, 25% N-dodecyldimethylamine, and the balance is water (solvent). The above ingredients are weighed by weight, dissolved and mixed to prepare an aqueous solution, and the above raw materials are all commercially available qualified products.
[0062] Preparation method: first dissolve 28-homobrassinolide and gibberellic acid with ethanol, then add 6-benzylaminoadenine and N-methylpyrrolidone, stir until completely dissolved, then add N-dodecyldimethylamine and continue stirring to form solution 1; add cysteine into water and dissolve it, then add it to the previously stirred solution 1 and mix well to form a soluble solution.
[0063] Example 6
[0064] Components and mass content: 2% cysteine, 3% gibberellic acid, 0.5% 6-benzylaminoadenine, 0.008% 28-homobrassinolide, 30% ethanol, 10% N-methylpyrrolidone, 20% N-dodecyldimethylamine, and the balance is water (solvent). The above ingredients are weighed by weight, dissolved and mixed to prepare an aqueous solution, and the above raw materials are all commercially available qualified products.
[0065] Preparation method: first dissolve 28-homobrassinolide and gibberellic acid with ethanol, then add 6-benzylaminoadenine and N-methylpyrrolidone, stir until completely dissolved, then add N-dodecyldimethylamine and continue stirring to form solution 1; add cysteine into water and dissolve it, then add it to the previously stirred solution 1 and mix well to form a soluble solution.
[0066] Example 7
[0067] Components and mass content: 2% cysteine, 3% gibberellic acid, 0.5% 6-benzylaminoadenine, 0.01% 28-homobrassinolide, 45% ethanol, 15% N-methylpyrrolidone, 15% N-dodecyldimethylamine, and the balance is water (solvent). The above ingredients are weighed by weight, dissolved and mixed to prepare an aqueous solution, and the above raw materials are all commercially available qualified products.
[0068] Preparation method: first dissolve 28-homobrassinolide and gibberellic acid with ethanol, then add 6-benzylaminoadenine and N-methylpyrrolidone, stir until completely dissolved, then add N-dodecyldimethylamine and continue stirring to form solution 1; add cysteine into water and dissolve it, then add it to the previously stirred solution 1 and mix well to form a soluble solution.
[0069] Example 8
[0070] Components and mass content: 2% cysteine, 3% gibberellic acid, 1.5% 6-benzylaminoadenine, 0.02% 28-homobrassinolide, 45% ethanol, 10% N-methylpyrrolidone, 30% N-dodecyldimethylamine, and the balance is water (solvent). The above ingredients are weighed by weight, dissolved and mixed to prepare an aqueous solution, and the above raw materials are all commercially available qualified products.
[0071] Preparation method: first dissolve 28-homobrassinolide and gibberellic acid with ethanol, then add 6-benzylaminoadenine and N-methylpyrrolidone, stir until completely dissolved, then add N-dodecyldimethylamine and continue stirring to form solution 1; add cysteine into water and dissolve it, then add it to the previously stirred solution 1 and mix well to form a soluble solution.
[0072] Example 9
[0073] Components and mass content: 3% cysteine, 2% gibberellic acid, 1.5% 6-benzylaminoadenine, 0.02% 28-homobrassinolide, 40% ethanol, 10% N-methylpyrrolidone, 20% N-dodecyldimethylamine, and the balance is water (solvent). The above ingredients are weighed by weight, dissolved and mixed to prepare an aqueous solution, and the above raw materials are all commercially available qualified products.
[0074] Preparation method: first dissolve 28-homobrassinolide and gibberellic acid with ethanol, then add 6-benzylaminoadenine and N-methylpyrrolidone, stir until completely dissolved, then add N-dodecyldimethylamine and continue stirring to form solution 1; add cysteine into water and dissolve it, then add it to the previously stirred solution 1 and mix well to form a soluble solution.
[0075] Example 10
[0076] Components and mass content: 1% cysteine, 1% gibberellic acid, 0.5% 6-benzylaminoadenine, 0.02% 28-homobrassinolide, 10% N-dodecyldimethylamine, 5% sodium methylenebisnaphthalenesulfonate, 10% alumina, 5% silicon dioxide, 10% montmorillonite, and the balance is water (solvent). The above ingredients are weighed by weight and are all commercially available qualified products.
[0077] Preparation method: cysteine, gibberellic acid, 6-benzylaminoadenine, 28-homobrassinolide, sodium methylenebisnaphthalenesulfonate, aluminum oxide, silicon dioxide, N-dodecyldimethylamine and montmorillonite are mixed according to a proportion; after the components are evenly mixed, water is added, and then they are crushed and ground to 200-300 meshes to obtain the suspension composition of the present invention.
[0078] Embodiment 11
[0079] Components and mass content: 1% cysteine, 2% gibberellic acid, 1% 6-benzylaminoadenine, 0.005% 28-homobrassinolide, 15% N-dodecyldimethylamine, 3% sodium methylenebisnaphthalenesulfonate, 15% alumina, 5% silicon dioxide, 15% montmorillonite, and the balance is water (solvent). The above ingredients are weighed by weight and are all commercially available qualified products.
[0080] Preparation method: cysteine, gibberellic acid, 6-benzylaminoadenine, 28-homobrassinolide, sodium methylenebisnaphthalenesulfonate, aluminum oxide, silicon dioxide, N-dodecyldimethylamine and montmorillonite are mixed according to a proportion; after the components are evenly mixed, water is added, and then they are crushed and ground to 200-300 meshes to obtain the suspension composition of the present invention.
[0081] Example 12
[0082] Components and mass content: 1% cysteine, 2% gibberellic acid, 1% 6-benzylaminoadenine, 0.02% 28-homobrassinolide, 20% N-dodecyldimethylamine, 1% sodium methylenebisnaphthalenesulfonate, 20% alumina, 10% silicon dioxide, 10% montmorillonite, and the balance is water (solvent). The above ingredients are weighed by weight and are all commercially available qualified products.
[0083] Preparation method: cysteine, gibberellic acid, 6-benzylaminoadenine, 28-homobrassinolide, sodium methylenebisnaphthalenesulfonate, aluminum oxide, silicon dioxide, N-dodecyldimethylamine and montmorillonite are mixed according to a proportion; after the components are evenly mixed, water is added, and then they are crushed and ground to 200-300 meshes to obtain the suspension composition of the present invention.
[0084] Example 13
[0085] Components and mass content: 1% cysteine, 2% gibberellic acid, 1% 6-benzylaminoadenine, 0.01% 28-homobrassinolide, 10% N-dodecyldimethylamine, 5% sodium methylenebisnaphthalenesulfonate, 20% alumina, 10% silicon dioxide, 10% montmorillonite, and the balance is water (solvent). The above ingredients are weighed by weight and are all commercially available qualified products.
[0086] Preparation method: cysteine, gibberellic acid, 6-benzylaminoadenine, 28-homobrassinolide, sodium methylenebisnaphthalenesulfonate, aluminum oxide, silicon dioxide, N-dodecyldimethylamine and montmorillonite are mixed according to a proportion; after the components are evenly mixed, water is added, and then they are crushed and ground to 200-300 meshes to obtain the suspension composition of the present invention.
[0087] Embodiment 14
[0088] Components and mass content: 1% cysteine, 3% gibberellic acid, 2% 6-benzylaminoadenine, 0.01% 28-homobrassinolide, 20% N-dodecyldimethylamine, 5% sodium methylenebisnaphthalenesulfonate, 15% alumina, 10% silicon dioxide, 10% montmorillonite, and the balance is water (solvent). The above ingredients are weighed by weight and are all commercially available qualified products.
[0089] Preparation method: cysteine, gibberellic acid, 6-benzylaminoadenine, 28-homobrassinolide, sodium methylenebisnaphthalenesulfonate, aluminum oxide, silicon dioxide, N-dodecyldimethylamine and montmorillonite are mixed according to a proportion; after the components are evenly mixed, water is added, and then they are crushed and ground to 200-300 meshes to obtain the suspension composition of the present invention.
[0090] Embodiment 15
[0091] Components and mass content: 1% cysteine, 3% gibberellic acid, 3% 6-benzylaminoadenine, 0.02% 28-homobrassinolide, 15% N-dodecyldimethylamine, 5% sodium methylenebisnaphthalenesulfonate, 20% alumina, 10% silicon dioxide, 15% montmorillonite, and the balance is water (solvent). The above ingredients are weighed by weight and are all commercially available qualified products.
[0092] Preparation method: cysteine, gibberellic acid, 6-benzylaminoadenine, 28-homobrassinolide, sodium methylenebisnaphthalenesulfonate, aluminum oxide, silicon dioxide, N-dodecyldimethylamine and montmorillonite are mixed according to a proportion; after the components are evenly mixed, water is added, and then they are crushed and ground to 200-300 meshes to obtain the suspension composition of the present invention.
[0093] Example 16
[0094] Components and mass content: 2% cysteine, 1% gibberellic acid, 0.5% 6-benzylaminoadenine, 0.005% 28-homobrassinolide, 15% N-dodecyldimethylamine, 5% sodium methylenebisnaphthalenesulfonate, 20% alumina, 10% silicon dioxide, 15% montmorillonite, and the balance is water (solvent). The above ingredients are weighed by weight and are all commercially available qualified products.
[0095] Preparation method: cysteine, gibberellic acid, 6-benzylaminoadenine, 28-homobrassinolide, sodium methylenebisnaphthalenesulfonate, aluminum oxide, silicon dioxide, N-dodecyldimethylamine and montmorillonite are mixed according to a proportion; after the components are evenly mixed, water is added, and then they are crushed and ground to 200-300 meshes to obtain the suspension composition of the present invention.
[0096] Embodiment 17
[0097] Components and mass content: 2% cysteine, 2% gibberellic acid, 1% 6-benzylaminoadenine, 0.01% 28-homobrassinolide, 20% N-dodecyldimethylamine, 5% sodium methylenebisnaphthalenesulfonate, 10% alumina, 5% silicon dioxide, 15% montmorillonite, and the balance is water (solvent). The above ingredients are weighed by weight and are all commercially available qualified products.
[0098] Preparation method: cysteine, gibberellic acid, 6-benzylaminoadenine, 28-homobrassinolide, sodium methylenebisnaphthalenesulfonate, aluminum oxide, silicon dioxide, N-dodecyldimethylamine and montmorillonite are mixed according to a proportion; after the components are evenly mixed, water is added, and then they are crushed and ground to 200-300 meshes to obtain the suspension composition of the present invention.
[0099] Embodiment 18
[0100] Components and mass content: 2% cysteine, 3% gibberellic acid, 1% 6-benzylaminoadenine, 0.02% 28-homobrassinolide, 20% N-dodecyldimethylamine, 5% sodium methylenebisnaphthalenesulfonate, 10% alumina, 5% silicon dioxide, 15% montmorillonite, and the balance is water (solvent). The above ingredients are weighed by weight and are all commercially available qualified products.
[0101] Preparation method: cysteine, gibberellic acid, 6-benzylaminoadenine, 28-homobrassinolide, sodium methylenebisnaphthalenesulfonate, aluminum oxide, silicon dioxide, N-dodecyldimethylamine and montmorillonite are mixed according to a proportion; after the components are evenly mixed, water is added, and then they are crushed and ground to 200-300 meshes to obtain the suspension composition of the present invention.
[0102] Example effect test experiment
[0103] The composition containing cysteine, gibberellic acid, 6-benzylaminoadenine and 28-homobrassinolide obtained in Examples 1-18 was diluted to 3000 times, 4000 times and 5000 times, respectively. The control group was sprayed with the same amount of clean water. The spraying was performed once one day before the arrival of low temperature (outdoor temperature ≤ 0°C). After the low temperature, the degree of frostbite of tomato seedlings was investigated (see Table 1), and then sprayed twice in succession. The interval between the two applications was 10 days. The growth recovery of the tomato plants was observed 10 days after the second application (see Table 2).
[0104] Table 1: Test results on frost-damaged tomato seedlings
[0105]
[0106]
[0107] Table 2: Growth of tomato seedlings 10 days after the second application
[0108]
[0109]
[0110] It can be concluded from Table 1 and Table 2 that the composition can alleviate the freezing damage of tomato seedlings when the dilution multiple is between 3000-5000, but the effect of alleviating freezing damage is better when the dilution multiple is between 3000-4000. It can also be clearly concluded from the above table that when the weight percentage of the active ingredient 6-benzylaminoadenine is between 0.5-1% and the weight percentage of 28-homobrassinolide is between 0.005-0.01%, the composition has the best effect on alleviating freezing damage of tomato seedlings and promoting the growth of tomato seedlings.
[0111] The implementation of the present invention is not limited to the above-mentioned embodiments. Without departing from the spirit and scope of the present invention, ordinary technicians in this field can make various changes and improvements to the present invention in form and detail, and these are considered to fall within the protection scope of the present invention.
Claims
1. A composition containing cysteine, gibberellic acid, 6-benzylaminoadenine and 28-homobrassinolide, characterized in that: The invention takes cysteine, gibberellic acid, 6-benzylaminoadenine and 28-homobrassinolide as effective ingredients, wherein the content of cysteine is 1-2wt%, the content of gibberellic acid is 1-3wt%, the content of 6-benzylaminoadenine is 0.5-3wt%, and the content of 28-homobrassinolide is 0.005-0.02wt%.
2. The composition according to claim 1, characterized in that The mass ratio of cysteine, gibberellic acid, 6-benzylaminoadenine and 28-homobrassinolide is 1:2:1:0.
005.
3. The composition according to claim 1, characterized in that: The composition is a soluble solution, consisting of the following components in percentage by weight:
4. The composition according to claim 1, characterized in that: The composition is a suspension, which is composed of the following components in percentage by weight:
5. The composition according to claim 1, characterized in that: The composition is a soluble solution, consisting of the following components in percentage by weight:
6. The composition according to claim 1, characterized in that: The composition is a suspension, which is composed of the following components in percentage by weight:
7. The method for preparing the composition according to claim 3, characterized in that: First, dissolve 28-homobrassinolide and gibberellic acid with ethanol, then add 6-benzylaminoadenine and N-methylpyrrolidone, stir until completely dissolved, then add N-dodecyldimethylamine and continue stirring to form solution 1; add cysteine to water and dissolve it, then add it to the previously stirred solution 1 and mix well to form a soluble solution.
8. The method for preparing the composition according to claim 4, characterized in that: Cysteine, gibberellic acid, 6-benzylaminoadenine, 28-homobrassinolide, sodium methylenebisnaphthalenesulfonate, aluminum oxide, silicon dioxide, N-dodecyldimethylamine and montmorillonite are mixed according to a proportion; after the components are evenly mixed, water is added, and then they are crushed and ground to 200-300 meshes to obtain the suspension composition of the present invention.
9. Use of the composition containing cysteine, gibberellic acid, 6-benzylaminoadenine and 28-homobrassinolide according to any one of claims 1 to 6 in alleviating frost damage to tomato seedlings, characterized in that: The composition is diluted 3000-5000 times with water and sprayed on the tomato seedlings after the tomato seedlings are frozen.
10. The use of the composition according to claim 9, characterized in that: The composition containing cysteine, gibberellic acid, 6-benzylaminoadenine and 28-homobrassinolide is sprayed twice on tomato seedlings with frost damage, and the interval between the two sprayings is 10-15 days.