Application of royal jelly protein in medicine for treating Alzheimer's disease and preparation equipment of royal jelly protein

Abstract

The invention discloses application of royal jelly protein in a medicine for treating Alzheimer's disease. The application comprises the following steps: S1, extracting royal jelly protein; s2, preparing medicine raw materials; s3, mixing the raw materials: mixing the raw materials to obtain a mixed raw material medicine; and S4, adding water into the mixed raw material medicine, decocting twice, filtering after each time of decoction, combining the two filtrates, and concentrating under reduced pressure to obtain an extract, namely the royal jelly protein-containing medicine for treating the Alzheimer's disease. The invention further discloses preparation equipment of the royal jelly protein in the medicine for treating the Alzheimer's disease. The royal jelly protein is applied to the medicine for treating the Alzheimer's disease, and the royal jelly protein is rich in vitamin B and a large amount of protein, has a very strong bactericidal ability, has an effect of inhibiting the growth of cancer cells, can improve nutrition, supplement brain power and promote metabolism of a human body, and can be used for treating the Alzheimer's disease. Therefore, the effect of treating the Alzheimer's disease can be improved, and the occurrence probability of the Alzheimer's disease is reduced.

Description

Technical Field

[0001] The invention belongs to the field of pharmaceutical technology, and in particular relates to application of royal jelly protein in Alzheimer's disease drugs and a preparation device thereof. Background Art

[0002] The most common form of Alzheimer's disease is Alzheimer's disease, a progressive neurodegenerative disease with an insidious onset. Clinically, it is characterized by comprehensive dementia manifestations such as memory impairment, aphasia, apraxia, agnosia, visual-spatial skill impairment, executive dysfunction, and personality and behavioral changes. The cause of the disease is still unknown. With the advent of an aging society, more and more elderly people are suffering from Alzheimer's disease, and Alzheimer's disease has become one of the "epidemics" in the world today. The daily living ability of patients with Alzheimer's disease declines, bringing endless pain and troubles to themselves and the people around them. The average survival period of patients with Alzheimer's disease is 5.5 years, making the disease one of the main causes of death among the elderly in modern society. For this reason, the treatment of Alzheimer's disease is one of the important directions of the current research stage.

[0003] Recent studies have found that royal jelly protein can improve the body's resistance, promote the regeneration and repair of internal organs and tissues, adjust endocrine and metabolism, and effectively increase appetite, improve sleep and promote growth and development. It has a strong health care function and medical effect on the human body. Therefore, using royal jelly protein in drugs for the treatment of senile dementia can provide senile dementia patients with rich vitamin B and a large amount of protein, thereby improving nutrition and supplementing brain power, and has a certain therapeutic and preventive effect. For this reason, we propose the application of royal jelly protein in senile dementia drugs and its preparation equipment. Summary of the invention

[0004] The purpose of the present invention is to provide the application of royal jelly protein in Alzheimer's disease drugs and its preparation equipment to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solution: Application of royal jelly protein in Alzheimer's disease drugs, comprising the following steps:

[0006] S1. Extracting royal jelly protein: extracting royal jelly protein from royal jelly and setting aside;

[0007] S2. Preparation of drug raw materials: Prepare the following raw materials by weight: 10-30 parts of royal jelly protein, 5-15 parts of red peony root, 20-40 parts of astragalus, 20-40 parts of salvia miltiorrhiza, 20-40 parts of epimedium, 20-40 parts of polygala, 10-20 parts of chuanxiong, 10-20 parts of yizhiren, 10-20 parts of angelica, 10-20 parts of poria, 15-25 parts of polygonum multiflorum;

[0008] S3. Raw material mixing: mixing the above raw materials to obtain a mixed raw material drug;

[0009] S4. The mixed raw materials are decocted twice with water, the first time the amount of water added is 2-6 times the total weight of the mixed raw materials, and the decoction is performed for 60-80 minutes, and the second time the amount of water added is 1-4 times the total weight of the mixed raw materials, and the decoction is performed for 20-40 minutes. Filter after each decoction, combine the two filtrates, and concentrate under reduced pressure to obtain an extract, that is, a drug for treating Alzheimer's disease containing royal jelly protein.

[0010] Preferably, the preparation of the drug raw materials in S2: prepare the raw materials according to the following weight parts: 20 parts of royal jelly protein, 10 parts of red peony root, 30 parts of astragalus, 30 parts of salvia miltiorrhiza, 30 parts of epimedium, 30 parts of polygala, 15 parts of chuanxiong, 15 parts of alpinia oxyphylla, 15 parts of angelica, 15 parts of poria, and 20 parts of polygonum multiflorum.

[0011] Preferably, the specific steps of extracting royal jelly protein in S1 are:

[0012] S101. The buffer solution is prepared by potassium hydroxide, and then royal jelly is added into the buffer solution for mixing to obtain a mixed solution;

[0013] S102. The mixed solution obtained in S101 is filtered to remove large particles of impurities in the mixed solution to obtain a filtrate;

[0014] S103. The filtrate obtained in S102 is centrifuged, and during the centrifugation, the centrifuge is operated under a low temperature environment, and then the upper layer of the centrifuged solution is filtered;

[0015] S104. The centrifuged supernatant obtained in S103 is precipitated with royal jelly protein by PEG and antifreeze, and the solution is stirred at all times during the precipitation process by PEG and antifreeze;

[0016] S105. The royal jelly protein obtained in S104 is purified by ion exchange chromatography. The royal jelly protein is purified and concentrated by the mutual charge interaction between the royal jelly protein and the ion exchange resin to obtain the royal jelly protein.

[0017] Preferably, the buffer in S101 is Tris-HCl buffer, the concentration of the Tris-HCl buffer is 0.02-0.08 mol / L, and the pH is 7.3-7.9.

[0018] Preferably, after the raw materials are mixed in S3, the step further includes: removing impurities, washing, slicing and drying the raw materials accordingly.

[0019] The preparation device of royal jelly protein in Alzheimer's disease drugs comprises a fixed base, three groups of legs are welded on the upper surface of the fixed base, a mixing barrel is welded on the top of the three groups of legs, four groups of support rods are welded on the top of the mixing barrel, a cross plate is welded on the top of the four groups of support rods, a first servo motor is fixedly installed at the middle position of the upper surface of the cross plate, the output end of the first servo motor is connected to the first rotating shaft through a transmission, the bottom end of the first rotating shaft is connected to a connecting rod through a fixed component, a stirring rod is welded on the lower surface of the other end of the connecting rod, a liquid outlet is connected to the bottom of the mixing barrel, a liquid outlet valve is installed on the liquid outlet, a cushion seat is fixedly provided on the upper surface of the fixed base, a filter barrel is rotatably connected to the upper surface of the cushion seat through a driving component, a funnel-shaped filter screen is provided on the top of the filter barrel through a clamping component, a discharge port is connected to one side of the bottom of the filter barrel, a discharge valve is fixedly installed on the discharge port, and a guide plate is provided at the inner bottom of the filter barrel.

[0020] Preferably, the fixing assembly includes a protrusion and a fastening bolt, the protrusion is provided in two groups, the two groups of protrusions are welded to the outer surface of the connecting rod, and the two groups of protrusions are fixed on both sides of the first rotating shaft by the fastening bolts.

[0021] Preferably, the driving assembly includes a receiving groove, a second servo motor and a second rotating shaft, the receiving groove is arranged inside the pad, the second servo motor is installed in the receiving groove, the bottom end of the second rotating shaft is transmission connected to the output end of the second servo motor, and the top of the second rotating shaft is fixedly connected to the bottom of the filter barrel.

[0022] Preferably, a circle of balls is annularly arranged on the upper surface of the seat, the balls are rotatably connected to the seat, and the bottom of the filter barrel is arranged on the upper surface of the balls.

[0023] Preferably, the clamping assembly includes a fixing ring and a limiting ring, the fixing ring is fixedly arranged on the upper surface of the funnel-shaped filter screen, the limiting ring is fixedly arranged on the lower surface of the fixing ring, and the limiting ring is arranged on the outer surface of the filter barrel.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] (1) The present invention applies royal jelly protein to a drug for treating Alzheimer's disease. Royal jelly protein contains rich vitamin B and a large amount of protein, has a strong bactericidal ability, has the effect of inhibiting the growth of cancer cells, and can improve nutrition, supplement brain power and promote human metabolism, thereby improving the effect of treating Alzheimer's disease and reducing the probability of Alzheimer's disease.

[0026] (2) The present invention drives the first rotating shaft to rotate by the first servo motor, and the rotation of the first rotating shaft drives the stirring rod to rotate, so that the stirring rod mixes the materials inside the mixing barrel, thereby facilitating the mixing of the materials. At the same time, the fixing component can be used to facilitate the disassembly and cleaning of the stirring rod;

[0027] (3) The present invention sets a filter barrel and uses a funnel-shaped filter to filter the mixed liquid discharged from the liquid outlet, thereby facilitating the removal of large particles of impurities in the mixed liquid. At the same time, the eccentricity principle is used to make the outer side of the funnel-shaped filter located at the liquid outlet for receiving and filtering the material, so that the large particles of impurities fall to the center of the funnel-shaped filter, thereby increasing the filtering effect and reducing the possibility of clogging. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is one of the structural schematic diagrams of the present invention;

[0029] Figure 2 This is the second structural schematic diagram of the present invention;

[0030] Figure 3 For the present invention Figure 1 Enlarged view of point A in the middle;

[0031] Figure 4 It is a structural cross-sectional view of the cushion seat and the filter barrel part of the present invention.

[0032] In the figure: 1. fixed base; 2. support leg; 3. mixing barrel; 4. support rod; 5. cross plate; 6. first servo motor; 7. first rotating shaft; 8. connecting rod; 9. stirring rod; 10. liquid outlet; 11. liquid outlet valve; 12. cushion seat; 13. filter barrel; 14. funnel-shaped filter screen; 15. discharge port; 16. discharge valve; 17. guide plate; 18. bump; 19. fastening bolt; 20. receiving groove; 21. second servo motor; 22. second rotating shaft; 23. ball; 24. fixing ring; 25. limit ring. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] Example 1

[0035] The present invention provides a technical solution: application of royal jelly protein in Alzheimer's disease drugs, comprising the following steps:

[0036] S1. Extracting royal jelly protein: extracting royal jelly protein from royal jelly and setting aside;

[0037] S2. Preparation of drug raw materials: Prepare the following raw materials by weight: 10 parts of royal jelly protein, 5 parts of red peony root, 20 parts of astragalus, 20 parts of salvia miltiorrhiza, 20 parts of epimedium, 20 parts of polygala, 10 parts of chuanxiong, 10 parts of yizhiren, 10 parts of angelica, 10 parts of poria, 15 parts of polygonum multiflorum;

[0038] S3. Raw material mixing: mixing the above raw materials to obtain a mixed raw material drug;

[0039] S4. The mixed raw materials are decocted twice with water, the first time the amount of water added is 2 times the total weight of the mixed raw materials, and the decoction is performed for 60 minutes, and the second time the amount of water added is 1 times the total weight of the mixed raw materials, and the decoction is performed for 20 minutes. Filter after each decoction, combine the two filtrates, and concentrate under reduced pressure to obtain an extract, that is, a drug for treating Alzheimer's disease containing royal jelly protein.

[0040] In this embodiment, preferably, the specific steps of extracting royal jelly protein in S1 are:

[0041] S101. The buffer solution is prepared by potassium hydroxide, and then royal jelly is added into the buffer solution for mixing to obtain a mixed solution;

[0042] S102. The mixed solution obtained in S101 is filtered to remove large particles of impurities in the mixed solution to obtain a filtrate;

[0043] S103. The filtrate obtained in S102 is centrifuged, and during the centrifugation, the centrifuge is operated under a low temperature environment, and then the upper layer of the centrifuged solution is filtered;

[0044] S104. The centrifuged supernatant obtained in S103 is precipitated with royal jelly protein by PEG and antifreeze, and the solution is stirred at all times during the precipitation process by PEG and antifreeze;

[0045] S105. The royal jelly protein obtained in S104 is purified by ion exchange chromatography. The royal jelly protein is purified and concentrated by the mutual charge interaction between the royal jelly protein and the ion exchange resin to obtain the royal jelly protein.

[0046] In this embodiment, preferably, the buffer in S101 is Tris-HCl buffer, the concentration of the Tris-HCl buffer is 0.02 mol / L, and the pH is 7.3.

[0047] In this embodiment, preferably, after the raw materials are mixed in S3, the step of: removing impurities, washing, slicing and drying the raw materials accordingly is also included.

[0048] Example 2

[0049] The present invention provides a technical solution: application of royal jelly protein in Alzheimer's disease drugs, comprising the following steps:

[0050] S1. Extracting royal jelly protein: extracting royal jelly protein from royal jelly and setting aside;

[0051] S2. Preparation of drug raw materials: Prepare the following raw materials by weight: 30 parts of royal jelly protein, 15 parts of red peony root, 40 parts of astragalus, 40 parts of salvia miltiorrhiza, 40 parts of epimedium, 40 parts of polygala, 20 parts of chuanxiong, 20 parts of yizhiren, 20 parts of angelica, 20 parts of poria, 25 parts of polygonum multiflorum;

[0052] S3. Raw material mixing: mixing the above raw materials to obtain a mixed raw material drug;

[0053] S4. The mixed raw materials are decocted twice with water, the first time the amount of water added is 6 times the total weight of the mixed raw materials, and the decoction is performed for 80 minutes, and the second time the amount of water added is 4 times the total weight of the mixed raw materials, and the decoction is performed for 40 minutes. Filter after each decoction, combine the two filtrates, and concentrate under reduced pressure to obtain an extract, that is, a drug for treating Alzheimer's disease containing royal jelly protein.

[0054] In this embodiment, preferably, the specific steps of extracting royal jelly protein in S1 are:

[0055] S101. The buffer solution is prepared by potassium hydroxide, and then royal jelly is added into the buffer solution for mixing to obtain a mixed solution;

[0056] S102. The mixed solution obtained in S101 is filtered to remove large particles of impurities in the mixed solution to obtain a filtrate;

[0057] S103. The filtrate obtained in S102 is centrifuged, and during the centrifugation, the centrifuge is operated under a low temperature environment, and then the upper layer of the centrifuged solution is filtered;

[0058] S104. The centrifuged supernatant obtained in S103 is precipitated with royal jelly protein by PEG and antifreeze, and the solution is stirred at all times during the precipitation process by PEG and antifreeze;

[0059] S105. The royal jelly protein obtained in S104 is purified by ion exchange chromatography. The royal jelly protein is purified and concentrated by the mutual charge interaction between the royal jelly protein and the ion exchange resin to obtain the royal jelly protein.

[0060] In this embodiment, preferably, the buffer in S101 is Tris-HCl buffer, the concentration of the Tris-HCl buffer is 0.08 mol / L, and the pH is 7.9.

[0061] In this embodiment, preferably, after the raw materials are mixed in S3, the step of: removing impurities, washing, slicing and drying the raw materials accordingly is also included.

[0062] Example 3

[0063] The present invention provides a technical solution: application of royal jelly protein in Alzheimer's disease drugs, comprising the following steps:

[0064] S1. Extracting royal jelly protein: extracting royal jelly protein from royal jelly and setting aside;

[0065] S2. Preparation of drug raw materials: Prepare the following raw materials by weight: 20 parts of royal jelly protein, 10 parts of red peony root, 30 parts of astragalus, 30 parts of salvia miltiorrhiza, 30 parts of epimedium, 30 parts of polygala, 15 parts of chuanxiong, 15 parts of yizhiren, 15 parts of angelica, 15 parts of poria, 20 parts of polygonum multiflorum;

[0066] S3. Raw material mixing: mixing the above raw materials to obtain a mixed raw material drug;

[0067] S4. The mixed raw materials are decocted twice with water, the first time the amount of water added is 4 times the total weight of the mixed raw materials, and the decoction is performed for 70 minutes, and the second time the amount of water added is 3 times the total weight of the mixed raw materials, and the decoction is performed for 30 minutes. Filter after each decoction, combine the two filtrates, and concentrate under reduced pressure to obtain an extract, that is, a drug for treating Alzheimer's disease containing royal jelly protein.

[0068] In this embodiment, preferably, the specific steps of extracting royal jelly protein in S1 are:

[0069] S101. The buffer solution is prepared by potassium hydroxide, and then royal jelly is added into the buffer solution for mixing to obtain a mixed solution;

[0070] S102. The mixed solution obtained in S101 is filtered to remove large particles of impurities in the mixed solution to obtain a filtrate;

[0071] S103. The filtrate obtained in S102 is centrifuged, and during the centrifugation, the centrifuge is operated under a low temperature environment, and then the upper layer of the centrifuged solution is filtered;

[0072] S104. The centrifuged supernatant obtained in S103 is precipitated with royal jelly protein by PEG and antifreeze, and the solution is stirred at all times during the precipitation process by PEG and antifreeze;

[0073] S105. The royal jelly protein obtained in S104 is purified by ion exchange chromatography. The royal jelly protein is purified and concentrated by the mutual charge interaction between the royal jelly protein and the ion exchange resin to obtain the royal jelly protein.

[0074] In this embodiment, preferably, the buffer in S101 is Tris-HCl buffer, the concentration of the Tris-HCl buffer is 0.06 mol / L, and the pH is 7.5.

[0075] In this embodiment, preferably, after the raw materials are mixed in S3, the step of: removing impurities, washing, slicing and drying the raw materials accordingly is also included.

[0076] Example 4

[0077] See also Figure 1-Figure 4 , a preparation device for royal jelly protein in a drug for Alzheimer's disease, comprising a fixed base 1, three groups of legs 2 are welded on the upper surface of the fixed base 1, a mixing barrel 3 is welded on the top of the three groups of legs 2, four groups of support rods 4 are welded on the top of the mixing barrel 3, a cross plate 5 is welded on the top of the four groups of support rods 4, a first servo motor 6 is fixedly installed at the middle position of the upper surface of the cross plate 5, the output end of the first servo motor 6 is transmission-connected to a first rotating shaft 7, the bottom end of the first rotating shaft 7 is connected to a connecting rod 8 through a fixing component, and the other end of the connecting rod 8 is below the surface A stirring rod 9 is welded on the surface, a liquid outlet 10 is provided at the bottom of the mixing barrel 3, a liquid outlet valve 11 is installed on the liquid outlet 10, a cushion seat 12 is fixedly provided on the upper surface of the fixed base 1, a filter barrel 13 is rotatably connected to the upper surface of the cushion seat 12 through a driving component, a funnel-shaped filter screen 14 is provided on the top of the filter barrel 13 through a snap-on component, a discharge port 15 is provided on one side of the bottom of the filter barrel 13, a discharge valve 16 is fixedly installed on the discharge port 15, and a guide plate 17 is provided at the inner bottom of the filter barrel 13.

[0078] In this embodiment, preferably, in order to facilitate the disassembly and assembly of the connecting rod 8 and the first rotating shaft 7, the fixing assembly includes a protrusion 18 and a fastening bolt 19, and the protrusion 18 is provided in two groups. The two groups of protrusions 18 are welded to the outer surface of the connecting rod 8, and the two groups of protrusions 18 are fixed on both sides of the first rotating shaft 7 by the fastening bolts 19.

[0079] In this embodiment, preferably, the driving assembly includes a receiving groove 20, a second servo motor 21 and a second rotating shaft 22, the receiving groove 20 is arranged inside the cushion seat 12, the second servo motor 21 is installed in the receiving groove 20, the bottom end of the second rotating shaft 22 is transmission-connected to the output end of the second servo motor 21, and the top of the second rotating shaft 22 is fixedly connected to the bottom of the filter barrel 13; the second rotating shaft 22 is driven to rotate by the second servo motor 21, the second rotating shaft 22 drives the filter barrel 13 to rotate, and the filter barrel 13 drives the funnel-shaped filter screen 14 to rotate, and the eccentricity principle is used to make the outer side of the funnel-shaped filter screen 14 located at the liquid outlet 10 for material reception and filtration, thereby increasing the filtering effect and reducing the possibility of clogging.

[0080] In this embodiment, preferably, in order to facilitate the rotation of the filter barrel 13, a circle of balls 23 are annularly arranged on the upper surface of the seat 12, and the balls 23 are rotatably connected to the seat 12, and the bottom of the filter barrel 13 is arranged on the upper surface of the balls 23.

[0081] In this embodiment, preferably, in order to facilitate the disassembly and assembly of the funnel-shaped filter screen 14, the snap-on assembly includes a fixing ring 24 and a limiting ring 25, the fixing ring 24 is fixedly arranged on the upper surface of the funnel-shaped filter screen 14, the limiting ring 25 is fixedly arranged on the lower surface of the fixing ring 24, and the limiting ring 25 is arranged on the outer surface of the filter barrel 13.

[0082] Principles and advantages of the present invention:

[0083] The present invention applies royal jelly protein to the drug for treating Alzheimer's disease, and utilizes that royal jelly protein contains rich vitamin B and a large amount of protein, has strong bactericidal ability, has the effect of inhibiting the growth of cancer cells, and can improve nutrition, supplement brain power and promote human metabolism, thereby improving the effect of treating Alzheimer's disease and reducing the probability of Alzheimer's disease; the first servo motor 6 drives the first rotating shaft 7 to rotate, and the rotation of the first rotating shaft 7 drives the stirring rod 9 to rotate, so that the stirring rod 9 mixes the materials inside the mixing barrel 3, thereby facilitating the mixing of materials, and at the same time, using the fixed component, it is convenient to disassemble and clean the stirring rod 9; by setting the filter barrel 13, the funnel-shaped filter screen 14 is used to filter the mixed liquid discharged from the liquid outlet 10, so as to facilitate the removal of large particle impurities in the mixed liquid, and at the same time, using the eccentricity principle, the outer side of the funnel-shaped filter screen 14 is located at the liquid outlet 10 for receiving and filtering, so that the large particle impurities fall to the center of the funnel-shaped filter screen 14, thereby increasing the filtering effect and reducing the possibility of clogging.

[0084] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. The application of royal jelly protein in drugs for Alzheimer's disease is characterized by: The following steps are involved: S1. Extracting royal jelly protein: extracting royal jelly protein from royal jelly and setting aside; S2. Preparation of drug raw materials: Prepare the following raw materials by weight: 10-30 parts of royal jelly protein, 5-15 parts of red peony root, 20-40 parts of astragalus, 20-40 parts of salvia miltiorrhiza, 20-40 parts of epimedium, 20-40 parts of polygala, 10-20 parts of chuanxiong, 10-20 parts of yizhiren, 10-20 parts of angelica, 10-20 parts of poria, 15-25 parts of polygonum multiflorum; S3. Raw material mixing: mixing the above raw materials to obtain a mixed raw material drug; S4. The mixed raw materials are decocted twice with water, the first time the amount of water added is 2-6 times the total weight of the mixed raw materials, and the decoction is performed for 60-80 minutes, and the second time the amount of water added is 1-4 times the total weight of the mixed raw materials, and the decoction is performed for 20-40 minutes. Filter after each decoction, combine the two filtrates, and concentrate under reduced pressure to obtain an extract, that is, a drug for treating Alzheimer's disease containing royal jelly protein.

2. The use of royal jelly protein in Alzheimer's disease drugs according to claim 1, characterized in that: Preparation of the medicinal raw materials in S2: prepare the raw materials according to the following weight portions: 20 parts of royal jelly protein, 10 parts of red peony root, 30 parts of astragalus, 30 parts of salvia miltiorrhiza, 30 parts of epimedium, 30 parts of polygala, 15 parts of chuanxiong, 15 parts of alpinia oxyphylla, 15 parts of angelica, 15 parts of poria, and 20 parts of polygonum multiflorum.

3. The use of royal jelly protein in Alzheimer's disease drugs according to claim 1, characterized in that: The specific steps of extracting royal jelly protein in S1 are: S101. The buffer solution is prepared by potassium hydroxide, and then royal jelly is added into the buffer solution for mixing to obtain a mixed solution; S102. The mixed solution obtained in S101 is filtered to remove large particles of impurities in the mixed solution to obtain a filtrate; S103. The filtrate obtained in S102 is centrifuged, and during the centrifugation, the centrifuge is operated under a low temperature environment, and then the upper layer of the centrifuged solution is filtered; S104. The centrifuged supernatant obtained in S103 is precipitated with royal jelly protein by PEG and antifreeze, and the solution is stirred at all times during the precipitation process by PEG and antifreeze; S105. The royal jelly protein obtained in S104 is purified by ion exchange chromatography. The royal jelly protein is purified and concentrated by the mutual charge interaction between the royal jelly protein and the ion exchange resin to obtain the royal jelly protein.

4. The use of royal jelly protein in Alzheimer's disease drugs according to claim 3, characterized in that: The buffer used in S101 is Tris-HCl buffer, the concentration of the Tris-HCl buffer is 0.02-0.08 mol / L, and the pH is 7.3-7.

9.

5. The use of royal jelly protein in Alzheimer's disease drugs according to claim 1, characterized in that: After the raw materials are mixed in S3, the following steps are further performed: removing impurities, washing, slicing and drying the raw materials.

6. Equipment for preparing royal jelly protein in drugs for Alzheimer's disease, characterized by: The invention comprises a fixed base (1), wherein three groups of legs (2) are welded to the upper surface of the fixed base (1), a mixing barrel (3) is welded to the top of the three groups of legs (2), four groups of support rods (4) are welded to the top of the mixing barrel (3), a cross plate (5) is welded to the top of the four groups of support rods (4), a first servo motor (6) is fixedly installed at the middle position of the upper surface of the cross plate (5), the output end of the first servo motor (6) is connected to the first rotating shaft (7) in a transmission manner, the bottom end of the first rotating shaft (7) is connected to a connecting rod (8) through a fixing assembly, and a stirring rod (9) is welded to the lower surface of the other end of the connecting rod (8), The bottom of the mixing barrel (3) is connected to a liquid outlet (10), and a liquid outlet valve (11) is installed on the liquid outlet (10). A cushion seat (12) is fixedly installed on the upper surface of the fixed base (1), and the upper surface of the cushion seat (12) is rotatably connected to a filter barrel (13) through a driving component. A funnel-shaped filter screen (14) is provided on the top of the filter barrel (13) through a snap-on component. A discharge port (15) is connected to one side of the bottom of the filter barrel (13), and a discharge valve (16) is fixedly installed on the discharge port (15). A guide plate (17) is provided at the inner bottom of the filter barrel (13).

7. The preparation device of royal jelly protein in Alzheimer's disease medicine according to claim 6, characterized in that: The fixing assembly comprises a protrusion (18) and a fastening bolt (19), wherein the protrusion (18) is provided in two groups, the two groups of protrusions (18) are welded to the outer surface of the connecting rod (8), and the two groups of protrusions (18) are fixedly arranged on both sides of the first rotating shaft (7) by the fastening bolts (19).

8. The preparation device of royal jelly protein in Alzheimer's disease medicine according to claim 6, characterized in that: The driving assembly comprises a receiving groove (20), a second servo motor (21) and a second rotating shaft (22); the receiving groove (20) is arranged inside the cushion seat (12); the second servo motor (21) is installed in the receiving groove (20); the bottom end of the second rotating shaft (22) is transmission-connected to the output end of the second servo motor (21); and the top of the second rotating shaft (22) is fixedly connected to the bottom of the filter barrel (13).

9. The preparation device of royal jelly protein in Alzheimer's disease medicine according to claim 7, characterized in that: A circle of balls (23) is arranged in an annular shape on the upper surface of the cushion seat (12), the balls (23) are rotatably connected to the cushion seat (12), and the bottom of the filter barrel (13) is arranged on the upper surface of the balls (23).

10. The preparation device of royal jelly protein in Alzheimer's disease medicine according to claim 6, characterized in that: The clamping assembly comprises a fixing ring (24) and a limiting ring (25), wherein the fixing ring (24) is fixedly arranged on the upper surface of the funnel-shaped filter screen (14), the limiting ring (25) is fixedly arranged on the lower surface of the fixing ring (24), and the limiting ring (25) is arranged on the outer surface of the filter barrel (13).

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