Method for preparing liquid fertilizer from seriola quinqueradiata leftovers

Abstract

The invention relates to the technical field of liquid fertilizers, in particular to a method for preparing a liquid fertilizer from seriola quinqueradiata leftovers. The method comprises the following steps: by taking seriola quinqueradiata leftovers as a raw material, treating to obtain minced fillet slurry, adding protease into the minced fillet slurry for enzymolysis, and separating and collecting peptone-containing liquid after enzymolysis, thereby obtaining the liquid fertilizer. The obtained liquid fertilizer contains high-concentration amino acid and trace elements and macroelements in a proper proportion, can provide high-quality nutrients for crops, and promotes germination and growth of the crops such as wheat. According to the method, a sustainable and resource-efficient way is provided for preparation of the liquid fertilizer, by means of reutilization of the seriola quinqueradiata leftovers, the requirement for traditional chemical raw materials is reduced, the environmental burden is reduced, and powerful support is provided for development of sustainable agriculture.

Description

Technical Field

[0001] The invention relates to the technical field of liquid fertilizers, and in particular to a method for preparing liquid fertilizer by utilizing yellowtail amberjack waste. Background Art

[0002] With the rapid development of my country's rural economy and the increasing attention to the green environment, the research and development and utilization of new and efficient fertilizers have become a top priority. The preparation of traditional liquid fertilizers usually requires the use of a large amount of chemical raw materials, such as ammonium nitrate and diammonium phosphate. Although these fertilizers can provide the nutrients required for plant growth, they have certain negative effects on the environment. The long-term and unreasonable use of chemical fertilizers will lead to a variety of adverse factors that have a negative impact on the soil and the environment, including soil acidification, compaction, and decreased fertility.

[0003] As a natural product, fish protein liquid fertilizer has many advantages, including being non-toxic and harmless to humans and animals, non-polluting to the environment, convenient to transport, and easy to apply. Its active ingredients can be fully absorbed and utilized by crops, will not remain in the soil, will not cause any harm to crops and soil, and has a significant role in promoting the growth and development of plants. Therefore, it is regarded as the preferred fertilizer for green food production. At the same time, the rapid development of my country's aquaculture and aquatic product processing industry can further promote its development. Summary of the invention

[0004] The invention aims to provide a method for preparing liquid fertilizer by utilizing yellow amberjack waste.

[0005] To achieve the above purpose, the present invention adopts the following technical solution:

[0006] The invention discloses a method for preparing liquid fertilizer by using yellowtail fish waste. The yellowtail fish waste is used as raw material, and fish paste is obtained after processing. Protease is added into the fish paste for enzymolysis. After the enzymolysis, liquid containing peptone is separated and collected to obtain liquid fertilizer.

[0007] The yellowtail fish scraps are ground to obtain fish paste, which is mixed with flavor protease at 50-55°C for enzymolysis for 5-6 hours, and then centrifuged to collect liquid containing peptone, which is liquid fertilizer.

[0008] The added amount of the enzyme accounts for 0.8-1.2% of the weight ratio of the fish paste, and the material-liquid ratio (the ratio of the fish paste to water in the system) is 1:1-1:5 g / mL.

[0009] The yellowtail scraps are sourced from one or more of fish scales, fish viscera, fish heads, fish bones, feed residues and discarded fish pieces in fish processing, aquatic product processing or aquaculture.

[0010] An application of liquid fertilizer prepared by using yellowtail fish waste, and application of the liquid fertilizer in promoting the growth and development of plants.

[0011] Compared with the prior art, the present invention has the following advantages:

[0012] The present invention provides a more environmentally friendly, efficient and multifunctional method for preparing liquid fertilizer. The implementation of the method of the present invention can make full use of waste resources, convert aquatic waste into valuable agricultural resources, and reduce the demand for chemical raw materials, thereby reducing pollution to the environment. The liquid fertilizer of the present invention has a simple preparation process, is rich in amino acids and trace elements, helps to improve soil quality, increases plant growth rate, is suitable for different crops and soil types, and is expected to bring important benefits to modern agriculture and environmental protection. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 A comparison chart of major elements in fish protein liquid fertilizer is provided for an embodiment of the present invention.

[0014] Figure 2 A comparison chart of trace elements in fish protein liquid fertilizer is provided for an embodiment of the present invention.

[0015] Figure 3 The present invention provides an effect diagram of the effect of fish protein liquid fertilizer on wheat germination. Specific implementation method:

[0016] The present invention will be further described below in conjunction with specific examples, and the advantages and features of the present invention will become clearer as the description proceeds. However, the embodiments are exemplary only and do not constitute any limitation to the scope of the present invention. It should be understood by those skilled in the art that the details and forms of the technical solution of the present invention may be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the scope of protection of the present invention.

[0017] The present invention removes impurities and cleans the yellowtail fish waste, and then processes the waste into uniform fish paste by using a process such as mincing; the fish paste is combined with a protease to perform an enzymatic hydrolysis reaction; and a clear liquid portion, i.e., liquid fertilizer, is separated from the enzymatically hydrolyzed mixture by filtering and centrifuging. The obtained liquid fertilizer contains a high concentration of amino acids and trace elements and macroelements in an appropriate proportion, provides high-quality nutrients for crops, and can promote the germination and growth of crops such as wheat. The method converts waste aquatic resources into organic fertilizers, which not only realizes efficient resource utilization, but also provides strong support for the realization of sustainable agriculture and green food production.

[0018] The structures, proportions, sizes, etc. drawn in the drawings of the present invention are only used to match the contents disclosed in the specification so as to facilitate understanding and reading by persons familiar with the technology. They are not used to limit the conditions under which the present invention can be implemented, and therefore have no substantial technical significance. Any modification of the structure, change in the proportion relationship, or adjustment of the size should still fall within the scope of the technical contents disclosed in the present invention without affecting the effects and purposes that can be achieved by the present invention.

[0019] The experimental methods in the following examples without specific experimental conditions are usually carried out according to conventional experimental conditions or the experimental conditions recommended by the manufacturer. The materials and reagents used are commercially available unless otherwise specified.

[0020] Unless otherwise specified, the devices used in the present invention are all conventional devices in the art.

[0021] The present invention will be further described below in conjunction with embodiments:

[0022] Embodiment 1:

[0023] A yellowtail fish waste liquid fertilizer, the preparation method of which comprises the following steps:

[0024] 1) Collect fresh yellowtail fish scraps, remove impurities and wash them; cut the washed scraps into blocks of appropriate size; put the blocks of scraps into a tissue grinder and grind them into a mud, that is, fish paste.

[0025] 2) Take 50g of fish paste and place it in a 500mL beaker, add 100mL of water and stir evenly, that is, the material-liquid ratio is 1:2. The enzyme used for enzymatic hydrolysis is flavor protease, and the amount of enzyme added is 1%, that is, 0.5g. The enzyme is added to the beaker, the enzymatic hydrolysis reaction temperature is 53℃, and the enzymatic hydrolysis time is 5h. The reaction ends at 90℃ for 30min of inactivation.

[0026] 3) The mixture after enzymatic hydrolysis is filtered and centrifuged, placed in a 50 mL centrifuge tube, and centrifuged at 4° C., 6500 r / min for 15 min to remove solid waste, and the remaining liquid portion is the obtained liquid fertilizer.

[0027] The obtained liquid fertilizer is rich in amino acids, and its pH value is 5.25. The major elements of liquid fertilizer were analyzed with reference to the "Determination of Total Nitrogen, Phosphorus and Potassium Contents of Water-soluble Fertilizers", and the contents of nitrogen, phosphorus and potassium were 0.86%, 0.13% and 0.34% respectively. The trace elements were determined by inductively coupled plasma mass spectrometry, and the contents of boron, manganese, iron, copper and zinc were 7.9ng / mL, 0.9ng / mL, 2.1ng / mL, 3.2ng / mL and 0.5ng / mL respectively.

[0028] Embodiment 2:

[0029] A yellowtail fish waste liquid fertilizer, the preparation method of which comprises the following steps:

[0030] 1) Collect fresh yellowtail fish scraps, remove impurities and wash them; cut the washed scraps into blocks of appropriate size; put the blocks of scraps into a tissue grinder and grind them into a mud, that is, fish paste.

[0031] 2) Take 50g of fish paste and place it in a 500mL beaker, add 250mL of water and stir evenly, that is, the material-liquid ratio is 1:5. The enzyme used for enzymatic hydrolysis is flavor protease, and the amount of enzyme added is 1%, that is, 0.5g. The enzyme is added to the beaker, the enzymatic hydrolysis reaction temperature is 53℃, and the enzymatic hydrolysis time is 5h. The reaction ends and inactivates at 90℃ for 30min.

[0032] 3) The mixture after enzymatic hydrolysis is filtered and centrifuged, placed in a 50 mL centrifuge tube, and centrifuged at 4° C., 6500 r / min for 15 min to remove solid waste, and the remaining liquid portion is the obtained liquid fertilizer.

[0033] The obtained liquid fertilizer is rich in amino acids, and its pH value is 5.31. The major elements of liquid fertilizer were analyzed with reference to the "Determination of Total Nitrogen, Phosphorus and Potassium Contents of Water-soluble Fertilizers", and the contents of nitrogen, phosphorus and potassium were 0.51%, 0.06% and 0.03% respectively. The trace elements were determined by inductively coupled plasma mass spectrometry, and the contents of boron, manganese, iron, copper and zinc were 3ng / mL, 2.2ng / mL, 1.7ng / mL, 1.0ng / mL and 1.0ng / mL respectively.

[0034] The comparison of the major elements and trace elements in the liquid fertilizer obtained in Example 1 and Example 2 is as follows Figure 1 and Figure 2 Specifically, the content of macroelements in the liquid fertilizer obtained when the liquid-to-solid ratio is 1:2 is higher, while the content of trace elements is different. These differences are related to the characteristics of the enzymatic hydrolysis process, rather than the ordinary liquid concentration process. Adjusting different enzymatic hydrolysis conditions under certain conditions has a direct impact on the content and ratio of elements.

[0035] Example 3: Wheat germination experiment

[0036] Specifically:

[0037] The fish protein liquid fertilizer obtained in Example 1 of the present invention was diluted 300 times and 500 times respectively for subsequent use. To ensure the accuracy of the experiment, wheat seeds with full grains, large individuals, strong vitality and uniform size were selected, and the seeds were sterilized with 75% alcohol for 1 min, and rinsed with sterile water 5 times. The wheat seeds were then blanched and the wheat was placed in 55°C warm water for 10 min. After the blanching treatment, the experimental seeds were placed in the diluted fish protein liquid fertilizer and clean water respectively, and the soaking time was 8 hours. Ensure that the liquid used in the soaking process is sterilized. The treated seeds were evenly placed in a glass culture dish with a diameter of 15 cm containing two filter papers. Three parallel experiments were set for each group, and the seeds were soaked in sterile water as the control group. In order to keep the seeds moist, the filter paper fully absorbed the sterilized water. The germination of wheat seeds was recorded for 7 consecutive days, including the number of germinations and the germination speed, and the germination rate, germination potential and germination index were calculated. Figure 3 The effect of liquid fertilizer on wheat germination was obtained by using the above-mentioned Example 1.

[0038] The calculation formula is as follows:

[0039] Germination rate (GR) = (number of germinated seeds on the 7th day / number of test seeds) × 100%

[0040] Germination potential (GP) = number of seeds germinated at the germination peak (day 3) / number of seeds tested × 100%

[0041] Germination index (PI) = ∑ (Gt / Dt)

[0042] Gt is the number of germinations per day during the final period of the germination test, Dt is the number of germination days, and ∑ is the total

[0043] The results show that the use of diluted fish protein liquid fertilizer for soaking wheat seeds in this embodiment can effectively promote the germination of wheat seeds compared with soaking seeds only with sterile water. Specifically, compared with the control group, the wheat seeds using fish protein liquid fertilizer showed a higher germination rate, germination potential and germination index. When the liquid fertilizer is diluted 500 times, it is better to promote the germination of wheat seeds.

[0044] This result emphasizes the role of fish protein liquid fertilizer in promoting the growth and development of wheat seeds. Its rich amino acid and protein content may provide the seeds with necessary nutrition and growth stimulation. Since germination rate, germination potential and germination index are key parameters for evaluating seed growth and health, the results of this experiment show that fish protein liquid fertilizer can be a powerful tool to improve wheat yield and quality, and it is also expected to reduce the demand for chemical fertilizers in agricultural production, thereby promoting the development of sustainable agriculture.

[0045] The above embodiments further illustrate the purpose and technical solutions of the present invention. It should be noted that the above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

[0046] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present disclosure will not further describe various possible combinations.

[0047] In addition, the various embodiments of the present disclosure may be arbitrarily combined, and as long as they do not violate the concept of the present disclosure, they should also be regarded as the content invented by the present disclosure.

Claims

1. A method for preparing liquid fertilizer using yellowtail fish scraps, characterized in that: The yellowtail fish waste is used as raw material, and fish paste is obtained after processing. Protease is added into the fish paste for enzymolysis, and the peptone-containing liquid is separated and collected after enzymolysis to obtain liquid fertilizer.

2. The method for preparing liquid fertilizer using yellowtail fish scraps according to claim 1, wherein: The yellowtail fish scraps are ground to obtain fish paste, which is mixed with flavor protease at 50-55°C for enzymolysis for 5-6 hours, and then centrifuged to collect liquid containing peptone, which is liquid fertilizer.

3. The method for preparing liquid fertilizer using yellowtail offal according to claim 1 or 2, characterized in that: The added amount of the enzyme accounts for 0.8-1.2% of the weight ratio of the fish paste, and the material-liquid ratio is 1:1-1:5 g / mL.

4. The method for preparing liquid fertilizer using yellowtail fish scraps according to claim 1 or 2, characterized in that: The yellowtail scraps are sourced from one or more of fish scales, fish viscera, fish heads, fish bones, feed residues and discarded fish pieces in fish processing, aquatic product processing or aquaculture.

5. An application of yellowtail fish scraps for preparing liquid fertilizer according to claim 1, characterized in that: The liquid fertilizer is used to promote the growth and development of plants.

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