Method for repairing bone keratin cultural relics by inducing hydroxyapatite through microorganisms

Abstract

The invention discloses a method for repairing bone keratin cultural relics by inducing hydroxyapatite through microorganisms, and belongs to the technical field of cultural relic repairing. The method comprises the following steps: dissolving animal bone meal in an acid solution, and then adjusting the pH value to obtain a bone meal flocculation solution; centrifuging the bone meal flocculation solution, and removing supernate to obtain flocculate; the flocculate is applied to a to-be-repaired area, the urease-producing microbial liquid and the urea solution are applied to the to-be-repaired position of the bone keratin cultural relic in an equal-volume mode till glue joint is formed, finally, the to-be-repaired position is smeared with a protective material, and repairing is completed. According to the method, the pH is slowly increased through urease-producing microorganisms, and under the action of calcium salt and phosphate radicals, hydroxyapatite is slowly generated to complete repair of the bone keratin cultural relics; the repairing material has high consistency with the components of the bone keratin cultural relics, is non-toxic and harmless, and can fully restore the original appearance of the bone keratin cultural relics; and short-time high efficiency is achieved, fracture and damage of bone keratin cultural relics can be repaired in time, and the repaired material is high in strength.

Description

Technical Field

[0001] The invention relates to the technical field of cultural relics restoration, and in particular to a method for repairing bone keratin cultural relics by using microorganisms to induce hydroxyapatite. Background Art

[0002] Bone and horn artifacts are an important part of human historical and cultural heritage. They record the development of human society and cultural evolution, have extremely high historical value, artistic value and scientific value, and reveal the origin of life and the evolution of species. However, due to the organic properties of bone and horn artifacts, they are extremely sensitive to environmental changes and are susceptible to corrosion, degradation and biological invasion. They are gradually facing a series of problems such as cracks and even fractures, which seriously threaten their preservation and inheritance. The formation of cracks in bone and horn artifacts not only affects the beauty and integrity of the artifacts themselves, but once cracks occur, they will further threaten the integrity of the artifacts and accelerate the weathering and destruction process of the artifacts.

[0003] However, existing methods for repairing and protecting damaged bone and keratin cultural relics include calcium phosphate bonding (patent 202311343720.3), gypsum bonding (patent 201911073513.4), inorganic fiber material reinforcement (patent 202110415412.1), organic material protective agents (patent 202410635630.X) and other means. These methods have certain effects, but they also have many limitations and disadvantages. For example, most of the above-mentioned repair materials are chemical materials and have poor compatibility with the main materials. Secondly, there are problems such as irreversibility, material aging, aesthetic conflicts, poor environmental adaptability and high maintenance costs.

[0004] In order to solve the problem of fracture and damage of bone and keratin cultural relics, it is urgent to develop a highly efficient and environmentally friendly technology to repair cracks in bone and keratin cultural relics in order to meet the requirements of being non-toxic, harmless and not introducing impurities. The repair material should have good compatibility with stone cultural relics, low color difference and weather resistance. Summary of the invention

[0005] In order to solve the above technical problems, the purpose of the present invention is to provide a method for repairing bone keratin cultural relics with microorganism-induced hydroxyapatite, so as to solve the problems of poor compatibility of existing bone keratin cultural relics repair materials with the main materials, irreversibility, easy aging of materials, unsightly appearance, poor environmental adaptability and high maintenance costs.

[0006] The technical solution of the present invention to solve the above technical problems is as follows:

[0007] A method for repairing bone keratin artifacts by inducing hydroxyapatite with microorganisms comprises the following steps:

[0008] (1) dissolving animal bone powder in an acid solution, and then adjusting the pH to obtain a bone powder flocculation solution;

[0009] (2) centrifuging the bone powder flocculation solution and removing the supernatant to obtain floccules;

[0010] (3) applying the flocculant obtained in step (2) to the area to be repaired, and applying the urease-producing microbial solution and the urea solution in the same proportion to the position of the bone and keratin cultural relic to be repaired until a bond is formed, and finally applying a protective material to the position to be repaired to complete the repair.

[0011] The method of the present invention adopts a repair material that is highly consistent with bone and keratin cultural relics, and can repair the damaged parts of bone and keratin cultural relics in an efficient and environmentally friendly manner. It also has the advantages of strong compatibility, low cost, short-time high efficiency, small color difference, and non-toxicity.

[0012] Furthermore, the animal bone meal in step (1) includes at least one of mammal bone meal, fish bone meal, reptile bone meal and bird bone meal.

[0013] Furthermore, the particle size of the animal bone meal is less than 500 μm and the water content is less than 20%.

[0014] Furthermore, before restoration, the mineral composition of the bone and keratin cultural relics to be restored is tested, and animal bone powder with similar calcium and phosphorus contents is selected according to the ratio of calcium and phosphorus contents in the bone and keratin cultural relics to be restored.

[0015] Furthermore, in step (1), the acid solution is a hydrochloric acid solution with a concentration of 1-2 mol / L; and the mass ratio of the animal bone powder to the acid solution is 0.5-2:1-3.

[0016] Preferably, the acid solution in step (1) is a hydrochloric acid solution with a concentration of 1 mol / L; and the mass ratio of animal bone powder to the acid solution is 1:2.

[0017] Furthermore, in step (1), dilute ammonia water is used to adjust the pH to 7-9.

[0018] Preferably, in step (1), dilute ammonia water is used to adjust the pH to 8.

[0019] Furthermore, the urease activity of the urease-producing microbial culture liquid in step (3) is 10-30 U / mL.

[0020] Preferably, the urease activity of the urease-producing microbial culture liquid in step (3) is 15 U / mL.

[0021] Furthermore, the concentration of the urea solution in step (3) is 1-3 mol / L.

[0022] Preferably, the concentration of the urea solution in step (2) is 1 mol / L.

[0023] Furthermore, in step (2), the protective material comprises any one of polyurethane, cotton and sponge.

[0024] The present invention has the following beneficial effects:

[0025] The present invention provides a method for repairing bone keratin cultural relics by microbial induction of hydroxyapatite, wherein the pH is slowly increased by urease-producing microorganisms, and hydroxyapatite is slowly generated in the presence of calcium salts and phosphates to complete the repair of bone keratin cultural relics. The repair material used in the present invention has a high degree of consistency with the hydroxyapatite of the bone keratin cultural relics component, is non-toxic and harmless, can fully restore the original appearance of the bone keratin cultural relics, and is highly compatible with the bone keratin cultural relics to be repaired. The method of the present invention has short-term high efficiency, can timely repair the fracture and damage of the bone keratin cultural relics, and the strength of the repaired material is high. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 The XRD pattern of mammal bone powder used in Example 1;

[0027] Figure 2 The XRD pattern of the cement formed in step (4) of Example 1;

[0028] Figure 3 This is the XRD spectrum of the flocs formed in step (4) of Comparative Example 1. DETAILED DESCRIPTION

[0029] The principles and features of the present invention are described below in conjunction with the accompanying drawings. The examples are only used to explain the present invention and are not used to limit the scope of the present invention. If specific conditions are not specified in the embodiments, they are carried out according to normal conditions or conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0030] Embodiment 1:

[0031] A method for repairing bone keratin artifacts by inducing hydroxyapatite with microorganisms comprises the following steps:

[0032] (1) The composition of the bone horn cultural relics to be repaired was detected by in-situ XRD method, and it was found that its main component was hydroxyapatite. According to the ratio of calcium and phosphorus content in the bone horn cultural relics to be repaired, mammal bone powder (deer antler bone powder) with similar calcium and phosphorus content was selected. Its composition is as follows Figure 1 shown.

[0033] (2) Deer antler bone powder (particle size 300 μm, water content 10%) was dissolved in 1 mol / L hydrochloric acid to obtain a mammalian bone powder solution with a pH of 2, and then the pH was slowly adjusted to 8 with dilute ammonia water, and the solution was centrifuged to obtain flocs.

[0034] (3) Prepare a Sarcina pasteurii bacterial solution with a urease activity of 15 U / mL and a 1 mol / L urea solution.

[0035] (4) The mammalian bone powder solution flocculant obtained in step (2), the microbial solution obtained in step (3), and the urea solution are slowly applied to the repaired position of the bone keratin cultural relic to be repaired until a cement is formed, wherein the main component of the cement is hydroxyapatite, such as Figure 2 shown.

[0036] (5) Apply protective sponge material to the area to be repaired for sealing and protection, thus completing the restoration of bone and keratin cultural relics.

[0037] After restoration in this embodiment, no impurities are introduced, the original appearance of the bone and keratin cultural relics is fully restored, and the strength of the material after restoration is high, meeting the requirements.

[0038] Embodiment 2:

[0039] A method for repairing bone keratin artifacts by inducing hydroxyapatite with microorganisms comprises the following steps:

[0040] (1) The composition of the bone horn cultural relics to be repaired was detected by in situ XRD method, and it was found that its main component was hydroxyapatite. According to the ratio of calcium and phosphorus content in the bone horn cultural relics to be repaired, mammal bone powder (deer antler bone powder) with similar calcium and phosphorus content was selected.

[0041] (2) Deer antler bone powder (particle size 300 μm, water content 10%) was dissolved in 1.5 mol / L hydrochloric acid to obtain a mammalian bone powder solution with a pH of 2, and then the pH was slowly adjusted to 8 with dilute ammonia water, and the solution was centrifuged to obtain flocs.

[0042] (3) Prepare a microbial culture solution with a urease activity of 20 U / mL and a 2 mol / L urea solution.

[0043] (4) The mammalian bone powder solution flocculant obtained in step (2), the microbial solution obtained in step (3), and the urea solution are slowly applied to the repaired position of the bone keratin cultural relic to be repaired until a cement is formed, wherein the main component of the cement is hydroxyapatite.

[0044] (5) Apply protective polyurethane material to the area to be repaired for sealing and protection, thus completing the restoration of the bone and keratin cultural relics.

[0045] Embodiment 3:

[0046] A method for repairing bone keratin artifacts by inducing hydroxyapatite with microorganisms comprises the following steps:

[0047] (1) The composition of the bone horn cultural relics to be repaired was detected by in situ XRD method, and it was found that its main component was hydroxyapatite. According to the ratio of calcium and phosphorus content in the bone horn cultural relics to be repaired, mammal bone powder (deer antler bone powder) with similar calcium and phosphorus content was selected.

[0048] (2) Deer antler bone powder (particle size 300 μm, water content 10%) was dissolved in 2 mol / L hydrochloric acid to obtain a mammalian bone powder solution with a pH of 2, and then the pH was slowly adjusted to 9 with dilute ammonia water, and the solution was centrifuged to obtain flocs.

[0049] (3) Prepare a microbial culture solution with a urease activity of 30 U / mL and a 3 mol / L urea solution.

[0050] (4) The mammalian bone powder solution flocculant obtained in step (2), the microbial solution obtained in step (3), and the urea solution are slowly applied to the repaired position of the bone keratin cultural relic to be repaired until a cement is formed, wherein the main component of the cement is hydroxyapatite.

[0051] (5) Apply protective cotton to the area to be repaired to seal and protect it, thus completing the restoration of the bone and keratin cultural relics.

[0052] Comparative Example 1:

[0053] A method for repairing bone and horny cultural relics, comprising the following steps:

[0054] (1) The composition of the bone horn cultural relics to be repaired was detected by in situ XRD method, and it was found that its main component was hydroxyapatite. According to the ratio of calcium and phosphorus content in the bone horn cultural relics to be repaired, mammal bone powder (deer antler bone powder) with similar calcium and phosphorus content was selected.

[0055] (2) Deer antler bone powder (particle size 300 μm, water content 10%) was dissolved in 1 mol / L hydrochloric acid to obtain a mammalian bone powder solution with a pH of 2, and then the pH was slowly adjusted to 8 with dilute ammonia water, and the solution was centrifuged to obtain flocs.

[0056] (3) Prepare 1 mol / L urea solution.

[0057] (4) The mammalian bone powder solution flocs obtained in step (2) and the urea solution obtained in step (3) are simultaneously and slowly applied to the position of the bone keratin cultural relic to be repaired, and the flocs are subjected to XRD analysis. The experimental results are as follows: Figure 3 As shown, its composition structure is quite different from that of hydroxyapatite, and it cannot fully restore the original appearance of bone and keratin artifacts. The strength of the repaired material is poor and its compatibility with the bone artifacts to be repaired is poor.

[0058] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for repairing bone keratin artifacts by inducing hydroxyapatite with microorganisms, characterized in that: The following steps are involved: (1) dissolving animal bone powder in an acid solution, and then adjusting the pH to obtain a bone powder flocculation solution; (2) centrifuging the bone powder flocculation solution and removing the supernatant to obtain floccules; (3) Applying the flocculant obtained in step (2) to the area to be repaired, and applying equal volumes of urease-producing microbial liquid and urea solution to the position of the bone and keratin cultural relic to be repaired until a bond is formed, and finally applying a protective material to the position to be repaired to complete the repair.

2. The method for repairing bone keratin artifacts by microbial induction of hydroxyapatite according to claim 1, characterized in that: The animal bone meal in step (1) includes at least one of mammal bone meal, fish bone meal, reptile bone meal and bird bone meal.

3. The method for repairing bone keratin artifacts by microbial induction of hydroxyapatite according to claim 2, characterized in that: The particle size of the animal bone powder is less than 500 μm and the water content is less than 20%.

4. The method for repairing bone keratin artifacts by microbial induction of hydroxyapatite according to claim 1, characterized in that: In the step (1), the acid solution is a hydrochloric acid solution with a concentration of 1-2 mol / L; the mass ratio of the animal bone powder to the acid solution is 0.5-2:1-3.

5. The method for repairing bone keratin artifacts by microbial induction of hydroxyapatite according to claim 1, characterized in that: In the step (1), dilute ammonia water is used to adjust the pH to 7-9.

6. The method for repairing bone keratin artifacts by microbial induction of hydroxyapatite according to claim 1, characterized in that: The urease activity of the urease-producing microbial culture liquid in the step (3) is 10-30 U / mL.

7. The method for repairing bone keratin artifacts by microbial induction of hydroxyapatite according to claim 1, characterized in that: The concentration of the urea solution in step (3) is 1-3 mol / L.

8. The method for repairing bone keratin artifacts by inducing hydroxyapatite with microorganisms according to claim 1, characterized in that: The protective material in step (3) includes any one of polyurethane, cotton and sponge.

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