Kit for gene detection of intestinal bacteria and use method

Abstract

The invention discloses a kit for gene detection of intestinal bacteria and a use method, and relates to the technical field of gene detection.The kit comprises a detection box, a storage plate and a test tube rack are arranged in the detection box, the test tube rack is circular, detection test tubes are annularly distributed on the test tube rack in an inserted mode, and the test tube rack is connected with a space installation mechanism and a positioning mechanism; a reagent filling mechanism and a centrifugal posture control mechanism are arranged on the storage plate, the space installation mechanism comprises a first positioning ball fixedly connected with the bottom support, the center of the test tube rack is installed in cooperation with the outer ring of the first positioning ball, a plurality of arc-shaped racks are annularly distributed on the edge, matched with the test tube rack, of the storage plate, and arc-shaped sliding grooves are formed in the arc-shaped racks; the test tube rack and the arc-shaped sliding groove are installed in a sliding mode, the test tube rack is further connected with a rotation driving assembly, multi-angle centrifugal control over the detection test tubes is achieved through the rotation driving assembly and the centrifugal posture control mechanism, and the mixing effect of florae and reagents in the detection test tubes is effectively improved.

Description

Technical Field

[0001] The present invention relates to the technical field of gene detection, and in particular to a kit for intestinal bacteria gene detection and a use method thereof. Background Art

[0002] Intestinal flora genetic testing is a genetic testing method designed to detect the genetic information of microorganisms in the human intestine. Intestinal flora genetic testing can determine the composition and function of the intestinal flora by analyzing and detecting the DNA and RNA of intestinal microorganisms. It can reflect information such as the types, species dominance, proportions and niche accumulation of microorganisms in the intestine. In addition, intestinal flora genetic testing can also be used to evaluate the stability of the intestinal flora, as well as the relationship between the intestinal flora and intestinal inflammation and non-inflammatory states, and the interaction between the intestinal flora and external environmental factors.

[0003] When conducting testing, intestinal bacteria genetic testing equipment first needs to culture the collected intestinal bacteria samples to expand the sample capacity, then mix the flora samples evenly and divide them into multiple groups, and perform genetic testing on specific flora separately. However, existing testing equipment is unable to perform simultaneous testing on multiple flora samples, and complete testing is time-consuming and inefficient. Summary of the invention

[0004] The purpose of the present invention is to provide a kit for intestinal bacteria gene detection and a method of use to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A kit for intestinal bacteria gene detection comprises a detection box, wherein a base and a cover plate are arranged inside the detection box, a storage plate is fixedly installed in the middle of the detection box, a test tube rack is arranged at the center of the storage plate, the test tube rack is arranged in a circular shape, and detection test tubes are plugged in an annular distribution on the test tube rack, the test tube rack is connected with a space installation mechanism and a positioning mechanism, and a reagent filling mechanism and a centrifugal posture control mechanism are arranged on the storage plate.

[0007] The space installation mechanism includes a positioning ball 1 fixedly connected to the base, the center of the test tube rack is installed in cooperation with the outer ring of the positioning ball 1, the storage plate is annularly distributed with a plurality of arc frames at the edge of the test tube rack, an arc-shaped slide groove is arranged in the arc frame, a plurality of connecting curved rods are arranged at the bottom of the test tube rack corresponding to the arc frame, a matching ball is arranged at the end of the connecting curved rod, the matching ball is slidably installed with the arc-shaped slide groove, and a rotary drive component is arranged between the connecting curved rod and the test tube rack.

[0008] As a further solution of the present invention: the rotary drive assembly includes a concentric frame arranged at the bottom of the test tube rack, an annular clamping groove is arranged in the concentric frame, a matching pulley is arranged at one end of the connecting bent rod located in the annular clamping groove, the matching pulley and the annular clamping groove are slidingly fitted together, a mounting plate is arranged on the connecting bent rod, a driving motor is fixedly mounted on the mounting plate, the driving motor is connected to a matching gear 1, an inner ring of the concentric frame is provided with an inner gear ring, and the matching gear 1 and the inner gear ring are meshed with each other.

[0009] As a further solution of the present invention: the positioning mechanism includes an annular frame arranged on the storage plate, the annular frame is arranged concentrically with the test tube rack, the outer ring of the annular frame is provided with an outer gear ring, the storage plate is provided with a driving gear, the driving gear is connected to a power motor, the driving gear and the outer gear ring are meshed with each other, the storage plate is provided with a rotating frame distributed in an annular distribution on the inner ring part of the annular frame, the rotating frame is rotatably installed between the storage plate, the inner ring of the annular frame is rotatably installed with a connecting frame, a plug-in column is provided between the connecting frame and the rotating frame, and an end of the rotating frame away from the plug-in column points to the upper end surface of the test tube rack.

[0010] As a further solution of the present invention: a ball is provided at one end of the rotating frame facing the test tube rack, the ball is vertically plugged into the rotating frame, a tensioning spring is provided between the ball and the rotating frame, an annular groove is provided on the test tube rack, and the sizes of the ball and the annular groove match each other.

[0011] As a further solution of the present invention: the reagent filling mechanism includes a right-angle frame arranged on a storage plate, a reagent cartridge is arranged on the right-angle frame, a filling needle is lifted and lowered on the reagent cartridge, an adjustment groove is arranged on the right-angle frame, a matching slider is slidably installed in the adjustment groove, a telescopic motor is arranged on the right-angle frame, the telescopic motor is connected to the matching slider, a connecting column is arranged at the bottom of the right-angle frame, a sliding frame is slidably installed on the connecting column, a push-pull rod is rotatably installed between the end of the sliding frame and the matching slider, and the sliding frame is clamped with the filling needle.

[0012] As a further solution of the present invention: a mounting column is inserted on the sliding frame, a pressing frame is provided at the end of the mounting column, the pressing frame cooperates with the rubber plug on the test tube rack, and the part of the mounting column located between the sliding frame and the pressing frame is sleeved with a supporting spring.

[0013] As a further solution of the present invention: the centrifugal posture control mechanism includes a center rod arranged at the end of the right-angle frame, the end of the center rod is connected to a positioning ball 2, the centers of the positioning ball 2 and the positioning ball 1 are distributed on the same vertical axis, a cross is installed on the outer side of the positioning ball 2 for cooperation and rotation, the end of the right-angle frame is provided with a plurality of groups of electric telescopic rods distributed in a central ring around the center rod, the end of the electric telescopic rod is connected to a ball joint 1, a clamping ring frame is slidably installed on the cross, the ball joint 1 is fixedly connected to the clamping ring frame, a plurality of groups of ball joints 2 are arranged at the bottom of the cross, the ball joint 2 is connected to a connecting rod, the connecting rod is connected to a ball joint 3, and the ball joint 3 is distributed in a ring on the upper surface of the test tube rack.

[0014] A method for using the above-mentioned kit for intestinal bacteria gene detection comprises the following steps: S1, packing the cultured intestinal bacteria samples into multiple groups of detection test tubes, plugging the ports of the detection test tubes with rubber plugs, and distributing and installing the detection test tubes on a test tube rack; S2, positioning the test tube rack by a positioning mechanism, adding reagents to the detection test tubes on the test tube rack in combination with a reagent filling mechanism, and driving the test tube rack to rotate in combination with a rotary drive component to achieve sequential reagent filling of the detection test tubes; S3, the test tube rack filled with the detection reagent performs high-speed rotary centrifugal motion through the rotary drive component, and at the same time adjusts the pitch angle of the test tube rack in combination with a centrifugal posture control mechanism to drive the detection test tubes to fully centrifugally shake.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] (1) A plurality of arc-shaped racks form a spherical structure. The test tube rack is combined with a connecting curved rod and a matching ball to move in an arc-shaped slide groove, thereby realizing the free rotation of the test tube rack in space. When the test tube is filled with the test reagent, the arc-shaped rack is combined to effectively improve the mixing effect of the test tube in the test tube rack, thereby improving the reaction rate between the test reagent and the intestinal bacteria. By setting a concentric rack at the bottom of the test tube rack, in which the connecting curved rod is combined with a matching pulley and a circular clamping groove in the concentric rack for sliding installation, the test tube rack can be freely installed, and the rotary centrifugal drive of the test tube rack can be realized at the same time, and it is convenient to control the test tubes on the test tube rack to be filled with test reagents in sequence. By setting a mounting plate on the connecting curved rod, and then installing a drive motor and a matching gear 1, when the test tube rack rotates in space along the arc-shaped rack, the drive motor and the matching gear 1 can be combined at any position to drive the test tube rack to perform a rotary motion.

[0017] (2) An annular frame is arranged on the storage plate, and a driving gear arranged on the outer ring of the annular frame is meshed with the outer gear ring. When the annular frame rotates, the connecting frame and the rotating frame are driven to rotate, so that the end of the rotating frame is pressed against the upper end of the test tube rack, thereby keeping the test tube rack and the storage plate in a horizontal state, which is convenient for installing the test tube and filling the test tube with the test reagent.

[0018] (3) Before filling, the sliding frame first combines with the supporting spring to move the pressing frame downward and press on the rubber plug, and then the filling needle contacts and pierces the rubber plug to perform the filling operation. After the filling is completed, the sliding frame is retracted, and the filling needle is separated from the rubber plug first, and then the pressing frame is separated from the rubber plug, thereby preventing the rubber plug and the test tube from being separated when the filling needle is separated. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the external structure of the present invention.

[0020] Figure 2 It is a schematic diagram of the internal cutaway structure of the present invention.

[0021] Figure 3 It is a schematic diagram of the installation of the storage plate in the present invention.

[0022] Figure 4 It is a schematic diagram of the connection structure between the test tube rack and the storage plate in the present invention.

[0023] Figure 5 This is a schematic diagram of another viewing angle of the test tube rack and the storage plate in the present invention.

[0024] Figure 6 for Figure 5 Enlarged structural diagram at A in the middle.

[0025] Figure 7 It is a schematic diagram of the connection between the positioning mechanism and the storage plate in the present invention.

[0026] Figure 8 for Figure 7 Enlarged structural diagram at B in the middle.

[0027] Fig. 9 It is a schematic diagram of the installation of the reagent filling mechanism and the centrifugal posture control mechanism in the present invention.

[0028] Fig.10 It is a structural schematic diagram of the reagent filling mechanism in the present invention.

[0029] Fig.11 It is a structural schematic diagram of the centrifugal posture control mechanism in the present invention.

[0030] In the figure: 1, test box; 10, cover plate; 11, bottom bracket; 2, storage plate; 20, test tube; 21, test tube rack; 211, annular groove; 3, space installation mechanism; 30, positioning ball 1; 31, arc frame; 310, arc slide; 32, concentric frame; 320, inner gear ring; 33, annular clamping groove; 34, matching pulley; 35, connecting curved rod; 36, matching ball; 37, mounting plate; 38, driving motor; 39, matching gear 1; 4, positioning mechanism; 40, annular frame; 41, outer gear ring; 42, driving gear; 43, rotating frame; 44, connecting frame; 45. Plug-in column; 46. Ball bearing; 47. Tension spring; 5. Reagent filling mechanism; 50. Right-angle frame; 51. Reagent cylinder; 52. Filling needle; 53. Telescopic motor; 54. Adjustment slot; 55. Matching slider; 56. Push-pull rod; 57. Connecting column; 58. Sliding frame; 59. Mounting column; 510. Support spring; 511. Press frame; 6. Centrifugal posture control mechanism; 60. Center rod; 61. Positioning ball 2; 62. Cross; 63. Snap-on ring frame; 64. Ball joint 1; 65. Electric telescopic rod; 66. Ball joint 2; 67. Ball joint 3; 68. Connecting rod. DETAILED DESCRIPTION

[0031] The technical solution of the present invention is further described in detail below in conjunction with specific implementation methods.

[0032] like Figure 1 , Figure 2 , Figure 3 As shown, a kit for intestinal bacteria gene detection includes a detection box 1, wherein a base 11 and a cover 10 are arranged in the detection box 1, a storage plate 2 is fixedly installed in the middle of the detection box 1, a test tube rack 21 is arranged at the center of the storage plate 2, the test tube rack 21 is arranged in a circular shape, and detection test tubes 20 are inserted in a ring-shaped distribution on the test tube rack 21, the test tube rack 21 is connected with a space installation mechanism 3 and a positioning mechanism 4, and a reagent filling mechanism 5 and a centrifugal posture control mechanism 6 are arranged on the storage plate 2.

[0033] like Figure 4 , Figure 5 , Figure 6 As shown, the space installation mechanism 3 includes a positioning ball 30 fixedly connected to the base 11, the center of the test tube rack 21 is mounted in cooperation with the outer ring of the positioning ball 30, the storage plate 2 is annularly distributed with a plurality of arc frames 31 at the edge of the cooperation with the test tube rack 21, an arc-shaped slide groove 310 is arranged in the arc frame 31, a plurality of connecting curved rods 35 are arranged at the bottom of the test tube rack 21 corresponding to the arc frame 31, a matching ball 36 is arranged at the end of the connecting curved rod 35, the matching ball 36 is slidably installed with the arc-shaped slide groove 310, and a rotary drive component is arranged between the connecting curved rod 35 and the test tube rack 21.

[0034] Specifically, a plurality of arc-shaped racks 31 form a spherical structure, and the test tube rack 21 moves in the arc-shaped slide groove 310 in combination with the connecting curved rod 35 and the matching ball 36, thereby realizing the free rotation of the test tube rack 21 in space. When the test tube 20 is filled with the test reagent, the arc-shaped rack 31 is combined to effectively improve the mixing effect of the test tube 20 in the test tube rack 21, thereby improving the reaction rate between the test reagent and the intestinal bacteria.

[0035] Further, such as Figure 6 As shown, the rotary drive assembly includes a concentric frame 32 arranged at the bottom of the test tube rack 21, and an annular clamping groove 33 is arranged in the concentric frame 32. A matching pulley 34 is arranged at one end of the connecting bent rod 35 located in the annular clamping groove 33. The matching pulley 34 is slidably mounted with the annular clamping groove 33. A mounting plate 37 is arranged on the connecting bent rod 35, and a driving motor 38 is fixedly mounted on the mounting plate 37. The driving motor 38 is connected to a matching gear 1 39. The inner ring of the concentric frame 32 is provided with an inner gear ring 320, and the matching gear 1 39 and the inner gear ring 320 are meshed with each other.

[0036] Specifically, by arranging a concentric frame 32 at the bottom of the test tube rack 21, wherein the connecting curved rod 35 is slidably installed in combination with the matching pulley 34 and the annular clamping groove 33 in the concentric frame 32, the test tube rack 21 can be freely installed, and the rotary centrifugal drive of the test tube rack 21 can be realized at the same time, and it is convenient to control the test tubes 20 on the test tube rack 21 to be filled with test reagents in sequence. By arranging a mounting plate 37 on the connecting curved rod 35, and then installing a driving motor 38 and a matching gear 1 39, when the test tube rack 21 rotates in space along the arc frame 31, the test tube rack 21 can be driven to rotate at any position in combination with the driving motor 38 and the matching gear 1 39.

[0037] Further, such as Figure 7 , Figure 8 As shown, the positioning mechanism 4 includes an annular frame 40 arranged on the storage plate 2, the annular frame 40 is concentrically arranged with the test tube rack 21, the outer ring of the annular frame 40 is provided with an outer gear ring 41, a driving gear 42 is provided on the storage plate 2, the driving gear 42 is connected to a power motor, the driving gear 42 and the outer gear ring 41 are meshed with each other, the storage plate 2 is provided with a rotating frame 43 distributed in an annular manner on the inner ring part of the annular frame 40, the rotating frame 43 is rotatably installed between the storage plate 2, the inner ring of the annular frame 40 is rotatably installed with a connecting frame 44, a plug-in column 45 is provided between the connecting frame 44 and the rotating frame 43, and the end of the rotating frame 43 away from the plug-in column 45 points to the upper end surface of the test tube rack 21.

[0038] Specifically, the test tube rack 21 needs to be kept horizontal when installing the test tube 20 and filling the test reagent. By setting an annular rack 40 on the storage plate 2, the driving gear 42 set on the outer ring of the annular rack 40 is engaged with the outer gear ring 41. When the annular rack 40 rotates, it drives the connecting rack 44 and the rotating rack 43 to rotate, so that the end of the rotating rack 43 is pressed on the upper end of the test tube rack 21, so that the test tube rack 21 and the storage plate 2 are kept horizontal, which is convenient for installing the test tube 20 and filling the test reagent into the test tube 20.

[0039] Further, such as Figure 8 As shown, a ball 46 is provided at one end of the rotating frame 43 facing the test tube rack 21, and the ball 46 is vertically inserted into the rotating frame 43. A tensioning spring 47 is provided between the ball 46 and the rotating frame 43. An annular groove 211 is provided on the test tube rack 21, and the sizes of the ball 46 and the annular groove 211 match each other.

[0040] Specifically, in order to reduce the resistance encountered by the test tube rack 21 during the rotational movement when the rotating frame 43 moves to the upper surface of the test tube rack 21, a ball 46 is provided at the end of the rotating frame 43 and is tensioned and installed in combination with a tensioning spring 47. When the ball 46 moves with the rotating frame 43 and falls into the annular groove 211, the test tube rack 21 is horizontally positioned. At the same time, the resistance between the test tube rack 21 and the ball 46 is greatly reduced when the test tube rack 21 rotates driven by the rotary drive assembly.

[0041] Further, such as Fig. 9 , Fig.10 As shown, the reagent filling mechanism 5 includes a right-angle frame 50 arranged on the storage plate 2, a reagent cartridge 51 is arranged on the right-angle frame 50, a filling needle 52 is installed on the reagent cartridge 51 for lifting, an adjustment slot 54 is arranged on the right-angle frame 50, a matching slider 55 is slidably installed in the adjustment slot 54, a telescopic motor 53 is arranged on the right-angle frame 50, and the telescopic motor 53 is connected to the matching slider 55, a connecting column 57 is arranged at the bottom of the right-angle frame 50, a sliding frame 58 is slidably installed on the connecting column 57, a push-pull rod 56 is rotatably installed between the end of the sliding frame 58 and the matching slider 55, and the sliding frame 58 is clamped with the filling needle 52.

[0042] Specifically, when the test tube 20 rotates to the bottom of the reagent filling mechanism 5 along with the test tube rack 21, the telescopic motor 53 controls the sliding block 55 to move forward, and the push-pull rod 56 is combined to make the sliding frame 58 move downward along the connecting column 57, thereby driving the filling needle 52 to move downward, piercing the rubber stopper on the test tube 20, and filling the test tube 20 with the reagent.

[0043] Further, such as Fig.10As shown, a mounting column 59 is inserted into the sliding frame 58, and a pressing frame 511 is provided at the end of the mounting column 59. The pressing frame 511 cooperates with the rubber plug on the test tube rack 21, and the part of the mounting column 59 located between the sliding frame 58 and the pressing frame 511 is sleeved with a supporting spring 510.

[0044] Specifically, in order to ensure that the rubber stopper will not be detached from the end of the inspection test tube 20 before and after filling, before filling, the sliding frame 58 is first combined with the supporting spring 510 to move the pressing frame 511 downward to press on the rubber stopper, and then the filling needle 52 contacts and pierces the rubber stopper to perform the filling operation. After the filling is completed, the sliding frame 58 is retracted, and the filling needle 52 is detached before the rubber stopper, and then the pressing frame 511 is detached from the rubber stopper, thereby avoiding the rubber stopper and the inspection test tube 20 being separated when the filling needle 52 is detached.

[0045] Further, such as Fig.11 As shown, the centrifugal posture control mechanism 6 includes a center rod 60 arranged at the end of the right-angle frame 50, and the end of the center rod 60 is connected to a positioning ball 2 61, and the positioning ball 2 61 and the positioning ball 1 30 are distributed on the same vertical axis. A cross 62 is installed on the outer side of the positioning ball 2 61 for rotation. The end of the right-angle frame 50 is annularly distributed around the center rod 60 as the center, and the end of the electric telescopic rod 65 is connected to a ball joint 1 64. A snap-on ring frame 63 is slidably installed on the cross 62, and the ball joint 1 64 is fixedly connected to the snap-on ring frame 63. A plurality of ball joints 2 66 are arranged at the bottom of the cross 62, and the ball joint 2 66 is connected to a connecting rod 68, and the connecting rod 68 is connected to a ball joint 3 67. The ball joint 3 67 is annularly distributed and installed on the upper surface of the test tube rack 21.

[0046] Specifically, in order to flexibly control the rotary centrifugal posture of the test tube rack 21, the clamping ring rack 63 is installed through multiple sets of electric telescopic rods 65 and ball joints 1 64, and the lengths of the electric telescopic rods 65 are controlled respectively to adjust the inclination angle of the clamping ring rack 63, thereby adjusting the inclination angle of the cross 62. The cross 62 is connected to the test tube rack 21 through the ball joint 2 66, the connecting rod 68 and the ball joint 3 67, thereby driving the test tube rack 21 to tilt synchronously. Combined with the rotary drive assembly, the test tube rack 21 performs centrifugal movement at a specific inclination angle, driving the test tube 20 to be fully mixed with the detection reagent.

[0047] A method for using the above-mentioned kit for intestinal bacteria gene detection includes the following steps: S1, packing the cultured intestinal bacteria samples into multiple groups of detection test tubes 20, plugging the ports of the detection test tubes 20 with rubber plugs, and distributing and installing the detection test tubes 20 on a test tube rack 21; S2, positioning the test tube rack 21 through a positioning mechanism 4, adding reagents to the detection test tubes 20 on the test tube rack 21 in combination with a reagent filling mechanism 5, and driving the test tube rack 21 to rotate in combination with a rotary drive component to achieve sequential reagent filling of the detection test tubes 20; S3, the test tube rack 21 filled with the detection reagent performs high-speed rotary centrifugal motion through a rotary drive component, and at the same time adjusts the pitch angle of the test tube rack 21 in combination with a centrifugal posture control mechanism 6, driving the detection test tubes 20 to fully centrifugally shake.

[0048] The working principle of the embodiment of the present invention is:

[0049] like Figure 1-Figure 11As shown, a plurality of arc-shaped racks 31 form a spherical structure, and the test tube rack 21 moves in the arc-shaped slide groove 310 in combination with the connecting curved rod 35 and the matching ball 36, thereby realizing the free rotation of the test tube rack 21 in space. When the test tube 20 is filled with the detection reagent, the arc-shaped rack 31 is combined to effectively improve the mixing effect of the test tube 20 in the test tube rack 21, thereby improving the reaction rate between the detection reagent and the intestinal bacteria. By setting a concentric rack 32 at the bottom of the test tube rack 21, wherein the connecting curved rod 35 is combined with the matching pulley 34 and the annular clamping groove 33 in the concentric rack 32 for sliding installation, the test tube rack 21 is free to be installed, and the rotary centrifugal drive of the test tube rack 21 can be realized at the same time, and it is convenient to control the test tubes 20 on the test tube rack 21 to be filled with the detection reagent in sequence. By setting a mounting plate 37 on the connecting curved rod 35, and then installing a driving motor 38 and a matching gear 39, when the test tube rack 21 rotates in space along the arc frame 31, the driving motor 38 and the matching gear 39 can be combined at any position to drive the test tube rack 21 to perform a rotary motion. When installing the test tube 20 and filling the test reagent, the test tube rack 21 needs to be kept in a horizontal state. By setting an annular frame 40 on the storage plate 2, the driving gear 42 set on the outer ring of the annular frame 40 is meshed with the outer gear ring 41. When the annular frame 40 rotates, it drives the connecting frame 44 and the rotating frame 43 to rotate, so that the end of the rotating frame 43 is pressed on the upper end of the test tube rack 21, so that the test tube rack 21 and the storage plate 2 are kept in a horizontal state, which is convenient for installing the test tube 20 and filling the test reagent on the test tube 20. In order to reduce the resistance of the test tube rack 21 during the rotation when the rotating rack 43 moves to the upper surface of the test tube rack 21, a ball 46 is arranged at the end of the rotating rack 43 and is tensioned and installed in combination with a tension spring 47. When the ball 46 moves with the rotating rack 43 and falls into the annular groove 211, it plays a role in horizontal positioning of the test tube rack 21. At the same time, the resistance between the test tube rack 21 and the ball 46 is greatly reduced when the test tube rack 21 rotates under the drive of the rotary drive assembly. When the test tube 20 rotates to the bottom of the reagent filling mechanism 5 with the test tube rack 21, the telescopic motor 53 controls the sliding block 55 to move forward, and the push-pull rod 56 is combined to make the sliding frame 58 move down along the connecting column 57, thereby driving the filling needle 52 to move downward, piercing the rubber stopper on the test tube 20, and filling the test tube 20 with the reagent. In order to ensure that the rubber stopper will not be separated from the end of the inspection test tube 20 before and after filling, before filling, the sliding frame 58 is first combined with the supporting spring 510 to move the pressing frame 511 downward to press on the rubber stopper, and then the filling needle 52 contacts and pierces the rubber stopper to perform the filling operation. After the filling is completed, the sliding frame 58 is retracted, and the filling needle 52 is separated from the rubber stopper first, and then the pressing frame 511 is separated from the rubber stopper, thereby avoiding the rubber stopper and the inspection test tube 20 being separated when the filling needle 52 is separated.

[0050] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modification, equivalent substitution, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention. Any reference numeral in a claim shall not be regarded as limiting the claim involved.

[0051] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A kit for intestinal bacteria gene detection, comprising a detection box (1), wherein a base (11) and a cover plate (10) are arranged in the detection box (1), a storage plate (2) is fixedly installed in the middle of the detection box (1), a test tube rack (21) is arranged in the center of the storage plate (2), the test tube rack (21) is arranged in a circular shape, and detection test tubes (20) are inserted in an annular distribution on the test tube rack (21), characterized in that: The test tube rack (21) is connected to a space installation mechanism (3) and a positioning mechanism (4), and the storage plate (2) is provided with a reagent filling mechanism (5) and a centrifugal posture control mechanism (6); The spatial mounting mechanism (3) comprises a positioning ball (30) fixedly connected to the base (11); the center of the test tube rack (21) is mounted in cooperation with the outer ring of the positioning ball (30); the storage plate (2) is provided with a plurality of arc frames (31) distributed in an annular manner at the edge of the test tube rack (21); an arc-shaped slide groove (310) is arranged in the arc frame (31); a plurality of connecting curved rods (35) are arranged at the bottom of the test tube rack (21) corresponding to the arc frame (31); a matching ball (36) is arranged at the end of the connecting curved rod (35); the matching ball (36) is slidably mounted between the arc-shaped slide groove (310); and a rotary drive component is arranged between the connecting curved rod (35) and the test tube rack (21).

2. A kit for intestinal bacteria gene detection according to claim 1, characterized in that: The rotary drive assembly comprises a concentric frame (32) arranged at the bottom of the test tube rack (21), an annular clamping groove (33) is arranged in the concentric frame (32), a matching pulley (34) is arranged at one end of the connecting bent rod (35) located in the annular clamping groove (33), the matching pulley (34) and the annular clamping groove (33) are slidably mounted, a mounting plate (37) is arranged on the connecting bent rod (35), a driving motor (38) is fixedly mounted on the mounting plate (37), the driving motor (38) is connected to a matching gear 1 (39), an inner ring of the concentric frame (32) is provided with an inner gear ring (320), and the matching gear 1 (39) and the inner gear ring (320) are meshed with each other.

3. A kit for intestinal bacteria gene detection according to claim 1, characterized in that: The positioning mechanism (4) comprises an annular frame (40) arranged on the receiving plate (2), the annular frame (40) being arranged concentrically with the test tube rack (21), an outer ring of the annular frame (40) being provided with an outer gear ring (41), a driving gear (42) being provided on the receiving plate (2), the driving gear (42) being connected with a power motor, the driving gear (42) and the outer gear ring (41) being meshed with each other, a rotating frame (43) being arranged in an annular distribution on the inner ring of the annular frame (40) of the receiving plate (2), the rotating frame (43) being rotatably mounted between the receiving plate (2), a connecting frame (44) being rotatably mounted on the inner ring of the annular frame (40), a plug-in column (45) being provided between the connecting frame (44) and the rotating frame (43), and an end of the rotating frame (43) away from the plug-in column (45) pointing to the upper end surface of the test tube rack (21).

4. A kit for intestinal bacteria gene detection according to claim 3, characterized in that: A ball (46) is arranged at one end of the rotating frame (43) facing the test tube rack (21); the ball (46) and the rotating frame (43) are vertically plugged together; a tension spring (47) is arranged between the ball (46) and the rotating frame (43); an annular groove (211) is arranged on the test tube rack (21); and the sizes of the ball (46) and the annular groove (211) match each other.

5. A kit for intestinal bacteria gene detection according to claim 4, characterized in that: The reagent filling mechanism (5) comprises a right-angle frame (50) arranged on the storage plate (2), a reagent cartridge (51) being arranged on the right-angle frame (50), a filling needle (52) being installed in a lifting manner on the reagent cartridge (51), an adjustment groove (54) being arranged on the right-angle frame (50), a matching slider (55) being slidably installed in the adjustment groove (54), a telescopic motor (53) being arranged on the right-angle frame (50), the telescopic motor (53) being connected to the matching slider (55), a connecting column (57) being arranged at the bottom of the right-angle frame (50), a sliding frame (58) being slidably installed on the connecting column (57), a push-pull rod (56) being rotatably installed between the end of the sliding frame (58) and the matching slider (55), and the sliding frame (58) being clamped with the filling needle (52).

6. A kit for intestinal bacteria gene detection according to claim 5, characterized in that: The sliding frame (58) is plugged with a mounting post (59), and a pressing frame (511) is provided at the end of the mounting post (59). The pressing frame (511) cooperates with a rubber plug on the test tube rack (21), and a support spring (510) is sleeved on the portion of the mounting post (59) located between the sliding frame (58) and the pressing frame (511).

7. A kit for intestinal bacteria gene detection according to claim 6, characterized in that: The centrifugal attitude control mechanism (6) comprises a central rod (60) arranged at the end of a right-angle frame (50), the end of the central rod (60) is connected to a second positioning ball (61), the center of the second positioning ball (61) and the center of the first positioning ball (30) are distributed on the same vertical axis, a cross (62) is mounted on the outer side of the second positioning ball (61) for rotation, and a plurality of groups of electric telescopic rods (65) are arranged in a central annular distribution around the central rod (60) at the end of the right-angle frame (50). The end of the electric telescopic rod (65) is connected to a ball joint 1 (64), a clamping ring frame (63) is slidably mounted on the cross (62), the ball joint 1 (64) and the clamping ring frame (63) are fixedly connected, and a plurality of groups of ball joints 2 (66) are arranged at the bottom of the cross (62), the ball joints 2 (66) are connected to a connecting rod (68), the connecting rod (68) is connected to a ball joint 3 (67), and the ball joints 3 (67) are annularly distributed and mounted on the upper surface of the test tube rack (21).

8. A method for using the kit for intestinal bacteria gene detection as claimed in claim 7, characterized in that: The following steps are involved: S1. Packing the cultured intestinal bacteria samples into a plurality of groups of test tubes (20), plugging the ports of the test tubes (20) with rubber stoppers, and distributing and installing the test tubes (20) on a test tube rack (21); S2, positioning the test tube rack (21) by means of the positioning mechanism (4), adding reagents to the test tubes (20) on the test tube rack (21) in combination with the reagent filling mechanism (5), and driving the test tube rack (21) to rotate in combination with the rotary drive assembly, so as to sequentially add reagents to the test tubes (20); S3. The test tube rack (21) filled with the detection reagent is subjected to high-speed rotary centrifugal motion through the rotary drive assembly, and the pitch angle of the test tube rack (21) is adjusted in combination with the centrifugal attitude control mechanism (6), so as to drive the detection test tube (20) to fully shake centrifugally.

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