Interplant mycorrhizal nutrient transfer culture equipment and method

Abstract

The invention provides inter-plant mycorrhiza nutrient transfer culture equipment which comprises a culture box body and an organic glass plate, an inner cavity of the culture box body is divided into a first culture chamber and a second culture chamber through the organic glass plate, the organic glass plate is a porous glass plate, and a white nylon net is pasted on one side of the porous glass plate. And the white nylon net is divided into a white nylon net with the aperture of 0.45 mu m and a white nylon net with the aperture of 37 mu m. The equipment is small in size, small in occupied area, cheap in material, convenient to assemble and suitable for pot culture experiments of large-batch samples, soil ions are allowed to pass through by means of 0.45 microns, ectogenic hyphae and the soil ions are allowed to pass through by means of a white nylon net of 37 microns, the soil ions, the ectogenic hyphae and root systems are allowed to pass through a nylon-free net, and the soil ions, the ectogenic hyphae and the root systems are allowed to pass through. Compared with the prior art, direct and indirect paths of nutrient transfer between plants can be distinguished and quantified, and the method is simple and easy to implement.

Description

Technical Field

[0001] The invention belongs to the technical field of mycorrhizal nutrient transfer cultivation, and in particular relates to an inter-plant mycorrhizal nutrient transfer cultivation device and method. Background Art

[0002] Mycorrhizae are symbiotic associations with specific morphology and function formed by fungi and plant roots. About 90% of terrestrial plants form symbiotic relationships with mycorrhizal fungi. Mycorrhizal fungi provide resources to trees, especially carbon and nitrogen, and can improve the resistance of their hosts to abiotic and biotic stresses, while the fungi receive a portion of the fixed carbon from the trees in return. This association is particularly beneficial for trees growing in harsh environments or degraded sites. The symbiotic mycorrhizal network formed by interconnected hyphae enables resource redistribution and sharing among trees. This exchange of materials and information among trees has far-reaching implications for plant community dynamics, ecosystem productivity, diversity and stability.

[0003] There are mainly the following ways for nutrient transfer between two or more plants:

[0004] 1. Direct transfer through the mycorrhizal network (mycorrhizal pathway);

[0005] 2. Aseptic root fungi participate in the indirect transfer of "root exudates-soil" (root pathway);

[0006] 3. Indirect transfer involving aseptic root fungi and root systems (soil pathway).

[0007] Existing technologies mainly focus on nutrient transfer between nitrogen-fixing and non-nitrogen-fixing plants and between plants with different mycorrhizal types, especially the mycorrhizal pathway of nutrient transfer, but do not distinguish between the direct and indirect effects of mycorrhizae, roots and soil pathways on nutrient transfer.

[0008] Based on this, a plant mycorrhizal nutrient transfer cultivation device and method are proposed. Summary of the invention

[0009] The technical problem to be solved by the present invention is to provide a plant mycorrhizal nutrient transfer and cultivation device and method in view of the deficiencies of the above-mentioned prior art, so as to solve the problems raised in the above-mentioned background technology.

[0010] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0011] In a first aspect, a plant mycorrhizal nutrient transfer culture device comprises a culture box and a plexiglass plate;

[0012] The culture box is a rectangular box structure with an open top, and a plexiglass plate is fixed in the middle of the culture box. The internal cavity of the culture box is divided into a first culture chamber and a second culture chamber by the plexiglass plate. Drainage holes are provided on the ground inside the first culture chamber and the second culture chamber of the culture box.

[0013] As a further illustration of the present invention, the dimensions of the culture box are 40 cm long×20 cm wide×30 cm high, and 2.5 mm thick, and the dimensions of the organic glass plate are 20 cm long×30 cm wide, and 2.5 mm thick.

[0014] As a further illustration of the present invention, the organic glass plate is a perforated glass plate, the aperture of the holes is 6 mm, the aperture spacing is 1 cm, and a white nylon mesh is pasted on one side of the perforated glass plate.

[0015] As a further illustration of the present invention, the white nylon mesh is divided into a white nylon mesh with a pore size of 0.45 μm and a white nylon mesh with a pore size of 37 μm. The white nylon mesh with a pore size of 0.45 μm only allows soil solution to pass through, but does not allow hyphae and roots to pass through, so as to study the net nutrient transfer between plants produced through the soil pathway; the white nylon mesh with a pore size of 37 μm only allows hyphae and soil solution to pass through, but does not allow roots to pass through, so as to study the net nutrient transfer between plants produced through the mycorrhiza and soil pathways.

[0016] In a second aspect, a method for cultivating a plant mycorrhizal nutrient transfer device comprises the following steps:

[0017] Soil pathways for nutrient transfer between plants are used by:

[0018] S101, select a plexiglass plate as a porous glass plate, and paste a white nylon mesh with a pore size of 0.45 μm on the porous glass plate, then respectively load the first culture chamber and the second culture chamber with the same volume of a mixed matrix of forest topsoil and perlite containing ectomycorrhizal fungi, and transplant one plant in each of the first culture chamber and the second culture chamber, the plant on the side pasted with the white nylon mesh with a pore size of 0.45 μm is labeled with carbon and nitrogen isotopes, and is referred to as the donor plant, and the other plant is not labeled with carbon and nitrogen isotopes, and is referred to as the recipient plant;

[0019] S102. After 2-3 months of transplanting, after successful inoculation of ectomycorrhizal fungi, the donor plants are labeled with carbon and nitrogen isotopes using 99.7atom% urea and 99atom% 13 CO2;

[0020] S103. Before labeling and 6-12 months after labeling, investigate the ectomycorrhizal infection rate, aboveground and underground biomass, total C% and atom% of donor and recipient plants. 13C, full N% and atom% 15 N, calculates the carbon transfer rate and nitrogen transfer rate between plants through soil pathways;

[0021] Soil pathways for nutrient transfer between plants are used by:

[0022] S201, select the organic glass plate as the porous glass plate, and paste the white nylon mesh with a pore size of 37 μm on the porous glass plate, and then respectively load the first culture chamber and the second culture chamber with the same volume of the mixed matrix of forest topsoil and perlite containing ectomycorrhizal fungi, and transplant one plant in each of the first culture chamber and the second culture chamber, the plant on the side pasted with the white nylon mesh with a pore size of 37 μm is labeled with carbon and nitrogen isotopes, and is called the donor plant, and the other plant is not labeled with carbon and nitrogen isotopes, and is called the recipient plant;

[0023] S202. After 2-3 months of transplanting, after successful inoculation of ectomycorrhizal fungi, the donor plants are labeled with carbon and nitrogen isotopes using 99.7atom% urea and 99atom% 13 CO2;

[0024] S203. Before labeling and 6-12 months after labeling, investigate the ectomycorrhizal infection rate, aboveground and underground biomass, total C% and atom% of donor and recipient plants. 13 C. Full N% and atom% 15 N, calculate the carbon transfer rate and nitrogen transfer rate between plants produced by mycorrhiza and root pathways;

[0025] The mycorrhizae, root systems and soil pathways of nutrient transfer between plants are used to:

[0026] S301. There is no organic glass plate in the middle of the culture box, allowing hyphae, roots and soil ions to pass through; transplant a plant on each side of the culture box, one of which is labeled with carbon and nitrogen isotopes and is the donor plant, and the other plant is not labeled with carbon and nitrogen isotopes and is the recipient plant;

[0027] S302. 2-3 months after transplanting, after successful inoculation of ectomycorrhizal fungi, the donor plants are labeled with carbon and nitrogen isotopes using 99.7atom% urea and 99atom% 13 CO2;

[0028] S303. Before and 6-12 months after labeling, investigate the ectomycorrhizal infection rate, aboveground and underground biomass, total C% and atom% of donor and recipient plants. 13 C. Full N% and atom% 15 N, calculate the carbon transfer rate and nitrogen transfer rate between plants produced by mycorrhizal and root pathways.

[0029] As a further illustration of the present invention, the depth of the forest topsoil with ectomycorrhizal fungi is 10-20 cm, and the volume ratio of the forest topsoil with ectomycorrhizal fungi to perlite in the mixed matrix is ​​2:1.

[0030] Compared with the prior art, the present invention has the following advantages:

[0031] 1. The device of the present invention is small in size, occupies a small area, is made of cheap materials, is easy to assemble, and is suitable for potted plant experiments with large quantities of samples.

[0032] 2. The present invention collects the surface soil under the forest as the inoculation source of ectomycorrhizal fungi. The mycorrhizal fungi species are richer and closer to the mycorrhizal fungal community under natural conditions. It can better reflect the inoculation state of plants under natural conditions than the mycorrhizal fungi infection in the prior art, and the nutrient transfer results between plants are more real and reliable.

[0033] 3. The present invention utilizes 0.45 μm to allow soil ions to pass through, 37 μm white nylon mesh to allow exogenous hyphae and soil ions to pass through, and no nylon mesh to allow soil ions, exogenous hyphae and roots to pass through. Compared with the existing technology, the present invention is more capable of distinguishing and quantifying the direct and indirect pathways of nutrient transfer between plants, and the method is simple and easy. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0035] Description of reference numerals:

[0036] 1-culture box; 2-plexiglass plate; 11-first culture chamber; 12-second culture chamber; 13-drainage hole. DETAILED DESCRIPTION

[0037] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0038] like Figure 1 As shown, the present invention provides a technical solution: a plant mycorrhizal nutrient transfer culture device, comprising a culture box 1 and a plexiglass plate 2;

[0039] The culture box 1 is a rectangular box structure with an open top, and a plexiglass plate 2 is fixed in the middle of the culture box 1. The dimensions of the culture box 1 are 40 cm long × 20 cm wide × 30 cm high, and the thickness is 2.5 mm. The dimensions of the plexiglass plate 2 are 20 cm long × 30 cm wide, and the thickness is 2.5 mm.

[0040] The inner cavity of the culture box 1 is divided into a first culture chamber 11 and a second culture chamber 12 by an organic glass plate 2 . Drain holes 13 are arranged on the ground of the first culture chamber 11 and the second culture chamber 12 in the culture box 1 .

[0041] The organic glass plate 2 is a porous glass plate with a hole diameter of 6 mm and a hole diameter spacing of 1 cm, and a white nylon mesh is pasted on one side of the porous glass plate.

[0042] The white nylon mesh is divided into a white nylon mesh with a pore size of 0.45 μm and a white nylon mesh with a pore size of 37 μm. The white nylon mesh with a pore size of 0.45 μm only allows soil solution to pass through, but does not allow hyphae and root systems to pass through, so as to study the net nutrient transfer between plants produced through the soil pathway; the white nylon mesh with a pore size of 37 μm only allows hyphae and soil solution to pass through, but does not allow root systems to pass through, so as to study the net nutrient transfer between plants produced through the mycorrhiza and soil pathways.

[0043] The above-mentioned method of the interplant mycorrhizal nutrient transfer cultivation device comprises the following steps:

[0044] Soil pathways for nutrient transfer between plants are used by:

[0045] S101, select the organic glass plate 2 as a porous glass plate, and paste a white nylon mesh with a pore size of 0.45 μm on the porous glass plate, and then respectively load the first culture chamber 11 and the second culture chamber 12 with the same volume of a mixed matrix of forest topsoil and perlite containing ectomycorrhizal fungi, and transplant one plant in each of the first culture chamber 11 and the second culture chamber 12, the plant on the side pasted with the white nylon mesh with a pore size of 0.45 μm is labeled with carbon and nitrogen isotopes, and is called the donor plant, and the other plant is not labeled with carbon and nitrogen isotopes, and is called the recipient plant;

[0046] S102. After 2-3 months of transplanting, after successful inoculation of ectomycorrhizal fungi, the donor plants are labeled with carbon and nitrogen isotopes using 99.7atom% urea and 99atom% 13 CO2;

[0047] S103. Before labeling and 6-12 months after labeling, investigate the ectomycorrhizal infection rate, aboveground and underground biomass, total C% and atom% of donor and recipient plants. 13 C, full N% and atom% 15N, calculates the carbon transfer rate and nitrogen transfer rate between plants through soil pathways;

[0048] Soil pathways for nutrient transfer between plants are used by:

[0049] S201, select the organic glass plate 2 as a porous glass plate, and paste a white nylon mesh with a pore size of 37 μm on the porous glass plate, and then respectively load the first culture chamber 11 and the second culture chamber 12 with the same volume of a mixed matrix of forest topsoil and perlite containing ectomycorrhizal fungi, and transplant one plant in each of the first culture chamber 11 and the second culture chamber 12, the plant on the side pasted with the white nylon mesh with a pore size of 37 μm is labeled with carbon and nitrogen isotopes, and is called the donor plant, and the other plant is not labeled with carbon and nitrogen isotopes, and is called the recipient plant;

[0050] S202. After 2-3 months of transplanting, after successful inoculation of ectomycorrhizal fungi, the donor plants are labeled with carbon and nitrogen isotopes using 99.7atom% urea and 99atom% 13 CO2;

[0051] S203. Before labeling and 6-12 months after labeling, investigate the ectomycorrhizal infection rate, aboveground and underground biomass, total C% and atom% of donor and recipient plants. 13 C. Full N% and atom% 15 N, calculate the carbon transfer rate and nitrogen transfer rate between plants produced by mycorrhiza and root pathways;

[0052] The mycorrhizae, root systems and soil pathways of nutrient transfer between plants are used to:

[0053] S301, there is no organic glass plate 2 in the middle of the culture box 1, allowing hyphae, roots and soil ions to pass through; transplanting a plant on each side of the culture box 1, one of which is labeled with carbon and nitrogen isotopes and is the donor plant, and the other plant is not labeled with carbon and nitrogen isotopes and is the recipient plant;

[0054] S302. 2-3 months after transplanting, after successful inoculation of ectomycorrhizal fungi, the donor plants are labeled with carbon and nitrogen isotopes using 99.7atom% urea and 99atom% 13 CO2;

[0055] S303. Before and 6-12 months after labeling, investigate the ectomycorrhizal infection rate, aboveground and underground biomass, total C% and atom% of donor and recipient plants. 13 C. Full N% and atom% 15 N, calculate the carbon transfer rate and nitrogen transfer rate between plants produced by mycorrhizal and root pathways.

[0056] The depth of the forest topsoil with ectomycorrhizal fungi is 10-20 cm, and the volume ratio of the forest topsoil with ectomycorrhizal fungi to perlite in the mixed matrix is ​​2:1.

[0057] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0058] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A plant mycorrhizal nutrient transfer and cultivation device, characterized in that: It comprises a culture box body (1) and a plexiglass plate (2); The culture box (1) is a rectangular box structure with an open top, and a plexiglass plate (2) is fixed in the middle of the culture box (1), and the inner cavity of the culture box (1) is divided into a first culture chamber (11) and a second culture chamber (12) by the plexiglass plate (2), and drainage holes (13) are arranged on the ground inside the first culture chamber (11) and the second culture chamber (12) in the culture box (1).

2. The interplant mycorrhizal nutrient transfer and cultivation device according to claim 1, characterized in that: The dimensions of the culture box (1) are 40 cm long×20 cm wide×30 cm high, and 2.5 mm thick; the dimensions of the organic glass plate (2) are 20 cm long×30 cm wide, and 2.5 mm thick.

3. The interplant mycorrhizal nutrient transfer and cultivation device according to claim 1, characterized in that: The organic glass plate (2) is a perforated glass plate, the aperture of the holes is 6 mm, and the aperture spacing is 1 cm.

4. The interplant mycorrhizal nutrient transfer and cultivation device according to claim 3, characterized in that: A white nylon mesh is pasted on one side of the perforated glass plate.

5. The interplant mycorrhizal nutrient transfer and cultivation device according to claim 4, characterized in that: The white nylon mesh is divided into a white nylon mesh with a pore size of 0.45 μm and a white nylon mesh with a pore size of 37 μm. The white nylon mesh with a pore size of 0.45 μm only allows soil solution to pass through, but does not allow hyphae and root systems to pass through, so as to study the net nutrient transfer between plants produced through the soil pathway; the white nylon mesh with a pore size of 37 μm only allows hyphae and soil solution to pass through, but does not allow root systems to pass through, so as to study the net nutrient transfer between plants produced through the mycorrhiza and soil pathways.

6. A method for cultivating a plant mycorrhizal nutrient transfer device according to any one of claims 1 to 5, characterized in that: The following steps are involved: Soil pathways for nutrient transfer between plants are used by: S101, selecting the organic glass plate (2) as a porous glass plate, and pasting a white nylon mesh with a pore size of 0.45 μm on the porous glass plate, and then respectively loading the first culture chamber (11) and the second culture chamber (12) with the same volume of a mixed matrix of forest topsoil and perlite containing ectomycorrhizal fungi, and transplanting one plant into each of the first culture chamber (11) and the second culture chamber (12), the plant on the side pasted with the white nylon mesh with a pore size of 0.45 μm is labeled with carbon and nitrogen isotopes, and is referred to as the donor plant, and the other plant is not labeled with carbon and nitrogen isotopes, and is referred to as the recipient plant; S102. After 2-3 months of transplanting, after successful inoculation of ectomycorrhizal fungi, the donor plants are labeled with carbon and nitrogen isotopes using 99.7atom% urea and 99atom% 13 CO2; S103. Before labeling and 6-12 months after labeling, investigate the ectomycorrhizal infection rate, aboveground and underground biomass, total C% and atom% of donor and recipient plants. 13 C, full N% and atom% 15 N, calculates the carbon transfer rate and nitrogen transfer rate between plants through soil pathways; Soil pathways for nutrient transfer between plants are used by: S201, selecting the organic glass plate (2) as a porous glass plate, and pasting a white nylon mesh with a pore size of 37 μm on the porous glass plate, and then respectively loading the first culture chamber (11) and the second culture chamber (12) with the same volume of a mixed matrix of forest topsoil and perlite containing ectomycorrhizal fungi, and transplanting one plant into each of the first culture chamber (11) and the second culture chamber (12), the plant on the side pasted with the white nylon mesh with a pore size of 37 μm is labeled with carbon and nitrogen isotopes, and is referred to as the donor plant, and the other plant is not labeled with carbon and nitrogen isotopes, and is referred to as the recipient plant; S202. After 2-3 months of transplanting, after successful inoculation of ectomycorrhizal fungi, the donor plants are labeled with carbon and nitrogen isotopes using 99.7atom% urea and 99atom% 13 CO2; S203. Before labeling and 6-12 months after labeling, investigate the ectomycorrhizal infection rate, aboveground and underground biomass, total C% and atom% of donor and recipient plants. 13 C. Full N% and atom% 15 N, calculate the carbon transfer rate and nitrogen transfer rate between plants produced by mycorrhiza and root pathways; The mycorrhizae, root systems and soil pathways of nutrient transfer between plants are used to: S301, there is no organic glass plate (2) in the middle of the culture box (1), so that hyphae, roots and soil ions are allowed to pass through; a plant is transplanted on each side of the culture box (1), one of which is labeled with carbon and nitrogen isotopes and serves as a donor plant, and the other plant is not labeled with carbon and nitrogen isotopes and serves as a recipient plant; S302. 2-3 months after transplanting, after successful inoculation of ectomycorrhizal fungi, the donor plants are labeled with carbon and nitrogen isotopes using 99.7atom% urea and 99atom% 13 CO2; S303. Before and 6-12 months after labeling, investigate the ectomycorrhizal infection rate, aboveground and underground biomass, total C% and atom% of donor and recipient plants. 13 C. Full N% and atom% 15 N, calculate the carbon transfer rate and nitrogen transfer rate between plants produced by mycorrhizal and root pathways.

7. The method of a plant mycorrhizal nutrient transfer cultivation device according to claim 6, characterized in that: The depth of the forest topsoil with ectomycorrhizal fungi is 10-20 cm, and the volume ratio of the forest topsoil with ectomycorrhizal fungi to perlite in the mixed matrix is ​​2:1.

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