Bleaching method for specimen with hydroxyheme deposition caused by formaldehyde oxidation

Abstract

The invention belongs to the technical field of biological plasticization, and provides a bleaching method for a specimen with hydroxyheme deposition caused by formaldehyde oxidation. The method comprises the following steps: soaking a specimen in a potassium permanganate solution for reaction to obtain a specimen with dark brown deep muscles; soaking the obtained specimen with dark brown deep muscles in an oxalic acid solution for reaction to obtain a specimen with reddish deep muscles; the obtained specimen with the deep muscle being reddish is soaked in hydrogen peroxide for a reaction, the deep muscle of the specimen is close to white, and bleaching of the specimen is completed; the specimen is a specimen of hydroxyheme deposition caused by formaldehyde oxidation. According to the method, the dark red color of the hydroxyheme deposition specimen caused by formaldehyde oxidation can be effectively removed, the specimen color is closer to white, meanwhile, the integrity of the specimen can be guaranteed, and teaching, exhibition and biological plasticization are better facilitated.

Description

Technical Field

[0001] The invention relates to the technical field of biological plastination, and in particular to a bleaching method for a specimen of hydroxyhemoglobin deposition caused by formaldehyde oxidation. Background Art

[0002] Most human specimens are preserved with formalin fixative. Although the specimens can be preserved for a long time, as the preservation time increases, the specimens will be oxidized by formaldehyde. This oxidation phenomenon will cause the deposition of hydroxyhemoglobin, making the specimens appear dark red or darker oxidized color. This requires bleaching of human specimens.

[0003] The most commonly used method for specimen bleaching is to use a certain concentration of hydrogen peroxide to treat human specimens. However, this conventional treatment method has no obvious effect on specimens with hydroxyhemoglobin deposition caused by formaldehyde oxidation. Moreover, using this conventional treatment method to bleach specimens for a long time will cause the muscle structure to loosen, and the cancellous bone and compact bone to break, thereby affecting the overall appearance of the human specimen and even causing the human specimen to be scrapped.

[0004] Therefore, there is an urgent need to provide a method for effectively bleaching specimens with hydroxyhemoglobin deposition caused by formaldehyde oxidation. Summary of the invention

[0005] In view of this, the present invention provides a method for bleaching specimens with hydroxyhemoglobin deposition caused by formaldehyde oxidation, so as to solve the problem that the existing bleaching methods cannot effectively bleach specimens with hydroxyhemoglobin deposition caused by formaldehyde oxidation.

[0006] In order to achieve the above object, the present invention adopts the following technical solution:

[0007] The present invention provides a bleaching method for a specimen in which hydroxyhemoglobin is deposited due to formaldehyde oxidation, comprising the following steps:

[0008] 1) Immerse the specimen in potassium permanganate solution to react and obtain a dark brown specimen of deep muscle;

[0009] 2) immersing the dark brown deep muscle specimen obtained in step 1) in an oxalic acid solution for reaction to obtain a slightly red deep muscle specimen;

[0010] 3) immersing the slightly red deep muscle specimen obtained in step 2) in hydrogen peroxide to react, so that the deep muscle of the specimen turns to its original color, and the bleaching of the specimen is completed;

[0011] The specimen is a specimen of hydroxyhemoglobin deposition caused by formaldehyde oxidation.

[0012] Preferably, the mass concentration of the potassium permanganate solution in step 1) is 0.5% to 1%; and the reaction time is 8 to 12 hours.

[0013] Preferably, the mass concentration of the oxalic acid solution in step 2) is 1% to 2%; and the reaction time is 8 to 12 hours.

[0014] Preferably, the mass concentration of the hydrogen peroxide in step 3) is 6% to 8%; and the reaction time is 12 to 24 hours.

[0015] Preferably, before the reaction in step 1), step 2) and step 3), an independent washing step is also included, and the washing solution in the washing step independently includes water.

[0016] It can be seen from the above technical solution that compared with the prior art, the present invention has the following beneficial effects:

[0017] The present invention uses potassium permanganate, oxalic acid and hydrogen peroxide to bleach the specimens with hydroxyhemoglobin deposition caused by formaldehyde oxidation, so that the color of the specimens is close to white. The existing conventional bleaching method uses hydrogen peroxide, a strong oxidant, to bleach the specimens. When the specimens are bleached with hydrogen peroxide having a mass concentration of 5 to 10%, the color of the specimens cannot be close to white, which requires prolonging the bleaching time. However, due to the strong oxidizing property of hydrogen peroxide, long-term bleaching and soaking will cause the muscle structure of the specimens to loosen, the spongy bone and the compact bone to break, and the color will not meet the requirements. Even when the color of the specimens approaches white, the specimens will become loose and scrapped due to the long soaking time in the strong oxidizing hydrogen peroxide. The present invention uses potassium permanganate, oxalic acid and hydrogen peroxide to control the degree of oxidation of the specimen, so there will be no excessive oxidation of the specimen caused by too long immersion time or too strong oxidizing property. Therefore, the method of the present invention can effectively bleach the specimen within a time without causing damage, solving the problem that the existing conventional bleaching method cannot effectively bleach the specimen with hydroxyhemoglobin deposition caused by formaldehyde oxidation. At the same time, the method of the present invention can also ensure the integrity of the specimen. In terms of exhibition, the original structure of the specimen can be clearly distinguished; in terms of plasticization, the color of the specimen can be made closer to white. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0019] Figure 1The upper limb specimens in Example 1 in which hydroxyhemoglobin is deposited due to formaldehyde oxidation before and after bleaching are shown, wherein: Figure 1 Figure a shows the upper limb specimen before bleaching. Figure 1 Figure b shows the upper limb specimen after bleaching;

[0020] Figure 2 The diagram shows the orbital specimen in Example 2 before and after bleaching due to the deposition of hydroxyhemoglobin by formaldehyde oxidation, wherein: Figure 2 Figure a shows the eye socket specimen before bleaching. Figure 2 b is a display of the orbital specimen after bleaching;

[0021] Figure 3 The diagram is a display of the internal organ specimens in Example 3 in which hydroxyhemoglobin is deposited due to formaldehyde oxidation before and after bleaching, wherein: Figure 3 Figure a shows the internal organ specimens before bleaching. Figure 3 Figure b is a display of the internal organ specimen after bleaching. DETAILED DESCRIPTION

[0022] The present invention provides a bleaching method for a specimen in which hydroxyhemoglobin is deposited due to formaldehyde oxidation, comprising the following steps:

[0023] 1) Immerse the specimen in potassium permanganate solution to react and obtain a dark brown specimen of deep muscle;

[0024] 2) immersing the dark brown deep muscle specimen obtained in step 1) in an oxalic acid solution for reaction to obtain a slightly red deep muscle specimen;

[0025] 3) immersing the slightly red deep muscle specimen obtained in step 2) in hydrogen peroxide to react, so that the deep muscle of the specimen turns to its original color, and the bleaching of the specimen is completed;

[0026] The specimen is a specimen of hydroxyhemoglobin deposition caused by formaldehyde oxidation.

[0027] In the present invention, the mass concentration of the potassium permanganate solution in step 1) is 0.5% to 1%, preferably 0.55% to 0.9%, more preferably 0.6% to 0.85%, more preferably 0.65% to 0.7%; the reaction time is 8 to 12 hours, preferably 8.5 to 11 hours, more preferably 9 to 10.5 hours, more preferably 10 hours.

[0028] In the present invention, step 1) utilizes the strong oxidizing property of potassium permanganate solution to react with the product of formaldehyde oxidation, so that the deep muscle of the hydroxyhemoglobin deposition specimen turns into dark brown.

[0029] In the present invention, the mass concentration of the oxalic acid solution in step 2) is 1% to 2%, preferably 1.2% to 1.8%, more preferably 1.3% to 1.6%, and more preferably 1.5%; the reaction time is 8 to 12 hours, preferably 8.5 to 11 hours, more preferably 9 to 10.5 hours, and more preferably 10 hours.

[0030] In the present invention, step 2) utilizes the oxidizing property of oxalic acid solution to decolorize potassium permanganate, thereby causing the deep muscle of the hydroxyhemoglobin deposition specimen to turn reddish.

[0031] In the present invention, the mass concentration of the hydrogen peroxide in step 3) is 6% to 8%, preferably 6.2% to 7.8%, more preferably 6.5% to 7.5%, and more preferably 7%; the reaction time is 12 to 24 hours, preferably 13 to 22 hours, more preferably 15 to 20 hours, and more preferably 18 hours.

[0032] In the present invention, step 3) utilizes the strong oxidizing property of hydrogen peroxide to further fade the hydroxyhemoglobin deposition specimen, thereby changing the deep muscle of the hydroxyhemoglobin deposition specimen to its original color.

[0033] In the present invention, the primary color in step 3) is close to white.

[0034] In the present invention, before the reaction in step 1), step 2) and step 3), an independent washing step is also included, and the washing solution of the washing step independently includes water.

[0035] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0036] Example 1

[0037] 1) Put the upper limb specimens with hydroxyhemoglobin deposited due to formaldehyde oxidation into running water, rinse off the residual formaldehyde solution, and then immerse in a potassium permanganate solution with a mass concentration of 0.5% for 12 hours to obtain a specimen with a dark brown deep muscle;

[0038] 2) placing the dark brown deep muscle specimen obtained in step 1) into running water, washing away the residual potassium permanganate solution, and then immersing in a 1% oxalic acid solution for 8 hours to obtain a slightly red deep muscle specimen;

[0039] 3) The slightly red deep muscle specimen obtained in step 2) is placed in running water, and after the residual oxalic acid solution is rinsed off, it is immersed in 6% hydrogen peroxide and reacted for 24 hours. When the deep muscle of the specimen is nearly white, it is removed and rinsed with running water to complete the bleaching of the specimen.

[0040] The upper limb specimens in this example, which were oxidized by formaldehyde and resulted in the deposition of hydroxyhemoglobin, are shown in the following figure before and after bleaching. Figure 1 As shown, Figure 1 a in the figure is the upper limb specimen before bleaching. Figure 1 b is the upper limb specimen after bleaching. Figure 1 From a and b in FIG. 1 , it can be clearly seen that the specimen bleached by the method of the present invention can remove the dark red color caused by oxidation and make the specimen close to white.

[0041] Example 2

[0042] 1) Place the orbital specimens with hydroxyhemoglobin deposited due to formaldehyde oxidation in running water, rinse off the residual formaldehyde solution, and then immerse in a 0.8% potassium permanganate solution for 8 hours to obtain a specimen with dark brown deep muscle;

[0043] 2) placing the dark brown deep muscle specimen obtained in step 1) into running water, washing away the residual potassium permanganate solution, and then immersing in a 1.5% oxalic acid solution for 8 hours to obtain a slightly red deep muscle specimen;

[0044] 3) The slightly red deep muscle specimen obtained in step 2) is placed in running water, and after the residual oxalic acid solution is rinsed off, it is immersed in 7% hydrogen peroxide and reacted for 12 hours. The deep muscle of the specimen is nearly white, and it is removed and rinsed with running water to complete the bleaching of the specimen.

[0045] The orbital specimens in this example, in which hydroxyhemoglobin is deposited due to formaldehyde oxidation, are shown in the following figure before and after bleaching. Figure 2 As shown, Figure 2 a in the figure is the orbital specimen before bleaching. Figure 2 The b in the figure is the orbital specimen after bleaching. Figure 2 From a and b in the figure, it can be clearly seen that the specimen bleached by the method of the present invention can effectively remove the dark red color caused by oxidation, making the specimen color close to white, and can ensure the integrity of the specimen.

[0046] Example 3

[0047] 1) placing the internal organ specimens in which hydroxyhemoglobin is deposited due to formaldehyde oxidation into running water, washing away the residual formaldehyde solution, and then immersing them in a potassium permanganate solution with a mass concentration of 1%, reacting for 8 hours, and obtaining specimens with deep muscle being dark brown, wherein the internal organs include kidneys, liver, heart and male urinary system;

[0048] 2) placing the dark brown deep muscle specimen obtained in step 1) into running water, washing away the residual potassium permanganate solution, and then immersing in a 2% oxalic acid solution for 8 hours to obtain a slightly red deep muscle specimen;

[0049] 3) The slightly red deep muscle specimen obtained in step 2) is placed in running water, and after the residual oxalic acid solution is rinsed off, it is immersed in 8% hydrogen peroxide and reacted for 12 hours. When the deep muscle of the specimen is nearly white, it is removed and rinsed with running water to complete the bleaching of the specimen.

[0050] The internal organ specimens in this example in which formaldehyde oxidation leads to hydroxyhemoglobin deposition are shown in the following figure before and after bleaching. Figure 3 As shown, Figure 3 a in the figure is the internal organ specimen before bleaching. Figure 3 b in the figure is the internal organ specimen after bleaching. Figure 3 It can be clearly seen that the color of the specimen after bleaching by the method of the present invention is close to white, while the integrity of the specimen can be ensured, and the original structure of the specimen can be clearly distinguished, which is more conducive to teaching, exhibition, and biological plastination.

[0051] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for bleaching a specimen in which hydroxyhemoglobin is deposited due to formaldehyde oxidation, characterized in that: The steps include: 1) Immerse the specimen in potassium permanganate solution to react and obtain a dark brown specimen of deep muscle; 2) immersing the dark brown deep muscle specimen obtained in step 1) in an oxalic acid solution for reaction to obtain a slightly red deep muscle specimen; 3) immersing the slightly red deep muscle specimen obtained in step 2) in hydrogen peroxide to react, so that the deep muscle of the specimen turns to its original color, and the bleaching of the specimen is completed; The specimen is a specimen of hydroxyhemoglobin deposition caused by formaldehyde oxidation.

2. The method for bleaching a specimen of hydroxyhemoglobin deposition caused by formaldehyde oxidation according to claim 1, characterized in that: The mass concentration of the potassium permanganate solution in step 1) is 0.5% to 1%; The reaction time is 8 to 12 hours.

3. The method for bleaching a specimen of hydroxyhemoglobin deposition caused by formaldehyde oxidation according to claim 2, characterized in that: The mass concentration of the oxalic acid solution in step 2) is 1% to 2%; The reaction time is 8 to 12 hours.

4. A method for bleaching a specimen in which hydroxyhemoglobin is deposited due to formaldehyde oxidation according to any one of claims 1 to 3, characterized in that: The mass concentration of the hydrogen peroxide in step 3) is 6% to 8%; The reaction time is 12 to 24 hours.

5. The method for bleaching a specimen of hydroxyhemoglobin deposition caused by formaldehyde oxidation according to claim 4, characterized in that: Before the reaction in step 1), step 2) and step 3), an independent washing step is also included, and the washing solution in the washing step independently includes water.

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