Acaricidal composition and application thereof

Abstract

The invention belongs to the field of pesticide acaricides, and relates to an acaricidal composition and application thereof. The active components of the acaricidal composition comprise an active component A and an active component B, the active component A is a compound represented by a formula (I), the active component B is any one of bistrifluron and trifluoro-sassen, and the mass ratio of the active component A to the active component B is 1: 30-24: 1. The acaricidal composition disclosed by the invention has the advantages of synergism, dosage reduction and the like, and can be used for preventing and treating agricultural and forestry pest mites and sanitary pest mites.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide acaricides, and in particular relates to an acaricide composition and its application in agricultural and forestry harmful mites and sanitary harmful mites. Background Art

[0002] Fluometra thioether, its chemical name: (4'-chloro-6-fluoro-4-methyl-[1,1'-biphenyl]-3-yl)(2,2,2-trifluoroethyl) thioether, has the following chemical structure:

[0003]

[0004] In the process of agricultural production, the control of various types of pest mites mainly relies on the application of a large number of pesticide acaricide products. The large-scale, frequent and unreasonable use of pesticide acaricides has led to the continuous enhancement of the resistance of pest mites, which has caused more serious damage to crops and made prevention and control more difficult. By mixing two or more agents with different mechanisms of action, it is possible to simultaneously control different pests, improve the efficacy of the agents, and reduce the generation of pest resistance. The compound of formula (I) and bistrifluan or trifluanid has a significant synergistic effect on the control of herbivorous spider mites, and the compound acaricide composition of the compound of formula (I) and bistrifluan or trifluanid and its application have not been reported so far. Summary of the invention

[0005] Based on the above situation, the purpose of the present invention is to provide a miticide composition, which is mainly used for controlling herbivorous mites. The miticide composition or its preparation can enhance the efficacy, reduce the dosage, and at the same time prolong the effective period and delay the development of drug resistance.

[0006] To achieve the above object, the technical solution adopted by the present invention is: an acaricidal composition, the active ingredients of the acaricidal composition include active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I):

[0007] Active ingredient B is any one of triflumuron or trifluanid;

[0008] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 24:1;

[0009] Furthermore, the mass ratio of the compound of formula (I) to bistrifluan is 1:25 to 24:1;

[0010] Furthermore, the mass ratio of the compound of formula (I) to bistrifluan is 1:25, 1:20, 1:15, 1:10, 1:6, 1:3, 6:1, 8:1, 16:1, 20:1, 24:1;

[0011] Furthermore, the mass ratio of the compound of formula (I) to bistrifluan is 1:20 to 16:1;

[0012] Furthermore, the mass ratio of the compound of formula (I) to bistrifluan is 1:20, 1:15, 1:10, 1:6, 1:3, 6:1, 8:1, 16:1;

[0013] Furthermore, the mass ratio of the compound of formula (I) to trifluoxetine is 1:26 to 21:1;

[0014] Furthermore, the mass ratio of the compound of formula (I) to trifluoxetine is 1:26, 1:24, 1:10, 1:5, 1:4, 5:1, 10:1, 14:1, 18:1, 21:1;

[0015] Furthermore, the mass ratio of the compound of formula (I) to trifluoxetine is 1:26 to 14:1;

[0016] Furthermore, the mass ratio of the compound of formula (I) to trifluoxetine is 1:26, 1:24, 1:10, 1:5, 1:4, 5:1, 10:1, 14:1;

[0017] Furthermore, based on the total weight of the acaricidal composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the acaricidal composition is 1 to 80 wt%;

[0018] Furthermore, based on the total mass of the acaricidal composition being 100 wt%, the sum of the contents of the compound of formula (I) and bistrifluan in the acaricidal composition is 2 to 50 wt%;

[0019] Furthermore, based on the total mass of the acaricidal composition being 100wt%, the sum of the contents of the compound of formula (I) and bistrifluan in the acaricidal composition is 5wt%, 10wt%, 15wt%, 20wt%, 24wt%, 42wt%, 45wt%, 50wt%;

[0020] Furthermore, based on the total mass of the acaricidal composition being 100 wt%, the sum of the contents of the compound of formula (I) and trifluoxetine in the acaricidal composition is 2 to 60 wt%;

[0021] Furthermore, based on the total mass of the acaricidal composition as 100wt%, the sum of the contents of the compound of formula (I) and trifluoxetine in the acaricidal composition is 6.5wt%, 8wt%, 12wt%, 15wt%, 24wt%, 28wt%, 36wt%, 40wt%, 45wt%, 50wt%;

[0022] Furthermore, the acaricide composition includes agriculturally acceptable auxiliary ingredients in addition to the active ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers;

[0023] Further, the wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, alpha olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, ethylene glycol polyoxyethylene polyoxypropylene ether, fatty alcohol ethoxylate, isomeric alcohol polyoxyethylene ether, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, saponin powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0024] Further, the dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, block polyether phosphate, arylphenol polyoxyethylene ether sulfate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acid, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0025] Further, the emulsifier is selected from one or more of calcium dodecylbenzene sulfonate, dodecylbenzene sulfonate amine, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, benzylphenol polyoxyethylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol polyoxyethylene ether, alkylphenol ether phosphate and styrylphenol polyoxyethylene ether phosphate; and / or

[0026] Further, the thickener is selected from one or more of xanthan gum, organic bentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0027] Further, the disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0028] Further, the antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0029] Furthermore, the defoaming agent is selected from C 10 -C 20Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0030] Further, the solvent is selected from one or more of benzene, toluene, xylene, trimethylbenzene, durene, methanol, ethanol, isopropanol, n-butanol, isooctyl alcohol, dimethyl sulfoxide, N,N-dimethylformamide, N,N-dimethylacetamide, N,N-dimethyldecylamide, N-methylpyrrolidone, cyclohexanone, propylene carbonate, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0031] Further, the preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, propyl p-hydroxybenzoate, butyl p-hydroxybenzoate, isothiazolinones, benzimidazoles, iodopropargyls, pyridinethiones, kasone and 1,2-benzisothiazolin-3-one; and / or

[0032] Further, the stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0033] Further, the synergist is selected from synergistic phosphorus, synergistic ether, synergistic amine; and / or

[0034] Further, the carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives.

[0035] Furthermore, the acaricide composition can be prepared into a formulation form permitted in agriculture, and the formulation form is selected from a solid formulation and / or a liquid formulation;

[0036] Furthermore, the solid preparation includes powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0037] Further, the liquid preparation includes a soluble solution, a soluble gel, an oil, a film-spreading oil, an emulsifiable concentrate, a latex, a dispersible liquid, an ointment, an aqueous emulsion, an oil emulsion, a microemulsion, a fat, a suspension, a microcapsule suspension, an oil suspension, a dispersible oil suspension, a suspoemulsion, a microcapsule suspension-suspension, a microcapsule suspension-water emulsion or a microcapsule suspension-suspoemulsion;

[0038] Furthermore, the solid preparation is selected from wettable powders and water dispersible granules; the liquid preparation is selected from soluble solutions, emulsifiable concentrates, aqueous emulsions, suspensions, suspoemulsions, microemulsions, and dispersible oil suspensions;

[0039] Furthermore, the solid preparation is selected from wettable powders and water dispersible granules; the liquid preparation is selected from emulsifiable concentrates, aqueous emulsions, suspensions, suspoemulsions, and microemulsions;

[0040] Furthermore, when the acaricide composition is a wettable powder, the mass proportions of the components are: 1-40 parts of active ingredient A, 1-40 parts of active ingredient B, 3-10 parts of dispersant, 1-5 parts of wetting agent, and the balance is made up of fillers; the preparation method of the wettable powder is to pre-crush the active ingredient and other auxiliary agents and mix them evenly, and then grind them by a jet mill to a fineness that meets the requirement that at least 98wt% passes through a 45μm test sieve to obtain a wettable powder product.

[0041] Furthermore, when the acaricide composition is a water-dispersible granule, the mass proportions of the components are: 1-40 parts of active ingredient A, 1-40 parts of active ingredient B, 3-20 parts of dispersant, 1-10 parts of wetting agent, 1-5 parts of disintegrant, and the balance is made up of fillers; the preparation method of the water-dispersible granules is to add the active ingredient to the carrier according to the formula ratio, and add surfactants and other functional additives thereto, mix, add 10-25% of water after air flow pulverization, and then knead, granulate, dry, and sieve to obtain a water-dispersible granule product.

[0042] Furthermore, when the acaricide composition is an emulsifiable concentrate preparation, the mass proportions of the components are: 1-40 parts of active ingredient A, 1-40 parts of active ingredient B, 5-30 parts of emulsifier, 0-5 parts of synergist, and the solvent makes up the balance; the emulsifiable concentrate preparation method is to add the active ingredients into the solvent according to the formula ratio of the embodiment, and add surfactants and other functional additives thereto, and stir and mix them evenly in a stirring mixing kettle to obtain an emulsifiable concentrate product.

[0043] Furthermore, when the acaricide composition is an aqueous emulsion, the mass fractions of the components are: 1-40 parts of active ingredient A, 1-40 parts of active ingredient B, 3-30 parts of emulsifier, 5-50 parts of solvent, 2-15 parts of stabilizer, 1-5 parts of antifreeze, 0.1-8 parts of defoamer, 0.2-2 parts of thickener, and deionized water makes up the balance; the preparation method of the aqueous emulsion is to dissolve the active ingredient in the solvent according to the formula ratio of the embodiment, add the emulsifier to dissolve it into a uniform oil phase, mix the deionized water and the antifreeze together to form a uniform water phase; add the water phase to the oil phase, stir evenly, add the defoamer, and form a well-dispersed aqueous emulsion product through high-speed shearing.

[0044] Furthermore, when the acaricide composition is a suspension, the mass fractions of the components are: 1-40 parts of active ingredient A, 1-40 parts of active ingredient B, 1-15 parts of wetting and dispersing agent, 1-5 parts of antifreeze agent, 0.1-2 parts of thickener, 0.1-0.8 parts of defoaming agent, 0-10 parts of solvent, 0-5 parts of stabilizer, and deionized water makes up the balance; the preparation method of the suspension is to place the active ingredient, surfactant and other functional additives in a reaction kettle in sequence according to the formula ratio, add water to mix evenly, and obtain the suspension product by high-speed shearing, wet sand grinding, and finally filtering for homogeneity.

[0045] Furthermore, when the acaricide composition is a suspoemulsion, the mass fractions of the components are: 1-40 parts of active ingredient A, 1-40 parts of active ingredient B, 1-10 parts of emulsifier, 1-10 parts of dispersant, 1-5 parts of antifreeze, 0.1-2 parts of thickener, 0.1-0.8 parts of defoamer, 1-40 parts of solvent, 0-5 parts of stabilizer, and deionized water makes up the balance; the preparation method of the suspoemulsion is to completely dissolve the active ingredient A with the solvent according to the formula ratio, add the emulsifier to form the oil phase; wet sand grind the active ingredient B with the wetting dispersant, antifreeze, defoamer, deionized water, etc. to D 90 The suspension mother liquid is prepared by mixing the oil phase into the suspension mother liquid and stirring evenly, and then adding the thickener and stirring and shearing evenly to prepare the suspoemulsion preparation of the composition of the present invention.

[0046] Furthermore, when the acaricide composition is a microemulsion, the mass fractions of the components are: 1-40 parts of active ingredient A, 1-40 parts of active ingredient B, 8-30 parts of emulsifier, 5-15 parts of solvent, 2-15 parts of stabilizer, 1-5 parts of antifreeze, 0.1-8 parts of defoamer, and deionized water makes up the balance; the preparation method of the microemulsion is to dissolve the active ingredient in the solvent according to the formula ratio of the embodiment, add the emulsifier to dissolve it into a uniform oil phase, mix the deionized water and antifreeze together to form a uniform water phase; add the water phase to the oil phase, or add the oil phase to the water phase, and stir evenly to form a microemulsion product.

[0047] The invention also discloses the use of the above-mentioned acaricidal composition for controlling harmful mites;

[0048] Furthermore, the harmful mites are herbivorous harmful mites;

[0049] Furthermore, the phytophagous pest mites are Panonychus citri McGregor, Eotetranychus kankitus (Ehara), Phyllocoptes oleivora, Tetranychus cinnabarinus (Boisduval), and Tetranychus urticae Koch.

[0050] Furthermore, the acaricidal composition and / or its preparation is applied at an effective dose to the harmful mites to be controlled or to the medium where they grow.

[0051] The acaricidal composition of the present invention has the following advantages:

[0052] 1) The acaricidal composition of the present invention exhibits a synergistic effect within a certain ratio range and can effectively control a variety of vegetative pest mites;

[0053] 2) The two active ingredients in the acaricidal composition of the present invention have different action mechanisms, which effectively delay the development of drug resistance in harmful mites and extend the service life of the product;

[0054] 3) The acaricide composition of the present invention is environmentally friendly, safe and efficient, and its popularization and application has huge economic and social benefits. DETAILED DESCRIPTION

[0055] The present invention is further described below with reference to the examples. The percentages in the examples are all percentages by weight, but the present invention is not limited thereto.

[0056] The composition of the present invention can be provided in the form of a formulation. It can be prepared into a wettable powder, a water dispersible granule, an emulsifiable concentrate, an aqueous emulsion, a suspension, a suspoemulsion, a microemulsion, etc. as required. The content of the active ingredient in the composition of the present invention depends on the application amount when used alone, and also depends on the mixing ratio and the degree of synergistic effect. The optimal range of the active ingredient content varies according to the formulation type of the composition.

[0057] Preparation Example:

[0058] Preparation Example 1

[0059] 5% compound of formula (Ⅰ)·bistrifluan emulsifiable concentrate (4+1)

[0060] Formula composition: 4% of the compound of formula (I), 1% of bistrifluan, 15% of propylene carbonate, 3% of calcium dodecylbenzenesulfonate, 15% of fatty alcohol polyoxyethylene ether, 1% of N,N-dimethylformamide, 3% of corn oil, 30% of cyclohexanone, and the balance is made up of methyl oleate;

[0061] Preparation method: firstly add the active ingredient into the solvent and completely dissolve it, then add the emulsifier and the like and stir evenly to form a uniform and transparent oily liquid, and then fill it to prepare the emulsifiable concentrate preparation of the composition of the present invention.

[0062] Preparation Example 2

[0063] 6.5% Formula (Ⅰ) compound·Trifluanidazole EC (5.5+1)

[0064] Formula composition: 5.5% of the compound of formula (I), 1% of triflumuron, 10% of castor oil polyoxyethylene ether, 3.5% of calcium dodecylbenzenesulfonate, 10.5% of fatty alcohol polyoxyethylene ether, 6% of corn oil, 32% of cyclohexanone, and the balance is made up of methyl oleate;

[0065] Preparation method: firstly add the active ingredient into the solvent and completely dissolve it, then add the emulsifier and the like and stir evenly to form a uniform and transparent oily liquid, and then fill it to prepare the emulsifiable concentrate preparation of the composition of the present invention.

[0066] Preparation Example 3

[0067] 24% compound of formula (I)·bistrifluanone suspension concentrate (21+3)

[0068] Formula composition: 21% of compound of formula (Ⅰ), 3% of bistrifluan, 2% of alkylnaphthalene sulfonate formaldehyde condensate, 2% of sodium lignin sulfonate, 5% of polycarboxylate, 3% of EO-PO block copolymer, 0.5% of naphthalene sulfonate, 0.5% of organosilicon defoamer, 0.2% of xanthan gum, 1% of magnesium aluminum silicate, 4% of ethylene glycol, 0.5% of sodium benzoate, and deionized water is added to 100%;

[0069] Preparation method: add a wetting dispersant and a defoamer to the measured deionized water and stir evenly, then add the active ingredient and stir evenly, use zirconium beads and a sand mill to wet grind until D90 (90% of the particle size) is less than 5μm to obtain a crushed slurry. Add a thickener, an antifreeze agent, and a preservative to the crushed slurry and mix evenly, add deionized water to 100%, and high-speed shear to obtain a suspension.

[0070] Preparation Example 4

[0071] 36% Formula (Ⅰ) compound·Trifluoxetine suspension (30+6)

[0072] Formula composition: 30% of the compound of formula (I), 6% of triflumuron, 3.5% of polycarboxylate, 3% of EO-PO block copolymer, 1.5% of sodium dodecyl sulfate, 0.8% of naphthalenesulfonate, 0.5% of organic silicon defoamer, 0.2% of xanthan gum, 1% of magnesium aluminum silicate, 4% of ethylene glycol, 1% of glycerol, 0.5% of sodium benzoate, and deionized water is added to 100%;

[0073] Preparation method: add a wetting dispersant and a defoamer to the measured deionized water and stir evenly, then add the active ingredient and stir evenly, use zirconium beads and a sand mill to wet grind until D90 (90% of the particle size) is less than 5μm to obtain a crushed slurry. Add a thickener, an antifreeze agent, and a preservative to the crushed slurry and mix evenly, add deionized water to 100%, and high-speed shear to obtain a suspension.

[0074] Preparation Example 5

[0075] 45% compound of formula (I)·bistrifluan water dispersible granules (35+10)

[0076] Formula composition: 35% of the compound of formula (I), 10% of bistrifluan, 8% of lignin sulfonate, 2% of α-olefin sulfonate, 8% of alkylnaphthalene sulfonate formaldehyde condensate, 1% of ammonium chloride, 1% of sodium carbonate, 10% of attapulgite, and bentonite added to 100%;

[0077] Preparation method: first mix evenly, then grind to less than 15μm by air flow mill, mix evenly with water, granulate by rotary granulator, dry at 60℃ to a moisture content of ≤3%, select 20-mesh and 60-mesh test sieves for sieving, discard the large particles on the top and the dust filtered at the bottom, and prepare water-dispersible granules.

[0078] Preparation Example 6

[0079] 40% compound of formula (I)·trifluanidazole water dispersible granules (32+8)

[0080] Formula composition: 32% of the compound of formula (I), 8% of trifloxystrobin, 8% of lignin sulfonate, 2% of α-olefin sulfonate, 8% of alkylnaphthalene sulfonate formaldehyde condensate, 1.5% of ammonium chloride, 1% of sodium carbonate, 12% of kaolin, and bentonite added to 100%;

[0081] Preparation method: first mix evenly, then grind to less than 15μm by air flow mill, mix evenly with water, granulate by rotary granulator, dry at 60℃ to a moisture content of ≤3%, select 20-mesh and 60-mesh test sieves for sieving, discard the large particles on the top and the dust filtered at the bottom, and prepare water-dispersible granules.

[0082] Preparation Example 7

[0083] 42% compound of formula (Ⅰ)·bistrifluanyl wettable powder (36+6)

[0084] Formula composition: 36% of the compound of formula (I), 6% of bistrifluan, 4.5% of naphthalene sulfonate, 0.8% of alkylphenol polyoxyethylene ether methyl ether condensate sulfate, 15% of lignin sulfonate sodium salt, 3% of sodium dodecyl sulfate, 8% of white carbon black, and kaolin added to 100%;

[0085] Preparation method: pre-crush and mix evenly, then grind by air flow mill to a fineness that satisfies the requirement that at least 98wt% passes through a 45μm test sieve to obtain a wettable powder.

[0086] Preparation Example 8

[0087] 45% formula (Ⅰ) compound·trifluanidazole wettable powder (30+15)

[0088] Formula composition: 30% of the compound of formula (I), 15% of triflumuron, 5% of naphthalene sulfonate, 1% of alkylphenol polyoxyethylene ether methyl ether condensate sulfate, 16% of lignin sulfonate sodium salt, 2.5% of sodium dodecyl sulfate, 10% of bentonite, and kaolin added to 100%;

[0089] Preparation method: pre-crush and mix evenly, then grind by air flow mill to a fineness that satisfies the requirement that at least 98wt% passes through a 45μm test sieve to obtain a wettable powder.

[0090] Indoor biological activity assay

[0091] Indoor activity test 1

[0092] Test insect source: Panonychus citri;

[0093] Test agents: 95% triflumuron technical, 96% bistriflumuron technical, 96% compound of formula (I);

[0094] Preparation of test materials: Select adult mites raised indoors and in the same physiological state. Cut double-sided tape into 2 cm long pieces and stick them on one end of the slide. Then select healthy adult mites and stick their backs on the double-sided tape. Put 25 mites per slide into a container padded with a wet sponge, cover it with a lid, and place it at (25±1)℃. After 2 hours, examine under a microscope, remove dead and injured individuals, and make up 20 mites per slide.

[0095] Preparation of drugs: The original drug was prepared into a mother solution with acetone, and the mother solution was prepared into test drugs in different proportions with 0.1% Tween-80 aqueous solution.

[0096] Chemical treatment: Immerse the glass slide with adult mites in the chemical solution and shake it gently for 5 seconds, then take it out and absorb the excess chemical solution with absorbent paper. Place it on a white porcelain plate lined with a wet sponge, cover it with a light-transmitting plastic film, and place it at (25±1)℃ for observation.

[0097] Experimental repetition: Each treatment was repeated 4 times, and a treatment without drug was set as a blank control.

[0098] Test investigation: 48 hours after treatment, check the death of test insects, record the total number of mites and the number of dead mites, and calculate the mortality rate:

[0099]

[0100] Where:

[0101] P——mortality rate, in percentage (%);

[0102] K——indicates the number of dead mites, the unit is head;

[0103] N——represents the total number of mites treated, in heads.

[0104]

[0105] Where:

[0106] P1——adjusted mortality rate, in percentage (%);

[0107] P t ——Treatment mortality rate, expressed as percentage (%);

[0108] P0——blank control mortality rate, in percentage (%).

[0109] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the above formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0110] The LC of each drug was calculated by linear regression analysis between the probability value of mortality and the logarithmic value of the series concentration (DPS data processing system). 50 The Sun Yunpei method was used to calculate the co-toxicity coefficient (CTC) of the mixture to evaluate the activity of the test agent against the test insects; the specific calculation formula is as follows:

[0111]

[0112] Where:

[0113] ATI——Actual Toxicity Index of Mixture;

[0114] S——LC of standard acaricide 50 , the unit is milligrams per liter (mg / L);

[0115] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0116] TTI=TI A *P A +TI B *P B

[0117] Where:

[0118] TTI – Theoretical Toxicity Index of Mixtures;

[0119] TI A ——A: toxicity index of the agent;

[0120] P A ——The percentage of agent A in the mixture, in percentage (%);

[0121] TI B ——Toxicity index of agent B;

[0122] P B ——The percentage of agent B in the mixture, in percentage (%).

[0123]

[0124] Where:

[0125] CTC – Co-toxicity coefficient;

[0126] ATI——Actual Toxicity Index of Mixture;

[0127] TTI - Theoretical Toxicity Index of Mixture.

[0128] According to the classification standard of combined effects: a co-toxicity coefficient (CTC) ≥ 120 indicates a synergistic effect, a co-toxicity coefficient (CTC) ≤ 80 indicates an antagonistic effect, and a co-toxicity coefficient (CTC) 80 < CTC < 120 indicates an additive effect.

[0129] Test results:

[0130] The combined toxicity test of the compound of formula (I) and bistrifluanuron in different ratios against Panonychus citri (adult mites) is shown in Table 1. 50 The value is 3.098mg / L, and the LC value of bistrifluan to citrus mites (adult mites) 50 The value is 11.306 mg / L.

[0131] The mass ratio of the compound of formula (I) to triflubenzuron in the range of 1:25 to 24:1 showed a synergistic effect on the prevention of citrus Panonychus mites (adult mites). When the mass ratio of the compound of formula (I) to triflubenzuron was 6:1, the co-toxicity coefficient was 205.255, which was relatively high and the synergistic effect was significant.

[0132] Table 1 Indoor activity test results of compound of formula (I) combined with bistrifluanuron against Panonychus citri (adult mites)

[0133]

[0134] The combined toxicity test of different ratios of the compound of formula (I) and trifluoxetine against Panonychus citri (adult mites) is shown in Table 2. 50 The value is 15.025mg / L.

[0135] The mass ratio of the compound of formula (I) to triflumuron showed a synergistic effect on the prevention of citrus Panonychus mites (adult mites) in the range of 1:26 to 21:1. When the mass ratio of the compound of formula (I) to triflumuron was 5:1, the co-toxicity coefficient was 217.242, which was relatively high and the synergistic effect was significant.

[0136] Table 2 Indoor activity test results of the compound of formula (I) combined with trifluproline against Panonychus citri (adult mites)

[0137]

[0138]

[0139] Indoor activity test 2

[0140] Test insect source: Two-spotted spider mite;

[0141] Test agents: 95% triflumuron technical, 96% bistriflumuron technical, 96% compound of formula (I);

[0142] Preparation of test materials: Select adult mites raised indoors and in the same physiological state. Cut double-sided tape into 2 cm long pieces and stick them on one end of the slide. Then select healthy adult mites and stick their backs on the double-sided tape. Put 25 mites per slide into a container padded with a wet sponge, cover it with a lid, and place it at (25±1)℃. After 2 hours, examine under a microscope, remove dead and injured individuals, and make up 20 mites per slide.

[0143] Preparation of drugs: The original drug was prepared into a mother solution with acetone, and the mother solution was prepared into test drugs in different proportions with 0.1% Tween-80 aqueous solution.

[0144] Chemical treatment: Immerse the glass slide with adult mites in the chemical solution and shake it gently for 5 seconds, then take it out and absorb the excess chemical solution with absorbent paper. Place it on a white porcelain plate lined with a wet sponge, cover it with a light-transmitting plastic film, and place it at (25±1)℃ for observation.

[0145] Experimental repetition: Each treatment was repeated 4 times, and a treatment without drug was set as a blank control.

[0146] Test investigation: 48 hours after treatment, check the death of test insects, record the total number of mites and the number of dead mites, and calculate the mortality rate:

[0147]

[0148] Where:

[0149] P——mortality rate, in percentage (%);

[0150] K——indicates the number of dead mites, the unit is head;

[0151] N——represents the total number of mites treated, in heads.

[0152]

[0153] Where:

[0154] P1——adjusted mortality rate, in percentage (%);

[0155] P t ——Treatment mortality rate, expressed as percentage (%);

[0156] P0——blank control mortality rate, in percentage (%).

[0157] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the above formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0158] The LC of each drug was calculated by linear regression analysis between the probability value of mortality and the logarithmic value of the series concentration (DPS data processing system). 50 The Sun Yunpei method was used to calculate the co-toxicity coefficient (CTC) of the mixture to evaluate the activity of the test agent against the test insects; the specific calculation formula is as follows:

[0159]

[0160] Where:

[0161] ATI——Actual Toxicity Index of Mixture;

[0162] S——LC of standard acaricide 50 , the unit is milligrams per liter (mg / L);

[0163] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0164] TTI=TI A *P A +TI B *P B

[0165] Where:

[0166] TTI – Theoretical Toxicity Index of Mixtures;

[0167] TI A——A: toxicity index of the agent;

[0168] P A ——The percentage of agent A in the mixture, in percentage (%);

[0169] TI B ——Toxicity index of agent B;

[0170] P B ——The percentage of agent B in the mixture, in percentage (%).

[0171]

[0172] Where:

[0173] CTC – Co-toxicity coefficient;

[0174] ATI——Actual Toxicity Index of Mixture;

[0175] TTI - Theoretical Toxicity Index of Mixture.

[0176] According to the classification standard of combined effects: a co-toxicity coefficient (CTC) ≥ 120 indicates a synergistic effect, a co-toxicity coefficient (CTC) ≤ 80 indicates an antagonistic effect, and a co-toxicity coefficient (CTC) 80 < CTC < 120 indicates an additive effect.

[0177] Test results:

[0178] The results in Table 3 show that the compound of formula (I) has an LC 50 The value is 4.626mg / L, LC value of bistrifluan to Tetranychus uron (adult mite) 50 The value is 80.649 mg / L. The mass ratio of the compound of formula (I) to bistrifluan in the range of 1:20 to 20:1 shows a synergistic effect on the prevention of Tetranychus uronicus (adult mites). When the mass ratio of the compound of formula (I) to bistrifluan is 1:3, the co-toxicity coefficient is 204.344, which is relatively high and has a significant synergistic effect.

[0179] Table 3 Indoor activity test results of the compound of formula (I) and bistrifluanuron against Tetranychus urticae (adult mites)

[0180]

[0181] The combined toxicity of the compound of formula (I) and trifluoxetine in different ratios against Tetranychus urticae (adult mites) is determined as follows: The results in Table 4 show that the LC 50The value is 20.170 mg / L. The mass ratio of the compound of formula (I) to triflumuron in the range of 1:24 to 18:1 shows a synergistic effect on the prevention of Tetranychus urticae (adult mites). When the mass ratio of the compound of formula (I) to triflumuron is 1:4, the co-toxicity coefficient is 205.542, which is relatively high and has a significant synergistic effect.

[0182] Table 4 Indoor activity test results of the compound of formula (I) combined with triflumuron against Tetranychus urticae (adult mites)

[0183]

[0184]

[0185] Field efficacy examples

[0186] Field test on the efficacy of pesticides in controlling citrus red spider mites

[0187] Test crops: Citrus (Wogan);

[0188] Test subjects: Citrus spider mite;

[0189] Experimental design: 6 treatments, 3 replications, 18 plots, 2 trees per plot, randomized block arrangement, with one row of fruit trees between each plot.

[0190] Table 5 Test agents and dosage

[0191]

[0192] The test site is the citrus orchard in Shantou Village, Liuzhou City. The growth of citrus trees in the test area is basically the same, and the occurrence of citrus red spider mites has been severe in recent years. The soil fertility of the test site is high, the cultivation and management conditions are good, and it is planted all year round.

[0193] The pesticide was applied on October 2, 2021. It was sunny on the day of application, with the highest temperature of 36°C, the lowest temperature of 27°C, and the south wind at level 1. It was sunny during the test.

[0194] Application method, time and frequency: Use a motorized sprayer (WL-ABSC model) and manually spray the whole tree evenly. According to the actual field conditions and farmers' water consumption habits, the water consumption per citrus tree is determined to be 1.8L, and the amount of application is determined to be evenly applied to the leaves with a little dripping. Apply once.

[0195] Survey method: Randomly select one point from the east, west, south, north and center of each citrus plant, mark a branch at each point, and record the number of all living citrus red spider mites on the leaves of the marked branches (starting from the top leaf, no less than 5 leaves). Mainly investigate adult mites and nymph mites (can be observed with a magnifying glass). Survey once before and 3d and 7d after the drug.

[0196] Calculation method of drug efficacy: The drug efficacy is calculated according to the following formula:

[0197]

[0198]

[0199] During the experiment, the citrus trees in each experimental plot grew well and no phytotoxicity was observed in any treatment.

[0200] The results of the field trials showed that:

[0201] As can be seen from Table 6, the mixture of the compound of formula (I) with bistrifluan and trifluanid has a good control effect on citrus red spider mites, showing good rapid effectiveness. 3 days after application, the control effects of 24% compound of formula (I)·bistrifluan suspension (21+3) and 36% compound of formula (I)·trifluanid suspension (30+6) on citrus red spider mites were 94.37% and 95.18%, respectively. At the 0.05 level, through difference analysis, the mixed preparation of the compound of formula (I) with bistrifluan and trifluanid was significantly higher than the control single agent.

[0202] Table 6 Control effect of each treatment on citrus red spider mites 3 days after application

[0203]

[0204] Note: The protective effect (%) in the table is the average value of each replicate, and the difference is at the 0.05% level. The same applies to the following tables.

[0205] As can be seen from Table 7, as time goes by, the control effect of the compound of formula (I) with bistrifluan and trifluanid on citrus red spider mites increases and the effective period is long. 7 days after application, the control effects of 24% compound of formula (I)·bistrifluan suspension (21+3) and 36% compound of formula (I)·trifluanid suspension (30+6) are 95.95% and 96.48%, respectively. At the 0.05 level, through difference analysis, at the 0.05 level, the mixed preparation of the compound of formula (I) with bistrifluan and trifluanid is significantly higher than the control single agent.

[0206] Table 7 Control effect of each treatment on citrus red spider mites 7 days after application

[0207]

[0208]

[0209] Note: The protective effect (%) in the table is the average value of each replicate, and the difference is at the 0.05% level.

[0210] Through indoor toxicity determination and field tests on citrus, the acaricidal composition prepared by compounding the compound of formula (I) of the present invention with either triflumuron or trifluanid has a good control effect on phytophagous pest mites.

[0211] The compounded acaricidal composition or the preparation thereof of the present invention has significant control effect, and is superior to a single agent in delaying the generation of drug resistance and prolonging the lasting effect. In addition, no compound agent was found to cause phytotoxicity to crops in the test, indicating that the acaricidal composition or preparation obtained can reduce production costs and use costs and is safe for crops when the acaricidal synergistic synergy is improved.

[0212] Although the present invention has been described in detail above with general instructions and specific implementation schemes, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A mite-killing composition, characterized in that: The active ingredients of the acaricide composition include active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I): The active ingredient B is any one of triflumuron or trifluanid.

2. The acaricidal composition according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:30 to 24:

1.

3. The acaricidal composition according to claim 1, characterized in that: The mass ratio of the compound of formula (I) to bistrifluan is 1:25-24:1; the mass ratio of the compound of formula (I) to triflumuron is 1:26-21:

1.

4. The acaricidal composition according to claim 1, characterized in that: Based on the total weight of the acaricidal composition being 100 wt %, the sum of the contents of the active ingredient A and the active ingredient B in the acaricidal composition is 1 to 80 wt %; Preferably, the sum of the contents of the compound of formula (I) and bistrifluan in the acaricidal composition is 2-50 wt %; the sum of the contents of the compound of formula (I) and triflumuron in the acaricidal composition is 2-60 wt %.

5. The acaricidal composition according to claim 1, characterized in that: The acaricide composition includes agriculturally acceptable auxiliary ingredients in addition to the active ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers.

6. The acaricidal composition according to claim 5, characterized in that: The acaricidal composition can be prepared into a formulation form permitted in agriculture, and the formulation form is selected from a solid formulation and / or a liquid formulation.

7. The acaricidal composition according to claim 6, characterized in that: The solid preparation is selected from wettable powders and water-dispersible granules; the liquid preparation is selected from soluble solutions, emulsifiable concentrates, aqueous emulsions, suspensions, suspoemulsions, microemulsions, and dispersible oil suspensions.

8. Use of the acaricidal composition according to any one of claims 1 to 7 for controlling harmful mites.

9. The use according to claim 8, characterized in that: The harmful mites are herbivorous harmful mites; preferably, the herbivorous harmful mites are Panonychus citri, Tetranychus citri, Tetranychus cinnabarinus, Tetranychus urticae or Tetranychus urticae.

10. The use according to claim 8, characterized in that: The acaricidal composition and / or its preparation is applied at an effective dose to the harmful mites to be controlled or the medium where they grow.

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