Detection device and detection method for strain culture

Abstract

The invention discloses a detection device and a detection method for strain culture, and belongs to the technical field of strain culture. The strain culture detection device comprises a box body and a box cover, a culture cabin is arranged at the inner bottom of the box body, and one or more sampling disc groups are arranged at the inner bottom of the box body; an extraction structure matched with the sampling disc group is arranged at the lower end of the box cover. According to the culture box, the box body and the box cover which are matched to form a closed state are arranged, and strains are cultured in the culture box and are not interfered by the outside; sampling is carried out from the inside of the box body, and the box body is closed again after sampling is completed, so that the whole operation is slightly interfered by external factors; the sampling disc group with a multi-layer form is arranged, so that multiple layers can be sampled at the same time; a plurality of sampling disc groups can be selectively arranged, so that multiple positions can be sampled at the same time, sampling points are further enriched, and the reliability and effectiveness of detection data are ensured; the sampling tray is provided with the central groove, and the strain preferentially covers the central groove in the culture process, so that the strain is relatively concentrated, and detection is facilitated.

Description

Technical Field

[0001] The present invention relates to the technical field of bacterial culture, and in particular to a detection device and a detection method for bacterial culture. Background Art

[0002] In the agricultural field, the application of beneficial bacteria is of vital importance. Specific beneficial bacteria can play a significant role in soil improvement, creating a more suitable soil environment for crop growth through various means such as improving soil structure, regulating soil pH, and improving soil fertility. At the same time, it also shows extremely excellent effects in promoting crop yields, which can improve crop yields and quality, and bring considerable economic benefits to agricultural production. In view of this, these beneficial bacteria have been widely used in agricultural production.

[0003] In the production process of related strains, it is very important to accurately grasp the strain culture conditions and ensure the final culture quality. In order to achieve this goal, it is necessary to test the strain culture regularly or irregularly. During the culture process, since the growth state of the strain may change with time and environmental changes, it is often necessary to perform multiple sampling operations in order to understand the culture dynamics of the strain in a timely manner.

[0004] In the prior art, the method commonly used for the culture detection of bacterial species is to directly sample from the surface of the culture structure such as the incubator, and then perform detection and analysis. However, this sampling method has great limitations. First, its sampling method is extremely single, and can only obtain bacterial samples on the surface of the incubator. It is impossible to perform multi-level sampling inside the incubator, and it is difficult to fully reflect the actual cultivation situation of the bacterial species at different depths and positions. Secondly, when sampling, if the incubator is opened for a long time, the bacterial species inside the incubator will come into contact with the external bacteria; external bacteria may enter the incubator and contaminate the bacterial species. This contamination will not only affect the normal growth of the bacterial species, but may also cause the mutation or death of the bacterial species. In addition, contamination will have a serious impact on the test results, and may even cause a large error between the test results and the true results, thereby affecting the accurate judgment of the bacterial species culture situation and subsequent production decisions.

[0005] Therefore, the applicant has made improvements. Summary of the invention

[0006] The purpose of the present invention is to solve the above problems in the prior art and to propose a detection device and method for bacterial culture.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions: A detection device for bacterial culture comprises a box body and a box cover; a culture chamber is arranged at the inner bottom of the box body and one or more sampling disc groups are placed thereon; an extraction structure cooperating with the sampling disc group is arranged at the lower end of the box cover.

[0008] In some embodiments, the sampling tray set is composed of: a vertically arranged central rod, and a multi-layer sampling tray coaxially arranged and fixedly connected to the central rod.

[0009] In some embodiments, the extraction structure includes: an inverted cartridge; The bottom layer of the sampling disk group is provided with a magnetic disk or a magnetic ring; the lower end of the inverted cylinder is provided with a magnetic bottom ring matched with the magnetic disk or the magnetic ring.

[0010] In some embodiments, the extraction structure further includes: one or more telescopic rods installed on the inner bottom of the box cover; a cylinder seat is fixedly provided at the lower end of the telescopic rod; and the lower end of the cylinder seat is connected to the inverted cylinder.

[0011] In some embodiments, the cartridge seat is magnetic; and a magnetic sheet is disposed on the top of the inverted cartridge.

[0012] In some embodiments, an equipment cabin with increased internal dimensions is disposed above the culture cabin; the equipment cabin is connected to the culture cabin, and a positioning step is formed at the connection; A reel cabin is arranged on one side of the equipment cabin, a reel cabin is arranged inside the reel cabin, and a sealing cloth is wound around the reel cabin.

[0013] In some embodiments, a clamping mechanism is provided in the equipment compartment; The clamping mechanism comprises: lower pressing bars respectively arranged corresponding to the positioning steps on the four sides; the lower pressing bars are vertically driven by a second telescopic rod arranged on the inner wall of the equipment cabin.

[0014] In some embodiments, one or more sterilization components are disposed in the equipment compartment.

[0015] A method for detecting bacterial culture, comprising the following steps: S1: Preparation of bacterial culture; S2, bacterial culture process; S3, bacterial sampling; S4. Bacterial strain detection.

[0016] In some embodiments, in step S1, the following steps are included: Clean the device and sterilize the culture chamber and equipment chamber through the sterilization component to ensure a clean and sterile environment inside.

[0017] In some embodiments, in step S2, the following steps are included: Put the bacteria into the culture chamber and place the sampling tray group accordingly; After that, wait for the bacteria to be cultured to the time point required for sampling and testing.

[0018] In some embodiments, in step S2, the culture chamber is closed or connected to the equipment chamber.

[0019] In some embodiments, in step S5, the following steps are included: Ensure that the closing cloth is in the storage state; Control the extraction structure to descend and cooperate with the sampling disc group; After the extraction structure drives the sampling plate group to rise to the top, the sealing cloth is controlled to move and cooperate with the pressing mechanism to temporarily seal the culture chamber; After that, open the box cover and remove the inverted cylinder.

[0020] Compared with the prior art, the present invention provides a detection device and a detection method for bacterial culture, which have the following beneficial effects.

[0021] 1. The present invention is provided with a box body and a box cover that cooperate to form a closed state, and the bacteria are cultured inside without being disturbed by the outside world; samples are taken from the inside of the box body, and the box body is sealed again after the sampling is completed, and the overall operation is less disturbed by the outside world; a sampling tray group with a multi-layer morphology is provided, and multiple layers can be sampled at the same time; multiple sampling tray groups can be optionally provided, and multiple samples can be taken at the same time, further enriching the sampling points and ensuring the reliability and effectiveness of the detection data; a central groove is provided on the sampling tray, and the bacteria preferentially cover the central groove during the cultivation process, which is relatively more concentrated and convenient for detection.

[0022] 2. The present invention sets an inverted cylinder to cover and wrap the sampling trays of each layer, and then takes out the sampling tray group to form a closed shape, which further reduces the influence of the external environment and improves the accuracy of the data; the bottom layer of the sampling tray group is provided with a magnetic disk or a magnetic ring, and the lower end of the inverted cylinder is provided with a magnetic bottom ring, which is automatic and convenient and efficient; a flexible sealing gasket / ring is provided at the lower end of the magnetic bottom ring, and the magnetic disk becomes the bottom cover, making the inverted cylinder a sealing structure, and the sampled colony is sealed, with a good sealing effect.

[0023] 3. In the present invention, during the upward sampling process, the box cover is in a closed state in coordination with the box body, and there will be no external environmental interference to affect the sampling; the cylinder seat is coordinated with the magnetic suction sheet to facilitate the rapid removal of the inverted cylinder connected to the sampling plate group; a temporary sealing component is provided to further reduce the impact on the culture chamber during sampling and testing; and a clamping mechanism is provided to improve the sealing of the temporary closure.

[0024] 4. In the present invention, the sealing cloth has a three-layer structure, including: outer gauze, filter cloth and inner gauze from outside to inside, which has reliable and effective bacterial filtration effect and also has ventilation function; a sterilization component is provided to treat the inside; the ultraviolet sterilization lamp is movable for better coverage.

[0025] Other advantages, objectives and features of the present invention will be described in part in the following description; and in part, will be apparent to those skilled in the art based on an examination of the following; or, may be taught from the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a schematic diagram of the structure of the present invention.

[0027] Figure 2 It is a cross-sectional structural schematic diagram of the present invention.

[0028] Figure 3 It is a schematic diagram of the structure of the box.

[0029] Figure 4 It is a schematic diagram of the cross-sectional structure of the box.

[0030] Figure 5 It is a schematic diagram of the cross-sectional structure of the box from top view.

[0031] Figure 6 This is a schematic diagram of the structure of the sampling barrel.

[0032] Figure 7 It is a schematic diagram of the structure of the sampling plate group.

[0033] In the figure: 1. Box body; 2. Culture chamber; 3. Equipment cabin; 4. Reel cabin; 5. sampling tray set; 51. center rod; 52. sampling tray; 53. magnetic disk; 6. Position the steps; 7. Reeling shaft; 71. Closing cloth; 72. Reeling motor; 8. Clamping mechanism; 81. Second telescopic rod; 82. Lower pressing strip; 9. Inner groove; 10. Sterilization component; 101. First motor; 102. Second screw rod; 103. Lamp holder; 104. Ultraviolet sterilization lamp; 11. Box cover; 12. Telescopic rod; 13. Cylinder seat; 14. Inverted cylinder; 141. Cylinder body; 142. Magnetic bottom ring; 143. Magnetic sheet; 15. Guide groove; 16. Guide rod; 17. Moving slot; 18. Second motor; 19. First screw rod; 20. Pull rod; 21. Positioning groove; 22. Positioning rod. DETAILED DESCRIPTION

[0034] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0035] Reference Figure 1-7 A detection device for bacterial culture includes a box body 1 and a box cover 11 that cooperate to form a closed state; bacterial culture is carried out in the box body 1, and the box cover 11 cooperates with the box body 1 to perform operations such as sampling and detection of the bacterial species.

[0036] like Figure 1 As shown, the box body 1 and the box cover 11 are independent structures; when the box cover 11 is closed from top to bottom, the two form a closed shape, and the bacteria can be cultured inside without being disturbed by the outside world; when sampling and testing are required, samples are taken from the inside of the box body 1 through the box cover 11; and after the sampling is completed, it is closed again; the bacteria can continue to be cultured in the box body 1.

[0037] In some embodiments, a culture chamber 2 is provided at the inner bottom of the box body 1, and one or more sampling tray groups 5 with a multi-layer morphology are placed in the culture chamber 2; at the same time, an extraction structure that cooperates with the sampling tray group 5 is provided at the lower end of the box cover 11 to lift the sampling tray group 5 and take it out to the outside.

[0038] like Figure 2 , 4 As shown; two sampling tray groups 5 for detecting bacterial species are placed inside the culture chamber 2.

[0039] It should be noted that the extraction structure has a first form away from the sampling plate group 5, and a second form approaching the sampling plate group 5 and removing it; in the first form, the extraction structure and the sampling plate group 5 do not interfere with each other, and the bacteria reproduce naturally on the sampling plate group 5; in the second state, the two cooperate to take out the sampling plate group 5 for testing.

[0040] Specifically, Figure 2 , 7 As shown; in order to form a multi-level structure, the sampling tray group 5 consists of: a vertically arranged center rod 51, and a multi-layer sampling tray 52 coaxially arranged and fixedly connected to the center rod 51; the spacing and height settings of the sampling tray 52 are set according to actual sampling needs.

[0041] It is understandable that the bottommost sampling tray 52 serves as a base to form a stable support.

[0042] like Figure 7 As shown; the sampling tray group 5 includes three sampling trays 52 arranged from top to bottom; the outer ring side of the bottom sampling tray 52 is provided with a magnetic ring.

[0043] Preferably, a central groove is provided on the sampling tray 52, which is more convenient for inoculation, placement, and aggregation culture of bacterial strains. In addition, during the culture process, the bacterial strains preferentially cover the central groove, which is relatively more concentrated and easier to detect.

[0044] Accordingly, the extraction structure includes: an inverted cylinder 14.

[0045] The inverted cylinder 14 includes a cylinder body 141 with an open bottom and a closed top.

[0046] When preparing for detection, the inverted cylinder 14 moves downward to cover and enclose the sampling trays 52 of each layer, and then moves upward to bring the sampling tray group 5 out of the culture chamber 2.

[0047] It is understandable that the inner diameter of the inverted cylinder 14 should be larger than the outer diameter of the sampling tray 52 “except the bottom layer” to ensure smooth downward falling.

[0048] Accordingly, we also need to set up a matching connection structure between the two (the inverted cylinder 14 and the sampling disk group 5) to achieve synchronous movement; considering the built-in nature of the sampling disk group 5 (placed in a closed box), and in order to form a form that does not require manual operation and reduce external interference; we set up a magnetic suction form to achieve adaptive matching between the extraction structure and the sampling disk group 5.

[0049] Specifically, a magnetic disk 53 or a magnetic ring is disposed at the bottom layer of the sampling disk group 5 ; correspondingly, a magnetic bottom ring 142 cooperating with the magnetic disk 53 or the magnetic ring is disposed at the lower end of the inverted cylinder 14 .

[0050] It can be understood that when set to the disk 53 form, the disk structure is located below the bottom sampling tray 52 and does not affect the culture of bacteria on the bottom sampling tray 52; when set to the magnetic ring form, the magnetic ring structure is located on the outer ring side of the bottom sampling tray 52.

[0051] In addition, it should be noted that the magnetic disk 53 or the magnetic ring is made of a material that is inherently magnetic; or, a material that is non-magnetic and can be attracted by the magnetic bottom ring 142 ; in short, as long as it can achieve coordinated attraction with the magnetic bottom ring 142 , it will be fine.

[0052] Furthermore, a flexible sealing gasket / ring is provided at the lower end of the magnetic bottom ring 142 .

[0053] When in the mating state, the magnetic bottom ring 142 absorbs the magnetic disk 53 and squeezes the sealing gasket / ring in the middle; the magnetic disk 53 becomes the bottom cover, making the inverted cylinder 14 a sealed structure, and the sampled bacterial colony is sealed; thereafter, the sampling disk group 5 is taken out along with the extraction structure for detection.

[0054] It is understandable that the sampling plate set 5 has a certain weight; in normal state, the sampling plate set 5 is in a stable position; after the extraction structure approaches, the magnetic bottom ring 142 and the magnetic disk 53 attract each other and absorb the sampling plate set 5.

[0055] It should be noted that the adsorption force between the magnetic bottom ring 142 and the magnetic disk 53 or the magnetic ring should be greater than the weight of the sampling disk set 5 to ensure that it is always in a stable and effective adsorption state during the movement.

[0056] Furthermore, the magnetic bottom ring 142 is in an electromagnetic form; it can be controlled to have magnetic force, or lose magnetic force according to the operating status, which is more flexible and convenient; after the sampling plate group 5 is tested, the sampling plate group 5 can be conveniently put back into the culture chamber 2 to continue the culture.

[0057] It should be noted that the sampling disc set 5 is placed at a designated position of the culture chamber 2, corresponding to the extraction structure, to achieve adsorption.

[0058] Preferably, a mark is provided on the inner bottom surface of the culture chamber 2 to assist in the alignment and placement of the sampling tray set 5 .

[0059] In some embodiments, further, we provide a moving component for the extraction structure to achieve switching between two forms of the extraction structure.

[0060] Specifically, the extraction structure also includes: one or more telescopic rods 12 installed on the inner bottom of the box cover 11; a cylinder seat 13 is fixedly provided at the lower end of the telescopic rod 12; the lower end of the cylinder seat 13 is connected to the inverted cylinder 14; the inverted cylinder 14 is driven by the telescopic rod 12 to move up and down.

[0061] Preferably, the telescopic rod 12 is electrically operated to facilitate direct control from the outside.

[0062] It should be noted that the telescopic rod 12 and the cylinder seat 13 correspond to the inverted cylinder 14 one by one; and each telescopic rod 12 is independently controlled; when performing sampling and testing, one sampling plate group 5 or multiple sampling plate groups 5 can be taken out.

[0063] It should also be noted that during the upward sampling process, the box cover 11 is in a closed state in cooperation with the box body 1, and there will be no external environmental interference to affect the sampling; Figure 6As shown, one extraction structure is in a downward position, and the other extraction structure is in an upward position; in this step, the inverted cylinder 14 and the sampling plate group 5 are matched inside a closed box, and the inverted cylinder 14 and the sampling plate group 5 are also in a closed form, and the bacteria to be detected are sealed and temporarily stored.

[0064] Of course, the box cover 11 needs to be removed afterwards to take out the sampling disc set 5 (the sampling disc set 5 and the inverted cylinder 14 are together).

[0065] In some embodiments, in order to further reduce factors such as contamination and environmental interference before reaching the detection, we set the sampling plate group 5 and the inverted cylinder 14 to an integrated sampling form; therefore, we need to control the connection state of the inverted cylinder 14 and the cylinder seat 13.

[0066] Specifically, the cartridge holder 13 is magnetic; a magnetic sheet 143 is disposed on the top of the inverted cartridge 14; the magnetic sheet 143 is adsorbed by the cartridge holder 13; and the inverted cartridge 14 connected to the sampling disk set 5 can be removed quickly and conveniently.

[0067] It is understandable that the cartridge holder 13 may be embedded with a magnetic structure such as a magnet; or, the cartridge holder 13 itself may be made of a magnetic material; in addition, the attraction between the cartridge holder 13 and the magnetic sheet 143 should be greater than the overall gravity of the inverted cartridge 14 and the sampling disc assembly 5, so that the cartridge holder 13 will not fall when not pulled by an external force.

[0068] Therefore, after removing the box cover 11, we can directly remove the inverted cylinder 14 (apply external force to overcome the adsorption force and remove the inverted cylinder 14 from the cylinder seat 13).

[0069] Preferably, the cartridge seat 13 is in the form of a lower groove with a groove at the bottom adapted to fit the top of the inverted cartridge 14; after the inverted cartridge 14 is inserted, the fit is tight.

[0070] In some embodiments, in order to further reduce the impact on the culture chamber 2 during sampling and testing, we also set up a temporary sealing component.

[0071] Specifically, an equipment cabin 3 with increased internal dimensions is arranged above the culture cabin 2; the equipment cabin 3 is connected to the culture cabin 2, and a positioning step 6 is formed at the connection; at the same time, a reel cabin 4 is arranged on one side of the equipment cabin 3, and the reel cabin 4 is connected to the equipment cabin 3 through a reel channel; a reel cabin 7 is arranged inside the reel cabin 4, and a sealing cloth 71 is wound on the reel 7, and a reel motor 72 is arranged at one end of the reel 7 for driving; the sealing cloth 71 passes through the reel channel and enters above the positioning step 6, and extends to the inner wall of the equipment cabin 3, thereby realizing temporary closure of the culture cabin 2 below.

[0072] It is understandable that the reel 7 drives the closing cloth 71 to shrink and coil; however, the extension and covering of the closing cloth 71 requires another power component.

[0073] Specifically, the end of the closing cloth 71 is fixed on the pull rod 20; on the front or rear inner wall of the equipment cabin 3, corresponding to the upper position of the positioning step 6, there is provided: a moving groove 17 opened along the moving direction of the closing cloth 71; a second motor 18 is arranged in the moving groove 17, and the output end of the second motor 18 is connected to a rotatable first screw rod 19; a screw hole is provided at the end of the pull rod 20, which cooperates with the first screw rod 19 for transmission; driven by the first screw rod 19, the pull rod 20 is driven to extend with the closing cloth 71.

[0074] It can be understood that when the pull rod 20 moves to the end of the first screw rod 19 , the closing cloth 71 covers the culture chamber 2 .

[0075] It should be noted that a movable groove 17 can be provided on each of the front and rear inner walls of the equipment compartment 3, and the second motors 18 in the movable grooves 17 on both sides are driven synchronously to provide stable and reliable pulling force.

[0076] Of course, it is also possible to configure one side as a motor-driven first screw rod 19 and the other side as a smooth rod slide rod. Figure 5 As shown; the equipment compartment 3 is only provided with a movable groove 17 on one side, and a positioning groove 21 is correspondingly provided on the other side; a positioning rod 22 (a smooth rod sliding rod) penetrating the pull rod 20 is provided inside the positioning groove 21; the positioning rod 22 cooperates with the first screw rod 19.

[0077] In some embodiments, in order to further enhance the temporary sealing effect of the sealing cloth 71 , a pressing mechanism 8 is also provided.

[0078] Specifically, a clamping mechanism 8 is provided in the equipment cabin 3 .

[0079] The clamping mechanism 8 includes lower pressing strips 82 respectively arranged corresponding to the positioning steps 6 on the four sides.

[0080] The lower pressure bar 82 is driven vertically by a second telescopic rod 81 arranged on the inner wall of the equipment cabin 3 .

[0081] When closing is required, after the pull strip 20 drives the closing cloth 71 to the end, the lower pressing strip 82 is pressed down by the second telescopic rod 81 to press the closing cloth 71 onto the positioning step 6; the four lower pressing strips 82 can press the four edges of the closing cloth 71 to improve the sealing performance of the temporary closure.

[0082] In some embodiments, we further design the shape of the sealing cloth 71 .

[0083] Specifically, the sealing cloth 71 is a three-layer structure, including: outer gauze, filter cloth and inner gauze from outside to inside; it is set as a three-layer structure, the filter cloth is sandwiched between the inner and outer layers, and it is controlled to have reliable and effective bacterial filtration effect; it also has a ventilation function.

[0084] In some embodiments, considering the treatment of the culture environment, we also set up a sterilization component 10.

[0085] Specifically, one or more sterilization components 10 are arranged in the equipment cabin 3 .

[0086] The sterilization component 10 includes: an ultraviolet sterilization lamp 104 .

[0087] Preferably, the ultraviolet germicidal lamps 104 are arranged along the front-to-back direction.

[0088] Furthermore, the ultraviolet germicidal lamp 104 is movable for better coverage.

[0089] It can be understood that the ultraviolet germicidal lamp 104 is arranged along the front-to-back direction and moves along the left-to-right direction.

[0090] Specifically, an inner groove 9 is opened on one side of the equipment compartment 3 in the length direction, and a driving assembly is arranged inside the inner groove 9; the ultraviolet sterilization lamp 104 is installed on the lamp holder 103, and the lamp holder 103 is connected to the driving assembly by transmission; the lamp holder 103 is driven to move by the driving assembly.

[0091] Preferably, an ultraviolet sterilization lamp 104 is provided on each of the left and right sides of the equipment cabin 3, and is independently controlled for movement; preferably, two discontinuous inner grooves 9 are provided accordingly.

[0092] In some embodiments, the driving assembly includes: a first motor 101 installed in the inner groove 9, and a second screw rod 102 drivingly connected to the output end of the first motor 101; the second screw rod 102 is threadedly matched with the end of the lamp holder 103 for transmission.

[0093] Thus, the movement of the ultraviolet germicidal lamp 104 is controlled by the first motor 101; the two ultraviolet germicidal lamps 104 can move freely in the equipment cabin 3, and the disinfection range is fully covered with good effect.

[0094] At the same time, a guide groove 15 is provided on the inner wall of the equipment compartment 3 away from the inner groove 9 , and the guide groove 15 corresponds to the inner groove 9 ; a guide rod 16 (a smooth rod sliding rod) penetrating the lamp holder 103 is provided inside the guide groove 15 .

[0095] It should be noted that the sterilization component 10 is arranged above the pull strip 20 and does not affect the pull strip 20 and does not interfere with the movement of the sealing cloth 71 ; at the same time, the sterilization component 10 should cooperate with the lower pressing strip 82 to avoid.

[0096] like Figure 4As shown, when the sterilization component 10 is moving, the lower pressing bar 82 cannot move up and down; accordingly, when the lower pressing bar 82 moves up and down, the sterilization component 10 should move to the avoidance position. Similarly, when the inverted cylinder 14 moves, the sterilization component 10 should move to the avoidance position.

[0097] Specifically, if the sterilization component 10 does not move to the left or right end, the two lower pressing strips 82 on the left and right sides will not interfere with the sterilization component 10; at the same time, the length of the front and rear lower pressing strips 82 should be less than the distance between the two sterilization components 10 in the avoidance state; Figure 4 In the avoidance state shown, the lower pressing bars 82 on the front and rear sides can move up and down.

[0098] A method for detecting bacterial culture, using the aforementioned device for detecting bacterial culture; It includes the following steps: S1: Preparation of bacterial culture; S2, bacterial culture process; S3, bacterial sampling; S4. Bacterial strain detection.

[0099] In step S1, the following steps are included: The device is cleaned, and the culture chamber 2 and the equipment chamber 3 are sterilized by the sterilization component 10 to ensure a clean and sterile environment inside.

[0100] In step S2, the following steps are included: The culture chamber 2 is filled with bacterial strains, and a sampling plate group 5 is placed correspondingly; After that, wait for the bacteria to be cultured to the time point required for sampling and testing.

[0101] Among them, in step S2, the culture cabin 2 forms a closed state, or is connected with the equipment cabin 3.

[0102] In step S3, the following steps are included: Ensure that the closing cloth 71 is in the storage state; Control the extraction structure to descend and cooperate with the sampling disc group 5; After the extraction structure drives the sampling plate group 5 to rise to the top, the sealing cloth 71 is controlled to move to cooperate with the pressing mechanism 8 to temporarily seal the culture chamber 2; Afterwards, the box cover 11 is opened and the inverted cylinder 14 is removed.

[0103] In step S4, the following steps are included: The inverted cylinder 14 is taken away for testing along with the sampling disc set 5 .

[0104] It should be noted that: the sampling plate group 5 is placed at a designated position at the bottom of the culture chamber 2 so that it can cooperate with and adsorb with the inverted cylinder 14; after the bacteria are cultured in the culture chamber 2 for a period of time, a part of the bacteria falls on the sampling plate group 5 (it can also be directly inoculated on the sampling plate group 5); when the extraction structure drives the sampling plate group 5 to rise to the top, its lower end should be higher than the sealing cloth 71.

[0105] In the present invention, a box body 1 and a box cover 11 are provided to form a closed state, and the bacteria are cultured inside without being disturbed by the outside world; samples are taken from the inside of the box body 1, and the box body is sealed again after the sampling is completed, and the overall operation is less disturbed by the outside world; a sampling plate group 5 with a multi-layer morphology is provided, and multiple layers can be sampled at the same time; multiple sampling plate groups 5 can be optionally provided, and multiple places can be sampled at the same time, further enriching the sampling points and ensuring the reliability and effectiveness of the detection data; a central groove is provided on the sampling tray 52, and the bacteria preferentially cover the central groove during the cultivation process, which is relatively more concentrated and convenient for detection; an inverted cylinder 14 is provided to cover and wrap the sampling trays 52 of each layer inside, and then the sampling plate group 5 is taken out to form a closed shape, further reducing the influence of the external environment and improving the accuracy of the data; the bottom layer of the sampling plate group 5 is provided with a magnetic disk 53 or a magnetic ring, and the lower end of the inverted cylinder 14 is provided with a magnetic bottom ring 142, which automatically Docking is convenient and efficient; a flexible sealing gasket / ring is arranged at the lower end of the magnetic bottom ring 142, and the magnetic disk 53 becomes the bottom cover, making the inverted cylinder 14 a sealing structure, and the sampled colony is sealed, and the sealing effect is good; in the upward sampling process, the box cover 11 is in a closed state in cooperation with the box body 1, and there will be no external environmental interference and influence on the sampling; the cylinder seat 13 cooperates with the magnetic suction sheet 143 to facilitate the quick removal of the inverted cylinder 14 connected to the sampling disk group 5; a temporary sealing component is set to further reduce the impact on the culture chamber 2 during sampling and detection; a clamping mechanism 8 is set to improve the sealing of the temporary closure; the sealing cloth 71 is a three-layer structure, including: outer gauze, filter cloth and inner gauze from outside to inside, which has reliable and effective bacterial filtration effect, and also has ventilation function; a sterilization component 10 is set to treat the inside; the ultraviolet sterilization lamp 104 is movable for better coverage.

[0106] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

[0107] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0108] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A detection device for bacterial culture, characterized in that: The invention comprises a box body (1) and a box cover (11); a culture chamber (2) is arranged at the inner bottom of the box body (1) and one or more sampling tray groups (5) are placed thereon; an extraction structure cooperating with the sampling tray group (5) is arranged at the lower end of the box cover (11).

2. The detection device for bacterial culture according to claim 1, characterized in that: The sampling tray set (5) is composed of a vertically arranged central rod (51) and a multi-layer sampling tray (52) coaxially arranged and fixedly connected to the central rod (51).

3. The detection device for bacterial culture according to claim 1, characterized in that: The extraction structure comprises: an inverted cylinder (14); The bottom layer of the sampling disk group (5) is provided with a magnetic disk (53) or a magnetic ring; the lower end of the inverted cylinder (14) is provided with a magnetic bottom ring (142) that cooperates with the magnetic disk (53) or the magnetic ring.

4. The detection device for bacterial culture according to claim 3, characterized in that: The extraction structure further comprises: one or more telescopic rods (12) mounted on the inner bottom of the box cover (11); a cylinder seat (13) is fixedly provided at the lower end of the telescopic rod (12); and the lower end of the cylinder seat (13) is connected to the inverted cylinder (14).

5. The detection device for bacterial culture according to claim 4, characterized in that: The cylinder seat (13) is magnetic; and a magnetic attraction sheet (143) is provided on the top of the inverted cylinder (14).

6. The detection device for bacterial culture according to claim 1, characterized in that: An equipment cabin (3) with increased internal dimensions is provided above the culture cabin (2); the equipment cabin (3) is connected to the culture cabin (2), and a positioning step (6) is formed at the connection; A reel compartment (4) is provided on one side of the equipment compartment (3), a reel compartment (4) is provided inside with a reel-in shaft (7), and a sealing cloth (71) is wound around the reel-in shaft (7).

7. The detection device for bacterial culture according to claim 6, characterized in that: A pressing mechanism (8) is provided in the equipment cabin (3); The clamping mechanism (8) comprises: lower pressing bars (82) respectively arranged corresponding to the positioning steps (6) on the four sides; the lower pressing bars (82) are vertically driven by a second telescopic rod (81) arranged on the inner wall of the equipment cabin (3).

8. The detection device for bacterial culture according to claim 1, characterized in that: One or more sterilization components (10) are arranged in the equipment cabin (3).

9. A method for detecting bacterial culture, characterized in that: A detection device for bacterial culture according to any one of claims 1 to 8; The detection method comprises the following steps: S1: Preparation of bacterial culture; S2, bacterial culture process; S3, bacterial sampling; S4. Bacterial strain detection.

10. The method for detecting bacterial culture according to claim 9, characterized in that: In step S3, The following steps are involved: Ensure that the closing cloth (71) is in a stored state; Controlling the extraction structure to descend and cooperate with the sampling disc assembly (5); After the extraction structure drives the sampling plate group (5) to rise to the top, the sealing cloth (71) is controlled to move to cooperate with the pressing mechanism (8) to temporarily seal the culture chamber (2); Afterwards, open the box cover (11) and remove the inverted cylinder (14).

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