Micro-fluidic chip rapid detection device for drug resistance of blood flow infection sample

Abstract

The invention relates to the technical field of clinical sample detection, and provides a blood flow infection sample drug resistance micro-fluidic chip rapid detection device which comprises a detection box body, a micro-fluidic chip is arranged in the detection box body, a cleaning detection mechanism is arranged on the lower portion of the micro-fluidic chip in a sliding mode, the cleaning detection mechanism is connected with a driving mechanism, and the driving mechanism is connected with the micro-fluidic chip. The device further comprises a negative pressure mechanism, one end of the negative pressure mechanism is communicated with the interior of the detection box body, and the air outlet end of the negative pressure mechanism is connected with an unpowered sample pretreatment mechanism. The device has the advantages that the critical disease infection pathogenic bacterium identification and drug sensitivity detection period can be shortened, the survival rate of critical disease infection patients is increased, the device has certain linkage, and the overall structural design is ingenious.

Description

Technical Field

[0001] The present invention relates to the technical field of drug resistance detection and analysis devices, and in particular to a microfluidic chip rapid detection device for drug resistance of bloodstream infection samples. Background Art

[0002] The clinical pathogen identification and drug sensitivity testing process includes clinical sample collection, culture, separation and extraction, identification and drug sensitivity testing. During the sample culture and separation and extraction stages, there are problems such as pathogen detection dependence on culture, low-concentration pathogen enrichment, pathogen separation in complex samples, and drug sensitivity testing sample processing, and the detection sensitivity is low; the time required from clinical samples to drug sensitivity reports is at least 2 days, which is difficult to meet the needs of rapid diagnosis and precise treatment of critical infections, resulting in a significant increase in the mortality rate of drug-resistant bacterial infections.

[0003] In particular, the sample processing process takes a certain amount of time, so it is necessary to design a detection device that can quickly process the test samples in an integrated manner. Summary of the invention The present application proposes a microfluidic chip rapid detection device for bloodstream infection sample resistance, which can shorten the cycle of critical infection pathogen identification and drug sensitivity testing, and improve the survival rate of critical infection patients. The device has a certain degree of linkage and the overall structure design is ingenious.

[0004] To this end, the technical solution adopted in the present application is as follows: a bloodstream infection sample resistance microfluidic chip rapid detection device, including a detection box, a microfluidic chip is arranged inside the detection box, a cleaning detection mechanism is slidingly arranged at the lower part of the microfluidic chip, the cleaning detection mechanism is connected to the driving mechanism, and also includes a negative pressure mechanism, one end of the negative pressure mechanism is connected to the interior of the detection box, and the air outlet end of the negative pressure mechanism is connected to an unpowered sample pretreatment mechanism.

[0005] By adopting the above technical solution: the microfluidic chip is used to disperse the sample, and the cleaning detection mechanism has at least two functions. When the driving mechanism drives it to move, one is to clean the bottom of the microfluidic chip first, and the other is to perform imaging to make the imaging clearer; the negative pressure mechanism is used to vacuum the inside of the detection box. During the vacuuming process, the airflow can drive the unpowered sample pretreatment mechanism to pre-treat the sample. The airflow in the vacuuming process is used as power, which eliminates the need for installation of other power components and has good linkage.

[0006] Optionally, a sliding frame is provided inside the detection box, the sliding frame includes a vertical beam and a horizontal beam connected to the vertical beam, a slide rail is provided on the horizontal beam, the cleaning detection mechanism is slidably set on the slide rail, and the driving mechanism is set at the end of the slide rail.

[0007] By adopting the above technical solution, the cleaning detection mechanism can move on the slide rail under the drive of the driving mechanism, and perform the action process of cleaning first and then imaging.

[0008] Optionally, the microfluidic chip includes a body, the body is provided with a plurality of detection holes connected in sequence, the body is provided with an injection groove, and the injection groove is connected with one of the detection holes.

[0009] By adopting the above technical solution: the sample is injected into the injection groove after pretreatment, and then flows into each detection hole in turn, wherein the bottom of the detection hole is transparent.

[0010] Optionally, the cleaning detection mechanism includes a slider slidably disposed on a slide rail, and the slider is provided with an imaging component and a cleaning component.

[0011] By adopting the above technical solution: the imaging component can be a high-frequency camera, etc., and the cleaning component can clean the lower part of the chip body before imaging.

[0012] Optionally, the cleaning assembly includes an L-shaped mounting rod arranged on the slider, the upper end of the L-shaped mounting rod is provided with a cleaning cotton that contacts the bottom of the micro-control flow chip, and the driving mechanism includes an electric push rod arranged at the end of the beam.

[0013] By adopting the above technical solution: when the electric push rod drives the slider to move, the L-shaped mounting rod simultaneously moves with it, thereby driving the cleaning surface to move to clean the microfluidic chip.

[0014] Optionally, the negative pressure mechanism includes a vacuum pump disposed outside the detection box, an air inlet of the vacuum pump is connected to the interior of the detection box, and an air outlet of the vacuum pump is driven and connected to an unpowered sample pretreatment mechanism.

[0015] Optionally, the unpowered sample preprocessing mechanism includes a linkage component connected to the air outlet of the vacuum pump and a hybrid component arranged on the upper side of the linkage component.

[0016] Optionally, the linkage assembly includes a vibration shell arranged on the upper part of the detection box, a vibration assembly is provided inside the vibration shell, the hybrid assembly includes a mixing cup fixed on the upper side of the vibration shell, a filter is provided on the inner side of the mixing cup, and the lower side of the mixing cup is connected to the secondary filter cup at the upper part of the injection tank through a pipeline.

[0017] By adopting the above technical solution: the vibrating shell can generate vibration when air flows through it, thereby driving the mixing cup on its upper side to vibrate, and the sample in the mixing cup is mixed and filtered, and then it can flow to the secondary filter cup through the pipeline for secondary filtration.

[0018] Optionally, a bearing plate is provided on the upper part of the vibration shell, the mixing cup is fixed on the bearing plate, the vibration assembly includes a plurality of groups of upper elastic sheets and lower elastic sheets overlapped with each other in an arc shape, the other end of the upper elastic sheet is fixed on the bearing plate, and the other end of the lower elastic sheet is fixed on the bottom wall of the vibration shell; A pneumatic cavity is provided on the outer arc side of the overlapped part of the upper elastic sheet and the lower elastic sheet, and the pneumatic cavity is connected to the air outlet end of the negative pressure mechanism; An impact plate is also provided on the inner side of the upper elastic sheet, and a secondary pushing block is provided on the free end of the lower elastic sheet.

[0019] By adopting the above technical solution: during the vacuuming process, when the airflow passes through the pneumatic cavity, the upper elastic sheet and the lower elastic sheet are driven to expand outward, and the impact plate on the upper elastic sheet contacts the supporting plate to vibrate the supporting plate, thereby driving the box body to vibrate. When the vacuuming is completed, during the return of the upper elastic sheet and the lower elastic sheet, the secondary push block can push the upper elastic sheet again to move, forming a secondary vibration, thereby speeding up the sample separation process.

[0020] Optionally, the detection box is provided with an air intake passage with a switch function.

[0021] By adopting the above technical solution: when a larger airflow is needed, the air intake channel can be opened first, and then closed after the mixing process is completed.

[0022] The working principle and beneficial effects of this application are: 1. In the present application, the microfluidic chip is used to disperse samples, and the cleaning detection mechanism has at least two functions. When the driving mechanism drives it to move, one is that the bottom of the microfluidic chip can be cleaned first, and the other is that imaging can be performed to make the imaging clearer; the negative pressure mechanism is used to vacuum the inside of the detection box. During the vacuuming process, the airflow can drive the unpowered sample pretreatment mechanism to pretreat the sample. The airflow in the vacuuming process is used as power, which eliminates the installation of other power components and has good linkage. At the same time, it speeds up the progress of sample pretreatment, shortens the time of the overall detection process, and improves the detection efficiency.

[0023] 2. In the present application, during the vacuuming process, when the airflow passes through the pneumatic cavity, the upper elastic sheet and the lower elastic sheet are driven to expand outward, and the impact plate on the upper elastic sheet contacts the supporting plate, causing the supporting plate to vibrate, thereby driving the box body to vibrate. When the vacuuming is completed, during the return process of the upper elastic sheet and the lower elastic sheet, the secondary push block can push the upper elastic sheet again to move, forming a secondary vibration, thereby speeding up the sample separation process. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The present application is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0025] Figure 1 It is a schematic diagram of the overall structure of an embodiment of the present application; Figure 2 This is a schematic diagram of the structure of the microfluidic chip of an embodiment of the present application; Figure 3 This is a schematic diagram of the structure of the cleaning detection mechanism of an embodiment of the present application; Figure 4 This is a partial structural schematic diagram of the unpowered sample preprocessing mechanism according to an embodiment of the present application.

[0026] In the figure: 100, detection box; 110, sliding frame; 120, slide rail; 200, microfluidic chip; 210, detection hole; 220, injection slot; 300, cleaning detection mechanism; 310, slider; 320, imaging component; 330, L-shaped mounting rod; 340, cleaning cotton; 400, driving mechanism; 500, negative pressure mechanism; 600, unpowered sample pretreatment mechanism; 610, vibration shell; 620, mixing cup; 621, filter element; 630, secondary filter cup; 640, supporting plate; 650, upper elastic sheet; 660, lower elastic sheet; 670, pneumatic cavity; 680, impact plate; 690, secondary push block; 700, air inlet channel. DETAILED DESCRIPTION

[0027] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] like Figure 1-4 As shown, this embodiment proposes a bloodstream infection sample drug resistance microfluidic chip rapid detection device, including a detection box 100, a microfluidic chip 200 is arranged inside the detection box 100, a cleaning detection mechanism 300 is slidably provided at the lower part of the microfluidic chip 200, the cleaning detection mechanism 300 is connected to the driving mechanism 400, and also includes a negative pressure mechanism 500, one end of the negative pressure mechanism 500 is connected to the inside of the detection box 100, and the air outlet end of the negative pressure mechanism 500 is connected to an unpowered sample pretreatment mechanism 600.

[0029] The basic principle of this embodiment is as follows: the microfluidic chip 200 is used to disperse the sample, and the cleaning detection mechanism 300 has at least two functions. When the driving mechanism 400 drives it to move, one is that the bottom of the microfluidic chip 200 can be cleaned first, and the other is that imaging can be performed to make the imaging clearer; the negative pressure mechanism 500 is used to vacuum the inside of the detection box 100. During the vacuuming process, the airflow can drive the unpowered sample pretreatment mechanism 600 to act to pretreatment the sample. The airflow in the vacuuming process is used as power, which eliminates the need to install other power components and has good linkage.

[0030] Among them, a sliding frame 110 is provided inside the detection box 100, and the sliding frame 110 includes a vertical beam and a horizontal beam connected to the vertical beam. A slide rail 120 is provided on the horizontal beam. The cleaning detection mechanism 300 is slidably set on the slide rail 120, and the driving mechanism 400 is set at the end of the slide rail 120. The cleaning detection mechanism 300 can move on the slide rail 120 under the drive of the driving mechanism 400 to perform the action process of cleaning first and then imaging.

[0031] Reference Figure 2 The control chip in this embodiment includes a main body, on which are provided a plurality of detection holes 210 connected in sequence, and an injection groove 220 is provided on the main body, and the injection groove 220 is connected with one of the detection holes 210. After pretreatment, the sample is injected into the injection groove 220 and then flows into each detection hole 210 in sequence, wherein the bottom of the detection hole 210 is transparent.

[0032] Reference Figure 1 and Figure 3 The cleaning detection mechanism 300 in this embodiment includes a slider 310 slidably set on the slide rail 120, and the slider 310 is provided with an imaging component 320 and a cleaning component. The imaging component 320 can be a high-frequency camera, etc. The cleaning component can clean the lower part of the chip body before imaging.

[0033] The cleaning assembly includes an L-shaped mounting rod 330 arranged on the slider 310, and the upper end of the L-shaped mounting rod 330 is provided with a cleaning cotton 340 that contacts the bottom of the microfluidic chip. The driving mechanism 400 includes an electric push rod arranged at the end of the beam. When the electric push rod drives the slider 310 to move, the L-shaped mounting rod 330 also follows its movement, thereby driving the cleaning surface to move to clean the microfluidic chip 200.

[0034] The negative pressure mechanism 500 in this embodiment includes a vacuum pump disposed outside the detection box 100 , an air inlet of the vacuum pump is connected to the inside of the detection box 100 , and an air outlet of the vacuum pump is driven and connected to the unpowered sample pretreatment mechanism 600 .

[0035] Reference Figure 1 and Figure 4 The unpowered sample preprocessing mechanism 600 in this embodiment includes a linkage component connected to the air outlet of the vacuum pump and a hybrid component arranged on the upper side of the linkage component.

[0036] The linkage assembly includes a vibration shell 610 arranged on the upper part of the detection box 100, and a vibration assembly is arranged inside the vibration shell 610. The hybrid assembly includes a mixing cup 620 fixed on the upper side of the vibration shell 610, and a filter 621 is arranged on the inner side of the mixing cup 620. The lower side of the mixing cup 620 is connected to the secondary filter cup 630 at the upper part of the injection tank 220 through a pipeline.

[0037] The vibrating shell 610 can generate vibration when air flows through it, thereby driving the mixing cup 620 on its upper side to vibrate, and mixing and filtering the sample in the mixing cup 620, and then the sample can flow through the pipeline to the secondary filter cup 630 for secondary filtration.

[0038] A bearing plate 640 is provided on the upper part of the vibration housing 610, the mixing cup 620 is fixed on the bearing plate 640, and the vibration assembly includes a plurality of groups of upper elastic sheets 650 and lower elastic sheets 660 overlapped with each other in an arc shape, the other end of the upper elastic sheet 650 is fixed on the bearing plate 640, and the other end of the lower elastic sheet 660 is fixed on the bottom wall of the vibration housing 610; A pneumatic cavity 670 is provided on the outer arc side of the overlapped portion of the upper elastic sheet 650 and the lower elastic sheet 660, and the pneumatic cavity 670 is connected to the air outlet end of the negative pressure mechanism 500; An impact plate 680 is further disposed on the inner side of the upper elastic sheet 650 , and a secondary pushing block 690 is disposed at the free end of the lower elastic sheet 660 .

[0039] During the vacuuming process, when the airflow passes through the pneumatic cavity 670, it drives the upper elastic sheet 650 and the lower elastic sheet 660 to expand outward, and the impact plate 680 on the upper elastic sheet 650 contacts the supporting plate 640 to vibrate the supporting plate 640, thereby driving the box body to vibrate. When the vacuuming is completed, during the process of the upper elastic sheet 650 and the lower elastic sheet 660 returning to their original positions, the secondary push block 690 can push the upper elastic sheet 650 again to form a secondary vibration, thereby speeding up the sample separation process.

[0040] The detection box 100 in this embodiment is provided with an air intake channel 700 with a switch function. When a larger airflow is required, the air intake channel 700 can be opened first, and then closed after the mixing process is completed.

[0041] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A microfluidic chip rapid detection device for bloodstream infection sample drug resistance, characterized in that: The invention comprises a detection box (100), wherein a microfluidic chip (200) is arranged inside the detection box (100), a cleaning detection mechanism (300) is slidably arranged at the lower part of the microfluidic chip (200), wherein the cleaning detection mechanism (300) is connected to a driving mechanism (400), and further comprises a negative pressure mechanism (500), wherein an air inlet end of the negative pressure mechanism (500) is connected to the inside of the detection box (100), and an air outlet end of the negative pressure mechanism (500) is connected to an unpowered sample pretreatment mechanism (600).

2. The microfluidic chip rapid detection device for bloodstream infection sample drug resistance according to claim 1 is characterized in that: A sliding frame (110) is provided inside the detection box (100), the sliding frame (110) comprising a vertical beam and a horizontal beam connected to the vertical beam, a slide rail (120) is provided on the horizontal beam, the cleaning detection mechanism (300) is slidably arranged on the slide rail (120), and the driving mechanism (400) is arranged at the end of the slide rail (120).

3. The microfluidic chip rapid detection device for bloodstream infection sample drug resistance according to claim 1, characterized in that: The microfluidic chip (200) comprises a body, on which a plurality of detection holes (210) connected in sequence are provided, and on which an injection groove (220) is provided, wherein the injection groove (220) is connected to one of the detection holes (210).

4. The microfluidic chip rapid detection device for bloodstream infection sample drug resistance according to claim 2, characterized in that: The cleaning detection mechanism (300) comprises a sliding block (310) slidably arranged on a sliding rail (120), and an imaging component (320) and a cleaning component are arranged on the sliding block (310).

5. The microfluidic chip rapid detection device for bloodstream infection sample drug resistance according to claim 4 is characterized in that: The cleaning assembly comprises an L-shaped mounting rod (330) arranged on a slider (310), a cleaning cotton (340) in contact with the bottom of the micro-control flow chip being provided at the upper end of the L-shaped mounting rod (330), and the driving mechanism (400) comprises an electric push rod arranged at the end of a crossbeam.

6. The microfluidic chip rapid detection device for bloodstream infection sample drug resistance according to claim 3, characterized in that: The negative pressure mechanism (500) comprises a vacuum pump arranged outside the detection box (100), the air inlet end of the vacuum pump being in communication with the interior of the detection box (100), and the air outlet end of the vacuum pump being drivingly connected to the unpowered sample pretreatment mechanism (600).

7. The microfluidic chip rapid detection device for bloodstream infection sample drug resistance according to claim 6, characterized in that: The unpowered sample pre-processing mechanism (600) comprises a linkage component connected to the air outlet of a vacuum pump and a hybrid component arranged on the upper side of the linkage component.

8. The microfluidic chip rapid detection device for bloodstream infection sample drug resistance according to claim 7, characterized in that: The linkage assembly comprises a vibration housing (610) arranged on the upper part of the detection box (100), a vibration assembly is arranged inside the vibration housing (610), the hybrid assembly comprises a mixing cup (620) fixed on the upper side of the vibration housing (610), a filter element (621) is arranged inside the mixing cup (620), and the lower side of the mixing cup (620) is connected to a secondary filter cup (630) at the upper part of the injection tank (220) through a pipeline.

9. The microfluidic chip rapid detection device for bloodstream infection sample drug resistance according to claim 8, characterized in that: A bearing plate (640) is provided on the upper part of the vibration housing (610), the mixing cup (620) is fixed on the bearing plate (640), the vibration assembly comprises a plurality of groups of upper elastic sheets (650) and lower elastic sheets (660) in the shape of circular arcs, one end of which overlaps with each other, the other end of the upper elastic sheet (650) is fixed on the bearing plate (640), and the other end of the lower elastic sheet (660) is fixed on the bottom wall of the vibration housing (610); A pneumatic cavity (670) is provided on the outer arc side of the overlapped portion of the upper elastic sheet (650) and the lower elastic sheet (660), and the pneumatic cavity (670) is communicated with the air outlet end of the negative pressure mechanism (500); An impact plate (680) is also provided on the inner side of the upper elastic sheet (650), and a secondary pushing block (690) is provided at the free end of the lower elastic sheet (660).

10. The microfluidic chip rapid detection device for bloodstream infection sample drug resistance according to any one of claims 1 to 9, characterized in that: The detection box (100) is provided with an air intake channel (700) having a switch function.

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