Meat and egg type flower neck green shell poanpanjiang black-bone chicken complete set line seed production method

Abstract

The application discloses a kind of meat and egg dual-purpose type flower neck green shell Pu An Panjiang black-bone chicken matching system seed production method, the seed production method is introduced into the blood relationship of bamboo town chicken on the basis of blood relationship of Pu An Panjiang black-bone chicken, using matching combination ZX × [(MW × PA) × MW], the appearance characteristics of flower neck, black feather, black skin, black shank, black crown, black meat are obtained to the commodity generation of flower neck green shell Pu An Panjiang black-bone chicken matching system, appearance ideal, large body size, fast growth rate, early market age, high meat yield, produce jade green shell chicken egg, meat and egg dual-purpose, weight, growth rate, slaughter performance, reproductive performance, market age are all superior to Pu An Panjiang black-bone chicken pure line, simultaneously, the present application can accurately select appearance ideal, large body size, fast growth rate, early market age, high meat yield, produce jade green shell chicken egg, performance stable meat and egg dual-purpose type flower neck green shell Pu An Panjiang black-bone chicken matching system using individual selection and family selection method, is suitable for the popularization and application in poultry breeding technology field.

Description

TECHNICAL FIELD

[0001] The application relates to the field of poultry breeding technology, in particular to a method for breeding a meat-egg dual-purpose flower-neck green-shell Pu' anpanjiang black-bone chicken. BACKGROUND

[0002] Local chickens have the characteristics of excellent meat and egg quality, but the feeding cycle is long and the production performance is low. Improving the growth rate, shortening the market age and increasing the egg production can effectively reduce the comprehensive breeding cost of local chickens, which is one of the measures to reduce costs and increase benefits in response to the high cost of feed raw materials. At the same time, the unique eggshell color (such as the natural green color of green-shell eggs) not only meets the aesthetic needs of consumers for product differentiation, but also transmits the quality association of "high nutrition" and "ecological breeding" through visual cognition, so that the price of green-shell eggs can reach 2-4 times that of ordinary eggs. Therefore, improving the eggshell color has become a new way to improve the economic benefits of a limited number of egg products.

[0003] Pu' anpanjiang black-bone chicken is named after being mainly produced in the Beipanjiang River Basin of Pu'an County, southwest of Guizhou Province, and is also known as Pu'an Niu Jiaoshan black-bone chicken and Pu'an black-gold chicken. After long-term natural and artificial selection, it has gradually formed a black-bone chicken characteristic breed with meat and medicinal properties and resistance to roughage, and has the characteristics of black feathers, black shanks, black skin, black meat and black bones, strong resistance, fresh and fragrant meat and eggs, and has become the main promoted breed of local poultry industry. However, the current development and utilization of Pu' anpanjiang black-bone chicken is still very low, and the problems of loss of germplasm resources and lack of systematic protection mechanism are becoming increasingly serious, and there are problems such as slow growth rate, small body weight in early stage, long feeding cycle, low egg production, and small egg weight, which seriously restrict the high-quality development of the industry. Therefore, it is necessary to carry out germplasm innovation on local resources Pu' anpanjiang black-bone chicken.

[0004] Zhu Xiang chicken is named after being originally produced in Chishui City, Zunyi City, Guizhou Province, which is known as the "home of bamboo". It belongs to a meat-egg dual-purpose local breed. Due to the preference for black-bone individuals in the local area, it has been intentionally selected and retained, forming the appearance characteristics of black feathers, skin, meat, bones, beak, crown and toes. It has high egg production performance and some groups can produce green-shell eggs. It is a very precious and highly plastic breeding material, and its development and utilization is still relatively low. The black-feathered chicken has excellent meat and egg production performance, fast growth rate and large body weight, and has the appearance characteristics of red-feathered chicken, black-bone chicken, black-skin chicken and black-shank chicken. It can be used to improve the slow growth rate and low body weight of local breeds.

[0005] Therefore, by using the advantages of the above materials, further hybrid improvement can be carried out on Pu' anpanjiang black-bone chicken to shorten the market age, improve the egg production performance, and cultivate a meat-egg dual-purpose flower-neck green-shell Pu' anpanjiang black-bone chicken suitable for the needs of the southwest and southern regions. SUMMARY

[0006] The technical problem solved by the present application is to provide a meat-egg dual-purpose flower neck green shell Pu'anpanjiang black-bone chicken complete line breeding method with ideal appearance, wide body shape, fast growth speed, early market age, high meat yield, production of jade green shell eggs and stable performance.

[0007] The technical solution adopted by the present application to solve its technical problem is that the meat-egg dual-purpose flower neck green shell Pu'anpanjiang black-bone chicken complete line breeding method comprises the following steps:

[0008] 1) paternal line selection:

[0009] 101) Select the existing Zhuxiang chicken ZX line that meets the 8, 16-week-old weight standard and appearance standard of the male chicken and the 8, 16-week-old weight standard and 43-week-old egg production standard and appearance standard of the female chicken, and breed according to the 8, 16-week-old weight standard and appearance standard of the male chicken and the 8, 16-week-old weight standard and 43-week-old egg production standard and appearance standard of the female chicken to obtain the great-grandparent ZX paternal male and the great-grandparent ZX paternal female;

[0010] 102) Breeding with the great-grandparent ZX paternal male and the great-grandparent ZX paternal female, and selecting according to the 8, 16-week-old weight standard and appearance standard of the male chicken and the 8, 16-week-old weight standard and 43-week-old egg production standard and appearance standard of the female chicken to obtain the grandparent ZX paternal male and the grandparent ZX paternal female;

[0011] 103) Breeding with the grandparent ZX paternal male and the grandparent ZX paternal female, and selecting according to the 8, 16-week-old weight standard, appearance standard, 30-week-old male semen quality standard and green shell egg positive homozygous genotype to obtain the parent ZX paternal male;

[0012] 2) maternal line selection:

[0013] 201) Select the existing purebred black-feather chicken MW line that meets the 8, 16-week-old weight standard and appearance standard of the male chicken and the 8, 16-week-old weight standard and 43-week-old egg production standard and appearance standard of the female chicken, and breed according to the 8, 16-week-old weight standard and appearance standard of the male chicken to obtain the great-grandparent MW maternal male and the grandparent MW maternal female;

[0014] 202) Breeding with the existing purebred Pu'anpanjiang black-bone chicken PA line that meets the 8, 16-week-old weight standard and appearance standard of the male chicken and the 8, 16-week-old weight standard and 43-week-old egg production standard and appearance standard of the female chicken, and selecting according to the 43-week-old egg production standard and appearance standard of the female chicken to obtain the great-grandparent PA maternal female;

[0015] 203), the great-great-grandparent ZX paternal male and the great-great-grandparent ZX maternal female are mated, and the offspring are selected according to the 8-week-old and 16-week-old weight standards of the male chickens and the appearance standards to obtain the grandparent MP maternal male;

[0016] 204), the grandparent MP maternal male and the grandparent MW maternal female are backcrossed, and the offspring are selected according to the 8-week-old and 16-week-old weight standards of the female chickens, the 43-week-old egg production standard, and the appearance standards to obtain the parent generation MM maternal female;

[0017] 3), the parent generation ZX paternal male obtained by paternal selection is mated with the parent generation MM maternal female obtained by maternal selection to obtain the Huajinglǜkepu'andianjiangwu chicken.

[0018] Further, in steps 101) to 103), the selection of the great-great-grandparent ZX paternal male, the great-great-grandparent ZX maternal female, the grandparent ZX paternal male, the grandparent ZX maternal female, and the parent generation ZX paternal male is carried out by single-cage feeding and artificial insemination.

[0019] Further, in steps 201) to 204), the selection of the great-great-grandparent MW maternal male, the great-great-grandparent PA maternal female, the grandparent MP maternal male, the grandparent MW maternal female, and the parent generation MM maternal female is carried out by single-cage feeding and artificial insemination.

[0020] Further, in steps 101) to 103), the 8-week-old weight standard of the male chicken of Zhuxiang chicken is 0.7 kg, the 16-week-old weight standard is 1.75 kg, the 43-week-old egg production standard is more than 80 eggs for the female chickens of the same family at the age of 43 weeks, the appearance characteristics of the male chicken of Zhuxiang chicken are robust body, solid constitution, dark green and golden luster of feathers, developed sickle feather, black feathers, black skin, black shanks, black comb, and black meat, the semen quality requirements of the male chicken of Zhuxiang chicken are more than 0.5 mL of semen collected at a time, milky white, thick, pH 7-8, density 30-50 billion / mL, viability 0.7 or more, and fertilization rate 90% or more; the 43-week-old egg production standard of the female chicken of Zhuxiang chicken is an average egg production of more than 80 eggs, green eggshell color, and an average egg weight of 50 g, the 8-week-old weight standard of the female chicken of Zhuxiang chicken is 0.65 kg, the 16-week-old weight standard is 1.3 kg, and the appearance characteristics of the female chicken of Zhuxiang chicken are slim body shape, uniform structure, black feathers, black skin, black shanks, black comb, and black meat.

[0021] Further, in step 201, the standard weight of the male chicken of the Ma-fei black-bone chicken is 1.5 kg at 8 weeks old, 2.4 kg at 16 weeks old, and the standard egg production at 43 weeks old is more than 85 eggs, which is the average egg production of the female chickens of the same family at 43 weeks old. The appearance of the male chicken of the Ma-fei black-bone chicken is as follows: large and strong body, deep red straight single comb, well-developed sickle feather, red and yellow all over the body, black wing and sickle feather, black skin, shank and meat. The quality of the semen of the male chicken of the Ma-fei black-bone chicken is required to be more than 0.7 mL per time, milky white, thick, pH 7-8, density 30-50 billion / mL, viability 0.7, and fertilization rate more than 90%. The standard weight of the female chicken of the Ma-fei black-bone chicken is 1 kg at 8 weeks old, 1.6 kg at 16 weeks old, and the standard egg production at 43 weeks old is more than 85 eggs with pink eggshell, and the average egg weight is 54 g. The appearance of the female chicken of the Ma-fei black-bone chicken is as follows: large and round body, deep red straight single comb, yellow all over the body, black skin, shank and meat.

[0022] Further, in step 202, the standard weight of the male chicken of the Pu'an Panjiang black-bone chicken is 0.5 kg at 8 weeks old, 1.4 kg at 16 weeks old, and the standard egg production at 43 weeks old is more than 65 eggs, which is the average egg production of the female chickens of the same family at 43 weeks old. The appearance of the male chicken of the Pu'an Panjiang black-bone chicken is as follows: strong body, black feather, black skin, black shank, black comb and black meat. The quality of the semen of the male chicken of the Pu'an Panjiang black-bone chicken is required to be more than 0.5 mL per time, milky white, thick, pH 7-8, density 30-50 billion / mL, viability 0.7, and fertilization rate more than 90%. The standard weight of the female chicken of the Pu'an Panjiang black-bone chicken is 0.45 kg at 8 weeks old, 1.2 kg at 16 weeks old, and the standard egg production at 43 weeks old is more than 65 eggs with pink eggshell, and the average egg weight is 48 g. The appearance of the female chicken of the Pu'an Panjiang black-bone chicken is as follows: medium body, black feather, black skin, black shank, black comb and black meat.

[0023] Further, in step 203, the standard weight of the male chicken of the MP is 1.2 kg at 8 weeks old, 1.9 kg at 16 weeks old, and the appearance of the male chicken of the MP is as follows: large and strong body, purple and black straight single comb, red and yellow comb and a small part of back feather, black wing, abdomen and tail feather, black skin, shank and meat.

[0024] Further, in step 204, the standard weight of the female chicken of the MM is 0.8 kg at 8 weeks old, 1.5 kg at 16 weeks old, and the standard egg production at 43 weeks old is more than 75 eggs with pink eggshell. The appearance of the female chicken of the MM is as follows: medium body, purple and black straight single comb, yellow and black neck feather, chest and part of back feather, black wing, abdomen and tail feather, black skin, shank and meat.

[0025] Further, in step 3), the obtained flower neck green shell Pu'an Panjiang black-bone chicken has the appearance characteristics of "flower neck, black feather, black skin, black shank, black crown, and black meat": the body is relatively large, the body shape is full, the chest is wide and deep, the abdomen is full, the black color is straight and single crown, the overall feather is black with dark green metallic luster, the neck part of the feather is red and yellow, the shank, skin and meat are all black; the obtained flower neck green shell Pu'an Panjiang black-bone chicken cock and hen are put on the market as high-quality broilers at 16 weeks old; the hen is sold as a local characteristic high-quality old hen after the egg production decreases at 43 weeks old.

[0026] Further, the breeding hens in the matching line are subjected to salmonella and chicken leukemia purification treatment before mating.

[0027] The application has the following beneficial effects: the breeding method of the meat and egg dual-purpose flower neck green shell Pu' an Panjiang black-bone chicken complete line introduces the blood of the feathered chicken and Zhuxiang chicken into the blood of the Pu' an Panjiang black-bone chicken, adopts the matching combination ZX x [(MW x PA) x MW], i.e. crossbreeds the male feathered chicken with the female Pu' an Panjiang black-bone chicken to improve the meat performance and egg performance of the offspring under the good hybrid vigor; then uses the female feathered chicken for backcrossing to further improve the meat performance and egg performance of the hybrid offspring; further uses the male Zhuxiang chicken as the terminal paternal parent, the commodity generation inherits the green shell egg gene of the terminal paternal parent, the female chicken produces the greenish egg with excellent egg performance and higher economic benefits, and can be sold as high-quality old female chicken after the egg production; the meat and egg dual-purpose flower neck green shell Pu' an Panjiang black-bone chicken obtained by the application contains 12.5% of the blood of the Pu' an Panjiang black-bone chicken, 50% of the blood of the Zhuxiang chicken, and 37.5% of the blood of the feathered chicken, and has the ideal and unique appearance characteristics of "flower neck, black feather, black skin, black shank, black crown and black meat", the adult chicken has an ideal body shape, complete disease purification and excellent production performance; the application fully utilizes the high-yield performance of the great body weight, early market age, excellent meat performance, high egg production and large egg weight of the great-grandparent generation maternal parent and paternal parent, the feathered chicken MW line, so that the growth rate, market age, 43-week-old egg production and egg weight of the meat and egg dual-purpose flower neck green shell Pu' an Panjiang black-bone chicken are significantly improved, and the market age is effectively shortened; the green shell egg gene of the terminal paternal parent Zhuxiang chicken ZX line and the high production performance of the high egg performance are inherited, so that the commodity generation female chicken produces the greenish shell egg with excellent egg performance and higher economic value. The meat and egg dual-purpose flower neck green shell Pu' an Panjiang black-bone chicken has a large body size, a full body shape, a wide and deep chest, a full abdomen, a black color upright single crown, and black feathers with a greenish metal luster, and the neck part of the feathers is red and yellow, the shank, skin and meat are black, and has the unique appearance characteristics of "flower neck, black feather, black skin, black shank, black crown and black meat"; the male and female chickens have excellent meat performance, and can be marketed as high-quality meat chickens at 16 weeks old, the male chicken has a body weight of more than 2 kg at 16 weeks old, and the female chicken has a body weight of more than 1.4 kg, which is 38 days earlier than the market age of the purebred Pu' an Panjiang black-bone chicken with a market weight at 150 days old, and the growth rate is significantly improved; the female chicken produces the greenish shell egg with higher economic benefits than the powder shell egg before 43 weeks old, can effectively alleviate the imbalance between supply and demand of the green shell egg market, and meet the selection preference of consumers for different shell color eggs; the average egg weight is 52 g, which is significantly higher than that of the purebred Pu' an Panjiang black-bone chicken, and has a significant improvement effect on the egg quality performance such as egg weight and eggshell color. The 43-week-old egg production is 78, which is 13 more than that of the purebred Pu' an Panjiang black-bone chicken, and the egg production performance is significantly improved. Furthermore, the female chicken can be sold as a local characteristic high-quality old female chicken after the 43-week-old egg production decreases, and realizes the meat and egg dual-purpose.Therefore, the meat-egg dual-purpose type flower neck green shell Pu An Panjiang black-bone chicken obtained by the application has the appearance characteristics of flower neck, black feather, black skin, black shank, black crown and black meat, is ideal in appearance, wide in body shape, fast in growth speed, early in market age, high in meat yield, high in production of jade green shell eggs, and dual-purpose in meat and eggs, and is superior to the pure line of the Pu An Panjiang black-bone chicken in body weight, growth speed, slaughtering performance, breeding performance and market age, and the application adopts the individual selection and family selection method to accurately select the meat-egg dual-purpose type flower neck green shell Pu An Panjiang black-bone chicken complete line which is ideal in appearance, wide in body shape, fast in growth speed, early in market age, high in meat yield, high in production of jade green shell eggs and stable in performance. BRIEF DESCRIPTION OF DRAWINGS

[0028] Fig. 1 is a schematic diagram of the breeding method of the meat-egg dual-purpose type flower neck green shell Pu An Panjiang black-bone chicken complete line according to the application;

[0029] Fig. 2 is a picture of appearance characteristics of the breeding of the meat-egg dual-purpose type flower neck green shell Pu An Panjiang black-bone chicken according to the application. DETAILED DESCRIPTION

[0030] As shown in Figs. 1-2 , the breeding method of the meat-egg dual-purpose type flower neck green shell Pu An Panjiang black-bone chicken complete line, comprises the following steps:

[0031] 1) paternal line breeding:

[0032] 101) select the existing Zhuxiang chicken ZX line to meet the 8, 16-week-old body weight standard and the appearance standard, and the female chicken to meet the 8, 16-week-old body weight standard and the 43-week-old egg production standard and the appearance standard, and breed according to the 8, 16-week-old body weight standard and the appearance standard of the male chicken and the 8, 16-week-old body weight standard and the 43-week-old egg production standard and the appearance standard of the female chicken to obtain the great-grandparent ZX paternal father and the great-grandparent ZX paternal mother;

[0033] 102) breed the great-grandparent ZX paternal father and the great-grandparent ZX paternal mother, and breed according to the 8, 16-week-old body weight standard and the appearance standard of the male chicken and the 8, 16-week-old body weight standard and the 43-week-old egg production standard and the appearance standard of the female chicken to obtain the grandparent ZX paternal father and the grandparent ZX paternal mother;

[0034] 103) breed the grandparent ZX paternal father and the grandparent ZX paternal mother, and breed according to the 8, 16-week-old body weight standard, the appearance standard, the 30-week-old male semen quality standard and the green shell egg positive homozygous genotype to obtain the parent ZX paternal father;

[0035] In steps 101) ~ 103), the weight standard of the Zhuxiang cock at 8 weeks of age is 0.7 kg, the weight standard at 16 weeks of age is 1.75 kg, and the egg production standard at 43 weeks of age is more than 80 eggs for the hens of the same family at 43 weeks of age, the appearance characteristics of the Zhuxiang cock are robust physique, solid constitution, feathers with dark green golden luster, well-developed sickle feathers, black feathers, black skin, black shanks, black crown, and black meat, the semen quality requirements of the Zhuxiang cock are more than 0.5 mL of semen collected at a time, milky white, thick, pH 7 ~ 8, density 30 ~ 50 billion / mL, viability 0.7 or more, and fertilization rate 90% or more; the egg production standard of the Zhuxiang hen at 43 weeks of age is more than 80 average eggs, green eggshell color, and average egg weight 50 g, the weight standard of the Zhuxiang hen at 8 weeks of age is 0.65 kg, the weight standard at 16 weeks of age is 1.3 kg, and the appearance characteristics of the Zhuxiang hen are elegant body shape, uniform structure, black feathers, black skin, black shanks, black crown, and black meat. The cock with the positive homozygous genotype of the green-shelled egg is obtained in the following manner: according to the characteristics of the green-shelled egg trait being autosomal dominant inheritance, a population of Zhuxiang chickens is detected by using molecular marker technology, the genotype of the green-shelled egg positive homozygote is selected and reserved, and the ZX line with a green-shelled egg rate of 100% is obtained; wherein the molecular marker technology is realized by using the patent technology with the application number 201510194180.6, the application date of April 22, 2015, and the patent name of primer composition for identifying green-shelled egg chicken genotype and application thereof.

[0036] 2), maternal line selection:

[0037] 201), Select the existing purebred chicken MW line that meets the 8, 16-week-old weight standards and appearance standards of the male chicken and the 8, 16-week-old weight standards and the 43-week-old egg production standards and appearance standards of the female chicken, and according to the 8, 16-week-old weight standards and appearance standards of the male chicken, the inbred chicken MW line and the inbred chicken MW line are obtained. The 8-week-old weight standard of the male chicken is 1.5 kg, the 16-week-old weight standard is 2.4 kg, the 43-week-old egg production standard is that the average egg production of the female chicken of the same family is more than 85, and the appearance standard of the male chicken is as follows: large and strong body, deep red straight single crown, well-developed sickle feather, red and black wings, black skin, shank and meat. The quality of the semen of the male chicken is required to be more than 0.7 mL per time, milky white, thick, pH 7-8, density 30-50 billion / mL, activity 0.7 or more, and fertilization rate 90% or more. The 8-week-old weight standard of the female chicken is 1 kg, the 16-week-old weight standard is 1.6 kg, the 43-week-old egg production standard is more than 85 and the eggshell color is pink, and the average egg weight is 54 g. The appearance standard of the female chicken is large and round, deep red straight single crown, yellow and black skin, shank and meat.

[0038] 202), The existing purebred chicken PA line that meets the 8, 16-week-old weight standards and appearance standards of the male chicken and the 8, 16-week-old weight standards and the 43-week-old egg production standards and appearance standards of the female chicken is mated and selected according to the 43-week-old egg production standards and appearance standards of the female chicken to obtain the great-grandparent PA line. The 8-week-old weight standard of the male chicken is 0.5 kg, the 16-week-old weight standard is 1.4 kg, the 43-week-old egg production standard is that the average egg production of the female chicken of the same family is more than 65, and the appearance standard of the male chicken is as follows: strong body, black feather, black skin, black shank, black crown and black meat. The quality of the semen of the male chicken is required to be more than 0.5 mL per time, milky white, thick, pH 7-8, density 30-50 billion / mL, activity 0.7 or more, and fertilization rate 90% or more. The 8-week-old weight standard of the female chicken is 0.45 kg, the 16-week-old weight standard is 1.2 kg, the 43-week-old egg production standard is more than 65 and the eggshell color is pink, and the average egg weight is 48 g. The appearance standard of the female chicken is medium body, black feather, black skin, black shank, black crown and black meat.

[0039] 203), the great-great-grandparent ZX paternal male and the great-great-grandparent ZX paternal female are bred and selected according to the 8-week-old and 16-week-old weight standards of the male chicken and the appearance standard, to obtain the grandparent MP maternal male; the 8-week-old weight standard of the MP male chicken is 1.2 kg, the 16-week-old weight standard is 1.9 kg, and the appearance standard of the MP male chicken is large and robust, with a purple-black straight single crown, red-yellow comb and a small part of back feathers, black wing, abdomen and tail feathers, black skin, black shank and black meat.

[0040] 204), the grandparent MP maternal male and the grandparent MW maternal female are bred and selected according to the 8-week-old and 16-week-old weight standards of the female chicken and the 43-week-old egg production standard and appearance standard, to obtain the parent generation MM maternal female; the 8-week-old weight standard of the MM female chicken is 0.8 kg, the 16-week-old weight standard is 1.5 kg, the 43-week-old egg production standard is more than 75 eggs, and the eggshell color is pink, and the appearance standard of the MM female chicken is medium size, with a purple-black straight single crown, yellow-brown neck, chest and part of back feathers, black wing, abdomen and tail feathers, black skin, black shank and black meat.

[0041] 3), the parent generation ZX paternal male obtained by paternal selection is mated with the parent generation MM maternal female obtained by maternal selection to obtain the flower-neck green-shell Pu'an Panjiang black chicken. The flower-neck green-shell Pu'an Panjiang black chicken obtained has the appearance characteristics of "flower neck, black feather, black skin, black shank, black crown and black meat": large size, full body, wide and deep chest, full abdomen, black straight single crown, whole black feather with dark green metallic luster, part of neck feather red-yellow, black shank, skin and meat; the male and female chickens are put on the market as high-quality meat chickens at 16 weeks of age; the male chicken weighs more than 2 kg and the female chicken weighs more than 1.4 kg at 16 weeks of age; the female chicken produces emerald green shell eggs before 43 weeks of age, with an average egg weight of 52 g, an egg production of 78 at 43 weeks of age, and is sold as a local characteristic high-quality old hen after the egg production decreases at 43 weeks of age.

[0042] In the above embodiment, in order to ensure the systematization of the breeding of the meat-egg dual-purpose flower-neck green-shell Pu'an Panjiang black chicken, the selection of the great-great-grandparent ZX paternal male, the great-great-grandparent ZX paternal female, the grandparent ZX paternal male, the grandparent ZX paternal female, and the parent generation ZX paternal male in steps 101) to 103) is carried out by single-cage feeding and artificial insemination. Further, the selection of the great-great-grandparent MW maternal male, the great-great-grandparent PA maternal female, the grandparent MP maternal male, the grandparent MW maternal female, and the parent generation MM maternal female in steps 201) to 204) is carried out by single-cage feeding and artificial insemination.

[0043] Further, in order to ensure healthy production of the meat-egg dual-purpose HuaJing green shell PuAnPanjiang black-bone chicken, the breeder hens in the matching system are subjected to Salmonella and chicken leukemia purification treatment before mating.

[0044] The appearance characteristics, growth performance, egg production performance and slaughter performance of the obtained meat-egg dual-purpose HuaJing green shell PuAnPanjiang black-bone chicken are statistically analyzed, the appearance characteristics are shown in Table 1, the growth performance is shown in Table 2, the egg production performance is shown in Table 3, and the slaughter performance is shown in Table 4:

[0045] Table 1 Appearance characteristics

[0046]

[0047] Table 2 Growth performance

[0048]

[0049] Table 3 Egg production performance

[0050]

[0051] Table 4 Slaughter performance

[0052]

[0053] As can be seen from Tables 1 to 4, the meat-egg dual-purpose type flower-neck green-shell Pu' anpanjiang black-bone chicken commodity generation chicken obtained by the application has a larger body shape, a full body shape, a wide and deep chest, a full abdomen, a black color, a single crown, and black feathers with a green metal luster, and part of the neck feathers are red and yellow, and the shank, skin and meat are black, and has the unique appearance characteristics of "flower neck, black feather, black skin, black shank, black crown and black meat"; the cock and hen have excellent meat performance, and can be marketed as high-quality meat chicken at 16 weeks old, and the body weight of the cock is more than 2 kg and the body weight of the hen is more than 1.4 kg at 16 weeks old, which is 38 days earlier than the marketing age of the purebred Pu' anpanjiang black-bone chicken which reaches the marketing weight at 150 days old, and the growth rate is significantly improved; the hen produces higher economic benefits than the green-shell egg with a powder shell before 43 weeks old, and can effectively alleviate the imbalance between supply and demand of the green-shell egg market and meet the selection preference of consumers for different shell color eggs; the average egg weight is 52 g, which is significantly higher than that of the purebred Pu' anpanjiang black-bone chicken, and has a significant improvement effect on egg weight, eggshell color and other egg quality performance. The egg production at 43 weeks old is 78, which is 13 more than that of the purebred Pu' anpanjiang black-bone chicken, and the egg production performance is significantly improved. Furthermore, the hen can be sold as a local characteristic high-quality old hen after the egg production decreases at 43 weeks old, and realizes meat-egg dual-purpose. Therefore, the meat-egg dual-purpose type flower-neck green-shell Pu' anpanjiang black-bone chicken obtained by the application has an ideal body shape and appearance, complete disease purification and excellent production performance; the meat-egg dual-purpose type flower-neck green-shell Pu' anpanjiang black-bone chicken complete line commodity generation has the appearance characteristics of flower neck, black feather, black skin, black shank, black crown and black meat, and has an ideal appearance, a large body shape, a fast growth rate, an early marketing age, a high meat yield, a production of green-shell chicken eggs, meat-egg dual-purpose, and is superior to the pure line of the Pu' anpanjiang black-bone chicken in body weight, growth rate, slaughter performance, reproductive performance and marketing age. Therefore, the application can provide better reference information for the cultivation of the meat-egg dual-purpose type flower-neck green-shell Pu' anpanjiang black-bone chicken complete line.

[0054] The foregoing description shows and describes several preferred embodiments of the application, but it is to be understood that the application is not limited to the foregoing description, should not be construed as excluding other embodiments, and can be used in various other combinations, modifications and environments, and can be modified within the scope of the inventive concept described herein by the above teaching or related technical or knowledge. Any modification and change made by those skilled in the art without departing from the spirit and scope of the application shall be within the protection scope of the appended claims of the application.

Claims

1. A method for breeding a meat and egg dual-purpose type multi-neck green shell Pu' anpanjiang black-bone chicken complete line, comprising the following steps: 1) paternal line breeding: 101) selecting the existing Zhuxiang chicken ZX line male chickens meeting the 8 and 16-week-old weight standards and appearance standards and female chickens meeting the 8 and 16-week-old weight standards and 43-week-old egg production standards and appearance standards for mating and breeding according to the 8 and 16-week-old weight standards and appearance standards of the male chickens and the 8 and 16-week-old weight standards and 43-week-old egg production standards and appearance standards of the female chickens to obtain the great-grandparent ZX paternal sire and the great-grandparent ZX paternal dam; 102) mating the great-grandparent ZX paternal sire and the great-grandparent ZX paternal dam and breeding according to the 8 and 16-week-old weight standards and appearance standards of the male chickens and the 8 and 16-week-old weight standards and 43-week-old egg production standards and appearance standards of the female chickens to obtain the grandparent ZX paternal sire and the grandparent ZX paternal dam; 103) mating the grandparent ZX paternal sire and the grandparent ZX paternal dam and breeding according to the 8 and 16-week-old weight standards, appearance standards, 30-week-old male semen quality standards and green shell egg positive homozygous genotype to obtain the parent ZX paternal sire; 2) maternal line breeding: 201) selecting the existing purebred black-bone chicken MW line male chickens meeting the 8 and 16-week-old weight standards and appearance standards and female chickens meeting the 8 and 16-week-old weight standards and 43-week-old egg production standards and appearance standards for mating and breeding according to the 8 and 16-week-old weight standards and appearance standards of the male chickens to obtain the great-grandparent MW maternal sire and the grandparent MW maternal dam; 202) mating the existing purebred Pu' anpanjiang black-bone chicken PA line male chickens meeting the 8 and 16-week-old weight standards and appearance standards and female chickens meeting the 8 and 16-week-old weight standards and 43-week-old egg production standards and appearance standards and breeding according to the 43-week-old egg production standards and appearance standards of the female chickens to obtain the great-grandparent PA maternal dam; 203) mating the great-grandparent MW maternal sire and the great-grandparent PA maternal dam and breeding according to the 8 and 16-week-old weight standards and appearance standards of the male chickens to obtain the grandparent MP maternal sire; 204) backcross mating the grandparent MP maternal sire and the grandparent MW maternal dam and breeding according to the 8 and 16-week-old weight standards and 43-week-old egg production standards and appearance standards of the female chickens to obtain the parent MM maternal dam; 3) mating the parent ZX paternal sire obtained by paternal line breeding with the parent MM maternal dam obtained by maternal line breeding to obtain the multi-neck green shell Pu' anpanjiang black-bone chicken.

2. The method for breeding of the meat-and-egg type Huajing green-shell Pu' anpanjiang black-bone chicken complete line according to claim 1, characterized in that: In steps 101) to 103), the breeding of the great-grandparent ZX paternal sire, the great-grandparent ZX paternal dam, the grandparent ZX paternal sire, the grandparent ZX paternal dam and the parent ZX paternal sire is carried out by single-cage feeding and artificial insemination.

3. The method for breeding of the meat-and-egg type Huajing green-shell Pu' anpanjiang black-bone chicken complete line according to claim 1, characterized in that: In steps 201) to 204), the breeding of the great-grandparent MW maternal sire, the great-grandparent PA maternal dam, the grandparent MP maternal sire, the grandparent MW maternal dam and the parent MM maternal dam is carried out by single-cage feeding and artificial insemination.

4. The method for breeding of the meat and egg type Huajing green shell Pu' an Panjiang black-bone chicken complete line according to claim 1, characterized in that: In steps 101)~103), the weight standard of the Zhuxiang cock at 8 weeks old is 0.7 kg, the weight standard at 16 weeks old is 1.75 kg, and the egg production standard of the Zhuxiang hen at 43 weeks old is more than 80 eggs, the appearance characteristics of the Zhuxiang cock are robust physique, solid constitution, ink green golden luster of feathers, well-developed sickle feather, black feathers, black skin, black shank, black comb and black meat, the semen quality requirements of the Zhuxiang cock are more than 0.5 mL of semen collected at a time, milky white, thick, pH 7~8, density 30~50 billion / mL, viability 0.7 and fertilization rate more than 90%, the egg production standard of the Zhuxiang hen at 43 weeks old is more than 80 eggs, the average egg weight is 50 g, and the eggshell color is green, the weight standard of the Zhuxiang hen at 8 weeks old is 0.65 kg, the weight standard at 16 weeks old is 1.3 kg, and the appearance characteristics of the Zhuxiang hen are slim body, uniform structure, black feathers, black skin, black shank, black comb and black meat.

5. The method for breeding of the meat-and-egg type Huajing green-shell Pu' anpanjiang black-bone chicken complete line according to claim 1, characterized in that: In step 201), the weight standard of the Ma-feather black chicken cock at 8 weeks old is 1.5 kg, the weight standard at 16 weeks old is 2.4 kg, and the egg production standard of the Ma-feather black chicken hen at 43 weeks old is more than 85 eggs, the appearance standard of the Ma-feather black chicken cock is as follows: robust physique, deep red straight single comb, well-developed sickle feather, red and black feathers, black skin, black shank and black meat, the semen quality requirements of the Ma-feather black chicken cock are more than 0.7 mL of semen collected at a time, milky white, thick, pH 7~8, density 30~50 billion / mL, viability 0.7 and fertilization rate more than 90%, the weight standard of the Ma-feather black chicken hen at 8 weeks old is 1 kg, the weight standard at 16 weeks old is 1.6 kg, the egg production standard at 43 weeks old is more than 85 eggs, the eggshell color is pink, and the average egg weight is 54 g, and the appearance standard of the Ma-feather black chicken hen is as follows: wide and round body, deep red straight single comb, yellow and black feathers, black skin, black shank and black meat.

6. The method for breeding of the meat-and-egg type Huajing green-shell Pu' anpanjiang black-bone chicken complete line according to claim 1, characterized in that: In step 202), the weight standard of the Pu'an Panjiang black chicken cock at 8 weeks old is 0.5 kg, the weight standard at 16 weeks old is 1.4 kg, and the egg production standard of the Pu'an Panjiang black chicken hen at 43 weeks old is more than 65 eggs, the appearance standard of the Pu'an Panjiang black chicken cock is robust physique, black feathers, black skin, black shank, black comb and black meat, the semen quality requirements of the Pu'an Panjiang black chicken cock are more than 0.5 mL of semen collected at a time, milky white, thick, pH 7~8, density 30~50 billion / mL, viability 0.7 and fertilization rate more than 90%, the weight standard of the Pu'an Panjiang black chicken hen at 8 weeks old is 0.45 kg, the weight standard at 16 weeks old is 1.2 kg, the egg production standard at 43 weeks old is more than 65 eggs, the eggshell color is pink, and the average egg weight is 48 g, and the appearance standard of the Pu'an Panjiang black chicken hen is as follows: medium body, black feathers, black skin, black shank, black comb and black meat.

7. The method for breeding of the meat-and-egg type Huajing green-shell Pu' anpanjiang black-bone chicken complete line according to claim 1, characterized in that: In step 203), the MP cock is 8 weeks old, the weight standard is 1.2 kg, the weight standard is 1.9 kg at 16 weeks old, the appearance standard of the MP cock is large and strong, purple black straight single crown, red yellow comb and a small part of back feathers, black wing, abdomen and tail feathers, black skin, black shank and black meat.

8. The method for breeding of the meat-and-egg type Huajing green-shell Pu' anpanjiang black-bone chicken complete line according to claim 1, characterized in that: In step 204), the MM hen is 8 weeks old, the weight standard is 0.8 kg, the weight standard is 1.5 kg at 16 weeks old, and the egg production standard is more than 75 eggs at 43 weeks old, and the eggshell color is pink, the appearance standard of the MM hen is medium size, purple black straight single crown, neck feather, chest and part of back feather are yellowish brown, black wing, abdomen and tail feather, black skin, black shank and black meat.

9. The method for breeding of the meat-and-egg type Huajing green-shell Pu' anpanjiang black-bone chicken complete line according to claim 1, characterized in that: In step 3), the obtained flower neck green shell Pu'anpanjiang black-bone chicken has the appearance characteristics of "flower neck, black feather, black skin, black shank, black crown and black meat": large body size, full body shape, wide and deep chest, full abdomen, black straight single crown, whole feather is black with dark green metal luster, part of neck feather is red yellow, shank, skin and meat are black; the obtained flower neck green shell Pu'anpanjiang black-bone chicken cock and hen are put on the market as high-quality meat chicken at 16 weeks old; the hen is sold as a local characteristic high-quality old hen after the egg production decreases at 43 weeks old.

10. The method for breeding of the meat-and-egg type Huajing green-shell Pu' anpanjiang black-bone chicken complete line according to claim 1, characterized in that: The breeding chickens in the matched line are treated for salmonella and chicken leukemia purification before mating.

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