Composite coarse cereal meal replacement powder and preparation technology thereof

Through enzymatic decomposition, microwave extraction and gradient fermentation, composite grain meal replacement powder is prepared, which solves the problem of nutrient destruction of traditional meal replacement powder in high-temperature treatment, and achieves better blood lipid-lowering effect and storage stability.

CN119924513APending Publication Date: 2025-05-06ZHENGZHOU UNIV
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Patent Information

Application Number
CN202411877883.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-19
Publication Date
2025-05-06

AI Technical Summary

Technical Problem

The existing medicinal meal replacement powder has severely damaged the nutritional components during the high temperature treatment process, affecting the taste and nutritional value.

Method used

The preparation process of compound mixed grain meal replacement powder is adopted, and the active ingredients are extracted through enzymatic lysis and microwave extraction technology, and the effective ingredients such as total sugar, polyphenols and total flavonoids are retained through gradient fermentation and refining treatment.

Benefits of technology

It improves the blood lipid-lowering effect of meal replacement powder, improves storage stability, and ensures the retention and performance of effective ingredients.

✦ Generated by Eureka AI based on patent content.
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Abstract

The invention belongs to the technical field of food, and particularly relates to composite coarse cereal meal replacement powder and a preparation technology thereof. The composite coarse cereal meal replacement powder comprises the following raw materials in parts by mass: 12-16 parts of radix puerariae powder, 14-19 parts of poria cocos, 14-18 parts of oat, 11-14 parts of black beans, 5-10 parts of peas, 30-36 parts of black rice, 10-14 parts of tartary buckwheat, 3-7 parts of rhizoma polygonati, 6-10 parts of Chinese wolfberry fruits, 1-4 parts of heartleaf houttuynia herbs, 3-7 parts of honeysuckle flowers, 1-4 parts of mint, 0.5-1.5 parts of wrinkled gianthyssop herbs and 2-5 parts of licorice roots. The meal replacement powder prepared by a special preparation process has a satiety effect and also has the functions of reducing fat, improving immunity, soothing the nerves, stabilizing the mind and nourishing the stomach.
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Description

Technical Field

[0001] The invention belongs to the technical field of food, and particularly relates to a composite grain meal replacement powder and a preparation process thereof. Background Art

[0002] At present, the dietary structure of Chinese people is not reasonable, and the proportion of fat intake is high, which leads to obesity, tumors, cardiovascular diseases and other problems. Weight loss meal replacement foods have the characteristics of high fiber, low calories, and easy to fill the stomach. Therefore, eating meal replacements can effectively control food intake and calories in food, thereby achieving the purpose of weight loss. It has been loved by people who want to lose weight all over the world.

[0003] Medicinal meal replacement powder is a meal replacement product that combines traditional Chinese medicine ingredients and nutrients. It aims to provide users with healthy and convenient dietary choices. This type of product usually contains a variety of natural ingredients such as traditional Chinese medicines and grains. Through scientific proportions, it can not only meet daily nutritional needs, but also give full play to the health benefits of traditional Chinese medicines. Traditional medicinal meal replacement powders are mostly made by decoction, with simple processing methods. Long-term high-temperature treatment causes serious damage to the nutrients in the medicinal raw materials, which has many adverse effects on taste and nutrition.

[0004] Therefore, there is an urgent need for a composite grain meal replacement powder and a preparation process thereof. Summary of the invention

[0005] The purpose of the present invention is to provide a composite multi-grain meal replacement powder and a preparation process thereof.

[0006] In order to achieve the above object, the present invention provides the following technical solutions: A composite grain meal replacement powder comprises the following raw materials in parts by weight: 12-16 parts of kudzu root powder, 14-19 parts of poria cocos, 14-18 parts of oats, 11-14 parts of black beans, 5-10 parts of peas, 30-36 parts of black rice, 10-14 parts of bitter buckwheat powder, 3-7 parts of polygonatum, 6-10 parts of wolfberry, 1-4 parts of houttuynia cordata, 3-7 parts of honeysuckle, 1-4 parts of mint, 0.5-1.5 parts of patchouli, and 2-5 parts of liquorice.

[0007] Pueraria root: It mainly contains isoflavones (puerarin, daidzein), triterpenes (pueraria saponin A, pueraria saponin B methyl ester), coumarins (coumarinol, pueraria phenol), alkaloids (5-methylhydantoin, allantoin), carbohydrates (pueraria polysaccharide), amino acids, minerals (K, Zn, Fe) and other ingredients. It has the effects of lowering blood lipids, blood pressure, blood sugar, protecting against brain damage, protecting the liver, sobering up, lowering uric acid, anti-oxidation, anti-aging, and enhancing immunity.

[0008] Poria: It mainly contains carbohydrates (Poria polysaccharides), triterpenes (Poria acid, Poria acid A), vitamins, proteins, choline and other ingredients. It has antioxidant, hypoglycemic, immune-enhancing, anti-tumor, kidney protection, anti-inflammatory, liver protection, diuretic, anti-aging and whitening effects.

[0009] Polygonatum: It mainly contains carbohydrates (polysaccharides, oligosaccharides), saponins (diosgenin, diosgenin), lignin, flavonoids, alkaloids, quinones, amino acids and other ingredients. It has the effects of lowering blood lipids, lowering blood sugar, anti-aging, anti-tumor, regulating immunity, and improving memory.

[0010] Lycium barbarum: It mainly contains carbohydrates (lycium barbarum polysaccharides), carotenoids (carotenoids, carotenoid esters), alkaloids (betaine, melatonin), polyphenols (total flavonoids, protocatechuic acid), amino acids, minerals (Mn, Cu, Zn) and other ingredients. It has the effects of lowering blood sugar, anti-oxidation, protecting the liver, anti-tumor, regulating immunity, anti-aging, protecting nerves, and protecting vision.

[0011] Oats: Mainly contain dietary fiber (β-glucan), flavonoids (quercetin, kaempferol), phenolic acids (ferulic acid and its derivatives), saponins, alkaloids, protein, fat and other ingredients. It has the functions of nutrition, regulating blood lipids, lowering blood sugar, lowering cholesterol, regulating insulin levels, improving large intestine function, anti-oxidation, antibacterial, anti-inflammatory and antipruritic, anti-tumor, anti-caries, improving immunity, regulating sports fatigue or skeletal muscle metabolism, improving blood microcirculation, and enhancing memory and thinking ability.

[0012] Black beans: mainly contain flavonoids (flavonoids, isoflavones, flavonols, dihydroflavonoids, flavanones, etc.), proteins (salt-soluble globulins, antibacterial proteins, chitinase, SOD, POD, anti-nutritional factors), red pigments (anthocyanins), saponins (pentacyclic triterpenoid oleanolic acid total glycosides), distilled oil, polysaccharides, fatty acids and other ingredients. It has nutritional, antioxidant, anti-tumor, prevention of cardiovascular diseases, antiviral, anti-inflammatory, antibacterial, blood sugar lowering, vision improvement, prevention of arteriosclerosis, anti-HIV, liver protection, antipruritic and anti-inflammatory, treatment of skin diseases, and immunity regulation.

[0013] Pea: Mainly contains pea protein, pea polypeptide, pea starch, dietary fiber, trypsin inhibitors (13 subtypes, mainly A1 and A2 subunits), phenols (flavonoids, phenolic acids, tannins and anthocyanins and their derivatives) and other ingredients. It has the effects of enhancing human immunity, preventing cardiovascular and cerebrovascular diseases, removing excess free radicals from the human body, preventing cancer, delaying aging, lowering blood sugar, lowering cholesterol, improving colon function, preventing colon cancer, cardiovascular disease, diabetes, anti-inflammatory, anti-radiation, anti-disease and anti-virus.

[0014] Black rice: It mainly contains anthocyanins, flavonoids, phenolic acids (protocatechuic acid, ferulic acid, vanillic acid), γ-aminobutyric acid, fats (unsaturated fatty acids, oleic acid and linoleic acid), dietary fiber and other ingredients. It has nutritional, antioxidant, lipid-lowering, blood pressure-lowering, blood sugar regulating, liver protection, nerve protection, anti-tumor, promotion of the proliferation of probiotic flora, antibacterial, anti-allergic, anti-asthma, anti-inflammatory and hemostatic, immunity-enhancing, mood adjustment, sleep improvement, brain strengthening, liver and kidney protection, and weight loss effects.

[0015] Buckwheat: It mainly contains buckwheat oil (oleic acid, linoleic acid, carotenoids, tocopherol), protein / peptide (albumin, glutenin, alcohol-soluble protein, globulin, lysine), flavonoids (rutin, quercetin), starch, polysaccharides, phenolic acids (protocatechuic acid, ferulic acid, parahydroxybenzoic acid) and other ingredients. It has antioxidant, prevention and treatment of lipid metabolism disorders, protection of the liver and atherosclerosis, anti-aging, anti-fatigue, lowering blood sugar, improving constipation, inhibiting colorectal cancer and gallstones, anti-edema, antibacterial, anti-tumor, lowering blood lipids, anti-inflammatory and antipruritic, and promoting brain protein kinase activity. It can be used as a high-quality source of dietary tocopherol.

[0016] The present invention provides a preparation process of a composite grain meal replacement powder, comprising the following steps: (1) Drying polygonatum, wolfberry and houttuynia cordata separately and then crushing them into less than 20 mesh, mixing them to obtain a first mixed material, adding water to the first mixed material to obtain a first mixed material liquid, adding a composite enzyme, performing enzymolysis at 30-35° C. for 4-7 hours, inactivating the enzyme, obtaining an enzymolysis product, placing the enzymolysis product in a microwave reactor, with a microwave power of 200-250 W, a temperature of 70-75° C., and an extraction time of 1.5-2 hours to obtain a first extract, filtering the extract using a 200-300 mesh filter, and filtering to obtain a first filtrate; (2) The honeysuckle, mint, patchouli and liquorice are dried and crushed to less than 20 meshes respectively, mixed, and then extruded through a screw extruder to obtain a second mixed material; water is added to the second mixed material to obtain a second mixed material liquid, Bifidobacterium longum is added, and anaerobically fermented at 35-38°C for 10-12 hours. After sterilization, Bifidobacterium lactis is continuously added, and anaerobically fermented at 35-38°C for 5-8 hours. After sterilization, Lactobacillus rhamnosus is continuously added, and anaerobically fermented at 35-38°C for 15-17 hours. After sterilization, a second extract is obtained, which is filtered using a 200-300 mesh filter to obtain a second filtrate; (3) mixing the first filtrate and the second filtrate, adding poria cocos, oats, black beans, peas, black rice, and bitter buckwheat, and soaking them to obtain a soaking mixture; (4) adding kudzu root powder to the soaked mixture and grinding the mixture, and passing the ground mixture through a 60-80 mesh sieve to obtain a ground mixture; (5) heating the pulped mixture at 70-75° C. with stirring for 30-40 minutes to obtain a cooked material; (6) The cooked material is subjected to a low-temperature freeze-drying treatment to obtain a dry material, the moisture content of the material is controlled to be 4-6%, the material is crushed, and passed through a 200-mesh sieve to obtain a composite grain meal replacement powder.

[0017] The present invention selects a variety of raw materials for preparing meal replacement powder. The meal replacement powder prepared by the traditional water extraction method has poor lipid-lowering effect and storage stability. The present invention divides the raw materials into four groups by designing a reasonable process, and adds them at different stages. The active components in the prepared meal replacement powder can be extracted to the greatest extent, thereby improving the lipid-lowering effect of the meal replacement powder. At the same time, the active components in the meal replacement powder have antioxidant and antiseptic effects, and the storage stability is improved.

[0018] Furthermore, the mass of water in step (1) is 8-10 times the mass of the first mixture.

[0019] Furthermore, the complex enzyme in step (1) includes cellulase, bromelain and pectinase.

[0020] Furthermore, in the step (1), the enzyme dosage of cellulase in the first mixed liquid is 100-120 U / mL; the enzyme dosage of bromelain in the first mixed liquid is 70-90 U / mL; and the enzyme dosage of pectinase in the first mixed liquid is 130-150 U / mL.

[0021] The present invention first uses enzymatic hydrolysis and then microwave extraction on polygonatum, wolfberry and houttuynia cordata, first performs heat explosion treatment on honeysuckle, mint, patchouli and licorice and then uses specific fungi to perform gradient fermentation, so that the total sugar, polyphenols and total flavonoids in the raw materials can be extracted to the maximum extent, thereby improving the blood lipid lowering effect of the meal replacement powder.

[0022] Furthermore, in step (2), the four-stage temperature gradient of the screw heating is 70-80°C, 100-110°C, 130-140°C, and 160-170°C.

[0023] Furthermore, the mass of water in step (2) is 8-10 times the mass of the second mixture.

[0024] Furthermore, the amount of Bifidobacterium longum in the second mixed liquid is 10 6 -10 7 CFU / mL; the amount of Bifidobacterium lactis in the second mixed liquid is 10 10 -10 11 CFU / mL; the amount of Lactobacillus rhamnosus in the second mixed liquid is 10 8 -10 9 CFU / mL.

[0025] Furthermore, in step (3), the immersion treatment is carried out at 40-50° C. for 6-10 hours.

[0026] Furthermore, the ratio of the total mass of polygonatum, wolfberry, and houttuynia cordata to the total mass of honeysuckle, mint, patchouli, and licorice is (15-17): (12-14).

[0027] During the experiment, it was found that under the preparation process of the present invention, the relationship between the total mass of polygonatum, wolfberry, houttuynia cordata and the total mass of honeysuckle, mint, patchouli, and licorice can affect the lipid-lowering effect of the meal replacement powder. The analysis shows that the active components in different raw materials are different. Under this ratio, more total sugars, polyphenols and total flavonoids can be obtained. At the same time, when the two groups of extraction products are mixed, the active components contained in each other will affect each other and produce a synergistic effect. Under this ratio condition, the lipid-lowering effect can be maximized.

[0028] Compared with the prior art, the advantages and beneficial effects of the present invention are: 1. The present invention adopts a multi-path step-by-step addition preparation process, adopts different processing paths according to the characteristics of different materials, and uses a specific preparation method to fully retain the effective ingredients such as total sugar, polyphenols and total flavonoids in the raw materials, while removing non-effective ingredients, thereby achieving the enrichment of effective ingredients and ensuring the health care effect of the meal replacement powder.

[0029] 2. The present invention divides the raw materials into four groups by designing a reasonable process, and adds them at different stages, so that the active components in the prepared meal replacement powder can be extracted to the greatest extent, thereby improving the lipid-lowering effect of the meal replacement powder. At the same time, the active components in the meal replacement powder have antioxidant and antiseptic effects, and the storage stability is improved.

[0030] 3. The preparation process of the present invention is closely related to the raw material ratio, which produces a synergistic effect. Under the preparation process of the present invention, the total mass of polygonatum, wolfberry, and houttuynia cordata and the total mass of honeysuckle, mint, patchouli, and licorice are in a specific ratio, which can improve the lipid-lowering effect of the meal replacement powder. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] Bifidobacterium longum, accession number: SHBCCD24310, was purchased from Shanghai Microbiological Collection Center.

[0033] Bifidobacterium lactis, accession number: SHBCCD24728, was purchased from Shanghai Microbiological Collection Center.

[0034] Lactobacillus rhamnosus, accession number: SHBCCD73158, was purchased from Shanghai Microbiological Collection Center.

[0035] Lactobacillus bulgaricus, accession number: CCTCC CB 20082295, was purchased from China Center for Type Culture Collection.

[0036] Lactobacillus helveticus, accession number: CCTCC AB 2010205, was purchased from China Center for Type Culture Collection.

[0037] Lactobacillus brevis, accession number: SHBCC D14346, was purchased from Shanghai Microbiological Collection Center.

[0038] The enzymes used in the present invention were purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0039] Pueraria root powder was purchased from Guangdong Mingtong Biotechnology Co., Ltd.

[0040] Example 1 The present embodiment provides a composite grain meal replacement powder, comprising the following raw materials in parts by weight: 14 parts of kudzu root powder, 15 parts of poria cocos, 17 parts of oats, 12 parts of black beans, 6 parts of peas, 32 parts of black rice, 13 parts of bitter buckwheat powder, 5 parts of polygonatum, 8 parts of wolfberry, 2 parts of houttuynia cordata, 5 parts of honeysuckle, 3 parts of mint, 1 part of patchouli, and 4 parts of licorice.

[0041] The preparation process of the composite grain meal replacement powder comprises the following steps: (1) Polygonatum sibiricum, wolfberry and houttuynia cordata are dried and crushed to less than 20 meshes respectively, and mixed to obtain a first mixture. Water is added to the first mixture, and the mass of the water is 9 times the mass of the first mixture to obtain a first mixture liquid. A composite enzyme is added, and the composite enzyme includes cellulase, bromelain and pectinase. The enzyme amount of cellulase in the first mixture liquid is 110U / mL of the first mixture liquid; the enzyme amount of bromelain in the first mixture liquid is 80U / mL of the first mixture liquid; the enzyme amount of pectinase in the first mixture liquid is 140U / mL of the first mixture liquid. Enzyme hydrolysis is performed at 32°C for 6h, and after enzyme inactivation, an enzymolysis product is obtained. The enzymolysis product is placed in a microwave reactor with a microwave power of 230W, a temperature of 72°C, and an extraction time of 1.8h to obtain a first extract, which is filtered using a 200-mesh filter to obtain a first filtrate. (2) Honeysuckle, mint, patchouli, and liquorice are dried and crushed to less than 20 meshes, mixed, and then passed through a screw extruder for screw heating. The four-stage temperature gradient of the screw heating is 75°C, 105°C, 135°C, and 165°C. The second mixed material is extruded; water is added to the second mixed material, the mass of the water is 9 times the mass of the second mixed material, to obtain a second mixed material liquid, and Bifidobacterium longum is added. The amount of Bifidobacterium longum in the second mixed material liquid is 10 7 CFU / mL second mixed liquid; anaerobic fermentation at 37°C for 11h, after sterilization, continue to add Bifidobacterium lactis, the amount of Bifidobacterium lactis in the second mixed liquid is 10 11 CFU / mL second mixed liquid; anaerobic fermentation at 36°C for 7h, after sterilization, continue to add Lactobacillus rhamnosus, the amount of Lactobacillus rhamnosus in the second mixed liquid is 10 8 CFU / mL second mixed liquid, anaerobic fermentation at 36°C for 16h, sterilization, to obtain a second extract, filtration treatment, the filter screen used is 200 mesh, after filtration to obtain a second filtrate; (3) mixing the first filtrate and the second filtrate, adding Poria cocos, oats, black beans, peas, black rice, and bitter buckwheat, and soaking at 45° C. for 8 h to obtain a soaking mixture; (4) adding kudzu root powder to the soaked mixture for grinding, and passing the ground mixture through a 60-mesh sieve to obtain a ground mixture; (5) heating the refined mixture at 72° C. with stirring for 35 minutes to obtain a cooked material; (6) The cooked material is subjected to a low-temperature freeze-drying treatment to obtain a dry material, the moisture content of the material is controlled to 5%, the material is crushed, and passed through a 200-mesh sieve to obtain a composite grain meal replacement powder.

[0042] Example 2 The present embodiment provides a composite multi-grain meal replacement powder, comprising the following raw materials in parts by weight: 12 parts of kudzu root powder, 19 parts of poria cocos, 14 parts of oats, 14 parts of black beans, 5 parts of peas, 36 parts of black rice, 10 parts of tartary buckwheat powder, 7 parts of polygonatum, 6 parts of wolfberry, 4 parts of houttuynia cordata, 3 parts of honeysuckle, 4 parts of mint, 0.5 part of patchouli, and 5 parts of licorice.

[0043] The preparation process of the composite grain meal replacement powder comprises the following steps: (1) Polygonatum sibiricum, wolfberry and houttuynia cordata are dried and crushed to less than 20 meshes respectively, and mixed to obtain a first mixture. Water is added to the first mixture, and the mass of the water is 8 times the mass of the first mixture to obtain a first mixed liquid. A composite enzyme is added, and the composite enzyme includes cellulase, bromelain and pectinase. The enzyme amount of cellulase in the first mixed liquid is 120 U / mL; the enzyme amount of bromelain in the first mixed liquid is 70 U / mL; the enzyme amount of pectin in the first mixed liquid is 150 U / mL. Enzyme hydrolysis is performed at 30° C. for 7 h. After the enzyme is inactivated, an enzymatic hydrolysis product is obtained. The enzymatic hydrolysis product is placed in a microwave reactor with a microwave power of 200 W, a temperature of 75° C., and an extraction time of 2 h to obtain a first extract. The first extract is filtered using a 200-mesh filter to obtain a first filtrate. (2) Honeysuckle, mint, patchouli, and liquorice are dried and crushed to less than 20 meshes, mixed, and then passed through a screw extruder for screw heating. The four-stage temperature gradient of the screw heating is 80°C, 100°C, 140°C, and 160°C. The second mixed material is extruded to obtain a second mixed material; water is added to the second mixed material, the mass of the water is 10 times the mass of the second mixed material, to obtain a second mixed material liquid, and Bifidobacterium longum is added. The amount of Bifidobacterium longum in the second mixed material liquid is 10 6 CFU / mL; anaerobic fermentation at 38°C for 10 h, after sterilization, continue to add Bifidobacterium lactis, the amount of Bifidobacterium lactis in the second mixed liquid is 10 10 CFU / mL; 38℃ anaerobic fermentation for 5h, after sterilization, continue to add Lactobacillus rhamnosus, the amount of Lactobacillus rhamnosus in the second mixed liquid is 10 9 CFU / mL, anaerobic fermentation at 35°C for 17h, sterilization, to obtain the second extract, filtration treatment, the filter screen used is 200 mesh, and the second filtrate is obtained after filtration; (3) mixing the first filtrate and the second filtrate, adding Poria cocos, oats, black beans, peas, black rice, and bitter buckwheat, and soaking at 50° C. for 6 h to obtain a soaking mixture; (4) adding kudzu root powder to the soaked mixture for grinding, and passing the ground mixture through an 80-mesh sieve to obtain a ground mixture; (5) heating the pulped mixture at 70° C. with stirring for 40 min to obtain a cooked material; (6) The cooked material is subjected to a low-temperature freeze-drying treatment to obtain a dry material, the moisture content of the material is controlled to 4%, the material is crushed, and passed through a 200-mesh sieve to obtain a composite grain meal replacement powder.

[0044] Comparative Example 1 The difference between this comparative example and Example 1 is: a composite grain meal replacement powder, comprising the following raw materials in parts by weight: 32 parts of kudzu root powder, 6 parts of poria cocos, 12 parts of oats, 13 parts of black beans, 15 parts of peas, 24 parts of black rice, 17 parts of buckwheat powder, 8 parts of polygonatum, 5 parts of wolfberry, 5 parts of houttuynia cordata, 2 parts of honeysuckle, 1 part of mint, 3 parts of patchouli, and 4 parts of licorice.

[0045] Comparative Example 2 The difference between this comparative example and Example 1 is: a composite grain meal replacement powder, comprising the following raw materials in parts by weight: 14 parts of kudzu root powder, 15 parts of poria cocos, 17 parts of oats, 12 parts of black beans, 6 parts of peas, 32 parts of black rice, 13 parts of bitter buckwheat powder, 3 parts of polygonatum, 6 parts of wolfberry, 1 part of houttuynia cordata, 7 parts of honeysuckle, 4 parts of mint, 2 parts of patchouli, and 5 parts of licorice.

[0046] Comparative Example 3 The difference between this comparative example and Example 1 is that the complex enzyme includes neutral protease, ficin and β-glucanase.

[0047] Comparative Example 4 The difference between this comparative example and Example 1 is that the enzyme dosage of cellulase in the first mixed liquid is 140 U / mL; the enzyme dosage of bromelain in the first mixed liquid is 110 U / mL; and the enzyme dosage of pectinase in the first mixed liquid is 80 U / mL.

[0048] Comparative Example 5 The difference between this comparative example and Example 1 is that no screw heating is performed.

[0049] Honeysuckle, mint, patchouli, and liquorice were dried and crushed to less than 20 meshes, and mixed to obtain a second mixed material; water was added to the second mixed material, the mass of the water being 9 times the mass of the second mixed material, to obtain a second mixed material liquid, and Bifidobacterium longum was added, and the amount of Bifidobacterium longum in the second mixed material liquid was 10 7 CFU / mL; anaerobic fermentation at 37°C for 11h, after sterilization, continue to add Bifidobacterium lactis, the amount of Bifidobacterium lactis in the second mixed liquid is 10 11 CFU / mL; 36℃ anaerobic fermentation for 7h, after sterilization, continue to add Lactobacillus rhamnosus, the amount of Lactobacillus rhamnosus in the second mixed liquid is 10 8 CFU / mL, anaerobic fermentation at 36°C for 16h, sterilization, to obtain the second extract, filtration treatment, the filter screen used is 200 mesh, after filtration to obtain the second filtrate.

[0050] Comparative Example 6 The difference between this comparative example and Example 1 is that Bifidobacterium longum is replaced by Lactobacillus bulgaricus, Bifidobacterium lactis is replaced by Lactobacillus helveticus, and Lactobacillus rhamnosus is replaced by Lactobacillus brevis.

[0051] Comparative Example 7 The difference between this comparative example and Example 1 is that the preparation process of the composite grain meal replacement powder comprises the following steps: (1) Dry polygonatum, wolfberry, houttuynia cordata, honeysuckle, mint, patchouli, and liquorice, respectively, and grind them into pieces smaller than 20 meshes, mix them, add 10 times the mass of water, heat until the water boils, and boil for 1 hour; filter the mixture using a 200-mesh filter to obtain a filtrate; (2) adding Poria cocos, oats, black beans, peas, black rice, and bitter buckwheat to the filtrate, and soaking the mixture at 45° C. for 8 h to obtain a soaking mixture; (3) adding kudzu root powder to the soaked mixture for grinding, and passing the ground mixture through a 60-mesh sieve to obtain a ground mixture; (4) heating the pulped mixture at 72° C. with stirring for 35 minutes to obtain a cooked material; (5) The cooked material is subjected to a low-temperature freeze-drying treatment to obtain a dry material, the moisture content of the material is controlled to 5%, the material is crushed, and passed through a 200-mesh sieve to obtain a composite grain meal replacement powder.

[0052] Performance Testing 1. Blood lipid lowering experiment Experimental animals: 4-week-old healthy male rats, fed with basic feed for 6 days; high-fat feed formula: 20% lard, 1% cholesterol, 0.2% bile salt, 18% sugar, and the rest high-nutrition basic feed.

[0053] Experimental method: Rats were evenly divided into 9 groups according to body weight, with 10 rats in each group, of which 1 group was used as a blank experimental control group and was fed with basic feed and normal saline. The remaining groups were re-merged to model hyperlipidemia, and were fed with high-fat feed and normal saline. During this period, blood lipid indexes were tested until the modeling was successful. The rats after successful modeling were re-grouped according to the results of blood lipid testing, with 10 rats in each group, so that the blood lipid levels of each group were basically the same. During the modeling period, the blank control group was fed with basic feed and normal saline normally; the modeling time was about three weeks. After the modeling was completed, the experimental group was fed with the meal replacement powder and normal saline of Examples 1-2 and Comparative Examples 1-7, respectively, and the positive control group was fed with basic feed and normal saline.

[0054] The feeding amount of basic feed and meal replacement powder was 50 mg / kg, and the feeding method was gavage. After the experimental group started the experiment, the blood lipid content was tested after the experimental group samples were fed continuously for 4 weeks.

[0055] Blood collection and testing methods: Intraperitoneal blood sampling method, to test the content of total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL-C) and low-density lipoprotein (LDL-C), blood lipid index test results unit (mmol / L).

[0056] After 4 weeks of continuous feeding, blood lipid tests were performed.

[0057] The results are shown in Table 1.

[0058] Table 1 Blood lipid test results (unit: mmol / L) test Total cholesterol Triglycerides High-density lipoprotein Low-density lipoprotein Blank control 4.67±0.3 1.59±0.3 2.35±0.3 0.81±0.1 Example 1 35.6±2.7 5.5±2.3 12.8±0.9 12.4±3.1 Example 2 35.9±2.4 5.8±2.6 12.9±0.7 12.9±3.5 Comparative Example 1 43.6±2.7 7.6±2.5 8.2±1.1 18.4±3.1 Comparative Example 2 38.5±2.8 6.1±2.3 10.2±0.5 15.6±3.8 Comparative Example 3 40.2±2.1 8.1±2.1 9.8±1.5 16.9±2.6 Comparative Example 4 40.8±2.4 8.3±2.2 9.6±1.2 17.2±2.5 Comparative Example 5 41.2±2.0 8.6±1.7 9.4±0.8 17.5±2.9 Comparative Example 6 41.7±2.3 8.9±1.8 9.2±0.9 17.8±3.1 Comparative Example 7 43.2±2.6 7.3±2.0 8.7±1.4 18.0±3.2 Positive Control 49.1±6.1 10.3±3.8 12.6±1.2 21.9±3.7 It can be seen from the results that the total cholesterol, triglyceride and low-density lipoprotein contents in Examples 1-2 decreased significantly, while the high-density lipoprotein content, which is beneficial to blood lipid regulation, was higher than that in the positive control group, indicating that the meal replacement powders of Examples 1-2 have a significant lipid-lowering effect. Comparative Examples 1-7 change the formula of raw materials and the preparation process, and the lipid-lowering effects are all reduced to varying degrees. It should be noted that Comparative Example 2 changes the ratio of the total mass of polygonatum, wolfberry, and houttuynia cordata to the total mass of honeysuckle, mint, patchouli, and licorice, resulting in a decrease in the lipid-lowering effect. The analysis shows that there is a synergistic effect between the effective ingredients in the first filtrate and the second filtrate prepared by a specific method. Therefore, changing the relationship between the total mass of polygonatum, wolfberry, and houttuynia cordata and the total mass of honeysuckle, mint, patchouli, and licorice will affect the effect of the meal replacement powder.

[0059] 2. Detection of total sugar, polyphenols and total flavonoids (1) 5 g of the meal replacement powder of Example 1-2 and Comparative Example 1-7 were added to 50 mL of pure water respectively, placed in a 40°C water bath and stirred to dissolve for 1 hour, and centrifuged to obtain a supernatant, which was then subjected to total sugar determination and polyphenol detection; (2) 5 g of the meal replacement powder of Example 1-2 and Comparative Example 1-7 was added with 50 mL of 60% ethanol aqueous solution (volume fraction), placed in a 40°C water bath and stirred to dissolve for 1 hour. The supernatant was obtained by centrifugation and the total flavonoids were detected.

[0060] The results are shown in Table 2.

[0061] Table 2 Component test results (mass percentage %) test Total Sugar Polyphenols Total flavonoids Example 1 9.4 10.9 16.8 Example 2 9.3 10.8 16.5 Comparative Example 1 5.3 7.6 12.5 Comparative Example 2 8.8 9.6 15.3 Comparative Example 3 7.1 8.9 13.9 Comparative Example 4 6.7 8.7 13.7 Comparative Example 5 6.3 8.5 13.5 Comparative Example 6 6.0 8.2 13.2 Comparative Example 7 5.7 7.9 12.9 Comparing Table 2 with Table 1, the total sugar, polyphenol and total flavonoid content in the meal replacement powder is positively correlated with the lipid-lowering effect, indicating that the lipid-lowering effect of the meal replacement powder is closely related to the content of total sugar, polyphenol and total flavonoid substances in the meal replacement powder. The preparation method of the present invention can release the total sugar, polyphenol and total flavonoid in the raw material to the greatest extent, and changing the preparation process will affect the lipid-lowering effect.

[0062] 3. Stability test The meal replacement powders of Examples 1-2 and Comparative Examples 1-7 were sealed and placed at room temperature (25° C.) for 90 days, and then the taste and color were compared.

[0063] The results are shown in Table 3.

[0064] Table 3 Stability test results test stability Example 1 No bitterness, no color change Example 2 No bitterness, no color change Comparative Example 1 Slightly bitter, darker in color Comparative Example 2 No bitterness, no obvious color change Comparative Example 3 Slightly bitter, darker in color Comparative Example 4 Slightly bitter, darker in color Comparative Example 5 Slightly bitter, darker in color Comparative Example 6 Slightly bitter, darker in color Comparative Example 7 Bitter, darker in color It can be seen from Table 3 that the meal replacement powder prepared by the preparation process of the present invention has better storage stability. It can be seen from Comparative Example 1 that the composition of the raw materials also has a certain influence on the storage stability. Comparative Examples 3-6 prove that changing the preparation process leads to a decrease in the storage stability of the meal replacement powder. The meal replacement powder prepared by the traditional process in Comparative Example 7 has poor storage stability.

[0065] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composite grain meal replacement powder, characterized in that: The invention comprises the following raw materials in parts by weight: 12-16 parts of kudzu root powder, 14-19 parts of poria cocos, 14-18 parts of oats, 11-14 parts of black beans, 5-10 parts of peas, 30-36 parts of black rice, 10-14 parts of bitter buckwheat powder, 3-7 parts of polygonatum, 6-10 parts of wolfberry, 1-4 parts of houttuynia cordata, 3-7 parts of honeysuckle, 1-4 parts of mint, 0.5-1.5 parts of patchouli and 2-5 parts of liquorice.

2. The composite grain meal replacement powder according to claim 1, characterized in that: The ratio of the total mass of polygonatum, wolfberry, and houttuynia cordata to the total mass of honeysuckle, mint, patchouli, and licorice is (15-17): (12-14).

3. The preparation process of the composite grain meal replacement powder according to claim 1 or 2, characterized in that: The following steps are involved: (1) Drying polygonatum, wolfberry and houttuynia cordata separately and then crushing them into less than 20 mesh, mixing them to obtain a first mixed material, adding water to the first mixed material to obtain a first mixed material liquid, adding a composite enzyme, performing enzymolysis at 30-35° C. for 4-7 hours, inactivating the enzyme, obtaining an enzymolysis product, placing the enzymolysis product in a microwave reactor, with a microwave power of 200-250 W, a temperature of 70-75° C., and an extraction time of 1.5-2 hours to obtain a first extract, filtering the extract using a 200-300 mesh filter, and filtering to obtain a first filtrate; (2) The honeysuckle, mint, patchouli and liquorice are dried and crushed to less than 20 meshes respectively, mixed, and then extruded through a screw extruder to obtain a second mixed material; water is added to the second mixed material to obtain a second mixed material liquid, Bifidobacterium longum is added, and anaerobically fermented at 35-38°C for 10-12 hours. After sterilization, Bifidobacterium lactis is continuously added, and anaerobically fermented at 35-38°C for 5-8 hours. After sterilization, Lactobacillus rhamnosus is continuously added, and anaerobically fermented at 35-38°C for 15-17 hours. After sterilization, a second extract is obtained, which is filtered using a 200-300 mesh filter to obtain a second filtrate; (3) mixing the first filtrate and the second filtrate, adding poria cocos, oats, black beans, peas, black rice, and bitter buckwheat, and soaking them to obtain a soaking mixture; (4) adding kudzu root powder to the soaked mixture and grinding the mixture, and passing the ground mixture through a 60-80 mesh sieve to obtain a ground mixture; (5) heating the pulped mixture at 70-75° C. with stirring for 30-40 minutes to obtain a cooked material; (6) The cooked material is subjected to a low-temperature freeze-drying treatment to obtain a dry material, the moisture content of the material is controlled to be 4-6%, the material is crushed, and passed through a 200-mesh sieve to obtain a composite grain meal replacement powder.

4. The preparation process of the composite grain meal replacement powder according to claim 3, characterized in that: The mass of water in step (1) is 8-10 times the mass of the first mixed material.

5. The preparation process of the composite grain meal replacement powder according to claim 3, characterized in that: The complex enzyme in step (1) comprises cellulase, bromelain and pectinase.

6. The preparation process of the composite grain meal replacement powder according to claim 3, characterized in that: In the step (1), the enzyme dosage of cellulase in the first mixed liquid is 100-120 U / mL; the enzyme dosage of bromelain in the first mixed liquid is 70-90 U / mL; and the enzyme dosage of pectinase in the first mixed liquid is 130-150 U / mL.

7. The preparation process of the composite grain meal replacement powder according to claim 3, characterized in that: In step (2), the four-stage temperature gradient of the screw heating is 70-80°C, 100-110°C, 130-140°C, and 160-170°C.

8. The preparation process of the composite grain meal replacement powder according to claim 3, characterized in that: The mass of water in step (2) is 8-10 times the mass of the second mixed material.

9. The preparation process of the composite grain meal replacement powder according to claim 3, characterized in that: The amount of Bifidobacterium longum in the second mixed liquid is 10 6 -10 7 CFU / mL; the amount of Bifidobacterium lactis in the second mixed liquid is 10 10 -10 11 CFU / mL; the amount of Lactobacillus rhamnosus in the second mixed liquid is 10 8 -10 9 CFU / mL.

10. The preparation process of the composite grain meal replacement powder according to claim 3, characterized in that: In the step (3), the mixture is immersed at 40-50° C. for 6-10 hours.