Composition for growing hair and preventing hair loss

Abstract

The invention relates to a composition for growing hair and preventing hair loss, and relates to the technical field of functional food, and the composition is prepared from the following components in parts by mass: 1 part of probiotics, 0.6 to 1.2 parts of radix angelicae sinensis extract, 0.6 to 1.2 parts of herba dendrobii extract, 0.1 to 0.3 part of radix ginseng extract, 0.0000045 to 0.00005 part of biotin, 0.0021 to 0.02 part of nicotinic acid, 0.0017 to 0.012 part of zinc, 0.0000075 to 0.000052 part of selenium and 0.001 to 0.02 part of pyrroloquinoline quinone. The shampoo has the effects of growing hair and preventing alopecia, and can promote the proliferation of hair follicle cells and provide nutrition for hair growth, so that the hair growth is promoted.

Description

Technical Field

[0001] The present application relates to the technical field of functional foods, and in particular to a composition for hair growth and hair loss prevention. Background Art

[0002] Hair has many important functions, including defense, maintaining body temperature and decoration. In recent years, with the intensification of social competition and social pressure, the problem of hair loss has become more serious, which not only affects the appearance, but also may bring psychological burden. There are many reasons for hair loss, including genetics, hormonal imbalance, environmental pollution, improper lifestyle, etc. Both traditional medicine and modern scientific research have shown that the health of hair is closely related to the overall health of the human body, especially the functional status of organs such as the liver, kidneys, spleen and stomach, and the quality of blood circulation.

[0003] Clinically, a variety of methods are usually used to deal with the problem of hair loss. Common treatments include drug therapy, laser therapy, hair transplant surgery, etc. Among them, drug therapy is one of the most commonly used methods, such as finasteride and minoxidil, which achieve the purpose of preventing and treating hair loss by improving the scalp microenvironment or promoting hair follicle regeneration. In addition, some natural therapies are widely used, such as using shampoos containing natural ingredients, massaging the scalp, adjusting eating habits, etc. However, these methods often take a long time to be effective, and are prone to relapse after stopping the medication.

[0004] Although existing treatments can alleviate the symptoms of hair loss to a certain extent, there are still some obvious limitations and shortcomings. For example, drug treatments may have side effects, and long-term use may cause skin allergies or other adverse reactions; hair transplant surgery is expensive and postoperative care is complicated, which is not suitable for everyone. Therefore, it is necessary to develop a safe, effective, easy-to-use composition for hair growth and hair loss prevention. Summary of the invention

[0005] In order to enhance the effects of hair growth and hair loss prevention, the present application provides a composition for hair growth and hair loss prevention.

[0006] The present application provides a composition for hair growth and hair loss prevention using the following technical solution: A composition for hair growth and hair loss prevention, comprising the following components in parts by weight: 1 serving of probiotics 0.6-1.2 parts of Angelica extract Dendrobium extract 0.6-1.2 parts Ginseng extract 0.1-0.3 parts Biotin 0.0000045-0.00005 parts Nicotinic acid 0.0021-0.02 parts Zinc 0.0017-0.012 parts Selenium 0.0000075-0.000052 parts Pyrroloquinoline quinone 0.001-0.02 parts.

[0007] Probiotics can relieve stress through the neuroendocrine system, immune system, and vagus nerve system, thereby correcting the disorder of the nervous system, reducing anxiety, sadness, mental tension, and emotional instability, and forming a gut-hair axis. By selecting probiotics that can relieve emotions and reduce stress to form a gut-hair axis, stress can be reduced, while supplying nutrients to hair follicles, promoting the proliferation of hair follicles, and maintaining the morphological structure of hair follicles, thereby promoting hair growth. Angelica extract, dendrobium extract, and ginseng extract are rich in a variety of active ingredients, which enter the blood circulation after being absorbed by the intestines; antioxidant substances such as ferulic acid in angelica extract can remove free radicals in the body, reduce the oxidative stress faced by hair follicle cells during growth, protect the DNA integrity of hair follicle cells, and ensure their normal proliferation and differentiation. Angelica can also reduce cavitation of hair follicle cells, increase the number of hair follicles per unit field of view, reduce inflammatory reactions of hair follicles, and relieve hair follicle damage; nutrients in dendrobium extract can nourish and moisturize hair follicles, reduce apoptosis of hair follicle cells, promote hair follicle activation, protect the morphological structure of hair follicles, and promote the regeneration of hair follicle blood vessels; ginseng extract can reduce the degree of damage to hair follicle papilla cells, inhibit hair follicle degeneration, thereby stimulating the proliferation of outer root sheath keratinocytes and reducing hair loss; Biotin, as a coenzyme, deeply participates in key biochemical processes such as fat metabolism and amino acid metabolism in the body, accurately synthesizes various lipids and proteins required for hair follicle construction, and lays a solid foundation for new hair; niacin participates in intracellular redox reactions, efficiently generates ATP, and continuously supplies energy for the active growth of hair follicle cells, ensuring the healthy growth of hair; zinc integrates into the cellular metabolic network, activates a series of enzymes related to hair follicle development, and finely regulates hormone levels, allowing hair follicles to thrive in a suitable endocrine atmosphere; selenium, with its own dual characteristics of antioxidant and immunomodulatory, cleverly balances the local immune response of the scalp while fighting free radicals and repairing oxidative damage, avoiding accidental damage to hair follicles caused by immune overreaction; pyrroloquinoline quinone, with its powerful antioxidant power, scavenges free radicals, protects hair follicle cells from oxidative stress damage, maintains the mitochondrial function of hair follicle cells, and at the same time increases the number of mitochondria in hair follicle cells, improves the efficiency of mitochondrial energy metabolism, promotes normal hair growth and prevents hair loss, and synergizes with other components in the composition to jointly create a healthy hair follicle microenvironment from multiple aspects such as nutrient supply and metabolic regulation, and enhances the overall hair growth and anti-hair loss effects.

[0008] Preferably, the probiotics include Bifidobacterium longum One or more of Lactobacillus paracasei LC85, Pediococcus acidilactici PA53, Bifidobacterium adolescentis BAC30, Bifidobacterium animalis lactis subspecies BLA80, Lactobacillus casei LC16, Lactobacillus plantarum SG5, Lactobacillus helveticus L551, Lactobacillus paracasei L578, Streptococcus thermophilus S709 and Lactobacillus plantarum P8, wherein the total number of viable bacteria in the probiotics is not less than 10 billion / g.

[0009] Bifidobacterium longum It can directly hit the source of stress and accurately regulate the secretion of cortisol when the human body is under stress, thereby effectively weakening the psychological and physiological response of healthy people to stress, not only reducing the anxiety level after stress, but also reducing the daily stress feeling; Lactobacillus paracasei LC85, Pediococcus acidilactici PA53 strain, Bifidobacterium adolescentis BAC30, Bifidobacterium animalis lactis subspecies BLA80 and Lactobacillus casei LC16 can relieve anxiety and reduce stress by increasing the content of 5-hydroxytryptamine and regulating the balance between γ-aminobutyric acid (GABA) and glutamate (GLU); Lactobacillus plantarum SG5 can further enhance the effect of relieving anxiety and reducing stress by increasing the content of GABA in the body; Lactobacillus helveticus L551, Lactobacillus paracasei L578, and Streptococcus thermophilus S709 regulate neurotransmitters (GABA, L-serine, acetylcholine, 5-hydroxytryptamine), antioxidants (glutamine, proline, betaine, NAD+), and anti-inflammatory substances (valine, propionic acid, betaine) in the body, and regulate from multiple dimensions such as neural regulation, anti-oxidative stress, and anti-inflammation, thereby achieving the effect of relieving anxiety and reducing stress; Lactobacillus plantarum P8 goes deep into the bottom layer of cellular metabolism, and through fine-tuning 43 pathways such as amino acid metabolism, vitamin synthesis, transferase synthesis, peptidoglycan biosynthesis, and nucleotide biosynthesis, it consolidates and enhances the effectiveness of relieving anxiety and reducing stress from the inside out, and enhances the hair growth and anti-hair loss effects of the combination.

[0010] Preferably, the probiotics are encapsulated, and the wall material of the encapsulation comprises sodium alginate, chitosan and hydroxypropyl methylcellulose phthalate.

[0011] The double-layer encapsulation using the cross-linking reaction of sodium alginate and chitosan can not only physically isolate adverse external factors, but also use the biocompatibility of the two to create a suitable microenvironment for probiotics; the introduction of hydroxypropyl methylcellulose phthalate as the outer coating uses the characteristics of hydroxypropyl methylcellulose phthalate being stable and insoluble in the gastric acid environment and soluble in the weak alkaline environment of the intestine. When the embedded microspheres enter the stomach, hydroxypropyl methylcellulose phthalate can resist the erosion of gastric acid and prevent the probiotics from being damaged by the acidic environment. Then, in the intestine, the environment becomes weakly alkaline, and hydroxypropyl methylcellulose phthalate dissolves, releasing the probiotics protected by the double-wall material inside, allowing them to accurately colonize the intestine, regulate the intestinal flora, regulate emotions through the gut-brain axis, relieve stress-induced hair loss factors, and at the same time optimize the intestinal flora, help nutrient absorption, and allow zinc, selenium and other key nutrients for hair growth, as well as plant extracts such as angelica and ginseng to better play their role, providing nourishment to hair follicles in all directions, and ultimately effectively promoting hair growth and preventing hair loss.

[0012] Preferably, the composition further comprises 0.01-0.1 parts of pea sprout extract; and the crude drug ratio of the angelica extract, dendrobium extract, ginseng extract and pea sprout extract is 10:1.

[0013] Pea sprout extract is rich in various vitamins, such as vitamin A, C, E, etc., which can directly participate in the removal of harmful free radicals in the body, and synergize with ferulic acid in angelica extract to reduce the oxidative damage suffered by hair follicle cells, ensure the normal physiological function of hair follicle cells, maintain their DNA stability, and effectively reduce the cavitation of hair follicle cells; the minerals in pea sprout extract, such as zinc, iron and other trace elements, are important components of enzymes related to hair follicle development, or can stimulate their activity, thereby accurately regulating the biochemical reactions in hair follicle cells and promoting the proliferation and differentiation of hair follicle cells; pea sprout extract The plant active peptides contained in it have excellent nourishing properties, and work synergistically with the dendrobium extract to enhance the vitality of hair follicles, inhibit the apoptosis of hair follicle cells, and stimulate the angiogenesis of hair follicles, providing sufficient nutrition for the hair follicles, thereby enhancing the hair growth and anti-hair loss effects of the composition; the active ingredients in the extract obtained according to the above-mentioned crude drug ratio can reach a synergistic optimal concentration in the blood circulation after entering the human body, which not only avoids the waste of resources or possible side effects caused by excessive amounts of a certain ingredient, but also prevents a certain ingredient from being too little and unable to fully exert its efficacy, thereby achieving multi-dimensional hair growth and anti-hair loss effects in an all-round, precise and efficient manner.

[0014] In a preferred embodiment, the angelica extract, dendrobium extract, ginseng extract and pea sprout extract are embedded in pH-responsive nanoliposomes to obtain liposome-embedded extracts, and the raw materials for preparing the pH-responsive nanoliposomes include phosphatidylserine, dioleoylphosphatidylethanolamine and soybean lecithin.

[0015] Phosphatidylserine has a unique molecular structure and carries a negative charge, which enables the liposomes to remain stable in the complex electrolyte environment of the gastrointestinal tract, effectively preventing the liposomes from aggregating and precipitating, and ensuring that the encapsulated angelica extract, dendrobium extract, ginseng extract and pea sprout extract safely reach the intestine; at the same time, phosphatidylserine can interact with the cell membrane phospholipid bilayer, making the extracts easier to be taken up by intestinal cells; dioleoylphosphatidylethanolamine gives the liposomes good flexibility, allowing them to flexibly adapt to gastrointestinal motility and environmental changes, and avoid rupture due to mechanical extrusion or chemical stimulation; soybean lecithin is amphiphilic and can emulsify hydrophilic extract components and hydrophobic components, thereby improving the uniformity of dispersion of each extract in the liposome; encapsulating the extracts through liposomes constructed with these three substances not only ensures the activity of the extracts and improves their intestinal absorption efficiency, but also creates a better intestinal microecological environment for probiotics, promotes the proliferation and metabolism of probiotics, enables them to better regulate intestinal function, and synergistically improves hair growth and anti-hair loss performance.

[0016] In a preferred embodiment, the biotin is modified with oleic anhydride to obtain esterified biotin.

[0017] Oleic anhydride has long-chain fatty acid groups. After esterification reaction with biotin, the chemical structure of biotin is optimized; the lipophilicity of esterified biotin is enhanced, and in the lipid environment of the intestine, it can be more closely attached to the lipid bilayer of the intestinal mucosal cell membrane, reducing transmission obstacles in the intestine and promoting its absorption by the intestine; the long-chain fatty acid groups enhance the stability of biotin in the complex chemical environment of the intestine, enabling it to effectively resist the destruction of biotin by potential factors such as gastric acid, digestive enzymes and intestinal microorganisms, thereby better exerting its coenzyme function, deeply participating in key biochemical reactions such as fat metabolism and amino acid metabolism, and thus enhancing hair growth and anti-hair loss properties.

[0018] In a preferred embodiment, the nicotinic acid is modified with glucose to obtain glycosylated nicotinic acid.

[0019] Niacin is modified with glucose to form glycosylated niacin, which changes the molecular structure of niacin. Glycosylated niacin can release niacin more stably and continuously with the help of the metabolic pathway of glucose, thereby protecting the active center of niacin and preventing it from being prematurely metabolized or inactivated before reaching the target site. Glycosylation modification makes niacin easier to be absorbed and utilized by cells, and the slowly released niacin can play its role in promoting local blood circulation and regulating the metabolism of hair follicle cells, providing a more favorable environment for hair growth, thereby enhancing the effects of hair growth and preventing hair loss.

[0020] Preferably, the source of zinc includes one or more of zinc sulfate, zinc citrate trihydrate, zinc citrate, zinc gluconate, zinc oxide, zinc lactate, zinc acetate and zinc chloride.

[0021] The zinc element in the above substances is easily absorbed, can effectively supply new elements, promote the production of hair keratin, regulate hormone levels, and assist in removing free radicals, thereby reducing the damage of oxidative stress to hair follicle cells, and synergizes with the components in the composition to enhance hair growth and anti-hair loss performance.

[0022] Preferably, the source of selenium includes one or more of sodium selenite, selenium-enriched yeast, L-seleno-methylselenocysteine, selenocysteine, selenized carrageenan and selenoprotein.

[0023] The selenium in the above substances is easily absorbed, and can effectively remove free radicals in hair follicles and surrounding tissues, participate in immune regulation, help maintain local immune balance of the scalp, avoid inflammatory reactions caused by immune excess, and reduce the risk of immune-related diseases; selenium is also closely linked to the metabolism of thyroid hormones, which can regulate the growth cycle of hair follicles. An appropriate amount of selenium ensures normal synthesis and conversion of thyroid hormones, so that hair follicles can transition from the resting phase to the growth phase in an orderly manner under appropriate hormonal stimulation, thereby promoting hair regeneration and enhancing the hair growth and anti-hair loss properties of the composition.

[0024] Preferably, the dosage form of the composition includes any one of capsules, tablets, granules, powders, gel candies, and oral liquids, and the excipients of the composition include fillers, disintegrants, lubricants, binders, sweeteners, flavoring agents, acid-base regulators, preservatives, and gelling agents.

[0025] The filler is selected from maltodextrin, starch, pregelatinized starch, lactose, mannitol, chitosan, microcrystalline cellulose, sucrose and the like.

[0026] The disintegrant is selected from starch, pregelatinized starch, microcrystalline cellulose, sodium carboxymethyl starch, cross-linked polyvinyl pyrrolidone, low-substituted hydroxypropyl cellulose, cross-linked sodium carboxymethyl cellulose and the like.

[0027] The lubricant is selected from magnesium stearate, sodium lauryl sulfate, talc, silicon dioxide and the like.

[0028] The binder is selected from starch slurry, polyvinyl pyrrolidone, hydroxypropyl methylcellulose and the like.

[0029] The sweetener is selected from neotame, aspartame, sucrose, cyclamate, ammonium glycyrrhizinate, oligosaccharides, lactitol, stevioside, erythritol, sorbitol, sucralose, mogroside and the like.

[0030] The flavoring agent is selected from sweeteners and various flavors.

[0031] The acid-base regulator is selected from citric acid, sodium citrate, malic acid, lactic acid, tartaric acid, sodium bicarbonate and the like.

[0032] The preservative is selected from benzoic acid, sodium benzoate, sorbic acid and its potassium salt and the like.

[0033] The gelling agent is selected from carrageenan, gelatin, agar, modified starch, pectin, gum and the like.

[0034] In summary, the present application includes at least one of the following beneficial technical effects: 1. By combining probiotics with angelica, dendrobium, ginseng, biotin, niacin, zinc, selenium and pyrroloquinoline quinone in a certain proportion and making a suitable dosage form, after the probiotics enter the human body, they relieve stress through the neuroendocrine system, immune system and vagus nerve system, thereby correcting the disorder of the nervous system, reducing anxiety, sadness, mental tension and emotional instability, and forming a gut-hair axis; zinc, selenium, biotin and niacin can promote the proliferation of hair follicle cells, provide nutrition for hair growth, thereby promoting hair growth and achieving the effect of preventing hair loss.

[0035] 2. Phosphatidylserine has a unique molecular structure and carries a negative charge, which allows the liposome to remain stable in the complex electrolyte environment of the gastrointestinal tract, effectively preventing the aggregation and precipitation of the liposomes, and ensuring that the embedded angelica extract, dendrobium extract, ginseng extract and pea sprout extract safely reach the intestine; at the same time, phosphatidylserine can interact with the cell membrane phospholipid bilayer, making the extract easier to be taken up by intestinal cells; dioleoylphosphatidylethanolamine gives the liposome good flexibility, allowing it to flexibly adapt to gastrointestinal motility and environmental changes, and avoid rupture due to mechanical extrusion or chemical stimulation; soybean lecithin is amphiphilic and can emulsify hydrophilic extract components and hydrophobic components, thereby improving the uniformity of dispersion of each extract in the liposome; encapsulating the extract with the liposome constructed by these three substances not only ensures the activity of the extract and improves its intestinal absorption efficiency, but also creates a better intestinal microecological environment for probiotics, promotes the proliferation and metabolism of probiotics, enables them to better regulate intestinal function, and synergistically improves hair growth and anti-hair loss performance.

[0036] 3. Oleic anhydride has long-chain fatty acid groups. After esterification reaction with biotin, the chemical structure of biotin is optimized; the lipophilicity of esterified biotin is enhanced, and in the lipid environment of the intestine, it can be more closely attached to the lipid bilayer of the intestinal mucosal cell membrane, reducing transmission obstacles in the intestine and promoting its absorption by the intestine; the long-chain fatty acid groups enhance the stability of biotin in the complex chemical environment of the intestine, enabling it to effectively resist the destruction of biotin by potential factors such as gastric acid, digestive enzymes and intestinal microorganisms, thereby better exerting its coenzyme function, deeply participating in key biochemical reactions such as fat metabolism and amino acid metabolism, and thus enhancing hair growth and anti-hair loss performance.

[0037] 4. Nicotinic acid is modified with glucose, and the resulting glycosylated niacin changes the molecular structure of niacin. Glycosylated niacin can release niacin more stably and continuously with the help of the metabolic pathway of glucose, thereby protecting the active center of niacin and preventing it from being metabolized or inactivated prematurely before reaching the target site. Glycosylation modification makes niacin easier to be absorbed and utilized by cells, and the slowly released niacin can play its role in promoting local blood circulation, regulating the metabolism of hair follicle cells, etc., providing a more favorable environment for hair growth, thereby enhancing the effects of hair growth and preventing hair loss. DETAILED DESCRIPTION

[0038] The present application discloses a composition for hair growth and hair loss prevention. The raw materials used in the present application can be obtained from commercially available raw materials unless otherwise specified. The present application is further described in detail below in conjunction with the examples: Raw material description: The composition of the probiotics in the present application example includes Bifidobacterium longum Lactobacillus paracasei LC85 and Lactobacillus plantarum SG5, angelica extract, dendrobium extract, ginseng extract and pea sprout extract were all added in a crude drug ratio of 10:1, zinc was added in the form of zinc gluconate (CAS No.: 4468-02-4), selenium was added in the form of selenocysteine, pyrroloquinoline quinone (CAS: 72909-34-3), sodium alginate (CAS No.: 9005-38-3), chitosan (CAS No.: 9012-76-4), hydroxypropyl methylcellulose phthalate (CAS No.: 9050-31-1), microcrystalline cellulose (CAS No.: 9004-34-6), cross-linked sodium carboxymethyl cellulose (CAS No.: 7481 1-65-7), polyvinyl pyrrolidone (CAS No.: 9003-39-8), low-substituted hydroxypropyl cellulose (CAS No.: 9004-64-2), cross-linked polyvinyl pyrrolidone (CAS No.: 25249-54-1), hydroxypropyl methylcellulose (CAS No.: 9004-65-3), soybean lecithin (CAS No.: 8030-76-0), phosphatidylserine (CAS No.: 51446-62-9), dioleoylphosphatidylethanolamine (CAS No.: 4004-05-1), biotin (CAS No.: 58-85-5), oleic anhydride (CAS No.: 24909-72-6), niacin (CAS No.: 59-67-6).

[0039] Preparation Example 1 Encapsulated Probiotics 1 g of sodium alginate was dissolved in 100 mL of deionized water, and stirred at 200 rpm for 30 min to obtain a sodium alginate solution; 0.5 g of chitosan was dissolved in 50 mL of a 2% dilute acetic acid solution, and stirred at 200 rpm for 40 min to obtain a chitosan solution; the sodium alginate solution and the chitosan solution were mixed, and the mixture was heated to 50° C. at a stirring speed of 200 rpm to obtain a mixed solution; a physiological saline suspension containing 1 g of probiotics was added to the mixed solution, and the mixture was stirred at 350 rpm while being added dropwise. The mixture was stirred and ultrasonicated for 5 minutes to obtain a mixed suspension; the mixed suspension was dripped into a solution containing 500 mL of 0.1 mol / L calcium chloride at a speed of 10 drops / s, the cross-linking reaction was carried out for 30 minutes, and the preliminary embedded probiotics were obtained by filtering, and the washed preliminary embedded probiotics were placed in 100 mL of 3 g / L hydroxypropyl methylcellulose phthalate ethanol solution, stirred at a speed of 200 rpm for 30 minutes, and the encapsulated product was obtained by filtering, and the encapsulated product was washed with deionized water and vacuum dried at 40°C to obtain the embedded probiotics.

[0040] Example 1 The hair growth and anti-hair loss composition, microcrystalline cellulose and cross-linked sodium carboxymethyl cellulose were poured into a mixer and stirred at a speed of 100 rpm for 15 min. A polyvinyl pyrrolidone solution with a concentration of 10% was added and stirred at a speed of 150 rpm for 20 min to obtain a soft material. The probiotics in the hair growth and anti-hair loss composition were the embedded probiotics prepared in Preparation Example 1. The soft material was granulated through a sieve to obtain wet granules. The wet granules were placed in a drying oven and dried at 60° C. to a moisture content of 2% to obtain dried granules. The dried granules were sieved and sized, magnesium stearate and silicon dioxide were added, and after mixing evenly, they were pressed into tablets using a tablet press.

[0041] Table 1 Ingredients and contents of the hair growth and anti-hair loss composition in tablets Example 2 The hair growth and hair loss prevention composition, pregelatinized starch, lactose and low-substituted hydroxypropyl cellulose were mixed and stirred at a speed of 150 rpm for 15 minutes, starch slurry was added, and the mixture was stirred at a speed of 200 rpm to form a soft material, wherein the probiotics in the hair growth and hair loss prevention composition were the embedded probiotics prepared in Preparation Example 1; the soft material was made into wet granules by a granulator, and dried at 50° C. to a water content of 2% to obtain dried granules, the dried granules were sieved and sized, mixed evenly at a speed of 100 rrpm, and then filled into hollow capsules by a capsule filling machine to prepare capsules.

[0042] Table 2 Ingredients and contents of the hair growth and anti-hair loss composition in the capsule Element Dosage range (portions) Probiotics 1 Angelica Extract 0.9 Dendrobium officinale extract 0.9 Ginseng Extract 0.2 Pea sprout extract 0.01 Biotin 0.00002725 niacin 0.01105 Zinc 0.00685 selenium 0.00002975 Pyrroloquinoline quinone 0.0105 Example 3 The hair growth and hair loss prevention composition, maltodextrin, starch, and cross-linked polyvinyl pyrrolidone were stirred and mixed in a stirrer at a speed of 150 rpm for 10 minutes, a 10% hydroxypropyl methylcellulose solution was added, and the mixture was stirred at a speed of 200 rpm to form a soft material, wherein the probiotics in the hair growth and hair loss prevention composition are the embedded probiotics prepared in Preparation Example 1; the soft material was made into wet granules by a swinging granulator, and the wet granules were dried in a fluidized bed dryer at 50° C. to a water content of 2% to obtain dried granules; the dried granules were sieved and granulated, silicon dioxide, stevioside, mint essence, lactic acid, and potassium sorbate were added, and the mixture was stirred evenly at a speed of 100 rpm, and then packaged to prepare granules.

[0043] Table 3 Ingredients and contents of the hair growth and anti-hair loss composition in the granules Preparation Example 2 Preparation of liposome-embedded extract 2.4 g of angelica extract, 2.4 g of dendrobium extract, 0.2 g of ginseng extract, and 0.11 g of pea sprout extract were mixed and dispersed in 20 mL of phosphate buffer (pH 7.4) to obtain an aqueous phase; 3 g of soybean lecithin, 1 g of phosphatidylserine, and 1 g of dioleoylphosphatidylethanolamine were dispersed in 20 mL of ethanol, and stirred at 25° C. and 300 rpm for 30 min to obtain an organic phase; the organic phase was added dropwise to the aqueous phase at a speed of 0.5 mL / min, and the stirring speed was maintained at 300 rpm for 60 min to obtain an emulsion; the emulsion was spray-dried with an inlet air temperature of 160° C. and an outlet air temperature of 80° C. to obtain a liposome-encapsulated extract.

[0044] Preparation Example 3 Preparation of esterified biotin 1 g of biotin, 2 g of oleic anhydride and 0.5 g of anhydrous pyridine were stirred in an oil bath at 70°C at 200 rpm for 4 h, and the mixture was cooled to below 30°C to obtain a crude product. The crude product was poured into ice water for quenching, and stirred to allow a white precipitate to precipitate. The precipitate was collected by filtration, washed with ice water, and vacuum dried at 50°C to constant weight to obtain esterified biotin.

[0045] Preparation Example 4 Preparation of glycosylated nicotinic acid 1 g of nicotinic acid was dissolved in 20 mL of phosphate buffer (pH 7.4), 1.8 g of glucose was added, and the mixture was stirred evenly at a speed of 200 rpm to obtain a mixed solution; uridine diphosphate-glucosyltransferase (30 U enzyme / g nicotinic acid) was added to the mixed solution, and the mixture was reacted at a speed of 120 rpm in a constant temperature shaker at 35° C. for 6 hours to obtain a reaction solution, the reaction solution was heated at 85° C. for 10 minutes to inactivate the enzyme, the supernatant was extracted by centrifugation, the supernatant was concentrated under reduced pressure, anhydrous ethanol was added for precipitation, the precipitate was collected by centrifugation, and vacuum dried at 60° C. to obtain glycosylated nicotinic acid.

[0046] Example 4 Example 4 is based on Example 3. The only difference between Example 4 and Example 3 is that in Example 4, the angelica extract, dendrobium extract, ginseng extract, and pea sprout extract in the hair growth and anti-hair loss composition are replaced with the liposome-encapsulated extract containing an equal amount of extracts prepared in Preparation Example 2.

[0047] Example 5 Example 5 is based on Example 3. The only difference between Example 5 and Example 3 is that in Example 5, the biotin in the hair growth and hair loss prevention composition is replaced with an equimolar amount of esterified biotin prepared in Preparation Example 3.

[0048] Example 6 Example 6 is based on Example 3. The only difference between Example 6 and Example 3 is that in Example 6, the nicotinic acid in the hair growth and hair loss prevention composition is replaced by an equal molar amount of glycosylated nicotinic acid prepared in Preparation Example 4.

[0049] Example 7 Example 7 is based on Example 3, and the only difference between Example 7 and Example 3 is that in Example 7, pea sprout extract is not added.

[0050] Comparative Example 1 Comparative Example 1 is based on Example 3, and the only difference between Comparative Example 1 and Example 3 is that pyrroloquinoline quinone is not added in Comparative Example 1.

[0051] Performance testing Male C57BL / 6 mice aged 6-8 weeks and weighing 20-25g were selected, and 8% sodium sulfide solution was applied to the back skin of the mice. After staying for 5-8 minutes, the mice were rinsed with warm water and repeated 2-3 times to induce a hair loss model. The mice treated with hair loss were randomly divided into groups, with 8 mice in each group. The mice with hair loss were given the sample according to the equivalent dose, once a day for 8 consecutive weeks as the sample group; a group of mice were given finasteride once a day, with a dosage of 0.15mg / kg, for 8 consecutive weeks as the positive control group; the blank control group was given an equal amount of normal saline, and the treatment method was the same as the experimental group.

[0052] Index determination: (1) Detection of new hair length of mice: After 8 weeks, 10 new hairs were randomly picked from the experimental area of ​​each mouse using tweezers and a magnifying glass, and the lengths of the 10 new hairs were measured using a vernier caliper. The mean length of the new hair of each mouse was calculated, and finally the mean of each group was calculated. The results are recorded in Table 4.

[0053] (2) Detection of the weight of newly grown hair in mice: On the last day of drug administration, all newly grown hair was shaved off with a shaver, weighed, and the average weight of newly grown hair in each group of mice (including the weight of 10 hairs of the above-mentioned measured length) was calculated, which was counted as Y. The results are recorded in Table 4.

[0054] (3) Detection of hair follicle number in mice: After 8 weeks, all mice were killed at the end of the experiment. The skin of the experimental area on the back with shaved hair was taken and fixed with 10% formalin. The cross-section of the hair follicles was taken and the number of hair follicles was observed under a microscope. Three fields of view were randomly selected under the condition of *100, and the number of hair follicles was counted. The average of the three fields of view was taken as a sample hair follicle count, which was calculated as Z. The results are recorded in Table 4.

[0055] (4) Standard score calculation: When scoring the test samples, the above three indicators are comprehensively considered and the standard score calculation method is adopted. The specific calculation process is as follows: Take the average hair length (X i ) The difference between each group of data and the blank control group (X0) is expressed as (X i -X0) the maximum difference is 100 points, the blank control group is 0 points (the blank group is also counted as the index group, that is, the blank control group minus the blank control group, the score is 0, and the standard score is 0 points. If the data of the sample group is less than the data of the blank group, it means that the sample has an inverse effect and is directly removed). The scores of the other groups are: (X i -X0) / (X i -X0) Max *100. The average hair weight Y and the average number of hair follicles Z are calculated in the same way. Among the various factors, hair follicles are the key factor in hair growth, so they are given a weight of 40%, and the hair length index and hair weight index are each given a weight of 30%. The final score of each group is calculated according to the following formula: Group score = (X i -X0) / (X i -X0) Max *100*30%+(Y i -Y0) / (Y i -Y0) Max *100*30%+(Z i -Z0) / (Z i -Z0) Max *100*40%.

[0056] Table 4 Test results of hair growth and anti-hair loss performance As can be seen from Table 4, the average hair length of Examples 1-3 is greater than 7.06±0.23 mm, the average hair weight is greater than 33.25±1.51 mg, and the average number of hair follicles is greater than 20.1±0.52. Compared with the blank control group, the hair growth and anti-hair loss effects are significantly improved. It can be seen that the hair growth and anti-hair loss composition prepared in the present application has good hair growth and anti-hair loss performance.

[0057] As can be seen from Table 4, the only difference between Example 4 and Example 3 is that the extract is embedded in Example 4, and the hair growth and anti-hair loss performance of Example 4 is significantly improved compared with Example 3; this is because the use of liposomes to embed the extract can effectively protect the active substances in the extract and promote its absorption and utilization in the intestine, thereby effectively improving its hair growth and anti-hair loss performance.

[0058] As can be seen from Table 4, the only difference between Example 5 and Example 3 is that in Example 5, biotin is esterified, and compared with Example 3, Example 5 has improved hair growth and anti-hair loss performance; this is because the lipophilicity of esterified biotin is enhanced, it is easier to be absorbed by the intestine, and its stability is improved, so that it can better exert its performance.

[0059] As can be seen from Table 4, the only difference between Example 6 and Example 3 is that nicotinic acid is glycosylated in Example 6, and compared with Example 3, the hair growth and anti-hair loss performance of Example 6 is improved; this is because the glycosylation modification can protect the active center of nicotinic acid and promote the absorption and utilization of nicotinic acid by cells, thereby improving the hair growth and anti-hair loss performance.

[0060] As can be seen from Table 4, the only difference between Example 7 and Example 3 is that: No pea sprout extract is added in Example 7, and compared with Example 3, the hair growth and anti-hair loss performance of Example 7 is reduced; this is because the pea sprout extract is not added, and the synergistic effect between the extracts is weakened due to the lack of the addition of the pea sprout extract, which affects the hair growth and anti-hair loss effects.

[0061] As can be seen from Table 4, the only difference between Comparative Example 1 and Example 3 is that pyrroloquinoline quinone is not added in Comparative Example 1, and the hair growth and hair loss prevention effects of Comparative Example 1 are reduced compared with Example 3; this is because the lack of pyrroloquinoline quinone reduces the antioxidant stress capacity and weakens the synergistic effect, thereby reducing the hair growth and hair loss prevention performance.

[0062] This specific embodiment is only an explanation of the present application, and it is not a limitation of the present application. Through the above description, relevant staff can make various changes and modifications without deviating from the technical idea of ​​the present application. The technical scope of the present application is not limited to the content in the specification, and its technical scope must be determined according to the scope of the claims.

Claims

1. A composition for hair growth and hair loss prevention, characterized in that: The composition includes the following components in parts by weight: 1 serving of probiotics 0.6-1.2 parts of Angelica extract Dendrobium extract 0.6-1.2 parts Ginseng extract 0.1-0.3 parts Biotin 0.0000045-0.00005 parts Nicotinic acid 0.0021-0.02 parts Zinc 0.0017-0.012 parts Selenium 0.0000075-0.000052 parts Pyrroloquinoline quinone 0.001-0.02 parts.

2. A composition for hair growth and hair loss prevention according to claim 1, characterized in that: The probiotics include one or more of Bifidobacterium longum 1714-Serenitas®, Lactobacillus paracasei LC85, Pediococcus acidilactici PA53 strain, Bifidobacterium adolescentis BAC30, Bifidobacterium animalis lactis subspecies BLA80, Lactobacillus casei LC16, Lactobacillus plantarum SG5, Lactobacillus helveticus L551, Lactobacillus paracasei L578, Streptococcus thermophilus S709 and Lactobacillus plantarum P8, and the total number of live bacteria in the probiotics is not less than 10 billion / g.

3. A composition for hair growth and hair loss prevention according to claim 2, characterized in that: The probiotics are subjected to embedding treatment, and the embedding wall material comprises sodium alginate, chitosan and hydroxypropyl methylcellulose phthalate.

4. A composition for hair growth and hair loss prevention according to claim 1, characterized in that: The composition also includes 0.01-0.1 parts of pea seedling extract; the crude drug ratio of the angelica extract, the dendrobium extract, the ginseng extract and the pea seedling extract is 10:

1.

5. A composition for hair growth and hair loss prevention according to claim 4, characterized in that: The angelica extract, dendrobium extract, ginseng extract and pea seedling extract are embedded in pH-responsive nanoliposomes to obtain liposome-embedded extracts. The raw materials for preparing the pH-responsive nanoliposomes include phosphatidylserine, dioleoylphosphatidylethanolamine and soybean lecithin.

6. A composition for hair growth and hair loss prevention according to claim 1, characterized in that: The biotin is modified with oleic anhydride to obtain esterified biotin.

7. A composition for hair growth and hair loss prevention according to claim 1, characterized in that: The nicotinic acid is modified with glucose to obtain glycosylated nicotinic acid.

8. A composition for hair growth and hair loss prevention according to claim 1, characterized in that: The zinc source includes one or more of zinc sulfate, zinc citrate trihydrate, zinc citrate, zinc gluconate, zinc oxide, zinc lactate, zinc acetate and zinc chloride.

9. A composition for hair growth and hair loss prevention according to claim 1, characterized in that: The source of selenium includes one or more of sodium selenite, selenium-enriched yeast, L-seleno-methylselenocysteine, selenocysteine, selenized carrageenan and selenoprotein.

10. A composition for hair growth and hair loss prevention according to any one of claims 1 to 9, characterized in that: The dosage form of the composition includes any one of capsules, tablets, granules, powders, gel candies, and oral liquids, and the auxiliary materials of the composition include fillers, disintegrants, lubricants, adhesives, sweeteners, flavoring agents, acid-base regulators, preservatives, and gelling agents.

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