Anti-wrinkle firming and emollient cosmetic and method of making same

Abstract

The application belongs to the technical field of cosmetics, and particularly relates to an anti-wrinkle firming and moisturizing cosmetic and a preparation method thereof. The anti-wrinkle firming and moisturizing cosmetic is prepared from the following raw materials in percentage by weight: 5.5%-6.5% of a composite polypeptide freeze-dried powder, 2%-3% of plant extracts, 7%-9% of a humectant, 2.5%-3.5% of an emulsifier, 10%-15% of a moisturizing agent, 0.1%-0.3% of a preservative, and water to make up the rest to 100%. The application selects specific polypeptide compounding and freeze-drying treatment, scientifically extracts effective components in plants, and combines a molecular weight fractionated hyaluronic acid compound humectant and a nano-liposome moisturizing agent, so that the prepared cosmetic can achieve the effects of anti-wrinkle firming and moisturizing at the same time, and has good use feeling and high stability.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to an anti-wrinkle firming and moisturizing cosmetic and a preparation method thereof. BACKGROUND

[0002] With the improvement of people's living standards, the demand for skin care is increasing. As the largest organ of the human body, the skin will inevitably age with age. In addition, environmental factors such as light, radiation, pollution, and heavy work pressure and irregular work and rest will also accelerate the aging of the skin. Skin aging is mainly manifested in abnormal keratin layer, increased wrinkles, reduced skin elasticity, dryness, and thinning skin.

[0003] At present, there are various types of cosmetics on the market. Taking face cream as an example, there are various types, mainly including moisturizing cream, whitening cream, anti-aging cream, etc. Moisturizing cream can keep the skin moist, whitening cream can improve the skin color, and anti-aging cream can reduce the appearance of fine lines and wrinkles. However, the effects of various creams are relatively single, such as acting only on moisturizing, or only for anti-wrinkle repair, or only for whitening and freckle lightening, which cannot meet the needs of consumers for all-around skin care.

[0004] At the same time, the existing anti-wrinkle firming cream has some problems. In the prior art, for anti-wrinkle firming, some products add polypeptide ingredients to promote collagen production and improve wrinkles to a certain extent, but the effect of single polypeptide is limited, and the activity of polypeptide is easily affected in complex formula system and storage process, resulting in reduced efficacy. At the same time, some products that claim to have firming effect often rely on certain chemical synthetic ingredients, which can produce a sense of firmness in the skin in the short term, but long-term use may cause irritation to the skin, posing a safety hazard.

[0005] In terms of moisturizing, many cosmetics use traditional oils and moisturizers such as glycerin, sodium hyaluronate, etc., which can supplement and lock skin moisture to a certain extent, but the effect on deep skin nourishment and improvement of skin barrier function is not ideal. Moreover, the formula of some products is not scientific, the synergy between different raw materials is poor, and it is difficult to achieve long-term moisturizing. In addition, some moisturizing products have an oily texture, affecting the use experience, especially not suitable for people with oily skin.

[0006] Therefore, it is of great practical significance to develop a cosmetic that can simultaneously achieve anti-wrinkle firming and moisturizing effects, and has a good use experience and high stability. It is urgent to provide a new anti-wrinkle firming and moisturizing cosmetic and its preparation raw materials to solve the above technical problems. SUMMARY

[0007] The application aims to provide an anti-wrinkle and skin-tightening and moisturizing cosmetic and a preparation method thereof, which can realize the anti-wrinkle and skin-tightening and moisturizing effects simultaneously, and has good use feeling and high stability.

[0008] The anti-wrinkle and skin-tightening and moisturizing cosmetic is prepared from the following raw materials in percentage by weight: 5.5%-6.5% of a composite polypeptide freeze-dried powder, 2%-3% of a plant extract, 7%-9% of a humectant, 2.5%-3.5% of an emulsifier, 10%-15% of a moisturizing agent, 0.1%-0.3% of a preservative, and water to make up the rest to 100%.

[0009] The preparation method of the composite polypeptide freeze-dried powder comprises the following steps: mixing an active polypeptide, a polypeptide carrier and a protective agent, stirring until completely dissolved, homogenizing, and freeze-drying to obtain the product.

[0010] Preferably, the active polypeptide is acetyl hexapeptide-8, palmitoyl tripeptide-1, snake venom peptide and acetyl octapeptide-3.

[0011] Preferably, the mass ratio of acetyl hexapeptide-8, palmitoyl tripeptide-1, snake venom peptide and acetyl octapeptide-3 is (2-4):(2-3):(1-2):1; more preferably, 6:5:3:2.

[0012] By selecting specific active polypeptides as raw materials of the anti-wrinkle and skin-tightening and moisturizing cosmetic, the moisturizing effect of the cosmetic can be improved while the anti-wrinkle effect is improved. This may be because several polypeptides work together to repair the dermal matrix while targeting multiple sites to reduce wrinkles. Acetyl hexapeptide-8 and acetyl octapeptide-3 work together to inhibit neurotransmitter release, reduce muscle contraction, and block nerve signals. Snake venom peptide and palmitoyl tripeptide-1, acetyl octapeptide-3 work together to first inhibit muscle contraction by simulating snake venom toxins, quickly reduce expression lines in the short term, then continuously stimulate collagen regeneration while inhibiting muscle contraction, and improve the anti-wrinkle effect through the combination of quick-acting and long-acting. Acetyl octapeptide-3 and palmitoyl tripeptide-1 can also reduce neurogenic inflammation while repairing the dermal matrix, thereby reducing transdermal water loss and improving moisturizing effect. However, these polypeptides have poor stability and are easily affected by temperature, pH, microorganisms and other factors in a liquid environment, thereby losing activity and affecting the efficacy of the cosmetic.

[0013] Preferably, the polypeptide carrier is a beta-glucan aqueous solution and a hyaluronic acid nanosphere.

[0014] Preferably, the mass ratio of the beta-glucan aqueous solution and the hyaluronic acid nanosphere is (4-6):1; more preferably, 5:1.

[0015] Preferably, the mass fraction of beta-glucan in the beta-glucan aqueous solution is 3%.

[0016] Preferably, the hyaluronic acid nanosphere has a particle size of 50-100 nm.

[0017] In some preferred embodiments, the hyaluronic acid nanosphere is from Nanjing Jikeli Biotechnology Co., Ltd.

[0018] Preferably, the protective agent comprises trehalose and mannitol.

[0019] Preferably, the mass ratio of the trehalose and mannitol is 1: (0.5-2); further preferably, 1:1.

[0020] Preferably, the mass ratio of the active polypeptide, polypeptide carrier and protective agent is 1: (1.5-2.5): (0.8-1.2).

[0021] The specific steps of the homogenization are as follows: temperature adjustment to 4℃, homogenization once at 500 bar for 3 min, and then homogenization twice at 800 bar, each for 2 min.

[0022] The specific steps of the lyophilization are as follows: temperature reduction to -50℃ at a rate of 1℃ / min, maintaining for 6 hours, adjustment of vacuum degree to 0.1 Pa, temperature increase to -20℃ at a rate of 0.3℃ / min, maintaining for 48 hours, and then temperature increase to 25℃ at a rate of 0.3℃ / min, maintaining for 36 hours.

[0023] By selecting a specific lyophilized powder preparation method, the active polypeptide is prepared into a complex polypeptide lyophilized powder, which can maximize the retention of the biological activity and stability of the polypeptide, thereby improving the efficacy of the cosmetic. This may be because, first, a specific polypeptide carrier is selected to improve the permeability and stability of the polypeptide, reduce the irritation to the skin, and form a glassy protective layer to prevent the denaturation of the polypeptide during the lyophilization process. Finally, through gradient pressure homogenization and gradient lyophilization, the polypeptide is ensured to be uniformly dispersed, and the biological activity of the polypeptide is maximized.

[0024] Preferably, the plant extract is an extract of Petroselinum segetum, Centella asiatica and Panax ginseng. ERYNGIUM MARITIMUM ) extract, Centella asiatica extract and Panax ginseng extract.

[0025] Preferably, the mass ratio of the Petroselinum segetum extract, Centella asiatica extract and Panax ginseng extract is 1: (1.3-1.8): (1.5-2.5); further preferably, 2:3:4.

[0026] By selecting the coastal oenanthe stolonifera extract, centella asiatica extract and ginseng extract as the plant extract, the effective components of each component are utilized, which not only can promote the regeneration of collagen and cells, but also can improve the antioxidant and anti-inflammatory effects of the cosmetic, thereby improving the protection effect and barrier repair function of the skin. This may be because the effective components in the three plants are extracted by a specific method, and these effective components have a synergistic effect. The coastal oenanthe stolonifera is rich in polyphenols and flavonoids, and its strong antioxidant capacity can neutralize the damage of free radicals to collagen fibers, providing the first line of defense against wrinkles; the triterpenoids in centella asiatica (such as hydroxyl asiatic acid) can reconstruct the dermal layer support structure by promoting fibroblast proliferation and collagen synthesis, and its anti-inflammatory properties can help alleviate skin aging caused by chronic micro-inflammation; the ginsenosides in ginseng extract not only activate the AMPK pathway to enhance cell metabolic activity, but also optimize the skin hydration state by up-regulating the expression of hyaluronic acid synthase gene. The three form a closed loop in the mechanism of action: the coastal oenanthe stolonifera removes free radicals and reduces collagen degradation, the centella asiatica accelerates collagen neogenesis and remodels the skin skeleton, and the ginseng strengthens cell metabolism and locks in moisture, the three work together to achieve the triple effects of reducing wrinkles, improving firmness and long-lasting moisturizing, and significantly enhance the various effects of the cosmetic through multi-dimensional and multi-target joint action. The preparation method of the coastal oenanthe stolonifera extract comprises the following steps:

[0027] A1, dry and crush the stems and leaves of coastal oenanthe stolonifera (for example, crush to a particle size of less than 100 μm), to obtain pretreated coastal oenanthe stolonifera;

[0028] A2, activate the starter culture to obtain an activated liquid; mix the pretreated coastal oenanthe stolonifera and glucose uniformly, add water to adjust the moisture content (for example, the moisture content is 45wt%-55wt%) to obtain a fermentation substrate, inoculate the activated liquid, and ferment at 25-40℃ for 40-100h to obtain a fermentation product;

[0029] A3, mix the fermentation product and deionized water, ultrasonic water extraction, centrifugation, the supernatant is passed through an ultrafiltration membrane to obtain a macromolecular protein with a molecular weight of >10kDa, dialysis with pure water, and spray drying to obtain the product.

[0030] Preferably, the mass ratio of the stems and leaves is 1:2.

[0031] Preferably, the addition amount of glucose is 2%-4% of the mass of the pretreated coastal oenanthe stolonifera.

[0032] Preferably, the starter culture is lactobacillus plantarum.

[0033] The lactobacillus plantarum has a strain number CICC 24936.

[0034] The preparation method of the activation solution comprises the following steps: grafting Lactobacillus plantarum in MRS culture medium, and culturing the Lactobacillus plantarum in an anaerobic tank at 37 DEG C for 24 hours to ensure that the viable bacterial count of the bacterial body collected by centrifugation is greater than or equal to 1x10 10 CFU / mL, and the activation solution is obtained.

[0035] Preferably, the Lactobacillus plantarum is used in an amount of 10 8 -10 9 CFU / g of fermentation substrate.

[0036] Preferably, the mass ratio of the fermentation product to deionized water is 1: (7-9).

[0037] The specific conditions of the ultrasonic water extraction are as follows: the frequency is 40 kHz, the power is 500 W, the temperature is 45 DEG C, the time is 30 min, the mode is pulse mode, and the ultrasonic is turned off for 2 s every 5 s of working.

[0038] The preparation method of the Centella asiatica extract comprises the following steps:

[0039] B1, washing and drying Centella asiatica leaves to obtain Centella asiatica powder, adding water to adjust the water content to 40%-50%, adjusting the temperature to 45-50 DEG C, adjusting the pH to 4.5-5.5, adding cellulase, and carrying out enzymolysis for 2-3 hours and then boiling and inactivating to obtain Centella asiatica enzymolysis product;

[0040] B2, adding 80 DEG C hot water to the Centella asiatica enzymolysis product for 2 times of extraction, each time for 1-2 hours, combining the filtrates, reducing pressure and concentrating, and drying until the water content is less than or equal to 0.1% to obtain the Centella asiatica extract.

[0041] Preferably, the cellulase is added in an amount of 80-120 U / g of the Centella asiatica powder.

[0042] Preferably, the mass ratio of the Centella asiatica enzymolysis product to 80 DEG C hot water is 1: (10-20).

[0043] The preparation method of the ginseng extract comprises the following steps:

[0044] C1, washing and drying ginseng roots to obtain ginseng root powder, refluxing and extracting the ginseng root powder with 70% ethanol aqueous solution for 3 times, combining the extraction liquid, concentrating the extraction liquid to 25% of the volume of the extraction liquid to obtain a concentrated liquid, adding 3 times the volume of 95% ethanol aqueous solution, and standing at 4 DEG C for more than 12 hours to obtain a precipitate and a supernatant by centrifugation;

[0045] C2, carrying out supercritical carbon dioxide extraction on the supernatant and then rotary evaporation to obtain a solid;

[0046] C3, mixing the precipitate and the solid to obtain the ginseng extract.

[0047] Preferably, the mass ratio of the ginseng root powder and the 70% ethanol aqueous solution is 1: (10-20).

[0048] Preferably, the specific conditions of the supercritical carbon dioxide extraction are as follows: the pressure is 23-28 MPa, the carbon dioxide flow rate is 18-22 L / h, the entrainer flow rate is 0.5-1.5 mL / min, the extraction time is 3.5-4.5 h, and the extraction temperature is 43-48℃.

[0049] Preferably, the entrainer is a 65%-75% ethanol aqueous solution.

[0050] By targeting the effective components of the three plants, and adopting specific lactic acid bacteria fermentation, enzymatic hydrolysis, supercritical carbon dioxide extraction and other processes, the bound flavonoids in the coastal Osmorhiza japonica are converted into free state, the bioavailability is improved; the cell wall is destroyed by enzymatic hydrolysis, the yield of triterpenoid compounds is improved; the supercritical carbon dioxide extraction selectively enriches small molecule saponins such as Rg3, greatly improving the content of effective components in each extract, thereby improving the efficacy in cosmetics.

[0051] Preferably, the humectant is a hyaluronic acid complex, sodium polyglutamate and glycerol.

[0052] Preferably, the mass ratio of the hyaluronic acid complex, sodium polyglutamate and glycerol is (1-2):1:(1-2); further preferably, 3:2:3.

[0053] The preparation method of the hyaluronic acid complex comprises the following steps: adding deionized water to macromolecular hyaluronic acid, medium molecular hyaluronic acid and small molecular hyaluronic acid to prepare macromolecular hyaluronic acid gel, medium molecular hyaluronic acid gel and small molecular hyaluronic acid gel, respectively; then pouring the medium molecular hyaluronic acid gel into the small molecular hyaluronic acid gel and homogenizing (for example, homogenizing at 800 bar for 3 min), pouring into the macromolecular hyaluronic acid gel and homogenizing (for example, homogenizing at 500 bar for 5 min) to obtain a complex gel; and freeze-drying the complex gel to remove the deionized water to obtain the hyaluronic acid complex. The temperature is controlled below 20℃ during homogenization.

[0054] Preferably, the molecular weight of the macromolecular hyaluronic acid is 1500k-2500kDa; the molecular weight of the medium molecular hyaluronic acid is 40k-100kDa; and the molecular weight of the small molecular hyaluronic acid is 1k-10kDa.

[0055] In some preferred embodiments, the macromolecular hyaluronic acid and the medium molecular hyaluronic acid are both from Shanghai Maikelin Biochemical Technology Co., Ltd.; and the small molecular hyaluronic acid is from Blue Force Biotech (Xi'an) Co., Ltd.

[0056] Preferably, the mass ratio of the macromolecular hyaluronic acid, the medium molecular hyaluronic acid and the small molecular hyaluronic acid is (1-1.5):(1.3-1.8):1; more preferably, 5:6:4.

[0057] By selecting different molecular weights of hyaluronic acid, the moisturizing property of the cosmetic can be improved. This may be because the macromolecular hyaluronic acid forms a water-locking film, reduces transdermal water loss, the medium molecular hyaluronic acid penetrates into the dermis layer, promotes the proliferation of basal cells, and the small molecular hyaluronic acid activates aquaporin, improves the water absorption capacity of cells. The three molecular weights of hyaluronic acid synergistically act to realize deep moisturizing through the triple action of macromolecular water locking, medium molecular penetration and small molecular activation, greatly improving the water content of the stratum corneum. Moreover, the medium molecular hyaluronic acid penetrating into the dermis layer can also stimulate the proliferation of fibroblasts, and synergistically promote the generation of collagen with polypeptides. However, directly mixing the three molecular weights of hyaluronic acid will cause the macromolecular hyaluronic acid to form a viscous layer on the surface of the solution, which will wrap the medium molecular and small molecular hyaluronic acid, hindering their penetration into the deep layer, thereby affecting the moisturizing effect. Moreover, the charge density and intermolecular force of different molecular weights of hyaluronic acid are quite different, and direct mixing may cause crosslinking or precipitation, affecting the stability of the skin care product.

[0058] Preferably, the mass fraction of the macromolecular hyaluronic acid, the medium molecular hyaluronic acid and the small molecular hyaluronic acid in the macromolecular hyaluronic acid gel, the medium molecular hyaluronic acid gel and the small molecular hyaluronic acid gel is 3%-5%.

[0059] The preparation method of the macromolecular hyaluronic acid gel comprises the following steps: adding deionized water at 80℃ into the macromolecular hyaluronic acid, and stirring at 300 rpm for 2 h after standing at room temperature for 30-50 min.

[0060] The preparation method of the medium molecular hyaluronic acid gel comprises the following steps: adding deionized water at 50℃ into the medium molecular hyaluronic acid, and stirring at 300 rpm for 1 h at 50℃.

[0061] The preparation method of the small molecular hyaluronic acid gel comprises the following steps: adding deionized water at 25℃ into the small molecular hyaluronic acid, and stirring at 2500 rpm for 5-10 min.

[0062] The specific steps of the freeze-drying are as follows: cooling at a rate of 1℃ / min to -50℃, maintaining for 6 hours, adjusting the vacuum degree to 0.1 Pa, warming to -20℃ at a rate of 0.3℃ / min, maintaining for 48 hours, and then warming to 25℃ at a rate of 0.3℃ / min until the mass no longer decreases.

[0063] By preparing hyaluronic acid gels of different molecular weights and mixing them in a gradient from small to large, the small molecules are not wrapped by large molecules, the stability of the system is enhanced, a biomimetic layered structure is formed, the rate of transdermal water loss is reduced, and the synergistic effect with the other two moisturizing agents is achieved. The negative charge of polyglutamic acid sodium forms an electrostatic grid with hyaluronic acid, crosslinks into a film, reduces water evaporation, and plays a barrier repair role, greatly improves the water content of the stratum corneum, and prolongs the moisturizing time. Glycerin not only has an instant moisturizing effect, but also reduces the sticky feeling of hyaluronic acid and improves the comfort of use.

[0064] Preferably, the molecular weight of the polyglutamic acid sodium is ≤100kDa.

[0065] In some preferred embodiments, the polyglutamic acid sodium is from Shanghai Maikelin Biochemical Technology Co., Ltd.

[0066] Preferably, the emulsifier includes PEG-7 olive oil acid ester.

[0067] Preferably, the HLB value of the PEG-7 olive oil acid ester is 11, and the hydroxyl value is 170-185mg KOH / g.

[0068] In some preferred embodiments, the PEG-7 olive oil acid ester is from Wuhan Huaxiang Kejebio Technology Co., Ltd.

[0069] Preferably, the emollient includes caprylic acid / capric acid glycerin triester, white pool seed oil, and camellia oil nano-liposome, with a mass ratio of (1.5-2):(1-1.5):1; further preferably, 5:4:3.

[0070] Using caprylic / capric triglycerides, meadowfoam seed oil, and camellia oil as emollients not only enhances the anti-wrinkle and firming properties of cosmetics but also improves their moisturizing effect and reduces transepidermal water loss. This is likely due to the synergistic effect of the three: meadowfoam seed oil provides ceramide analogs such as meadowfoam oligolactone, while camellia oil replenishes ω-3 / ω-6 fatty acids, thus supplementing lipid components; caprylic / capric triglycerides act as a "liquid scaffold," filling the gaps in the lamellar structure of the stratum corneum; simultaneously, squalene in camellia oil activates β-glucocerebrosidase, accelerating barrier lipid synthesis. The synergistic effect of these three ingredients completes the barrier repair matrix, thereby improving moisturizing effects and reducing transepidermal water loss. On the other hand, meadowfoam seed oil inhibits matrix metalloproteinase-1 activity, reduces collagen degradation, and protects collagen in the skin; camellia oil's linolenic acid is converted into prostaglandin E2, upregulating the expression of transforming growth factor and promoting the generation of new cells; caprylic / capric triglycerides form an elastic film on the skin surface, providing an immediate firming sensation. The combination of these three ingredients forms a dual pathway for anti-wrinkle and firming, effectively reducing the area and depth of fine lines. However, camellia oil is easily oxidized, resulting in poor system stability. Furthermore, to achieve better moisturizing and anti-wrinkle effects, the oleic acid in camellia oil needs to penetrate the stratum corneum to stimulate fibroblasts.

[0071] The method for preparing the camellia oil nanoliposomes includes the following steps: mixing camellia oil, hydrogenated lecithin, cholesterol, co-solvent, and stabilizer, ultrasonically emulsifying for 5 minutes, placing it in a high-pressure reactor for supercritical carbon dioxide treatment, and homogenizing after depressurization (e.g., homogenizing for 5 minutes at a pressure of 80 bar and a temperature of 15°C) to obtain the final product.

[0072] Preferably, the co-solvent is an aqueous ethanol solution with a mass fraction of 95%.

[0073] Preferably, the stabilizer is a mixed aqueous solution of poloxamer 188 and trehalose, wherein the mass fraction of poloxamer 188 is 3% and the mass fraction of trehalose is 5%.

[0074] Preferably, the mass ratio of camellia oil, hydrogenated lecithin, and cholesterol is (25-35):(4-6):1; more preferably, it is 30:5:1.

[0075] Preferably, the amount of the co-solvent added is 10%-15% of the total mass of camellia oil, hydrogenated lecithin, and cholesterol.

[0076] Preferably, the amount of stabilizer added is 2.5-3 times the total mass of camellia oil, hydrogenated lecithin, and cholesterol.

[0077] Preferably, the specific conditions for ultrasonic emulsification are: frequency of 40kHz, power of 150W, and temperature of 25℃.

[0078] Preferably, the supercritical carbon dioxide treatment is performed at a pressure of 20 MPa, a temperature of 45℃, and for 30 min.

[0079] By selecting hydrogenated lecithin and cholesterol to construct the nanoliposome skeleton of camellia oil, not only can the stability be improved, the active ingredients in the system can be protected, but also the utilization efficiency in skin care can be significantly improved. This may be because there is a synergistic effect between hydrogenated lecithin and cholesterol. As the core builder of the nanoliposome, hydrogenated lecithin can spontaneously form nanoscale vesicles in the film dispersion or emulsification process due to its unique amphiphilic structure. These vesicles not only tightly wrap the active ingredients such as polyphenols and squalene in camellia oil, preventing them from being oxidized or degraded during storage or application, but also promote the penetration of camellia oil to the skin basal layer by efficiently fusing with the skin cell membrane due to its strong skin affinity. At the same time, cholesterol, as a key regulator of the stability of the nanoliposome, precisely embeds into the phospholipid bilayer, limiting the excessive fluidity of the membrane, and on the macro level, it builds a solid barrier for the nanoliposome vesicles, effectively resisting the destruction of external factors such as temperature changes and mechanical stress, ensuring the integrity of the nanoliposome and the long-term retention of active ingredients during the storage period. On the micro level, cholesterol and hydrogenated lecithin synergistically regulate the particle size distribution of the nanoliposome, making the vesicle size more uniform, thereby optimizing the spreading and absorption efficiency of camellia oil on the skin surface. Through dynamic functional complementation, they not only achieve multiple protection of the active ingredients in camellia oil, but also precisely control the release and absorption process in the time and space dimensions, ultimately significantly improving the utilization efficiency of camellia oil in skin care. In addition, the use of supercritical carbon dioxide method instead of traditional chloroform method to prepare camellia oil nanoliposomes not only has no solvent residue, is safe and non-toxic, and is suitable for the cosmetics field, but also has stable encapsulation efficiency and narrow particle size distribution. However, the solubility of camellia oil in the supercritical carbon dioxide system is low, so a certain amount of cosolvent and stabilizer needs to be added to improve the solubility of the lipids and the stability, thereby improving the encapsulation efficiency and the moisturizing effect of the emollient and cosmetic.

[0080] In some preferred embodiments, the white pool seed oil and camellia oil are both from Jiangxi Zhonghuan Biotechnology Co., Ltd.

[0081] Preferably, the preservative includes 1,2-hexanediol and p-hydroxyacetophenone at a mass ratio of 5:3.

[0082] The preparation method of the anti-wrinkle firming and moisturizing cosmetic comprises the following steps:

[0083] S1, water phase preparation: mix the humectant and water, stir until completely dissolved to obtain the water phase;

[0084] S2, oil phase preparation: after mixing the emollients, the emulsifiers are added and stirred uniformly (for example, stirring at 1000 rpm for 10 min to uniformity), to obtain the oil phase;

[0085] S3, homogenization emulsification: the water phase and the oil phase are heated (for example, heated to 45 DEG C) respectively, the oil phase is poured into the water phase, and the homogenizer is processed (for example, processed for 5 min under a pressure of 800 bar), cooled (for example, cooled to 30 DEG C), the composite polypeptide freeze-dried powder and the plant extract are added, stirred uniformly, the preservatives are added, and continue to be stirred uniformly, and vacuum degassing is performed, to obtain the product.

[0086] Compared with the prior art, the advantages and beneficial effects of the present application are:

[0087] 1. The anti-wrinkle firming and emollient type cosmetic prepared by the present application can realize the anti-wrinkle firming and emollient effects, and has good use feeling and high stability.

[0088] 2. The anti-wrinkle firming and emollient type cosmetic prepared by the present application can improve the moisturizing effect while improving the anti-wrinkle effect, and the active polypeptide is prepared into a composite polypeptide freeze-dried powder by a specific freeze-dried powder preparation method, so that the biological activity and stability of the polypeptide are maximally retained, thereby improving the efficacy of the cosmetic.

[0089] 3. The anti-wrinkle firming and emollient type cosmetic prepared by the present application uses the effective components of the coastal oyster plant extract, centella asiatica extract and ginseng extract, which can promote the regeneration of collagen and cells, improve the antioxidant and anti-inflammatory effects of the cosmetic, and improve the protection effect and barrier repair function of the skin.

[0090] 4. The anti-wrinkle firming and emollient type cosmetic prepared by the present application uses hyaluronic acid of different molecular weights, which are prepared into hyaluronic acid gels, and then mixed in a gradient from small to large, so that the moisturizing property and the system stability of the cosmetic are improved.

[0091] 5. The anti-wrinkle firming and emollient type cosmetic prepared by the present application uses caprylic / capric triglyceride, white pool flower seed oil and camellia oil as emollients, which can improve the anti-wrinkle firming property and the moisturizing property of the cosmetic, and reduce the trans-epidermal water loss rate.

[0092] 6. The anti-wrinkle firming and emollient type cosmetic prepared by the present application uses hydrogenated phospholipids and cholesterol to construct the camellia oil nanoliposome skeleton, which can improve the stability, protect the active ingredients in the system, and significantly improve the utilization efficiency in skin care. DETAILED DESCRIPTION

[0093] The technical solutions in the embodiments of the present application will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the protection scope of the present application.

[0094] The raw materials used in the present application are commercially available, and specifically are as follows:

[0095] Hyaluronic acid nanospheres with a particle size of 50-100 nm are from Nanjing Gike Biological Technology Co., Ltd.

[0096] Lactobacillus plantarum with strain number CICC 24936 is from China Industrial Microbial Culture Collection Center.

[0097] MRS medium is from Guangdong Huan Kai Microbial Technology Co., Ltd.

[0098] Cellulase with an enzyme activity of about 11000 U / g is from Ningxia Xiasen Industry Group Co., Ltd.

[0099] Macromolecular hyaluronic acid with a molecular weight of 1500 k-2500 kDa and medium molecular weight hyaluronic acid with a molecular weight of 40 k-100 kDa are both from Shanghai Macklin Biochemical Technology Co., Ltd.

[0100] Small molecular hyaluronic acid with a molecular weight of 1 k-10 kDa is from Blue Force Biotech (Xi'an) Co., Ltd.

[0101] Sodium polyglutamate with a molecular weight of less than 100 kDa is from Shanghai Macklin Biochemical Technology Co., Ltd.

[0102] PEG-7 olive oil acid ester with an HLB value of 11 and a hydroxyl value of 170-185 mg KOH / g is from Wuhan Huaxiang Kejebio Technology Co., Ltd.

[0103] White pool flower seed oil and camellia oil are both from Jiangxi Zhonghuan Biological Technology Co., Ltd.

[0104] Embodiment 1

[0105] The present embodiment provides an anti-wrinkle firming and moisturizing type cosmetic, and the preparation raw materials thereof are as follows in terms of percentage by weight: 6.0% of composite polypeptide freeze-dried powder, 2.5% of plant extract, 8% of humectant, 3% of emulsifier, 12.5% of emollient, 0.2% of preservative, and water supplementing the balance to 100%.

[0106] The preparation method of the composite polypeptide freeze-dried powder comprises the following steps: mixing the active polypeptide, the polypeptide carrier and the protective agent, stirring until completely dissolved, homogenizing, and freeze-drying.

[0107] The active polypeptide is acetyl hexapeptide-8, palmitoyl tripeptide-1, snake venom peptide, and acetyl octapeptide-3, with a mass ratio of 6:5:3:2.

[0108] The polypeptide carrier is a beta-glucan aqueous solution and a hyaluronic acid nanosphere, with a mass ratio of 5:1.

[0109] The mass fraction of beta-glucan in the beta-glucan aqueous solution is 3%.

[0110] The protective agent is trehalose and mannitol, with a mass ratio of 1:1.

[0111] The mass ratio of the active polypeptide, the polypeptide carrier and the protective agent is 1:2:1.

[0112] The specific homogenization steps are as follows: adjusting the temperature to 4 DEG C, homogenizing once at a pressure of 500 bar for 3 min, and then homogenizing twice at 800 bar, each for 2 min.

[0113] The specific freeze-drying steps are as follows: reducing the temperature to -50 DEG C at a rate of 1 DEG C / min, maintaining for 6 h, adjusting the vacuum degree to 0.1 Pa, increasing the temperature to -20 DEG C at a rate of 0.3 DEG C / min, maintaining for 48 h, then increasing the temperature to 25 DEG C at a rate of 0.3 DEG C / min, and maintaining for 36 h.

[0114] The plant extract is a coastal Eupatorium fortunei extract, a Centella asiatica extract and a ginseng extract, with a mass ratio of 2:3:4.

[0115] The preparation method of the coastal Eupatorium fortunei extract comprises the following steps:

[0116] A1, drying and crushing stems and leaves of the coastal Eupatorium fortunei to a particle size of less than 100 mu m to obtain pretreated coastal Eupatorium fortunei;

[0117] A2, activating a fermentation agent to obtain an activated liquid; mixing the pretreated coastal Eupatorium fortunei and glucose uniformly, adding water to adjust the water content to 50 wt%, and inoculating the activated liquid to ferment at 28 DEG C for 55 h to obtain a fermentation product;

[0118] A3, mixing the fermentation product and deionized water, ultrasonic water extraction, centrifugation, passing the supernatant through an ultrafiltration membrane to obtain macromolecular protein with a molecular weight of > 10 kDa, dialysis with pure water for 36 h, and spray drying to a water content of 0.05% to obtain the product.

[0119] The mass ratio of the stems and leaves is 1:2.

[0120] The added amount of glucose is 3% of the pretreated sea parsnip mass.

[0121] The preparation method of the activation solution comprises the following steps: grafting Lactobacillus plantarum in MRS culture medium, and culturing in an anaerobic tank at 37℃ for 24 hours to ensure that the viable bacterial count of the bacteria collected by centrifugation is 1×10 10 CFU / mL, thereby obtaining the activation solution.

[0122] The dosage of the Lactobacillus plantarum is 10 9 CFU / g of fermentation substrate.

[0123] The mass ratio of the fermentation product to deionized water is 1:8.

[0124] The specific conditions of the ultrasonic water extraction are as follows: the frequency is 40 kHz, the power is 500 W, the temperature is 45℃, the time is 30 min, the mode is pulse mode, and the ultrasonic is turned off for 2 s every 5 s of working.

[0125] The preparation method of the Centella asiatica extract comprises the following steps:

[0126] B1, washing and drying Centella asiatica leaves to obtain Centella asiatica powder, adding water to adjust the water content to 45%, adjusting the temperature to 48℃, adjusting the pH to 5, adding cellulase, and enzyme hydrolyzing for 2.5 hours and then boiling and inactivating, thereby obtaining Centella asiatica enzyme hydrolysate;

[0127] B2, adding 80℃ hot water to the Centella asiatica enzyme hydrolysate for 2 times of extraction, each time for 1.5 hours, combining the filtrates, reducing pressure and concentrating, and then drying to a water content of 0.05%, thereby obtaining the Centella asiatica extract.

[0128] The added amount of the cellulase is 100 U / g of the Centella asiatica powder.

[0129] The mass ratio of the Centella asiatica enzyme hydrolysate to 80℃ hot water is 1:15.

[0130] The preparation method of the ginseng extract comprises the following steps:

[0131] C1, washing and drying ginseng roots to obtain ginseng root powder, refluxing and extracting the ginseng root powder with 70% ethanol aqueous solution for 3 times, combining the extraction liquid, concentrating the extraction liquid to 25% of the volume of the extraction liquid to obtain a concentrated liquid, adding 3 times the volume of the concentrated liquid of 95% ethanol aqueous solution, and standing at 4℃ for 12 hours to obtain a precipitate and a supernatant by centrifugation;

[0132] C2, performing supercritical carbon dioxide extraction on the supernatant and then rotary evaporation, thereby obtaining a solid;

[0133] C3, mixing the precipitate and the solid, thereby obtaining the ginseng extract.

[0134] The mass ratio of the ginseng root powder and the 70% ethanol aqueous solution is 1:15.

[0135] The specific conditions of the supercritical carbon dioxide extraction are: pressure 25 MPa, carbon dioxide flow rate 20 L / h, entrainer flow rate 1 mL / min, extraction time 4 h, and extraction temperature 45℃.

[0136] The entrainer is a 70% ethanol aqueous solution.

[0137] The moisturizing agent is a hyaluronic acid compound, sodium polyglutamate, and glycerol, and the mass ratio is 3:2:3.

[0138] The preparation method of the hyaluronic acid compound comprises the following steps: adding deionized water into macromolecular hyaluronic acid, medium molecular hyaluronic acid, and small molecular hyaluronic acid to prepare macromolecular hyaluronic acid gel, medium molecular hyaluronic acid gel, and small molecular hyaluronic acid gel; pouring the medium molecular hyaluronic acid gel into the small molecular hyaluronic acid gel, homogenizing at 800 bar for 3 min, and then pouring into the macromolecular hyaluronic acid gel, homogenizing at 500 bar for 5 min to obtain a compound gel; and controlling the temperature below 20℃ during homogenization, and freeze-drying the compound gel to remove the deionized water.

[0139] The mass ratio of the macromolecular hyaluronic acid, the medium molecular hyaluronic acid, and the small molecular hyaluronic acid is 5:6:4.

[0140] The mass fraction of the macromolecular hyaluronic acid, the medium molecular hyaluronic acid, and the small molecular hyaluronic acid in the macromolecular hyaluronic acid gel, the medium molecular hyaluronic acid gel, and the small molecular hyaluronic acid gel is 4%.

[0141] The preparation method of the macromolecular hyaluronic acid gel comprises the following steps: adding 80℃ deionized water into the macromolecular hyaluronic acid, and stirring at 300 rpm for 2 h after standing at room temperature for 40 min.

[0142] The preparation method of the medium molecular hyaluronic acid gel comprises the following steps: adding 50℃ deionized water into the medium molecular hyaluronic acid, and stirring at 300 rpm for 1 h at 50℃.

[0143] The preparation method of the small molecular hyaluronic acid gel comprises the following steps: adding 25℃ deionized water into the small molecular hyaluronic acid, and stirring at 2500 rpm for 8 min.

[0144] The specific steps of the freeze-drying are as follows: reducing the temperature to-50℃ at a rate of 1℃ / min, maintaining for 6 hours, adjusting the vacuum degree to 0.1 Pa, increasing the temperature to-20℃ at a rate of 0.3℃ / min, maintaining for 48 hours, and then increasing the temperature to 25℃ at a rate of 0.3℃ / min until the mass no longer decreases.

[0145] The emulsifier is PEG-7 olive oil ester.

[0146] The emollient includes caprylic / capric triglyceride, white pool seed oil, camellia oil nanoliposome, and the mass ratio is 5:4:3.

[0147] The preparation method of the camellia oil nanoliposome includes the following steps: mixing camellia oil, hydrogenated lecithin, cholesterol, a cosolvent, and a stabilizer, ultrasonic emulsification for 5 min, and then placing in a high-pressure reaction kettle for supercritical carbon dioxide treatment, and then homogenizing at a pressure of 80 bar and a temperature of 15℃ for 5 min.

[0148] The cosolvent is a 95% mass fraction ethanol aqueous solution.

[0149] The stabilizer is a mixed aqueous solution of poloxamer 188 and trehalose, wherein the mass fraction of poloxamer 188 is 3% and the mass fraction of trehalose is 5%.

[0150] The mass ratio of the camellia oil, hydrogenated lecithin, and cholesterol is 30:5:1.

[0151] The addition amount of the cosolvent is 12% of the total mass of the camellia oil, hydrogenated lecithin, and cholesterol.

[0152] The addition amount of the stabilizer is 2.8 times the total mass of the camellia oil, hydrogenated lecithin, and cholesterol.

[0153] The specific conditions of the ultrasonic emulsification are as follows: a frequency of 40 kHz, a power of 150 W, and a temperature of 25℃.

[0154] The conditions of the supercritical carbon dioxide treatment are as follows: a pressure of 20 MPa, a temperature of 45℃, and a time of 30 min.

[0155] The preservative is 1,2-hexanediol and p-hydroxyacetophenone, and the mass ratio is 5:3.

[0156] The preparation method of the anti-wrinkle firming and emollient cosmetic includes the following steps:

[0157] S1, water phase preparation: mixing the humectant and water, and stirring until completely dissolved to obtain a water phase;

[0158] S2, oil phase preparation: mixing the emollient, adding the emulsifier, and stirring at 1000 rpm for 10 min until uniform to obtain an oil phase;

[0159] S3, homogenization and emulsification: the water phase and the oil phase were heated to 45℃ respectively, then the oil phase was slowly poured into the water phase, and the homogenizer was processed under a pressure of 800 bar for 5 min, cooled to 30℃, then the freeze-dried powder of the complex polypeptide and the plant extract were added, stirred uniformly, then the preservative was added, and stirred uniformly, vacuum degassing, and the product was obtained.

[0160] Example 2

[0161] The difference between this example and Example 1 is that the active polypeptide is acetyl hexapeptide-8, palmitoyl tripeptide-1, snake venom-like peptide, and acetyl octapeptide-3, with a mass ratio of 4:2:1:1.

[0162] Comparative Example 1

[0163] The difference between this comparative example and Example 1 is that the active polypeptide is acetyl hexapeptide-8, palmitoyl tripeptide-1, and acetyl octapeptide-3, with a mass ratio of 6:5:2.

[0164] Comparative Example 2

[0165] The difference between this comparative example and Example 1 is that the anti-wrinkle firming and moisturizing cosmetic is prepared from raw materials in the following proportions by weight: active polypeptide 6.0%, plant extract 2.5%, humectant 8%, emulsifier 3%, emollient 12.5%, preservative 0.2%, and water to make up the balance to 100%.

[0166] The active polypeptide is acetyl hexapeptide-8, palmitoyl tripeptide-1, snake venom-like peptide, and acetyl octapeptide-3, with a mass ratio of 6:5:3:2.

[0167] Comparative Example 3

[0168] The difference between this comparative example and Example 1 is that the plant extract is a coastal oenanthe javorica extract.

[0169] Comparative Example 4

[0170] The difference between this comparative example and Example 1 is that the humectant is a mixture of medium molecular weight hyaluronic acid, sodium polyglutamate, and glycerol, with a mass ratio of 3:2:3.

[0171] Comparative Example 5

[0172] The difference between this comparative example and Example 1 is that the preparation method of the hyaluronic acid complex is as follows: macromolecular hyaluronic acid, medium molecular weight hyaluronic acid, small molecular weight hyaluronic acid, and deionized water are mixed, homogenized at 500 bar for 5 min, and then rotary evaporated to remove the deionized water, and the product is obtained.

[0173] The mass ratio of the macromolecular hyaluronic acid, medium molecular weight hyaluronic acid, and small molecular weight hyaluronic acid is 5:6:4.

[0174] The adding amount of the deionized water is 10 times of the total mass of the macromolecular hyaluronic acid, the medium molecular hyaluronic acid and the small molecular hyaluronic acid.

[0175] Comparative Example 6

[0176] The difference between the present comparative example and Example 1 is that the emollient comprises caprylic / capric triglyceride, white pool seed oil and camellia oil at a mass ratio of 5:4:3.

[0177] Comparative Example 7

[0178] The difference between the present comparative example and Example 1 is that the preparation method of the camellia oil nano-liposome is as follows: the camellia oil, hydrogenated lecithin, co-solvent and stabilizer are mixed, ultrasonic emulsification is performed for 5 minutes, the mixture is put into a high-pressure reaction kettle for supercritical carbon dioxide treatment, and then homogenization is performed at 80 bar after pressure relief.

[0179] The mass ratio of the camellia oil to the hydrogenated lecithin is 5:1.

[0180] Performance test

[0181] ①Cosmetic stability test: The cosmetic is subjected to high temperature, low temperature and centrifugal test according to the Cosmetic Safety Technical Specification (2022 edition) to judge the stability of the cosmetic. High temperature test: the cosmetic sample is placed in a (40±1) ℃ constant temperature box for 30 days, and whether it is stratified, discolored or precipitated is observed. If not, it is qualified. Low temperature test: the cosmetic sample is frozen at (-15±1) ℃ for 24 hours, and then observed whether it is stratified, discolored or precipitated after recovery to room temperature. If not, it is qualified. Centrifugal test: the cosmetic sample is centrifuged at 3000 r / min for 30 minutes, and whether it is stratified or precipitated is checked. If not, it is qualified. The results are shown in Table 1.

[0182] Table 1 Test results

[0183]

[0184] According to statistics, the anti-wrinkle firming and emollient type cosmetic prepared in Examples 1-2 is qualified in high and low temperature and centrifugal tests, indicating that the cosmetic prepared by the present application has excellent stability. Comparative Example 1 does not add the snake venom-like peptide, Comparative Example 2 does not freeze-dry the active polypeptide, Comparative Example 3 does not add the extract of Gynostemma pentaphyllum and the extract of Panax ginseng, Comparative Example 4 uses medium molecular hyaluronic acid instead of the hyaluronic acid complex, Comparative Example 5 directly mixes three molecular weights of hyaluronic acid to prepare the hyaluronic acid complex, and Comparative Example 6 does not perform nano-liposome treatment on the camellia oil, and Comparative Example 7 does not add cholesterol, and each cosmetic has defects in stability.

[0185] ②Efficacy test

[0186] Select 90 female volunteers aged 25-55 years old, with rough and dull skin, lack of elasticity, with crow's feet and under-eye lines, and no skin diseases. The volunteers were divided into 9 groups, and the samples of Examples 1-2 and Comparative Examples 1-7 were used respectively, once in the morning and once in the evening according to the daily habits. After 1 month of trial, the transepidermal water loss rate of the volunteers before and after the trial was tested using a transepidermal water loss rate tester, and the transepidermal water loss rate change rate = (transepidermal water loss rate after trial - transepidermal water loss rate before trial) / transepidermal water loss rate before trial x 100%. Since the transepidermal water loss rate after the trial is smaller, the general change rate is negative, and the more negative the value, the better the moisturizing effect, the less the transepidermal water loss rate, and the average value is taken, and the results are shown in Table 2.

[0187] The 3D image of the local skin of the corner of the eye of the subject was taken by Primos CR, and the area of the crow's feet was analyzed by professional analysis software. After 1 month of trial, the area change rate of the crow's feet = (area of the crow's feet after trial - area of the crow's feet before trial) / area of the crow's feet before trial x 100%. Since the area of the crow's feet after the trial is smaller, the general change rate is negative, and the more negative the value, the better the anti-wrinkle effect, the less the skin wrinkles, and the average value is taken, and the results are shown in Table 2.

[0188] Table 2 Determination results

[0189]

[0190] According to statistics, the anti-wrinkle firming and moisturizing cosmetic prepared by Examples 1-2 has negative values of transepidermal water loss rate change rate and crow's feet area change rate, and the absolute values are high, indicating that the skin wrinkles are reduced and the moisturizing performance is increased after using the cosmetic. Comparative Example 1 does not add the snake venom-like peptide, Comparative Example 2 does not freeze-dry the active polypeptide, Comparative Example 3 does not add the extract of Gynostemma pentaphyllum and the extract of Panax ginseng, Comparative Example 4 uses medium molecular weight hyaluronic acid instead of hyaluronic acid complex, Comparative Example 5 directly mixes three molecular weights of hyaluronic acid to prepare the hyaluronic acid complex, and Comparative Example 6 does not perform nanoliposome treatment on the camellia oil, and Comparative Example 7 does not add cholesterol. The anti-wrinkle effect and moisturizing effect of the prepared cosmetic are poor after the trial, but still better than before the trial. Therefore, the cosmetic prepared by the raw materials and method described in the present application can not only achieve the effects of anti-wrinkle firming and moisturizing, but also has good use feeling and high stability.

[0191] The above is a preferred embodiment of the present application, and it should be noted that those skilled in the art can make several improvements and refinements without departing from the principles of the present application, and these improvements and refinements should also be considered within the scope of protection of the present application.

Claims

1. An anti-wrinkle firming, emollient cosmetic product, characterized in that, By weight percentage, its composition is: 5.5%-6.5% of complex polypeptide freeze-dried powder, 2%-3% of plant extract, 7%-9% of humectant, 2.5%-3.5% of emulsifier, 10%-15% of emollient, 0.1%-0.3% of preservative, and water makes up the rest to 100%; The preparation method of the complex polypeptide freeze-dried powder is prepared by the following steps: mixing active polypeptide, polypeptide carrier and protective agent, stirring until completely dissolved, homogenizing, freeze-drying, and the mass ratio of the active polypeptide, polypeptide carrier and protective agent is 1: (1.5-2.5): (0.8-1.2); the active polypeptide is acetyl hexapeptide-8, palmitoyl tripeptide-1, snake venom-like peptide and acetyl octapeptide-3, and the mass ratio is (2-4): (2-3): (1-2): 1; the polypeptide carrier is β-glucan aqueous solution and hyaluronic acid nanosphere, and the mass ratio is (4-6): 1, the particle size of the hyaluronic acid nanosphere is 50-100 nm, the protective agent includes trehalose and mannitol, and the mass ratio of the trehalose and mannitol is 1: (0.5-2), and the specific steps of freeze-drying are as follows: cooling to-50℃ at a rate of 1℃ / min, maintaining for 6 hours, adjusting the vacuum degree to 0.1Pa, warming to-20℃ at a rate of 0.3℃ / min, maintaining for 48 hours, then warming to 25℃ at a rate of 0.3℃ / min, and maintaining for 36 hours; The plant extract is a coastal Oenanthe javorica extract, a Centella asiatica extract and a ginseng extract, and the mass ratio is 1: (1.3-1.8): (1.5-2.5); The preparation method of the coastal Oenanthe javorica extract is as follows: A1, the stems and leaves of the coastal Oenanthe javorica are dried and then pulverized to a particle size of less than 100μm to obtain pretreated coastal Oenanthe javorica; A2、The fermentation agent is activated to obtain an activated liquid; the pretreated coastal parsnip and glucose are mixed uniformly, water is added to adjust to obtain a fermentation substrate with a water content of 45wt%-55wt%, and the fermentation substrate is inoculated with the activated liquid to ferment at 25-40 DEG C for 40-100h to obtain a fermentation product; the activated liquid is prepared by the following steps: grafting lactobacillus plantarum in MRS culture medium, and culturing in an anaerobic tank at 37 DEG C for 24h to ensure that the viable bacterial count of the bacteria collected by centrifugation is greater than or equal to 1x10 10 CFU / mL, and the activated liquid is obtained. A3, the fermentation product and deionized water are mixed, ultrasonic water extraction is performed, and then centrifugation is performed, the supernatant is passed through an ultrafiltration membrane to obtain macromolecular protein with a molecular weight of >10kDa, dialysis is performed with pure water, and then spray drying is performed to obtain the product; the specific conditions of the ultrasonic water extraction are as follows: the frequency is 40kHz, the power is 500W, the temperature is 45℃, the time is 30min, the mode is pulse mode, and the ultrasonic is turned off for 2s every 5s of work; The preparation method of the Centella asiatica extract is as follows: B1, the leaves of the Centella asiatica are washed, dried and pulverized to obtain Centella asiatica powder, water is added to adjust the water content to 40%-50%, the temperature is adjusted to 45-50℃, the pH is adjusted to 4.5-5.5, cellulase is added, and then enzyme hydrolysis is performed for 2-3h, and then boiling is performed for inactivation to obtain Centella asiatica hydrolysate; B2, hot water at 80℃ is added to the Centella asiatica hydrolysate for 2 times of extraction, each time for 1-2h, the filtrates are combined, concentrated under reduced pressure, and then dried until the water content is ≤0.1% to obtain the Centella asiatica extract; The preparation method of the ginseng extract is as follows: C1, after the ginseng root is cleaned, it is dried, pulverized to obtain ginseng root powder, and then extracted by refluxing with 70% ethanol aqueous solution for 3 times, and then the extract is combined, concentrated to 25% of the volume of the extract, and then 3 times the volume of 95% ethanol aqueous solution is added, and then the mixture is placed at 4℃ for 12 hours or more, and then centrifuged to obtain a precipitate and a supernatant; C2, the supernatant is extracted by supercritical carbon dioxide, and then rotary evaporated to obtain a solid; C3, the precipitate and the solid are mixed to obtain a ginseng extract; The humectant is a hyaluronic acid complex, sodium polyglutamate and glycerol, and the mass ratio of the hyaluronic acid complex, sodium polyglutamate and glycerol is (1-2):1:(1-2); the hyaluronic acid complex is prepared by the following method: adding deionized water to macromolecular hyaluronic acid, medium molecular hyaluronic acid and small molecular hyaluronic acid to prepare macromolecular hyaluronic acid gel, medium molecular hyaluronic acid gel and small molecular hyaluronic acid gel, then pouring the medium molecular hyaluronic acid gel into the small molecular hyaluronic acid gel and homogenizing, and then pouring into the macromolecular hyaluronic acid gel, and then homogenizing to obtain a composite gel, and then freeze-drying the composite gel to remove the deionized water to obtain the hyaluronic acid complex; the molecular weight of the macromolecular hyaluronic acid is 1500k-2500kDa; the molecular weight of the medium molecular hyaluronic acid is 40k-100kDa; the molecular weight of the small molecular hyaluronic acid is 1k-10kDa; the mass ratio of the macromolecular hyaluronic acid, the medium molecular hyaluronic acid and the small molecular hyaluronic acid is (1-1.5):(1.3-1.8):1; the mass fraction of the macromolecular hyaluronic acid, the medium molecular hyaluronic acid and the small molecular hyaluronic acid in the macromolecular hyaluronic acid gel, the medium molecular hyaluronic acid gel and the small molecular hyaluronic acid gel is 3%-5%; The emollient includes caprylic acid / capric acid triglyceride, white pool seed oil and camellia oil nanoliposomes, and the mass ratio is (1.5-2):(1-1.5):1; the camellia oil nanoliposomes are prepared by the following method: mixing camellia oil, hydrogenated lecithin, cholesterol, cosolvent and stabilizer, ultrasonic emulsification for 5 minutes, and then putting into a high-pressure reaction kettle for supercritical carbon dioxide treatment, and then homogenizing after pressure relief to obtain the camellia oil nanoliposomes, wherein the mass ratio of the camellia oil, the hydrogenated lecithin and the cholesterol is (25-35):(4-6):

1.

2. A method for preparing the anti-wrinkle firming, emollient cosmetic product according to Claim 1, characterized in that, The method comprises the following steps: S1, water phase preparation: mixing the humectant and water, stirring until completely dissolved to obtain a water phase; S2, oil phase preparation: mixing the emollient, adding the emulsifier, stirring uniformly to obtain an oil phase; S3, homogenization and emulsification: heating the water phase and the oil phase respectively, pouring the oil phase into the water phase, homogenizing in a homogenizer, cooling, adding the composite polypeptide freeze-dried powder and the plant extract, stirring uniformly, adding the preservative, continuing to stir uniformly, vacuum degassing to obtain the product.

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