Composition containing recombinant human albumin and acanthopanax senticosus extract

Abstract

The invention relates to the field of cosmetics, in particular to a composition containing recombinant human albumin and an acanthopanax senticosus extract. The composition disclosed by the invention comprises the following components in parts by mass: 5.0-15.0 parts of recombinant human albumin; the mass part of the acanthopanax senticosus extract is 1.0-5.0 parts. In the composition disclosed by the invention, the recombinant human albumin and the acanthopanax senticosus extract have a synergistic effect, so that the proliferation of fibroblasts can be effectively promoted, the skin elasticity can be improved, water can be effectively supplemented, the skin is moisturized and glossy, and the conditions of stains and wrinkles can be effectively improved. The composition disclosed by the invention is simple in components and good in biocompatibility, and can be widely applied to preparation of anti-aging products.

Description

Technical Field

[0001] The invention relates to the field of cosmetics, and in particular to a composition containing recombinant human albumin and acanthopanax senticosus extract. Background Art

[0002] Human skin is an organ that easily ages. As we age, the aging characteristics of the skin also appear: more wrinkles, sagging and yellowing of the skin, reduced luster, luster and smoothness, coarsening of the texture, increased pigmentation or the appearance of depigmentation spots, etc. It is generally believed that the causes of skin aging are mainly endogenous factors and exogenous factors. Endogenous factors include: (1) genetic factors, because the order of chromosome polymerization reactions produced by genetic inheritance will change with age; (2) hormone shortage, due to hormone deficiency, hormone aging occurs. For example, estrogen deficiency will reduce the function of the skin's connective tissue and cause various skin changes. Exogenous factors include: (1) pollution, namely, the pollution caused by the growing exhaust gas and various pollutants in the atmosphere forms a large number of free radicals. The attack of free radicals leads to the death of skin cells and rapid aging, such as skin suffocation, blocked pores, and skin fragility; (2) photoaging, namely, the accumulation of UVA / UVB rays produces some very harmful effects on the skin, such as dermal tissue disorder, thinning of the epidermal dermal connective tissue that controls skin fine-tuning, etc. In addition, excessive ultraviolet exposure is also the main cause of premature aging symptoms on facial skin.

[0003] Anti-aging cosmetics target the characteristics and causes of aging skin. On the basis of moisturizing and sun protection, they add active nutrients to enhance skin nutrient absorption, while further smoothing the skin to achieve the purpose of delaying aging and removing wrinkles.

[0004] With the restriction of the use of many synthetic chemicals, "green" has become the development direction of cosmetics in the 21st century. Consumers' demand for safe and non-irritating cosmetics has promoted the application of natural active ingredients in cosmetics. Natural ingredients with physiological activities such as anti-free radical, anti-oxidation, anti-radiation, anti-bacterial, and anti-inflammatory are increasingly valued by people. At present, the development of natural active ingredients that can be used in anti-aging cosmetics has become a trend.

[0005] Human serum albumin (HSA) is a monomeric protein produced by the liver. Mature human serum albumin contains 585 amino acid residues and has a molecular weight of 66KD. Albumin has abundant free sulfhydryl groups and antioxidant properties. It can scavenge free radicals (such as hypochlorous acid (HOCl) and peroxynitrite (ONOOH)) and provide sulfhydryl groups (-SH) to produce anti-aging effects.

[0006] Acanthopanax senticosus (Rupr. et Maxim) Harms, a plant of the Araliaceae family, is a dried root and rhizome or stem. It has been recorded in Shennong's Herbal Classic and listed as a top-grade product. It is mainly distributed in Korea, Japan, Russia and Northeast China. It has a pungent, slightly bitter, warm nature and enters the spleen, kidney and heart meridians. It has the effects of invigorating qi and strengthening the spleen, nourishing the kidney and calming the mind. Acanthopanax senticosus is rich in flavonoids, saponins and other compounds. Modern pharmacological studies have shown that it has significant effects in anti-oxidation, anti-inflammatory, and improving immunity, and has extremely high economic and medicinal value.

[0007] Existing cosmetics use chemical reagents or plant extracts as main ingredients. Chemical reagents have poor biocompatibility and are prone to cause inflammation, redness, swelling, allergies, pain and other problems. Plant extracts are subject to the extraction process, resulting in low and fluctuating active ingredient content, which leads to a high amount of plant extracts and increased costs. Therefore, it is difficult for existing cosmetics to meet both biocompatibility and antioxidant effects at the same time, and it is difficult to meet the skin's antioxidant needs. Summary of the invention

[0008] In view of the deficiencies in the prior art, the first aspect of the present invention provides a recombinant human albumin anti-aging composition, which comprises: recombinant human albumin and Acanthopanax senticosus extract, wherein the mass fraction of the recombinant human albumin is 5.0 to 15.0 parts; the mass fraction of the Acanthopanax senticosus extract is 1.0 to 5.0 parts.

[0009] Furthermore, the composition also includes additives acceptable in the field of cosmetics.

[0010] Furthermore, the composition also includes at least one of a moisturizer, an emollient, a film former, an antioxidant, a whitening agent, a preservative, a fragrance, an emulsifier, a thickener, and a stabilizer.

[0011] Furthermore, the moisturizer includes at least one of glycerin, hydrogenated lecithin, sodium hyaluronate, ceramide, squalane, betaine, allantoin, butylene glycol, and polyethylene glycol; the emollient includes at least one of polydimethylsiloxane, isopropyl palmitate, isopropyl myristate, jojoba oil, olive oil, seaweed extract, and palmitoyl pentapeptide-3; the film-forming agent includes at least one of polydimethylsiloxane, polyvinyl alcohol, hydroxyethyl cellulose, chitosan, and sodium alginate; the antioxidant includes at least one of vitamin E, green tea extract, coenzyme Q10, ferulic acid, astaxanthin, vitamin C derivatives, and resveratrol; the whitening agent includes niacinamide, licorice extract, vitamin C derivatives, tranexamic acid, and bearberry. The invention discloses a kind of hydroxyethylcellulose gum, tragacanth gum, gum arabic, magnesium aluminum silicate, carbomer and acrylic acid. ...

[0012] Furthermore, the dosage form of the composition is selected from at least one of cream, ointment, water, lotion, essence, gel, oil, spray, powder and mask.

[0013] It should be noted that the present invention does not strictly limit the composition and weight percentage of acceptable additives in the field of cosmetics. All one or more additives that are harmless to the human body and have the functions of moisturizers, emollients, film formers, antioxidants, whitening agents, preservatives, fragrances, emulsifiers, thickeners, stabilizers, etc., which are selected according to the dosage form of the product, or the weight percentage of the additives is routinely adjusted according to actual needs, are all protected by the present invention.

[0014] The second aspect of the present invention provides the use of the composition in at least one of the following aspects: A1: Application in the preparation of products for increasing the activity of fibroblasts; A2: Application in the preparation of products that increase skin activity; A3: Application in the preparation of products for increasing skin glossiness; A4: Application in the preparation of products for replenishing skin moisture; A5: Application in the preparation of products for lightening pigmentation spots; A6: Application in the preparation of products for reducing fine lines; A7: Application in the preparation of anti-aging products.

[0015] Further, the product includes cosmetics, and further, the product includes facial cosmetics. Preferably, the facial cosmetics include at least one of face cream, essence, sunscreen, and moisturizing gel.

[0016] In a third aspect, the present invention provides a recombinant human albumin anti-aging facial cream, the anti-aging facial cream comprising: 5.0-15.0 parts by weight of recombinant human albumin; 1.0-5.0 parts by weight of Acanthopanax senticosus extract; 2.0-12.0 parts by weight of polydimethylsiloxane; 0.5-10.0 parts by weight of isopropyl palmitate; 1.5-8.0 parts by weight of hydrogenated lecithin; 2.0-8.0 parts by weight of glycerol; 0.05-0.25 parts by weight of tragacanth gum; 0.05-0.30 parts by weight of gum arabic; 0.01-0.20 parts by weight of polysorbate; 0.05-0.40 parts by weight of carbomer; and 0.01-0.5 parts by weight of chlorphenesin.

[0017] Furthermore, in the anti-aging cream, polydimethylsiloxane is a film-forming agent and an emollient; isopropyl palmitate is an emollient; hydrogenated lecithin is a moisturizer and an emulsifier; glycerin is a moisturizer; tragacanth gum is a thickener and a stabilizer; gum arabic is a thickener and a stabilizer; carbomer is a polymer thickener; polysorbate is an emulsifier; and chlorphenesin is a preservative.

[0018] In a fourth aspect, the present invention provides a method for preparing the composition, the method comprising the following steps: S1: Preparation of Acanthopanax senticosus extract: Grind Acanthopanax senticosus extract, pass through a 10-mesh sieve to obtain powder, soak it in 70%-85% (v / v) ethanol at a material-liquid mass ratio of 1:(3-8), reflux extract at a water bath temperature of 60-100°C, collect the filtrate, distill the filtrate to remove ethanol until there is no alcohol taste, add deionized water to dissolve the Acanthopanax senticosus extract and mix well, spray dry the mixed solution to obtain the Acanthopanax senticosus extract; S2: Dissolve the mass fraction of Acanthopanax senticosus extract into a recombinant human albumin aqueous solution or a recombinant human albumin saline buffer, and use a high-pressure homogenizer at 500-1500 bar for high-pressure homogenization for 25-35 min at a temperature of 25-50° C. to obtain a uniform solution of the composition. Preferably, the recombinant human albumin saline buffer comprises at least one of recombinant human albumin phosphate buffer, recombinant human albumin citrate buffer, and recombinant human albumin acetate buffer. Preferably, the recombinant human albumin buffered saline solution is a recombinant human albumin PBS buffer.

[0019] Furthermore, the spray drying conditions in step S1 are: spray drying air inlet temperature: 120-160° C., atomization pressure: 0.1-1.0 MPa, and feed rate: 400-800 ml / h.

[0020] Further, the ethanol concentration in step S1 is 70%, 73%, 75%, 78%, 80%, 83%, 85% or any value therebetween; the mass ratio of Acanthopanax senticosus to ethanol is 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8 or any value therebetween. Further, the Acanthopanax senticosus extract is soaked in ethanol for 4 to 8 hours.

[0021] Furthermore, the extraction time of the water bath extraction step is 15 to 25 minutes; further, the water bath extraction step can be repeated 2 to 5 times, further, the water bath temperature extraction step can be repeated 2, 3, 4, 5 times, preferably, the step is repeated 3 times; further, the repeatedly extracted Acanthopanax extract filtrates need to be combined and then distilled.

[0022] Furthermore, the method further comprises the step of adding additives acceptable in the cosmetics field to the composition to prepare the cosmetics.

[0023] In a specific embodiment of the present invention, a method for preparing an anti-aging cream containing recombinant human albumin using the composition is provided, the method comprising the following steps: (1) The Acanthopanax senticosus extract is prepared according to the following method: the Acanthopanax senticosus used in the formula is crushed, passed through a 10-mesh sieve to obtain a powder, soaked in 75% ethanol at a solid-liquid ratio of 1:(3-8) for 4-8 hours, refluxed for extraction at a water bath temperature of 60-100°C for 15-25 minutes, extracted 3-5 times, the filtrate is combined, the ethanol is distilled off until there is no alcohol taste, the Acanthopanax senticosus extract is suspended in deionized water, and the suspended solution is spray-dried to obtain the Acanthopanax senticosus extract; (2) Inlet air temperature for spray drying: 120~160℃, atomization pressure: 0.1~1.0Mpa, feed rate: 400~800ml / h; (3) Stir hydrogenated lecithin, gum arabic, gum tragacanth, polydimethylsiloxane, isopropyl palmitate and chlorphenesin according to their weight proportions at 40-80 rpm, 75-105°C and 15-50 min to obtain phase A; stir glycerol, polysorbate and carbomer according to their weight proportions at 35-80 rpm, 55-85°C and 15-45 min to obtain phase B; add phase A and phase B and spray-dried powder of Acanthopanax senticosus extract of weight proportions to recombinant human albumin aqueous solution or buffered saline solution, and homogenize with a high-pressure homogenizer at 500-1500 bar for 30 min at 25-50°C to obtain a mixed solution; (4) The mixed solution after high pressure homogenization is placed in a -4°C refrigerator for 24 hours to obtain the recombinant human albumin anti-aging cream.

[0024] The beneficial effects of the present invention include but are not limited to: 1. In the composition of the present invention, recombinant human albumin and Acanthopanax senticosus extract work synergistically to effectively promote the proliferation of fibroblasts, increase skin elasticity, effectively replenish water, make the skin moisturized and shiny, and effectively improve spots and wrinkles. The composition of the present invention has simple ingredients and good biocompatibility, and can be widely used in the preparation of anti-aging skin care products; 2. The preparation method of the composition of the present invention has a simple production process and has a high application prospect. DETAILED DESCRIPTION

[0025] The present invention is described in detail below with reference to examples, but the present invention is not limited to these examples. Unless otherwise specified, the raw materials and catalysts in the examples of the present invention are purchased through commercial channels.

[0026] Definition: Recombinant human albumin refers to albumin prepared using recombinant DNA technology for treatment and prevention.

[0027] Source of materials: Recombinant human albumin: Tonghua Anrui Special Biopharmaceutical Co., Ltd., batch number: ART101S; Acanthopanax senticosus was purchased from Kangmei Xinkaihe (Jilin) ​​Pharmaceutical Co., Ltd., batch number: 20231120; equipment: Water bath (HH.S21-8, Shanghai Boxun Medical Biological Instrument Co., Ltd.); Multifunctional fluorescence microplate reader (SPMax 3500FL, Shanghai Shanpu Biotechnology Co., Ltd.); Analytical balance (BSA224S, Sartorius Scientific Instruments Beijing Co., Ltd.); Human fibroblasts (Lot No.: CBP61613, Nanjing Kebai Biotechnology Co., Ltd.); pH meter (PB-10, Sartorius Scientific Instruments Beijing Co., Ltd.); Facial image analysis: VISIA image analysis software (Canfied, USA); Skin elasticity test probe Cutometer (MPA580, CK Company, Germany); Skin microscope and active skin surface analysis system VisioScan (VC20plus, CK Company, Germany); Unless otherwise specified, other reagents and instruments used were commercially available.

[0028] Example 1 Preparation of an anti-aging composition containing recombinant human albumin comprising the following components in parts by weight: The effective ingredients are shown in Table 1.

[0029] Table 1 Compositions of various anti-aging compositions containing recombinant human albumin

[0030] The specific steps for preparing an anti-aging composition containing recombinant human albumin are as follows: (1) The Acanthopanax senticosus extract is prepared by the following method: the Acanthopanax senticosus used in the formula is crushed, passed through a 10-mesh sieve to obtain a powder, soaked in 75% ethanol at a solid-liquid ratio of 1:5 for 6 hours, refluxed for 15 minutes at a water bath temperature, extracted three times, the filtrates are combined, and the ethanol is distilled off until there is no alcohol taste, the Acanthopanax senticosus extract is suspended in purified water, the above solutions are mixed evenly, and the mixed solution is spray-dried to obtain the Acanthopanax senticosus extract; (2) Inlet air temperature for spray drying: 120-160°C, preferably 150°C; atomization pressure: 0.1-1.0 MPa, preferably 0.5 MPa; feed rate: 200-800 ml / h, preferably 450 ml / h; (3) Add the spray-dried powder of Acanthopanax senticosus extract to the aqueous solution of recombinant human albumin, and homogenize at a pressure of 500 to 1500 bar for 30 minutes at a temperature of 25 to 50° C. using a high-pressure homogenizer to obtain a mixed solution.

[0031] Test Example 1 Cell Activity Test Based on the T / ZHCA021-2022 standard, this experiment uses the CCK-8 method for corresponding optimization. The effects of an anti-aging composition of the present invention (groups 1 to 11) and a blank control group (group 12) on the viability of human fibroblasts were determined: cells in a good logarithmic growth phase were cultured at 5×10 3The cells were inoculated at a density of 100 μl / 100 μl in a 96-well plate and incubated overnight at 37°C in a 5% CO2 constant temperature incubator. The cells were treated with groups (1-12) and cultured for 24 h. 10 μl of CCK-8 reagent was added to each well and incubated at 37°C for 1 h. The OD was measured. 450nm The cell viability was calculated using the OD 450nm Value, denoted as A s OD of control group and blank group 450nm Value, denoted as A c and A b All samples were measured three times. Cell activity was calculated according to the following formula, and the experimental data are shown in Table 2.

[0032] Cell viability (%) = [(A s -A b ) / (A c -A b )]×100% A s : Absorbance of the experimental group (containing cells, culture medium, CCK-8 solution and anti-aging composition); A c : Absorbance of the control group (containing cells, culture medium, CCK-8 solution, but not containing the anti-aging composition); A b : Absorbance of blank group (containing culture medium and CCK-8 solution, but not containing cells or anti-aging composition).

[0033] Table 2 Cell activity test results

[0034] Note: Compared with the blank control group, P≤0.05, * has significant difference.

[0035] Effect of the anti-aging composition on fibroblast viability: The effects of the prepared groups (1~11) on the viability of fibroblasts were investigated. The results showed that the cell survival rates were all above 102%, indicating that the prepared recombinant human albumin anti-aging composition was non-toxic to cells, indicating that the recombinant human albumin anti-aging composition can effectively promote the proliferation of fibroblasts.

[0036] At the same time, compared with the experimental groups (Groups 1 to 5), the experimental data of the experimental groups without adding recombinant human albumin and Acanthopanax senticosus extract (Groups 6 to 7) and adding animal and plant albumin (Groups 8 to 9) showed lower cell survival rates, indicating that the anti-aging composition can only maintain high cell activity under the premise of the coexistence of recombinant human albumin and Acanthopanax senticosus extract. At the same time, compared with the blank control group, the cell activity value of the experimental group was >120%, and the experimental group 3 had significant differences compared with other groups, with a cell activity of 125.5±0.16%.

[0037] Test Example 2 Human skin irritation test According to the "Technical Specifications for Safety of Cosmetics" (2015 edition), human patch tests were conducted. 20 males and 20 females aged 20 to 30 were selected as subjects (without skin diseases and iatrogenic skin sensitivity). Patch tests were conducted on the inner forearms and behind the ears of the subjects. For the patch test behind the ears, the test sample was removed after 30 minutes of the test. For the patch test on the inner forearm, the test sample was removed after 0.5, 12, and 24 hours of the test. After the test, the skin reaction was observed and evaluated with reference to Table 3.

[0038] Table 3 Evaluation criteria for skin patch test

[0039] The experimental results showed that the patch test on the skin of the subjects showed that the patch test results of all 40 subjects were negative, which showed that the composition prepared by the invention patent had no obvious irritation and sensitization to the human body, that is, it had good safety performance.

[0040] Test Example 3 Skin Anti-aging Test Test population screening requirements According to the product testing requirements, the experimental research subjects are 210 healthy women aged 30-60 years old, including 70 people aged 30-40 years old, 70 people aged 41-50 years old, and 70 people aged 51-60 years old. Subjects were selected according to the standards of the "Technical Specifications for Safety of Cosmetics" (2015 edition). According to the experimental requirements, those with obvious facial wrinkles, spots and other aging phenomena were selected. The grouping information is shown in Table 4.

[0041] Table 4 Skin anti-aging test group information

[0042] After cleansing the face, volunteers took an appropriate amount of the anti-aging composition prepared by groups 1, 3, 5, 6, 7, 10, and 11 in Example 1 and applied it to the face. Massage gently until absorbed. Apply once a day in the morning and evening for 30 consecutive days. Test once before use and once after 1 month. The test results are averaged. The elasticity, skin gloss, moisture content, number of spots (pieces / cm 2 )、Texture depth (μm), Wrinkle density (lines / cm 2 ) was used as the evaluation index, and the anti-aging compositions prepared from groups 1, 3, 5, 6, 7, 10, and 11 in Example 1 were used for testing. The data results are shown in Tables 5 to 8.

[0043] Table 5 Test results of elasticity, brightness and moisture content before use

[0044] Table 6 Number of spots before use (pcs / cm 2 )、Texture depth (μm), Wrinkle density (lines / cm 2 ) Test Results

[0045] Table 7 Test results of elasticity, brightness and moisture content after use

[0046] Table 8 Number of spots after use (pcs / cm 2 )、Texture depth (μm), Wrinkle density (lines / cm 2 ) Test Results

[0047] From the data in Tables 5 to 8, it can be seen that the average scores of various scores have increased to a certain extent when using the anti-aging composition prepared by the patent of the present invention, indicating that the use of the composition can improve skin elasticity, glossiness and increase skin moisture content in a short period of time, and effectively improve the condition of spots and wrinkles.

[0048] Example 2 Preparation of an anti-aging facial cream containing recombinant human albumin comprising the following components in parts by mass: The effective ingredients are shown in Table 9.

[0049] Table 9 Ingredients of various anti-aging creams containing recombinant human albumin

[0050] The specific steps of preparing an anti-aging facial cream containing recombinant human albumin are as follows: (1) The Acanthopanax senticosus extract is prepared by the following method: the Acanthopanax senticosus used in the formula is crushed, passed through a 10-mesh sieve to obtain a powder, soaked in 75% ethanol at a solid-liquid ratio of 1:5 for 6 hours, refluxed for 15 minutes at a water bath temperature, extracted three times, the filtrates are combined, and the ethanol is distilled off until there is no alcohol taste, the Acanthopanax senticosus extract is suspended in purified water, the above solutions are mixed evenly, and the mixed solution is spray-dried to obtain the Acanthopanax senticosus extract; (2) Inlet air temperature for spray drying: 120-160°C, preferably 150°C; atomization pressure: 0.1-1.0 MPa, preferably 0.5 MPa; feed rate: 200-800 ml / h, preferably 450 ml / h; (3) Stir hydrogenated lecithin, gum arabic, gum tragacanth, polydimethylsiloxane, isopropyl palmitate and chlorphenesin according to their weight proportions at 40-80 rpm, 75-105°C and 15-50 min to obtain phase A; stir glycerol, polysorbate and carbomer according to their weight proportions at 35-80 rpm, 55-85°C and 15-45 min to obtain phase B; add phase A and phase B and spray-dried powder of Acanthopanax senticosus extract of weight proportions to recombinant human albumin, and homogenize with a high-pressure homogenizer at 500-1500 bar for 30 min at 25-50°C to obtain a mixed solution; (4) The mixed solution after high pressure homogenization is placed in a -4°C refrigerator for 24 hours to obtain the recombinant human albumin anti-aging cream.

[0051] Test Example 4 Cell Activity Test The anti-aging cream prepared in Example 2 was used to replace the anti-aging composition in Test Example 1, and the experimental steps of Test Example 1 were repeated. The experimental results are shown in Table 10.

[0052] Table 10 Cell activity test results

[0053] Note: Compared with the blank control group, P≤0.05, * has significant difference.

[0054] Effect of anti-aging cream on fibroblast viability: The cell viability of all experimental groups containing active ingredients (groups 1-5, 8-9) was >103% (vs. blank control group), among which the recombinant human albumin group (group 3) performed best, with a cell viability of 135.7±0.13% (p<0.05), confirming that the cream formula is not only non-cytotoxic, but also has a significant pro-proliferation effect on fibroblasts. The cell viability of the single-ingredient missing experimental group (groups 6-7, without the simultaneous addition of recombinant human albumin and Acanthopanax senticosus extract) was lower than that of the full-ingredient group (groups 1-5), indicating that the two core active ingredients have a synergistic mechanism, and their combined use is a necessary condition for achieving high cell activity. The ratio experiment showed (groups 1-5) that with the increase of the concentration of recombinant human albumin, the cell viability showed a trend of first rising and then stabilizing, and the optimal effect concentration appeared in group 3, indicating that the cell proliferation effect was the strongest under the conditions of a specific ratio.

[0055] Test Example 5 Hygiene Index Determination Experiment and Heavy Metal Determination According to the microbial and heavy metal test methods of the "Safety Technical Specifications for Cosmetics" (2015 edition), the total colony count, heat-resistant Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, molds, yeasts and heavy metals in a recombinant human albumin anti-aging cream (Groups 1-5) were determined. The microbiological index data are shown in Table 11, and the heavy metal detection test results are shown in Table 12.

[0056] Table 11 Results of microbiological index determination in each group

[0057] It can be seen from Table 11 that the test results of various indicators in the hygiene of anti-aging creams (groups: 1~5) are in compliance with the regulations.

[0058] Table 12 Results of heavy metal index determination in each group

[0059] It can be seen from Table 12 that the heavy metal detection index test results of anti-aging creams (groups: 1~5) are in compliance with the regulations.

[0060] Test Example 6 Human skin irritation test The anti-aging cream prepared in Example 2 was used to replace the anti-aging composition in Test Example 2 and the experimental steps of Test Example 2 were repeated.

[0061] The experimental results showed that the patch test on the skin of the subjects showed that the patch test results of all 40 subjects were negative, which showed that the facial cream prepared by the invention patent had no obvious irritation and allergenicity to the human body, that is, it had good safety performance.

[0062] Test Example 7 Skin Anti-Aging Test The experimental steps of Test Example 4 were repeated by replacing the anti-aging composition in Test Example 3 with the anti-aging creams of Groups 1, 3, and 5 prepared in Example 2, and the grouping information is shown in Table 13. The experimental results are shown in Tables 14 to 17.

[0063] Table 13 Skin anti-aging test group information

[0064] Table 14 Elasticity, brightness and moisture content test results before use

[0065] Table 15 Number of spots before use (pcs / cm 2 )、Texture depth (μm), Wrinkle density (lines / cm 2 ) Test Results

[0066] Table 16 Test results of elasticity, brightness and moisture content after use

[0067] Table 17 Number of spots after use (pcs / cm 2 )、Texture depth (μm), Wrinkle density (lines / cm 2 ) Test Results

[0068] From the data in Tables 14 to 17, it can be seen that the average scores of various scores of the anti-aging cream prepared by the patent of the present invention have increased to a certain extent, indicating that the use of the cream can improve skin elasticity, glossiness and increase skin moisture content in a short period of time, and effectively improve spots and wrinkles.

[0069] The above description is only an embodiment of the present invention. The protection scope of the present invention is not limited by these specific embodiments, but is determined by the claims of the present invention. For those skilled in the art, the present invention may have various changes and variations. Any modification, equivalent substitution, improvement, etc. made within the technical ideas and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A recombinant human albumin anti-aging composition, characterized in that: The composition comprises: recombinant human albumin and Acanthopanax senticosus extract, wherein the mass fraction of the recombinant human albumin is 5.0 to 15.0 parts; the mass fraction of the Acanthopanax senticosus extract is 1.0 to 5.0 parts.

2. The composition according to claim 1, characterized in that The composition may further include additives acceptable in the cosmetic field.

3. The composition according to claim 1, characterized in that The composition further comprises at least one of a moisturizer, an emollient, a film former, an antioxidant, a whitening agent, a preservative, a fragrance, an emulsifier, a thickener, and a stabilizer.

4. The composition according to claim 1, characterized in that The dosage form of the composition is selected from at least one of cream, ointment, water, milk, essence, gel, oil, spray, powder and mask.

5. The use of the composition according to any one of claims 1 to 4 in at least one of the following aspects: A1: Application in the preparation of products for increasing the activity of fibroblasts; A2: Application in the preparation of products that increase skin activity; A3: Application in the preparation of products for increasing skin glossiness; A4: Application in the preparation of products for replenishing skin moisture; A5: Application in the preparation of products for lightening pigmentation spots; A6: Application in the preparation of products for reducing fine lines; A7: Application in the preparation of anti-aging products.

6. The use according to claim 5, characterized in that: The products include cosmetics.

7. A recombinant human albumin anti-aging facial cream, characterized in that: The anti-aging facial cream comprises: the composition according to claim 1; 2.0 to 12.0 parts by weight of polydimethylsiloxane; 0.5 to 10.0 parts by weight of isopropyl palmitate; 1.5 to 8.0 parts by weight of hydrogenated lecithin; 2.0 to 8.0 parts by weight of glycerol; 0.05 to 0.25 parts by weight of tragacanth; 0.05 to 0.30 parts by weight of gum arabic; 0.01 to 0.20 parts by weight of polysorbate; 0.05 to 0.40 parts by weight of carbomer; and 0.01 to 0.5 parts by weight of chlorphenesin.

8. A method for preparing the composition according to any one of claims 1 to 4, characterized in that: The method comprises the following steps: S1: Preparation of Acanthopanax senticosus extract: Grind Acanthopanax senticosus extract, pass through a 10-mesh sieve to obtain powder, soak it in 70%-85% (v / v) ethanol at a material-liquid mass ratio of 1:(3-8), reflux extract at a water bath temperature of 60-100°C, collect the filtrate, distill the filtrate to remove ethanol until there is no alcohol taste, add deionized water to dissolve the Acanthopanax senticosus extract and mix well, spray dry the mixed solution to obtain the Acanthopanax senticosus extract; S2: Dissolve the Acanthopanax senticosus extract in the recombinant human albumin aqueous solution or salt buffer, and use a high-pressure homogenizer at 500-1500 bar for 25-35 minutes at a temperature of 25-50° C. to obtain a uniform solution of the composition.

9. The method according to claim 8, characterized in that The spray drying conditions in step S1 are: spray drying air inlet temperature: 120-160° C., atomization pressure: 0.1-1.0 MPa, and feed rate: 400-800 ml / h.

10. The method according to claim 8, characterized in that The method further comprises the step of adding additives acceptable in the cosmetics field to the composition to prepare the cosmetic.

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