Papain composition as well as preparation method and application thereof

Abstract

The invention provides a papain composition as well as a preparation method and application thereof, and relates to the technical field of cleaning cosmetics. The papain composition provided by the invention comprises lysozyme, superoxide dismutase, papain, lactoperoxidase, glucose oxidase, oxidoreductase and bromelain. The papain composition, the cassia obtusifolia seed extract, the guava fruit extract, the sugarcane extract and the kelp extract are used for cleaning cosmetics, the skin can be deeply cleaned, and grease and dirt can be effectively removed; cutin is mildly removed, skin cell renewal is promoted, and the skin is smooth as silky; acnes are remarkably removed, skin inflammation is relieved, and skin discomfort is relieved; the skin color is brightened, the darkness is improved, and the skin has natural luster; the skin metabolism can be promoted, the skin tendering effect is achieved, redundant fat is decomposed, the fat dissolving effect is achieved, and all-around care and improvement are brought to the skin.

Description

Technical Field

[0001] The invention relates to the technical field of cleansing cosmetics, and in particular to a papain composition and a preparation method and application thereof. Background Art

[0002] Traditional cleansing cosmetics mainly rely on surfactants to achieve the cleansing function. However, many commonly used surfactants, such as sodium lauryl sulfate, have strong cleaning ability, but often have some disadvantages. On the one hand, they may over-clean the skin, destroy the skin's natural barrier function, and cause problems such as dry, tight, and sensitive skin. Long-term use may aggravate the loss of moisture in the skin and affect the skin's moisturizing ability and elasticity. On the other hand, some surfactants are irritating to the skin and may cause skin discomfort, especially for people with sensitive skin, who are prone to symptoms such as redness, swelling, and itching after use.

[0003] In recent years, the application of enzymes as a natural biocatalyst in cleaning cosmetics has gradually attracted attention. Enzymes are highly specific and efficient, and can catalyze specific chemical reactions under relatively mild conditions, thereby achieving multiple functions such as cleaning and care. Compared with traditional chemical cleaners, enzymes are less irritating to the skin during the cleaning process and have better biocompatibility. For example, proteases can decompose protein dirt on the skin surface, and lipases can decompose oily dirt. They can act on different types of dirt, and their action mode is relatively mild, and it is not easy to cause excessive damage to the skin.

[0004] Papain is a plant protease extracted from papaya, which has unique properties and advantages. It can effectively decompose a variety of proteins, including keratin on the surface of the skin, protein components in dirt, and some protein substances related to skin problems, such as proteins secreted by certain bacteria that cause acne. Papain is active in a wide range of temperature and pH, which enables it to adapt to different cosmetic formulation environments. However, the use of papain alone in cleansing cosmetics also faces some challenges, such as stability, compatibility with other ingredients, enzymolysis efficiency, etc., which need to be solved through reasonable composition design. Therefore, it is of great practical significance to develop a cleansing cosmetic based on a papain composition, which can not only utilize the cleaning and skin care advantages of papain, but also overcome its limitations when used alone, meet consumers' needs for efficient, mild, and multifunctional cleansing cosmetics, and have broad innovation and development space in the field of cleansing cosmetic technology. Summary of the invention

[0005] In view of the above technical problems, the object of the present invention is to provide a papain composition and a preparation method and application thereof. The papain composition provided by the present invention can be used in cleaning cosmetics and has multiple effects such as skin rejuvenation, antibacterial, acne removal, soothing, exfoliation, skin smoothing, brightening, cleaning direction, reshaping skin barrier, and fat dissolving.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions: The invention provides a papain composition, which comprises the following components in parts by weight: 15-25 parts of lysozyme, 20-30 parts of superoxide dismutase, 25-35 parts of papain, 10-20 parts of lactoperoxidase, 3-7 parts of glucose oxidase, 5-15 parts of oxidoreductase and 20-30 parts of bromelain.

[0007] The present invention also provides a method for preparing the papain composition, comprising: mixing lysozyme, superoxide dismutase, papain, lactoperoxidase, glucose oxidase, oxidoreductase and bromelain according to mass fractions to obtain the papain composition.

[0008] The present invention also provides application of the papain composition in preparing facial cleansing cosmetics.

[0009] Preferably, the facial cleansing cosmetics include cleansing milk, cleansing cream, cleansing gel, cleansing foam and cleansing mask.

[0010] The present invention also provides a facial cleanser containing the papain composition, comprising the following components in parts by weight: 6-12 parts of the papain composition, 0.5-1.5 parts of Cassia obtusifolia seed extract, 3-6 parts of guava fruit extract, 7-13 parts of sugarcane extract, 3-6 parts of kelp extract, 5-15 parts of camellia seed oil, 5-10 parts of squalane, 10-20 parts of surfactant, 10-20 parts of moisturizer, 1.5-4 parts of thickener, 0.1-0.5 parts of preservative and 40-60 parts of deionized water.

[0011] Preferably, the surfactant is one or more of cocamidopropyl betaine, sodium laureth sulfate, and sodium methyl cocoyl taurate.

[0012] Preferably, the moisturizing agent is one or more of allantoin, glycerol, and pyrrolidone carboxylic acid.

[0013] Preferably, the thickener is one or more of hydroxyethyl cellulose, sodium acetate, PEG-150 distearate, and carbomer.

[0014] Preferably, the preservative is one or more of glyceryl caprylate, methylparaben, benzyl alcohol, and phenoxyethanol.

[0015] The present invention also provides a method for preparing the above-mentioned facial cleanser, comprising: mixing and stirring a thickener, a moisturizer, a Cassia obtusifolia seed extract, a guava fruit extract, a sugarcane extract and a kelp extract to obtain an aqueous phase; mixing and stirring camellia seed oil and squalane to obtain an oil phase; mixing the oil phase and the aqueous phase, adding a surfactant, a preservative and a papain composition, and adjusting the pH value to 6.5-7.5 to obtain a facial cleanser.

[0016] Compared with the prior art, the present invention has the following beneficial effects: The present invention provides a papain composition, comprising: lysozyme, superoxide dismutase, papain, lactoperoxidase, glucose oxidase, oxidoreductase and bromelain. The papain composition provided by the present invention can be used in cleaning cosmetics, can deeply clean the skin, effectively remove grease and dirt; gently remove keratin, promote skin cell renewal, and make the skin smooth as silk; inhibit the growth of harmful bacteria, and exert antibacterial effect; significantly remove acne and soothe skin inflammation, and relieve skin discomfort; brighten skin color, improve dullness, and make the skin glow with natural luster; deeply nourish the skin, reshape the skin barrier function, and strengthen the skin's self-protection ability; it can also promote skin metabolism, achieve skin rejuvenation effect, and decompose excess fat to achieve fat dissolving effect, bringing all-round care and improvement to the skin. DETAILED DESCRIPTION

[0017] The invention provides a papain composition, which comprises the following components in parts by weight: 15-25 parts of lysozyme, 20-30 parts of superoxide dismutase, 25-35 parts of papain, 10-20 parts of lactoperoxidase, 3-7 parts of glucose oxidase, 5-15 parts of oxidoreductase and 20-30 parts of bromelain.

[0018] The lysozyme of the present invention can enhance the self-defense ability of the skin, and can absorb and retain the moisture on the surface of the skin to prevent excessive loss of moisture from the skin. It has a synergistic effect when used in combination with the moisturizing ingredients (capsule, glycerin, pyrrolidone carboxylic acid) in the cleanser, thereby enhancing the moisturizing effect of the entire cosmetics. Superoxide dismutase has an antioxidant effect, prevents free radicals from damaging the skin barrier, and maintains the integrity of the skin barrier. Papain exfoliates, can decompose protein dirt on the skin surface, and is more easily washed away by water, thereby achieving an effective skin cleaning effect. It has a soothing effect on the skin when used in combination with the plant ingredients in the cosmetics. Lactoperoxidase can inhibit a variety of It can inhibit the growth of bacteria, fungi and viruses and reduce skin inflammatory response; glucose oxidase has antibacterial effect, can effectively remove bacteria in acne area, promote the recovery of acne, and can regulate the microecological environment of the skin and enhance the barrier function of the skin. For example, in facial cleanser, it can ensure that beneficial microorganisms on the surface of the skin, such as Staphylococcus epidermidis, can continue to play their protective role on the skin while cleaning the skin; oxidoreductase has antioxidant effect, can prevent the oxidation of easily oxidized ingredients in cosmetics and stabilize cosmetic ingredients; bromelain can gently remove dead skin from the surface of the skin, making the skin smoother and more delicate, improving the skin color, and also has anti-inflammatory and skin-soothing effects.

[0019] The various components of the papain composition of the present invention synergize to enhance the effectiveness, and after being used in cleaning cosmetics, the papain composition can achieve the effects of skin rejuvenation, antibacterial, acne removal, soothing, exfoliation, skin smoothing, brightening, skin barrier remodeling, fat dissolving and the like.

[0020] The present invention also provides a method for preparing the papain composition, comprising: mixing lysozyme, superoxide dismutase, papain, lactoperoxidase, glucose oxidase, oxidoreductase and bromelain according to mass fractions to obtain the papain composition.

[0021] The present invention also provides application of the papain composition in preparing facial cleansing cosmetics.

[0022] The facial cleansing cosmetics of the present invention include facial cleansing milk, facial cleansing cream, facial cleansing gel, facial cleansing foam and facial cleansing mask.

[0023] The present invention also provides a facial cleanser containing the papain composition, comprising the following components in parts by weight: 6-12 parts of the papain composition, 0.5-1.5 parts of Cassia obtusifolia seed extract, 3-6 parts of guava fruit extract, 7-13 parts of sugarcane extract, 3-6 parts of kelp extract, 5-15 parts of camellia seed oil, 5-10 parts of squalane, 10-20 parts of surfactant, 10-20 parts of moisturizer, 1.5-4 parts of thickener, 0.1-0.5 parts of preservative and 40-60 parts of deionized water.

[0024] The Cassia obtuse seed extract of the present invention has anti-inflammatory, anti-oxidation and anti-redness effects; the guava fruit extract has good anti-lipid peroxidation effect, delays skin aging, and has anti-inflammatory and astringent effects, and can also inhibit the activity of B-16 melanocytes and has a whitening effect; the sugarcane extract can absorb and retain moisture on the skin surface and has a moisturizing effect; removes aged keratin on the skin surface, makes the skin smoother and more delicate, promotes skin metabolism, is beneficial to the absorption of subsequent skin care products, reduces skin inflammation, and reshapes the skin barrier; the kelp extract can absorb dirt, grease and impurities on the skin surface and has a cleaning effect It is good and can form a moisturizing layer on the skin surface to improve the moisturizing effect, and it has antioxidant and skin soothing effects; camellia seed oil can dissolve grease and dirt on the skin surface, such as sebum, oil components in makeup residues, etc., which are more easily washed away by water, thereby achieving the purpose of cleansing the skin, and can reduce inflammation and relieve skin discomfort. The antioxidant ingredients it contains can delay the aging process of the skin and keep the skin healthy and young; squalane can blend well with the skin, is easily absorbed by the skin, and prevents the moisture inside the skin from evaporating through the epidermis. It has a synergistic effect with moisturizers to enhance the moisturizing effect of the skin.

[0025] The surfactant of the present invention is preferably one or more of cocamidopropyl betaine, sodium laureth sulfate, and sodium methyl cocoyl taurate; the moisturizer is preferably one or more of allantoin, glycerin, and pyrrolidone carboxylic acid; the thickener is preferably one or more of hydroxyethyl cellulose, sodium acetate, PEG-150 distearate, and carbomer; the preservative is preferably one or more of glyceryl caprylate, methylparaben, benzyl alcohol, and phenoxyethanol.

[0026] The various components of the facial cleanser of the present invention have a synergistic effect and have the effects of skin rejuvenation, antibacterial, acne removal, soothing, exfoliation, skin smoothing, brightening, cleaning direction, reshaping the skin barrier, dissolving fat and the like.

[0027] The present invention also provides a method for preparing the above-mentioned facial cleanser, comprising: mixing and stirring a thickener, a moisturizer, a Cassia obtusifolia seed extract, a guava fruit extract, a sugarcane extract and a kelp extract to obtain an aqueous phase; mixing and stirring camellia seed oil and squalane to obtain an oil phase; mixing the oil phase and the aqueous phase, adding a surfactant, a preservative and a papain composition, and adjusting the pH value to 6.5-7.5 to obtain a facial cleanser.

[0028] The method of using the facial cleanser of the present invention is as follows: after washing both hands, wet the face with warm water, put the facial cleanser in the palm of the hand, add a small amount of water, gently rub both hands and evenly apply it on the face, gently massage in a circular manner with the fingertips, thoroughly rinse the facial cleanser foam on the face with warm water, and gently dry the face with a clean, soft towel.

[0029] In the present invention, unless otherwise specified, all raw material components are commercially available products well known to those skilled in the art.

[0030] The technical solutions in the present invention will be described clearly and completely below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] In the following embodiments, unless otherwise specified, all of them are conventional methods.

[0032] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0033] In the following examples and comparative examples, lysozyme, superoxide dismutase, papain, lactoperoxidase, glucose oxidase, oxidoreductase and bromelain were purchased from Guangzhou Boshipai Biotechnology Co., Ltd., and the enzyme activities of lysozyme, superoxide dismutase, papain, lactoperoxidase, glucose oxidase, oxidoreductase and bromelain were all 5 billion IU.

[0034] Example 1 Papain Composition: 20 parts by weight of lysozyme, 25 parts by weight of superoxide dismutase, 30 parts by weight of papain, 15 parts by weight of lactoperoxidase, 5 parts by weight of glucose oxidase, 10 parts by weight of oxidoreductase and 25 parts by weight of bromelain were mixed to obtain a papain composition.

[0035] Example 2 Papain Composition: 15 parts by weight of lysozyme, 20 parts by weight of superoxide dismutase, 25 parts by weight of papain, 10 parts by weight of lactoperoxidase, 3 parts by weight of glucose oxidase, 5 parts by weight of oxidoreductase and 20 parts by weight of bromelain were mixed to obtain a papain composition.

[0036] Example 3 Papain Composition: 25 parts by weight of lysozyme, 30 parts by weight of superoxide dismutase, 35 parts by weight of papain, 20 parts by weight of lactoperoxidase, 7 parts by weight of glucose oxidase, 15 parts by weight of oxidoreductase and 30 parts by weight of bromelain were mixed to obtain a papain composition.

[0037] Example 4 Papain Composition Cleanser: (1) 45 parts by weight of deionized water is placed in a container, and an agitator is turned on with the speed controlled at 250 r / min. 3 parts by weight of a thickener is slowly added into the deionized water until it is evenly dispersed to form a colloidal solution. 10 parts by weight of the papain composition of Example 1, 15 parts by weight of a moisturizer, 1 part by weight of a Cassia obtusifolia seed extract, 5 parts by weight of a guava fruit extract, 10 parts by weight of a sugar cane extract, and 5 parts by weight of a kelp extract are sequentially added until they are completely dissolved to obtain an aqueous phase. (2) stirring 10 parts by weight of camellia seed oil and 8 parts by weight of squalane at 55° C. to obtain an oil phase; (3) Slowly adding the oil phase to the water phase at a speed of 400 r / min to form a uniform emulsion, adding 15 parts by weight of a surfactant and 0.4 parts by weight of a preservative to the emulsion, adjusting the pH value to 6.0, stopping stirring, filtering with a 100-mesh filter to obtain a uniform and delicate facial cleanser, sterilizing with a 30W ultraviolet lamp for 50 minutes, filling, and packaging; The thickener is composed of hydroxyethyl cellulose and sodium acetate in equal weights, the moisturizer is composed of allantoin and glycerin in a weight ratio of 1:15, the surfactant is cocamidopropyl betaine, and the preservative is composed of glyceryl caprylate and methylparaben in equal weights; The preparation method of the Cassia obtuse seed extract is as follows: crushing the Cassia obtuse seed, passing through a 100-mesh sieve to obtain the Cassia obtuse seed powder, mixing it with a 55% by volume ethanol solution at a solid-liquid ratio of 1:10 g / mL, ultrasonically extracting it at 50° C. and 500 W for 45 minutes, filtering it, and concentrating the filtrate under reduced pressure at 45° C. and 5 kPa to a relative density of 1.2 to obtain the Cassia obtuse seed extract; The preparation method of guava fruit extract is as follows: fresh guava fruit is crushed by a wall breaking machine, mixed with deionized water at a solid-liquid ratio of 1:8 g / mL, firstly adding 0.3% pectinase (enzyme activity of 40,000 U / g) of the mass of guava fruit, enzymolysis for 2 hours at 50°C and pH 4, then adding 0.3% snail protease (enzyme activity of 1.5 U / g) of the mass of guava fruit, enzymolysis for 1.5 hours at 35°C and pH 4, heating to 85°C to inactivate the enzyme for 15 minutes, cooling to room temperature, filtering, spray drying the filtrate at 75°C to a water content of 5%, and obtaining the guava fruit extract; The preparation method of sugarcane extract is as follows: fresh sugarcane is squeezed to obtain sugarcane juice and bagasse, the bagasse is crushed and passed through a 100-mesh sieve, mixed with water at a solid-liquid ratio of 1:10 g / mL, sugarcane juice is added, and a composite microorganism (containing 600 million CFU / g of Bifidobacterium adolescentis) is inoculated at a weight of 3‰ of the bagasse. Bifidobacterium adolescentis CICC ® 6177 and 2 billion Lactobacillus delbrueckii subsp. bulgaricus Lactobacillus delbrueckii subsp. Bulgarian CICC® 6097 according to the mass ratio of 1:2.5) was anaerobically fermented at 40°C and pH 6 for 3 days, the fermentation liquid was filtered, and the filtrate was concentrated under reduced pressure at 60°C and 6 kPa to a relative density of 1.2 to obtain a sugarcane extract; The preparation method of kelp extract is as follows: the dried kelp is crushed, passed through an 80-mesh sieve, mixed with deionized water at a solid-liquid ratio of 1:17 g / mL, firstly 0.6% of the mass of the dried kelp alginate lyase (enzyme activity of 150,000 U / g) is added, enzymolysis is carried out at 33°C and pH 6 for 2 hours, then 3% of the mass of the dried kelp papain (enzyme activity of 60,000 U / g) is added, enzymolysis is carried out at 55°C and pH 6 for 80 minutes, the temperature is raised to 85°C to inactivate the enzyme for 15 minutes, after cooling to room temperature, the filtrate is filtered, and the filtrate is spray-dried at 100°C to a water content of 5%, thereby obtaining the kelp extract.

[0038] Example 5 Papain Composition Cleanser 40 parts by weight of deionized water are placed in a container, and an agitator is turned on with the speed controlled at 200 r / min. 1.5 parts by weight of a thickener is slowly added into the deionized water until it is evenly dispersed to form a colloidal solution. 6 parts by weight of the papain composition of Example 2, 10 parts by weight of a moisturizer, 0.5 parts by weight of an extract of Cassia obtusifolia seeds, 3 parts by weight of a guava fruit extract, 7 parts by weight of a sugarcane extract, and 3 parts by weight of a kelp extract are sequentially added into the colloidal solution until they are completely dissolved to obtain an aqueous phase. (2) stirring 5 parts by weight of camellia seed oil and 5 parts by weight of squalane at 50° C. to obtain an oil phase; (3) Slowly adding the oil phase to the water phase at a speed of 300 r / min to form a uniform emulsion, adding 10 parts by weight of a surfactant and 0.1 parts by weight of a preservative to the emulsion, adjusting the pH value to 5.5, stopping stirring, filtering with an 80-mesh filter to obtain a uniform and delicate facial cleanser, sterilizing with a 30W ultraviolet lamp for 50 minutes, filling and packaging; Among them, the thickener is PEG-150 distearate, the moisturizer is pyrrolidone carboxylic acid and glycerin in a mass ratio of 1:8, the surfactant is sodium laureth sulfate, and the preservative is phenoxyethanol; The preparation method of the Cassia obtuse seed extract is as follows: crushing the Cassia obtuse seed, passing through a 60-mesh sieve to obtain the Cassia obtuse seed powder, mixing it with a 50% by volume ethanol solution at a solid-liquid ratio of 1:8 g / mL, ultrasonically extracting it for 50 minutes at 40° C. and 450 W, filtering it, and concentrating the filtrate under reduced pressure at 40° C. and 4.5 kPa to a relative density of 1.1 to obtain the Cassia obtuse seed extract; The preparation method of guava fruit extract is as follows: fresh guava fruit is crushed, mixed with deionized water at a solid-liquid ratio of 1:6 g / mL, firstly 0.2% of pectinase (enzyme activity of 30,000 U / g) by weight of guava fruit is added, enzymolysis is carried out at 45°C and pH 3.5 for 3 hours, then 0.1% of snail protease (enzyme activity of 5,000 U / g) by weight of guava fruit is added, enzymolysis is carried out at 30°C and pH 3.5 for 2 hours, the temperature is raised to 85°C to inactivate the enzyme for 15 minutes, after cooling to room temperature, filtering, spray drying the filtrate at 70°C to a water content of 8%, and obtaining the guava fruit extract; The preparation method of sugarcane extract is as follows: fresh sugarcane is squeezed to obtain sugarcane juice and bagasse, the bagasse is crushed and passed through an 80-mesh sieve, mixed with water at a solid-liquid ratio of 1:8 g / mL, sugarcane juice is added, and composite microorganisms (containing 500 million CFU / g of Bifidobacterium adolescentis) are inoculated at 2‰ of the weight of the bagasse. Bifidobacterium adolescentis CICC ® 6177 and bacterial activity is 1 billion CFU / g Lactobacillus delbrueckii subsp. bulgaricus Lactobacillus delbrueckii subsp. Bulgarian CICC ® 6097 according to the mass ratio of 1:2) was anaerobically fermented at 35°C and pH 5.5 for 4 days, filtered, and the filtrate was concentrated under reduced pressure at 50°C and 5.3 kPa to a relative density of 1.1 to obtain a sugarcane extract; The preparation method of kelp extract is as follows: the dried kelp is crushed, passed through a 120-mesh sieve, mixed with deionized water at a solid-liquid ratio of 1:15 g / mL, firstly 0.5% of the mass of the dried kelp alginate lyase (enzyme activity of 100,000 U / g) is added, and enzymolysis is carried out at 30°C and pH 5.5 for 3 hours, and then 2% of the mass of the dried kelp papain (enzyme activity of 50,000 U / g) is added, and enzymolysis is carried out at 50°C and pH 5 for 90 minutes, the temperature is increased to 85°C to inactivate the enzyme for 15 minutes, and after cooling to room temperature, the filtrate is filtered, and the filtrate is spray-dried at 90°C to a water content of 7%, thereby obtaining the kelp extract.

[0039] Example 6 Papain Composition Cleanser (1) 60 parts by weight of deionized water is placed in a container, and an agitator is turned on with the speed controlled at 300 r / min. 4 parts by weight of a thickener is slowly added into the deionized water until it is evenly dispersed to form a colloidal solution. 12 parts by weight of the papain composition of Example 3, 15 parts by weight of a moisturizer, 1 part by weight of a Cassia obtusifolia seed extract, 5 parts by weight of a guava fruit extract, 10 parts by weight of a sugar cane extract, and 5 parts by weight of a kelp extract are sequentially added until they are completely dissolved to obtain an aqueous phase. (2) stirring 10 parts by weight of camellia seed oil and 8 parts by weight of squalane at 55° C. to obtain an oil phase; (3) Slowly adding the oil phase to the water phase at a speed of 400 r / min to form a uniform emulsion, adding 15 parts by weight of a surfactant and 0.4 parts by weight of a preservative to the emulsion, adjusting the pH value to 6.0, stopping stirring, filtering with a 100-mesh filter to obtain a uniform and delicate facial cleanser, sterilizing with a 30W ultraviolet lamp for 50 minutes, filling, and packaging; Among them, the thickener is carbomer, the moisturizer is glycerin, the surfactant is sodium methyl cocoyl taurate, and the preservative is benzyl alcohol; The preparation method of the Cassia obtuse seed extract is as follows: crushing the Cassia obtuse seed, passing through a 120-mesh sieve to obtain the Cassia obtuse seed powder, mixing it with a 60% by volume ethanol solution at a solid-liquid ratio of 1:12 g / mL, ultrasonically extracting it at 60° C. and 550 W for 40 minutes, filtering it, and concentrating the filtrate under reduced pressure at 50° C. and 5.5 kPa to a relative density of 1.3 to obtain the Cassia obtuse seed extract; The preparation method of guava fruit extract is as follows: fresh guava fruit is crushed, mixed with deionized water at a solid-liquid ratio of 1:10 g / mL, firstly 0.5% of pectinase (enzyme activity of 50,000 U / g) by weight of guava fruit is added, enzymolysis is carried out at 55°C and pH 4.5 for 1 hour, then 0.4% of snail protease (enzyme activity of 20,000 U / g) by weight of guava fruit is added, enzymolysis is carried out at 40°C and pH 4.5 for 1 hour, the temperature is raised to 85°C to inactivate the enzyme for 15 minutes, after cooling to room temperature, filtering, spray drying the filtrate at 80°C to a water content of 3%, and obtaining the guava fruit extract; The preparation method of sugarcane extract is as follows: fresh sugarcane is squeezed to obtain sugarcane juice and sugarcane bagasse, the sugarcane bagasse is crushed and passed through a 120-mesh sieve, mixed with water at a solid-liquid ratio of 1:12 g / mL, sugarcane juice is added, and a composite microorganism (containing 800 million CFU / g of Bifidobacterium adolescentis) of 5‰ weight of the sugarcane bagasse is inoculated. Bifidobacterium adolescentis CICC ® 6177 and bacterial activity is 3 billion CFU / g Lactobacillus delbrueckii subsp. bulgaricus Lactobacillus delbrueckii subsp. Bulgarian CICC ® 6097 according to the mass ratio of 1:3) was anaerobically fermented at 42°C and pH 6.5 for 4 days, filtered, and the filtrate was concentrated under reduced pressure at 65°C and 6.5 kPa to a relative density of 1.3 to obtain a sugarcane extract; The preparation method of kelp extract is as follows: the dried kelp is crushed, passed through a 60-mesh sieve, mixed with deionized water at a solid-liquid ratio of 1:20 g / mL, firstly 0.8% of the mass of the dried kelp alginate lyase (enzyme activity of 200,000 U / g) is added, and enzymolysis is carried out at 35°C and pH 6.5 for 1 hour, and then 5% of the mass of the dried kelp papain (enzyme activity of 70,000 U / g) is added, and enzymolysis is carried out at 60°C and pH 7 for 60 minutes, the temperature is raised to 85°C for 15 minutes to inactivate the enzyme, and after cooling to room temperature, the filtrate is filtered, and the filtrate is spray-dried at 110°C to a water content of 3%, thereby obtaining the kelp extract.

[0040] Comparative Example 1 The specific implementation is the same as that of Example 4, except that the papain composition is replaced with papain.

[0041] Comparative Example 2 The specific implementation method is the same as that of Example 4, except that the ethanol solution in the preparation method of Cassia obtusifolia seed extract is replaced with water.

[0042] Comparative Example 3 The specific implementation is the same as that of Example 4, except that in the preparation method of the guava fruit extract, snail protease hydrolysis is first performed and then pectinase hydrolysis is performed.

[0043] Comparative Example 4 The specific implementation method is the same as that of Example 4, except that the composite microorganism in the preparation method of the sugarcane extract is composed of animal Bifidobacterium with a bacterial activity of 600 million CFU / g Bifidobacterium animalum CICC ® 6165 and the bacterial activity is 2 billion CFU / g fermented mucus lactobacillus Limosilactobacillus fermentum CICC ® 25124 was composed according to the mass ratio of 1:2.5, the fermentation temperature was 37°C, the pH value was 6, and the anaerobic fermentation was carried out for 3 days.

[0044] Comparative Example 5 The specific implementation method is the same as that of Example 4, except that the alginate lyase in the preparation process of kelp extract is replaced by pectin lyase with an enzyme activity of 150,000 U / g, the enzymatic hydrolysis temperature is 45°C, the pH value is 7.5, and the time is 2 hours; papain is replaced by trypsin with an enzyme activity of 60,000 U / g, the enzymatic hydrolysis temperature is 37°C, the pH value is 8, and the time is 80 minutes.

[0045] Test Example 1 pH stability The pH stability of cosmetics is an important indicator to measure their quality and safety. When the pH value of the product is stable within an appropriate range, it can reflect the compatibility between various ingredients and the stability of their efficacy.

[0046] The pH values ​​of the facial cleansers of Examples 4-6 and Comparative Examples 1-5 were measured by a pH meter when they were placed at room temperature for 0 h, 24 h, 48 h and 72 h. The specific results are shown in Table 1.

[0047] Table 1 pH values ​​of each group at different times

[0048] From the data in Table 1, it can be seen that the pH value of the cleansing milk prepared by Examples 4-6 of the present invention is stable, indicating that the various components of the present invention have good compatibility and strong efficacy stability. From the data of Example 4 and Comparative Examples 1-5, it can be seen that only adding papain and components obtained by different extraction methods can change the stability between the components.

[0049] Test Example 2 Skin irritation animal test A total of 40 healthy, adult guinea pigs weighing 250-350 g were selected and divided into 8 groups, with 5 in each group. There was no significant difference among the groups.

[0050] 24 hours before the experiment, the hair on both sides of the spine of the guinea pig's back was trimmed, about 3-5 cm on each side. 2 , expose the skin and be careful to avoid damaging the skin.

[0051] The guinea pig was fixed, and the facial cleansing lotions of Examples 4-6 and Comparative Examples 1-5 were evenly applied on the skin of one side of the hair removal area, and then covered and fixed with gauze and adhesive tape. The hair removal area on the other side was used as a blank control (no treatment), and the skin reactions were observed and recorded 24h, 48h and 72h after application. After each observation, the residual test substance was gently removed with warm water before observation.

[0052] Experimental results: No erythema or edema was observed in each group of Examples 4-6 and Comparative Examples 1-5, and there was no significant difference from the skin on the other side, i.e., no poisoning, indicating that the cleansing milks prepared in Examples 4-6 and Comparative Examples 1-5 were non-irritating and non-acutely toxic.

[0053] Test Example 3 Safety Experiment According to the requirements of the "Technical Specification for Safety of Cosmetics" (2015 edition), human skin patch tests were carried out on Examples 4-6 and Comparative Examples 1-5.

[0054] Subjects were screened according to the patch test criteria, and 90 people aged 20-45 years were selected and divided into 9 groups on average. There was no significant difference between the subjects in each group.

[0055] Human skin patch test: Gauze coated with the facial cleansing lotions of Examples 4-6 and Comparative Examples 1-5 was applied to the back of the test subject, and the gauze was gently pressed with the palm of the hand to evenly apply it on the skin for 48 hours, and a blank control (no human skin patch test) was used as a control. Skin reactions were observed according to the standards in Table 2 24 hours and 48 hours after application, and the observation results were recorded.

[0056] Table 2 Skin reaction grading standards for occlusive patch test

[0057] Test results: The skin reactions of the blank control, Examples 4-6 and Comparative Examples 1-5 were all negative, indicating that the cleansing milks of Examples 4-6 and Comparative Examples 1-5 had no adverse reactions and were safe.

[0058] Test Example 4 Acne removal, skin rejuvenation and brightening test According to the "Guidelines for the Treatment of Acne in China", subjects with acne graded as mild (Grade I) (only comedones) or moderate (Grade II) (with inflammatory papules) were enrolled in the test.

[0059] Exclusion criteria: pregnant or planning to become pregnant, using immunosuppressants or antihistamines, using topical products or cosmeceuticals to treat facial acne within 14 days, taking antibiotics to treat acne within 30 days, or suffering from severe systemic diseases and currently taking systemic medications, and allergic or sensitive to cosmetics / skin medicines.

[0060] The subjects were screened and 90 people aged 20-45 years were selected and divided into 9 groups with 10 people in each group. There was no significant difference between the subjects in each group.

[0061] Stop using products with similar effects 2 days before the test, and use facial cleanser once in the morning and once in the evening every day (after washing your hands, wet your face with warm water, put the cleanser in the palm of your hand, add a small amount of water, gently rub your hands and apply it evenly on your face, gently massage in circles with your fingertips, rinse the cleanser foam on your face thoroughly with warm water, and gently dry your face with a clean, soft towel), and use it for 28 consecutive days.

[0062] Facial tests are conducted on the 0th, 14th and 28th days (data are collected after cleansing the face with clean water and sitting quietly in a constant temperature and humidity environment for 30 minutes on the test day). Specialized testers count the total number of facial skin lesions (number of acne) on the subjects, measure the oil content and skin brightness, and evaluate the product's acne-removing, oil-removing, and skin-rejuvenating and brightening effects.

[0063] (1) Grease The forehead skin oil content was measured with a multifunctional skin tester. The higher the value, the higher the oil content on the skin surface. The average skin surface oil content of each group is shown in Table 3.

[0064] Average oil content (μg / cm 2 ) = the total amount of skin oil per person ÷ 10.

[0065] Table 3 Average skin oil content of different cleansers

[0066] From the data in Table 3, it can be seen that the facial cleansers prepared by using Examples 4-6 can significantly reduce the oil content in the skin and achieve a cleaning effect. From the data comparison between Example 4 and Comparative Example 1, it can be seen that the papain composition can significantly remove the oily components in the skin. From the data comparison between Example 4 and Comparative Examples 2-5, it can be seen that the extracts obtained by different extraction methods are added to the facial cleansers, which can affect the cleaning effect of the facial cleansers.

[0067] (2) Total number of skin lesions The average total number of skin lesions in each group on days 0, 14, and 28 were counted. The specific results are shown in Table 4.

[0068] Table 4 Average total number of skin lesions of different facial cleansers

[0069] As shown in Table 4, Examples 4-6 of the present invention can significantly reduce the amount of facial acne and achieve an acne-removing effect. Comparison of the data of Example 4 and Comparative Example 1 shows that the papain composition can reduce the inflammatory response of the skin more than papain, and has a more obvious acne-removing effect. Comparison of the data of Example 4 and Comparative Examples 2-5 shows that the extracts obtained by different extraction methods are added to the cleanser, which can affect the acne-removing effect of the cleanser.

[0070] (3) Brightness The skin color probe of the skin analyzer was used to detect and record the skin brightness ITA° values ​​of the forehead of each group of subjects on the 0th, 14th and 28th days, and the average value of ITA° was calculated. The specific results are shown in Table 5.

[0071] Table 5 Average ITA° values ​​of different cleansers

[0072] From the data in Table 5, it can be seen that Examples 4-6 of the present invention can significantly improve skin brightness and achieve whitening and brightening effects. From the data comparison between Example 4 and Comparative Example 1, it can be seen that the papain composition can improve skin brightness more than papain, and the whitening effect is more obvious. From the data comparison between Example 4 and Comparative Examples 2-5, it can be seen that the extracts obtained by different extraction methods are added to the cleansing milk, which can affect the whitening and brightening effects of the cleansing milk.

[0073] All groups had a positive effect on improving the brightness of the skin. Examples 1-3 of the present invention had obvious improvements after 14 days of use, which was better than that of comparative examples 2-8. Therefore, the composition of the present invention is highly effective and mild, and can achieve the effect of whitening, rejuvenating and brightening the skin in a short time.

[0074] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A papain composition, characterized in that The invention comprises the following components in parts by weight: 15-25 parts of lysozyme, 20-30 parts of superoxide dismutase, 25-35 parts of papain, 10-20 parts of lactoperoxidase, 3-7 parts of glucose oxidase, 5-15 parts of oxidoreductase and 20-30 parts of bromelain.

2. The method for preparing the papain composition according to claim 1, characterized in that: include: Lysozyme, superoxide dismutase, papain, lactoperoxidase, glucose oxidase, oxidoreductase and bromelain are mixed according to mass proportions to obtain a papain composition.

3. Use of the papain composition according to claim 1 in preparing facial cleansing cosmetics.

4. The use according to claim 3, characterized in that: The facial cleansing cosmetics include cleansing milk, cleansing cream, cleansing gel, cleansing foam or cleansing mask.

5. The use according to claim 4, characterized in that: The facial cleanser comprises the following components in parts by weight: 6-12 parts of papain composition, 0.5-1.5 parts of Cassia obtuse seed extract, 3-6 parts of guava fruit extract, 7-13 parts of sugarcane extract, 3-6 parts of kelp extract, 5-15 parts of camellia seed oil, 5-10 parts of squalane, 10-20 parts of surfactant, 10-20 parts of moisturizer, 1.5-4 parts of thickener, 0.1-0.5 parts of preservative and 40-60 parts of deionized water.

6. The use according to claim 5, characterized in that: The surfactant is one or more of cocamidopropyl betaine, sodium laureth sulfate, and sodium methyl cocoyl taurate.

7. The use according to claim 5, characterized in that: The moisturizing agent is one or more of allantoin, glycerol and pyrrolidone carboxylic acid.

8. The use according to claim 5, characterized in that: The thickener is one or more of hydroxyethyl cellulose, sodium acetate, PEG-150 distearate, and carbomer.

9. The use according to claim 5, characterized in that: The preservative is one or more of glyceryl caprylate, methylparaben, benzyl alcohol, and phenoxyethanol.

10. The use according to claim 5, characterized in that: The preparation method of the facial cleanser comprises: mixing and stirring a thickener, a moisturizer, a cassia seed extract, a guava fruit extract, a sugar cane extract and a kelp extract to obtain a water phase; mixing and stirring camellia seed oil and squalane to obtain an oil phase; mixing the oil phase and the water phase, adding a surfactant, a preservative and a papain composition, and adjusting the pH value to 6.5-7.5 to obtain the facial cleanser.

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