A papain composition, its preparation method and application

Abstract

The present invention provides a papain composition, a preparation method thereof and an application thereof, and the present invention relates to the technical field of cleansing cosmetics. The papain composition provided by the present invention comprises: lysozyme, superoxide dismutase, papain, lactoperoxidase, glucose oxidase, oxidoreductase and bromelain. The present invention uses the papain composition, together with Cassia obtusifolia seed extract, guava fruit extract, sugarcane extract and kelp extract, in cleansing cosmetics, which can deeply clean the skin, effectively remove oil and dirt; gently remove cutin, promote skin cell renewal, and make the skin smooth as silk; significantly remove acne and soothe skin inflammation, relieve skin discomfort; brighten the skin tone, improve dullness, and make the skin glow with natural luster; can promote skin metabolism, achieve the effect of skin rejuvenation, and decompose excess fat to achieve the effect of lipolysis, bringing all-round care and improvement to the skin.

Description

Technical Field

[0001] The present invention relates to the technical field of cleansing cosmetics, and particularly relates to a papain composition, a preparation method thereof and an application thereof. Background Art

[0002] Traditional cleansing cosmetics mainly rely on surfactants to achieve the cleansing function. However, many commonly used surfactants, such as sodium dodecyl sulfate, although having strong cleansing ability, often have some drawbacks. On the one hand, they may over-cleanse the skin, damage the skin's natural barrier function, resulting in problems such as dry skin, tightness, and sensitivity. Long-term use may exacerbate the water loss of the skin, affecting the skin's moisturizing ability and elasticity. On the other hand, some surfactants are irritating to the skin and may cause skin discomfort reactions, especially for people with sensitive skin, who are prone to symptoms such as redness and itching after use.

[0003] In recent years, enzymes, as a kind of natural biocatalyst, have gradually attracted attention in the application of cleansing cosmetics. Enzymes have high specificity and efficiency, and can catalyze specific chemical reactions under relatively mild conditions, thus achieving various functions such as cleansing and care. Compared with traditional chemical cleaners, enzymes are less irritating to the skin during the cleaning process and have better biocompatibility. For example, protease can decompose protein dirt on the skin surface, and lipase can decompose oil dirt. They can act on different types of dirt, and the action mode is relatively mild and not easy to cause excessive damage to the skin.

[0004] Papain is a plant protease extracted from papaya, which has unique properties and advantages. It can effectively decompose a variety of proteins, including keratin on the skin surface, protein components in dirt, and some protein substances related to skin problems, such as proteins secreted by certain bacteria that cause acne. Papain has activity in a relatively wide temperature and pH range, which enables it to adapt to different cosmetic formulation environments. However, using papain alone in cleansing cosmetics also faces some challenges, such as stability, compatibility with other ingredients, and enzymatic hydrolysis efficiency, which need to be solved through reasonable composition design. Therefore, developing a cleansing cosmetic based on a papain composition has important practical significance. It can not only utilize the cleansing and skin care advantages of papain but also overcome its limitations when used alone, meeting consumers' demands for highly efficient, mild, and multifunctional cleansing cosmetics, and having broad innovation and development space in the technical field of cleansing cosmetics. Summary of the Invention

[0005] In view of the above technical problems, the object of the present invention is to provide a papain composition, a preparation method and an application thereof. The papain composition provided by the present invention can be used in cleansing cosmetics and has multiple effects such as skin rejuvenation, bacteriostasis, acne removal, soothing, exfoliation, skin smoothing, brightening, cleaning, skin barrier remodeling, and fat dissolving.

[0006] In order to achieve the above object of the invention, the present invention provides the following technical solutions:

[0007] The present invention provides a papain composition, which comprises the following components in parts by weight: 15-25 parts of lysozyme, 20-30 parts of superoxide dismutase, 25-35 parts of papain, 10-20 parts of lactoperoxidase, 3-7 parts of glucose oxidase, 5-15 parts of oxidoreductase, and 20-30 parts of bromelain.

[0008] The present invention also provides a preparation method of the above papain composition, which comprises: mixing lysozyme, superoxide dismutase, papain, lactoperoxidase, glucose oxidase, oxidoreductase, and bromelain according to the mass parts to obtain the papain composition.

[0009] The present invention also provides an application of the above papain composition in the preparation of facial cleansing cosmetics.

[0010] Preferably, the facial cleansing cosmetics include facial cleanser, cleansing cream, cleansing gel, cleansing foam, and cleansing mask.

[0011] The present invention also provides a facial cleanser containing the above papain composition, which comprises the following components in parts by weight: 6-12 parts of papain composition, 0.5-1.5 parts of Cassia obtusifolia seed extract, 3-6 parts of guava fruit extract, 7-13 parts of sugarcane extract, 3-6 parts of kelp extract, 5-15 parts of camellia seed oil, 5-10 parts of squalane, 10-20 parts of surfactant, 10-20 parts of humectant, 1.5-4 parts of thickener, 0.1-0.5 parts of preservative, and 40-60 parts of deionized water.

[0012] Preferably, the surfactant is one or more of cocamidopropyl betaine, sodium lauryl polyether sulfate, and sodium methyl cocoyl taurate.

[0013] Preferably, the humectant is one or more of allantoin, glycerol, and pyrrolidone carboxylic acid.

[0014] Preferably, the thickener is one or more of hydroxyethyl cellulose, sodium acetate, PEG-150 distearate, and carbomer.

[0015] Preferably, the preservative is one or more of octanoic acid glyceride, methylparaben, benzyl alcohol, and phenoxyethanol.

[0016] The present invention also provides a method for preparing the above-mentioned facial cleanser, including: mixing and stirring a thickener, a humectant, Cassia obtusifolia seed extract, guava fruit extract, sugarcane extract, and kelp extract to obtain an aqueous phase; mixing and stirring camellia seed oil and squalane to obtain an oil phase; adding a surfactant, a preservative, and a papain composition to the mixture of the oil phase and the aqueous phase, and adjusting the pH value to 6.5 - 7.5 to obtain the facial cleanser.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] The present invention provides a papain composition, including: lysozyme, superoxide dismutase, papain, lactoperoxidase, glucose oxidase, oxidoreductase, and bromelain. The papain composition provided by the present invention can be used in cleansing cosmetics, which can deeply clean the skin, effectively remove oil and dirt; gently remove cutin, promote skin cell renewal, and make the skin smooth as silk; inhibit the growth of harmful bacteria and exert antibacterial efficacy; significantly remove acne and soothe skin inflammation, reducing skin discomfort; brighten the skin tone, improve dullness, and make the skin glow with natural luster; deeply nourish the skin, reshape the skin barrier function, and strengthen the skin's self-protection ability; also can promote skin metabolism, achieve skin rejuvenation effect, and decompose excess fat to achieve lipolysis effect, bringing all-round care and improvement to the skin. Detailed implementation manners

[0019] The present invention provides a papain composition, including the following components in parts by weight: 15 - 25 parts of lysozyme, 20 - 30 parts of superoxide dismutase, 25 - 35 parts of papain, 10 - 20 parts of lactoperoxidase, 3 - 7 parts of glucose oxidase, 5 - 15 parts of oxidoreductase, and 20 - 30 parts of bromelain.

[0020] The lysozyme of the present invention can enhance the skin's self-defense ability, adsorb and retain the moisture on the skin surface, prevent excessive loss of skin moisture, and has a synergistic effect when combined with the moisturizing components (allantoin, glycerol, pyrrolidone carboxylic acid) in the facial cleanser, enhancing the moisturizing effect of the entire cosmetic; superoxide dismutase has an antioxidant effect, preventing the damage of free radicals to the skin barrier and maintaining the integrity of the skin barrier; papain exfoliates the cutin, can decompose the protein dirt on the skin surface, and is more easily washed away by water, achieving the effect of effectively cleaning the skin. When combined with the plant-based components in the cosmetic, it has a soothing effect on the skin; lactoperoxidase can inhibit the growth of various bacteria, fungi and viruses, reducing skin inflammatory reactions; glucose oxidase has an antibacterial effect, can effectively remove the bacteria at the acne site, promote the recovery of acne, and can regulate the skin's microecological environment, enhancing the skin's barrier function. For example, in the facial cleanser, while cleaning the skin, it can ensure that beneficial microorganisms on the skin surface, such as Staphylococcus epidermidis, can continue to play their protective role on the skin; oxidoreductase has an antioxidant effect, can prevent the oxidation of easily oxidized components in the cosmetic, and stabilize the cosmetic components; bromelain can gently remove the cutin on the skin surface, make the skin smoother and more delicate, improve the skin color, and also has anti-inflammatory and soothing effects on the skin.

[0021] Each component of the papain composition of the present invention has a synergistic effect. After being used in cleansing cosmetics, it can achieve multiple effects such as skin rejuvenation, antibacterial, acne treatment, soothing, exfoliation, skin smoothing, brightening, skin barrier remodeling, and fat dissolution.

[0022] The present invention also provides a preparation method of the above-mentioned papain composition, including: mixing lysozyme, superoxide dismutase, papain, lactoperoxidase, glucose oxidase, oxidoreductase and bromelain according to mass parts to obtain the papain composition.

[0023] The present invention also provides the application of the above-mentioned papain composition in the preparation of facial cleansing cosmetics.

[0024] The facial cleansing cosmetics of the present invention include facial cleanser, cleansing cream, cleansing gel, cleansing foam and cleansing mask.

[0025] The present invention also provides a facial cleanser containing the above-mentioned papain composition, including the following components in parts by weight: 6 - 12 parts of papain composition, 0.5 - 1.5 parts of Cassia obtusifolia seed extract, 3 - 6 parts of guava fruit extract, 7 - 13 parts of sugarcane extract, 3 - 6 parts of kelp extract, 5 - 15 parts of camellia seed oil, 5 - 10 parts of squalane, 10 - 20 parts of surfactant, 10 - 20 parts of moisturizer, 1.5 - 4 parts of thickener, 0.1 - 0.5 part of preservative and 40 - 60 parts of deionized water.

[0026] The extract of Cassia obtusifolia seeds of the present invention has anti-inflammatory, antioxidant and anti-reddening effects; the extract of guava fruit has good anti-lipid peroxidation effect, delays skin aging, and has anti-inflammatory and astringent effects, and can also inhibit the activity of B-16 melanocytes and has whitening efficacy; the extract of sugarcane can adsorb and retain moisture on the skin surface, having a moisturizing effect; it removes the aged cutin on the skin surface, makes the skin smoother and more delicate, promotes skin metabolism, is beneficial to the absorption of subsequent skin care products, reduces skin inflammation, and remodels the skin barrier; the extract of kelp can adsorb dirt, oil and impurities on the skin surface, has good cleaning effect, and can form a moisturizing layer on the skin surface to improve the moisturizing effect, and has antioxidant and skin-soothing effects; camellia seed oil can dissolve oil dirt on the skin surface, such as the oil components in sebum and makeup residues, etc., and is more easily washed off by water, so as to achieve the purpose of cleaning the skin, and can reduce inflammation, relieve skin discomfort, and the antioxidant components contained can delay the skin aging process and keep the skin in a healthy and young state; squalane can blend well with the skin, is easily absorbed by the skin, and prevents the moisture inside the skin from evaporating through the epidermis, has a synergistic effect with moisturizers, and enhances the moisturizing effect of the skin.

[0027] The surfactant of the present invention is preferably one or more of cocamidopropyl betaine, sodium lauryl polyether sulfate, and sodium methyl cocoyl taurate; the moisturizer is preferably one or more of allantoin, glycerin, and pyrrolidone carboxylic acid; the thickener is preferably one or more of hydroxyethyl cellulose, sodium acetate, PEG-150 distearate, and carbomer; the preservative is preferably one or more of glycerol caprylate, methylparaben, benzyl alcohol, and phenoxyethanol.

[0028] Each component of the facial cleanser of the present invention has a synergistic effect, and has multiple effects such as skin rejuvenation, antibacterial, acne removal, soothing, exfoliating, skin smoothing, brightening, cleaning, skin barrier remodeling, and fat dissolution.

[0029] The present invention also provides a preparation method of the above-mentioned facial cleanser, including: mixing and stirring the thickener, moisturizer, extract of Cassia obtusifolia seeds, extract of guava fruit, extract of sugarcane, and extract of kelp to obtain an aqueous phase; mixing and stirring camellia seed oil and squalane to obtain an oil phase; adding the surfactant, preservative, and papain composition to the mixture of the oil phase and the aqueous phase, and adjusting the pH value to 6.5 - 7.5 to obtain the facial cleanser.

[0030] The usage method of the facial cleanser of the present invention: After washing hands, wet the face with warm water, put the facial cleanser in the palm, add a small amount of water, gently rub with both hands and then evenly apply on the face, gently massage in a circular motion with the fingertips, thoroughly rinse the facial cleanser foam on the face with warm water, and gently dry the face with a clean and soft towel.

[0031] In the present invention, unless otherwise specified, all raw material components are commercially available products well-known to those skilled in the art.

[0032] Next, the technical solutions of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without making creative efforts belong to the scope of protection of the present invention.

[0033] In the following embodiments, unless otherwise specified, all are conventional methods.

[0034] In the following embodiments, the materials, reagents, etc. used, unless otherwise specified, can be obtained from commercial channels.

[0035] In the following examples and comparative examples, lysozyme, superoxide dismutase, papain, lactoperoxidase, glucose oxidase, oxidoreductase, and bromelain were all purchased from Guangzhou Boshipai Biotechnology Co., Ltd., and the enzyme activities of lysozyme, superoxide dismutase, papain, lactoperoxidase, glucose oxidase, oxidoreductase, and bromelain were all 50 billion IU.

[0036] Example 1

[0037] Papain composition:

[0038] Mix 20 parts by weight of lysozyme, 25 parts by weight of superoxide dismutase, 30 parts by weight of papain, 15 parts by weight of lactoperoxidase, 5 parts by weight of glucose oxidase, 10 parts by weight of oxidoreductase, and 25 parts by weight of bromelain according to weight parts to obtain a papain composition.

[0039] Example 2

[0040] Papain composition:

[0041] Mix 15 parts by weight of lysozyme, 20 parts by weight of superoxide dismutase, 25 parts by weight of papain, 10 parts by weight of lactoperoxidase, 3 parts by weight of glucose oxidase, 5 parts by weight of oxidoreductase, and 20 parts by weight of bromelain according to weight parts to obtain a papain composition.

[0042] Example 3

[0043] Papain composition:

[0044] Mix 25 parts by weight of lysozyme, 30 parts by weight of superoxide dismutase, 35 parts by weight of papain, 20 parts by weight of lactoperoxidase, 7 parts by weight of glucose oxidase, 15 parts by weight of oxidoreductase and 30 parts by weight of bromelain according to parts by weight to obtain a papain composition.

[0045] Example 4

[0046] Papain composition facial cleanser:

[0047] (1) Put 45 parts by weight of deionized water into a container, turn on the stirrer, control the rotation speed at 250 r / min, slowly add 3 parts by weight of thickener to the deionized water until evenly dispersed to form a colloidal solution, and sequentially add 10 parts by weight of the papain composition in Example 1, 15 parts by weight of humectant, 1 part by weight of Cassia obtusifolia seed extract, 5 parts by weight of guava fruit extract, 10 parts by weight of sugarcane extract and 5 parts by weight of kelp extract until completely dissolved to obtain an aqueous phase;

[0048] (2) Stir 10 parts by weight of camellia seed oil and 8 parts by weight of squalane evenly at 55 °C to obtain an oil phase;

[0049] (3) Slowly drop the oil phase into the aqueous phase at a rotation speed of 400 r / min until a uniform emulsion is formed. Add 15 parts by weight of surfactant and 0.4 part by weight of preservative to the emulsion, adjust the pH value to 6.0, stop stirring, filter with a 100-mesh sieve to obtain a facial cleanser with uniform and delicate texture, sterilize it with a 30W ultraviolet lamp for 50 min, fill it, and package it;

[0050] Among them, the thickener consists of hydroxyethyl cellulose and sodium acetate in equal weights, the humectant consists of allantoin and glycerol in a weight ratio of 1:15, the surfactant is cocamidopropyl betaine, and the preservative consists of glycerol caprylate and methylparaben in equal weights;

[0051] The preparation method of Cassia obtusifolia seed extract is as follows: Crush Cassia obtusifolia seeds, pass through a 100-mesh sieve to obtain Cassia obtusifolia seed powder, mix it with an ethanol solution with a volume percentage content of 55% according to a solid-liquid ratio of 1:10 g / mL, perform ultrasonic extraction at 50 °C and 500 W for 45 min, filter, and concentrate the filtrate under reduced pressure at 45 °C and 5 kPa to a relative density of 1.2 to obtain Cassia obtusifolia seed extract;

[0052] The preparation method of guava fruit extract is as follows: fresh guava fruit is crushed by a wall breaking machine, mixed with deionized water at a solid-liquid ratio of 1:8 g / mL, firstly adding 0.3% pectinase (enzyme activity of 40,000 U / g) of the mass of guava fruit, enzymolysis for 2 hours at 50°C and pH 4, then adding 0.3% snail protease (enzyme activity of 1.5 U / g) of the mass of guava fruit, enzymolysis for 1.5 hours at 35°C and pH 4, heating to 85°C to inactivate the enzyme for 15 minutes, cooling to room temperature, filtering, spray drying the filtrate at 75°C to a water content of 5%, and obtaining the guava fruit extract;

[0053] The preparation method of sugarcane extract is as follows: fresh sugarcane is squeezed to obtain sugarcane juice and bagasse, the bagasse is crushed and passed through a 100-mesh sieve, mixed with water at a solid-liquid ratio of 1:10 g / mL, sugarcane juice is added, and a composite microorganism (containing 600 million CFU / g of Bifidobacterium adolescentis) is inoculated at a weight of 3‰ of the bagasse. Bifidobacterium adolensentis CICC ® 6177 and 2 billion Lactobacillus delbrueckii subsp. bulgaricus Lactobacillus delbrueckii subsp. bulgaricus CICC ® 6097 according to the mass ratio of 1:2.5) was anaerobically fermented at 40°C and pH 6 for 3 days, the fermentation liquid was filtered, and the filtrate was concentrated under reduced pressure at 60°C and 6 kPa to a relative density of 1.2 to obtain a sugarcane extract;

[0054] The preparation method of kelp extract is as follows: the dried kelp is crushed, passed through an 80-mesh sieve, mixed with deionized water at a solid-liquid ratio of 1:17 g / mL, firstly 0.6% of the mass of the dried kelp alginate lyase (enzyme activity of 150,000 U / g) is added, enzymolysis is carried out at 33°C and pH 6 for 2 hours, then 3% of the mass of the dried kelp papain (enzyme activity of 60,000 U / g) is added, enzymolysis is carried out at 55°C and pH 6 for 80 minutes, the temperature is raised to 85°C to inactivate the enzyme for 15 minutes, after cooling to room temperature, the filtrate is filtered, and the filtrate is spray-dried at 100°C to a water content of 5%, thereby obtaining the kelp extract.

[0055] Example 5

[0056] Papain Composition Cleanser

[0057] 40 parts by weight of deionized water are placed in a container, and an agitator is turned on with the speed controlled at 200 r / min. 1.5 parts by weight of a thickener is slowly added into the deionized water until it is evenly dispersed to form a colloidal solution. 6 parts by weight of the papain composition of Example 2, 10 parts by weight of a moisturizer, 0.5 parts by weight of an extract of Cassia obtusifolia seeds, 3 parts by weight of a guava fruit extract, 7 parts by weight of a sugarcane extract, and 3 parts by weight of a kelp extract are sequentially added into the colloidal solution until they are completely dissolved to obtain an aqueous phase.

[0058] (2) Stir 5 parts by weight of camellia seed oil and 5 parts by weight of squalane evenly at 50 °C to obtain an oil phase;

[0059] (3) Slowly add the oil phase to the water phase at a rotation speed of 300 r / min until a uniform emulsion is formed. Add 10 parts by weight of surfactant and 0.1 part by weight of preservative to the emulsion, adjust the pH value to 5.5, stop stirring, filter through an 80-mesh sieve to obtain a facial cleanser with uniform and delicate texture, sterilize it with a 30W ultraviolet lamp for 50 min, fill it, and package it;

[0060] Among them, the thickener is PEG-150 distearate, the moisturizer is composed of pyrrolidone carboxylic acid and glycerol in a mass ratio of 1:8, the surfactant is sodium lauryl polyether sulfate, and the preservative is phenoxyethanol;

[0061] The preparation method of Cassia obtusifolia seed extract is as follows: Crush Cassia obtusifolia seeds, pass through a 60-mesh sieve to obtain Cassia obtusifolia seed powder, mix it with an ethanol solution with a volume percentage of 50% according to a solid-liquid ratio of 1:8 g / mL, perform ultrasonic extraction at 40 °C and 450 W for 50 min, filter, and concentrate the filtrate under reduced pressure at 40 °C and 4.5 kPa to a relative density of 1.1 to obtain Cassia obtusifolia seed extract;

[0062] The preparation method of guava fruit extract is as follows: Crush fresh guava fruit, mix it with deionized water according to a solid-liquid ratio of 1:6 g / mL, first add pectinase (enzyme activity is 30,000 U / g) accounting for 0.2% of the mass of guava fruit, enzymatically hydrolyze at 45 °C and pH value of 3.5 for 3 h, then add snail protease (enzyme activity is 5,000 U / g) accounting for 0.1% of the mass of guava fruit, enzymatically hydrolyze at 30 °C and pH value of 3.5 for 2 h, raise the temperature to 85 °C to inactivate the enzyme for 15 min, cool to room temperature, filter, and spray-dry the filtrate at 70 °C until the water content is 8% to obtain guava fruit extract;

[0063] The preparation method of sugarcane extract is as follows: Press fresh sugarcane to obtain sugarcane juice and sugarcane bagasse. After crushing the sugarcane bagasse and passing it through an 80-mesh sieve, mix it with water according to a solid-liquid ratio of 1:8 g / mL, then add sugarcane juice, inoculate 2‰ of the weight of sugarcane bagasse with a composite microorganism (composed of Bifidobacterium adolescentis Bifidobacterium adolensentis CICC ® 6177 and Lactobacillus delbrueckii subsp. bulgaricus with a viable count of 1 billion CFU / g Lactobacillus delbrueckii subsp. bulgaricus CICC ® 6097 in a mass ratio of 1:2) and anaerobically ferment at 35 °C and pH value of 5.5 for 4 d, filter, and concentrate the filtrate under reduced pressure at 50 °C and 5.3 kPa to a relative density of 1.1 to obtain sugarcane extract;

[0064] The preparation method of kelp extract is as follows: crush dry kelp, sieve it through a 120-mesh sieve, mix it with deionized water according to a solid-liquid ratio of 1:15 g / mL, first add alginate lyase (enzyme activity is 100,000 U / g) accounting for 0.5% of the mass of dry kelp, enzymatically hydrolyze it at 30 °C and pH 5.5 for 3 h, then add papain (enzyme activity is 50,000 U / g) accounting for 2% of the mass of dry kelp, enzymatically hydrolyze it at 50 °C and pH 5 for 90 min, raise the temperature to 85 °C to inactivate the enzyme for 15 min, after cooling to room temperature, filter, and spray-dry the filtrate at 90 °C until the water content is 7% to obtain kelp extract.

[0065] Example 6

[0066] Papain composition facial cleanser

[0067] (1) Put 60 parts by weight of deionized water into a container, turn on the stirrer, control the rotation speed at 300 r / min, slowly add 4 parts by weight of thickener to the deionized water until evenly dispersed to form a colloidal solution, and successively add 12 parts by weight of the papain composition in Example 3, 15 parts by weight of humectant, 1 part by weight of Cassia obtusifolia seed extract, 5 parts by weight of guava fruit extract, 10 parts by weight of sugarcane extract and 5 parts by weight of kelp extract until completely dissolved to obtain an aqueous phase;

[0068] (2) Stir 10 parts by weight of camellia seed oil and 8 parts by weight of squalane evenly at 55 °C to obtain an oil phase;

[0069] (3) Slowly drop the oil phase into the aqueous phase at a rotation speed of 400 r / min until a uniform emulsion is formed, add 15 parts by weight of surfactant and 0.4 part by weight of preservative to the emulsion, adjust the pH value to 6.0, stop stirring, filter through a 100-mesh sieve to obtain a facial cleanser with uniform and delicate texture, sterilize it with a 30 W ultraviolet lamp for 50 min, fill and package;

[0070] Among them, the thickener is carbomer, the humectant is glycerol, the surfactant is sodium methyl cocoyl taurate, and the preservative is benzyl alcohol;

[0071] The preparation method of Cassia obtusifolia seed extract is as follows: crush Cassia obtusifolia seeds, sieve them through a 120-mesh sieve to obtain Cassia obtusifolia seed powder, mix it with an ethanol solution with a volume percentage content of 60% according to a solid-liquid ratio of 1:12 g / mL, ultrasonically extract it at 60 °C and 550 W for 40 min, filter, and concentrate the filtrate under reduced pressure at 50 °C and 5.5 kPa to a relative density of 1.3 to obtain Cassia obtusifolia seed extract;

[0072] The preparation method of guava fruit extract is as follows: Fresh guava fruit is crushed and mixed with deionized water according to a material-liquid ratio of 1:10 g / mL. First, pectinase (enzyme activity: 50,000 U / g) accounting for 0.5% of the mass of guava fruit is added, and enzymatic hydrolysis is carried out at 55 °C and a pH value of 4.5 for 1 h. Then, snail protease (enzyme activity: 20,000 U / g) accounting for 0.4% of the mass of guava fruit is added, and enzymatic hydrolysis is carried out at 40 °C and a pH value of 4.5 for 1 h. The temperature is raised to 85 °C to inactivate the enzyme for 15 min. After cooling to room temperature, filtration is carried out, and the filtrate is spray-dried at 80 °C until the water content is 3% to obtain guava fruit extract;

[0073] The preparation method of sugarcane extract is as follows: Fresh sugarcane is pressed to obtain sugarcane juice and sugarcane bagasse. After the sugarcane bagasse is crushed and sieved through a 120-mesh sieve, it is mixed with water according to a material-liquid ratio of 1:12 g / mL, and then sugarcane juice is added. A composite microorganism (composed of Bifidobacterium adolescentis with a viable count of 800 million CFU / g Bifidobacterium adolensentis CICC ® 6177 and Lactobacillus delbrueckii subsp. bulgaricus with a viable count of 3 billion CFU / g Lactobacillus delbrueckii subsp. bulgaricus CICC ® 6097 in a mass ratio of 1:3) is inoculated and anaerobically fermented at 42 °C and a pH value of 6.5 for 4 d. Filtration is carried out, and the filtrate is concentrated under reduced pressure at 65 °C and 6.5 kPa to a relative density of 1.3 to obtain sugarcane extract;

[0074] The preparation method of kelp extract is as follows: Dried kelp is crushed and sieved through a 60-mesh sieve, and then mixed with deionized water according to a material-liquid ratio of 1:20 g / mL. First, alginate lyase (enzyme activity: 200,000 U / g) accounting for 0.8% of the mass of dried kelp is added, and enzymatic hydrolysis is carried out at 35 °C and a pH value of 6.5 for 1 h. Then, papain (enzyme activity: 70,000 U / g) accounting for 5% of the mass of dried kelp is added, and enzymatic hydrolysis is carried out at 60 °C and a pH value of 7 for 60 min. The temperature is raised to 85 °C to inactivate the enzyme for 15 min. After cooling to room temperature, filtration is carried out, and the filtrate is spray-dried at 110 °C until the water content is 3% to obtain kelp extract.

[0075] Comparative Example 1

[0076] The specific implementation method is the same as that of Example 4, except that the papain composition is replaced by papain.

[0077] Comparative Example 2

[0078] The specific implementation method is the same as that of Example 4, except that the ethanol solution in the preparation method of Cassia obtusifolia seed extract is replaced by water.

[0079] Comparative Example 3

[0080] The specific implementation method is the same as that of Example 4, except that in the preparation method of guava fruit extract, snail protease enzymolysis is carried out first, and then pectinase enzymolysis is carried out.

[0081] Comparative Example 4

[0082] The specific implementation method is the same as that of Example 4, except that in the preparation method of sugarcane extract, the complex microorganism consists of Bifidobacterium animalis with a viable count of 600 million CFU / g Bifidobacterium animalis CICC ® 6165 and Lactobacillus mucosae fermentum with a viable count of 2 billion CFU / g Limosilactobacillus fermentum CICC ® 25124, and they are composed according to a mass ratio of 1:2.5. The fermentation temperature is 37 °C, the pH value is 6, and anaerobic fermentation is carried out for 3 days.

[0083] Comparative Example 5

[0084] The specific implementation method is the same as that of Example 4, except that the alginate lyase in the preparation process of kelp extract is replaced with pectin lyase with an enzyme activity of 150,000 U / g. The enzymolysis temperature is 45 °C, the pH value is 7.5, and the time is 2 h; papain is replaced with trypsin with an enzyme activity of 60,000 U / g. The enzymolysis temperature is 37 °C, the pH value is 8, and the time is 80 min.

[0085] Test Example 1

[0086] pH value stability

[0087] The pH value stability of cosmetics is an important indicator to measure their quality and safety. When the product pH value is stable within an appropriate range, it can reflect the compatibility and efficacy stability among various components.

[0088] The pH values of the facial cleansers of Examples 4-6 and Comparative Examples 1-5 were measured by a pH meter at 0 h, 24 h, 48 h, and 72 h at room temperature. The specific results are shown in Table 1.

[0089] Table 1 pH value results of each group at different times

[0090]

[0091] It can be seen from the data in Table 1 that the pH values of the facial cleansers prepared by Examples 4-6 of the present invention are stable, indicating good compatibility and strong efficacy stability among various components of the present invention. From the data of Example 4 and Comparative Examples 1-5, it can be seen that only adding papain and components obtained by different extraction methods can change the stability among components.

[0092] Test Example 2

[0093] Animal experiment on skin irritation

[0094] Select 40 healthy, adult guinea pigs weighing 250 - 350 g, divided into 8 groups with 5 guinea pigs in each group, and there are no significant differences among the groups.

[0095] 24 h before the experiment, cut the hair on both sides of the spine on the back of the guinea pigs, about 3 - 5 cm on each side 2 , expose the skin, and pay attention to avoiding skin damage.

[0096] Fix the guinea pigs, evenly apply the facial cleansers of Examples 4 - 6 and Comparative Examples 1 - 5 on one side of the depilated area of the skin, and then cover and fix with gauze and adhesive tape. The other side of the depilated area serves as a blank control (without treatment), and observe and record the skin reactions at 24 h, 48 h, and 72 h after application respectively. After each observation, gently remove the remaining test substances with warm water and then conduct the observation.

[0097] Experimental results: No erythema and edema occurred in each group of Examples 4 - 6 and Comparative Examples 1 - 5, and there was no obvious difference from the skin on the other side, that is, no poisoning phenomenon, indicating that the facial cleansers prepared in Examples 4 - 6 and Comparative Examples 1 - 5 have no irritation and acute toxicity.

[0098] Test Example 3

[0099] Safety experiment

[0100] Conduct human skin patch tests on Examples 4 - 6 and Comparative Examples 1 - 5 in accordance with the requirements of the "Technical Specifications for Cosmetics Safety" (2015 Edition).

[0101] Select 90 subjects according to the patch test standard, aged 20 - 45 years old, and evenly divide them into 9 groups, with no significant differences among the subjects in each group.

[0102] Human skin patch test: Apply the gauze coated with the facial cleansers of Examples 4 - 6 and Comparative Examples 1 - 5 on the back of the subjects respectively, gently press with the palm to make it evenly adhere to the skin, and keep it for 48 h, using the blank control (without human skin patch) as the control. Observe the skin reactions according to the standard in Table 2 at 24 h and 48 h after application, and record the observation results.

[0103] Table 2 Grading standard for skin reactions in skin closed patch test

[0104]

[0105] Test results: The skin reactions of the blank control, Examples 4 - 6, and Comparative Examples 1 - 5 are all negative, indicating that the facial cleansers of Examples 4 - 6 and Comparative Examples 1 - 5 have no adverse reactions and are safe.

[0106] Test Example 4

[0107] Acne removal, skin rejuvenation and brightening test

[0108] Subjects with acne graded as mild (Grade I) (only comedones) and moderate (Grade II) (with inflammatory papules) according to the "Chinese Acne Treatment Guidelines" were enrolled in the test.

[0109] Exclusion criteria: Pregnant or planning to be pregnant, using immunosuppressants or antihistamines, using topical products or pharmaceutical cosmetics for facial acne treatment within 14 days, taking antibiotics for acne treatment within 30 days, or having severe systemic diseases and currently using systemic medications, and those allergic or sensitive to cosmetics / skin medications.

[0110] Ninety subjects were screened, aged 20 - 45 years old, and evenly divided into 9 groups with 10 people in each group. There were no significant differences among the subjects in each group.

[0111] Stop using products with similar effects 2 days before the test. Use the facial cleanser once in the morning and once in the evening every day (After washing hands, wet the face with warm water, put the facial cleanser in the palm of the hand, add a small amount of water, gently rub with both hands and then evenly apply on the face, gently massage in a circular motion with the fingertips, thoroughly rinse the facial cleanser foam on the face with warm water, and gently dry the face with a clean and soft towel), and use it continuously for 28 days.

[0112] Facial-related tests were conducted on days 0, 14, and 28 (After cleansing the face with clean water on the test day, sit quietly in an environment with constant temperature and humidity for 30 minutes and then collect data), and a specialized tester counted the total number of facial skin lesions (number of acne) of the subjects, measured the oil content and skin brightness, so as to evaluate the acne-removing, oil-removing, skin-softening and brightening effects of the product.

[0113] (1) Oil

[0114] Measure the skin oil on the forehead with a multi-functional skin tester. The higher the value, the higher the oil content on the skin surface. The average skin surface oil content of each group is shown in Table 3.

[0115] Average oil content (μg / cm 2 ) = sum of skin oil of each person ÷ 10.

[0116] Table 3 Average skin oil content of different facial cleansers

[0117]

[0118] As can be seen from the data in Table 3, the facial cleansers prepared in Examples 4-6 can significantly reduce the oil content in the skin, achieving a cleaning effect. By comparing the data of Example 4 and Comparative Example 1, it can be seen that the papain composition can significantly remove the oily components in the skin. By comparing the data of Example 4 and Comparative Examples 2-5, it can be seen that adding the extracts obtained by different extraction methods to the facial cleanser can affect the cleaning effect of the facial cleanser.

[0119] (2)Total number of skin lesions

[0120] The average total number of skin lesions in each group on days 0, 14, and 28 was counted, and the specific results are shown in Table 4.

[0121] Table 4 Average total number of skin lesions of different facial cleansers

[0122]

[0123] As can be seen from the results in Table 4, Examples 4-6 of the present invention can significantly reduce the number of facial acne, achieving an acne-removing effect. By comparing the data of Example 4 and Comparative Example 1, it can be seen that the papain composition can reduce the skin inflammatory reaction more than papain, and the acne-removing effect is more obvious. By comparing the data of Example 4 and Comparative Examples 2-5, it can be seen that adding the extracts obtained by different extraction methods to the facial cleanser can affect the acne-removing efficacy of the facial cleanser.

[0124] (3)Brightness

[0125] The skin brightness ITA° values of the foreheads of the faces of the subjects in each group were detected and recorded on days 0, 14, and 28 using the skin color probe of the skin analyzer, and the average value of ITA° was calculated. The specific results are shown in Table 5.

[0126] Table 5 Average ITA° values of different facial cleansers

[0127]

[0128] As can be seen from the data in Table 5, Examples 4-6 of the present invention can significantly improve the skin brightness, achieving a whitening and brightening effect. By comparing the data of Example 4 and Comparative Example 1, it can be seen that the papain composition can improve the skin brightness more than papain, and the whitening effect is more obvious. By comparing the data of Example 4 and Comparative Examples 2-5, it can be seen that adding the extracts obtained by different extraction methods to the facial cleanser can affect the whitening and brightening efficacy of the facial cleanser.

[0129] Each group has a positive effect on improving the skin brightness. Examples 1-3 of the present invention have shown obvious improvement after 14 days of use, and the effect is better than that of Comparative Examples 2-8. Therefore, the composition of the present invention is highly efficient, mild, and can achieve the effect of whitening, tenderizing, and brightening the skin in a short time.

[0130] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and modifications can be made, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A facial cleanser containing a papain composition, characterized in that, It comprises the following components in parts by weight: 6 - 12 parts of papain composition, 0.5 - 1.5 parts of Cassia obtusifolia seed extract, 3 - 6 parts of guava fruit extract, 7 - 13 parts of sugarcane extract, 3 - 6 parts of kelp extract, 5 - 15 parts of camellia seed oil, 5 - 10 parts of squalane, 10 - 20 parts of surfactant, 10 - 20 parts of humectant, 1.5 - 4 parts of thickener, 0.1 - 0.5 parts of preservative and 40 - 60 parts of deionized water; The papain composition comprises the following components in parts by weight: 15 - 25 parts of lysozyme, 20 - 30 parts of superoxide dismutase, 25 - 35 parts of papain, 10 - 20 parts of lactoperoxidase, 3 - 7 parts of glucose oxidase, 5 - 15 parts of oxidoreductase and 20 - 30 parts of bromelain; The preparation method of the Cassia obtusifolia seed extract is as follows: crush the Cassia obtusifolia seeds, pass through a 100 - mesh sieve to obtain Cassia obtusifolia seed powder, mix it with an ethanol solution with a volume percentage of 55% according to a solid - liquid ratio of 1:10 g / mL, carry out ultrasonic extraction at 50°C and 500 W for 45 min, filter, and concentrate the filtrate under reduced pressure at 45°C and 5 kPa to a relative density of 1.2 to obtain the Cassia obtusifolia seed extract; The preparation method of the guava fruit extract is as follows: break fresh guava fruits with a blender, mix them with deionized water according to a solid - liquid ratio of 1:8 g / mL, first add pectinase accounting for 0.3% of the mass of the guava fruits, carry out enzymatic hydrolysis at 50°C and a pH value of 4 for 2 h, then add snail protease accounting for 0.3% of the mass of the guava fruits, carry out enzymatic hydrolysis at 35°C and a pH value of 4 for 1.5 h, raise the temperature to 85°C to inactivate the enzyme for 15 min, after cooling to room temperature, filter, and spray - dry the filtrate at 75°C until the water content is 5% to obtain the guava fruit extract; the enzyme activity of the pectinase is 40,000 U / g, and the enzyme activity of the snail protease is 1,500 U / g; The preparation method of the sugarcane extract is as follows: Fresh sugarcane is pressed to obtain sugarcane juice and sugarcane bagasse. After the sugarcane bagasse is crushed and passed through a 100-mesh sieve, it is mixed with water according to a solid-liquid ratio of 1:10 g / mL, and then sugarcane juice is added. A compound microorganism accounting for 3‰ of the weight of the sugarcane bagasse is inoculated and anaerobically fermented at 40 °C and a pH value of 6 for 3 days. The fermentation broth is filtered, and the filtrate is concentrated under reduced pressure at 60 °C and 6 kPa to a relative density of 1.2 to obtain the sugarcane extract; the compound microorganism consists of Bifidobacterium adolensentis CICC ® 6177 with a viable count of 600 million CFU / g and Lactobacillus delbrueckii subsp. bulgaricus CICC ® 6097 in a mass ratio of 1:2.5; The preparation method of the kelp extract is as follows: crush dry kelp, pass through an 80 - mesh sieve, mix it with deionized water according to a solid - liquid ratio of 1:17 g / mL, first add alginate lyase accounting for 0.6% of the mass of the dry kelp, carry out enzymatic hydrolysis at 33°C and a pH value of 6 for 2 h, then add papain accounting for 3% of the mass of the dry kelp, carry out enzymatic hydrolysis at 55°C and a pH value of 6 for 80 min, raise the temperature to 85°C to inactivate the enzyme for 15 min, after cooling to room temperature, filter, and spray - dry the filtrate at 100°C until the water content is 5% to obtain the kelp extract; the enzyme activity of the alginate lyase is 150,000 U / g, and the enzyme activity of the papain is 60,000 U / g.

2. The facial cleanser according to claim 1, characterized in that, The preparation method of the papain composition includes: mixing lysozyme, superoxide dismutase, papain, lactoperoxidase, glucose oxidase, oxidoreductase and bromelain according to parts by weight to obtain the papain composition.

3. The facial cleanser according to claim 1, characterized in that, The surfactant is one or more of cocoamidopropyl betaine, sodium lauryl polyether sulfate, and sodium methyl cocoyl taurate.

4. The facial cleanser according to claim 1, characterized in that, The humectant is one or more of allantoin, glycerol, and pyrrolidone carboxylic acid.

5. The facial cleanser according to claim 1, wherein The thickener is one or more of hydroxyethyl cellulose, sodium acetate, PEG-150 distearate, and carbomer.

6. The facial cleanser according to claim 1, wherein The preservative is one or more of glycerol caprylate, methylparaben, benzyl alcohol, and phenoxyethanol.

7. A preparation method of the facial cleanser according to any one of claims 1-6, characterized in that, It includes: Mix and stir the thickener, humectant, Cassia obtusifolia seed extract, guava fruit extract, sugarcane extract, and kelp extract to obtain an aqueous phase; mix and stir camellia seed oil and squalane to obtain an oil phase; after mixing the oil phase and the aqueous phase, add a surfactant, a preservative, and a papain composition, and adjust the pH value to 6.5 - 7.5 to obtain a facial cleanser.

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