Composition of bionic intercellular lipid and application thereof

Abstract

The invention relates to the technical field of novel daily chemical materials, and particularly discloses a bionic intercellular lipid composition and application thereof. The composition is prepared from ceramide E, phytosterol oleate and vegetable oil with the carbon chain length of C16-24. Wherein the ceramide E can interact with vegetable oil with the carbon chain length of C16-24 to construct a lipid bilayer, the lipid bilayer is further converted into a layered liquid crystal structure by virtue of the self-assembly characteristic of the lipid bilayer, and the phytosterol oleate maintains the long-term stability of the liquid crystal structure through a spatial regulation mechanism. The long-acting stable liquid crystal structure not only can efficiently simulate intercellular lipid, but also can keep good binding efficiency with hydrogen bonds between skins; the expression of key enzymes can be remarkably promoted, and moisture between the skins can be locked for a long time. The bionic intercellular lipid composition is applied to the technical field of skin care and moisture preservation, not only can show excellent long-acting water replenishing and moisture preservation effects, but also can play a role in repairing damaged skin.

Description

Technical Field

[0001] The present application relates to the technical field of daily chemical new materials, and in particular to a composition of bionic intercellular lipids and its application. Background Art

[0002] Intercellular lipids, also known as structural lipids, usually exist in the intercellular space in the form of bimolecular liquid crystal structures. In the "brick-gray structure" of the skin, they play a key role similar to the "mortar" between bricks. Intercellular lipids are synthesized by spinous cells and are mainly distributed in the cytoplasm in the form of lamellar bodies (Orland bodies); in the process of spinous cells migrating upward and differentiating, the lamellar bodies gradually move toward the cell periphery and fuse with the cell membrane, and are finally released into the intercellular space through the exocytosis mechanism. The components of intercellular lipids include ceramide, fatty acids, and cholesterol. It connects with apoptotic keratinocytes with the help of intercellular desmosome proteins to jointly build a brick-cement structure. This structure greatly enhances the adhesion between cells and provides a solid material support for the stratum corneum to have stable and tough physical properties. This special structure composed of intercellular lipids can effectively prevent the loss of water and electrolytes on the one hand, and resist the invasion of harmful substances on the other hand, playing an indispensable role in maintaining the homeostasis of the skin's internal environment.

[0003] When the skin barrier is damaged, the physiological lipids between cells will show a large amount of loss, causing the brick wall structure of the skin to collapse. In this process, the arrangement of keratinocytes tends to be loose, which in turn induces a series of problems such as dry skin and desquamation. Many studies have shown that the occurrence and development of skin diseases such as psoriasis and atopic dermatitis are closely related to the loss of intercellular lipids and the imbalance of the proportion of related components in intercellular lipids. In view of the above-mentioned damaged skin barrier, the existing technology mainly achieves the repair of the skin barrier by simulating bionic sebum or directly supplementing lipids. Bionic sebum technology generally promotes the adhesion of sebum to the surface of the skin, thereby forming a layer of oil film, which reduces the rate of water loss by means of this layer of oil film, thereby exerting a moisturizing effect, but the moisturizing effect is relatively short-lived. In the way of directly supplementing lipids, ceramide is a common supplementary ingredient. However, in the actual development of cosmetics, ceramide faces many challenges. On the one hand, ceramide has poor solubility and is difficult to add to a high concentration in cosmetic formulas; on the other hand, ceramide has poor transdermal permeability and low bioavailability.

[0004] In order to improve the bioavailability of ceramide, researchers often use emerging technologies such as nanotechnology to improve the transdermal absorption efficiency of ceramide and its retention in the skin. However, the use of microfluidics to prepare nanoparticle-sized bionic intercellular lipids is not suitable for baby products. In summary, the development of a product with wide applicability, which can effectively lock in moisture and effectively repair damaged skin barriers, has become a key issue that needs to be urgently addressed in the current cosmetics field. Summary of the invention

[0005] In view of this, the purpose of the present application is to provide a composition of bionic intercellular lipids and its application, which is used to solve the problems of narrow application range and poor water-locking effect of existing water-locking repair products.

[0006] In order to achieve the above technical objectives, the present application provides a composition of biomimetic intercellular lipids, comprising the following components in parts by weight:

[0007] Ceramide E, 1-2 parts;

[0008] Phytosteryl oleate, 1-2 parts;

[0009] Vegetable oil with carbon chain length of C16-24, 1 to 2 parts.

[0010] Furthermore, the phytosterol oleates include β-sitosterol, stigmasterol, campesterol, and brassicasterol.

[0011] Furthermore, the vegetable oil with a carbon chain length of C16-24 is at least one of meadowfoam seed oil, olive oil, linseed oil, and tea seed oil.

[0012] Furthermore, the plant oil with a carbon chain length of C16-24 is meadowfoam seed oil; the mass ratio of ceramide E, phytosteryl oleate, and meadowfoam seed oil is 2:1:1.

[0013] The present application provides a method for preparing a composition of biomimetic intercellular lipids, comprising the following preparation steps:

[0014] Ceramide E, phytosterol oleate and vegetable oil with a carbon chain length of C16-24 are mixed and stirred evenly at 88-90° C. to obtain a composition of biomimetic intercellular lipids.

[0015] The present application provides an application of a composition of bionic intercellular lipids for preparing skin care and moisturizing products.

[0016] Furthermore, the skin care moisturizing product includes the following components in percentage by mass:

[0017] The composition of biomimetic intercellular lipids is 0.1% to 20%;

[0018] Moisturizer 0.1%~30%;

[0019] Thickener 0.1%~5.0%;

[0020] Emulsifier 0.2%~4.0%;

[0021] Emollients 0.1% to 3.0%;

[0022] pH adjuster 0.1%~1.0%;

[0023] Preservatives 0.1% to 0.5%;

[0024] The balance is water.

[0025] Furthermore, the skin care moisturizing product includes the following components in percentage by mass:

[0026] Composition of biomimetic intercellular lipids 12%;

[0027] Moisturizer 4%;

[0028] Thickener 0.3%;

[0029] Emulsifier 2.8%;

[0030] Emollient 3%;

[0031] pH adjuster 0.7%;

[0032] Preservative 0.4%;

[0033] The balance is water.

[0034] Furthermore, the moisturizer includes one or more of glycerin, propylene glycol, and 1,2-pentanediol; the thickener includes one or more of sodium carboxymethyl cellulose, hydroxyethyl cellulose, xanthan gum, and carrageenan; the emulsifier includes one or more of cetearyl olivate, sorbitan olivate, sorbitan monostearate, and polysorbate 80; and the emollient includes one or more of caprylic / capric triglyceride and glyceryl tri(ethylhexanoate).

[0035] Furthermore, the moisturizing agent consists of glycerol and propylene glycol, and the mass ratio of glycerol to propylene glycol is 1:3.

[0036] In summary, the present application provides a composition of bionic intercellular lipids, which is constructed from ceramide E, phytosterol oleate, and vegetable oil with a carbon chain length of C16-24. The two amide groups in ceramide E perpendicular to the hydrocarbon chain axis interact with the vegetable oil with a carbon chain length of C16-24 to construct a lipid bilayer, which is converted into a lamellar liquid crystal structure by virtue of its self-assembly characteristics, while phytosterol oleate substances mainly maintain the long-term stability of the liquid crystal structure through a spatial structure regulation mechanism. This long-lasting and stable liquid crystal structure can not only efficiently simulate intercellular lipids, but also show good binding efficiency with hydrogen bonds between the skin; it can also significantly promote the expression of three key enzymes to achieve long-term moisture locking between the skin. Applying this bionic intercellular lipid composition to the field of skin care and moisturizing products can not only show excellent long-term hydration and moisturizing effects, but also play a positive role in the repair of damaged skin.

[0037] Compared with the prior art, the bionic intercellular lipid composition of the present application has significant advantages. First, it does not require the addition of nanostructures and has a wide range of applications. It can be used in adult washing and care products as well as infant products. Second, its water-locking principle is different from that of the prior art. Most of the prior art allows sebum to adhere to the surface of the skin to form an oil film that reduces water loss to exert moisturizing effects, and the moisturizing time is short; while the composition of the present application mainly achieves water lock by simulating intercellular lipids and forming hydrogen bonds with water, which can achieve long-term and stable water lock effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0039] Figure 1 This is a schematic diagram showing the changes in the promoting effect of the bionic intercellular lipid composition provided in the examples and comparative examples of the present application on the expression of cholesterol synthase mRNA at different times.

[0040] Figure 2 This is a schematic diagram of the changes in the promoting effect of the bionic intercellular lipid composition provided in the examples and comparative examples of the present application on the expression of fatty acid synthase mRNA at different times.

[0041] Figure 3 This is a schematic diagram showing the changes in the promoting effect of the composition of bionic intercellular lipids provided in the examples and comparative examples of the present application on the expression of ceramide synthase mRNA at different times.

[0042] Figure 4 Schematic diagram of the change in water locking rate of the bionic intercellular lipid composition provided in the examples and comparative examples of the present application at different times.

[0043] Figure 5 A schematic diagram of the change in water content of the stratum corneum of the skin before and after using a skin care moisturizing product provided for the application examples of this application and the comparative application examples.

[0044] Figure 6 A schematic diagram of the change in transepidermal water loss rate of the skin before and after using a skin care moisturizing product provided for the application examples of this application and the comparative application examples.

[0045] Figure 7 The tissue morphology test characterization diagram and immunofluorescence protein intensity diagram provided for the BC group, NC group, PC group, comparative application example 1 and application example 1 of this application.

[0046] Figure 8 Comparison chart of immunofluorescence protein content provided for the BC group, NC group, PC group, comparative application example 1 and application example 1 of this application. DETAILED DESCRIPTION

[0047] The technical solutions of the embodiments of the present application will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the specification of the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection requested by the present application.

[0048] The sources of all raw materials in the present invention are not particularly limited and can be purchased on the market or prepared according to conventional methods known to those skilled in the art.

[0049] The present application embodiment provides a composition of biomimetic intercellular lipids, comprising the following components in parts by weight:

[0050] Ceramide E, 1-2 parts;

[0051] Phytosteryl oleate, 1-2 parts;

[0052] Vegetable oil with carbon chain length of C16-24, 1 to 2 parts.

[0053] It should be noted that ceramide E contains long-chain hydrocarbon groups and amide groups. The long-chain hydrocarbon groups have strong hydrophobicity; the amide groups (-CONH-) have certain polarity and can form hydrogen bonds with water molecules. The amide groups are perpendicular to the hydrocarbon chain axis. This specific spatial orientation can combine with vegetable oils with carbon chain lengths of C16-24 to form a lipid bilayer.

[0054] Plant oils with carbon chain lengths of C16-24 (such as white meadowfoam seed oil) are mainly composed of a variety of fatty acids, which have both hydrophobic hydrocarbon chain parts and relatively hydrophilic polar groups such as carboxyl groups. The hydrophobic hydrocarbon part can aggregate with the long-chain hydrocarbon group of ceramide E, reducing the contact area with the surrounding water molecules and reducing the free energy of the system; the polar groups in the plant oil can form hydrogen bonds with the two amide groups in ceramide E that are perpendicular to the hydrocarbon chain axis. Driven by the hydrophobic effect and hydrogen bonding, ceramide E and plant oil will spontaneously arrange to form a lipid bilayer structure. In this bilayer, the hydrophobic hydrocarbon chain parts of ceramide E and white meadowfoam seed oil face the inside of the bilayer, while the polar groups face the outside of the bilayer and interact with the surrounding water environment. In this process, the lipid bilayer formed is not chaotic, but in an orderly arranged state. The orderly arranged lipid bilayer then further self-assembles to form a layered structure under various intermolecular interaction forces (such as van der Waals forces, hydrogen bonds, etc.). The molecular arrangement in this layered structure has a certain orientation order, but it is not completely regular and rigid like a crystal. Instead, it has a certain fluidity and flexibility, thus exhibiting the characteristics of liquid crystals, that is, forming a layered liquid crystal structure.

[0055] Phytosterol oleate has a tetracyclic steroid nucleus structure similar to cholesterol, and there is a hydroxyl group at the C-3 position of the steroid nucleus. This structure makes phytosterol oleate both hydrophobic and hydrophilic. Under the conditions of heating (88℃~90℃) and mixing and stirring, phytosterol oleate with hydrophobic and hydrophilic properties is inserted into the lipid bilayer activated by high temperature, filling the gap between ceramide E and plant oil (such as white meadowfoam seed oil) molecules; because the structure of phytosterol oleate has a certain similarity with lipid molecules, phytosterol oleate can interact well with the phospholipid molecules in the lipid bilayer, making the structure of the lipid bilayer more compact and orderly, reducing the fluidity and freedom of lipid molecules, and thus stabilizing the lamellar liquid crystal structure.

[0056] Due to the characteristics of phytosterol oleate and lipid bilayer, when phytosterol oleate is inserted into the lipid bilayer, the lipid bilayer formed has a special flow control mechanism. In the appropriate temperature range (20°C~40°C), the lipid bilayer in this embodiment can be smoothed by the inserted phytosterol oleate, which is specifically manifested in preventing lipid molecules from excessively moving due to high temperature (temperature greater than 40°C); at the same time, in a relatively low temperature state (temperature less than 20°C), it can prevent lipid molecules from being too rigid and causing the destruction of the liquid crystal structure, so that the fluidity of the lipid bilayer is maintained within a suitable range, which is conducive to the stable existence of the lamellar liquid crystal structure. The appropriate temperature range and relatively low temperature state here are determined according to the material ratio and characteristics of the composition, and can be determined by simple experimental detection.

[0057] In addition, the hydroxyl groups of phytosterol oleate can form additional hydrogen bonds or other weak interactions with the amide groups of ceramide E and polar groups of plant oils (such as meadowfoam seed oil), further enhancing the binding force between molecules in the lipid bilayer, thereby improving the stability of the lamellar liquid crystal structure.

[0058] In some embodiments, the phytosterol oleate includes β-sitosterol, stigmasterol, campesterol, and rapeseed sterol. Specifically, the phytosterol oleate can be purchased, and when used, it is necessary to confirm that the purchased phytosterol oleate includes at least four sterols, namely β-sitosterol, stigmasterol, campesterol, and rapeseed sterol.

[0059] In the specific examples of the present application, the phytosterol oleate used is purchased from Advanced Organic Materials SA with the trade name Advasterol Ester IP PC G.

[0060] In some embodiments, the vegetable oil with a carbon chain length of C16-24 is at least one of meadowfoam seed oil, olive oil, linseed oil, and tea seed oil.

[0061] It should be noted that the length of the fatty chain in the intercellular lipids of the skin is mainly between 16 and 24 carbon atoms, and long-chain fatty acids can make the vegetable oil have better stability and antioxidant properties. For example, in the above embodiment, the carbon chain length of white meadowfoam seed oil is mainly distributed in the C20-22 range; olive oil, linseed oil, tea seed oil and other vegetable oils rich in long-chain fatty acids have carbon chain lengths in the C16-18 range.

[0062] In a specific implementation, the vegetable oil with a carbon chain length of C16-24 is meadowfoam seed oil; the mass ratio of ceramide E, phytosteryl oleate, and meadowfoam seed oil is 2:1:1.

[0063] In a specific embodiment of the present application, a method for preparing a composition of biomimetic intercellular lipids is further provided, comprising the following preparation steps:

[0064] Ceramide E, phytosterol oleate and vegetable oil with a carbon chain length of C16-24 are mixed, and stirred evenly at 88° C. to 90° C. to obtain a composition of bionic intercellular lipids.

[0065] In a specific embodiment of the present application, there is further provided an application of a composition of biomimetic intercellular lipids for preparing skin care and moisturizing products.

[0066] In some embodiments, the skin care moisturizing product includes the following components in percentage by weight:

[0067] The composition of biomimetic intercellular lipids is 0.1% to 20%;

[0068] Moisturizer 0.1%~30%;

[0069] Thickener 0.1%~5.0%;

[0070] Emulsifier 0.2%~4.0%;

[0071] Emollients 0.1% to 3.0%;

[0072] pH adjuster 0.1%~1.0%;

[0073] Preservatives 0.1% to 0.5%;

[0074] The balance is water;

[0075] Preferably, the balance is deionized water.

[0076] In a specific embodiment, the skin care moisturizing product includes the following components in percentage by weight:

[0077] Composition of biomimetic intercellular lipids 12%;

[0078] Moisturizer 4%;

[0079] Thickener 0.3%;

[0080] Emulsifier 2.8%;

[0081] Emollient 3%;

[0082] pH adjuster 0.7%;

[0083] Preservative 0.4%;

[0084] The balance is water;

[0085] Preferably, the balance is deionized water.

[0086] In some embodiments, the humectant includes one or more of glycerin, propylene glycol, and 1,2-pentanediol; the thickener includes one or more of sodium carboxymethyl cellulose, hydroxyethyl cellulose, xanthan gum, and carrageenan; the emulsifier includes one or more of cetearyl olivate, sorbitan olivate, sorbitan monostearate, and polysorbate 80; the emollient includes one or more of caprylic / capric triglyceride and glyceryl tri(ethylhexanoate).

[0087] In some embodiments, the moisturizer is composed of glycerol and propylene glycol, and the mass ratio of glycerol to propylene glycol is 1:3; the thickener is ammonium acryloyldimethyltaurate / VP copolymer; the emulsifier includes cetearyl olivate and sorbitan olivate; and the emollient is triethylhexanoin.

[0088] The present application further provides the following reference examples to describe the present application. It should be noted that these examples are merely illustrative and do not limit the present invention in any way.

[0089] Example 1

[0090] This embodiment provides a composition of biomimetic intercellular lipids, comprising the following components in parts by weight:

[0091] 1 part of ceramide E; 1 part of phytosteryl oleate; 1 part of vegetable oil with a carbon chain length of C16-24. Among them, phytosteryl oleate was purchased from Advanced Organic Materials SA; and the vegetable oil with a carbon chain length of C16-24 was meadowfoam seed oil.

[0092] This embodiment also provides a method for preparing a biomimetic intercellular lipid composition, the steps of which are as follows: ceramide E, phytosteryl oleate, and meadowfoam seed oil are mixed, and stirred evenly at 88 to 90° C. to obtain a biomimetic intercellular lipid composition.

[0093] Embodiments 2 to 4

[0094] The difference from Example 1 is that the proportions of the components added are different, see Table 1 for details.

[0095] Comparative Examples 1 to 3

[0096] The difference from Example 1 is that the added components and proportions are different, see Table 1 for details.

[0097] Table 1. Compositions and parameters of biomimetic intercellular lipids

[0098]

[0099] The bionic intercellular lipid compositions prepared in the above Examples 1 to 4 and Comparative Examples 1 to 3 were tested for their effects and performance, and the test indicators included the mRNA expression of key synthases and the water-locking capacity test.

[0100] Detection 1: mRNA expression test of key synthases

[0101] Experimental method: Human keratinocytes were inoculated into 6-well plates and placed in an incubator (37°C, 5% CO2) for overnight incubation. When the plating rate of human keratinocytes in the 6-well plates reached 50%, 1 mL of the composition of biomimetic intercellular lipids prepared in the embodiment and the comparative example and 2 mL of culture medium were added to the six-well plates respectively; blank control groups (with physiological saline replacing the composition of biomimetic intercellular lipids) were set up respectively, and then the six-well plates were placed in an incubator (37°C, 5% CO2) for continued culture for 24 h and 48 h. After the culture was completed, human keratinocytes were washed with PBS and collected by centrifugation, and 1 mL of AG RNAex ProReagent was added to the human keratinocytes to lyse the cells, and the lysed cells were sequenced, and the mRNA expression levels of cholesterol synthesis rate-limiting enzymes (HMGCoA reductase), fatty acid synthase (FAS) and ceramide synthase (STP1) in human keratinocyte cultures were measured. The measured data are shown in Table 2, and the following is drawn based on the data in the table: Figures 1 to 3 The bar graph shown was prepared and data analysis was performed.

[0102] Table 2. Increase rate of mRNA expression of three synthases at different times

[0103]

[0104] Figure 1 A schematic diagram showing the changes in mRNA expression of the rate-limiting enzyme in cholesterol synthesis at different culture times; Figure 2 A schematic diagram showing the changes in mRNA expression of fatty acid synthase at different culture times; Figure 3 The schematic diagram of the change of mRNA expression of ceramide synthase at different culture times is shown. It can be seen from the figure that compared with the blank group, the mRNA expression of the three key synthases in the components of the composition with biomimetic intercellular lipids added showed a significant increase. Among them, Examples 3 and 4 have the best effect on promoting the mRNA expression of the rate-limiting enzyme in cholesterol synthesis; Examples 2 and 3 have the best effect on promoting the mRNA expression of fatty acid synthase; Examples 1 and 3 are most effective in promoting the mRNA expression of ceramide synthase.

[0105] It can be seen from these data that the biomimetic intercellular lipid composition provided in the above embodiments has improved the comprehensive regulation of the mRNA expression of three key enzymes, cholesterol synthase, fatty acid synthase and ceramide synthase. Further based on the effects of the composition components in the embodiments, the proportion of each component in the composition can be further accurately adjusted based on the various embodiments provided in this application and the contents disclosed therein, so as to achieve orderly regulation of the expression of the above key enzymes.

[0106] Test 2: Water lock test

[0107] Take 20g (accurate to 0.0001g) of the composition of bionic intercellular lipids prepared in the above embodiments and comparative examples and evenly apply it on the rapid filter paper until the filter paper is completely wetted. Place the moistened filter paper in a constant temperature and humidity machine at a temperature of 40±2°C and a relative humidity of 65±5% for 8 hours. During this process, the filter paper is taken out and weighed at four time points, namely the 1st, 2nd, 4th, and 8th hour after the start, and the binding force of the bionic intercellular lipid composition to water at this time point is calculated. In this experiment, the moisture retention rate is used to indicate the binding force of the composition with water. The higher the moisture retention rate, the stronger the binding force of the composition with water. The experimental results are shown in Table 3, and the following is plotted based on the data in Table 3. Figure 4 In the experiment, three parallel experiments were conducted for each embodiment and comparative example and the average value was taken, and a clean and completely wetted rapid filter paper was used as a blank group.

[0108] Table 3. Moisturizing efficiency at different times

[0109]

[0110] Figure 4 The schematic diagram of the change of the moisturizing rate of the bionic intercellular lipid composition provided in the examples and comparative examples at different times. It can be seen from the figure that the moisturizing rates shown in Examples 1 to 4 are all better than those in the comparative examples, and the water loss rate of Example 3 is the slowest, and the moisturizing and water-locking effect is the best.

[0111] Application Example 1

[0112] This application example provides a skin care moisturizing product containing a bionic intercellular lipid composition, and its components and mass percentages are detailed in Table 4. When preparing the product, firstly, component B is heated to 88-90°C and stirred thoroughly until the components are evenly mixed; then component A is added and stirred continuously until the system reaches a homogeneous state; finally, component C is added and stirred again until the system reaches a homogeneous state to obtain a skin care moisturizing product.

[0113] Table 4. Ingredients and parameters of skin care moisturizing products containing biomimetic intercellular lipid compositions

[0114]

[0115] Comparative application example 1

[0116] The difference from Application Example 1 is that the composition of biomimetic intercellular lipids is replaced by an equal amount of water.

[0117] In order to comprehensively evaluate the performance of skin care and moisturizing products, a series of experimental tests were carried out on the skin care and moisturizing products prepared in the application examples and comparative application examples, including stratum corneum water content test, transepidermal water loss test and stratum corneum repair test.

[0118] The experimental environment conditions are set as follows: the temperature is maintained at 20℃~22℃, and the humidity is controlled at 40%~60%. The experiment recruited more than 30 subjects, and the subjects were required to use the test samples continuously for 14 days. The specific test instructions are as follows: First, clean the face and inner side of the arm of the subject daily, take an appropriate amount of the corresponding product and apply it evenly on the face and inner side of the arm, and gently massage until the product is completely absorbed. The frequency of use is once in the morning and evening every day, and it is used continuously for 14 days. During the entire trial, strict behavioral restrictions are imposed on the subjects, including: prohibiting the use of any preparations with soothing and repairing effects; prohibiting the ingestion of preparations that affect the results of repair and soothing tests through drips, injections, oral administration or other methods; at the same time, the subjects are required to focus on indoor activities as much as possible, avoid long-term exposure to outdoor light environments, and finally collect data. This part of the requirements will be used for screening to ensure the accuracy and reliability of the experimental results and minimize the interference of external factors on the test results.

[0119] Test 1: Stratum corneum moisture content test

[0120] Before use and on the 14th day after use, the stratum corneum moisture content of the subjects' inner arms was tested 2 hours after the subjects used the skin care moisturizing products. The test results are shown in Table 5. Figure 5 The bar graph is shown, and the change rate of skin moisture content is calculated according to the following formula: Skin moisture content change rate (%) = (moisture content D after using the product) 14 -Moisture content before using the product (D0) / Moisture content before using the product (D0) × 100%.

[0121] Table 5. Change rate of skin moisture content

[0122]

[0123] Figure 5 The figure is a graph showing the change in moisture content of the stratum corneum of the skin before and after using the skin care moisturizing product. It can be seen from the figure that the moisture content of the stratum corneum of the skin of the subjects who used the skin care moisturizing product prepared in the application example increased by 35.12%; the moisture content of the stratum corneum of the skin of the subjects who used the skin care moisturizing product prepared in the comparative application example increased by 6.9%. Compared with the comparative application example, the moisturizing and water-locking ability of the skin care moisturizing product provided in this application is far superior to that of the comparative application example.

[0124] Test 2: Transepidermal water loss

[0125] Before use and on the 14th day after use, the subjects' faces were tested for transepidermal water loss rate 2 hours after using the skin care moisturizing products. The test results are shown in Table 6. Figure 6The bar graph is shown, and the change rate of skin transepidermal water loss rate is calculated according to the following formula: Skin transepidermal water loss rate change rate (%) = (transepidermal water loss rate after using the product D 14 -Transepidermal water loss rate before using the product (D0) / Transepidermal water loss rate before using the product (D0) × 100%.

[0126] Table 6. Changes in skin transepidermal water loss

[0127]

[0128] Figure 6 The figure is a graph showing the change in transepidermal water loss rate of the stratum corneum of the skin before and after using the skin care moisturizing product. It can be seen from the figure that the transepidermal water loss rate of the stratum corneum of the skin of the subjects who used the skin care moisturizing product prepared in the comparative application example was reduced by 4.8%; the transepidermal water loss rate of the stratum corneum of the skin of the subjects who used the skin care moisturizing product prepared in the application example was reduced by 18.25%. It can be seen that the composition of bionic intercellular lipids prepared in the example can effectively reduce the transepidermal water loss rate of the skin and enhance the water retention effect of the skin.

[0129] Test 3: Repair test of damaged stratum corneum

[0130] In order to further compare the repairing effects of the application examples and the comparative application examples on the damaged stratum corneum. In this experiment, a blank control group (BC), a positive control group (NC), a positive control group (PC), and a sample group were set up. The parameter settings of each group are shown in Table 7. Poly I:C+LPS was used to stimulate the barrier weakening-3D epidermal skin model (EpiKutis®), and the changes in tissue morphology and filaggrin (FLG) content were detected to evaluate the repair of the skin stratum corneum tissue structure and the promotion of related protein expression by the bionic intercellular lipid example samples. The results can be seen in Figures 7-8 and Table 8.

[0131] Table 7. Setting parameters for damaged stratum corneum repair test

[0132]

[0133] Figure 8The tissue morphology test characterization diagrams and immunofluorescent protein intensity diagrams of the BC group, NC group, PC group, comparative application example 1 and application example 1 are shown. From the tissue morphology test characterization diagram, the skin tissue structure of the BC group is relatively regular, and the thickness of the stratum corneum is moderate; there are slight changes in some areas of the NC group (such as the yellow circled part of the NC group in the figure), and the uniformity of the tissue structure is slightly insufficient. The reason is that the NC group is immune stimulated, and the stratum corneum shrinks to varying degrees due to the stimulation; the tissue morphology of the PC group is between the first two, because dexamethasone can alleviate the stimulating effect of the immunostimulant on the stratum corneum to a certain extent, but dexamethasone cannot restore the stratum corneum to its initial state (such as the BC group); the skin tissue structure of comparative application example 1 is better than that of the PC group, but there is still uneven thickness of the stratum corneum; and the tissue structure of application example 1 is more compact, orderly and uniform, which shows that application example 1 can more effectively alleviate the stimulating effect of the immunostimulant on the stratum corneum, thereby improving the skin tissue.

[0134] The intensity of immunofluorescent protein can be used to indicate the strength of skin barrier function. Figure 7 In the immunofluorescence protein intensity diagram shown in FIG. 1 , the FLG content can be seen in the green fluorescent part of the diagram; more intuitively, the FLG content can be seen in Table 8 and the prepared from Table 8 Figure 8 . As can be seen from the figure, the green fluorescence signal of the BC group is weak, indicating that the FLG content is low and the skin barrier function is relatively weak; the fluorescence signal of the NC group is not strong either, which also reflects that the FLG content is limited and the barrier function needs to be improved; the fluorescence signal of the PC group is enhanced compared with the NC group, but the overall green fluorescence signal is still weak; compared with the green fluorescence intensity of Application Example 1, the green fluorescence signal intensity of Application Example 1 is significantly stronger than that of Application Example 1, which indicates that the skin care and moisturizing products in Application Example 1 can significantly increase the FLG content, thereby effectively enhancing the skin's barrier function. It can be seen that Application Example 1 has obvious advantages in promoting the increase of FLG content and improving skin tissue structure compared with other groups.

[0135] Table 8. FLG content and improvement rate under different parameter conditions

[0136]

[0137] Note: IOD stands for integrated optical density, which is Figure 7 The green fluorescence signal intensity in the IOD value corresponds to the green fluorescence signal intensity in the FLG content. The larger the IOD value, the higher the FLG content.

[0138] The above are only preferred embodiments of the present application and are not intended to limit the present invention. Although the present application has been described in detail with reference to the examples, those skilled in the art can still modify the technical solutions recorded in the aforementioned examples or make equivalent substitutions for some of the technical features therein. However, any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. A composition of biomimetic intercellular lipids, characterized in that: The composition includes the following components in parts by weight: Ceramide E, 1-2 parts; Phytosteryl oleate, 1-2 parts; Vegetable oil with carbon chain length of C16-24, 1 to 2 parts.

2. The composition of biomimetic intercellular lipids according to claim 1, characterized in that: The phytosterol oleates include β-sitosterol, stigmasterol, campesterol, and brassicasterol.

3. The composition of biomimetic intercellular lipids according to claim 1, characterized in that: The vegetable oil with a carbon chain length of C16-24 is at least one of meadowfoam seed oil, olive oil, linseed oil and tea seed oil.

4. The composition of biomimetic intercellular lipids according to claim 3, characterized in that: The vegetable oil with a carbon chain length of C16-24 is meadowfoam seed oil; the mass ratio of ceramide E, phytosteryl oleate and meadowfoam seed oil is 2:1:

1.

5. A method for preparing the biomimetic intercellular lipid composition according to any one of claims 1 to 4, characterized in that: The method comprises the following preparation steps: Ceramide E, phytosterol oleate and vegetable oil with a carbon chain length of C16-24 are mixed and stirred evenly at 88-90° C. to obtain a composition of biomimetic intercellular lipids.

6. Use of the composition of biomimetic intercellular lipids according to any one of claims 1 to 4, characterized in that: Used to prepare skin care moisturizing products.

7. The use of the composition of biomimetic intercellular lipids according to claim 6, characterized in that: The skin care moisturizing product comprises the following components in percentage by mass: The composition of biomimetic intercellular lipids is 0.1% to 20%; Moisturizer 0.1%~30%; Thickener 0.1%~5.0%; Emulsifier 0.2%~4.0%; Emollients 0.1% to 3.0%; pH adjuster 0.1%~1.0%; Preservatives 0.1% to 0.5%; The balance is water.

8. The use of the composition of biomimetic intercellular lipids according to claim 7, characterized in that: The skin care moisturizing product comprises the following components in percentage by mass: Composition of biomimetic intercellular lipids 12%; Moisturizer 4%; Thickener 0.3%; Emulsifier 2.8%; Emollient 3%; pH adjuster 0.7%; Preservative 0.4%; The balance is water.

9. The use of the composition of biomimetic intercellular lipids according to claim 6, characterized in that: The humectant includes one or more of glycerin, propylene glycol, and 1,2-pentanediol; The thickener includes one or more of sodium carboxymethyl cellulose, hydroxyethyl cellulose, xanthan gum, and carrageenan; The emulsifier includes one or more of cetearyl olivate, sorbitan olivate, sorbitan monostearate, and polysorbate 80; The emollient includes one or more of caprylic / capric triglyceride and tri(ethylhexanoin)glyceryl.

10. The use of the composition of biomimetic intercellular lipids according to claim 6, characterized in that: The moisturizing agent consists of glycerol and propylene glycol, and the mass ratio of glycerol to propylene glycol is 1:3.

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