Ionic liquid anti-allergy soothing composition as well as preparation method and application thereof

Abstract

The invention discloses an ionic liquid anti-allergy soothing composition as well as a preparation method and application thereof. The preparation method comprises the following steps: carefully selecting selaginella tamariscina, rhodiola rosea and fructus forsythiae, degrading by adopting cellulase to obtain a primary degradation product, inoculating a strain for biological fermentation to further obtain a small-molecule active substance, and fully extracting the active substance by adopting ionic liquid combined with an ultrahigh pressure technology to obtain the ionic liquid anti-allergy soothing composition. The preparation process of the ionic liquid anti-allergy soothing composition is green and natural, environment-friendly and high in active matter extraction rate. Tests prove that when the composition is applied to a cosmetic matrix, good anti-allergy and soothing effects can be achieved.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and in particular to an ionic liquid anti-allergic soothing composition and a preparation method and application thereof. Background Art

[0002] Sensitive skin often manifests itself as itching, tingling and burning sensations, which are related to damaged skin barrier function, skin neuroimmune disorders and imbalance of skin microecology.

[0003] The anti-allergic and soothing active ingredients commonly used in skin care products include plant-derived ingredients and non-plant-derived ingredients. Among them, plant-derived ingredients are more widely used in skin care products due to their easy acquisition, simple production process, significant efficacy, mild effect and few side effects.

[0004] For the extraction of active ingredients from natural plants, commonly used solvents include water, ethanol, methanol, petroleum ether, ether, benzene, chloroform, ethyl acetate, n-butanol, acetone, etc., which can be selected according to the chemical properties of the target ingredients. However, many organic solvents are highly toxic and volatile, and are expensive. With the continuous advancement of science and technology, green and environmentally friendly extraction technologies are gaining more and more attention in the development of natural plant resources. Deep eutectic solvents (DES) are a type of ionic liquid in supramolecular assembly technology. They are two-component or three-component eutectic mixtures composed of a certain stoichiometric ratio of hydrogen bond acceptors (such as quaternary ammonium salts) and hydrogen bond donors (such as amides, carboxylic acids, and polyols). They have the advantages of being adjustable and designable. As a new, environmentally friendly extraction medium, they are gradually becoming a popular choice in the field of natural plant extraction. Summary of the invention

[0005] The purpose of the present invention is to provide an ionic liquid anti-allergic soothing composition, which uses a low eutectic solvent acceptable in the cosmetics field to extract natural plant active ingredients, and applies it in skin care products to achieve good anti-allergic and soothing effects.

[0006] The specific technical solutions of the present invention are as follows:

[0007] An ionic liquid anti-allergic soothing composition contains Selaginella tamariscina extract, Rhodiola rosea root extract and Forsythia suspensa fruit extract.

[0008] The Selaginella described in the present invention is the dried whole herb of Selaginella of the Selaginaceae family. Selaginella is also known as the grass of nine dead resurrections. It is pungent and flat in nature and enters the liver and heart meridians. It has the medicinal effects of anti-inflammatory, analgesic, blood circulation and menstruation. It is reported that it can treat trauma, especially those hemorrhagic traumas. After the occurrence, fresh Selaginella can be directly used for external application, which can play a role in rapid hemostasis and analgesia. The main active ingredients of Selaginella include Selaginene, Selaginol A, Selaginol B, Selaginone and Selaginella lactone. These ingredients have multiple pharmacological effects, such as anti-oxidation, anti-inflammatory, antibacterial and anti-tumor.

[0009] The Rhodiola rosea described in the present invention is the dried root of Rhodiola rosea of ​​the Crassulaceae family. It tastes sweet and bitter, is neutral in nature, and enters the lung and heart meridians. It has the medicinal effects of invigorating qi and promoting blood circulation, unblocking meridians and relieving asthma. The main active ingredients of the Rhodiola rosea root include salidroside, rosavirin, tyrosol, etc. These ingredients can help the human body cope with stress and fatigue, and have multiple effects such as anti-oxidation, anti-inflammatory and immune regulation.

[0010] The forsythia suspensa described in the present invention is the mature fruit of the shrub forsythia suspensa of the genus forsythia of the family Oleaceae. "Compendium of Materia Medica" records that forsythia suspensa has the medicinal effects of clearing away heat and detoxifying, reducing swelling and dispersing nodules, and is one of the traditional Chinese medicines commonly used in clinical practice in China. The oil content of forsythia suspensa seeds is 25%-33%, rich in oleic acid and linoleic acid that are easily absorbed and digested by the human body, and has an oily aroma. The main active ingredients of forsythia suspensa fruits include phenylethanol glycosides, lignans, phenolic acids, flavonoids, terpenes and volatile oils. Among them, phenylethanol glycosides and lignans are the components with relatively abundant content in forsythia suspensa fruits, and have pharmacological activities such as anti-inflammatory, antibacterial, antiviral and antioxidant.

[0011] The embodiment of the present invention also discloses a preparation process of the ionic liquid anti-allergic soothing composition, comprising the following steps:

[0012] Step 1: Take fresh Selaginella whole herb, Rhodiola rosea root, and Forsythia suspensa fruit, dry them in the shade, crush and sieve them to obtain plant powder;

[0013] Step 2: Mix plant powder, water and cellulase, perform enzymolysis under heating and stirring, and inactivate the enzyme at high temperature to obtain primary degradation products;

[0014] Step 3: inoculating a mixed strain of lactobacillus and Candida utilis into the primary degradation product, fermenting and culturing, and obtaining a fermentation material;

[0015] Step 4: mix lactobionic acid, betaine and butanediol in a molar ratio of 1-10:1-10:50-100, heat and dissolve to obtain an ionic liquid; mix the fermentation material and the ionic liquid in a mass ratio of 1:1-10, perform ultrahigh pressure assisted extraction and enhanced treatment, let stand for aging, centrifuge and obtain the supernatant to obtain the ionic liquid anti-allergic soothing composition.

[0016] In some embodiments, the mass ratio of the whole herb of Selaginella, the root of Rhodiola rosea, and the fruit of Forsythia suspensa is 1-10:1-10:1-10.

[0017] In some embodiments, the sieving is through a 30-50 mesh sieve.

[0018] In some embodiments, the activity of the cellulase is 100,000-200,000 U / g; the mass ratio of the plant powder, water, and cellulase is 1:10-20:0.01-0.1.

[0019] In some of the embodiments, the enzymatic hydrolysis conditions are: pH 4.0-5.0, temperature 45-50° C., hydrolysis for 3-5 hours, and then boiling water bath for 20 minutes to inactivate the enzyme.

[0020] In some embodiments, the mass ratio of the mixed bacteria to the primary degradation product is 1:100-200; the number of viable lactobacilli in the mixed bacteria is ≥10 9 cfu / g, viable count of Candida utilis ≥10 9 cfu / g.

[0021] In some of the embodiments, the fermentation culture condition is sealed fermentation at 30-35° C. for 1-3 days.

[0022] In some of the embodiments, the ultrahigh pressure assisted extraction condition is 100-200 MPa ultrahigh pressure assisted extraction for 1-3 minutes.

[0023] The ionic liquid anti-allergic soothing composition prepared in the embodiment of the present invention can be used as an active ingredient in cosmetic formulas, and its addition amount can be 1-10wt%. The cosmetics can be in the form of creams, lotions, facial masks, essences, facial cleansers, etc.

[0024] The technical solution provided by the embodiment of the present invention has the following beneficial effects: the present invention selects Selaginella, Rhodiola rosea, and Forsythia suspensa, first uses cellulase to degrade them, obtains primary degradation products, then inoculates strains for biological fermentation, further obtains small molecule active substances, and then uses ionic liquid combined with ultra-high pressure technology to fully extract the active substances, thereby obtaining an ionic liquid anti-allergic soothing composition. The whole process is green and natural, environmentally friendly, and has a high extraction rate of active substances. Experiments have confirmed that the application of it in a cosmetic matrix can achieve good anti-allergic and soothing effects. DETAILED DESCRIPTION

[0025] The present invention discloses an ionic liquid anti-allergic soothing composition. Those skilled in the art can refer to the content of this article and appropriately improve the process parameters to achieve it. It is particularly important to point out that all similar substitutions and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the methods and applications described herein without departing from the content, spirit and scope of the present invention to implement and apply the technology of the present invention.

[0026] The raw materials and reagents used in the ionic liquid anti-allergic soothing composition provided by the present invention can be purchased from the market.

[0027] The present invention will be further described below in conjunction with embodiments:

[0028] Example 1

[0029] An ionic liquid anti-allergic soothing composition, the preparation process of which comprises the following steps:

[0030] Step 1, taking fresh Selaginella whole herb, Rhodiola rosea root, and Forsythia suspensa fruit, wherein the mass ratio of the Selaginella whole herb, Rhodiola rosea root, and Forsythia suspensa fruit is 1:10:1; drying in the shade, and crushing through a 50-mesh sieve to obtain plant powder;

[0031] Step 2: Mix plant powder, water and cellulase in a mass ratio of 1:10:0.1, wherein the activity of the cellulase is 100,000 U / g; adjust the pH to 5.0, heat to 45° C., perform enzymolysis for 5 hours under stirring, and inactivate the enzyme in a boiling water bath for 20 minutes to obtain a primary degradation product;

[0032] Step 3: Inoculate the primary degradation product with a mixed strain of lactobacillus and Candida utilis, wherein the mass ratio of the mixed strain to the primary degradation product is 1:100; the number of viable lactobacilli in the mixed strain is ≥10 9 cfu / g, viable count of Candida utilis ≥10 9 cfu / g; sealed and fermented at 35°C for 1 day to obtain fermented material;

[0033] Step 4. Mix lactic acid, betaine and butanediol in a molar ratio of 10:10:100, heat and dissolve to obtain an ionic liquid; mix the fermentation material and the ionic liquid in a mass ratio of 1:1, perform 200MPa ultra-high pressure assisted extraction and intensive treatment for 1 minute, let it stand for 3 days, centrifuge at 3000rpm for 30 minutes, and take the supernatant to obtain the ionic liquid anti-allergic soothing composition.

[0034] Example 2

[0035] An ionic liquid anti-allergic soothing composition, the preparation process of which comprises the following steps:

[0036] Step 1, taking fresh Selaginella whole herb, Rhodiola rosea root, and Forsythia suspensa fruit, wherein the mass ratio of the Selaginella whole herb, Rhodiola rosea root, and Forsythia suspensa fruit is 10:1:10; drying in the shade, and crushing through a 30-mesh sieve to obtain plant powder;

[0037] Step 2: Mix plant powder, water and cellulase in a mass ratio of 1:20:0.01, wherein the activity of the cellulase is 200,000 U / g; adjust the pH to 4.0, heat to 50° C., perform enzymolysis for 3 hours under stirring, and inactivate the enzyme in a boiling water bath for 20 minutes to obtain a primary degradation product;

[0038] Step 3: Inoculate the primary degradation product with a mixed strain of lactobacillus and Candida utilis, wherein the mass ratio of the mixed strain to the primary degradation product is 1:200; the number of viable lactobacilli in the mixed strain is ≥10 9 cfu / g, viable count of Candida utilis ≥10 9 cfu / g; sealed and fermented for 3 days at 30-35°C to obtain fermented material;

[0039] Step 4. Mix lactic acid, betaine and butanediol in a molar ratio of 1:1:50, heat and dissolve to obtain an ionic liquid; mix the fermentation material and the ionic liquid in a mass ratio of 1:10, perform 100 MPa ultra-high pressure assisted extraction and intensive treatment for 3 minutes, let it stand for 1 day, centrifuge at 3000 rpm for 30 minutes, and take the supernatant to obtain the ionic liquid anti-allergic soothing composition.

[0040] Example 3

[0041] An ionic liquid anti-allergic soothing composition, the preparation process of which comprises the following steps:

[0042] Step 1, taking fresh Selaginella whole herb, Rhodiola rosea root, and Forsythia suspensa fruit, wherein the mass ratio of the Selaginella whole herb, Rhodiola rosea root, and Forsythia suspensa fruit is 6:5:3; drying in the shade, and crushing through a 40-mesh sieve to obtain plant powder;

[0043] Step 2: Mix plant powder, water and cellulase in a mass ratio of 1:15:0.05, wherein the activity of the cellulase is 200,000 U / g; adjust the pH to 4.5, heat to 48° C., perform enzymolysis for 4 hours under stirring, and inactivate the enzyme in a boiling water bath for 20 minutes to obtain a primary degradation product;

[0044] Step 3: Inoculate the primary degradation product with a mixed strain of lactobacillus and Candida utilis, wherein the mass ratio of the mixed strain to the primary degradation product is 1:150; the number of viable lactobacilli in the mixed strain is ≥10 9 cfu / g, viable count of Candida utilis ≥10 9cfu / g; sealed and fermented at 32°C for 2 days to obtain fermented material;

[0045] Step 4. Mix lactic acid, betaine and butanediol in a molar ratio of 6:6:80, heat and dissolve to obtain an ionic liquid; mix the fermentation material and the ionic liquid in a mass ratio of 1:5, perform 150MPa ultra-high pressure assisted extraction and intensive treatment for 2 minutes, let stand for 2 days, centrifuge at 3000rpm for 30 minutes, and take the supernatant to obtain the ionic liquid anti-allergic soothing composition.

[0046] Comparative Example 1

[0047] An ionic liquid anti-allergic soothing composition, the preparation process of which comprises the following steps:

[0048] Step 1: Take fresh whole herb of Selaginella; dry in the shade, and crush through a 40-mesh sieve to obtain plant powder;

[0049] Step 2: Mix plant powder, water and cellulase in a mass ratio of 1:15:0.05, wherein the activity of the cellulase is 200,000 U / g; adjust the pH to 4.5, heat to 48° C., perform enzymolysis for 4 hours under stirring, and inactivate the enzyme in a boiling water bath for 20 minutes to obtain a primary degradation product;

[0050] Step 3: Inoculate the primary degradation product with a mixed strain of lactobacillus and Candida utilis, wherein the mass ratio of the mixed strain to the primary degradation product is 1:150; the number of viable lactobacilli in the mixed strain is ≥10 9 cfu / g, viable count of Candida utilis ≥10 9 cfu / g; sealed and fermented at 32°C for 2 days to obtain fermented material;

[0051] Step 4. Mix lactic acid, betaine and butanediol in a molar ratio of 6:6:80, heat and dissolve to obtain an ionic liquid; mix the fermentation material and the ionic liquid in a mass ratio of 1:5, perform 150MPa ultra-high pressure assisted extraction and intensive treatment for 2 minutes, let stand for 2 days, centrifuge at 3000rpm for 30 minutes, and take the supernatant to obtain the ionic liquid anti-allergic soothing composition.

[0052] Comparative Example 2

[0053] An ionic liquid anti-allergic soothing composition, the preparation process of which comprises the following steps:

[0054] Step 1: Take fresh Rhodiola rosea root; dry it in the shade, and crush it through a 40-mesh sieve to obtain plant powder;

[0055] Step 2: Mix plant powder, water and cellulase in a mass ratio of 1:15:0.05, wherein the activity of the cellulase is 200,000 U / g; adjust the pH to 4.5, heat to 48° C., perform enzymolysis for 4 hours under stirring, and inactivate the enzyme in a boiling water bath for 20 minutes to obtain a primary degradation product;

[0056] Step 3: Inoculate the primary degradation product with a mixed strain of lactobacillus and Candida utilis, wherein the mass ratio of the mixed strain to the primary degradation product is 1:150; the number of viable lactobacilli in the mixed strain is ≥10 9 cfu / g, viable count of Candida utilis ≥10 9 cfu / g; sealed and fermented at 32°C for 2 days to obtain fermented material;

[0057] Step 4. Mix lactic acid, betaine and butanediol in a molar ratio of 6:6:80, heat and dissolve to obtain an ionic liquid; mix the fermentation material and the ionic liquid in a mass ratio of 1:5, perform 150MPa ultra-high pressure assisted extraction and intensive treatment for 2 minutes, let stand for 2 days, centrifuge at 3000rpm for 30 minutes, and take the supernatant to obtain the ionic liquid anti-allergic soothing composition.

[0058] Comparative Example 3

[0059] An ionic liquid anti-allergic soothing composition, the preparation process of which comprises the following steps:

[0060] Step 1: Take fresh Forsythia suspensa fruit; dry in the shade, crush through a 40-mesh sieve, and obtain plant powder;

[0061] Step 2: Mix plant powder, water and cellulase in a mass ratio of 1:15:0.05, wherein the activity of the cellulase is 200,000 U / g; adjust the pH to 4.5, heat to 48° C., perform enzymolysis for 4 hours under stirring, and inactivate the enzyme in a boiling water bath for 20 minutes to obtain a primary degradation product;

[0062] Step 3: Inoculate the primary degradation product with a mixed strain of lactobacillus and Candida utilis, wherein the mass ratio of the mixed strain to the primary degradation product is 1:150; the number of viable lactobacilli in the mixed strain is ≥10 9 cfu / g, viable count of Candida utilis ≥10 9 cfu / g; sealed and fermented at 32°C for 2 days to obtain fermented material;

[0063] Step 4. Mix lactic acid, betaine and butanediol in a molar ratio of 6:6:80, heat and dissolve to obtain an ionic liquid; mix the fermentation material and the ionic liquid in a mass ratio of 1:5, perform 150MPa ultra-high pressure assisted extraction and intensive treatment for 2 minutes, let stand for 2 days, centrifuge at 3000rpm for 30 minutes, and take the supernatant to obtain the ionic liquid anti-allergic soothing composition.

[0064] Comparative Example 4

[0065] An ionic liquid anti-allergic soothing composition, the preparation process of which comprises the following steps:

[0066] Step 1, taking fresh Selaginella whole herb, Rhodiola rosea root, and Forsythia suspensa fruit, wherein the mass ratio of the Selaginella whole herb, Rhodiola rosea root, and Forsythia suspensa fruit is 6:5:3; drying in the shade, and crushing through a 40-mesh sieve to obtain plant powder;

[0067] Step 2: Allow to stand for 2 days, centrifuge at 3000 rpm for 30 minutes, and take the supernatant to obtain the ionic liquid anti-allergic soothing composition.

[0068] Example 4

[0069] Mouse mononuclear macrophage leukemia cells (RAW264.7) are the most commonly used in vitro research model for screening anti-inflammatory active substances and studying inflammation. Under the action of inducers (such as lipopolysaccharide LPS), RAW264.7 cells will simulate inflammatory responses and release or upregulate a variety of inflammatory mediators such as nitric oxide (NO), cyclooxygenase-2 (COX-2), tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), etc.

[0070] The anti-inflammatory activity of the ionic liquid anti-allergic soothing composition prepared in Examples 1-3 and Comparative Examples 1-4 was determined by referring to the method described in the document "Chen Qingsheng, Meng Xiao, Liu Ting, et al. Research on the soothing and repairing effect of an anti-allergic repair composition on sensitive skin [J]. Surfactant Chemical Industry, 2021, 51(12): 1221-1227."

[0071] The cells in the logarithmic growth phase and in good condition were digested, centrifuged, suspended in the corresponding cell culture medium, and the cells were counted to prepare a cell suspension with a certain cell concentration. The experiment was designed as a blank group (PBS group), a control group (LPS group) and a sample group, with 3 parallel wells in each group. 200 μL of cell suspension was inoculated into each well of a 48-well plate and incubated at 37°C and 5% CO 2Under the conditions, culture overnight for (14±2)h, discard the culture medium, and wash the cells with 200μL / well saline or PBS. In the blank group, 195μL culture medium and 5μL PBS were added to each well, in the control group, 191L ​​culture medium, 4μL LPS working solution (LPS final concentration was 1μg / mL) and 5μL PBS were added to each well, and in the sample group, 191μL culture medium, 4μL LPS working solution (LPS final concentration was 1μg / mL) and 5μL sample to be tested were added to each well. 37℃, 5% CO 2 The cells were cultured for 24 h under the same conditions, the cell morphology was observed, the cell supernatant was collected, and the IL-6 and TNF-α contents were detected and calculated according to the instructions of the kit. The specific test results are shown in Table 1.

[0072] Table 1 Effects of each test group on the release of IL-6 and TNF-α in RAW264.7 cells

[0073] Test Group IL-6 / (pg / mL) TNF-α / (pg / mL) Example 1 1280 4250 Example 2 1320 4410 Example 3 1150 4060 Comparative Example 1 1450 8350 Comparative Example 2 1530 7800 Comparative Example 3 1280 7450 Comparative Example 4 1290 6540 Blank Group 50 3600 Control group 1650 12800

[0074] As can be seen from Table 1, compared with the blank group, the TNF-α secretion level of the control group was significantly increased, indicating that the stimulation conditions of this test were effective. Compared with the control group, the ionic liquid anti-allergic soothing compositions prepared in Examples 1-3 of the present invention can significantly reduce the content of TNF-α, and the effect is better than that of Comparative Examples 1-3, which only add one of the raw materials of Selaginella whole herb, Rhodiola rosea root, and Forsythia suspensa fruit, and Comparative Example 4 using the decoction method.

[0075] As can be seen from Table 1, compared with the blank group, the IL-1α secretion level of the control group was significantly increased, indicating that the stimulation conditions of this test were effective. Compared with the control group, the ionic liquid anti-allergic soothing compositions prepared in Examples 1-3 of the present invention can significantly reduce the content of IL-1α, and the effect is better than that of Comparative Examples 1-3, which only add one of the raw materials of Selaginella herb, Rhodiola rosea root, and Forsythia suspensa fruit, and Comparative Example 4 using the decoction method.

[0076] Example 5

[0077] An anti-allergic soothing facial mask is composed of the following raw materials in percentage by weight: 1% of squalane, 5% of 1,3-propylene glycol, 3% of glycerol, 0.2% of polyglycerol-10 diisostearate, 0.1% of lecithin, 0.5% of p-hydroxyacetophenone, 0.5% of 1,2-hexanediol, 0.01% of ceramide NP, 0.2% of carbomer, 0.05% of hydroxyethyl cellulose, 0.05% of sodium hyaluronate, 0.2% of arginine, 5% of the ionic liquid anti-allergic soothing composition prepared in Example 3, and the balance of water.

[0078] Preparation method: put squalane, polyglyceryl-10 diisostearate and ceramide into a stirring pot according to a proportion and heat to 80°C as the oil phase; put 1,3-propylene glycol, water, glycerin, lecithin, p-hydroxyacetophenone and 1,2-hexanediol into an emulsifying pot, start homogenization, add carbomer, hydroxyethyl cellulose and sodium hyaluronate, stir and heat to 80°C to prepare the water phase; then add the oil phase to the water phase, homogenize at a rate of 1000 rpm for 3 minutes, and stir to cool down; when the sample temperature drops to 65°C, add arginine to the water phase, stir evenly, and homogenize for 2 minutes; finally, when the temperature drops to 45°C, add the ionic liquid anti-allergic soothing composition to the water phase, stir evenly.

[0079] Example 6

[0080] The lactate stinging test (LST) is a test method designed to evaluate sensitive skin. This method simulates skin irritation by applying lactic acid solutions of different concentrations on the skin and observes the reaction of the tested skin to evaluate the sensitivity. This method is widely used in medicine, cosmetics and other fields.

[0081] The specific method is: select the nasolabial fold area of ​​the subject for the test, drop 30 μL of 10% lactic acid solution on a single-layer filter paper with a diameter of 0.8 cm, ask the subjects about the tingling sensation at 3 minutes, 4 minutes and 5 minutes respectively, and score according to the 4-point method (0 point for no tingling sensation, 1 point for mild tingling, 2 points for moderate tingling, and 3 points for severe tingling). Those with a cumulative tingling sensation of ≥ 3 points are considered to have lactic acid tingling.

[0082] 30 volunteers were organized to undergo a lactic acid stinging test, and the data on day 0 were measured. Then, the anti-allergic soothing facial mask prepared in Example 5 was used once in the morning and evening for one month, and the lactic acid stinging test was performed on the 14th and 28th days. The specific test results are shown in Table 2.

[0083] Table 2 Human lactic acid sting test data

[0084] project D0 day D14 day D28 days Mean lactic acid sting score 3.1±1.2 2.9±0.9 2.6±1.1

[0085] As can be seen from Table 1, after continuously using the anti-allergic soothing facial mask prepared by the present invention for one month, the average lactic acid stinging score of the volunteers decreased from 3.1 to 2.6, indicating that the anti-allergic soothing facial mask prepared by the present invention can effectively maintain the skin barrier, reduce skin sensitivity, and achieve good anti-allergic and soothing effects.

[0086] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. An ionic liquid anti-allergic soothing composition, characterized in that: Contains Selaginella tamariscina extract, Rhodiola rosea root extract, and Forsythia suspensa fruit extract.

2. The ionic liquid anti-allergic soothing composition according to claim 1, characterized in that: The preparation process includes the following steps: Step 1: Take fresh Selaginella whole herb, Rhodiola rosea root, and Forsythia suspensa fruit, dry them in the shade, crush and sieve them to obtain plant powder; Step 2: Mix plant powder, water and cellulase, perform enzymolysis under heating and stirring, and inactivate the enzyme at high temperature to obtain primary degradation products; Step 3: inoculating a mixed strain of lactobacillus and Candida utilis into the primary degradation product, fermenting and culturing, and obtaining a fermentation material; Step 4: mix lactobionic acid, betaine and butanediol in a molar ratio of 1-10:1-10:50-100, heat and dissolve to obtain an ionic liquid; mix the fermentation material and the ionic liquid in a mass ratio of 1:1-10, perform ultrahigh pressure assisted extraction and enhanced treatment, let stand for aging, centrifuge and obtain the supernatant to obtain the ionic liquid anti-allergic soothing composition.

3. The ionic liquid anti-allergic soothing composition according to claim 2, characterized in that: The mass ratio of the whole herb of Selaginella, the root of Rhodiola rosea and the fruit of Forsythia suspensa is 1-10:1-10:1-10.

4. The ionic liquid anti-allergic soothing composition according to claim 2, characterized in that: The sieving is through a 30-50 mesh sieve.

5. The ionic liquid anti-allergic soothing composition according to claim 2, characterized in that: The activity of the cellulase is 100,000-200,000 U / g; the mass ratio of the plant powder, water and cellulase is 1:10-20:0.01-0.

1.

6. The ionic liquid anti-allergic soothing composition according to claim 2, characterized in that: The enzymatic hydrolysis conditions are as follows: pH 4.0-5.0, temperature 45-50° C., hydrolysis for 3-5 hours, and then boiling water bath for 20 minutes to inactivate the enzyme.

7. The ionic liquid anti-allergic soothing composition according to claim 2, characterized in that: The mass ratio of the mixed strain to the primary degradation product is 1:100-200; the number of viable lactobacilli in the mixed strain is ≥10 9 cfu / g, viable count of Candida utilis ≥10 9 cfu / g.

8. The ionic liquid anti-allergic soothing composition according to claim 2, characterized in that: The fermentation culture condition is sealed fermentation at 30-35°C for 1-3 days.

9. The ionic liquid anti-allergic soothing composition according to claim 2, characterized in that: The ultra-high pressure assisted extraction condition is 100-200 MPa ultra-high pressure assisted extraction for 1-3 minutes.

10. Use of the ionic liquid anti-allergic soothing composition according to any one of claims 1 to 9 in the preparation of cosmetics.