Composition for improving anti-aging and tightening effects and preparation method thereof

Abstract

The invention discloses a composition for improving anti-aging and tightening effects and a preparation method thereof, and belongs to the technical field of cosmetics, the composition for improving anti-aging and tightening effects comprises the following raw materials by mass: 20-60 parts of galactose yeast-like bacterium fermentation product filtrate; 0.1 to 5 parts of nicotinamide adenine dinucleotide; 0.5 to 5 parts of myristyl nicotinate; 0.2 to 4.5 parts of a cynara scolymus leaf extract; 0.1 to 5.5 parts of a lens culinaris seed extract; and 0.3-5 parts of a chrysanthellum indicum extract. The composition for improving the anti-aging and tightening effects, provided by the invention, has remarkable tightening and anti-aging effects, and is integrally mild and non-irritant.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to a composition for improving anti-aging and firming effects and a preparation method thereof. Background Art

[0002] With the change of modern lifestyle and the increase of environmental pressure, skin health issues are receiving more and more attention. Skin aging is a natural physiological process, but factors such as ultraviolet exposure, environmental pollution, and stress accelerate this process, leading to the appearance of skin sagging, wrinkles, spots and other problems. In addition, modern consumers have higher and higher requirements for the efficacy and safety of skin care products, and expect to achieve efficient anti-aging and firming effects through scientific formulas.

[0003] In this context, it is particularly important to develop a skin care product with comprehensive anti-aging and firming effects. Summary of the invention

[0004] The object of the present invention is to provide a composition for improving anti-aging and firming effects and a preparation method thereof.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] In a first aspect, the present invention provides a composition for improving anti-aging and firming effects, the composition comprising the following raw materials in parts by weight:

[0007] Galactomyces yeast fermentation product filtrate: 20-60 parts;

[0008] Nicotinamide adenine dinucleotide: 0.1-5 parts;

[0009] Myristyl nicotinate: 0.5-5 parts;

[0010] Artichoke leaf extract: 0.2-4.5 parts;

[0011] Lentil extract: 0.1-5.5 parts;

[0012] Golden chamomile extract: 0.3-5 parts.

[0013] This study found that the combined use of the above six ingredients can synergistically enhance the effects and achieve excellent firming and anti-aging effects.

[0014] As a preferred embodiment of the composition for improving the anti-aging and firming effect of the present invention, the composition for improving the anti-aging and firming effect comprises the following raw materials in parts by weight:

[0015] Galacto-saccharomyces cerevisiae fermentation product filtrate: 40-60 parts;

[0016] Nicotinamide adenine dinucleotide: 0.1-4 parts;

[0017] Myristyl Nicotinate: 0.5-4 parts;

[0018] Artichoke leaf extract: 2-4.5 parts;

[0019] Lentil extract: 3-5.5 parts;

[0020] Golden chamomile extract: 2-5 parts.

[0021] As the most preferred embodiment of the composition for improving the anti-aging and firming effect of the present invention, the composition for improving the anti-aging and firming effect comprises the following raw materials in parts by weight:

[0022] Galactomyces galactosidase fermentation product filtrate: 60 parts;

[0023] Nicotinamide adenine dinucleotide: 4 parts;

[0024] Myristyl Nicotinate: 4 parts;

[0025] Artichoke leaf extract: 2 parts;

[0026] Lentil extract: 3 parts;

[0027] Golden chamomile extract: 2 parts.

[0028] The present invention has found that the mass fraction of each raw material in a composition for improving anti-aging and firming effects will affect the performance of the product. When the mass fraction of the raw materials is further selected to be within the above range (including the endpoint value), the obtained product can more significantly improve skin firmness and anti-aging effects.

[0029] In a second aspect, the present invention provides an essence for improving anti-aging and firming effects, wherein the essence contains the composition for improving anti-aging and firming effects described in the first aspect.

[0030] As an embodiment of the essence of the present invention, the essence comprises the following components in percentage by mass:

[0031] Compositions that enhance anti-aging and firming effects: 1%-3%;

[0032] Dipropylene glycol: 50%-80%;

[0033] PEG-40 hydrogenated castor oil: 0.5%-3%;

[0034] Make up to 100% with ultrapure water.

[0035] In a third aspect of the present invention, the present invention provides a method for preparing the essence for enhancing the anti-aging and firming effect according to the second aspect, wherein the preparation method specifically comprises the following steps:

[0036] S1: Mix 1 / 2 ultrapure water and dipropylene glycol evenly to obtain a mixed solution A;

[0037] S2: Evenly mix the composition for enhancing anti-aging and firming effect, PEG-40 hydrogenated castor oil, and the remaining ultrapure water to obtain a mixed solution B;

[0038] S3: Mix the mixed solution A and the mixed solution B, and homogenize for 4-6 hours to obtain an essence with enhanced anti-aging and firming effects.

[0039] Compared with the prior art, the present invention has the following beneficial effects:

[0040] The present invention provides a composition for enhancing anti-aging and firming effects and a preparation method thereof. The six components in the composition cooperate with each other and synergize to achieve the effect of enhancing skin anti-aging and firming, and the overall effect is mild and non-irritating. Specifically, the filtrate of the fermentation product of galactosyl yeast has significant antioxidant and anti-inflammatory activity. It can inhibit the oxidative stress of proinflammatory cytokines by activating the antioxidant system, thereby reducing the damage of free radicals to skin cells and delaying skin aging. The various bioactive ingredients in the fermentation product can promote skin cell metabolism and regeneration, and enhance the self-repair ability of the skin; nicotinamide adenine dinucleotide is a key factor in cell redox, which can participate in cell energy metabolism and DNA repair. In the anti-aging process, it can maintain the normal function of cells and slow down cell aging caused by insufficient energy metabolism. In addition, nicotinamide adenine dinucleotide can activate the Sirtuins family protein signaling pathway, thereby slowing down cell aging; myristyl nicotinate is decomposed into nicotinic acid in the skin, which can significantly stimulate cell regeneration and The stratum corneum is renewed, thereby reducing the formation of wrinkles and achieving anti-aging effects; cardoon leaf extract is rich in active substances, some of which can effectively inhibit the activation of NF-kB, thereby reducing inflammatory responses, and most active substances such as polyphenols and ketones have strong antioxidant properties, which can effectively scavenge free radicals and reduce skin damage caused by oxidative stress. In addition, polyphenol active substances can also promote skin collagen synthesis and enhance skin elasticity and firmness; lentil seed extract can significantly promote fibroblast activity, thereby improving skin firmness and elasticity; golden chamomile extract contains a variety of triterpenoid compounds, which have significant anti-inflammatory effects and can relieve skin sensitivity and inflammation. In addition, the flavonoids contained in golden chamomile extract have a certain inhibitory effect on melanocytes.

[0041] Therefore, nicotinamide adenine dinucleotide can delay aging through energy metabolism and DNA repair; galactosaccharomyces fermentation product filtrate, artichoke leaf extract and golden chamomile extract can reduce cell damage through antioxidant and anti-inflammatory effects. The four work together to protect skin cells more comprehensively; lentil seed extract and artichoke leaf extract can promote collagen regeneration, while myristyl nicotinate indirectly enhances collagen renewal by promoting cell regeneration. The three work together to significantly improve skin firmness. The above six raw materials play a role in multiple aspects such as antioxidant, anti-inflammatory, promoting cell regeneration, and enhancing collagen synthesis. Through multi-target intervention, the skin's anti-aging ability is improved and the firming effect is enhanced. DETAILED DESCRIPTION

[0042] In order to better understand the present invention, the present invention is further described below in conjunction with specific examples, wherein the terms used in "composition" and "example" are intended to describe specific embodiments and do not constitute limitations on the scope of protection of the present invention.

[0043] The experimental methods in the following examples without specifying specific conditions are usually carried out under conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, the percentages and mass fractions are calculated.

[0044] Some raw materials and their sources are as follows:

[0045] Galacto-saccharomyces cerevisiae fermentation product filtrate: purchased from Guangzhou Mingtu Biotechnology Co., Ltd.

[0046] Nicotinamide adenine dinucleotide: purchased from Zhejiang Invodi Biotechnology Co., Ltd.

[0047] Myristyl nicotinate: purchased from Shanghai Keqin Technology Co., Ltd.

[0048] Cardoon leaf extract: purchased from Zhenghe (Guangzhou) Biotechnology Co., Ltd., product number: L2-SCB2730.

[0049] Lentil seed extract: purchased from Shaanxi Milcon Biotechnology Co., Ltd., product number: 2020060502.

[0050] Golden chamomile extract: purchased from Xi'an Qiuhe Biotechnology Co., Ltd., product number: QH2024151-1.

[0051] Dipropylene glycol: purchased from Dow Chemical Company.

[0052] PEG-40 hydrogenated castor oil: purchased from BASF SE.

[0053] The water used in the present invention is ultrapure water unless otherwise specified; other raw materials, test reagents and cells are commercially available.

[0054] The weight proportions of the raw materials in a composition for improving anti-aging and firming effects are shown in Table 1.

[0055] Table 1 Ratio of each raw material by weight in a composition for improving anti-aging and firming effect

[0056]

[0057] The components and mass percentages of an essence for enhancing anti-aging and firming effects are shown in Table 2.

[0058] Table 2 Components and mass percentages of an essence for improving anti-aging and firming effects

[0059]

[0060] The preparation methods of Examples 1-5 are specifically as follows:

[0061] S1: Mix 1 / 2 ultrapure water and dipropylene glycol evenly to obtain a mixed solution A;

[0062] S2: Evenly mix the composition for enhancing anti-aging and firming effect, PEG-40 hydrogenated castor oil, and the remaining ultrapure water to obtain a mixed solution B;

[0063] S3: Mix the mixed solution A and the mixed solution B, and homogenize for 5 hours to obtain an essence with enhanced anti-aging and firming effects.

[0064] Experiment 1: Firming and anti-wrinkle efficacy test

[0065] Cells: Human keratinocytes (HKC).

[0066] Test substances: Compositions 1-3, compositions ①-⑥.

[0067] Concentration of test substance: Prepare a 20% (m / v) solution of the test substance using 60 v / v% ethanol aqueous solution, then dilute it to 2% using cell culture medium at a volume ratio of 1:10, and use it immediately after preparation.

[0068] Positive sample: Vitamin E.

[0069] Positive sample concentration: Use 60v / v% ethanol aqueous solution to dilute vitamin E to a 5% (m / v) solution, and then dilute it to 0.05% with cell culture medium at a volume ratio of 1:100, and prepare it before use.

[0070] Experimental method: Refer to T / SHRH 032-2020 "Cosmetic firming and anti-wrinkle efficacy test-in vitro keratinocyte reactive oxygen species (ROS) inhibition test method".

[0071] The specific results are shown in Table 3, where X is the mean and SD is the standard deviation.

[0072] Table 3 Firming and anti-wrinkle efficacy test results

[0073]

[0074]

[0075] Note: "#" indicates that compared with composition 1, p < 0.05, which is significant.

[0076] According to the results in Table 3, compositions 1-3 have significant firming and anti-wrinkle effects; by comparing composition 1 with compositions ①-⑤, it can be seen that there is a significant synergistic effect between the filtrate of the fermentation product of galactosaccharomyces cerevisiae and nicotinamide adenine dinucleotide, myristyl nicotinate, artichoke leaf extract, lentil seed extract, and golden chamomile extract in the present invention. By comparing composition 1 with composition ⑥, it can be seen that the filtrate of the fermentation product of galactosaccharomyces cerevisiae, nicotinamide adenine dinucleotide, myristyl nicotinate, artichoke leaf extract, lentil seed extract, and golden chamomile extract in the present invention have a synergistic effect only within a specific dosage ratio range.

[0077] Test 2: Antioxidant test

[0078] Preparation of DPPH solution: Dissolve 0.002 g DPPH in 50 mL 60 v / v% ethanol aqueous solution to make a 0.1 mM DPPH solution and store in dark.

[0079] Sample preparation: Use 60 v / v% ethanol aqueous solution to prepare compositions 1-3 and compositions ①-⑥ into 2 wt% sample solutions, and store in the dark.

[0080] Positive group preparation: Vc was prepared into 0.1wt% Vc solution using 60v / v% ethanol aqueous solution and stored in the dark.

[0081] Experimental method: Sample groups, positive groups, blank groups, and control groups were added to 96-well plates; among them:

[0082] Sample group: 100 μL sample solution + 100 μL DPPH solution (3 wells per concentration);

[0083] Blank group: 100 μL sample solution + 100 μL 60 v / v% ethanol aqueous solution (3 wells per concentration);

[0084] Positive group: 100 μL of Vc solution + 100 μL of 60 v / v% ethanol aqueous solution (3 wells for each concentration);

[0085] Control group: 100 μL of DPPH solution + 100 μL of 60 v / v% ethanol aqueous solution.

[0086] After preparing each group of test samples, incubate them in the dark at room temperature for 30 minutes, and then measure the absorbance at 517 nm, take the average, and calculate the DPPH clearance rate of samples of each concentration (X±SD) by the following formula, where X is the mean and SD is the standard deviation.

[0087] Clearance rate = (1-(A sample-A blank) / A control)*100%;

[0088] Wherein, A sample is the absorbance value of the sample group or the positive group; A blank is the absorbance value of the blank group; A control is the absorbance value of the control group.

[0089] See Table 4 for the results.

[0090] Table 4 Antioxidant test results

[0091] Experimental Group Clearance rate / %(X±SD) Composition 1 89.54±1.90％ Composition 2 86.76±4.14％ Composition 3 89.14±3.89％ Composition ① 68.29±7.45％# Composition ② 63.97±5.36％# Composition ③ 61.11±2.16％# Composition ④ 65.77±9.97％# Composition⑤ 60.02±5.95％# Composition ⑥ 73.06±1.16％# Positive samples 100％

[0092] "#" indicates that compared with composition 1, p < 0.05, which is significant.

[0093] According to the results in Table 4, compositions 1-3 have significant firming and anti-wrinkle effects; by comparing composition 1 with compositions ①-⑤, it can be seen that there is a significant synergistic effect between the filtrate of the fermentation product of galactosyl yeast-like bacteria and nicotinamide adenine dinucleotide, myristyl nicotinate, artichoke leaf extract, lentil seed extract, and golden chamomile extract in the present invention. By comparing composition 1 with composition ⑥, it can be seen that the filtrate of the fermentation product of galactosyl yeast-like bacteria, nicotinamide adenine dinucleotide, myristyl nicotinate, artichoke leaf extract, lentil seed extract, and golden chamomile extract in the present invention have a synergistic effect only within a specific dosage ratio range.

[0094] Test 3: Safety test

[0095] The present invention experiments verify the biosafety of Examples 1-5.

[0096] The 2015 "Technical Specifications for Safety of Cosmetics" was used as a reference standard for the irritation evaluation of cosmetics. The test method was a skin patch test, and 50 people aged 16-65 years were randomly distributed and tested.

[0097] Test method: Put the test substance into the patch tester, the dosage is 0.020-0.025g, cover the patch tester with the test substance on the back or forearm of the subject with non-irritating cloth-based tape, and gently press it with the palm to make it evenly adhere to the skin surface for 24 hours. After removing the test patch tester, observe the skin reaction after the indentation disappears 30 minutes later. If the result is negative, observe again 24 hours and 48 hours after the patch test.

[0098] Evaluation criteria:

[0099] Grade 0: negative reaction;

[0100] Grade 1: Suspicious reaction, only slight erythema;

[0101] Grade 2: weak positive reaction, erythema, infiltration, edema, and papules may be present;

[0102] Grade 3: Strong positive reaction, erythema, infiltration, edema, papules, and the reaction may extend beyond the test area;

[0103] Grade 4: Very strong positive reaction, obvious erythema, severe infiltration, edema, confluent herpes, and the reaction exceeds the test area.

[0104] Test results: Skin reactions were negative in all subjects.

[0105] The embodiments described above are part of the embodiments of the present invention, rather than all of the embodiments. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention.

Claims

1. A composition for improving anti-aging and firming effects, characterized in that: Including the following raw materials by weight: Galactomyces yeast fermentation product filtrate: 20-60 parts; Nicotinamide adenine dinucleotide: 0.1-5 parts; Myristyl nicotinate: 0.5-5 parts; Artichoke leaf extract: 0.2-4.5 parts; Lentil extract: 0.1-5.5 parts; Golden chamomile extract: 0.3-5 parts.

2. The composition according to claim 1, characterized in that Including the following raw materials by weight: Galacto-saccharomyces cerevisiae fermentation product filtrate: 40-60 parts; Nicotinamide adenine dinucleotide: 0.1-4 parts; Myristyl Nicotinate: 0.5-4 parts; Artichoke leaf extract: 2-4.5 parts; Lentil extract: 3-5.5 parts; Golden chamomile extract: 2-5 parts.

3. The composition according to claim 1, characterized in that Including the following raw materials by weight: Galactomyces galactosidase fermentation product filtrate: 60 parts; Nicotinamide adenine dinucleotide: 4 parts; Myristyl Nicotinate: 4 parts; Artichoke leaf extract: 2 parts; Lentil extract: 3 parts; Golden chamomile extract: 2 parts.

4. An essence for enhancing anti-aging and firming effects, characterized in that: A composition for enhancing the anti-aging and firming effect according to any one of claims 1 to 3.

5. The essence according to claim 4, characterized in that: Contains the following components by mass percentage: Compositions that enhance anti-aging and firming effects: 1%-3%; Dipropylene glycol: 50%-80%; PEG-40 hydrogenated castor oil: 0.5%-3%; Make up to 100% with ultrapure water.

6. A method for preparing the essence according to claim 5, characterized in that: The following steps are involved: S1: Mix 1 / 2 ultrapure water and dipropylene glycol evenly to obtain a mixed solution A; S2: Evenly mix the composition for enhancing anti-aging and firming effect, PEG-40 hydrogenated castor oil, and the remaining ultrapure water to obtain a mixed solution B; S3: Mix the mixed solution A and the mixed solution B, and homogenize for 4-6 hours to obtain an essence with enhanced anti-aging and firming effects.

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