Bacteriostatic hand sanitizer containing plant extract and preparation method of bacteriostatic hand sanitizer

Abstract

The invention relates to a natural bacteriostatic hand sanitizer, in particular to a bacteriostatic formula combining three plant extracts of camellia, radix angelicae and rosemary. The hand sanitizer provides a broad-spectrum antibacterial effect through the synergistic effect of natural plant extracts, and is suitable for daily and medical cleaning requirements. The formula comprises a camellia extract, an angelica dahurica extract, a rosemary extract and components with moisturizing and moistening effects. The hand sanitizer disclosed by the invention has the advantages of being natural, mild, non-irritating to skin and lasting in antibacterial effect, does not contain a traditional chemical antibacterial agent, and meets the requirements of environmental protection and health. Therefore, the hand sanitizer disclosed by the invention has a good application prospect.

Description

Technical Field

[0001] The invention relates to the field of hand sanitizer, in particular to an antibacterial hand sanitizer containing plant extracts and a preparation method thereof. Background Art

[0002] As a daily personal hygiene product, hand sanitizer plays an important role in effectively reducing the spread of pathogenic microorganisms. However, most common hand sanitizers on the market contain chemically synthesized antibacterial ingredients, such as triclosan and benzalkonium chloride. These chemical ingredients can effectively inhibit the growth of pathogenic microorganisms such as bacteria and fungi in the short term, but they may also bring adverse effects. First, excessive use of chemical antibacterial agents may cause bacteria to develop drug resistance, especially in public places such as hospitals and schools. The drug resistance problem will lead to a decrease in the sensitivity of pathogens to traditional antibacterial agents, weakening the effectiveness of antibacterial products. Secondly, some chemical ingredients, such as triclosan, have been confirmed by studies to have potential health risks to the skin and endocrine system. Long-term use may cause side effects such as skin allergies and endocrine disorders. At the same time, some chemical antibacterial agents degrade slowly in water environments, which may have a negative impact on the ecosystem and become a source of environmental pollution.

[0003] In recent years, as people's awareness of health and environmental protection increases, the market demand for natural, safe, and low-irritation hand sanitizers has gradually increased. Plant extracts are widely used in the research and development of antibacterial skin care products due to their natural active ingredients and mild and non-irritating properties. For example, honeysuckle extract shows a broad-spectrum antibacterial effect with its rich chlorogenic acid and flavonoid components, and has a significant inhibitory effect on common Staphylococcus aureus and Escherichia coli; aloe vera extract contains moisturizing ingredients such as polysaccharides and amino acids, which can protect the skin barrier while inhibiting bacteria and has a good repair effect; tea tree essential oil has excellent antibacterial and antioxidant properties due to its terpenoid components, and is widely used in the antibacterial field. Compared with chemical antibacterial agents, these plant extracts are not easy to develop drug resistance, are mild and non-irritating to the skin, and are suitable for frequent use.

[0004] However, natural plant extracts are gradually being used in antibacterial products due to their mild and safe properties. However, the antibacterial effect of a single plant extract is limited, and formula optimization to achieve natural broad-spectrum antibacterial effects and protect the skin barrier has become the focus of current research. Summary of the invention

[0005] The present invention aims to provide a natural antibacterial hand soap based on plant extracts, which significantly enhances the antibacterial effect on a variety of pathogenic microorganisms through the synergistic effect of camellia, angelica and rosemary extracts, and provides a safe, environmentally friendly, mild and non-irritating cleaning solution for the skin.

[0006] Therefore, on one hand, the present invention discloses a natural antibacterial hand sanitizer containing plant extracts, which comprises the following ingredients: 0.5-1% of camellia extract, 0.5-1% of angelica dahurica extract, 0.5-1% of rosemary extract, 1% of vitamin E, 5% of vegetable glycerin, 10% of coconut glucoside, 0.5% of xanthan gum and the balance of deionized water, wherein the mass ratio of the camellia extract, the angelica dahurica extract and the rosemary extract is 1:1:1.

[0007] Preferably, the hand sanitizer of the present invention comprises the following ingredients: 0.5% camellia extract, 0.5% angelica extract, 0.5% rosemary extract, 1% vitamin E, 5% vegetable glycerin, 10% coconut glucoside, 0.5% xanthan gum and the balance deionized water.

[0008] In another aspect, the present invention also discloses a method for preparing the antibacterial hand sanitizer, the method comprising the following steps:

[0009] Step 1: Extracting plant active ingredients, specifically including the following steps:

[0010] (1) Camellia extract: dried camellia petals were crushed and pretreated at -20°C for 24 hours; 10 g of camellia powder was added to 150 ml of 25% ethanol solution at a solid-liquid ratio of 1:15; ultrasonic-assisted and microwave-assisted extraction was performed, with the specific conditions being an ultrasonic power of 300 W, a frequency of 40 kHz, a microwave power of 200 W, a temperature controlled at 30°C, and an extraction time of 20 minutes; after filtering the extract, dynamic countercurrent extraction and purification were performed, and then low-temperature, reduced-pressure drying was performed at 40°C to obtain camellia extract powder;

[0011] (2) Angelica dahurica extract: 10 g of dried Angelica dahurica powder was mixed with 100 ml of 70% ethanol solution at a ratio of 1:10, and the mixture was extracted in a continuous stirring extractor for 30 minutes; the extract was filtered and concentrated to a viscous state, and the concentrate was placed in a supercritical CO2 extraction device at a pressure of 30 MPa and a temperature of 40°C for 20 minutes; the extract was freeze-dried to obtain Angelica dahurica extract powder;

[0012] (3) Rosemary extract: 10 g of rosemary dry powder was added to 100 ml of deionized water at a ratio of 1:10, and 0.1% sodium citrate was added as an auxiliary agent; in a water bath at 45° C., ultrasonic waves were used with a power of 300 W for resonance extraction for 1 hour; the extract was filtered and impurities were removed by molecular distillation; and the extract was freeze-dried to obtain rosemary extract powder;

[0013] Step 2: Prepare hand sanitizer.

[0014] Preferably, step 2 of the present invention comprises the following steps:

[0015] (1) Add 75% deionized water into a mixing container, add camellia, angelica dahurica, and rosemary extracts in sequence, and stir until uniformly dissolved;

[0016] (2) Add 5% glycerol and 1% vitamin E and continue stirring for 5 minutes;

[0017] (3) slowly adding 10% coco-glucoside as a mild surfactant, and controlling the stirring speed at 300 rpm to avoid foaming;

[0018] (4) Add 0.5% xanthan gum as a thickener to adjust the viscosity to an appropriate level;

[0019] (5) Adjust the pH value of hand sanitizer to 5.8-6.2 to ensure mildness;

[0020] (6) Place the product in a sterilized container and seal it for storage.

[0021] In another aspect, the present invention also discloses an application of the method in preparing a natural antibacterial hand sanitizer containing plant extracts.

[0022] The hand sanitizer of the present invention uses natural plant extracts instead of chemical antibacterial agents, does not contain irritating ingredients, and is suitable for various skin types, especially sensitive skin. The combination of camellia, angelica dahurica and rosemary extracts shows a good antibacterial effect on a variety of bacteria (such as Staphylococcus aureus, Escherichia coli, etc.). Vegetable glycerin and vitamin E are specially added to the formula to effectively reduce skin dryness after washing hands and maintain skin moisture and elasticity. The ingredients are all derived from natural plants, meet environmental protection requirements, do not pollute water resources, and are suitable for modern environmental protection concepts.

[0023] The present invention combines camellia, angelica and rosemary extracts. The synergistic effect of the three not only enhances the antibacterial effect, but also supports each other in anti-oxidation, skin protection and other aspects, significantly improving the multifunctionality of the hand sanitizer. At the same time, it also overcomes the antagonistic effect of the three. Compared with single or two-by-two plant extracts, the combination of the three makes the hand sanitizer have a broader spectrum and more comprehensive effect, providing a better user experience, especially in terms of protection, repair and moisturizing. The specific possible synergistic principle is as follows:

[0024] (1) Enhanced antibacterial effect: Camellia is rich in tea polyphenols and flavonoids, which have strong antioxidant and antibacterial activity, especially against Gram-positive bacteria (such as Staphylococcus aureus). Tea polyphenols can destroy the bacterial cell membrane structure and inhibit bacterial metabolism to achieve a bactericidal effect. The coumarin compounds and volatile oils contained in Angelica dahurica have strong antibacterial effects and can effectively inhibit a variety of pathogens (including Escherichia coli, Candida albicans, etc.). Its volatile oil components can penetrate the bacterial cell wall, destroy the internal structure of the cell, and prevent bacterial growth. Rosmarinic acid and essential oil components (such as rosmarinic alcohol) in rosemary have significant antibacterial, antiviral, and antifungal effects. Its antibacterial mechanism includes reducing bacterial proliferation by inhibiting the respiratory chain function of bacteria and destroying the structure of the cell wall. These three plant extracts each inhibit bacteria and other pathogenic microorganisms through different mechanisms, complementing each other to form a more broad-spectrum antibacterial system. The active ingredients of camellia, angelica and rosemary extracts work synergistically, significantly improving the inhibitory effect on different types of pathogenic microorganisms and enhancing the overall antibacterial ability.

[0025] (2) Comprehensive skin protection effect: Camellia extract is rich in oleic acid and flavonoids, which can effectively moisturize the skin and promote skin repair. Its antioxidant effect also helps to slow down skin aging and protect the skin from environmental stress. The aromatic compounds and volatile oil components contained in Angelica dahurica extract have a calming and anti-inflammatory effect on the skin, and can relieve skin irritation and allergic reactions caused by frequent hand washing or the use of chemical substances. In addition, Angelica dahurica also has a positive effect on promoting skin microcirculation and metabolism. The rosmarinic acid and essential oil components contained in rosemary extract not only have antibacterial effects, but also promote blood circulation in the skin, enhance the repair ability of skin cells, and reduce skin inflammation and damage. The three have a complementary effect on skin protection. The moisturizing effect provided by camellia and the anti-inflammatory and calming effect of Angelica dahurica complement each other, and the blood circulation and cell repair effects of rosemary enhance the recovery ability of the skin barrier. This combination can maintain a good cleaning effect while avoiding the discomfort symptoms such as dryness, redness and swelling caused by frequent use of hand sanitizer.

[0026] (3) Enhanced antioxidant and anti-aging effects: Camellia extract is rich in catechins and vitamin E, both of which have strong antioxidant effects and can remove free radicals on the skin surface and in cells, delaying the skin aging process. Angelica dahurica extract is rich in coumarins and flavonoids, which also have significant antioxidant effects. They can protect the skin from damage by external environmental factors such as ultraviolet rays and pollution, and promote the self-repair of skin cells. Rosmarinic acid and piperic acid in rosemary have strong antioxidant effects and can effectively fight against oxidative damage caused by the environment and slow down skin aging. These three plant extracts work synergistically through their different antioxidant mechanisms to comprehensively fight against free radical damage and enhance the skin's antioxidant capacity. Compared with single plant extracts, the combination of the three can provide stronger anti-aging effects and enhance the skin's self-repair and anti-aging functions.

[0027] (4) Reduce the limitations of a single ingredient: Although camellia, angelica dahurica or rosemary can provide certain antibacterial and anti-inflammatory effects when used alone, the antibacterial spectrum of a single plant is usually limited; for example, camellia may not be effective against certain bacteria, and the antibacterial effects of angelica dahurica and rosemary are also affected by their extraction concentration and quality. Combining plant extracts in pairs can produce certain synergistic effects, but there may still be problems with the antibacterial spectrum being not broad enough or the skin protection effect being not comprehensive enough. The three plant extracts are complementary in terms of antibacterial, antioxidant and skin protection, and the synergistic effect greatly improves the antibacterial range and skin repair effect. The combination of the three can provide a wider range of protection while achieving strong antibacterial effects, reducing the shortcomings of a single ingredient without conflicting with each other.

[0028] It should be noted that when the three plant extracts were used simultaneously in this study, it was found that there was a certain antagonistic effect between them. The inventors analyzed the possible reasons for this and solved the possible problems by optimizing the ratio and adjusting the preparation process of hand sanitizer according to the reasons. The brief description is as follows:

[0029] (1) Camellia extract is acidic, while rosemary and angelica extracts are usually neutral or slightly alkaline. If the pH value of the hand sanitizer system is not adjusted properly, it may affect the stability and antibacterial activity of the extract. In view of this, when preparing hand sanitizer, we adjusted its pH value to 5.8-6.2, which not only avoids acid-base interference, but also is closer to the skin pH, which is beneficial to the application effect of hand sanitizer.

[0030] (2) The best antibacterial effect of the three extracts depends on a reasonable ratio. If one component is excessive, it may inhibit the adsorption or activity of other components. In view of this, after many experimental adjustments, we determined that the addition amount of the three extracts in the hand sanitizer was 0.5% (that is, the mass ratio of camellia extract, angelica extract and rosemary extract was 1:1:1). This combination not only makes the addition concentration of the three extracts low, but also has a good application effect, and can avoid the antagonistic effect caused by high concentrations. The comparison of the antibacterial effects of different ratios is shown in Table 1.

[0031] Table 1 Comparison of antibacterial effects of different ratios

[0032] DETAILED DESCRIPTION

[0034] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those generally understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0035] Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art. Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0036] Example 1: Formula of antibacterial hand sanitizer containing plant extracts

[0037] 1. The hand sanitizer of the present invention mainly comprises the following components, wherein the content (mass ratio) and use of each component are as follows:

[0038] (1) Camellia extract: 0.5-1%; antibacterial active ingredient, effectively inhibits common bacteria such as Staphylococcus aureus and Escherichia coli.

[0039] (2) Angelica dahurica extract: 0.5-1%; has antibacterial and anti-inflammatory effects and can effectively inhibit the growth of fungi and bacteria.

[0040] (3) Rosemary extract: 0.5-1%; contains antioxidants, inhibits oxidation reactions, helps stabilize the formula, and also has an antibacterial effect.

[0041] (4) Vitamin E: 1.0%; antioxidant, provides skin protection and moisturizing effects.

[0042] (5) Vegetable glycerin: 5.0%; humectant, provides moisturizing effect.

[0043] (6) Coco-glucoside: 10.0; mild nonionic surfactant.

[0044] (7) Xanthan gum: 0.5; thickener, increases the viscosity of hand sanitizer.

[0045] (8) Deionized water: solvent.

[0046] The mass ratio of camellia extract, angelica dahurica extract and rosemary extract is 1:1:1.

[0047] 2. According to the above ingredients, the formula of the hand sanitizer of the present invention is shown in Table 2 (mass ratio)

[0048] Table 2 Formula of hand sanitizer

[0049] Element content(%) Camellia Extract 0.5 Angelica dahurica extract 0.5 Rosemary Extract 0.5 Vegetable Glycerin 5.0 Vitamin E 1.0 Coco-Glucoside 10.0 Xanthan gum 0.5 Deionized water margin

[0050] Example 2: Preparation of antibacterial hand sanitizer containing plant extracts

[0051] Step 1: Extracting plant active ingredients

[0052] (1) Camellia extract

[0053] Preparation method: crush the dried petals of camellia and place them in a -20℃ environment for pretreatment for 24 hours; take 10 grams of camellia powder and add 150 ml of 25% ethanol solution at a solid-liquid ratio of 1:15; ultrasonic-assisted and microwave-synergistic extraction, the specific conditions are ultrasonic power 300W, frequency 40kHz, microwave power 200W, temperature controlled at 30℃, and extraction time 20 minutes; after the extract is filtered, dynamic countercurrent extraction and purification are carried out, and then low-temperature reduced pressure drying at 40℃ is performed to obtain camellia extract powder.

[0054] Inspection: Through calculation, the powder yield of camellia extract in this study is 9.3%, while the traditional method (using 50% ethanol solution, 1:10 material-liquid ratio, room temperature soaking extraction for 2 hours, and filtering to obtain the extract) is only 4.3%. The tea polyphenol content in the camellia extract prepared in this study was measured by high performance liquid chromatography to be 23.5 mg / g, while the traditional method was only 10.6%. Therefore, compared with the traditional method, the yield of this study is higher.

[0055] (2) Angelica dahurica extract

[0056] Preparation method: Mix 10 grams of dry Angelica dahurica powder with 100 milliliters of 70% ethanol solution in a ratio of 1:10, and extract in a continuous stirring extractor for 30 minutes; filter the extract and concentrate it to a viscous state, place the concentrate in a supercritical CO2 extraction device at a pressure of 30 MPa and a temperature of 40°C for 20 minutes; freeze-dry the extract to obtain Angelica dahurica extract powder.

[0057] Inspection: Through calculation, the powder yield of the Angelica dahurica extract in this study is 8.7%, while the traditional method (using 70% ethanol solution, 1:10 solid-liquid ratio, room temperature soaking extraction for 2 hours, and filtering to obtain the extract) is only 4.5%. The flavonoid content in the Angelica dahurica extract in this study was measured by ultraviolet spectrophotometry to be 10.8 mg / g, while the traditional method was only 4.8%. Therefore, compared with the traditional method, the yield of this study is higher.

[0058] (3) Rosemary Extract

[0059] Preparation method: add 10 grams of rosemary dry powder to 100 milliliters of deionized water in a ratio of 1:10, and add 0.1% sodium citrate as an auxiliary agent; under 45°C water bath conditions, combine with ultrasound with a power of 300W, resonant extraction, and the time is 1 hour; filter the extract and use molecular distillation to remove impurities; freeze-dry to obtain rosemary extract powder.

[0060] Inspection: Through calculation, the powder yield of rosemary extract in this study is 7.8%, while the traditional method (using deionized water, 1:10 solid-liquid ratio, 45℃ water bath extraction for 1 hour, and filtering to obtain the extract) is only 4.2%. Using the DPPH free radical scavenging experiment, the scavenging rate of rosemary extract in this study is 92%, while the traditional method is only 49%. Therefore, compared with the traditional method, the yield of this study is higher.

[0061] (4) Advantages of the extraction method in this study

[0062] Supercritical CO2 extraction and molecular distillation steps are introduced to improve the purity of the extract and the concentration of active ingredients. The retention rate of active ingredients in the extract is improved through low-temperature pretreatment, microwave-assisted ultrasonic extraction, and the use of antioxidant extracting agents. The amount of organic solvents used is reduced, effectively reducing environmental pollution and maintaining the naturalness of plant extracts, making them suitable for use in daily chemical products such as antibacterial hand sanitizers.

[0063] Step 2: Prepare hand sanitizer

[0064] (1) Add 75% deionized water into a mixing container, add the camellia, angelica dahurica and rosemary extracts in sequence, and stir until they are evenly dissolved.

[0065] (2) Add 5% glycerol and 1% vitamin E and continue stirring for 5 minutes.

[0066] (3) Slowly add 10% of coconut glucoside as a mild surfactant and control the stirring speed at 300 rpm to avoid foaming.

[0067] (4) Add 0.5% xanthan gum as a thickener and adjust the viscosity to an appropriate level.

[0068] (5) Adjust the pH value of hand sanitizer to 5.8-6.2 to ensure mildness.

[0069] (6) Place the product in a sterilized container and seal it for storage.

[0070] Example 3: Application research of antibacterial hand sanitizer containing plant extracts

[0071] 1. Antibacterial effect test

[0072] The inhibition zone test was conducted on four common strains of Staphylococcus aureus, Escherichia coli, Candida albicans and Pseudomonas aeruginosa. Each hand sanitizer was tested 5 times and the average value was taken.

[0073] The results show (Table 3) that the formula of the present invention is superior to the single or two plant ingredient formulas in the antibacterial effect on the four strains, and is better than the commercially available hand sanitizer, showing good broad-spectrum antibacterial performance.

[0074] Table 3 Antibacterial effect test results

[0075]

[0076] 2. Skin mildness test

[0077] Ten healthy volunteers were selected and asked to apply hand sanitizer on the inner side of their forearms once a day for seven consecutive days, and any skin irritation or allergic reactions were recorded.

[0078] The results show (Table 4) that the formula of the present invention is mild and non-irritating to the skin, suitable for frequent daily use, and is superior to commercially available hand sanitizers and other single-ingredient or two-ingredient formulas.

[0079] Table 4 Skin mildness test results

[0080]

[0081]

[0082] 3. Moisturizing effect test

[0083] After cleaning their hands, the volunteers applied hand sanitizer and waited for them to dry, and used a skin moisture meter to test the changes in skin moisture content before and after use.

[0084] The results show (Table 5) that the formula of the present invention is significantly better than other formulas in terms of moisturizing effect and can effectively improve the problem of dry skin caused by frequent hand washing.

[0085] Table 5 Moisturizing effect test results

[0086]

[0087] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A natural antibacterial hand sanitizer containing plant extracts, characterized in that: The hand washing liquid comprises the following ingredients: 0.5-1% of camellia extract, 0.5-1% of angelica dahurica extract, 0.5-1% of rosemary extract, 1% of vitamin E, 5% of vegetable glycerin, 10% of coco-glucoside, 0.5% of xanthan gum and the balance of deionized water, wherein the mass ratio of the camellia extract, the angelica dahurica extract and the rosemary extract is 1:1:

1.

2. The antibacterial hand sanitizer according to claim 1, characterized in that The hand washing liquid comprises the following ingredients: 0.5% of camellia extract, 0.5% of angelica extract, 0.5% of rosemary extract, 1% of vitamin E, 5% of vegetable glycerin, 10% of coconut glucoside, 0.5% of xanthan gum and the balance of deionized water.

3. A method for preparing the antibacterial hand sanitizer according to any one of claims 1 or 2, characterized in that: The method comprises the following steps: Step 1: Extracting plant active ingredients, specifically including the following steps: (1) Camellia extract: dried camellia petals were crushed and pretreated at -20°C for 24 hours; 10 g of camellia powder was added to 150 ml of 25% ethanol solution at a solid-liquid ratio of 1:15; ultrasonic-assisted and microwave-assisted extraction was performed, with the specific conditions being an ultrasonic power of 300 W, a frequency of 40 kHz, a microwave power of 200 W, a temperature controlled at 30°C, and an extraction time of 20 minutes; after filtering the extract, dynamic countercurrent extraction and purification were performed, and then low-temperature, reduced-pressure drying was performed at 40°C to obtain camellia extract powder; (2) Angelica dahurica extract: 10 g of dried Angelica dahurica powder was mixed with 100 ml of 70% ethanol solution at a ratio of 1:10, and the mixture was extracted in a continuous stirring extractor for 30 minutes; the extract was filtered and concentrated to a viscous state, and the concentrate was placed in a supercritical CO2 extraction device at a pressure of 30 MPa and a temperature of 40°C for 20 minutes; the extract was freeze-dried to obtain Angelica dahurica extract powder; (3) Rosemary extract: 10 g of rosemary dry powder was added to 100 ml of deionized water at a ratio of 1:10, and 0.1% sodium citrate was added as an auxiliary agent; in a water bath at 45° C., ultrasonic waves were used with a power of 300 W for resonance extraction for 1 hour; the extract was filtered and impurities were removed by molecular distillation; and the extract was freeze-dried to obtain rosemary extract powder; Step 2: Prepare hand sanitizer.

4. The method according to claim 3, characterized in that: The step 2 comprises the following steps: (1) Add 75% deionized water into a mixing container, add camellia, angelica dahurica, and rosemary extracts in sequence, and stir until uniformly dissolved; (2) Add 5% glycerol and 1% vitamin E and continue stirring for 5 minutes; (3) slowly adding 10% coco-glucoside as a mild surfactant, and controlling the stirring speed at 300 rpm to avoid foaming; (4) Add 0.5% xanthan gum as a thickener to adjust the viscosity to an appropriate level; (5) Adjust the pH value of hand sanitizer to 5.8-6.2 to ensure mildness; (6) Place the product in a sterilized container and seal it for storage.

5. Use of the method according to claim 3 in preparing a natural antibacterial hand sanitizer containing plant extracts.

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