Anti-hair loss composition, microcapsule composition, application and cosmetics

Abstract

The invention belongs to the field of daily chemicals, and discloses an anti-hair-loss composition, which comprises 0.1-10 wt% of umbilical cord extract; 0.1 to 3 wt% of a cardiospermum halicacabum extract; and 1-5 wt% of a zucchini seed extract. The composition can further improve the effects of preventing hair loss and growing hair; meanwhile, the formula also has the functions of relieving the scalp and repairing the scalp, and is an effective functional component which has a compound function and can act on the scalp. Meanwhile, the invention also discloses a microcapsule composition containing the composition and application of the composition.

Description

Technical Field

[0001] The invention belongs to the field of new daily chemical materials, and specifically relates to an anti-hair loss composition, a microcapsule composition, an application and a cosmetic. Background Art

[0002] Normally shed hair is in the catagen and telogen phases. Since the hair entering the catagen and the hair entering the new growth phase are constantly in a dynamic balance, the normal amount of hair can be maintained. Pathological hair loss refers to abnormal or excessive hair loss, which has many causes, including but not limited to: androgenic alopecia, neurotic alopecia, endocrine alopecia, nutritional alopecia, physical alopecia, chemical alopecia, infectious alopecia, symptomatic alopecia, etc.

[0003] Most of the existing hair loss products focus on controlling hair loss factors mainly for androgenic alopecia; androgens are converted from cholesterol by enzymes. Testosterone forms dihydrotestosterone through 5α-reductase, and its known mechanisms of causing hair loss are: 1) Dihydrotestosterone binds to androgen receptors and affects the transcription process. 2) Dihydrotestosterone upregulates the synthesis of TGF-β2 in the dermal papilla, and TGF-β2 induces the synthesis and activation of caspases, causing cell apoptosis, advancing the hair regression phase, and shortening the hair growth cycle. 3) Dihydrotestosterone binds to androgen receptors and causes molecular crosstalk with Wnt / β-catenin, hindering the release of Wnt 10b and other factors that are beneficial to hair growth by dermal papilla cells, and releasing immune inflammatory factors such as interleukin 6, affecting the growth and communication of keratinocytes and fibroblasts around the hair follicles.

[0004] For example, the patent application with publication number CN118370806A is a compound hair growth composition for treating hair loss or baldness and its preparation method. It discloses the use of Ligustrum lucidum, Polygonum multiflorum, Perilla frutescens leaves, type 17 collagen, etc. to inhibit hair loss. The main principle is: the synergistic effect of Ligustrum lucidum, Polygonum multiflorum and Perilla frutescens leaves can significantly reduce the secretion of TGF-β1 and promote the growth of new hair follicles; reduce the effect of androgens on susceptible hair follicles, inhibit the miniaturization of hair follicles, and thus reduce hair loss.

[0005] It can be seen that the current mainstream method of preventing hair loss is to inhibit the aging of keratinocytes and fibroblasts around the hair follicles and promote the health of hair follicle cells.

[0006] The applicant's raw material supplier, Guangzhou Youtejia Biotechnology Development Co., Ltd., filed a patent application CN117100673B in 2023. The subject matter is an animal umbilical cord extract for regulating the hair follicle cycle, and its preparation method and application. It discloses that animal umbilical cord extract can be used to regulate the hair follicle cycle, promote the dormant hair follicles to enter the growth phase, and achieve the purpose of preventing hair loss and hair growth.

[0007] After experimenting with the above raw materials, the applicant found that the above raw materials have good effects of preventing hair loss and promoting hair growth. In the actual product formula development process, it is not only required that the product acting on the scalp has the effect of preventing hair loss and promoting hair growth, but also hoped that it can achieve maintenance for the scalp from different dimensions, so as to further improve the application effect of the raw materials. Summary of the invention

[0008] Based on this, the first object of the present invention is to provide an anti-hair loss composition, which can further improve the anti-hair loss and hair growth effects; at the same time, the formula of the present invention also has the functions of scalp soothing and scalp repairing, and is an effective functional ingredient with complex functions that can act on the scalp.

[0009] Meanwhile, the invention also discloses a microcapsule composition containing the composition and application of the composition.

[0010] In order to achieve the first invention object, the present invention adopts the following technical solutions:

[0011] An anti-hair loss composition comprising:

[0012] Umbilical cord extract 0.1-10wt%;

[0013] 0.1-3wt% of linalool extract;

[0014] Zucchini seed extract 1-5wt%.

[0015] The individual effects of each component of the present invention are:

[0016] Umbilical cord extract contains a variety of key components for regulating the physiology of the scalp and hair follicles, such as small molecule proteins, peptides, amino acids, vitamins, etc. It is used to maintain the cell viability of keratinocytes and fibroblasts around the hair follicles and inhibit premature aging. The function of this substance has been comprehensively discussed in the patent application with the publication number CN117100673B, which is entitled "An animal umbilical cord extract for regulating the hair follicle cycle, its preparation method and application", so no further description will be given here.

[0017] The active ingredients in the extract of the Chinese holly are phytosterols, specifically β-sitosterol, stigmasterol, stigmasterol-3-O-β-D-glucoside, etc., which have the function of reducing cholesterol content and reducing inflammation, and are also beneficial to the proliferation of fibroblasts around hair follicles. These give the theoretical possibility that phytosterols can prevent hair loss, and they have the potential to prevent hair loss; at the same time, the extract of the Chinese holly can be used as a plant substitute for hydrocortisone, soothing the skin, instantly relieving itching, reducing inflammation and allergic reactions, and repairing the skin barrier by inhibiting phospholipase A2;

[0018] Cucurbita pekinensis seed extract can upregulate the LC3A gene to activate autophagy. The full name of LC3 is MAP1LC3, which runs through the entire autophagy process and is currently recognized as an autophagy marker. Mammalian LC3 can be divided into three types: LC3A, LC3B and LC3C; LC3A will be modified and processed by ubiquitin-like systems including Atg7 and Atg3, coupled with phosphatidylethanolamine (PE) to form LC3B and localize on the inner and outer membranes of the autophagosome. After the fusion of the autophagosome and the lysosome, the LC3B on the outer membrane is cut by Atg4 to produce LC3A for recycling. The normal dynamic life process of activating cell autophagy, utilizing lysosomal degradation, selectively removing damaged, aging or excess biomacromolecules and organelles, and releasing free small molecules for cell recycling is particularly important for the health of hair follicle-related cells. In addition, in the present invention, the cell repair function of zucchini seed extract makes zucchini seed extract the main component of the formula of the present invention to achieve the scalp repair function.

[0019] Through the above mechanism analysis, it can be seen that the hair follicle cycle regulation function of umbilical cord extract is the core, by further improving the proliferation level of fibroblasts around the hair follicles and improving the repair ability of scalp cells, the number of dormant hair follicles entering the growth phase is further increased, thereby improving the hair follicles' hair fixation ability.

[0020] In further research of the present invention, it is found that by reasonably adjusting the dosage of the above components, 5α-reductase and hyaluronidase can be effectively inhibited. These two indicators are important indicators for achieving oil control and soothing, and are important functional parameters for scalp health. In addition, the formula of the present invention has excellent free radical scavenging ability.

[0021] The formula of the present invention is a composite functional raw material with the core function of preventing hair loss and having the functions of soothing and repairing the scalp.

[0022] It should be noted that the above raw materials are all commercial raw materials, and the amounts used are all the amounts of commercial raw materials. The protection scope of the present invention should be based on the total amount of active ingredients corresponding to the commercial raw materials;

[0023] In the present invention, the content of the active ingredient in the umbilical cord extract is 0.22wt%; the content of the active ingredient in the hollyhock extract is 0.0005wt%; and the content of the active ingredient in the zucchini seed extract is 0.49wt%.

[0024] In the above-mentioned anti-hair loss composition, it includes:

[0025] Umbilical cord extract 4.5-5.5wt%;

[0026] 0.2-1wt% of linalool extract;

[0027] 1.5-2.5wt% of zucchini seed extract.

[0028] In the above-mentioned anti-hair loss composition, it includes:

[0029] Umbilical cord extract 5wt%;

[0030] 0.5wt% of Psoralea corylifolia extract;

[0031] Zucchini seed extract 2wt%.

[0032] Meanwhile, the present invention also discloses a microcapsule composition, comprising a solvent, a phospholipid, and any of the above anti-shedding compositions;

[0033] The microcapsules in the microcapsule composition are liposomes; the shell material of the liposomes includes the phospholipids;

[0034] The weight ratio of the anti-hair loss composition to lecithin is 1.6-18:1-8.

[0035] As is known to all, liposome microcapsules can encapsulate oil-soluble active ingredients and water-soluble active ingredients based on their double-layer structure. They are relatively close to the structure of the skin and have excellent affinity to the skin.

[0036] The present invention adopts liposomes as a specific implementation form of microcapsules, which can improve the delivery uniformity and affinity of the anti-hair loss composition on the scalp and achieve the best effect of the composition.

[0037] As a further improvement of the present invention, the present invention does not use cholesterol as a regulator of the fluidity of the liposome shell material, but instead uses at least part of the plant-derived sterols in the extract of the Herba Lysimachiae as a regulator of the fluidity of the liposome shell material, which, on the one hand, makes the affinity between the liposome and the scalp better, and at the same time, improves the coating stability of the Herba Lysimachiae extract in the liposome, and the release effect on the scalp, and improves the ability to promote the proliferation of fibroblasts around the hair follicles. From the results of human experiments, it has a better anti-hair loss effect than other plant-derived sterols, and has a more significant anti-hair loss effect than liposomes using cholesterol.

[0038] The above microcapsule composition further comprises a solvent, which is polyol or water.

[0039] In the above-mentioned microcapsule composition, the polyol is one or more combinations of butanediol, glycerol, and 1,2-hexanediol;

[0040] Specifically, in the microcapsule composition of the present invention, the water content is 38 to 55 wt %;

[0041] The content of polyol is 40-50wt%;

[0042] The phospholipids are hydrogenated phosphatidylcholine and / or phosphatidylcholine.

[0043] In the above-mentioned microcapsule composition, the shell material of the liposome also includes part or all of the plant-derived sterols in the extract of Cyperus rotundus.

[0044] In the above-mentioned microcapsule composition, the microcapsule composition contains 1.6-18 wt % of the anti-hair loss composition and 1-8 wt % of the phospholipid.

[0045] The microcapsule composition further comprises β-cyclodextrin, and the amount of β-cyclodextrin is 3-7 wt %.

[0046] The main function of β-cyclodextrin is to stabilize the oil-soluble components and form a composite structure of cyclodextrin-Cephala serrata extract-liposomes, more specifically, to form a composite structure of cyclodextrin-oil-soluble components-liposomes;

[0047] The effect of β-cyclodextrin is more obvious when the dosage of the Herba Lycopodii extract is larger. If the dosage of the Herba Lycopodii extract is smaller, it is not recommended to use β-cyclodextrin. β-cyclodextrin is a ring structure with a hydrophobic cavity inside that can accommodate oil-soluble components. When it is loaded with oil-soluble components and coated by liposomes, it can play a role in sustained release and targeting. Therefore, when the dosage of the Herba Lycopodii is lower than 0.5wt%, it is not recommended to use β-cyclodextrin to avoid damaging the fluidity of the liposome shell material.

[0048] In addition, the present invention also discloses the use of any of the above-mentioned microcapsule compositions in preparing cosmetics.

[0049] In the above-mentioned use, the cosmetic is a cosmetic that acts on the scalp and / or hair.

[0050] Finally, the present invention also discloses a cosmetic comprising any one of the above-mentioned microcapsule compositions.

[0051] In the above cosmetics, the cosmetics contain 1 to 20 wt % of the microcapsule composition.

[0052] In the above-mentioned cosmetics, the cosmetics are scalp essence, shampoo or conditioner.

[0053] The beneficial effects of the present invention are:

[0054] The present invention takes the hair follicle cycle regulation function of the umbilical cord extract as the core, further improves the proliferation level of fibroblasts around the hair follicles, improves the repair ability of scalp cells, further increases the number of quiescent hair follicles entering the growth phase, and improves the hair follicle's hair fixation ability. By rationally allocating the dosage of the above components, 5α-reductase and hyaluronidase can be effectively inhibited. These two indicators are important indicators for achieving oil control and soothing, and are an important functional parameter for scalp health. In addition, the formula of the present invention has excellent free radical scavenging ability.

[0055] In summary, the formula of the present invention is a composite functional raw material with the core function of preventing hair loss and having the functions of soothing and repairing the scalp. BRIEF DESCRIPTION OF THE DRAWINGS

[0056] Figure 1 is a flow chart of the experimental steps of the hyaluronidase inhibition assay;

[0057] Figure 2 This is a flow chart of the experimental steps for the ABTS antioxidant effect test;

[0058] Figure 3 This is a photograph of the test results of the cell autophagy test of the microcapsule composition of Example 2. DETAILED DESCRIPTION

[0059] The technical solution of the present invention is further described below by specific implementation methods. It should be understood by those skilled in the art that the embodiments are only to help understand the present invention and should not be regarded as specific limitations of the present invention.

[0060] If no specific experimental steps or conditions are specified in the examples, the conventional experimental steps or conditions described in the literature in the field can be used. If no manufacturer is specified for the reagents or instruments used, they are all conventional reagent products that can be obtained commercially.

[0061] The supplier information and / or product information is as follows:

[0062] (Animal) umbilical cord extract, purchased from Guangzhou Youtejia Biotechnology Development Co., Ltd.; the active ingredient is: mesenchymal stem cells; the content of the active ingredient is: 5.0wt%;

[0063] The extract of Psoralea corylifolia was purchased from Zhuhai Jiayan Biotechnology Co., Ltd.; the active ingredient is phytosterol; the content of the active ingredient is 1.0wt%;

[0064] Zucchini seed extract, purchased from Zhuhai Jiayan Biotechnology Co., Ltd.; the active ingredient is: free amino acids; the content of the active ingredient is: 5.0wt%;

[0065] Camellia seed extract, purchased from Zhuhai Jiayan Biotechnology Co., Ltd.; the active ingredient is: phytosterol; the content of the active ingredient is: 1.0wt%;

[0066] Sunflower seed extract was purchased from Zhuhai Jiayan Biotechnology Co., Ltd.; the active ingredient is phytosterol; the content of the active ingredient is 1.0wt%.

[0067] Part I Preparation of Microcapsule Compositions

[0068] The formulations of the microcapsule compositions of the embodiments and comparative examples of the present invention can be referred to Table 1 and Table 2; the preparation methods thereof are as follows:

[0069] A. Weigh (animal) umbilical cord extract, zucchini seed extract (or camellia seed extract, sunflower seed extract), cyperus rotundus extract, hydrogenated lecithin, water, and polyol according to the following mass percentages;

[0070] B. Mix the extract of the cyperus rotundus and hydrogenated lecithin weighed in step A, heat to 40-60° C., and stir until completely dissolved to prepare the oil phase;

[0071] C. Mix the (animal) umbilical cord extract, zucchini seed extract, water and polyol weighed in step A until completely dissolved, and mix well with β-cyclodextrin;

[0072] D. Mix the oil phase obtained in step B and the phase obtained in step C, add the obtained mixture into a reactor of a supercritical device, first inject CO2 through a CO2 high-pressure pump, adjust the temperature in the reactor of the supercritical device to 30-60° C., set the pressure to 8-16 MPa, incubate for 20-60 min under the supercritical state, release the pressure to release CO2, and cool to room temperature to obtain a microcapsule composition.

[0073] Table 1 Formula table of embodiment

[0074] Example 1 Example 2 Example 3 Example 4 Example 5 (Animal) umbilical cord extract 0.1 5 10 5 5 Extract of linalool 0.5 0.5 3 3 0.5 Zucchini Seed Extract 1 2 5 2 5 Hydrogenated Lecithin 5 5 8 1 5 β-Cyclodextrin 5 5 3 7 5 Butanediol 40 40 40 40 40 glycerin 3 3 3 3 3 1,2-Hexanediol 0.5 0.5 0.5 0.5 0.5 water to 100 to 100 to 100 to 100 to 100

[0075] Table 2 Comparative Example Formula

[0076]

[0077]

[0078] Part II Functional verification of microcapsule composition

[0079] Test Example 1

[0080] Safety test of the microcapsule composition of Examples 1-5

[0081] The microcapsule compositions of Examples 1-5 were subjected to a human skin closed patch test to evaluate their safety.

[0082] The human skin occluded patch test was carried out according to the test requirements for occluded patches in the 2015 edition of the "Technical Specifications for Safety of Cosmetics". The microcapsule compositions of Examples 1-5 were tested; the negative control was pure water. There were 33 subjects in total with an average age of 36.39±6.09 years old. The test results are shown in Table 3.

[0083] Table 3 Different skin reaction scores of the microcapsule composition of Examples 1-5 in the patch test

[0084]

[0085]

[0086] A patch test was performed on 33 subjects using the microcapsule composition. The results showed that all 33 subjects showed negative results after the patches were removed.

[0087] Test Example 2

[0088] 5a-Reductase Inhibition Test of Microcapsule Compositions of Examples 1-5 and Comparative Examples 1-7

[0089] Experimental principle: 5α-reductase is a membrane protease that depends on reduced coenzyme II (NADPH). It is an important male hormone metabolic enzyme in the skin. It can irreversibly convert testosterone (Testosterone, T) into dihydrotestosterone (Dihydrotestosterone, DHT). DHT can induce excessive oil secretion of sebaceous glands. By inhibiting the activity of 5α-reductase to reduce the level of DHT, it can effectively relieve the excessive oil secretion of sebaceous glands.

[0090] Test steps

[0091] In a 10 mL centrifuge tube, PBS, testosterone solution, sample solutions or control solutions of different concentrations, enzyme extract, and NADPH solution were added in sequence, mixed, and incubated at 37°C. The absorbance change value within 10 min was detected at 340 nm using a UV-visible spectrophotometer. The data was analyzed and processed to calculate the inhibition rate of the sample to be tested on 5α-reductase.

[0092] Calculation method

[0093] 5α-reductase inhibition rate I (%) = [(AB) / (AC)] × 100%

[0094] Where: A is the absorbance change value of the normal enzyme reaction group (containing PBS, testosterone solution, enzyme extract solution, and NADPH solution)

[0095] B is the absorbance change value of the experimental group (containing PBS, containing testosterone solution, containing sample solution (sample group) / finasteride solution (positive control group) / water (negative control group), containing enzyme extract solution, containing NADPH solution)

[0096] C is the absorbance change value of the blank group (containing PBS, testosterone solution, and NADPH solution)

[0097] The test results are shown in Table 4 below;

[0098] Table 4 Statistical results of 5a-reductase inhibition rate

[0099]

[0100] Result analysis:

[0101] 1. It can be seen from Examples 1, 2 to 5 that the inhibitory effect of Example 1 on 5a-reductase is lower than that of Examples 2 to 5, the reason being that the active ingredient is relatively low;

[0102] 2. It can be seen from Examples 2 to 5 that the 5a-reductase inhibition rate of Example 2 is the best. The possible reason is that the components in Example 2 have a synergistic effect. In Examples 3 to 5, the amount of active ingredients used is increased, and the synergistic effect between the components decreases.

[0103] 3. It can be seen from the test results of Example 2, Comparative Example 1, Comparative Example 2, Comparative Example 5, Comparative Example 6, and Comparative Example 7 that when other phytosterol extracts (camellia seed extract, sunflower seed extract) are used to replace the Herba Lysimachiae extract, the effect is significantly weaker than that of Example 2; of course, it is undeniable that the Herba Lysimachiae extract is significantly better than the other two in terms of 5a-reductase inhibition rate, but the effect improvement of Example 2 far exceeds the effect brought by the Herba Lysimachiae extract alone. Therefore, we believe that the Herba Lysimachiae extract in this formula is synergistic with other components such as (animal) umbilical cord extract and zucchini seed extract, and at the same time, it has stronger functionality in participating in liposome shell materials.

[0104] 4. It can be seen from Example 2, Comparative Example 3 and Comparative Example 4 that the extract of Psoralea corylifolia and the extract of zucchini seeds are indispensable in the formula of the present invention, and the two work together to comprehensively improve the 5a-reductase inhibition rate.

[0105] Test Example 3

[0106] Hyaluronidase inhibition assay

[0107] Experimental principle: Hyaluronic acid is the most abundant component in the extracellular matrix. It regulates the secretion of cytokines and affects cell adhesion, growth, proliferation and differentiation. Therefore, it plays an important role in maintaining skin moisture and elasticity, wound healing and angiogenesis. Hyaluronidase is a specific cleavage enzyme of hyaluronic acid. It has a strong correlation with the release of histamine by mast cells and is a participant in allergic reactions. Inhibiting the activity of hyaluronidase can ensure the normal content and function of hyaluronic acid. Therefore, the hyaluronidase inhibition rate is used as an indicator to evaluate the soothing effect of cosmetics.

[0108] Hyaluronidase can hydrolyze sodium hyaluronate to generate N-acetylglucosamine, which will condense with p-dimethylaminobenzaldehyde when heated under alkaline conditions to produce a color reaction, and the absorbance value can be measured at 585nm on a spectrophotometer. Samples with hyaluronidase activity inhibition can reduce the generation of chromogenic substances, thereby reducing the absorbance value. The inhibitory effect of the sample on hyaluronidase activity can be evaluated based on the change in absorbance value.

[0109] Test Steps Reference Figure 1 ;

[0110] The test results are shown in Table 5 below;

[0111] Table 5 Statistical table of hyaluronidase inhibition rate results

[0112]

[0113] Result analysis:

[0114] 1. It can be seen from Examples 1 to 5 in Table 5 that the hyaluronidase inhibition rate of each sample can reach more than 60%, among which Examples 3 and 4 have the best effects, indicating that the extract of the Herba Lysimachiae is the main effective ingredient for the soothing effect in the composition of the present invention. The hyaluronic acid inhibition rate of Example 5 can reach 79%, which is significantly improved compared with Example 2, indicating that the zucchini seed extract plays a secondary role in the hyaluronic acid inhibition rate;

[0115] 2. It can be seen from Comparative Examples 5 to 7 in Table 5 that the Herba Lysimachiae extract, Camellia seed extract, and Sunflower seed extract all have a certain hyaluronic acid inhibitory effect. It can be seen from Comparative Examples 1, 2, and Example 2 that the composition of the present invention has a slight advantage in soothing effect. In Comparative Example 3, the amount of Herba Lysimachiae extract was increased to 2.5 g, and its soothing effect was significantly enhanced. The soothing effect of Comparative Example 4 was weaker than that of Comparative Example 3, which further proves that in the composition of the present invention, the Herba Lysimachiae extract plays a major soothing role, and the zucchini seed extract plays a secondary role in the hyaluronic acid inhibition rate.

[0116] Test Example 4

[0117] ABTS Antioxidant Effect Test of Microcapsule Compositions of Examples 1-5 and Comparative Examples 1-7

[0118] Experimental principle: The ABTS method is often used to detect the in vitro antioxidant capacity of substances.

[0119] The stable free radical ABTS+ solution is blue-green and has a maximum absorption at 734nm; this free radical can be generated by the reaction of ABTS and K2S2O8. When the free radical is removed and the number decreases, the color of its solution will become lighter and the absorbance at 734nm will decrease, which can be used to judge the sample's ability to remove ABTS+.

[0120] Test Steps Reference Figure 2 ;

[0121] The test results are shown in Table 6 below;

[0122] Table 6 Statistical results of free radical scavenging rate

[0123]

[0124]

[0125] Result analysis:

[0126] 1. It can be seen from Examples 1 to 5 in Table 6 that each group can show excellent free radical scavenging effect, and the difference between the groups is not significant;

[0127] 2. From Comparative Examples 1 to 4 in Table 6, it can be seen that the free radical scavenging effect of each group is between 67% and 79%, and the difference is not significant;

[0128] 3. It can be seen from Comparative Examples 5 to 7 in Table 6 that the free radical scavenging effect of each group is relatively poor, and the difference is not significant;

[0129] The above cases illustrate that the free radical scavenging effect of the present invention is produced based on the synergistic effect between the components.

[0130] Test Example 5

[0131] Example 2 Anti-shedding test of microcapsule composition (this experiment was sent to a third party for testing, and the testing unit is: Hangzhou Huante Biotechnology Co., Ltd.)

[0132] Experimental principle:

[0133] Hair loss, also known as baldness, is a skin disease that affects appearance and is characterized by hair loss. It is closely related to the number, proliferation, degeneration, and apoptosis of human dermal papilla cells. Human dermal papilla cells (HDPCs) play a core regulatory role in hair growth and hair follicle cycle. They are mesenchymal cells in hair follicles and have the characteristics of adult stem cells. They can affect the development of hair follicles, the hair growth cycle, and the regeneration of hair follicles. Hair papilla cells regulate the proliferation, differentiation, and apoptosis of hair follicle epithelial cells by secreting various growth factors and cell signaling molecules, thereby affecting hair growth and shedding. In terms of anti-hair loss efficacy, promoting the proliferation of hair papilla cells is one of the key factors. Clinical evidence shows that the accumulation of dihydrotestosterone (DHT) in HDPCs is related to androgenic alopecia. 5α-reductase in male testicles converts testosterone (T) in follicles into DHT. DHT regulates male reproductive development, testicular formation, skeletal muscle growth, and hair growth by activating androgen receptors (AR). The affinity of DHT for AR is 10 times that of T, which leads to its overactivation, stimulating HDPCs to secrete TGF-β, causing the hair follicles to transform from the growth phase to the regression phase, shortening the growth phase of hair follicles, and inhibiting the proliferation of hair follicle cells. The nutrition for hair growth requires the supply of capillaries around the hair follicles, and VEGF plays an important role in the formation of capillaries around the hair follicles. VEGF is a highly specific vascular endothelial cell growth factor that promotes angiogenesis, increases vascular permeability, promotes extracellular matrix degeneration, endothelial cell migration, and proliferation. VEGF is overexpressed in keratinocytes of the outer root sheath of hair follicles, which can induce angiogenesis around hair follicles, leading to accelerated hair regeneration after hair removal, increased size of hair follicles and hair shafts, and increased hair density. The Wnt / β-catenin pathway has a positive effect on the growth and circulation of mammalian hair follicles. After activation, β-catenin accumulates in the cytoplasm and then translocates to the nucleus, where it interacts with Lef / Tcf transcription factors to regulate the expression of genes responsible for hair follicle growth. DHT can inhibit the Wnt / β-catenin pathway in human dermal papilla cells. DKK1 (Dickkopf-1) is a secreted glycoprotein belonging to the DKK family. It competitively binds to the LRP5 / 6 receptor with the Wnt protein, antagonizes the activity of the Wnt / β-catenin signaling pathway, regulates cell proliferation and differentiation, and is a typical inhibitor of the Wnt / β-catenin signaling pathway.

[0134] Therefore, this experiment used human dermal papilla cells to construct an experimental model. By detecting the promoting effect of the samples on cell proliferation and the relative expression levels of AR, TGF-β, DDK1, VEGF, and β-catenin genes after the samples acted on the cells, we determined whether the samples had anti-hair loss effects in multiple dimensions.

[0135] Experimental steps:

[0136] 1. Cell proliferation

[0137] 1. Inoculate cells in a 96-well plate (5×103 cells / well) and incubate at 37°C, 5% CO2 for 24 h.

[0138] 2. Administration: Add fresh culture medium containing samples of corresponding concentrations to the sample group, and replace with fresh medium to the normal control group.

[0139] The blank group was added with blank culture medium and incubated at 37°C, 5% CO2 for 48 h.

[0140] 3. After incubation, add MTT solution to each well and continue culturing for 4 hours.

[0141] 4. Remove the culture medium, add DMSO solution, shake to mix, and measure the absorbance at 490nm.

[0142]

[0143] OD490: absorbance value at 490nm

[0144] II. Relative expression of AR, TGF-β, DDK1, VEGF, and β-catenin genes

[0145] 1. Inoculate cells in 6-well plates (3×105 cells / well) and incubate at 37°C, 5% CO2 for 24 h.

[0146] 2. Administration: After the incubation, fresh complete medium was added to the normal control group, fresh complete medium containing (30 μM DHT) was added to the model control group, fresh complete medium containing (30 μM DHT + sample) was added to the sample group, and fresh complete medium containing (30 μM DHT + 400 μM minoxidil) was added to the positive control group, and incubated at 37°C, 5% CO2 for 24 h.

[0147] 3. Collect cells, extract total RNA from each experimental group, synthesize cDNA, and use q-PCR to detect the gene expression of β-actin and target genes.

[0148] 4. Use β-actin as an internal reference for gene expression and calculate the relative RNA expression of the target gene.

[0149] Relative RNA expression = 2 ΔΔC(t)

[0150]

[0151] AC(t)=C(t) 目的基因 -C(t)β-actin

[0152] The test results are shown in Table 7 below:

[0153] Table 7 Detection results of relative expression of AR, TGF-β, DDK1, VEGF, β-catenin genes

[0154]

[0155] Summary: The cell survival rate of the microcapsule composition group of Sample Example 2 was significantly increased compared with the normal control group; the relative expression levels of AR, TGF-β, and DDK1 genes were significantly decreased compared with the model control group, and the relative expression levels of VEGF and β-catenin genes were significantly increased compared with the model control group, revealing that the sample has anti-hair loss effect.

[0156] Test Example 6

[0157] Cell autophagy test of the microcapsule composition of Example 2 (this experiment was sent to a third party for testing, and the testing unit is: Hangzhou Huante Biotechnology Co., Ltd.)

[0158] Experimental principle:

[0159] Autophagy is a self-protection mechanism that is prevalent in eukaryotic cells. Cells have aged and damaged organelles, incorrectly synthesized proteins, bacteria that invade cells, and macromolecules. Autophagy forms autophagosomes to encapsulate these substances that are harmful to cells, and combines with lysosomes to form autophagosomes to decompose and remove these substances, maintaining the homeostasis of the intracellular environment and the recycling of intracellular substances. The expression of the autophagy-related protein LC3Ⅱ can be detected by Western blotting to determine whether the level of autophagy has increased.

[0160] Test steps:

[0161] 1. Inoculate cells in 6-well plates (3×105 cells / well) and incubate at 37°C, 5% CO2 for 24 h.

[0162] 2. After the incubation, remove the culture medium, add fresh culture medium containing the sample to the sample group, replace the normal control group with fresh culture medium, and add fresh culture medium containing 50nM rapamycin to the positive control group, and continue to incubate at 37°C, 5% CO2 for 24h.

[0163] 3. After incubation, use RIPA lysis buffer to lyse the cells and extract protein.

[0164] 4. Detect LC3Ⅱ protein expression by Western Blot method.

[0165] The test results are shown in Table 8 and Figure 3 As shown;

[0166] Table 8 Statistics of cell autophagy test results

[0167] Test Group Detection concentration LC3II / β-actin ratio effect(%) P-value Test results Normal control group / 0.942 / - - Example 2 Microcapsule composition 0.03125％ 1.25 33 ＜0.05 Significant Rapamycin 50nM 1.66 76 ＜0.01 Significant

[0168] Summary: Under the experimental conditions of this study, the microcapsule composition of Sample Example 2 has the effect of promoting cell autophagy.

[0169] Part 4 Preparation of scalp serum and anti-hair loss test on human body

[0170] The formula of the scalp essence is shown in Table 9; the preparation method is as follows:

[0171] Step 1: Mix phase A in a clean and dry container and add phase B for premixing;

[0172] Step 2: Start homogenization, add phase C into the pot, stop homogenization, stir and heat to 80℃-85℃, keep warm for 30 minutes;

[0173] Step 3: Start cooling device, the temperature drops to 40-45°C, add phase A and D, and stir evenly until transparent and clear;

[0174] Step 4: Take the intermediate product to test the pH. If the pH value is higher than the standard range (pH 5-7), add phase E;

[0175] Step 5: Stir evenly to obtain the scalp essence product.

[0176] Table 9 Scalp Essence Formula

[0177]

[0178] Eight scalp essence samples were obtained by the formula in Table 9, and the corresponding relationship between their numbers and the microcapsule compositions used is shown in Table 10;

[0179] Table 10 Information table of scalp serum

[0180]

[0181]

[0182] Test Example 7

[0183] Example 2, Comparative Example 1 Microcapsule composition scalp essence human body test

[0184] Experimental steps:

[0185] 1. Materials and Methods

[0186] 1.1. Test products: Scalp Essence 1 (Example 2), Scalp Essence 2 (Comparative Example 1).

[0187] 1.2. Control product: A product with a base formula of the corresponding test product that does not contain anti-hair loss ingredients and is tested in parallel with the test product.

[0188] 1.3. Wash-out products: Same as the control products. Both the test group and the control group use the control products during the wash-out period.

[0189] 1.4. Subjects: A total of 90 subjects completed the test, aged 24 to 53 years old. The experimental group was divided into 2 groups, 30 people in each group, 26 males and 34 females in total, and the male-female ratio was adjusted to a balanced state in each experimental group; the control group had 30 people, 12 males and 18 females. The subjects met the voluntary inclusion and exclusion criteria.

[0190] 1.5 Test method: Test in accordance with the specific requirements of the "Technical Specifications for Safety of Cosmetics" (2015 edition). Volunteer subjects were recruited according to the inclusion and exclusion criteria. Qualified subjects underwent a 2-week washout period. After the washout period, they were combed 60 times again. Only those with a hair loss count of more than 10 hairs could enter the formal test. Subjects were divided into the test product group and the control product group according to the stratified random method, and the test product and the control product were distributed respectively. The product was used correctly for 12 weeks according to the product instructions. During the trial, the subjects were required to keep a usage diary and strictly abide by the restrictions. The subjects were visited before use (basic value) and 4 weeks, 8 weeks, and 12 weeks after use. The hair loss count was counted and recorded at each visit. At the same time, the subjects were tested for scalp physiological parameters, stratum corneum moisture content, transepidermal water loss rate, oil content, scalp red area, and elasticity value before use, 2 weeks, and 4 weeks.

[0191] The test results can be seen in Table 11;

[0192] Table 11 Human experimental test results

[0193]

[0194]

[0195] Result analysis:

[0196] Through the continuous use of the product for 84 days by the subjects, it can be seen that the moisture content of the scalp stratum corneum of the test group Essence 1 was significantly improved by 59.88%, the transepidermal water loss rate was significantly improved by 25.05%, the oil content was significantly improved by 25.07%, and the scalp elasticity value was significantly improved by 13.82% at the 4-week follow-up. At the 12-week follow-up, the hair loss count of the test group Essence 1 was significantly improved by 59.06%, and the local hair density increased by 4.56%, indicating that the test product Essence 1 has the effect of soothing, repairing the scalp, and preventing hair loss for people with hair loss. The test group Essence 2 has an improved effect compared with the control group, but the improvement effect is not ideal, indicating that the extract of the cyperus rotundus has a reinforcing effect in preventing hair loss.

[0197] In summary, the microcapsule composition of the anti-hair loss composition of the present invention has obvious effects of preventing hair loss and strengthening hair, promoting hair growth, soothing and repairing the scalp barrier.

[0198] The embodiments presented herein are only embodiments selected according to the combination of all possible embodiments. The appended claims should not be limited by the embodiments describing the present invention. Some numerical ranges used in the claims include sub-ranges therein, and the changes in these ranges should also be covered by the appended claims.

Claims

1. An anti-shedding composition, characterized in that include: Umbilical cord extract 0.1-10wt%; 0.1-3wt% of linalool extract; Zucchini seed extract 1-5wt%.

2. The anti-shedding composition according to claim 1, characterized in that include: Umbilical cord extract 4.5-5.5wt%; 0.2-1 wt% of linalool extract; 1.5-2.5wt% of zucchini seed extract.

3. A microcapsule composition, characterized in that: Comprising a solvent, a phospholipid, and the anti-hair loss composition according to claim 1 or 2; The microcapsules in the microcapsule composition are liposomes; the shell material of the liposomes includes the phospholipids; The weight ratio of the anti-hair loss composition to lecithin is 1.6-18:1-8.

4. The microcapsule composition according to claim 3, characterized in that It also includes a solvent, wherein the solvent is a polyol or water; the polyol is one or more combinations of butanediol, glycerol, and 1,2-hexanediol; and the phospholipid is hydrogenated lecithin and / or lecithin.

5. The microcapsule composition according to claim 3, characterized in that: The shell material of the liposome also includes part or all of the plant-derived sterols in the extract of the Herba Lysimachiae, and the microcapsule composition contains 1.6-18 wt % of the anti-hair loss composition and 1-8 wt % of phospholipids.

6. The microcapsule composition according to claim 3, characterized in that It also includes β-cyclodextrin, and the amount of the β-cyclodextrin is 3-7wt%.

7. Use of the microcapsule composition according to any one of claims 3 to 6 for preparing cosmetics, wherein the cosmetics are cosmetics for use on the scalp and / or hair.

8. A cosmetic, characterized in that: A microcapsule composition comprising the microcapsule composition as claimed in any one of claims 3 to 6.

9. The cosmetic according to claim 8, characterized in that: The cosmetic contains 1-20 wt% of the microcapsule composition.

10. The cosmetic according to claim 8, characterized in that: The cosmetic is scalp essence, shampoo or conditioner.

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