Composition for removing and clearing internal heat, application of composition, toothpaste containing composition and preparation method of toothpaste

Abstract

The invention discloses a composition for removing and clearing internal heat, application of the composition, toothpaste containing the composition and a preparation method, and belongs to the technical field of biological medicine and daily chemical products. The composition disclosed by the invention is prepared from 0.1 to 0.5 part of blumea balsamifera oil, 0.1 to 0.5 part of blumea balsamifera, 0.1 to 0.5 part of peppermint oil, 0.05 to 0.2 part of eugenol, 0.1 to 0.5 part of menthol and 0.1 to 0.5 part of essence. The invention discloses an application of the composition in preparation of oral cavity products and toothpaste containing the composition. The composition is scientific in design and ingenious in conception, has a good bacteriostatic effect, can effectively grow dental plaque and remove ozostomia, and further has the effects of resisting inflammation, diminishing inflammation and relieving pain.

Description

Technical Field

[0001] The invention belongs to the technical field of biomedicine and daily chemical products, and particularly relates to a composition for removing heat and clearing internal heat, an application thereof, a toothpaste containing the composition and a preparation method thereof. Background Art

[0002] Traditional Chinese medicine believes that when the yin and yang of the human body are out of balance, the internal fire is strong, which is called getting angry. The so-called "fire" describes certain hot symptoms in the body. Getting angry is the internal heat syndrome that occurs when the yin and yang of the human body are out of balance. Specific symptoms include red and swollen eyes, erosion at the corners of the mouth, yellow urine, toothache, sore throat, etc. The solution is to remove and clear the fire, that is, to adopt the principles and methods of traditional Chinese medicine to control fire with bitter and cold, clear heat and detoxify, and purge the excess and reduce fire. Yin-nourishing, heat-clearing, detoxifying and swelling-reducing drugs can be used.

[0003] Dental plaque is a group of soft, unmineralized bacteria wrapped in a matrix that adheres to each other, or to the tooth surface, between teeth, or on the surface of restorations. It is a bacterial biofilm that cannot be washed away or rinsed off with water. Dental plaque can cause caries and periodontal disease. Bad breath refers to the odor emitted from the mouth or other air-filled cavities such as the nose, sinuses, and pharynx. According to statistics, 80 to 90 percent of bad breath comes from the mouth. Untreated caries, residual roots, residual crowns, poor restorations, abnormal anatomical structures, gingivitis, periodontitis, and oral mucosal diseases in the mouth can all cause bad breath. In addition, oral diseases are often accompanied by inflammation and toothache symptoms.

[0004] In the prior art, it is generally advocated to inhibit the growth of dental plaque and reduce bad breath by cleaning the oral cavity in daily life. However, when oral diseases occur, the toothpaste used in daily life has limited effect; and it cannot treat or relieve the inflammation and toothache symptoms associated with the disease. In addition, oral drugs are generally used in the prior art to remove fire and clear fire to relieve or treat oral fire symptoms, and there is no oral cleaning product that can play the role of removing fire and clearing fire when cleaning the oral cavity. Therefore, a composition for removing fire and clearing fire is provided, which can not only clean the oral cavity, effectively inhibit the growth of dental plaque, remove bad breath, and remove fire and clear fire, and has anti-inflammatory, anti-inflammatory and analgesic effects, which has become a problem to be solved by those skilled in the art. Summary of the invention

[0005] One of the purposes of the present invention is to provide a composition for removing heat and clearing internal heat, which not only has an antibacterial effect, can effectively inhibit the growth of dental plaque, and remove bad breath, but also can remove heat and clear internal heat, resist inflammation, reduce inflammation and relieve pain.

[0006] The second object of the present invention is to provide an application of the composition.

[0007] The third object of the present invention is to provide a toothpaste comprising the composition.

[0008] A fourth object of the present invention is to provide a method for preparing the toothpaste.

[0009] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0010] The invention discloses a composition for removing heat and clearing internal heat, comprising the following components in parts by weight: 0.1-0.5 parts of mugwort oil, 0.1-0.5 parts of mugwort slices, 0.1-0.5 parts of peppermint oil, 0.05-0.2 parts of eugenol, 0.1-0.5 parts of menthol and 0.1-0.5 parts of essence.

[0011] The invention comprises 0.1-0.5 parts of Blumea balsamifera oil, for example, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5 parts.

[0012] The present invention comprises 0.1-0.5 parts of moxa tablets, for example 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5 parts.

[0013] The present invention comprises 0.1-0.5 parts of peppermint oil, for example 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5 parts.

[0014] The present invention comprises 0.05-0.2 parts of eugenol, for example, 0.05, 0.07, 0.09, 0.1, 0.11, 0.13, 0.15, 0.17, 0.19, 0.2 parts.

[0015] The present invention comprises 0.1-0.5 parts of menthol, for example 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5 parts.

[0016] The present invention comprises 0.1-0.5 parts of flavor, for example 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5 parts.

[0017] In some embodiments of the present invention, the composition includes the following components in parts by weight: 0.2 parts of mugwort oil, 0.2 parts of moxa slices, 0.2 parts of peppermint oil, 0.1 parts of eugenol, 0.2 parts of menthol, and 0.2 parts of essence.

[0018] In some embodiments of the present invention, the Blumea balsamifera oil is the volatile oil of Blumea balsamifera, which is the leaves and young branches of the Blumea balsamifera plant of the Asteraceae family.

[0019] The mint oil is volatile oil extracted from fresh stems and leaves of Mentha haplocalyx Briq., a plant of the Lamiaceae family, through steam distillation, freezing and partial brain removal.

[0020] The mugwort slices of the present invention are crystals prepared by extracting and processing fresh leaves of Blumea balsamifera (L.) DC., a plant of the Asteraceae family.

[0021] The flavor of the present invention includes a single flavor or a compound flavor.

[0022] The essence in the present invention is used as a flavoring agent or / and a flavoring agent to improve the taste and / or aroma of the paste. The essence in the present invention is the prior art, including strawberry essence, apple essence, sandalwood essence, jasmine essential oil, orange essential oil, snow mountain tea, lemon cedar, etc. According to different product requirements, essences with different fragrances and tastes are selected. The application of the above-mentioned composition disclosed in the present invention in the preparation of oral products.

[0023] In some embodiments of the present invention, the application is in the preparation of oral products for inhibiting dental plaque and / or removing bad breath and / or removing internal heat and / or resisting inflammation and / or relieving pain.

[0024] In some embodiments of the present invention, the oral product comprises a paste, preferably a toothpaste.

[0025] The present invention discloses a toothpaste comprising the above-mentioned composition, wherein the toothpaste further comprises 20-30 parts of an abrasive;

[0026] Moisturizer 26-40 parts;

[0027] Thickener 0.6-2 parts;

[0028] 1.6-4 parts of surfactant;

[0029] Flavoring agent 0.1-0.3 parts;

[0030] Preservative 0.1-0.4 parts;

[0031] pH regulator 0.01-0.5 parts;

[0032] 25-35 parts of water.

[0033] In some embodiments of the present invention, the friction agent includes silica, preferably MII type silica or / and H silica;

[0034] The moisturizing agent includes at least one of sorbitol and propylene glycol;

[0035] The thickener comprises at least one of sodium carboxymethyl cellulose and / or xanthan gum;

[0036] The surfactant includes at least one of sodium lauryl sulfate, cocamidopropyl betaine, and PEG-40 hydrogenated castor oil;

[0037] The flavoring agent includes sodium saccharin;

[0038] The preservatives include sodium benzoate;

[0039] The pH adjuster is an alkaline substance, preferably sodium bicarbonate.

[0040] The essence in the present invention is mainly used for adjusting the taste and aroma.

[0041] The preparation method of the toothpaste disclosed by the invention comprises the following steps: weighing each component in proportion and mixing them evenly to obtain the toothpaste.

[0042] Some embodiments of the present invention include the following steps:

[0043] Step 1. Prepare an effective component liquid: mix moxa slices, menthol, and part of the surfactant evenly, then add eugenol, Blumea balsamifera, peppermint oil and essence to the mixture, mix evenly, and obtain an effective component liquid; preferably, the surfactant in step 1 is PEG-40 hydrogenated castor oil;

[0044] Step 2. Prepare a liquid material: mix water, a moisturizer, a preservative, a flavoring agent, and a pH adjuster, stir until dissolved, and then mix with the liquid surfactant in the remaining surfactant to obtain a liquid material; preferably, the liquid surfactant in step 2 is cocamidopropyl betaine;

[0045] Step 3. Prepare powder: mix the friction agent, thickener and solid surfactant evenly to obtain powder; preferably, the solid surfactant in step 3 is sodium dodecyl sulfate;

[0046] Step 4: After the liquid material and the powder material are evenly mixed, they are evenly mixed with the functional component liquid to obtain the toothpaste.

[0047] Compared with the prior art, the present invention has the following beneficial effects:

[0048] The invention is scientifically designed and ingeniously conceived. The composition of the invention has a good antibacterial effect. Tests show that the antibacterial rates of the toothpaste made of the composition of the invention on Porphyromonas gingivalis, Streptococcus mutans, Fusobacterium nucleatum and Lactobacillus acidophilus are all greater than 99.99%. In addition, the invention can effectively inhibit the growth of dental plaque, remove bad breath, and also has the effects of anti-inflammatory, anti-inflammatory and analgesic.

[0049] The raw materials of the invention are easily available, the production method is simple, and industrial production can be easily realized. DETAILED DESCRIPTION

[0050] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be described clearly and completely below. If no specific conditions are specified in the embodiments, the conventional conditions or the conditions recommended by the manufacturer are followed. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially. Example 1

[0051] This embodiment discloses a method for preparing a toothpaste composition for removing internal heat and clearing internal heat of the present invention. The raw materials of the toothpaste are shown in the following table:

[0052] Table 1

[0053]

[0054]

[0055] The specific preparation method is as follows:

[0056] 1. Weigh the raw materials of phase A according to the proportion, pour them into the liquid premixing pot and stir for 5 minutes until the raw materials are completely dissolved to obtain liquid;

[0057] 2. Put all the raw materials of phase B1 into the powder premixing pot and stir for 10 minutes to obtain powder;

[0058] 3. Add phase B2 into the paste making machine;

[0059] 4. Turn on the vacuum machine and draw the vacuum degree in the paste making machine to ≥-0.06MPa; open the liquid feed valve and feed liquid into the paste making machine. After the liquid is fed in, close the liquid feed valve.

[0060] 5. Turn on the stirring, scraper and homogenizer, keep the vacuum degree in the paste making machine at -0.065-0.085MPa, open the powder feed valve to feed the powder into the paste making machine, and close the powder feed valve after the powder is fed;

[0061] 6. After adding the liquid and powder materials, reduce the vacuum degree of the paste making machine to -0.096MPa, turn off the vacuum machine, and stir for 20 minutes.

[0062] 7. All the raw materials of phase C1 are dissolved in a water bath at about 85°C. Then cool to below 45°C, add phase C2, stir evenly, and suck into the paste making machine through the flavor feed port; after feeding, close the flavor feed valve.

[0063] 9. 5 minutes after entering phase C1 and phase C2, turn on the vacuum pump to degas. When there are no obvious bubbles, notify the quality control department to inspect the paste. The paste will be discharged after passing the inspection.

[0064] The paste obtained in this embodiment is colorless and translucent, has no foreign matter, and has a pH value of 5.5-10.

[0065] Example 2

[0066] This embodiment discloses a method for preparing a toothpaste composition for removing internal heat and clearing internal heat of the present invention. The raw materials of the toothpaste are shown in the following table:

[0067] Table 2

[0068]

[0069]

[0070] The specific preparation method is as follows:

[0071] 1. Weigh the raw materials of phase A according to the proportion, pour them into the liquid premixing pot and stir for 8 minutes until the raw materials are completely dissolved to obtain liquid;

[0072] 2. Put all the raw materials of phase B1 into the powder premixing pot and stir for 12 minutes to obtain powder;

[0073] 3. Add phase B2 into the paste making machine;

[0074] 4. Turn on the vacuum machine and draw the vacuum degree in the paste making machine to ≥-0.06MPa; open the liquid feed valve and feed liquid into the paste making machine. After the liquid is fed in, close the liquid feed valve.

[0075] 5. Turn on the stirring, scraper and homogenizer, keep the vacuum degree in the paste making machine at -0.065-0.085MPa, open the powder feed valve to feed the powder into the paste making machine, and close the powder feed valve after the powder is fed;

[0076] 6. After adding the liquid and powder materials, reduce the vacuum degree of the paste making machine to -0.1MPa, turn off the vacuum machine, and stir for 25 minutes.

[0077] 7. All the raw materials of phase C1 are dissolved in a water bath at about 80℃. Then cool to below 40℃, add phase C2, stir evenly, and suck into the paste making machine through the flavor feed port; after feeding, close the flavor feed valve.

[0078] 9. 8 minutes after entering phase C1 and phase C2, turn on the vacuum pump to degas. When there are no obvious bubbles, notify the quality control department to inspect the paste. The paste will be discharged after passing the inspection.

[0079] The paste obtained in this embodiment is colorless and translucent, has no foreign matter, and has a pH value of 5.5-10.

[0080] Example 3

[0081] This embodiment discloses a method for preparing a toothpaste composition for removing internal heat and clearing internal heat of the present invention. The raw materials of the toothpaste are shown in the following table:

[0082] Table 3

[0083]

[0084]

[0085] The specific preparation method is as follows:

[0086] 1. Weigh the raw materials of phase A according to the proportion, pour them into the liquid premixing pot and stir for 5 minutes until the raw materials are completely dissolved to obtain liquid;

[0087] 2. Put all the raw materials of phase B1 into the powder premixing pot and stir for 10 minutes to obtain powder;

[0088] 3. Add phase B2 into the paste making machine;

[0089] 4. Turn on the vacuum machine and draw the vacuum degree in the paste making machine to ≥-0.06MPa; open the liquid feed valve and feed liquid into the paste making machine. After the liquid is fed in, close the liquid feed valve.

[0090] 5. Turn on the stirring, scraper and homogenizer, keep the vacuum degree in the paste making machine at -0.065-0.085MPa, open the powder feed valve to feed the powder into the paste making machine, and close the powder feed valve after the powder is fed;

[0091] 6. After adding the liquid and powder materials, reduce the vacuum degree of the paste making machine to -0.090MPa, turn off the vacuum machine, and stir for 20 minutes.

[0092] 7. All the raw materials of phase C1 are dissolved in a water bath at about 85°C. Then cool to below 45°C, add phase C2, stir evenly, and suck into the paste making machine through the flavor feed port; after feeding, close the flavor feed valve.

[0093] 9. 10 minutes after entering phase C1 and phase C2, turn on the vacuum pump to degas. When there are no obvious bubbles, notify the quality control department to inspect the paste. The paste will be discharged after passing the inspection.

[0094] The paste obtained in this embodiment is colorless and translucent, has no foreign matter, and has a pH value of 5.5-10.

[0095] Example 4

[0096] This embodiment discloses a method for preparing a toothpaste composition for removing internal heat and clearing internal heat of the present invention. The raw materials of the toothpaste are shown in the following table:

[0097] Table 4

[0098]

[0099] The specific preparation method is as follows:

[0100] 1. Weigh the raw materials of phase A according to the proportion, pour them into the liquid premixing pot and stir for 5 minutes until the raw materials are completely dissolved to obtain liquid;

[0101] 2. Put all the raw materials of phase B1 into the powder premixing pot and stir for 5 minutes to obtain powder;

[0102] 3. Add phase B2 into the paste making machine;

[0103] 4. Turn on the vacuum machine and draw the vacuum degree in the paste making machine to ≥-0.05MPa; open the liquid feed valve and feed liquid into the paste making machine. After the liquid is fed in, close the liquid feed valve.

[0104] 5. Turn on the stirring, scraper and homogenizer, keep the vacuum degree in the paste making machine at -0.065-0.090MPa, open the powder feed valve to feed the powder into the paste making machine, and close the powder feed valve after the powder is fed;

[0105] 6. After adding the liquid and powder materials, reduce the vacuum degree of the paste making machine to -0.096MPa, turn off the vacuum machine, and stir for 20 minutes.

[0106] 7. All the raw materials of phase C1 are dissolved in a water bath at about 85°C. Then cool to below 40°C, add phase C2, stir evenly, and suck into the paste making machine through the flavor feed port; after feeding, close the flavor feed valve.

[0107] 9. 5 minutes after entering phase C1 and phase C2, turn on the vacuum pump to degas. When there are no obvious bubbles, notify the quality control department to inspect the paste. The paste will be discharged after passing the inspection.

[0108] The paste obtained in this embodiment is colorless and translucent, has no foreign matter, and has a pH value of 5.5-10.

[0109] Example 5

[0110] This embodiment discloses a method for preparing a toothpaste composition for removing internal heat and clearing internal heat of the present invention. The raw materials of the toothpaste are shown in the following table:

[0111] Table 5

[0112]

[0113] The specific preparation method is as follows:

[0114] 1. Weigh the raw materials of phase A according to the proportion, pour them into the liquid premixing pot and stir for 5 minutes until the raw materials are completely dissolved to obtain liquid;

[0115] 2. Put all the raw materials of phase B1 into the powder premixing pot and stir for 10 minutes to obtain powder;

[0116] 3. Add phase B2 into the paste making machine;

[0117] 4. Turn on the vacuum machine and draw the vacuum degree in the paste making machine to ≥-0.05MPa; open the liquid feed valve and feed liquid into the paste making machine. After the liquid is fed in, close the liquid feed valve.

[0118] 5. Turn on the stirring, scraper and homogenizer, keep the vacuum degree in the paste making machine at -0.065-0.090MPa, open the powder feed valve to feed the powder into the paste making machine, and close the powder feed valve after the powder is fed;

[0119] 6. After adding the liquid and powder materials, reduce the vacuum degree of the paste making machine to -0.096MPa, turn off the vacuum machine, and stir for 20 minutes.

[0120] 7. All the raw materials of phase C1 are dissolved in a water bath at about 85°C. Then cool to below 45°C, add phase C2, stir evenly, and suck into the paste making machine through the flavor feed port; after feeding, close the flavor feed valve.

[0121] 8. 5 minutes after entering phase C1 and phase C2, turn on the vacuum pump to degas. When there are no obvious bubbles, notify the quality control to inspect the paste. The paste will be discharged after passing the inspection.

[0122] The paste obtained in this embodiment is colorless and translucent, has no foreign matter, and has a pH value of 5.5-10.

[0123] Test Example 1

[0124] This test example discloses an antibacterial test of the toothpaste for removing heat and clearing internal heat of the present invention. The toothpaste for removing heat and clearing internal heat used in this test example is prepared according to the method of Example 5.

[0125] The method of this test example is carried out in accordance with WS / T 650-2019 "Evaluation Methods for Antibacterial and Antibacterial Effects". The results are shown in the following table:

[0126] Table 6

[0127]

[0128] Test Example 2-Test Example 5

[0129] Test Examples 2 to 5 were respectively subjected to routine testing, halitosis removal testing, anti-inflammatory testing and dental plaque inhibition testing on the fire-clearing toothpaste of the present invention. Test Examples 2 to 5 were completed by Guangzhou Quality Supervision and Inspection Institute and National Cosmetics Quality Inspection and Testing Center (Guangzhou).

[0130] The fire-clearing toothpaste of the present invention used in Experimental Examples 2 to 5 was prepared according to the method of Example 5.

[0131] Test Example 2

[0132] This test example discloses the conventional test results of the fire-clearing toothpaste of the present invention, as shown in the following table:

[0133] Table 7 Conventional test results

[0134]

[0135] Test Example 3

[0136] This test example discloses the test of removing bad breath by the fire-clearing toothpaste of the present invention. The details are as follows:

[0137] 1. Experimental Grouping

[0138] Sample group: the fire-clearing toothpaste of the present invention

[0139] Control group: ordinary toothpaste

[0140] 2. Experimental Methods

[0141] 1) Cultivation of membrane: Collect 14g of saliva from 4 volunteers and mix them in 50mL centrifuge tubes. Take 10 10mL centrifuge tubes, put a hydroxyapatite (HAP) sheet and 1mL of saliva in each centrifuge tube, and then place the centrifuge tube in a constant temperature water bath at 37℃ for overnight culture to form an acquired membrane on the surface of the HAP sheet. Take the centrifuge tube out of the water bath the next day and remove the saliva.

[0142] 2) Sample preparation: toothpaste and water were thoroughly mixed in a mass ratio of 1:1.6 to form a uniform slurry.

[0143] 3) Treatment of test samples: 10 cultured HAP sheets were randomly divided into a sample group and a control group, with 5 sheets in each group. The HAP sheets in the sample group were soaked in the sample toothpaste slurry for 30 seconds, 5 mL of toothpaste slurry per sheet, and then taken out and placed in a headspace bottle, with 1 sheet in each bottle. The control group was treated in the same way with ordinary toothpaste slurry.

[0144] 4) Preparation of saliva culture medium solution: Weigh 0.75g of thioglycollate culture medium, add 25g of deionized water, heat and stir to dissolve as culture medium stock solution. Collect 26g of saliva from 4 volunteers and mix them in a 50mL centrifuge tube. Prepare saliva culture medium solution according to the ratio of culture medium stock solution: deionized water: saliva (1.6:13.4:85), mix well and set aside.

[0145] 5) Cultivation and gas production: Add 3 mL of saliva culture medium solution to each headspace bottle, seal it and place it in a 37°C water bath for 12 hours to produce volatile sulfide gas. Take the incubated headspace bottle out of the water bath the next day.

[0146] 6) Quantitative detection of methyl mercaptan: The headspace bottle is placed in a headspace sampler, and the peak area of ​​methyl mercaptan in the gas in the headspace bottle is measured by gas chromatography-flame photometric detector, and the logarithm of the peak area is calculated.

[0147] 3. Evaluation Method

[0148] SPSS27.0 statistical software was used to test the normality of the logarithm of the peak area using Shapiro-Wilk. If P>0.05, it was normally distributed, and a t test was performed with a significance level of a = 0.05. If P<0.05, it was non-normally distributed, and a Wilcoxon rank sum test was performed with a significance level of a. = 0.05.

[0149] Methyl mercaptan is one of the characteristic substances of breath. The less methyl mercaptan, the better the effect of removing bad breath. If the value of the sample group is less than that of the control group, and the difference between the groups is statistically significant (P<0.05), it means that after 12 hours of co-culture with saliva, the sample group can reduce the production of methyl mercaptan, and has the effect of refreshing breath and reducing bad breath.

[0150] 4. Test data and statistical results

[0151] Table 8 Halitosis removal test results

[0152]

[0153]

[0154] 5. Conclusion

[0155] Compared with the control group, the sample group was able to reduce the production of methyl mercaptan after 12 hours of co-culture with saliva. It can be considered that the fire-clearing toothpaste of the present invention has the effect of refreshing breath and reducing bad breath.

[0156] Test Example 4

[0157] This test example discloses the anti-inflammatory animal test of the fire-clearing toothpaste of the present invention. The details are as follows:

[0158] 1. Materials and Methods

[0159] 1. Test substance: the fire-clearing toothpaste of the present invention.

[0160] 2. Experimental animals and breeding environment

[0161] 24 SPF KM mice, male, 18g-22g, provided by Zhuhai Baishitong Biotechnology Co., Ltd., laboratory animal production license number: SCXK (Guangdong) 2020-0051, quality certificate number: No. 44822700025838. Feed was provided by Jiangsu Medison Biomedicine Co., Ltd. After purchase, the animals were raised in a barrier environment animal laboratory and used after passing the quarantine. Laboratory animal use license number: SYXK (Guangdong) 2018-0137. The temperature of the animal room was 20℃-26℃, and the humidity was 40%-70%.

[0162] 3. Instruments and Reagents

[0163] Main instruments: Sartorius electronic balance, model: QUINTIX224-1CN; 8mm hole puncher.

[0164] Reagents: Xylene.

[0165] 4. Test methods:

[0166] 4.1 The 24 SPF KM mice that passed the quarantine were randomly divided into two groups, with 12 mice in each group, namely the test sample group and the model control group.

[0167] 4.2 After the mice were quarantined and grouped, the left ear was not treated as the background value, and 30 μL of xylene solution was applied to the inner and outer surfaces of the right ear. After 30 minutes, the model control group was not treated, and 100 mg of the test substance was applied to both sides of the right ear of the mice in the test substance group. The mice were killed by cervical dislocation 1 hour after the application, the test substance was wiped off, the ears were cut off, and the ear pieces with a diameter of 8 mm (the same part of the left and right ears) were weighed and recorded. The weight difference between the left and right ears of the same animal is the swelling degree.

[0168] Swelling degree = weight of right ear piece - weight of left ear piece

[0169]

[0170] 4.3 Statistical analysis, experimental data are expressed as mean ± standard deviation SPSS software was used to perform independent sample T test.

[0171] 2. Test results

[0172] Compared with the model control group, the ear swelling of mice in the test group was reduced, and the difference was statistically significant (P<0.01). The inhibitory effect on mouse ear swelling is shown in the table below.

[0173] Table 9 Inhibitory effect of mouse ear swelling ( n=l2)

[0174] Group Ear swelling (mg) Ear swelling inhibition rate (%) Model control group 21.8±2.0 -- Test sample group 12.9±1.3** 41.08

[0175] Note: Compared with the model control group, **P<0.01.

[0176] Conclusion

[0177] Under the experimental conditions, the ear swelling of mice in the test group was lower than that in the model control group (P<0.01), indicating that the test sample has an anti-inflammatory effect in vivo.

[0178] Test Example 5

[0179] This test example discloses an in vitro evaluation test of the anti-plaque effect of the fire-clearing toothpaste of the present invention. The details are as follows:

[0180] 1. Experimental Grouping

[0181] Sample group: the fire-clearing toothpaste of the present invention

[0182] Negative control: deionized water

[0183] Control group: 0.1% chlorhexidine solution

[0184] 2. Experimental Methods

[0185] 1) Solution preparation

[0186] Sugar saliva medium: weigh 0.084 g of sucrose and add it to 84 g of saliva, and stir evenly.

[0187] Plaque growth medium: 6 g of pancreatic casein soy meat, 20 g of sucrose, and 2 g of gastric mucin were dissolved in 160.8 g of deionized water that had been sterilized by high-pressure steam for 15 min and cooled.

[0188] Glycolysis medium: Dissolve 6 g of tryptic soy milk and 10 g of sucrose in deionized water that has been autoclaved for 15 min and cooled, and make up to 200 mL.

[0189] Chlorhexidine solution (0.1% w / w): weigh 0.2 g of chlorhexidine and dissolve it in 200 mL of deionized water, stirring until fully dissolved.

[0190] Saliva collection: Collect 50 g of fresh human saliva every day, mix thoroughly, and refrigerate at 4°C for later use.

[0191] 2) Sample preparation: If the sample is toothpaste, mix the toothpaste and water in a mass ratio of 1:3 to form a uniform slurry; if the sample is mouthwash, use the original solution or dilute it according to the instructions.

[0192] 3) First day treatment: Add 7 mL of sugar saliva medium to each test tube, place a polished dry glass rod into a rod holder, immerse the other end of the glass rod in the sugar saliva medium for 1.5 cm, set the glass rod speed to 1 r / min, set the incubator temperature to 37°C, and culture overnight.

[0193] 4) Treatment on the second day: Take out the glass rod from the first day of culture and wash the test tube. Add 13.2 g of freshly collected whole saliva to the sterilized plaque growth medium and stir evenly. Add 15 mL of the plaque growth medium containing saliva to each test tube. Immerse the glass rod in the plaque growth medium containing saliva, culture at a constant temperature of 37°C for 6 hours, then take out the glass rod and immerse it in freshly collected saliva overnight.

[0194] 5) Treatment on the third day: Prepare 9 test tubes, add 15mL of the toothpaste slurry of the sample to be tested to the sample group, add 15mL of 0.1% chlorhexidine solution to the positive control group, and add 15mL of deionized water to the control group; prepare two rows of test tubes (9 tubes in each row), and add 10mL of deionized water to each. Take out the glass rods cultured on the second day, soak them in the toothpaste slurry for 60 times (1 second / time), then dip the glass rods in deionized water 10 times (1 second / time), and then dip them in clean deionized water 10 times (1 second / time). Prepare 9 test tubes, add 15mL of glycolysis medium to each, immerse the glass rods in the glycolysis medium and culture them at a constant temperature of 37°C for 6 hours, then take out the glass rods, and measure the pH value of the glycolysis medium in each test tube (keep 2 decimal places).

[0195] 3. Calculation and evaluation

[0196] Calculate the average pH value of each experimental group (retain 2 decimal places, relative standard deviation between parallels <15%), and calculate the plaque inhibition rate PI (retain 1 decimal place):

[0197]

[0198] Plaque inhibition rate PI ≥ 50% indicates effectiveness.

[0199] 4. Test data and statistical results

[0200] Table 10 Results of the dental plaque inhibition test

[0201]

[0202] 5. Conclusion

[0203] The fire-clearing toothpaste of the present invention can resist acid production caused by sugar metabolism and has the effect of inhibiting dental plaque.

[0204] The above embodiment is only one of the preferred implementation modes of the present invention and should not be used to limit the protection scope of the present invention. Any changes or modifications that are made to the main design concept and spirit of the present invention and have no substantive significance, and the technical problems they solve are still consistent with the present invention, should be included in the protection scope of the present invention.

Claims

1. A composition for removing heat and clearing internal heat, characterized in that: The invention comprises the following components in parts by weight: 0.1-0.5 parts of mugwort oil, 0.1-0.5 parts of mugwort slices, 0.1-0.5 parts of peppermint oil, 0.05-0.2 parts of eugenol, 0.1-0.5 parts of menthol and 0.1-0.5 parts of essence.

2. A composition for removing heat and clearing internal heat, characterized in that: The invention comprises the following components in parts by weight: 0.2 parts of mugwort oil, 0.2 parts of mugwort slices, 0.2 parts of peppermint oil, 0.1 parts of eugenol, 0.2 parts of menthol and 0.2 parts of essence.

3. The composition for removing heat and clearing fire according to claim 1, characterized in that: The Blumea balsamifera oil is the volatile oil of Blumea balsamifera, which is the leaves and young branches of the Blumea balsamifera plant of the Asteraceae family. The mint oil is volatile oil extracted from fresh stems and leaves of Mentha haplocalyx Briq., a plant of the Labiatae family, through steam distillation, freezing and partial brain removal.

4. Use of the composition according to any one of claims 1 to 3 in the preparation of oral products.

5. The use according to claim 4, characterized in that: Application in the preparation of oral products for inhibiting dental plaque and / or removing bad breath and / or removing internal heat and / or resisting inflammation and / or relieving pain.

6. Use according to claim 4 or 5, characterized in that The oral product comprises a paste, preferably a toothpaste.

7. A toothpaste comprising the composition according to any one of claims 1 to 3, characterized in that: Also includes 20-30 parts of friction agent Moisturizer 26-40 parts Thickener 0.6-2 parts 1.6-4 parts of surfactant; Flavoring agent 0.1-0.3 parts; Preservative 0.1-0.4 parts; pH regulator 0.01-0.5 parts; 25-35 parts of water.

8. The fire-clearing toothpaste according to claim 7, characterized in that: The friction agent comprises silica, preferably MII type silica or / and H silica; The moisturizing agent includes at least one of sorbitol and propylene glycol; The thickener comprises at least one of sodium carboxymethyl cellulose and / or xanthan gum; The surfactant includes at least one of sodium lauryl sulfate, cocamidopropyl betaine, and PEG-40 hydrogenated castor oil; The flavoring agent includes sodium saccharin; The preservatives include sodium benzoate; The pH adjuster is an alkaline substance, preferably sodium bicarbonate.

9. The method for preparing toothpaste according to claim 7 or 8, characterized in that: The following steps are involved: Weigh each component in proportion and mix them evenly.

10. The preparation method according to claim 8, characterized in that: The following steps are involved: Step 1. Prepare an effective component liquid: mix moxa slices, menthol, and part of the surfactant evenly, then add eugenol, Blumea balsamifera, peppermint oil and essence to the mixture, mix evenly, and obtain an effective component liquid; preferably, the surfactant in step 1 is PEG-40 hydrogenated castor oil; Step 2. Prepare a liquid material: mix water, a moisturizer, a preservative, a flavoring agent, and a pH adjuster, stir until dissolved, and then mix with the liquid surfactant in the remaining surfactant to obtain a liquid material; preferably, the liquid surfactant in step 2 is cocamidopropyl betaine; Step 3. Prepare powder: mix the friction agent, thickener and solid surfactant evenly to obtain powder; preferably, the solid surfactant in step 3 is sodium dodecyl sulfate; Step 4: After the liquid material and the powder material are evenly mixed, they are evenly mixed with the functional component liquid to obtain the toothpaste.

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