Liquid handling chip

Abstract

The present invention discloses a liquid processing chip. The front side of the chip body is provided with a sample area, a supernatant area group, a sedimentation liquid area group and a second capillary flow channel. The sample area, the supernatant area group, the sedimentation liquid area group and the second capillary flow channel are arranged in sequence from top to bottom, and the liquid in the sample area is separated in sequence from top to bottom. This facilitates multiple separations of the liquid in the sample area and the separation in sequence from top to bottom, thereby achieving vertical separation and making full use of the vertical space of the chip body. This facilitates the arrangement of the liquid processing chip along the circumference of a centrifuge. At the same time, the second capillary flow channel on the front side of the chip body and the first capillary flow channel on the back side of the chip body are interconnected, so as to fully utilize the front and back sides of the chip body, and the first output end and the second output end are both located on the front side of the chip body, so as to fully utilize the front side of the chip body.

Description

Technical Field

[0001] The present invention relates to the technical field of liquid processing chips, and in particular to a liquid processing chip. Background Art

[0002] With the development of science and technology, liquid processing chips are used to hold liquids and are used in conjunction with centrifuges. The liquid processing chip is clamped in the centrifuge and the liquid in the liquid processing chip is separated multiple times as the centrifuge rotates. In the prior art, the sample area of ​​the liquid processing chip holds liquid, and the liquid processing chip is also provided with other separation areas, which are the supernatant separation area and the precipitate separation area. The supernatant separation area and the precipitate separation area are arranged in a horizontal direction and are set to the right side of the sample area. At this time, the sample area, the supernatant separation area and the precipitate separation area utilize the horizontal space of the chip body, resulting in the inability to achieve vertical separation of the liquid. Summary of the Invention

[0003] The object of the present invention is to overcome the shortcomings of the prior art. The present invention provides a liquid processing chip, in which the liquid in the sample area is separated sequentially along the top-down direction; so that the liquid in the sample area is separated multiple times and separated sequentially along the top-down direction, thereby realizing vertical separation, making full use of the vertical space of the chip body, so that the liquid processing chip is arranged along the circumference of the centrifuge, and at the same time, the second capillary flow channel on the front side of the chip body and the first capillary flow channel on the back side of the chip body are interconnected, so as to make full use of the front and back sides of the chip body, and the first output end and the second output end are both located on the front side of the chip body, so as to make full use of the front side of the chip body, thereby improving the space utilization of the liquid processing chip.

[0004] In order to solve the above technical problems, an embodiment of the present invention provides a liquid processing chip, comprising:

[0005] The front of the chip body is provided with a sample area, a supernatant area group, a sedimentation area group, and a second capillary flow channel; the sample area, the supernatant area group, the sedimentation area group, and the second capillary flow channel are arranged in sequence from top to bottom, and the liquid in the sample area is separated in sequence from top to bottom;

[0006] A mixing area and a first capillary channel are provided on the reverse side of the chip body; the mixing area is connected to the end of the precipitate separation area and accommodates the resuspension; the resuspension flows from the mixing area to the first capillary channel, wherein the first capillary channel is connected to the second capillary channel and output to the first output end on the front side of the chip body, and the end of the supernatant area group is output to the second output end on the front side of the chip body.

[0007] Optionally, the sample area is provided at the top of the front surface of the chip body: the sample area is used to contain liquid;

[0008] The supernatant liquid region group includes a supernatant liquid separation region; the precipitate liquid region group includes a precipitate liquid separation region;

[0009] The sample area, the supernatant separation area and the precipitate separation area are sequentially connected in a vertical downward direction, and the liquid in the sample area is separated into a supernatant and a precipitate in a first separation; the supernatant is in the supernatant separation area; and the precipitate is in the precipitate separation area.

[0010] Optionally, the supernatant area group further includes a supernatant quantitative area and a supernatant siphon flow channel, and the supernatant quantitative area is located below the supernatant separation area;

[0011] The supernatant siphon flow channel is located between the supernatant separation area and the supernatant quantitative area; when the first siphon valve in the supernatant siphon flow channel is started, the supernatant in the supernatant quantitative area is sequentially introduced into the supernatant quantitative area.

[0012] Optionally, the supernatant area group also includes a supernatant output flow channel, which is located below the supernatant quantitative area; the supernatant output flow channel is located between the supernatant quantitative area and the second output end on the front side of the chip body, and connects the supernatant quantitative area and the second output end on the front side of the chip body.

[0013] Optionally, the sedimentation liquid area group also includes a sedimentation liquid cache area and a sedimentation liquid siphon flow channel; the sedimentation liquid cache area is located below the sedimentation liquid separation area; the sedimentation liquid siphon flow channel is located between the sedimentation liquid separation area and the sedimentation liquid cache area; when the second siphon valve in the sedimentation liquid siphon flow channel is started, the sedimentation liquid in the sedimentation liquid separation area is sequentially introduced into the sedimentation liquid cache area.

[0014] Optionally, a precipitate quantitative area is provided on the reverse side of the chip body, and the precipitate quantitative area is connected to the precipitate separation area or the precipitate buffer area;

[0015] The mixing area is located below the precipitate quantitative area and is connected to the precipitate quantitative area.

[0016] Optionally, the mixing area is connected to the first capillary flow channel, the first capillary flow channel and the mixing area are arranged flush with each other in a horizontal direction, and the first capillary flow channel is located on the left side of the mixing area.

[0017] Optionally, the first capillary flow channel is a serpentine flow channel; one end of the first capillary flow channel is connected to the mixing area, and the other end is connected to one end of the second capillary flow channel, and the other end of the second capillary flow channel is connected to the second output end on the front side of the chip body.

[0018] Optionally, there are multiple second capillary flow channels, and the multiple second capillary flow channels are arranged side by side in the horizontal direction and connected to one end of the same first capillary flow channel, and the other ends of the multiple second capillary flow channels are all connected to the first output end on the front side of the chip body.

[0019] Optionally, the second capillary flow channel is a serpentine flow channel and extends in a downward direction.

[0020] In an embodiment of the present invention, through the liquid processing chip in the embodiment of the present invention, the liquid in the sample area is separated in sequence along the top-down direction; so that the liquid in the sample area is separated multiple times, and is separated in sequence along the top-down direction, thereby realizing vertical separation, making full use of the vertical space of the chip body, so that the liquid processing chip is arranged along the circumference of the centrifuge, and at the same time, the second capillary flow channel on the front side of the chip body and the first capillary flow channel on the back side of the chip body are connected to each other, so as to make full use of the front and back sides of the chip body, and the first output end and the second output end are both on the front side of the chip body, so as to make full use of the front side of the chip body, thereby improving the space utilization of the liquid processing chip. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0022] Figure 1 is a schematic diagram of a blood processing chip in an embodiment of the present invention;

[0023] Figure 2 is a front schematic diagram of a blood processing chip in an embodiment of the present invention;

[0024] Figure 3 is a schematic diagram of the reverse side of the blood processing chip in an embodiment of the present invention;

[0025] Figures 4 to 8 Schematic diagram of multiple separations of the liquid processing chip in an embodiment of the present invention.

[0026] Reference numerals:

[0027] Liquid processing chip 100, front surface 10 of chip body, back surface 20 of chip body, mixing area 21, first capillary channel 22;

[0028] Sample area 11, supernatant area group 12, sedimentation liquid area group 13, second capillary channel 14, second output end 15, first output end 16;

[0029] The supernatant separation area 121 , the supernatant quantitative area 122 , the supernatant siphon flow channel 123 , the supernatant output flow channel 124 , the precipitate separation area 131 , the precipitate buffer area 132 , the precipitate siphon flow channel 133 , and the precipitate quantitative area 134 . DETAILED DESCRIPTION

[0030] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0031] See also Figures 1 to 8 The liquid processing chip 100 of the present application is used to contain liquid and is used in conjunction with a centrifuge. The liquid processing chip 100 is clamped in the centrifuge and performs multiple separations on the liquid in the liquid processing chip 100 as the centrifuge rotates. The liquid processing chip 100 includes a front side 10 of the chip body and a back side 20 of the chip body, and the front side 10 of the chip body is provided with a sample area 11, a supernatant area group 12, a precipitate area group 13 and a second capillary channel 14; the back side 20 of the chip body is provided with a mixing area 21 and a first capillary channel 22.

[0032] The liquid processing chip 100 concentrates more areas on the front side 10 of the chip body and makes full use of the vertical space of the front side 10 of the chip body. In the front side 10 of the chip body, the sample area 11, the supernatant area group 12, the sedimentation liquid area group 13 and the second capillary channel 14 are arranged in sequence from top to bottom, and the liquid in the sample area 11 is separated in sequence from top to bottom.

[0033] Among them, the liquid in the sample area 11 is separated in sequence from top to bottom; so that the liquid in the sample area 11 is separated multiple times and separated in sequence from top to bottom, thereby realizing vertical separation and making full use of the vertical space of the chip body, so that the liquid processing chip 100 is arranged along the circumference of the centrifuge.

[0034] The sample area 11 is located at the top of the front surface 10 of the chip body. The sample area 11 is used to hold liquid, so that the liquid is stored on the front surface 10 of the chip body through the sample area 11. The sample area 11 also utilizes the top space of the front surface 10 of the chip body to facilitate vertical separation of the liquid. Optionally, the sample area 11 is a special-shaped area and is connected to the air vent.

[0035] Relative to the supernatant area group 12, the supernatant area group 12 includes a supernatant separation area 121, a supernatant quantification area 122, a supernatant siphon flow channel 123 and a supernatant output flow channel 124. The supernatant separation area 121, the supernatant siphon flow channel 123, the supernatant quantification area 122 and the supernatant output flow channel 124 are arranged in sequence along the vertical downward direction and are connected in sequence along the vertical downward direction, so as to facilitate the liquid in the sample area 11 to flow downward along the supernatant separation area 121, the supernatant siphon flow channel 123, the supernatant quantification area 122 and the supernatant output flow channel 124 in sequence and be gradually separated.

[0036] At the same time, the sedimentation liquid area group 13 is arranged below the supernatant liquid area group 12. At this time, the sedimentation liquid area group 13 includes a sedimentation liquid separation area 131, a sedimentation liquid buffer area 132 and a sedimentation liquid siphon channel 133. At this time, the sample area 11, the supernatant liquid separation area 121 and the sedimentation liquid separation area 131 are connected in sequence along the vertical downward direction, and the liquid in the sample area 11 is separated into supernatant and sedimentation liquid in the first separation; the supernatant is in the supernatant separation area 121; the sedimentation liquid is in the sedimentation liquid separation area 131.

[0037] Therefore, the precipitate separation area 131 is located below the supernatant separation area 121 and fully utilizes the space below the supernatant separation area 121. The liquid in the sample area 11 is separated into supernatant and precipitate in the first separation. At this time, the supernatant is in the supernatant separation area 121 and the precipitate is in the precipitate separation area 131.

[0038] The supernatant quantitative area 122 is located below the supernatant separation area 121, and the supernatant is further processed in the first separation of the liquid. At this time, the supernatant siphon channel 123 is located between the supernatant separation area 121 and the supernatant quantitative area 122; when the first siphon valve in the supernatant siphon channel 123 is started, the supernatant in the supernatant quantitative area 122 is sequentially introduced into the supernatant quantitative area 122.

[0039] Among them, the supernatant gradually accumulates in the supernatant siphon flow channel 123, and when the supernatant siphon flow channel 123 is filled, the first siphon valve in the supernatant siphon flow channel 123 is triggered, and the first siphon valve in the supernatant siphon flow channel 123 is adjusted from a closed state to a started state. In the started state, the first siphon valve in the supernatant siphon flow channel 123 allows the supernatant in the supernatant quantitative area 122 to enter the supernatant quantitative area 122 in sequence, so as to ensure that the supernatant in the supernatant quantitative area 122 enters the supernatant quantitative area 122, and in the vertical separation process, the supernatant's own gravity is fully utilized, the use of the pump body is reduced, and the separation is more sufficient.

[0040] Regarding the supernatant output flow channel 124, the supernatant output flow channel 124 is located below the supernatant quantitative area 122; the supernatant output flow channel 124 is located between the supernatant quantitative area 122 and the second output end 15 of the front side 10 of the chip body, and connects the supernatant quantitative area 122 with the second output end 15 of the front side 10 of the chip body, so as to complete the processing of the supernatant, and the processing of the supernatant is concentrated on the front side 10 of the chip body, so as to facilitate the centralized processing of the liquid separation and the gradual separation of the supernatant, making full use of the vertical separation characteristics and the vertical space of the front side 10 of the chip body, thereby improving the space utilization rate of the front side 10 of the chip body.

[0041] In an embodiment of the present application, the back surface 20 of the chip body is arranged on the opposite side of the front surface 10 of the chip body. At this time, the back surface 20 of the chip body is provided with a mixing area 21 and a first capillary channel 22; the mixing area 21 is connected to the end of the precipitate separation area 131 and accommodates the resuspension; the resuspension flows from the mixing area 21 to the first capillary channel 22, wherein the first capillary channel 22 is connected to the second capillary channel 14 and output to the first output end 16 of the front surface 10 of the chip body, and the end of the supernatant area group 12 is output to the second output end 15 of the front surface 10 of the chip body.

[0042] Among them, the second capillary channel 14 on the front side 10 of the chip body and the first capillary channel 22 on the back side 20 of the chip body are connected to each other, so as to fully utilize the front side 10 and the back side of the chip body, and the first output end 16 and the second output end 15 are both located on the front side 10 of the chip body, so as to fully utilize the front side 10 of the chip body, thereby improving the space utilization of the liquid processing chip 100.

[0043] At this time, the second capillary channel 14 is on the front side 10 of the chip body, the first output end 16 is also on the front side 10 of the chip body, and the back side 20 of the chip body is fully utilized, the mixing area 21 and the first capillary channel 22 are set on the back side 20 of the chip body, and the mixing area 21 is connected to the sedimentation liquid buffer area 132 and the sedimentation liquid siphon channel 133 on the front side 10 of the chip body. At the same time, the connection between the first capillary channel 22 and the second capillary channel 14 is utilized to realize the transfer of the heavy suspension on the back side 20 of the chip body to the front side 10 of the chip body, and the connection function between the first capillary channel 22 and the second capillary channel 14 is fully utilized.

[0044] In addition, the sedimentation liquid area group 13 also includes a sedimentation liquid buffer area 132 and a sedimentation liquid siphon channel 133; the sedimentation liquid buffer area 132 is located below the sedimentation liquid separation area 131; the sedimentation liquid siphon channel 133 is located between the sedimentation liquid separation area 131 and the sedimentation liquid buffer area 132; when the second siphon valve in the sedimentation liquid siphon channel 133 is started, the sedimentation liquid in the sedimentation liquid separation area 131 is sequentially introduced into the sedimentation liquid buffer area 132 to facilitate further separation of the sedimentation liquid.

[0045] Optionally, a precipitate quantification area 134 is provided on the reverse side 20 of the chip body, and the precipitate quantification area 134 is connected to the precipitate separation area 131 or the precipitate buffer area 132; the mixing area 21 is below the precipitate quantification area 134 and is connected to the precipitate quantification area 134, so that the precipitate and the reagent are mixed in the mixing area 21.

[0046] In another embodiment of the present application, the mixing region 21 is connected to the first capillary channel 22 , and the first capillary channel 22 is arranged flush with the mixing region 21 in the horizontal direction. The first capillary channel 22 is located on the left side of the mixing region 21 .

[0047] Optionally, the first capillary channel 22 is a serpentine channel; one end of the first capillary channel 22 is connected to the mixing region 21, and the other end is connected to one end of the second capillary channel 14. The other end of the second capillary channel 14 is connected to the first output port 16 of the front surface 10 of the chip body. There are multiple second capillary channels 14, each arranged side by side horizontally and connected to one end of the same first capillary channel 22. The other ends of each of the second capillary channels 14 are connected to the first output port 16 of the front surface 10 of the chip body. Optionally, the second capillary channel 14 is a serpentine channel and extends downward.

[0048] In an embodiment of the present invention, through the liquid processing chip 100 in the embodiment of the present invention, the liquid in the sample area 11 is separated in sequence along the top-down direction; so that the liquid in the sample area 11 is separated multiple times and separated in sequence along the top-down direction, thereby realizing vertical separation, making full use of the vertical space of the chip body, so that the liquid processing chip 100 is arranged along the circumference of the centrifuge, and at the same time, the second capillary flow channel 14 of the front side 10 of the chip body and the first capillary flow channel 22 of the back side 20 of the chip body are connected to each other, so as to make full use of the front side 10 and the back side of the chip body, and the first output end 16 and the second output end 15 are both located on the front side 10 of the chip body, so as to make full use of the front side 10 of the chip body, thereby improving the space utilization of the liquid processing chip 100.

[0049] In addition, the liquid processing chip provided in the embodiment of the present invention is introduced in detail above. Specific examples are used herein to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the method of the present invention and its core idea. At the same time, for those skilled in the art, according to the idea of ​​the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A liquid processing chip, characterized in that: include: The front of the chip body is provided with a sample area, a supernatant area group, a sedimentation area group and a second capillary flow channel; The sample area, the supernatant liquid area group, the sedimentation liquid area group and the second capillary flow channel are sequentially arranged in a top-to-bottom direction, and the liquid in the sample area is sequentially separated in a top-to-bottom direction; The back side of the chip body is provided with a mixing area and a first capillary flow channel; the mixing area is connected to the end of the precipitate separation area and contains the resuspension; the resuspension flows from the mixing area to the first capillary flow channel, wherein the first capillary flow channel is connected to the second capillary flow channel and output to the first output end of the front side of the chip body, and the end of the supernatant area group is output to the second output end of the front side of the chip body; The sample area is arranged at the top of the front surface of the chip body; the sample area is used to accommodate liquid; the supernatant area group includes a supernatant separation area; the precipitate area group includes a precipitate separation area; the sample area, the supernatant separation area and the precipitate separation area are sequentially connected in a vertical downward direction, and the liquid in the sample area is separated into a supernatant and a precipitate in a first separation; the supernatant is in the supernatant separation area; the precipitate is in the precipitate separation area; The supernatant area group further includes a supernatant quantitative area and a supernatant siphon flow channel, and the supernatant quantitative area is located below the supernatant separation area; The supernatant siphon flow channel is located between the supernatant separation area and the supernatant quantitative area; when the first siphon valve in the supernatant siphon flow channel is activated, the supernatant in the supernatant quantitative area is sequentially drawn into the supernatant quantitative area; The sedimentation liquid area group also includes a sedimentation liquid cache area and a sedimentation liquid siphon flow channel; the sedimentation liquid cache area is located below the sedimentation liquid separation area; the sedimentation liquid siphon flow channel is located between the sedimentation liquid separation area and the sedimentation liquid cache area; when the second siphon valve in the sedimentation liquid siphon flow channel is started, the sedimentation liquid in the sedimentation liquid separation area will enter the sedimentation liquid cache area in sequence.

2. The liquid processing chip according to claim 1, characterized in that The supernatant area group also includes a supernatant output flow channel, which is located below the supernatant quantitative area; the supernatant output flow channel is located between the supernatant quantitative area and the second output end on the front side of the chip body, and connects the supernatant quantitative area and the second output end on the front side of the chip body.

3. The liquid processing chip according to claim 1, characterized in that A precipitate quantitative area is provided on the reverse side of the chip body, and the precipitate quantitative area is connected to the precipitate separation area or the precipitate buffer area; The mixing area is located below the precipitate quantitative area and is connected to the precipitate quantitative area.

4. The liquid processing chip according to claim 3, characterized in that The mixing area is connected to the first capillary flow channel. The first capillary flow channel is arranged flush with the mixing area in a horizontal direction. The first capillary flow channel is located on the left side of the mixing area.

5. The liquid processing chip according to claim 4, characterized in that: The first capillary flow channel is a serpentine flow channel; one end of the first capillary flow channel is connected to the mixing area, and the other end is connected to one end of the second capillary flow channel, and the other end of the second capillary flow channel is connected to the second output end on the front side of the chip body.

6. The liquid processing chip according to claim 5, characterized in that: There are multiple second capillary flow channels, which are arranged side by side in the horizontal direction and connected to one end of the same first capillary flow channel. The other ends of the multiple second capillary flow channels are all connected to the first output end on the front side of the chip body.

7. The liquid processing chip according to claim 5, characterized in that: The second capillary flow channel is a serpentine flow channel and extends in a downward direction.

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