Pipetting module and nucleic acid detector

Abstract

The invention discloses a pipetting module which comprises a pipetting supporting frame, at least one pipetting unit is installed on the pipetting supporting frame, each pipetting unit comprises a horizontal rail, a horizontal sliding block is installed on the horizontal rail, a tip head replacement assembly is installed on the horizontal sliding block, and the tip head replacement assembly comprises a rail base fixedly installed on the horizontal sliding block. A moving seat is arranged on the track seat, the moving seat can move in the vertical direction relative to the track seat, a connecting gun is installed on the moving seat, and a connecting gun head used for being matched with a tip head is arranged at the lower end of the connecting gun; a tip head taking and placing control assembly is arranged between the connecting gun and the movable base. The tip head taking and placing control assembly comprises a gun head sleeve, a telescopic rod and a taking and placing control mechanism. The gun head sleeve is arranged outside the connecting gun in a sleeving mode and used for being matched with the tip head in a limiting mode. The pick-and-place control mechanism is used for guiding the gun head sleeve to move; a limiting groove is formed in the gun head sleeve, and the telescopic rod is installed on the rail base and can stretch into the limiting groove. The invention also discloses a nucleic acid detector.

Description

Technical Field

[0001] The present invention belongs to the technical field of medical devices, and specifically relates to a liquid transfer module and a nucleic acid detector. Background Art

[0002] Nucleic acid extraction and PCR (Polymerase Chain Reaction) amplification technology are widely used in the field of molecular diagnosis. The main principle is to extract nucleic acids from the sample to be tested and amplify the extracted nucleic acids. In early experiments, nucleic acid extraction and PCR amplification were both done manually, which was time-consuming and had poor consistency in experimental results. With the development of random industrial automation, the emergence of nucleic acid extractors and quantitative PCR instruments has replaced and upgraded the manual work in the nucleic acid extraction and PCR amplification processes. However, the process of adding samples to the nucleic acid extractor and loading the extracted samples into the quantitative PCR instrument still requires manual operation and transfer, which not only increases the workload of the staff, but also makes the samples easily contaminated during the transfer process. During the nucleic acid extraction and PCR amplification process, it is necessary to pipette samples and different reagents. Different pipetting operations require the use of different tip heads. Therefore, how to automatically replace the tip during nucleic acid extraction and PCR amplification has become an important problem that restricts the automation of nucleic acid detection. Summary of the invention

[0003] In view of this, an object of the present invention is to provide a pipetting module and a nucleic acid detector that can automatically load and unload tips to meet the requirements of pipetting during nucleic acid extraction and PCR amplification.

[0004] In order to achieve the above object, the present invention provides the following technical solutions:

[0005] The present invention first proposes a pipetting module, comprising a pipetting support frame, wherein at least one pipetting unit is installed on the pipetting support frame, wherein the pipetting unit comprises a horizontal track, wherein a horizontal slider slidably matched with the horizontal track is installed on the horizontal track, wherein a tip head replacement assembly is installed on the horizontal slider, wherein the tip head replacement assembly comprises a track seat fixedly installed on the horizontal slider, wherein a movable seat is arranged on the track seat, wherein the movable seat can move in a vertical direction relative to the track seat, wherein a connecting gun is installed on the movable seat, wherein the connecting gun is located in a vertical direction, and wherein a connecting gun head for matching with a tip head is arranged at the lower end of the connecting gun;

[0006] A tip head pick-and-place control assembly is provided between the connecting gun and the movable seat; the tip head pick-and-place control assembly comprises a gun head cover, a telescopic rod and a pick-and-place control mechanism, the gun head cover is provided outside the connecting gun and is used to cooperate with the tip head in a limiting manner; the pick-and-place control mechanism is used to guide the gun head cover to move in a vertical direction, and the lowest position of the gun head cover moving downward relative to the connecting gun reaches the connecting gun head or reaches below the connecting gun head; a limiting groove is provided on the gun head cover, the telescopic rod is installed on the track seat, and the telescopic rod can be extended into the limiting groove.

[0007] Furthermore, the connecting gun head is provided with at least one convex ring for interference fit with the tip head.

[0008] Furthermore, a mounting through hole is provided in the movable seat, and the connecting gun is fixedly installed in the mounting through hole.

[0009] Furthermore, the pick-and-place control mechanism includes a guide rod parallel to the connecting gun, and the gun head cover moves synchronously with the guide rod; the movable seat is provided with a guide through hole that slides with the guide rod, and the upper end of the guide rod is provided with a limiting structure that limits the movable seat.

[0010] Furthermore, a compression spring for applying a downward elastic force to the guide rod is provided between the lower end of the guide rod and the movable seat.

[0011] Furthermore, a small-diameter guide rod section is provided at the upper end of the guide rod, and a large-diameter guide rod section is provided at the lower end, and the outer diameter of the large-diameter guide rod section is larger than the outer diameter of the small-diameter guide rod section; the compression spring is installed between the moving seat and the large-diameter guide rod section; a large-diameter guide hole section is provided at the lower end of the guide through hole, and a small-diameter guide hole section is provided at the upper end, and the inner diameter of the large-diameter guide hole section is larger than the inner diameter of the small-diameter guide hole section; the large-diameter guide hole section cooperates with the large-diameter guide rod section, and the small-diameter guide rod section cooperates with the small-diameter guide hole section and extends upward from the small-diameter guide hole section, the limiting structure is arranged on the small-diameter guide rod section, and the compression spring is installed in the large-diameter guide hole section.

[0012] Furthermore, a connecting seat is provided on the connecting sleeve, the guide rod is fixedly connected to the connecting seat, and the limiting groove is arranged in the connecting seat.

[0013] Furthermore, an optical coupling sensor for detecting the position of the moving seat is installed on the track seat, and a light blocking plate cooperating with the optical coupling sensor is installed on the moving seat.

[0014] Furthermore, a tube cap pressing plate is installed on the movable seat, and the tube cap pressing plate is used to press the tube cap loaded in the PCR tube during the PCR process.

[0015] Furthermore, the tube cap pressure plate includes a pressure cover plate located in the horizontal direction, and connecting plates are respectively provided at both ends of the pressure cover plate, and the connecting plates are fixedly connected to the movable seat; the distance between the geometric center of the pressure cover plate and the axis of the connecting gun is equal to the spacing between the sample storage tube and the PCR tube in the nucleic acid detection card box.

[0016] Furthermore, a central through hole is provided in the connecting gun; and the pipetting unit also includes a pipetting pipeline system connected to the central through hole.

[0017] Furthermore, the pipette pipeline system includes a plunger pump and a three-way solenoid valve. Among the three connection ports of the three-way solenoid valve, the first connection port is connected to the plunger pump, the second connection port is provided with an air filter connected to the atmosphere, and a pipette connecting tube is provided between the third connection port and the central through hole, the pipette connecting tube is connected to an air pressure detection tube, and the air pressure detection tube is connected to an air pressure sensor.

[0018] The present invention also proposes a nucleic acid detection instrument, comprising a base plate, on which are mounted an in-and-out bin assembly and the pipetting module as described above, the in-and-out bin assembly comprising an in-and-out bin track located in a horizontal direction and perpendicular to the horizontal track, the in-and-out bin track being mounted with an in-and-out bin slider slidably matched therewith, the in-and-out bin slider being mounted with a base assembly; the base plate is provided with an in-and-out bin drive mechanism for driving the in-and-out bin slider to move along the in-and-out bin track between an exit position and an entry position.

[0019] The beneficial effects of the present invention are:

[0020] The pipetting module of the present invention is provided with a tip head replacement assembly on a horizontal slider, and a vertical track is provided on the track seat of the tip head replacement assembly, and a connecting gun is installed on a movable seat that can move along the vertical track. In this way, the connecting gun can move along the horizontal track and the vertical track. Combined with the in-and-out cabin movement of the base assembly in the nucleic acid detector, the connecting gun can move in three directions perpendicular to each other relative to the base assembly to meet the movement requirements of pipetting.

[0021] By setting a tip head pick-and-place control component between the connecting gun and the moving seat, the tip head pick-and-place control component is used to load or unload the tip head. The principle is: a gun head cover is set on the connecting gun to limit the tip head, and the gun head cover is guided to move in the vertical direction by the pick-and-place control mechanism so that the lowest position of the gun head cover moving downward relative to the connecting gun reaches the connecting gun head or is located below the connecting gun head, so that the tip head loaded on the connecting gun head can be unloaded; at the same time, the limiting matching relationship between the telescopic rod and the limiting groove of the gun head cover is used to drive the gun head cover to move relative to the connecting gun; in this way, when the tip head needs to be unloaded, the telescopic rod is controlled to extend and extend into the limiting groove, driving the moving seat to move upward along the vertical track, and between the gun head cover and the tip head. Under the limit matching relationship, the gun head cover first moves upward with the connecting gun; when the telescopic rod is limitedly matched with the lower end of the limit groove, the gun head cover can no longer move upward. At this time, the connecting gun continues to be driven upward, and the gun head cover moves downward relative to the connecting gun, while overcoming the assembly force between the tip head and the connecting gun, so that the tip head is separated from the connecting gun head to achieve the purpose of unloading the tip; after the tip head is unloaded, the telescopic rod is controlled to retract out of the limit groove. At this time, the gun head cover continues to move downward relative to the connecting gun under the action of gravity until the guide rod is limitedly matched with the moving seat; to load the tip, the connecting gun is directly inserted into the corresponding tip head, and the tip head is loaded on the connecting gun by utilizing the interference fit relationship between the connecting gun head and the tip head. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to make the purpose, technical solution and beneficial effects of the present invention clearer, the present invention provides the following drawings for illustration:

[0023] Figure 1 This is a front view of the nucleic acid detection cartridge;

[0024] Figure 2 for Figure 1 AA section view;

[0025] Figure 3 This is an exploded view of the nucleic acid test cartridge;

[0026] Figure 4 This is an axonometric view of a nucleic acid detection cartridge;

[0027] Figure 5-6 This is an axonometric diagram of a nucleic acid detector;

[0028] Figure 7 is a side view of the base assembly;

[0029] Figure 8 is a front view of the base assembly;

[0030] Fig. 9 is a cross-sectional view of a base assembly;

[0031] Figure 10-12 A process diagram for unloading a card cartridge from a card cartridge mounting seat;

[0032] Fig.13 It is a structural schematic diagram of the entry and exit cabin assembly;

[0033] Fig.14 This is a schematic diagram of the structure of the cabin entry and exit slider when entering the cabin;

[0034] Fig.15 It is a schematic diagram of the structure of the entry and exit slider when it is in the exit position;

[0035] Figure 16-17 This is an axonometric diagram of the PCR temperature control component;

[0036] Fig.18 It is a structural schematic diagram of a PCR temperature control unit;

[0037] Fig.19 This is a position relationship diagram of the PCR temperature control unit and the card box when the PCR temperature control unit is in the avoidance position;

[0038] Fig. 20 It is a position relationship diagram of the PCR temperature control unit and the card box when it is in the temperature control position;

[0039] Fig.21 It is a schematic diagram of the position of the magnetic suction component when the PCR temperature control unit is in the magnetic suction position;

[0040] Fig. 22 It is a schematic diagram of the position of the magnetic suction component when the PCR temperature control unit is in the avoidance position;

[0041] Fig.23 It is a schematic diagram of the structure of the photovoltaic module;

[0042] Figure 24-25 It is an axonometric view of the pipetting module;

[0043] Fig.26 It is a schematic diagram of the structure of the liquid transfer unit;

[0044] Fig. 27 for Fig.26 A magnified view of area B;

[0045] Fig.28 The position relationship diagram of the cap pressing plate and the cartridge when loading or unloading the tip head for the connecting gun;

[0046] Fig.29 The diagram is a position relationship diagram of the tube cap pressing plate and the cartridge when the tip head is located in the sample storage tube;

[0047] Fig.30 This is a schematic diagram of the structure when the tube cap pressing plate presses the PCR tube cap during the PCR process.

[0048] Description of reference numerals:

[0049] 100-card box; 110-card box body; 111-nucleic acid extraction area; 112-PCR amplification area; 113-reagent loading area; 114-sample storage tube; 115-waste liquid storage tube; 116-proteinase K storage tube; 117-lysate storage tube; 118-paraffin oil storage tube; 119-dilution storage tube; 120-cleaning liquid storage tube; 121-elution storage tube; 122-first tip head; 123-second tip head; 124-first tip head storage tube; 125-second tip head storage tube; 1 26-PCR tube; 127-PCR tube cap; 128-tube cap loading slot; 129-PCR reagent tube; 130-internal standard reagent tube; 131-first mounting slot; 132-first mounting hole; 133-second mounting slot; 134-through hole; 135-through hole; 136-giving slot; 137-second mounting hole; 138-card box side plate; 139-giving hole; 140-clip plate; 141-clip clip; 142-guide slope; 143-anti-slip structure; 144-identification plate; 150-reagent plate; 151-clip;

[0050] 201-upper base plate; 202-lower base plate; 203-support column; 204-base space; 205-power supply assembly; 206-support frame; 207-display assembly;

[0051] 300-base module; 310-base assembly; 311-mounting base; 312-card box mounting seat; 313-temperature control element; 314-variable temperature metal; 315-hot well; 316-heat dissipation assembly; 317-temperature control chamber; 318-upper opening; 319-temperature control slot; 320-heat dissipation seat; 321-heat sink; 322-temperature control mounting seat; 323-RFID reader; 324-heat dissipation channel; 325-mounting through hole; 326-cooling fan; 327-pressing block; 328-mounting arm; 329-magnet; 330-magnetic guide rod; 331-magnetic slider; 332-magnetic reset spring; 333-magnetic lever; 334-fixed shaft; 335-horizontal slide groove; 336-matching shaft; 337-bottom plate; 338-base side plate; 339-card hole;

[0052] 350-cabin entry and exit assembly; 351-cabin entry and exit track; 352-cabin entry and exit slider; 353-base mounting plate; 354-cabin entry and exit drive motor; 355-synchronous pulley; 356-synchronous belt; 357-cabin door; 358-double-hinged connecting rod; 359-reinforcement plate; 360-bending section; 361-first roller; 362-second roller; 363-cabin door return spring; 364-bending connecting section;

[0053] 370-PCR temperature control assembly; 371-PCR temperature control unit; 372-PCR temperature control element; 373-PCR temperature-changing metal; 374-PCR hot well; 375-heat sink; 376-heat dissipation channel; 377-heat sink; 377a-cooling fan; 378-fixing plate; 379-lifting rail; 380-lifting slider; 381-lifting motor; 382-screw rod; 383-lifting seat; 384-driving arm; 385-driving wheel; 386-optical fiber positioning assembly;

[0054] 390-photoelectric module; 391-excitation optical fiber; 392-receiving optical fiber; 393-photoelectric mounting seat; 394-optical fiber connection board; 395-photoelectric detector; 396-photoelectric detection drive assembly;

[0055] 400-pipette module; 401-pipette support frame; 402-horizontal track seat; 403-horizontal track; 404-horizontal slider; 405-track seat; 406-vertical track; 407-moving seat; 408-connecting gun; 409-convex ring; 410-mounting through hole; 411-pressing connector; 412-gun head cover; 413-guide rod; 413a-small diameter guide rod section; 413b-large diameter guide rod section; 414-connecting seat; 415-guide through hole; 415a-large diameter guide hole section; 415b-small diameter guide hole section ;416-limiting structure;417-limiting groove;418-compression spring;419-first screw rod;420-first motor;421-second screw rod;422-second motor;423-optical coupling sensor;424-light shielding plate;425-tube cap pressure plate;425a-pressure cover plate;425b-connecting plate;426-center through hole;427-plunger pump;428-three-way solenoid valve;429-air filter;430-pipette connecting tube;431-air pressure detection tube;432-air pressure sensor;433-telescopic rod. DETAILED DESCRIPTION

[0056] The present invention is further described below in conjunction with the accompanying drawings and specific embodiments so that those skilled in the art can better understand the present invention and implement it, but the embodiments are not intended to limit the present invention.

[0057] like Figure 1 As shown, the cartridge 100 of this embodiment includes a cartridge body 110 and a reagent plate 150. The cartridge body 110 of this embodiment is provided with a nucleic acid extraction area 111, a PCR amplification area 112 and a reagent loading area 113. In this embodiment, the reagent loading area 113 is located between the nucleic acid extraction area 111 and the PCR amplification area 112.

[0058] A plurality of storage tubes are provided in the nucleic acid extraction area 111. Specifically, the storage tubes include a sample storage tube 114, a waste liquid storage tube 115, a proteinase K storage tube 116, a lysate storage tube 117, a paraffin oil storage tube 118, a diluent storage tube 119, a cleaning liquid storage tube 120, and an eluent storage tube 121. Among them, the sample storage tube 114 is used to store samples and perform nucleic acid extraction; the waste liquid storage tube 115 is used to store waste liquid generated during nucleic acid extraction; the proteinase K storage tube 116 is used to store proteinase K; the lysate storage tube 117 is used to store magnetic bead lysate; the paraffin oil storage tube 118 is used to store paraffin oil; the diluent storage tube 119 is used to store diluent, and the diluent in this embodiment uses water; the cleaning liquid storage tube 120 is used to store cleaning liquid; and the eluent storage tube 121 is used to store eluent. In a preferred implementation of this embodiment, the storage tube also includes a tip storage tube for placing a tip. Specifically, the tip head includes a first tip head 122 for use in the nucleic acid extraction process and a second tip head 123 for use in the PCR amplification process. In this embodiment, the capacity of the first tip head 122 to transfer liquid each time is 500ul, and the capacity of the second tip head 123 to transfer liquid each time is 100ul. A tip head is placed in each tip head storage tube, that is, the tip head storage tube of this embodiment includes a first tip head storage tube 124 for placing the first tip head 122 and a second tip head storage tube 125 for placing the second tip head 123. Specifically, in this embodiment, the second tip head 123 is set in a one-to-one correspondence with the PCR tube 126.

[0059] The PCR amplification area 112 is provided with PCR tubes 126 for PCR amplification. In the present embodiment, the number of PCR tubes 126 is set to 4. Of course, in some other embodiments, the number of PCR tubes 126 can be set to at least 1 according to actual needs, which will not be repeated. The 4 PCR tubes 126 in the present embodiment are arranged in a row. The PCR amplification area 112 in the present embodiment is also provided with a tube cap loading slot 128 for placing PCR tube caps 127, and the tube cap loading slots 128 are arranged in a one-to-one correspondence with the PCR tubes 126. During the PCR amplification process, the PCR tube caps 127 are covered on the corresponding PCR tubes 126 to keep the temperature in the PCR tubes 126 stable. In the present embodiment, the 4 tube cap loading slots 128 are arranged in a row.

[0060] The reagent tube for loading reagent is installed in the reagent loading area 113. The reagent tube of the present embodiment is set to two rows, and each row of reagent tubes is arranged as 4, wherein the 4 reagent tubes in one row are for loading the PCR reagent tube 129 of PCR reagent, and in the 4 reagent tubes in another row, one of the reagent tubes is for loading the internal standard reagent tube 130 of internal standard liquid, and the remaining reagent tubes are standby. That is, the reagent tube of the present embodiment comprises the internal standard reagent tube 130 for loading the internal standard liquid and the PCR reagent tube 129 for loading the PCR reagent, and the PCR reagent tube 129 is arranged one by one with the PCR tube 126.

[0061] Specifically, the number of PCR tubes 126 in this embodiment is set to 4, and correspondingly, the number of second tip storage tubes 125 is set to 4. In this embodiment, the sample storage tube 114, the waste liquid storage tube 115 and the proteinase K storage tube 116 are arranged in the first row along the X direction, and the sample storage tube 114 is located between the waste liquid storage tube 115 and the proteinase K storage tube 116. The number of cleaning liquid storage tubes 120 is set to 3, and the three cleaning liquid storage tubes 120 and the one lysis liquid storage tube 117 are arranged in the second row along the X direction, and the three cleaning liquid storage tubes 120 are arranged adjacent to each other. The paraffin oil storage tube 118, the diluent storage tube 119, the eluent storage tube 121 and the first tip storage tube 124 are arranged in the third row along the X direction. The four second tip storage tubes 125 are arranged in the fourth row along the X direction. The two rows of reagent tubes are arranged in the 5th row and the 6th row respectively along the X direction, wherein the row of reagent tubes where the internal standard reagent tubes 130 are located is arranged in the 5th row, and the row of reagent tubes used as PCR reagent tubes 129 is arranged in the 6th row. Of course, in some other embodiments, the row of reagent tubes where the internal standard reagent tubes 130 are located can also be arranged in the 6th row, and the row of reagent tubes used as PCR reagent tubes 129 can be arranged in the 5th row, which will not be repeated. In this embodiment, the four PCR tubes 126 are arranged in the 7th row along the X direction, and the four tube cap loading slots 128 are arranged in the 8th row along the X direction. Specifically, in some other embodiments, the four PCR tubes 126 can also be arranged in the 8th row, and the four tube cap loading slots 128 can be arranged in the 7th row. In this embodiment, the second tip head storage tube 125, the PCR reagent tube 129 and the tube cap loading slot 128 are all arranged in a one-to-one correspondence with the PCR tube 126, and the correspondingly arranged second tip head storage tube 125, the PCR reagent tube 129, the tube cap loading slot 128 and the PCR tube 126 are located on the same straight line parallel to the Y direction, which is convenient for pipetting operations using the tip head during nucleic acid extraction and PCR amplification.

[0062] like Figure 2-3As shown, the cartridge 100 of this embodiment includes a cartridge body 110 and a reagent plate 150. A nucleic acid extraction area 111, a PCR amplification area 112 and a reagent loading area 113 are provided in the cartridge body 110. The reagent loading area 113 is located between the nucleic acid extraction area 111 and the PCR amplification area 112. Specifically, a reagent plate mounting structure for detachably mounting the reagent plate 150 is provided in the reagent loading area 113. The reagent plate 150 is detachably mounted in the reagent loading area 113 through the reagent plate mounting structure. Specifically, the reagent plate mounting structure can be implemented in a variety of existing ways, such as fixing the reagent plate 150 in the reagent loading area 113 with screws. In this embodiment, the reagent plate installation structure is a first installation groove 131 arranged in the reagent loading area 113, and a card slot (not shown in the figure) for engaging with the reagent plate 150 is arranged in the first installation groove 131, and a clip 141 engaging with the card slot is arranged on the reagent plate 150, so that the reagent plate 150 can be detachably installed in the first installation groove 131 through the engaging relationship between the card slot and the clip 141. Specifically, in this embodiment, the reagent tube is arranged on the reagent plate 150, and after the reagent plate 150 is installed in the first installation groove 131, the reagent tube can be fixed in the reagent loading area 113. The bottom of the first installation groove 131 is provided with a first installation hole 132 corresponding to the reagent tube. In this embodiment, the first installation hole 132 is arranged one-to-one with the reagent tube, and the first installation hole 132 is used to accommodate the corresponding reagent tube. Of course, in some other embodiments, the first mounting hole 132 can be set to be large enough and set to one, so that the first mounting hole 132 can accommodate all the reagent tubes, and can also meet the requirements of installing the reagent plate 150 and the reagent tubes. By separating the reagent plate 150 from the cartridge body 110 and arranging the reagent tubes on the reagent plate 150, the reagent plate 150 and the reagents loaded in the reagent tubes can be replaced according to the actual detection requirements, which can effectively improve the versatility of the cartridge.

[0063] In this embodiment, a second installation groove 133 is provided in the nucleic acid extraction area 111, and the sample storage tube 114 and the waste liquid storage tube 115 are both arranged in the second installation groove 133, and an identification plate 144 for identifying the position of the sample storage tube 114 is installed on the second installation groove 133, and the identification plate 144 is provided with through holes 134 and through holes 135 corresponding to the sample storage tube 114 and the waste liquid storage tube 115, respectively.

[0064] In this embodiment, a recessed recess 136 is provided in the PCR amplification area 112, and the PCR tube 126 is disposed at the bottom of the recess 136. In this embodiment, a second mounting hole 137 is provided at the bottom of the recess 136, and the PCR tube 126 is mounted in the second mounting hole 137. Of course, in some other embodiments, the PCR tube 126 and the cartridge body 110 may be integrated, that is, the PCR tube 126 may be integrally formed at the bottom of the recess 136. In this embodiment, the tube cap loading slot 128 is disposed at the bottom of the recess 136.

[0065] In this embodiment, the storage tubes, reagent tubes, PCR tubes 126 and tube cap loading slots 128 are arranged at equal intervals in the Y direction, that is, in this embodiment, among 4 rows of storage tubes, 2 rows of reagent tubes, 1 row of PCR tubes 126 and one row of tube cap loading slots 128, the row spacing between any two adjacent rows is equal, the tip storage tube is located between the sample storage tube 114 and the reagent tube, and the minimum spacing between the tip storage tube and the sample storage tube 114 in the Y direction satisfies:

[0066] D=nd

[0067] Wherein, D is the minimum spacing between the tip storage tube and the sample storage tube 114 in the Y direction; d is the spacing between two adjacent rows of storage tubes arranged along the Y direction; n is a positive integer greater than or equal to 1, and: when the tube cap loading slot 128 is set on the side of the PCR tube 126 facing away from the nucleic acid extraction area 111, n≥2, that is, at this time, the minimum spacing between the tip storage tube and the sample storage tube 114 in the Y direction is required to be greater than or equal to 2 times the row spacing; when the tube cap loading slot 128 is set on the side of the PCR tube 126 facing the nucleic acid extraction area 11, n≥1, that is, at this time, the minimum spacing between the tip storage tube and the sample storage tube 114 in the Y direction is required to be greater than or equal to 1 times the row spacing. Such a setting can avoid interference between the cover plate and the cartridge during nucleic acid detection. In a preferred implementation of this embodiment, the PCR tube 126 is symmetrically arranged relative to a straight line passing through the center of the sample storage tube 114 and parallel to the Y direction, or the center of the PCR tube 126 falls on a straight line passing through the sample storage tube 114 and parallel to the Y direction. In this embodiment, the number of PCR tubes 126 is set to 4 and arranged in a row along the X direction. The PCR tubes 126 are symmetrically arranged with respect to the straight line passing through the center of the sample storage tube 114 and parallel to the Y direction. In other embodiments, if there is only one PCR tube 126, the center of the PCR tube 126 is set to fall on the straight line passing through the sample storage tube 114 and parallel to the Y direction; if there are three PCR tubes 126, the center of the middle PCR tube 126 falls on the straight line passing through the sample storage tube 114 and parallel to the Y direction, and the two PCR tubes 126 on both sides are symmetrically arranged with respect to the straight line passing through the center of the sample storage tube 114 and parallel to the Y direction. In this way, during the PCR amplification process, the PCR tube caps 127 loaded on all PCR tubes 126 can be pressed simultaneously by the cover plate.

[0068] like Figure 3-4As shown, the cartridge 100 of this embodiment includes a cartridge body 110 and a reagent plate 150. The cartridge body 110 is provided with a nucleic acid extraction area 111, a PCR amplification area 112 and a reagent loading area 113. The reagent loading area 113 is located between the nucleic acid extraction area 111 and the PCR amplification area 112. The cartridge body 110 is provided with cartridge side panels 138 on both sides, and the two cartridge side panels 138 are provided with clearance holes 139, respectively. The clearance holes 139 are provided with a clamping plate 140. The bottom of the clamping plate 140 is fixedly connected or integrally arranged with the corresponding cartridge side panel 138, and the clamping plate 140 is provided with a clamping clip 141 protruding outward. The bottom of the clamping plate 140 of this embodiment is integrally arranged with the corresponding cartridge side panel 138. In the preferred implementation of this embodiment, the bottom surface of the card clip 141 is provided with a guide slope 142. When the card box body 110 is installed in the card box mounting seat, the matching relationship between the guide slope 142 and the card box mounting seat can be used to drive the card plate 140 to gradually bend and deform inward to achieve the card box body 110 being installed in the card box mounting seat to prevent jamming. In the preferred implementation of this embodiment, the card clip 141 is arranged in the middle of the card plate 140, and the upper outer surface of the card plate 140 is provided with an anti-skid structure 143. The anti-skid structure 143 can be implemented in various existing ways. In this embodiment, the anti-skid structure 143 is a plurality of anti-skid grooves or anti-skid strips arranged on the card plate 140. The anti-skid grooves or anti-skid strips of this embodiment are parallel to the top surface of the card box body. In some other embodiments, the anti-skid grooves or anti-skid strips can also be staggered to form anti-skid lines. By providing the anti-skid structure 143, when the card box body 110 is taken out from the card box mounting seat, the operator's hands respectively apply an inward force to the anti-skid structure 143, driving the clamping plate 140 to bend and deform inward, thereby contacting the clamping fitting relationship between the card box body 110 and the card box mounting seat, and the card box body 110 can be easily taken out from the card box mounting seat. The clamping clip 141 can be implemented in a variety of ways. The clamping clip 141 of this embodiment is set as one and is formed into a long strip, and the length direction of the clamping clip 141 is parallel to the top surface of the card box body 110. Of course, in some other embodiments, the clamping clip 141 can be set as a plurality of intervals, and all the clamping clips 141 are located on a straight line parallel to the top surface of the card box body 110.

[0069] like Figure 5-6As shown, the nucleic acid detector of this embodiment includes a base, on which a base module 300 and a pipetting module 400 are installed. Specifically, the base of this embodiment includes an upper base plate 201 and a lower base plate 202, the lower base plate 202 is located below the upper base plate 201, and a support column 203 is provided between the upper base plate 201 and the lower base plate 202, so that a base space 204 is formed between the base 201 and the lower base plate 202, and a power supply component 205 is installed in the base space 201 of this embodiment. A support frame 206 is installed on the base, and a display screen component 207 is installed on the support frame 206. Specifically, in this embodiment, the base module 300 and the pipetting module 400 are both installed on the base. In this embodiment, the base module 300 is set to two, and the two base modules 300 can install two cartridges 100 and perform nucleic acid detection.

[0070] The base module 300 of this embodiment includes a base assembly 310, an in-and-out cabin assembly 350, a PCR temperature control assembly 370, and a photoelectric module 390. The base assembly 310 is used to load the cartridge 100 and perform temperature control during the nucleic acid extraction process. The in-and-out cabin assembly is used to drive the base assembly to move horizontally between the out-and-in cabin position and the in-and-in cabin position to replace the cartridge 100. The PCR temperature control assembly is used to perform temperature control during the PCR process.

[0071] like Figure 7-9As shown, the base assembly 310 of this embodiment includes a mounting base 311, on which a temperature control assembly for temperature control during the nucleic acid extraction process is provided, and above the temperature control assembly is provided a cartridge mounting seat 312 for mounting the cartridge 100. Specifically, the temperature control assembly of this embodiment includes a temperature control element 313 for adjusting the temperature and a heat dissipation assembly, on which a variable temperature metal 314 is provided, and on which a hot well 315 is provided. The heat dissipation assembly of this embodiment is arranged below the temperature control element 313. In this embodiment, a temperature control chamber 317 is formed between the temperature control assembly and the bottom surface of the cartridge mounting seat 312, and the hot well 315 is located in the temperature control chamber 317. An upper opening 318 is provided on the top surface of the temperature control chamber 317, and the upper opening 318 is used for the sample storage tube 114 of the cartridge 100 to pass through and fall into the hot well 315, so as to use the hot well 315 to control the temperature of the sample storage tube 114, so as to meet the temperature control requirements of nucleic acid extraction. In this embodiment, in order to reduce the space of the temperature control chamber 317 and improve the temperature control accuracy and efficiency, a temperature control groove 319 located above the heat well 315 is provided on the bottom surface of the cartridge mounting seat 312. The heat well 315 extends upward into the temperature control groove 319, and the upper opening 318 is provided on the top surface of the temperature control groove 319. In this embodiment, the temperature control groove 319 is formed by the bottom surface of the cartridge mounting seat 312 being recessed upward. In this way, the gap between the bottom surface of the cartridge mounting seat 312 and the temperature control component can be set smaller, thereby reducing the space size of the temperature control chamber 317 and improving the accuracy and efficiency of temperature control during nucleic acid extraction. A radio frequency card reader 323 is installed on the cartridge mounting seat 312 of this embodiment.

[0072] Specifically, the heat dissipation assembly 316 includes a heat sink 320, the temperature control element 313 is mounted on the top surface of the heat sink 320, and a heat sink 321 is provided on the bottom surface of the heat sink 320. Specifically, a temperature control mounting seat 322 is mounted on the mounting base 311, and a heat dissipation channel 324 is formed in the gap between the heat sink 320 and the temperature control mounting seat 322. In the preferred implementation of this embodiment, the heat sink 3 is parallel to the heat dissipation channel 324 to improve the heat dissipation efficiency. In the preferred implementation of this embodiment, a mounting through hole 325 is correspondingly provided between the bottom surface of the temperature control mounting groove and the mounting base 311, and a heat dissipation fan 326 is installed in the mounting through hole 325 to increase the airflow velocity in the heat dissipation channel 324, thereby improving the heat dissipation efficiency. In this embodiment, the card box mounting seat 312 is fixedly mounted on the heat sink 320, and a pressing block 327 for pressing and fixing the temperature-changing metal 314 on the temperature control element 313 is installed on the heat sink 320.

[0073] The base assembly of the present embodiment also includes a magnetic attraction assembly, which includes two mounting arms 328 respectively located on both sides of the hot well 315 and a magnetic attraction guide assembly for driving the two mounting arms 328 to move in the vertical direction, and magnets 329 are respectively installed on the two mounting arms 328. The magnetic attraction guide assembly of the present embodiment includes a magnetic attraction guide rod 330 located in the vertical direction, a magnetic attraction slider 331 slidably mounted on the magnetic attraction guide rod 330, and a magnetic attraction control mechanism for controlling the magnetic attraction slider 331 to move along the magnetic attraction guide rod 330. Specifically, the magnetic attraction control mechanism controls the magnetic attraction slider 331 to move along the magnetic attraction guide rod 330 and has two positioning points, a high position and a low position, and the high position is located above the low position. Both mounting arms 328 are fixedly mounted on the magnetic attraction slider 331 and move synchronously with the magnetic attraction slider 331 in the vertical direction. When the magnetic slider 331 is in a low position, the magnet 329 does not generate magnetic force or generates a small magnetic force on the magnetic beads in the sample storage tube 114; when the magnetic slider 331 is in a high position, the magnet 329 generates a magnetic attraction effect on the magnetic beads in the sample storage tube 114. Further, the magnetic control mechanism for controlling the magnetic slider 331 to move along the magnetic guide rod 330 to control the position of the magnetic slider 331 can be implemented in a variety of existing ways, such as a screw mechanism, a gear rack mechanism, and a synchronous belt mechanism. The magnetic control mechanism of this embodiment includes a magnetic reset spring 332 sleeved on the magnetic guide rod 330 and a magnetic pull rod 333 for driving the magnetic slider 331 to move upward along the magnetic guide rod 330. The magnetic reset spring 332 of this embodiment is located between the magnetic slider 331 and the top of the magnetic guide rod 330 and applies a downward elastic force to the magnetic slider 331. The middle part of the magnetic attraction lever 333 of this embodiment is rotatably matched with a fixed shaft 334, a horizontal slide groove 335 is provided on the magnetic attraction slider 331, and a matching shaft 336 is provided at the first end of the magnetic attraction lever 333 corresponding to the horizontal slide groove 335, and the matching shaft 336 can rotate relative to the magnetic attraction slider 331 and can move along the horizontal slide groove 335. In this way, the magnetic attraction lever 333 is driven to rotate, and the matching relationship between the matching shaft 336 and the horizontal slide groove 335 can be used to drive the magnetic attraction slider 331 to move along the magnetic attraction guide rod 330, so that the position of the magnetic attraction slider 331 on the magnetic attraction guide rod 330 can be adjusted, so that the magnetic attraction slider 331 moves between a high position and a low position along the magnetic attraction guide rod 330.

[0074] The card box mounting seat 312 of the present embodiment includes a bottom plate 337, and the temperature control groove 319 is arranged on the bottom plate 337. Two base side plates 338 parallel to each other are arranged on the bottom plate 337, and the distance between the inner sides of the two base side plates 338 is equal to or slightly larger than the distance between the outer sides of the two card box side plates 138, and a snap-in groove or a snap-in hole 339 cooperating with the snap-in clip 141 is arranged on the base side plate 338. In the present embodiment, the distance between the top surface of the snap-in groove or the snap-in hole 339 and the bottom plate 337 is equal to or slightly larger than the distance between the top surface of the snap-in clip and the bottom surface of the card box side plate, which can prevent the card box 100 from loosening in the vertical direction after being installed in the card box mounting seat 312. In the present embodiment, a snap-in hole 339 cooperating with the snap-in clip 141 is arranged on the base side plate 338. As Figure 10-12 The figure shows the process of taking the card box 100 out of the card box mounting seat 312.

[0075] like Fig.13 As shown, the cabin entry and exit assembly 350 includes a cabin entry and exit track 351 installed on the base, a cabin entry and exit slider 352 slidably matched with the cabin entry and exit track 351 is installed on the cabin entry and exit slider 352, a base mounting plate 353 is installed on the cabin entry and exit slider 352, and the base assembly 310 is installed on the base mounting plate 353. The base is provided with a cabin entry and exit driving mechanism for driving the cabin entry and exit slider 352 to move between the cabin exit position and the cabin entry position along the cabin entry and exit track 351. The cabin entry and exit driving mechanism of this embodiment includes a cabin entry and exit driving motor 354 and two synchronous pulleys 355 respectively located at both ends of the cabin entry and exit track 351, a synchronous belt 356 is provided between the two synchronous pulleys 355, the synchronous belt 356 is fixedly connected to the cabin entry and exit slider 352, and the cabin entry and exit driving motor 354 is in transmission connection with one of the synchronous pulleys 355. In this embodiment, the cabin entry and exit driving motor 354 is in transmission connection with a synchronous pulley 355 located at the rear end of the cabin entry and exit track 351. In this embodiment, the cabin entry and exit rails 351 are configured as two parallel to each other, and the cabin entry and exit slider 352 is slidably matched with the two cabin entry and exit rails 351 .

[0076] like Figure 14-15As shown, the cabin entry and exit assembly of this embodiment also includes a cabin door assembly. Specifically, the cabin door assembly includes a cabin door 357 located at the front end of the cabin entry and exit track 351 and a cabin door reset mechanism for resetting the cabin door 357 after opening and keeping the cabin door in a normally closed state, and the cabin door 357 is hingedly connected to the base. When the cabin entry and exit slider 352 is located at the cabin exit position, the cabin door 357 rotates and opens under the action of the base mounting plate 353; when the cabin entry and exit slider 352 is located at the cabin entry position, the cabin door 357 is closed under the action of the reset mechanism. Specifically, a double-hinged connecting rod 358 is provided between the cabin door 357 and the base, and the first end of the double-hinged connecting rod 358 is hingedly connected to the cabin door 357 through a first hinge shaft, and the second end is hingedly connected to the base through a second hinge shaft. In the preferred implementation of this embodiment, a reinforcing plate 359 is provided below the base mounting plate 353, and a bending section 360 is provided at the front end of the reinforcing plate 359, which is bent upward and located in front of the base mounting plate 353. Specifically, the front side of the bending section 360 is set as a transition curved surface that smoothly transitions with the bottom surface of the reinforcing plate 359, or a transition slope is provided on the front side of the bending section 360. Comparing the acute angle between the transition slope and the horizontal plane, when the transition slope is set to at least two sections, the angle between the upper transition slope and the horizontal plane of any two adjacent transition slopes is greater than the angle between the lower transition slope and the horizontal plane. In this embodiment, a transition slope is provided on the front side of the bending section 360, and the transition slope is set to two sections. In the preferred implementation of this embodiment, the first hinge shaft and the second hinge shaft are respectively provided with a first roller 361 and a second roller 362 that roll with the front side of the bending section 360 and the bottom surface of the reinforcing plate 359 to reduce resistance. The door reset mechanism of this embodiment includes a door reset spring 363, which is connected to the lower end of the door 357 and applies an elastic force to close the door 357. Specifically, the upper end of the hatch door return spring 363 of the embodiment is connected to the lower end of the hatch door 357, and the lower end of the hatch door return spring 363 is fixedly connected to the lower bottom plate 202. In order to ensure that the hatch door return spring 363 applies the elastic force required to close the hatch door 357 to the hatch door 357, it is at least necessary to ensure that the axis of the hatch door return spring 363 is always located at the rear side of the first hinge shaft and the second hinge shaft during the entire process of opening and closing the hatch door 357. In order to meet this condition, the embodiment is provided with a bent connecting section 364 at the lower end of the hatch door 357, and the upper end of the hatch door return spring 363 is connected to the bent connecting section 364. Specifically, the hatch door 357 cooperates with the support frame 206 when it is closed.

[0077] like Figure 16-18As shown, the PCR temperature control assembly 370 includes a PCR temperature control unit 371 and a temperature control drive assembly for driving the PCR temperature control unit 371 to move between the avoidance position and the temperature control position along the vertical direction. The temperature control drive assembly is used to drive the PCR temperature control unit 317 to move along the vertical direction and has two positioning points, the avoidance position and the temperature control position, and the avoidance position is located below the temperature control position; when the PCR temperature control unit 371 is located at the avoidance position, the PCR temperature control unit 371 is misaligned with the card box 100 installed on the base assembly 310, and will not interfere with the card box 100, that is, it will not affect the movement of the base assembly 310 in and out of the cabin, as shown in FIG. Fig.19 When the PCR temperature control unit 371 is in the temperature control position, the PCR temperature control unit 371 performs temperature control on the PCR tube 126 in the cartridge 100, as shown in FIG. Fig. 20 In this embodiment, the PCR temperature control unit 371 includes a PCR temperature control element 372 and a PCR heat dissipation component. A PCR temperature-changing metal 373 is provided above the PCR temperature control element 372. A PCR hot well 374 is provided on the PCR temperature-changing metal 373 in one-to-one correspondence with the PCR tubes 126 of the cartridge 100. The PCR heat dissipation component is provided below the PCR temperature control element 372. Specifically, the PCR heat dissipation component includes a heat dissipation seat 375 and a heat dissipation channel 376 located below the heat dissipation seat 375. A heat sink 377 is provided at the bottom of the heat dissipation seat 375. A heat dissipation fan 377a for accelerating the airflow velocity is also provided in the heat dissipation channel 376.

[0078] The temperature control drive assembly of this embodiment includes a fixed plate 378, on which a lifting track 379 is provided in the vertical direction, on which a lifting slider 380 is installed, and the lifting slider 380 is fixedly connected to the temperature control unit 371. A lifting motor 381 is also installed on the fixed plate 378, and a screw rod 382 is transmission-connected to the output shaft of the lifting motor 381, and the screw rod 382 is parallel to the lifting track 379. A lifting seat 383 is fixedly connected to the temperature control unit 371, and the lifting seat 383 is threadedly matched with the screw rod 382.

[0079] The PCR temperature control unit 371 of this embodiment is provided with a driving arm 384, and the driving arm 384 is used to contact and cooperate with the second end of the magnetic suction lever 333 to drive the magnetic suction lever 333 to rotate around the fixed axis 334. In the preferred implementation of this embodiment, a driving wheel 385 for contacting and cooperating with the magnetic suction lever 333 is installed on the driving arm 384. In order to use the driving arm 384 to drive the magnetic suction slider to move between the high position and the low position, this embodiment sets a positioning point below the avoidance position on the path where the temperature control drive component drives the PCR temperature control unit 371 to move in the vertical direction, and the positioning point is named the magnetic suction position. Specifically, when the PCR temperature control unit 371 moves between the magnetic position and the avoidance position, the driving arm 384 contacts and cooperates with the magnetic pull rod 333, and the lifting and lowering movement of the PCR temperature control unit 371 between the magnetic position and the avoidance position can drive the magnetic slider 331 to move between the high position and the low position; when the PCR temperature control unit 371 moves between the avoidance position and the temperature control position, the driving arm 384 disengages from the magnetic pull rod 333, that is, the magnetic slider 331 remains stationary during the movement of the PCR temperature control unit 371 between the avoidance position and the temperature control position; when the PCR temperature control unit 371 is in the magnetic position, the magnet 329 is in the high position, such as Fig.21 When the PCR temperature control unit 371 is in the avoidance position, the magnet 329 is in the low position, as shown in FIG. Fig. 22 shown.

[0080] A driving arm 384 is provided on the PCR temperature control unit 371, and the driving arm 384 cooperates with the second end of the magnetic attraction lever 333, so as to drive the magnetic attraction component to move while driving the PCR temperature control unit 371 to move up and down. Specifically, when the PCR temperature control unit 371 moves between the magnetic position and the avoidance position, the driving arm 384 contacts and cooperates with the magnetic suction rod 333, and the magnetic suction rod 333 can be used to drive the magnet 329 to move along the magnetic guide rod 330; when the PCR temperature control unit 371 moves between the avoidance position and the temperature control position, the driving arm 384 disengages from the magnetic suction rod 333, that is, the avoidance position of the PCR temperature control unit 371 is the critical position of the contact and cooperation between the driving arm 384 and the magnetic suction rod 333, and when the PCR temperature control unit 384 is in the avoidance position, the disengagement and cooperation between the driving arm 384 and the magnetic suction rod 333 can be achieved, and the PCR temperature control unit 371 and the card box 100 loaded on the base assembly 310 can be displaced in the vertical direction, which will not affect the entry and exit movement of the base assembly 310 The movement causes interference. At this time, the driving arm 384 will not apply force to the magnetic attraction lever 333, and the magnet 329 is in a low position. The magnet 329 will not generate a magnetic force on the magnetic beads in the sample or the generated magnetic force is relatively small; when nucleic acid is extracted from the sample, the PCR temperature control unit 371 moves to the magnetic attraction position, and the magnetic attraction lever 333 is used to drive the magnet 329 to move to a high position, thereby generating a magnetic force on the magnetic beads in the sample; when PCR amplification is performed, the PCR temperature control unit 371 moves to the temperature control position, thereby achieving the technical purpose of temperature control of the PCR tube 126 arranged in the cartridge 100, so as to meet the requirements of PCR amplification; that is, this embodiment realizes the linkage control between the PCR temperature control component 370 and the base component 310, which can not only meet the requirements of nucleic acid extraction and PCR amplification, but also simplify the structure.

[0081] like Fig.23As shown, an excitation light fiber 391 and a receiving light fiber 392 are respectively provided between the photoelectric module 390 and each PCR hot well 374, and a fiber positioning assembly 386 for positioning the excitation light fiber 391 and the receiving light fiber 392 is provided on the PCR temperature control unit 371. Specifically, the photoelectric module 390 of this embodiment includes a photoelectric mounting seat 393 fixedly mounted on the base, and a fiber connecting plate 394 and a photoelectric detector 395 are provided on the photoelectric mounting seat 393. A group of fiber connecting ports are provided on the fiber connecting plate 394 corresponding to each PCR hot well 374. The photoelectric detector moves along the fiber connecting plate 394, and excites and receives light signals for the excitation light fiber 391 and the receiving light fiber 392 corresponding to the same PCR hot well 374. Specifically, a photoelectric detection driving assembly 396 for driving the photoelectric detector 395 to move along the fiber connecting plate 394 is provided on the photoelectric mounting seat 393. The photoelectric detection drive component 396 is a linear drive mechanism, which can be implemented in a variety of existing ways. The photoelectric detection drive component 396 of this embodiment adopts a threaded screw mechanism.

[0082] like Figure 24-27The pipetting module 400 of the present embodiment includes a pipetting support frame 401, on which a pipetting unit is installed. The pipetting unit is arranged in a one-to-one correspondence with the base module 300. The base module 300 of the present embodiment is set to two, that is, the pipetting unit is also correspondingly provided with two. The pipetting unit of the present embodiment includes a horizontal track seat 402 fixedly mounted on the pipetting support 401, a horizontal track 403 is provided on the horizontal track seat 402, a horizontal slider 404 is installed on the horizontal track 403 to slide with it, and a tip head replacement assembly is installed on the horizontal slider 404. The tip head replacement assembly of the present embodiment includes a track seat 405 fixedly mounted on the horizontal slider 404, a vertical track 406 is provided on the track seat 405, and a moving seat 407 that slides with it is installed on the vertical track 406, that is, the moving seat 407 of the present embodiment can move in the vertical direction relative to the track seat 405. A connecting gun 408 is installed on the moving seat 407, and the connecting gun 408 of the present embodiment is located in the vertical direction. The lower end of the connecting gun 408 is provided with a connecting gun head for cooperating with the tip head, and the connecting gun head is provided with at least one convex ring 409 for interference fit with the tip head. In this embodiment, two convex rings 409 are provided on the connecting gun head. A mounting through hole 410 is provided in the movable seat 407 of this embodiment, and the connecting gun 408 is fixedly installed in the mounting through hole 410. Specifically, a compression connector 411 is provided at the top of the mounting through hole 410 to be threadedly matched therewith, and the connecting gun 408 is compressed and fixed in the mounting through hole 410 by the compression connector 411. Specifically, in this embodiment, the horizontal track 403 is located in the X direction and is perpendicular to the in-and-out cabin track 351, that is, the in-and-out cabin track 351 of this embodiment is located in the Y direction, and the vertical track 406 is located in the Z direction. In this way, under the joint action of the liquid transfer unit and the in-and-out cabin assembly, the connecting gun 408 can be driven to move relative to the base assembly in the X direction, the Y direction and the Z direction to meet the transfer requirements of liquids such as samples and reagents during nucleic acid extraction and PCR amplification.

[0083] A tip head pick-and-place control assembly is provided between the connecting gun 408 and the movable seat 407 of the present embodiment. Specifically, the tip head pick-and-place control assembly of the present embodiment includes a gun head cover 412, a pick-and-place control mechanism and a telescopic rod 433. Specifically, the gun head cover 412 of the present embodiment is sleeved outside the connecting gun 408 and is used to cooperate with the tip head limit. The pick-and-place control mechanism is used to guide the gun head cover 412 to move in the vertical direction and make the gun head cover 401 move downward relative to the lowest position of the connecting gun 408 to reach the connecting gun head or reach below the connecting gun head, so that the tip head loaded on the connecting gun head can be unloaded. Specifically, the pick-and-place control mechanism of the present embodiment includes a guide rod 413 parallel to the connecting gun 408, and the gun head cover 412 moves synchronously with the guide rod 413. Specifically, a connecting seat 414 is provided on the gun head cover 412 of the present embodiment, and the guide rod 413 is fixedly connected to the connecting seat 414. The movable seat 407 of this embodiment is provided with a guide hole 415 that is slidably matched with the guide rod 413, the upper end of the guide rod 413 extends out of the guide hole 415, and the upper end of the guide rod 413 is provided with a limit structure 416 that is limitedly matched with the movable seat 407. In this way, when the connecting gun 408 is loaded with a tip head, the assembly force of the interference fit between the tip head and the connecting gun 408 is used to support the gun head cover 412, and the lower end of the gun head cover 412 is limitedly matched with the tip head, and the gun head cover 412 at this time also has the ability to continue to move downward relative to the connecting gun 408. The gun head cover 412 is provided with a limit groove 417, and the limit groove 417 of this embodiment is located in the vertical direction. The telescopic rod 433 is installed on the track seat 405, and the telescopic rod 433 can extend into the limit groove 417 when extended, and is located outside the limit groove 417 when retracted. The telescopic rod 433 of this embodiment is an electromagnetic telescopic rod, and the limiting groove 417 of this embodiment is arranged in the connecting seat 414.

[0084] When the tip head needs to be unloaded, the telescopic rod 433 is controlled to extend and extend into the limiting groove 417, driving the movable seat 407 to move upward along the vertical track 406. Under the limiting matching relationship between the gun head cover 412 and the tip head, the gun head cover 412 first moves upward together with the connecting gun 408; when the telescopic rod 433 is limitedly matched with the lower end of the limiting groove 417, the gun head cover 412 can no longer move upward. At this time, the connecting gun 408 continues to be driven to move upward, and the gun head cover 412 moves downward relative to the connecting gun 408, while overcoming the assembly force between the tip head and the connecting gun 408, so that the tip head is separated from the connecting gun head to achieve the purpose of unloading the tip; after the tip head is unloaded, the telescopic rod 433 is controlled to retract outside the limiting groove 417. At this time, the gun head cover 412 continues to move downward relative to the connecting gun 408 under the action of gravity until the guide rod 413 is limitedly matched with the movable seat 407.

[0085] In the preferred implementation of this embodiment, a compression spring 418 is provided between the guide rod 413 and the movable seat 407 to apply a downward elastic force to the guide rod 413. In this way, when the connecting gun 408 is loaded with a tip head, the assembly force of the interference fit between the tip head and the connecting gun 408 not only plays a role in supporting the gun head cover 412, but also plays a compressive role on the compression spring 418; when the tip head is unloaded from the connecting gun 408, the gun head cover 412 continues to move downward relative to the connecting gun 408 under the action of gravity and the elastic force of the compression spring 418 until the guide rod 413 and the movable seat 407 are limitedly matched. Specifically, in this embodiment, the upper end of the guide rod 413 is provided with a small-diameter guide rod section 413a, and the lower end is provided with a large-diameter guide rod section 413b. The outer diameter of the large-diameter guide rod section 413b is greater than the outer diameter of the small-diameter guide rod section 413a. The compression spring 418 is installed between the movable seat 407 and the large-diameter guide rod section 413b. The guide through hole 415 of this embodiment is provided with a large diameter guide hole section 415a at the lower end and a small diameter guide hole section 415b at the upper end. The inner diameter of the large diameter guide hole section 415a is larger than the inner diameter of the small diameter guide hole section 415b. The large diameter guide hole section 415a cooperates with the large diameter guide rod section 413b, and the small diameter guide rod section 413a cooperates with the small diameter guide hole section 415b and extends upward above the small diameter guide hole section 415b. The limiting structure 416 is provided on the small diameter guide rod section 413a, and the compression spring 418 is installed in the large diameter guide hole section 415a.

[0086] Specifically, the liquid transfer unit also includes a liquid transfer drive assembly for driving the mobile seat 407 to move, and the liquid transfer drive assembly includes a horizontal drive mechanism for driving the horizontal slider 404 to move along the horizontal track 403 and a vertical drive mechanism for driving the mobile seat 407 to move along the vertical track 406. Specifically, the horizontal drive mechanism and the vertical drive mechanism of this embodiment both adopt a threaded screw mechanism. Specifically, the horizontal drive mechanism includes a first screw rod 419 parallel to the horizontal track 403 and a first motor 420 connected to the first screw rod 419 in a transmission manner, and the horizontal slider 404 is threadedly matched with the first screw rod 419. The vertical drive mechanism includes a second screw rod 421 parallel to the vertical track 406 and a second motor 422 connected to the second screw rod 421 in a transmission manner, and the mobile seat 407 is threadedly matched with the second screw rod 421. Specifically, the track seat 402 of this embodiment is equipped with an optical coupling sensor 423 for detecting the position of the mobile seat 407 in the vertical direction, and the mobile seat 407 is equipped with a light shielding plate 424 matched with the optical coupling sensor 423.

[0087] The principle of the tip head pick-and-place control component of this embodiment is as follows: by setting up the tip head pick-and-place control component, by setting a gun head cover 412 on the connecting gun 408 to cooperate with the tip head limit, the pick-and-place control mechanism is used to guide the gun head cover 412 to move in the vertical direction, and the gun head cover 412 moves downward relative to the connecting gun 408 to the lowest position to reach the connecting gun head or reach below the connecting gun head, so that the tip head loaded on the connecting gun head can be unloaded; at the same time, the limiting matching relationship between the telescopic rod 433 and the limiting groove 417 of the gun head cover 412 is used to drive the gun head cover 412 to move relative to the connecting gun 408; in this way, when the tip head needs to be unloaded, the telescopic rod 433 is controlled to extend and extend into the limiting groove 417, driving the moving seat 407 to move upward along the vertical track, and under the limiting matching relationship between the gun head cover 412 and the tip head, the gun head The sleeve 412 first moves upward with the connecting gun 408; when the telescopic rod 433 is limitedly engaged with the lower end of the limiting groove 417, the gun head sleeve 412 can no longer move upward. At this time, the connecting gun 408 continues to be driven upward, and the gun head sleeve 412 moves downward relative to the connecting gun 408, overcoming the assembly force between the tip head and the connecting gun 408, so that the tip head is separated from the connecting gun head to achieve the purpose of unloading the tip; after the tip head is unloaded, the telescopic rod 433 is controlled to retract outside the limiting groove 417. At this time, the gun head sleeve 412 continues to move downward relative to the connecting gun 408 under the action of gravity until the guide rod 413 is limitedly engaged with the moving seat 407; when loading the tip, the connecting gun 408 is directly inserted into the corresponding tip head, and the tip head is loaded on the connecting gun by utilizing the interference fit relationship between the connecting gun head and the tip head.

[0088] The movable seat 407 of this embodiment is provided with a tube cap pressing plate 425, which is used to press the tube caps 127 loaded in the PCR tubes 126 during the PCR process. Specifically, the tube cap pressing plate 425 of this embodiment includes a pressing plate 425a located in the horizontal direction, and connecting plates 425b are respectively provided at both ends of the pressing plate 425a, and the connecting plates 425b are fixedly connected to the movable seat 407. In this embodiment, the area of ​​the pressing plate 425a covers the projection area of ​​the area occupied by all PCR tubes 126 on the horizontal plane, that is, the tube caps 127 loaded in all PCR tubes 126 can be pressed at one time by using the pressing plate 425a.

[0089] In this embodiment, the distance between the geometric center of the cover plate 425a and the axis of the connecting gun 408 is equal to the distance between the sample storage tube 114 and the PCR tube 126 in the cartridge 100. In combination with the above description of the cartridge 100 in this embodiment, a downwardly recessed clearance groove 136 is provided in the PCR amplification area 112 of the cartridge 100, and the PCR tube 126 and the tube cap loading groove 128 are both provided in the clearance groove 136. In the process of transferring liquid using the tip head, the height of the cover plate 425a will be lower than the top surface of the cartridge 100 only when the tip head transfers liquid at the sample storage tube 114 and when loading or unloading the tip head, as shown in FIG. Figure 28-30 As shown. That is, if no corresponding adjustment is made, when the tip head transfers liquid at the sample storage tube 114 and when loading or unloading the tip head, the pressure cover plate 425a will interfere with the card box 100. This embodiment achieves the technical purpose of avoiding the pressure cover plate 425a by providing a downwardly recessed clearance groove 136 in the PCR amplification area 112 of the card box 100. In this embodiment, among the 4 rows of storage tubes, 2 rows of reagent tubes, 1 row of PCR tubes 126 and a row of tube cap loading slots 128, the row spacing between any two adjacent rows is equal, the tip head storage tube is located between the sample storage tube 114 and the reagent tube, and the minimum spacing between the tip head storage tube and the sample storage tube 114 in the Y direction satisfies:

[0090] D = n

[0091] Wherein, D is the minimum spacing between the tip storage tube and the sample storage tube 114 in the Y direction; d is the spacing between two adjacent rows of storage tubes arranged along the Y direction; n is a positive integer greater than or equal to 1, and: when the tube cap loading slot 128 is arranged on the side of the PCR tube 126 facing away from the nucleic acid extraction area 111, n≥2, that is, at this time, the minimum spacing between the tip storage tube and the sample storage tube 114 in the Y direction is required to be greater than or equal to 2 times the row spacing; in this way, when the tip is located in the sample storage tube 114, the pressure cover plate 425a is located directly above the PCR tube 126, and under the avoidance effect of the give way slot 136, the pressure cover plate 425a will not interfere with the cartridge 100, such as Fig.29 When the connecting gun 408 is loading or unloading the tip head, since the minimum spacing between the tip head storage tube and the sample storage tube 114 in the Y direction is greater than or equal to 1 times the row spacing, the pressure cover plate 425a is located outside the rear end of the cartridge 100 and does not interfere with the cartridge 100, as shown in FIG. Fig.28Similarly, when the tube cap loading slot 128 is set on the side of the PCR tube 126 facing the nucleic acid extraction area 11, n≥1, that is, at this time, the minimum spacing between the tip storage tube and the sample storage tube 114 in the Y direction is required to be greater than or equal to 1 row spacing; in this way, when the tip is located in the sample storage tube 114, the pressure cover plate 425a is located directly above the PCR tube 126, and under the avoidance of the clearance slot 136, the pressure cover plate 425a will not interfere with the cartridge 100; when the connecting gun 408 loads or unloads the tip, because the minimum spacing between the tip storage tube and the sample storage tube 114 in the Y direction is greater than or equal to 1 row spacing, the pressure cover plate 425a is located outside the rear end of the cartridge 100 and will not interfere with the cartridge 100.

[0092] The pipetting unit of the present embodiment also includes a pipetting pipeline system. Specifically, a central through hole 426 is provided in the connecting gun 408, and the pipetting pipeline system is connected to the central through hole 426. The pipetting pipeline system of the present embodiment includes a plunger pump 427 and a three-way solenoid valve 428. Among the three connecting ports of the three-way solenoid valve 428, the first connecting port is connected to the plunger pump 427, the second connecting port is provided with an air filter 429 and communicates with the atmosphere, and a pipetting connecting tube 430 is provided between the third connecting port and the central through hole 426, the pipetting connecting tube 430 is connected to the clamping connector 411, and the pipetting connecting tube 430 is connected to an air pressure detection tube 431, and the air pressure detection tube 431 is connected to an air pressure sensor 432.

[0093] The above-described embodiments are only preferred embodiments for fully illustrating the present invention, and the protection scope of the present invention is not limited thereto. Equivalent substitutions or changes made by those skilled in the art based on the present invention are within the protection scope of the present invention. The protection scope of the present invention shall be subject to the claims.

Claims

1. A pipetting module, characterized in that: It comprises a pipetting support frame, on which at least one pipetting unit is installed, and the pipetting unit comprises a horizontal track, on which a horizontal slider slidably matched therewith is installed, on which a tip head replacement assembly is installed, and the tip head replacement assembly comprises a track seat fixedly installed on the horizontal slider, on which a movable seat is provided, and the movable seat can move along a vertical direction relative to the track seat, and on which a connecting gun is installed, the connecting gun is located in a vertical direction, and a connecting gun head for matching with a tip head is provided at the lower end of the connecting gun; A tip head pick-and-place control assembly is provided between the connecting gun and the movable seat; the tip head pick-and-place control assembly comprises a gun head cover, a telescopic rod and a pick-and-place control mechanism, the gun head cover is provided outside the connecting gun and is used to cooperate with the tip head in a limiting manner; the pick-and-place control mechanism is used to guide the gun head cover to move in a vertical direction, and the lowest position of the gun head cover moving downward relative to the connecting gun reaches the connecting gun head or reaches below the connecting gun head; a limiting groove is provided on the gun head cover, the telescopic rod is installed on the track seat, and the telescopic rod can extend into the limiting groove.

2. The liquid transfer module according to claim 1, characterized in that: The connecting gun head is provided with at least one convex ring for interference fit with the tip head.

3. The liquid transfer module according to claim 1, characterized in that: The pick-and-place control mechanism includes a guide rod parallel to the connecting gun, and the gun head cover moves synchronously with the guide rod; the movable seat is provided with a guide through hole that is slidably matched with the guide rod, and the upper end of the guide rod is provided with a limit structure that is limitedly matched with the movable seat; A compression spring for applying a downward elastic force to the guide rod is arranged between the guide rod and the moving seat.

4. The liquid transfer module according to claim 3, characterized in that: The upper end of the guide rod is provided with a small-diameter guide rod section, and the lower end is provided with a large-diameter guide rod section, the outer diameter of the large-diameter guide rod section is larger than the outer diameter of the small-diameter guide rod section; the compression spring is installed between the moving seat and the large-diameter guide rod section; the lower end of the guide through hole is provided with a large-diameter guide hole section, and the upper end is provided with a small-diameter guide hole section, the inner diameter of the large-diameter guide hole section is larger than the inner diameter of the small-diameter guide hole section; the large-diameter guide hole section cooperates with the large-diameter guide rod section, the small-diameter guide rod section cooperates with the small-diameter guide hole section and extends upward from the small-diameter guide hole section, the limiting structure is arranged on the small-diameter guide rod section, and the compression spring is installed in the large-diameter guide hole section.

5. The liquid transfer module according to claim 1, characterized in that: The connecting sleeve is provided with a connecting seat, the guiding rod is fixedly connected to the connecting seat, and the limiting groove is arranged in the connecting seat.

6. The liquid transfer module according to claim 1, characterized in that: An optical coupling sensor for detecting the position of the moving seat is installed on the track seat, and a light shielding plate cooperating with the optical coupling sensor is installed on the moving seat.

7. The liquid transfer module according to claim 1, characterized in that: A tube cap pressing plate is installed on the movable seat, and the tube cap pressing plate is used to press the tube cap loaded in the PCR tube; The tube cap pressure plate includes a pressure cover plate located in the horizontal direction, and connecting plates are respectively provided at both ends of the pressure cover plate, and the connecting plates are fixedly connected to the movable seat; the distance between the geometric center of the pressure cover plate and the axis of the connecting gun is equal to the spacing between the sample storage tube and the PCR tube in the nucleic acid detection card box.

8. The liquid transfer module according to claim 1, characterized in that: A central through hole is arranged in the connecting gun; and the pipetting unit further comprises a pipetting pipeline system connected to the central through hole.

9. The liquid transfer module according to claim 8, characterized in that: The pipetting pipeline system includes a plunger pump and a three-way solenoid valve. Among the three connecting ports of the three-way solenoid valve, the first connecting port is connected to the plunger pump, the second connecting port is provided with an air filter connected to the atmosphere, and a pipetting connecting tube is provided between the third connecting port and the central through hole, the pipetting connecting tube is connected to an air pressure detection tube, and the air pressure detection tube is connected to an air pressure sensor.

10. A nucleic acid detector, characterized in that: It includes a bottom plate, on which are mounted an in-and-out bin assembly and a pipetting module as described in any one of claims 1 to 9, the in-and-out bin assembly comprising an in-and-out bin track located in the horizontal direction and perpendicular to the horizontal track, the in-and-out bin track is mounted with an in-and-out bin slider slidably matched therewith, and the in-and-out bin slider is mounted with a base assembly; the bottom plate is provided with an in-and-out bin driving mechanism for driving the in-and-out bin slider to move along the in-and-out bin track between an exit position and an entry position.

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