Water quality purification bio-enzyme reagent and preparation method thereof

Abstract

The invention discloses a water quality purification bio-enzyme reagent and a preparation method thereof, and belongs to the technical field of environmental protection, the water quality purification bio-enzyme reagent comprises the following components by weight: 34%-42% of bio-enzyme; 5%-11% of microorganisms; 30%-40% of deionized water; 15%-20% of activated carbon; 2%-5% of tangerine powder; 5%-7% of a flocculating agent; under the synergistic effect of multiple components, the water quality purification bio-enzyme reagent can obviously improve the sewage quality, reduce the nitrogen and phosphorus content in sewage and promote pollutant degradation.

Description

Technical Field

[0001] The present invention relates to the technical field of environmental protection, and in particular to a water purification bioenzyme reagent and a preparation method thereof. Background Art

[0002] Water pollution has become one of the serious problems facing the world, posing a huge threat to the ecological environment and human health. Traditional water purification methods mainly include chemical and physical methods. However, these methods have many shortcomings in practical applications.

[0003] Chemical agents can induce chemical reactions and precipitation or decomposition of pollutants in water. However, the amount of chemical agents added is difficult to control accurately. Excessive addition will not only increase treatment costs, but also cause further damage to the environment and bring secondary pollution to water quality. Physical water purification mainly removes pollutants in water through physical processes such as filtration, precipitation, and adsorption, but its purification efficiency is relatively low, and the treatment effect on certain soluble pollutants or colloidal substances is not good. In addition, the physical method equipment is complex and the operation and maintenance costs are high. Biodegradation, as an environmentally friendly and efficient sewage treatment technology, has gradually received widespread attention. It uses microorganisms or biological enzymes to degrade organic matter in sewage and convert it into harmless or low-toxic substances. It has the advantages of efficient degradation of organic matter, no secondary pollution, energy saving and environmental protection.

[0004] Although biodegradation has many advantages, single biological enzymes often have problems such as low degradation efficiency and limited scope of application in practical applications. On the one hand, the types and concentrations of pollutants in different water qualities vary greatly, and a single biological enzyme is difficult to deal with all types of pollutants. On the other hand, the activity of biological enzymes is greatly affected by environmental factors such as temperature and pH, and their stability is poor, making it difficult to ensure sustained and efficient degradation effects.

[0005] In order to overcome the limitations of a single biological enzyme and improve the efficiency of water degradation, the present invention proposes a water purification biological enzyme composite reagent. This composite reagent can work synergistically to improve degradation efficiency and expand the scope of application by scientifically matching multiple biological enzymes and auxiliary components. At the same time, by optimizing the preparation process, the stability of the biological enzyme is improved to ensure that it can continuously and efficiently exert its degradation effect in practical applications. Summary of the Invention

[0006] The invention provides a water purification bioenzyme reagent and a preparation method thereof.

[0007] The technical solution adopted in the present invention is:

[0008] A water purification bioenzyme reagent and a preparation method thereof, wherein the reagent is composed of the following components in parts by weight:

[0009] Biological enzymes 34%-42%;

[0010] Microorganisms 5%-11%;

[0011] Deionized water 30%-40%;

[0012] Activated carbon 15%-20%;

[0013] Orange powder 2%-5%;

[0014] Flocculant 5%-7%;

[0015] The preparation method of the orange powder comprises: pre-treating orange peels, drying at 80° C., grinding and screening powder with a mesh size of less than 200, mixing 0.5 g of the powder with 100 mL of acetic acid solution, hydroheating at 180° C. for 6-8 hours, centrifuging and refrigerating to obtain the orange powder;

[0016] The composition and weight proportions of the flocculant are: 10-15 parts of polyacrylamide, 15-30 parts of aluminum sulfate, and 5-10 parts of delignified poplar wood powder.

[0017] Furthermore, the composition and weight proportions of the biological enzymes are: 6-7 parts of amylase, 8-9 parts of lipase, 8-12 parts of protease, 6-8 parts of cellulase, and 5-7 parts of catalase.

[0018] Furthermore, the microorganism includes any one of Bacillus subtilis, Bacillus licheniformis, and yeast, or a combination of at least two of them.

[0019] Furthermore, the activity of the biological enzyme is greater than 1000 U / g, and the concentration of the biological enzyme is 1-10 g / L.

[0020] Furthermore, the viable count of the microorganisms is 1×108-5×108 CFU / g.

[0021] Furthermore, in the preparation method of the orange powder, the pretreatment process uses anhydrous ethanol and deionized water for multiple ultrasonic cleanings.

[0022] Furthermore, 25-30 mL of water purification enzyme reagent is dissolved in every cubic meter of water.

[0023] Furthermore, a method for preparing a water purification enzyme reagent comprises the following steps:

[0024] (1) placing microorganisms in a culture medium according to weight ratio;

[0025] (2) adding deionized water to obtain a microbial preparation;

[0026] (3) Add activated carbon, orange powder and flocculant to the microbial preparation according to weight fraction and mix well.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] 1. A variety of biological enzymes can decompose organic matter, oils and other pollutants in water, converting them into harmless or low-toxic substances, reducing secondary pollution to the environment; adding microorganisms to water purification biological enzyme reagents can further enhance the degradation effect of biological enzymes. At the same time, the microorganisms themselves can consume nutrients in the water during the reproduction process, reducing the risk of eutrophication of water bodies; activated carbon has a rich porous structure and acts as a physical adsorbent to complement biological enzymes and microorganisms, improving the overall purification effect; in the water purification process, flocculants condense tiny particles, colloids, etc. in the water into larger particles, which are convenient for subsequent filtration or precipitation treatment. At the same time, flocculants can also accelerate the degradation process of pollutants in water by biological enzymes and microorganisms.

[0029] 2. The flocculant is composed of polyacrylamide, aluminum sulfate and delignified poplar wood powder. The three work synergistically and have a good flocculation effect. At the same time, delignified poplar wood powder has rich pore structure and cellulose, which can improve water quality during the water purification process.

[0030] 3. Orange powder acts as a natural adsorbent, working together with activated carbon to improve the physical adsorption capacity of biological enzyme reagents. Orange powder also contains a certain amount of citric acid and other substances, which can promote the dissolution and balance of minerals in water and improve the pH value of water. DETAILED DESCRIPTION

[0031] The present invention will be described in detail below with reference to specific embodiments. The following embodiments will help those skilled in the art to further understand the present invention, but are not intended to limit the present invention in any form. It should be noted that, for those skilled in the art, several variations and improvements can be made without departing from the scope of the present invention. These all fall within the scope of protection of the present invention.

[0032] Example 1

[0033] A method for preparing a water purification bioenzyme reagent comprises the following steps: placing microorganisms in a culture medium according to weight ratio for cultivation; adding deionized water to obtain a microbial preparation; and adding activated carbon, orange powder, and a flocculant to the microbial preparation according to weight fractions, and mixing them uniformly.

[0034] A water purification bioenzyme reagent and a preparation method thereof, the reagent is composed of the following components in parts by weight: 40% bioenzyme; 8% microorganism; 35% deionized water; 18% activated carbon; 2% orange powder; 6% flocculant;

[0035] The flocculant has the following raw material components: 12 parts of polyacrylamide, 20 parts of aluminum sulfate, and 7 parts of delignified poplar wood powder.

[0036] The raw material components of the biological enzyme are as follows: 6 parts of amylase, 8 parts of lipase, 10 parts of protease, 7 parts of cellulase, and 6 parts of catalase; the activity of the biological enzyme is 2000 U / g, and the concentration of the biological enzyme is 2 g / L.

[0037] The microorganism is Bacillus subtilis, and the viable cell count is 2×10 8 CFU / g.

[0038] The preparation method of the orange powder comprises the following steps: ultrasonically cleaning orange peels with anhydrous ethanol and deionized water for multiple times, drying at 80° C., grinding and screening powders with a mesh size of less than 200, mixing 0.5 g of the powder with 100 mL of acetic acid solution, hydroheating at 180° C. for 7 h, centrifugally filtering, and cold-drying to obtain the orange powder.

[0039] Dissolve 30mL of water purification enzyme reagent in every cubic meter of sewage.

[0040] Example 2

[0041] A method for preparing a water purification bioenzyme reagent comprises the following steps: placing microorganisms in a culture medium according to weight ratio for cultivation; adding deionized water to obtain a microbial preparation; and adding activated carbon, orange powder, and a flocculant to the microbial preparation according to weight fractions, and mixing them uniformly.

[0042] A water purification bioenzyme reagent and a preparation method thereof, the reagent is composed of the following components in parts by weight: 40% bioenzyme; 8% microorganism; 35% deionized water; 18% activated carbon; 4% orange powder; 6% flocculant;

[0043] The flocculant has the following raw material components: 12 parts of polyacrylamide, 20 parts of aluminum sulfate, and 7 parts of delignified poplar wood powder.

[0044] The raw material components of the biological enzyme are as follows: 6 parts of amylase, 8 parts of lipase, 10 parts of protease, 7 parts of cellulase, and 6 parts of catalase; the activity of the biological enzyme is 3000 U / g, and the concentration of the biological enzyme is 5 g / L.

[0045] The microorganism is Bacillus subtilis, and the viable cell count is 3×10 8 CFU / g.

[0046] The preparation method of the orange powder comprises the following steps: ultrasonically cleaning orange peels with anhydrous ethanol and deionized water for multiple times, drying at 80° C., grinding and screening powders with a mesh size of less than 200, mixing 0.5 g of the powder with 100 mL of acetic acid solution, hydroheating at 180° C. for 7 h, centrifugally filtering, and cold-drying to obtain the orange powder.

[0047] Dissolve 30mL of water purification enzyme reagent in every cubic meter of sewage.

[0048] Example 3

[0049] A method for preparing a water purification bioenzyme reagent comprises the following steps: placing microorganisms in a culture medium according to weight ratio for cultivation; adding deionized water to obtain a microbial preparation; and adding activated carbon, orange powder, and a flocculant to the microbial preparation according to weight fractions, and mixing them uniformly.

[0050] A water purification bioenzyme reagent and a preparation method thereof, the reagent is composed of the following components in parts by weight: 42% bioenzyme; 8% microorganism; 35% deionized water; 18% activated carbon; 5% orange powder; 6% flocculant;

[0051] The flocculant has the following raw material components: 12 parts of polyacrylamide, 20 parts of aluminum sulfate, and 7 parts of delignified poplar wood powder.

[0052] The raw material components of the biological enzyme are as follows: 6 parts of amylase, 8 parts of lipase, 10 parts of protease, 7 parts of cellulase, and 6 parts of catalase; the activity of the biological enzyme is 5000 U / g, and the concentration of the biological enzyme is 8 g / L.

[0053] The microorganism is Bacillus subtilis, and the viable cell count is 4×10 8 CFU / g.

[0054] The preparation method of the orange powder comprises the following steps: ultrasonically cleaning orange peels with anhydrous ethanol and deionized water for multiple times, drying at 80° C., grinding and screening powders with a mesh size of less than 200, mixing 0.5 g of the powder with 100 mL of acetic acid solution, hydroheating at 180° C. for 7 h, centrifugally filtering, and cold-drying to obtain the orange powder.

[0055] Dissolve 30mL of water purification enzyme reagent in every cubic meter of sewage.

[0056] Comparative Example 1

[0057] A preparation method for a water purification bioenzyme reagent comprises the following steps: placing microorganisms in a culture medium according to weight ratio for cultivation; adding deionized water to obtain a microbial preparation; and adding activated carbon and a flocculant to the microbial preparation according to weight fractions, and mixing them uniformly.

[0058] A water purification bioenzyme reagent and a preparation method thereof, wherein the reagent is composed of the following components in parts by weight: 40% bioenzyme; 8% microorganism; 35% deionized water; 19% activated carbon; 7% flocculant;

[0059] The flocculant has the following raw material components: 12 parts of polyacrylamide, 20 parts of aluminum sulfate, and 7 parts of delignified poplar wood powder.

[0060] The raw material components of the biological enzyme are as follows: 6 parts of amylase, 8 parts of lipase, 10 parts of protease, 7 parts of cellulase, and 6 parts of catalase; the activity of the biological enzyme is 1000 U / g, and the concentration of the biological enzyme is 2 g / L.

[0061] The microorganism is Bacillus subtilis, and the viable cell count is 2×10 8 CFU / g.

[0062] Dissolve 30mL of water purification enzyme reagent in every cubic meter of sewage.

[0063] Comparative Example 2

[0064] A preparation method for a water purification bioenzyme reagent comprises the following steps: placing microorganisms in a culture medium according to weight ratio for cultivation; adding deionized water to obtain a microbial preparation; and adding activated carbon and a flocculant to the microbial preparation according to weight fractions, and mixing them uniformly.

[0065] A water purification bioenzyme reagent and a preparation method thereof, the reagent is composed of the following components in parts by weight: 40% bioenzyme; 8% microorganism; 35% deionized water; 18% activated carbon; 3% orange powder; 6% flocculant;

[0066] The flocculant has the following raw material components: 12 parts of polyacrylamide and 20 parts of aluminum sulfate.

[0067] The raw material components of the biological enzyme are as follows: 6 parts of amylase, 8 parts of lipase, 10 parts of protease, 7 parts of cellulase, and 6 parts of catalase; the activity of the biological enzyme is 1000 U / g, and the concentration of the biological enzyme is 2 g / L.

[0068] The microorganism is Bacillus subtilis, and the viable cell count is 2×10 8 CFU / g.

[0069] The preparation method of the orange powder comprises the following steps: ultrasonically cleaning orange peels with anhydrous ethanol and deionized water for multiple times, drying at 80° C., grinding and screening powders with a mesh size of less than 200, mixing 0.5 g of the powder with 100 mL of acetic acid solution, hydroheating at 180° C. for 7 h, centrifugally filtering, and cold-drying to obtain the orange powder.

[0070] Dissolve 30mL of water purification enzyme reagent in every cubic meter of sewage.

[0071] Take equal volumes of sewage, add the water purification enzyme reagents in Examples 1-3 and Comparative Examples 1-2 respectively, stir thoroughly for 6 hours, let stand for 24 hours, and then perform water quality testing. The specific data are shown in Table 1.

[0072] Table 1

[0073] COD (mg / L) <![CDATA[BOD5(mg / L)]]> Total nitrogen (mg / L) Total phosphorus (mg / L) Water quality indicators 2560 1526 52 48 Example 1 420.8 189.6 4.2 3.7 Example 2 397.5 216.0 2.7 4.2 Example 3 462.3 176.5 4.2 2.7 Comparative Example 1 723.0 328.7 18.7 10.8 Comparative Example 2 645.2 318.5 20.0 10.7

Claims

1. A water purification bioenzyme reagent and a preparation method thereof, characterized in that: The reagent is composed of the following components in parts by weight: Biological enzyme 34%-42%; Microorganisms 5%-11%; Deionized water 30%-40%; Activated carbon 15%-20%; Orange powder 2%-5%; Flocculant 5%-7%; The preparation method of the orange powder is as follows: pre-treating orange peels, drying at 80° C., grinding and screening powders with a mesh size of less than 200, mixing 0.5 g of the powder with 100 mL of acetic acid solution, hydroheating at 180° C. for 6-8 hours, centrifugally filtering and cold drying to obtain orange powder; The composition and weight proportions of the flocculant are: 10-15 of polyacrylamide, 15-30 of aluminum sulfate, and 5-10 of delignified poplar wood powder.

2. A water purification bioenzyme reagent and a preparation method thereof according to claim 1, characterized in that: The composition and weight proportions of the biological enzymes are: 6-7 parts of amylase, 8-9 parts of lipase, 8-12 parts of protease, 6-8 parts of cellulase, and 5-7 parts of catalase.

3. A water purification bioenzyme reagent and a preparation method thereof according to claim 1, characterized in that: The microorganisms include any one of Bacillus subtilis, Bacillus licheniformis and yeast, or a combination of at least two of them.

4. A water purification bioenzyme reagent and a preparation method thereof according to claim 2, characterized in that: The activity of the biological enzyme is greater than 1000 U / g, and the concentration of the biological enzyme is 1-10 g / L.

5. A water purification bioenzyme reagent and a preparation method thereof according to claim 3, characterized in that: The viable count of the microorganism is 1×10 8 -5×10 8 CFU / g.

6. A water purification bioenzyme reagent and a preparation method thereof according to claim 1, characterized in that: In the preparation method of orange powder, anhydrous ethanol and deionized water are used for multiple ultrasonic cleaning in the pretreatment process.

7. A water purification bioenzyme reagent and a preparation method thereof according to claim 1, characterized in that: Dissolve 25-30mL of water purification enzyme reagent per cubic meter of water.

8. A method for preparing a water purification bioenzyme reagent as claimed in claim 1, characterized in that: The steps are as follows: (1) placing microorganisms in a culture medium according to a weight ratio for cultivation; (2) adding deionized water to obtain a microbial preparation; (3) Add activated carbon, orange powder and flocculant to the microbial preparation according to weight fraction and mix well.