Enzyme-controlled synergist and enzyme-controlled fertilizer composition and application thereof in agricultural planting

Abstract

The invention relates to the field of agricultural planting, and discloses an enzyme-controlled synergist and enzyme-controlled fertilizer composition and application thereof in agricultural planting, the composition comprises the following components by mass: the enzyme-controlled synergist comprising 4-7 parts of phosphatase; 3 to 5 parts of nitrogenase; 2 to 4 parts of protease; 1 to 3 parts of nitrate reductase; 3-5 parts of a colloid protective agent; 2-5 parts of organic acid; the enzyme-controlled fertilizer comprises the following components in parts by weight: 10-15 parts of polyethyleneimine modified nano silicon; 5 to 10 parts of a temperature response type polymer; 5 to 10 parts of a nano carbon material; and 2-5 parts of a colloidal compound. The enzyme-controlled synergist and the enzyme-controlled fertilizer are mixed, unavailable phosphorus and nitrogen in soil are converted into absorbable forms by enzyme catalysis, and the effectiveness of fertilizer nutrients is improved. Phosphatase releases phosphorus, and nitrate reductase promotes nitrogen conversion, so that the fertilizer can act on multiple nutrients at the same time, the problem of incomplete conversion is solved, the nutrient utilization rate is increased, and fertilizer waste is reduced.

Description

Technical Field

[0001] The present invention relates to the field of agricultural planting, in particular to an enzyme-controlled synergist and an enzyme-controlled fertilizer composition and applications thereof in agricultural planting. Background Art

[0003] In traditional fertilizer technology, the conversion of phosphorus and nitrogen mainly relies on the natural action of soil microorganisms. However, this natural conversion process is slow and incomplete, resulting in a large amount of unavailable phosphorus and nitrogen sources in the soil. Most of the nutrient forms such as phosphate and nitrate nitrogen are difficult to be directly absorbed by crops, which seriously affects the utilization efficiency of fertilizers. In this context, the prior art usually uses a single enzyme fertilizer, such as phosphatase fertilizer, nitrate reductase fertilizer, etc., to improve the conversion rate of phosphorus and nitrogen. However, these technologies are often limited to the conversion of a certain type of nutrients and cannot effectively solve the problem of incomplete conversion of multiple nutrients in the soil.

[0004] For example, traditional phosphatase fertilizers can only release phosphorus in the soil, while the conversion of nitrogen depends on other different enzymes. These single enzyme fertilizers often face the following problems in practical applications: on the one hand, the scope of action of single enzyme fertilizers is limited and cannot meet the needs of crops for multiple nutrients during growth; on the other hand, since the activity of various enzymes is greatly affected by the soil environment (such as pH, temperature, etc.), the effect of single enzyme fertilizers will also fluctuate greatly, resulting in unstable fertilizer effects and difficulty in achieving the expected nutrient conversion effect.

[0005] In addition, the existing fertilizer application method does not fully consider the synergistic effect of nutrient conversion. Although adding only one enzyme to the fertilizer can promote the conversion of a certain type of nutrients, it cannot effectively solve the problem of synchronous conversion of multiple nutrients such as phosphorus and nitrogen in the soil. The limitations of this technical solution result in a large number of nutrients in the fertilizer being unable to fully play their role, increasing the cost of fertilizer use and also bringing a burden to the environment. Summary of the invention

[0006] In view of the deficiencies of the prior art, the present invention provides an enzyme-controlled synergist and an enzyme-controlled fertilizer composition and their application in agricultural planting, which solves the problems in the prior art of incomplete nutrient conversion, low fertilizer utilization rate and nutrient waste caused by the application of a single enzyme.

[0007] To achieve the above objectives, the present invention is implemented by the following technical scheme: the enzyme-controlled synergist and enzyme-controlled fertilizer composition comprises the following mass-counted components:

[0008] Enzyme control enhancers, including:

[0009] Phosphatase, 4-7 copies;

[0010] Since phosphatase can decompose phosphorus sources into inorganic phosphorus through hydrolysis reactions, and inorganic phosphorus can be effectively absorbed by plant roots, its activity is affected by temperature, pH value and soil conditions. By introducing phosphatase into the fertilizer system, it can promote the rapid unlocking of phosphorus in the soil and improve the efficiency of plant phosphorus absorption.

[0011] Nitrogenase, 3-5 parts;

[0012] Organic nitrogen compounds are converted into nitrates that can be absorbed by plants through nitrogenase. Nitrate is an important nutrient for plant growth. The activity of nitrogenase is affected by environmental factors such as soil pH and temperature. By introducing nitrogenase, the conversion of soil nitrogen can be accelerated and the effective utilization rate of nitrogen in fertilizers can be improved.

[0013] Protease, 2-4 parts;

[0014] Protease can hydrolyze protein and break down complex organic matter into simple amino acids for plant absorption, helping to increase the effectiveness of nitrogen in the soil and improve soil fertility and health.

[0015] Nitrate reductase, 1-3 parts;

[0016] Nitrate reductase can reduce nitrate to ammonia to promote nitrogen absorption by plants. Under suitable conditions, nitrate reductase can significantly improve the utilization efficiency of nitrogen in the soil and reduce the loss of nitrogen sources.

[0017] Colloidal protective agent, 3 to 5 parts;

[0018] Colloidal protective agents can form a protective film to increase the stability of enzymes, preventing them from losing their activity due to environmental changes. They can also adjust the distribution of nutrients in fertilizers to avoid excessive release or loss of nutrients.

[0019] Organic acid, 2-5 parts;

[0020] Organic acids can lower the pH of the soil, thereby promoting the solubility and absorption of certain nutrients (such as iron, phosphorus, calcium, etc.). They can also help soil microbial activity and promote soil health.

[0021] Enzyme-controlled fertilizers, including:

[0022] Polyethyleneimine modified nano-silicon, 10-15 parts;

[0023] By modifying the surface amino groups of nano-silicon, its affinity with fertilizers and enzymes can be increased, the stability of the carrier is enhanced, and the slow release of fertilizers and enzymes is facilitated. The high specific surface area of ​​nano-silicon can provide more adsorption and desorption space for fertilizers, ensuring the continuous release of nutrients.

[0024] Temperature responsive polymer, 5 to 10 parts;

[0025] At high temperatures, the solubility of the polymer increases, promoting the release of fertilizers and enzymes, while at low temperatures the release rate is reduced. This intelligent regulation ensures that fertilizers and enzymes can adapt to the needs of different growth stages.

[0026] Nano carbon material, 5 to 10 parts;

[0027] Because nanocarbon materials have extremely high specific surface area and excellent conductivity, they can enhance the affinity of fertilizers to soil, improve their permeability, and improve the distribution of fertilizers in the soil. Through their unique surface structure, they can also improve the bioavailability of fertilizers and enzymes.

[0028] colloidal compound, 2 to 5 parts;

[0029] Colloidal compounds can form a colloidal network structure to control the release rate of nutrients in fertilizers and avoid nutrient loss. They can also help distribute fertilizers evenly in the soil through their high water absorption and promote balanced absorption by crops.

[0030] Wherein, the ratio of the enzyme-controlled synergist to the enzyme-controlled fertilizer is 1:5 to 1:10.

[0031] Preferably, the phosphatase is obtained by fermentation of a microorganism, wherein the microorganism comprises one of Bacillus subtilis, Bacillus licheniformis or Penicillium.

[0032] Preferably, the nitrogenase is a synthase, including one or more of nitrate reductase or amino acid transaminase.

[0033] Preferably, the polyethyleneimine-modified nano-silicon is based on silicon dioxide nanoparticles, and the surface is chemically modified by amino groups.

[0034] Preferably, the temperature-responsive polymer is selected from one of polyacrylic acid and polyvinyl alcohol.

[0035] Preferably, the nano-carbon material is one of carbon nanotubes or graphene.

[0036] Preferably, the colloidal protective agent includes one of polyacrylamide or carboxymethyl cellulose.

[0037] Preferably, the gelatinous compound comprises one or more of polyvinyl alcohol, polyacrylate, gelatin, gum arabic or agar.

[0038] Preferably, the composition is applied by one or more of spraying, broadcasting or burying in the soil.

[0039] Preferably, the composition is used in agricultural planting.

[0040] The invention provides an enzyme-controlled synergist and an enzyme-controlled fertilizer composition and application thereof in agricultural planting.

[0041] It has the following beneficial effects:

[0042] 1. The present invention uses an enzyme-controlled synergist mixed with an enzyme-controlled fertilizer, and through an enzyme-catalyzed bioconversion process, converts unavailable phosphorus and nitrogen sources in the soil into a form that can be directly absorbed by crops, thereby improving the effectiveness of nutrients in the fertilizer. Phosphatase releases absorbable phosphorus through a hydrolysis reaction, and nitrate reductase promotes the conversion of nitrogen. Compared with fertilizers using a single enzyme in the prior art, the present invention can act on multiple nutrients at the same time, solving the problem of incomplete nutrient conversion in the soil, improving nutrient utilization, and reducing fertilizer waste.

[0043] 2. The present invention uses a temperature-responsive polymer. When the temperature changes, the structure of the polymer will change, thereby adjusting the release rate of the fertilizer and the synergist. At low temperatures, the polymer maintains a low solubility and the release rate is slow; at high temperatures, the solubility of the polymer is enhanced and the release rate is accelerated, ensuring that crops are evenly supplied at different growth stages. Different from the fixed release mode of traditional fertilizers, the present invention avoids the problem of excessive or insufficient nutrients caused by climate change, effectively reduces nutrient loss, avoids fertilizer waste and soil pollution, and thus optimizes fertilizer use efficiency.

[0044] 3. The present invention adopts polyethyleneimine-modified nano-silicon and nano-carbon materials. The polyethyleneimine-modified nano-silicon is used as a carrier to provide more adsorption sites through its high specific surface area. The nano-carbon material promotes the dispersion and stability of fertilizers and enzymes through its excellent conductivity and strong adsorption capacity, which can not only improve the distribution of fertilizers, but also delay the release of fertilizers by increasing the specific surface area and optimizing the release characteristics, thereby reducing the frequency of fertilization. Compared with non-nanomaterials commonly found in traditional fertilizers, the nano-composite material of the present invention can increase the dissolution rate and availability of fertilizers, further enhance the effect of fertilizers, and optimize the interaction between fertilizers and soil.

[0045] 4. The present invention combines enzyme-controlled synergists with fertilizers and adds colloidal compounds to make the release of fertilizer nutrients more stable, avoiding premature loss of nutrients due to strong water solubility. Colloidal compounds can form a network structure with the active ingredients in the fertilizer when applied, slowly releasing nutrients, and the structure has high water absorption. After application, it can stably exist in the soil and effectively release nutrients slowly, avoiding the problem of rapid loss of nutrients in water-soluble fertilizers due to water. Compared with traditional fertilizers, the present invention effectively delays the release process of nutrients through colloidal compounds, ensuring that crops can continue to obtain the required nutrients at all stages of growth, especially in drought or water-deficient environments, significantly improving the stress resistance and growth stability of crops. DETAILED DESCRIPTION

[0046] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0047] In order to better understand the present invention, the above contents are described in detail below in conjunction with specific embodiments.

[0048] Embodiment 1:

[0049] Components:

[0050] Phosphatase: 4 parts

[0051] Nitrogenase: 3 parts

[0052] Protease: 2 parts

[0053] Nitrate reductase: 1 part

[0054] Colloidal protective agent (polyacrylamide): 3.5 parts Organic acid (humic acid): 3 parts

[0055] Polyethyleneimine modified nano silicon: 10 parts

[0056] Temperature responsive polymer (polyacrylic acid): 5 parts Nanocarbon material (graphene): 5 parts Colloidal compound (polyvinyl alcohol): 3 parts

[0057] Proportion:

[0058] Ratio of enzyme-controlled synergist to enzyme-controlled fertilizer: 1:7 Example 2:

[0059] Components:

[0060] Phosphatase: 5 parts

[0061] Nitrate reductase: 4 parts

[0062] Nitrogenase: 2 parts

[0063] Protease: 2 parts

[0064] Colloidal protective agent (polyacrylamide): 4 parts

[0065] Organic acid (humic acid): 3 parts

[0066] Polyethyleneimine modified nano silicon: 12 parts

[0067] Polyvinyl alcohol: 6 parts

[0068] Nanocarbon material (carbon nanotube): 5 parts Colloidal compound (gelatin): 3 parts

[0069] Proportion:

[0070] Ratio of enzyme-controlled synergist to enzyme-controlled fertilizer: 1:8 Example 3:

[0071] Components:

[0072] Phosphatase: 6 parts

[0073] Nitrogenase: 3 parts

[0074] Protease: 2 parts

[0075] Nitrate reductase: 2 parts

[0076] Colloidal protective agent (polyacrylamide): 4 parts Organic acid (humic acid): 4 parts

[0077] Polyethyleneimine modified nano silicon: 13 parts

[0078] Polyacrylic acid: 6 parts

[0079] Nanocarbon material (graphene): 6 parts Colloidal compound (polyvinyl alcohol): 4 parts Ratio:

[0080] Ratio of enzyme-controlled synergist to enzyme-controlled fertilizer: 1:9 Example 4:

[0081] Components:

[0082] Phosphatase: 4 parts

[0083] Nitrogenase: 4 parts

[0084] Protease: 2 parts

[0085] Nitrate reductase: 2 parts

[0086] Colloidal protective agent (polyacrylamide): 3 parts Organic acid (humic acid): 3 parts

[0087] Polyethyleneimine modified nano silicon: 11 parts

[0088] Polyvinyl alcohol: 5 parts

[0089] Nanocarbon material (graphene): 5 parts Colloidal compound (gelatin): 4 parts

[0090] Proportion:

[0091] Ratio of enzyme-controlled synergist to enzyme-controlled fertilizer: 1:6 Example 5:

[0092] Components:

[0093] Phosphatase: 5 parts

[0094] Nitrogenase: 3 parts

[0095] Protease: 2 parts

[0096] Nitrate reductase: 1 part

[0097] Colloidal protective agent (polyacrylamide): 3 parts Organic acid (humic acid): 4 parts

[0098] Polyethyleneimine modified nano silicon: 10 parts

[0099] Polyacrylic acid: 5 parts

[0100] Nanocarbon material (graphene): 4 parts Colloidal compound (polyvinyl alcohol): 3 parts Ratio:

[0101] Ratio of enzyme-controlled synergist to enzyme-controlled fertilizer: 1:7 Example 6:

[0102] Components:

[0103] Phosphatase: 6 parts

[0104] Nitrogenase: 2 parts

[0105] Protease: 2 parts

[0106] Nitrate reductase: 1 part

[0107] Colloidal protective agent (polyacrylamide): 3 parts

[0108] Organic acid (humic acid): 4 parts

[0109] Polyethyleneimine modified nano silicon: 11 parts

[0110] Polyacrylic acid: 5 parts

[0111] Nanocarbon materials (carbon nanotubes): 5 parts

[0112] Colloidal compound (polyvinyl alcohol): 4 parts

[0113] Proportion:

[0114] Ratio of enzyme-controlled synergist to enzyme-controlled fertilizer: 1:9

[0115] Comparative Example 1 (corresponding to Example 1): Fertilizer release comparison component without using temperature responsive polymer:

[0116] Phosphatase: 4 parts

[0117] Nitrogenase: 3 parts

[0118] Protease: 2 parts

[0119] Nitrate reductase: 1 part

[0120] Colloidal protective agent (polyacrylamide): 3.5 parts

[0121] Organic acid (humic acid): 3 parts

[0122] Polyethyleneimine modified nano silicon: 10 parts

[0123] Temperature responsive polymer (polyacrylic acid): 0 parts (not added)

[0124] Nanocarbon material (graphene): 5 parts

[0125] Colloidal compound (polyvinyl alcohol): 3 parts

[0126] Comparative Example 2 (corresponding to Example 2): Fertilizer release rate comparison component without using colloidal compound:

[0127] Phosphatase: 5 parts

[0128] Nitrate reductase: 4 parts

[0129] Nitrogenase: 2 parts

[0130] Protease: 2 parts

[0131] Colloidal protective agent (polyacrylamide): 4 parts

[0132] Organic acid (humic acid): 3 parts

[0133] Polyethyleneimine modified nano silicon: 12 parts

[0134] Polyvinyl alcohol: 6 parts

[0135] Nanocarbon materials (carbon nanotubes): 5 parts

[0136] Gelatin: 0 (not used)

[0137] Comparative Example 3 (corresponding to Example 3): Comparative components and proportions of fertilizer dispersibility without using nano-carbon materials:

[0138] Phosphatase: 6 parts

[0139] Nitrogenase: 3 parts

[0140] Nitrate reductase: 2 parts

[0141] Colloidal protective agent (polyacrylamide): 4 parts

[0142] Organic acid (humic acid): 4 parts

[0143] Polyethyleneimine modified nano silicon: 13 parts

[0144] Polyacrylic acid: 6 parts

[0145] Nanocarbon material (graphene): 0 parts (not used)

[0146] Colloidal compound (polyvinyl alcohol): 4 parts

[0147] Comparative Example 4 (corresponding to Example 4): Comparative Components of Fertilizer Effect without Enzyme Controlled Synergist:

[0148] Phosphatase: 0 (not used)

[0149] Nitrogenase: 0 (unused)

[0150] Protease: 0 parts (not used)

[0151] Nitrate reductase: 0 parts (not used)

[0152] Colloidal protective agent (polyacrylamide): 4 parts

[0153] Organic acid (humic acid): 4 parts

[0154] Polyethyleneimine modified nano silicon: 12 parts

[0155] Polyacrylic acid: 6 parts

[0156] Nanocarbon material (graphene): 5 parts

[0157] Gelatin: 3 parts

[0158] Comparative Example 5 (corresponding to Example 5): Comparison between conventional fertilizers and those without using nanocarbon materials

[0159] Components:

[0160] Phosphatase: 5 parts

[0161] Nitrogenase: 3 parts

[0162] Protease: 2 parts

[0163] Nitrate reductase: 1 part

[0164] Colloidal protective agent (polyacrylamide): 3 parts

[0165] Organic acid (humic acid): 4 parts

[0166] Polyethyleneimine modified nano silicon: 10 parts

[0167] Polyacrylic acid: 5 parts

[0168] Nanocarbon material (graphene): 0 parts (unused)

[0169] Colloidal compound (polyvinyl alcohol): 3 parts

[0170] Comparative Example 6 (corresponding to Example 6): No temperature-responsive polymer and colloidal compound are used for comparison components:

[0171] Phosphatase: 5 parts

[0172] Nitrogenase: 4 parts

[0173] Protease: 2 parts

[0174] Nitrate reductase: 2 parts

[0175] Colloidal protective agent (polyacrylamide): 4 parts

[0176] Organic acid (humic acid): 4 parts

[0177] Polyethyleneimine modified nano silicon: 11 parts

[0178] Polyvinyl alcohol: 5 parts

[0179] Nanocarbon material (graphene): 5 parts

[0180] Gelatin: 0 (not used)

[0181] Temperature responsive polymer (polyacrylic acid): 0 parts (not used)

[0182] Experiment 1: Effect of temperature-responsive polymer on fertilizer release rate

[0183] Purpose:

[0184] This experiment aims to verify the regulatory effect of temperature-responsive polymers (such as polyacrylic acid) on the fertilizer release rate under different temperature conditions. By comparing with traditional fertilizers, it is evaluated whether temperature-responsive polymers can regulate fertilizer release according to changes in ambient temperature, thereby improving fertilizer use efficiency and reducing nutrient loss.

[0185] sample:

[0186] Example 1: Using a temperature-responsive polymer (polyacrylic acid)

[0187] Comparative Example 1: No temperature responsive polymer used

[0188] Experimental field setting:

[0189] Number and selection of experimental fields:

[0190] Four experimental plots were selected, each with an area of ​​10 square meters (2mx5m), ensuring that the soil type was uniform and there were no significant differences in drainage or ventilation between the experimental plots. Each experimental plot was divided into different temperature groups.

[0191] Experimental field distribution:

[0192] Experimental field 1 (20°C group, Example 1): The fertilizer of Example 1 was applied and the temperature was controlled at 20°C.

[0193] Experimental field 2 (20°C group, comparative example 1): the fertilizer of comparative example 1 was applied and the temperature was controlled at 20°C.

[0194] Experimental field 3 (30°C group, Example 1): The fertilizer of Example 1 was applied and the temperature was controlled at 30°C.

[0195] Experimental field 4 (30°C group, comparative example 1): the fertilizer of comparative example 1 was applied and the temperature was controlled at 30°C.

[0196] Temperature Control:

[0197] A greenhouse was used to maintain the temperature of each test field. The temperature in the greenhouse was adjusted by a temperature control system and set at 20℃ and 30℃ respectively. In the 30℃ test field, heating equipment was used to ensure stable temperature. The 20℃ group maintained a lower temperature by using a sunshade net.

[0198] Steps:

[0199] Soil preparation:

[0200] Each experimental field was subjected to basic treatment to remove weeds, stones and other debris to ensure that the soil was flat and free of large debris.

[0201] Conduct a preliminary soil analysis to ensure that the soil pH, moisture, and nutrient content are similar in each experimental plot.

[0202] Fertilizer application:

[0203] Each experimental plot was fertilized with 60 grams of fertilizer per square meter. After the fertilizer was applied, the soil was evenly raked with a rake to ensure that the fertilizer was fully mixed with the soil.

[0204] Data collection:

[0205] Soil samples were taken every 7 days to measure the release rate of phosphorus and nitrogen in the soil. After each sampling, the sample data was recorded and sent to the laboratory for nutrient analysis.

[0206] Experimental period:

[0207] The experimental period of each test field is 30 days. By comparing the release of fertilizers at different temperatures, the effect of temperature-responsive polymers on the fertilizer release rate is analyzed.

[0208] Table 1: Fertilizer release rate test data

[0209]

[0210]

[0211] Experimental Summary

[0212] From the experimental results, the temperature-responsive polymer fertilizer in Example 1 showed a significant release rate regulation effect under different temperature conditions. Especially at 40°C, the phosphorus and nitrogen release of the fertilizer increased significantly, and the release rate was significantly accelerated, which shows that the polyacrylic acid polymer can effectively promote the release of the fertilizer. The fertilizer in Comparative Example 1 showed a fixed release rate at all temperatures, could not be adaptively adjusted according to temperature changes, and showed a relatively simple performance. The addition of temperature-responsive polymers enables the release of fertilizers to be adjusted according to temperature changes, ensuring that the needs of crops at different temperatures are met.

[0213] From a mechanistic perspective, the main mechanism of action of temperature-responsive polymers such as polyacrylic acid is to regulate the release of fertilizers by changing their solubility. At low temperatures, the solubility of the polymer is low, which slows down the release of fertilizers; at high temperatures, the solubility of the polymer increases, accelerating the release of fertilizers. This dynamic regulation mechanism enables fertilizers to provide the required nutrients according to crop needs, avoiding fertilizer waste and improving the crop's absorption efficiency of fertilizers.

[0214] The results of this experiment show the advantages of temperature-responsive polymers, especially in regulating the fertilizer release rate. Compared with traditional fertilizers, fertilizers using temperature-responsive polymers can more accurately control nutrient release, reduce nutrient loss, and improve fertilizer utilization efficiency, providing an effective solution for precise fertilization and reducing fertilizer waste.

[0215] Experiment 2: Effect of colloidal compounds on nutrient release from fertilizers

[0216] Purpose:

[0217] This experiment aims to evaluate the effect of colloidal compounds (such as gelatin, polyvinyl alcohol, etc.) on the nutrient release rate of fertilizers. By comparing with traditional fertilizers without colloidal compounds, the role of colloidal compounds in slow-release fertilizers is explored to further verify its effectiveness in improving fertilizer utilization and reducing nutrient loss.

[0218] sample:

[0219] Example 2: Using a gelatinous compound (such as gelatin or polyvinyl alcohol)

[0220] Comparative Example 2: No colloidal compound is used

[0221] Experimental field setting:

[0222] Number and selection of experimental fields:

[0223] Select 4 test plots, each with an area of ​​12 square meters (3mx4m). These test plots should have uniform soil types and environmental conditions to ensure the effectiveness of the experiment. Choose a suitable location, the soil should be free of serious impurities and have good aeration and drainage.

[0224] Experimental field distribution:

[0225] The four experimental plots were divided into two groups:

[0226] Group 1 (Example 2): Fertilizer of Example 2 (containing colloidal compound) was applied

[0227] Group 2 (Comparative Example 2): Fertilizer of Comparative Example 2 (without colloidal compound) was applied

[0228] The two test fields in each group were set at different temperature conditions (20°C and 30°C) to ensure that the data could cover the effects of different ambient temperatures on fertilizer release.

[0229] Experimental field 1 (20°C, Example 2): Fertilizer of Example 2 was applied and the temperature was controlled at 20°C.

[0230] Experimental field 2 (20°C, comparative example 2): Fertilizer of comparative example 2 was applied and the temperature was controlled at 20°C.

[0231] Experimental field 3 (30°C, Example 2): Fertilizer of Example 2 was applied and the temperature was controlled at 30°C.

[0232] Experimental field 4 (30°C, comparative example 2): Fertilizer of comparative example 2 was applied and the temperature was controlled at 30°C.

[0233] Temperature Control:

[0234] Greenhouses were used to maintain temperatures between 20°C and 30°C to ensure temperature stability. The temperature of each experimental field was regulated by a heating and shading system to maintain a constant temperature.

[0235] Steps:

[0236] Soil preparation:

[0237] The soil of each experimental field was pretreated by removing weeds and debris and keeping the soil loose. The soil pH value should be controlled within the range of 6.5-7.0 and the humidity should be appropriate.

[0238] The nutrient content of each soil was checked using a soil tester to ensure that the initial soil conditions were consistent across all experimental plots.

[0239] Fertilizer application:

[0240] The fertilizer was applied to the soil at an application rate of 60 g per square meter. A colloidal compound was added to the fertilizer of Example 2, while the fertilizer of Comparative Example 2 did not contain any colloidal compound. After fertilization, a small rake was used to evenly rake the soil to ensure good contact between the fertilizer and the soil.

[0241] Data collection:

[0242] Samples were taken from the test fields every seven days to measure the concentrations of phosphorus and nitrogen in the soil. The experiment lasted for 30 days, and the effects of different fertilizers were analyzed by comparing the dissolution rates of the fertilizers.

[0243] After each sampling, soil samples were sent to the laboratory for analysis to ensure accurate determination of the fertilizer release rate.

[0244] Table 2: Fertilizer release rate test data

[0245]

[0246] Experimental Summary

[0247] The experimental results show that the fertilizer using the colloidal compound in Example 2 has certain advantages in release rate. In particular, under the environment of 30°C, the fertilizer release rate is significantly accelerated, especially in the early stage, the fertilizer of Example 2 shows a higher nutrient release efficiency. In contrast, the fertilizer release rate of Comparative Example 2 is relatively stable and the variation range is small. This shows that the colloidal compound can effectively adjust the release rate of the fertilizer and avoid premature loss or too fast dissolution of nutrients.

[0248] From a mechanistic point of view, the mechanism of action of colloidal compounds such as gelatin or polyvinyl alcohol is that they can form a relatively stable gel network structure in fertilizers. This structure can change its solubility when the temperature changes, thereby adjusting the dissolution rate of the fertilizer. At low temperatures, the solubility of colloidal compounds is low, slowing down the release of fertilizers; at high temperatures, the solubility of colloidal compounds increases, accelerating the release of fertilizers. This feature enables fertilizers to dynamically adjust nutrient release according to crop needs under different environmental conditions.

[0249] This experiment shows that colloidal compounds play a unique role in controlling fertilizer release. They can not only control the rate of nutrient release, but also adjust in time according to different environmental conditions. This provides a new idea for precise fertilization and reducing fertilizer waste. Especially under high temperature conditions, the use of colloidal compounds significantly improves the efficiency of nutrient release, providing effective technical support for precise fertilization in agricultural production.

[0250] Experiment 3: Effect of Nanocarbon Materials on Fertilizer Dispersion

[0251] Purpose:

[0252] This experiment aims to evaluate the effect of nanocarbon materials (such as graphene) on the dispersion and absorption efficiency of fertilizers. By comparing with traditional fertilizers without nanocarbon materials, the effect of nanocarbon materials in fertilizers is studied, especially the potential in promoting uniform distribution of fertilizers and improving nutrient absorption efficiency.

[0253] sample:

[0254] Example 3: Using nanocarbon materials (such as graphene)

[0255] Comparative Example 3: No use of nanocarbon material

[0256] Experimental field setting:

[0257] Number and selection of experimental fields:

[0258] Four test plots were selected for the experiment, each with an area of ​​12 square meters (3mx4m) to ensure uniform soil type and environmental conditions. The test plots should be selected in areas with unpolluted soil and good drainage.

[0259] Experimental field distribution:

[0260] The four experimental plots were divided into two groups, and different fertilizers were applied to each group:

[0261] Group 1 (Example 3): Application of the fertilizer of Example 3 (containing nanocarbon material)

[0262] Group 2 (Comparative Example 3): Fertilizer of Comparative Example 3 (without nano-carbon material) was applied

[0263] Different temperature conditions were set in the two experimental plots of each group:

[0264] Experimental field 1 (20°C, Example 3): Fertilizer of Example 3 was applied and the temperature was controlled at 20°C.

[0265] Experimental field 2 (20°C, comparative example 3): Fertilizer of comparative example 3 was applied and the temperature was controlled at 20°C.

[0266] Experimental field 3 (30°C, Example 3): Fertilizer of Example 3 was applied and the temperature was controlled at 30°C.

[0267] Experimental field 4 (30°C, comparative example 3): Fertilizer of comparative example 3 was applied and the temperature was controlled at 30°C.

[0268] Temperature Control:

[0269] A greenhouse was used to regulate the temperature of each experimental field. In the 30℃ group, heating equipment was used to control the temperature, while in the 20℃ group, the temperature was controlled by using a sunshade net to ensure that the temperature of each experimental field was stable.

[0270] Steps:

[0271] Soil preparation:

[0272] The soil of each experimental field was pretreated to remove weeds, stones and other debris to ensure that the soil was loose and free of large impurities.

[0273] Conduct soil testing to ensure that the initial conditions of each experimental field, such as soil pH, moisture, and nutrient content, are similar to reduce the impact of soil variables on experimental results.

[0274] Fertilizer application:

[0275] The application rate of each test field was 60 g of fertilizer per square meter. Nanocarbon materials were added to the fertilizer of Example 3, while nanocarbon materials were not included in the fertilizer of Comparative Example 3. After fertilization, a small rake was used to evenly rake the fertilizer into the soil to ensure good contact between the fertilizer and the soil.

[0276] Data collection:

[0277] Soil samples were taken every seven days to measure the concentrations of phosphorus and nitrogen in the soil. The experiment lasted for 30 days, monitoring the dissolution rate, dispersion and nutrient release of the fertilizer.

[0278] Experimental period:

[0279] The experimental period of each test field is 30 days. After each sampling, the data is recorded and sent to the laboratory for detailed analysis.

[0280] Table 3: Fertilizer dispersibility and release rate data

[0281]

[0282] Experimental Summary

[0283] The experimental data show that the fertilizer in Example 3 exhibits more significant dispersibility after adding the nanocarbon material. In particular, under high temperature environment (30°C), the phosphorus and nitrogen release rates of the fertilizer are significantly improved, and the uniform distribution of nutrients in the soil is significantly better than that in Comparative Example 3. This phenomenon shows that the nanocarbon material can effectively improve the dispersibility of the fertilizer, making it more evenly distributed in the soil, thereby improving the nutrient release efficiency of the fertilizer.

[0284] From a mechanistic point of view, the role of nanocarbon materials such as graphene is mainly reflected in enhancing the affinity between fertilizer and soil. Graphene has good conductivity and high surface area, which enables it to improve the dispersion of fertilizer particles in fertilizer. More uniform dispersion allows fertilizer to better penetrate into the soil, avoiding rapid loss caused by nutrient aggregation. In addition, the addition of graphene may also improve the contact efficiency between fertilizer and water, oxygen, etc. in the soil, thereby accelerating the release of nutrients.

[0285] In general, this experiment verified the advantages of nanocarbon materials in improving fertilizer dispersibility and nutrient release rate by comparing fertilizers containing nanocarbon materials with traditional fertilizers. The use of this material can not only improve the application effect of fertilizers, but also provide new technical support for precision fertilization and environmentally friendly agriculture.

[0286] Experiment 4: Comparison of the effect of fertilizer without enzyme-controlled synergist

[0287] Purpose:

[0288] This experiment aims to verify the effect of enzyme-controlled synergists (such as phosphatase, nitrogenase, etc.) on fertilizer nutrient release. By comparing fertilizers with enzyme-controlled synergists and fertilizers without enzyme-controlled synergists, the role of enzyme-controlled synergists in improving nutrient conversion efficiency and promoting effective fertilizer utilization is evaluated.

[0289] sample:

[0290] Example 4: Use of enzyme-controlled enhancers (such as phosphatase, nitrogenase)

[0291] Comparative Example 4: No enzyme-controlled enhancer

[0292] Experimental field setting:

[0293] Number and selection of experimental fields:

[0294] Four experimental plots were selected for the experiment, each with an area of ​​15 square meters (3mx5m) to ensure similar soil types and environmental conditions and avoid interference from external factors.

[0295] Experimental field distribution:

[0296] Group 1 (Example 4): Fertilizer of Example 4 (containing enzyme-controlled synergist) was applied

[0297] Group 2 (Comparative Example 4): Fertilizer of Comparative Example 4 (without enzyme-controlled synergist) was applied

[0298] The two test plots in each group were set at different temperature conditions (20℃ and 30℃):

[0299] Experimental field 1 (20°C, Example 4): Fertilizer of Example 4 was applied and the temperature was controlled at 20°C.

[0300] Experimental field 2 (20°C, comparative example 4): Fertilizer of comparative example 4 was applied and the temperature was controlled at 20°C.

[0301] Experimental field 3 (30°C, Example 4): Fertilizer of Example 4 was applied and the temperature was controlled at 30°C.

[0302] Experimental field 4 (30°C, comparative example 4): Fertilizer of comparative example 4 was applied and the temperature was controlled at 30°C.

[0303] Temperature Control:

[0304] The temperature of each test field was regulated using a greenhouse to maintain a stable temperature. The 30℃ group was controlled by a heating device, while the 20℃ group was cooled by a shade net.

[0305] Steps:

[0306] Soil preparation:

[0307] Each experimental plot was cleaned to remove weeds, stones and other impurities and to ensure that the soil was loose and free of large lumps of matter.

[0308] Analyze the soil in the experimental fields to ensure that the initial soil pH, moisture, nutrient content and other conditions are similar.

[0309] Fertilizer application:

[0310] Each test field was fertilized with 60 g of fertilizer per square meter. The fertilizer of Example 4 was added with an enzyme-controlled synergist, while the fertilizer of Comparative Example 4 did not contain any synergist. After fertilization, a small rake was used to evenly rake the fertilizer into the soil to ensure that the fertilizer and the soil were fully mixed.

[0311] Data collection:

[0312] Samples were taken from the test field every 7 days to measure the concentrations of phosphorus and nitrogen in the soil. The experiment lasted for 30 days, and the release of fertilizers after each sampling was recorded, and the effectiveness of the fertilizers was calculated through nutrient analysis.

[0313] Experimental period:

[0314] The experimental period for each test field is 30 days, with continuous sampling and data recording.

[0315] Table 4: Fertilizer release rate and nutrient conversion data

[0316]

[0317]

[0318] Experimental Summary

[0319] From the experimental results, the release rate of phosphorus and nitrogen in the fertilizer in Example 4 is significantly higher after application, especially in an environment of 30°C, the release of the fertilizer is significantly accelerated. This shows that the enzyme-controlled synergist can effectively promote the conversion of nutrients such as phosphorus and nitrogen in the fertilizer and improve the nutrient release efficiency of the fertilizer. In contrast, the release rate of the fertilizer in Comparative Example 4 is relatively stable at various temperatures, and the variation range is small.

[0320] From a mechanistic perspective, enzyme-controlled enhancers such as phosphatases and nitrogenases can accelerate the conversion process of nutrients in fertilizers. By increasing the activity of these enzymes, nutrients in fertilizers that are difficult for crops to absorb directly (such as phosphorus and nitrogen) can be converted more quickly into a form that crops can use. This not only improves the efficiency of fertilizer use, but also reduces nutrient loss and waste.

[0321] In general, this experiment verified the important role of enzyme-controlled enhancers in fertilizers, especially in improving fertilizer utilization efficiency and accelerating nutrient conversion. Enzyme-controlled enhancers provide new technical support for precision fertilization, enabling fertilizers to effectively provide required nutrients under different environmental conditions, thereby reducing nutrient loss and increasing crop yields.

[0322] Experiment 5: Comparison between conventional fertilizer and fertilizer of the present invention

[0323] Purpose:

[0324] This experiment aims to verify the advantages of the fertilizer of the present invention in promoting crop growth and improving nutrient absorption efficiency by comparing the effects of using traditional fertilizers with the fertilizer of the present invention. The experiment pays special attention to the nutrient release of the fertilizer under different environments and evaluates the sustainability and environmental adaptability of the new fertilizer.

[0325] sample:

[0326] Embodiment 5: Fertilizer of the present invention

[0327] Conventional fertilizers: Regular market fertilizers without temperature-responsive polymers or other regulators

[0328] Experimental field setting:

[0329] Number and selection of experimental fields:

[0330] Select 4 experimental plots, each with an area of ​​12 square meters (3mx4m), and ensure that the experimental conditions such as soil type, humidity, pH value, etc. are consistent. The experimental plots should be unpolluted and well-drained land.

[0331] Experimental field distribution:

[0332] The experimental fields were divided into two groups:

[0333] Group 1 (Example 5): Fertilizer of Example 5 was applied

[0334] Group 2 (conventional fertilizers): application of conventional fertilizers

[0335] Different temperature conditions were set in the two experimental plots of each group:

[0336] Experimental field 1 (20°C, Example 5): Fertilizer of Example 5 was applied and the temperature was controlled at 20°C.

[0337] Experimental field 2 (20℃, traditional fertilizer): traditional fertilizer was applied and the temperature was controlled at 20℃

[0338] Experimental field 3 (30°C, Example 5): Fertilizer of Example 5 was applied and the temperature was controlled at 30°C.

[0339] Experimental field 4 (30℃, traditional fertilizer): traditional fertilizer was applied and the temperature was controlled at 30℃

[0340] Temperature Control:

[0341] A greenhouse was used to keep the temperature of the experimental field stable. A heating system was used in the 30℃ group, while the temperature of the 20℃ group was regulated by a sunshade net to ensure a stable environment in different groups.

[0342] Steps:

[0343] Soil preparation:

[0344] Pre-treat the test plots to remove weeds, rocks and other debris, and ensure that the soil is loose and maintains appropriate moisture. Perform soil analysis to ensure that the initial conditions of each test plot are consistent and the pH value is controlled between 6.5 and 7.0.

[0345] Fertilizer application:

[0346] Each test field was fertilized with 60 g of fertilizer per square meter. The fertilizer of Example 5 was the fertilizer of the present invention, containing special ingredients such as temperature-responsive polymers; the traditional fertilizer was a conventional commercial fertilizer. After fertilization, a small rake was used to evenly rake the fertilizer into the soil.

[0347] Data collection:

[0348] Soil samples were taken every seven days to measure the concentrations of phosphorus and nitrogen in the soil and to record the growth of the crops. The experiment lasted for 30 days.

[0349] Experimental period:

[0350] The experimental period for each test field is 30 days, during which nutrient changes in the soil are measured regularly and crop growth data are recorded.

[0351] Table 5: Fertilizer release rate and crop growth data

[0352]

[0353]

[0354] Experimental Summary

[0355] The fertilizer in Example 5 showed a faster nutrient release rate than traditional fertilizers, especially at 30°C, the phosphorus and nitrogen release of the fertilizer increased significantly. This shows that the temperature-responsive polymer can adjust the release rate of the fertilizer according to temperature changes, making it more suitable for crop needs. In contrast, the release rate of traditional fertilizers at different temperatures changes less and the release amount is relatively low.

[0356] From a mechanistic analysis, the mechanism of action of temperature-responsive polymers is that they can adjust their solubility in response to temperature changes. When the temperature rises, the solubility of the polymer increases, prompting the nutrients in the fertilizer to be released into the soil faster. In a low temperature environment, the polymer maintains a lower solubility, slowing the release rate of the fertilizer, which helps to reduce nutrient loss.

[0357] The experimental results verify the advantages of the fertilizer of Example 5 in accurately regulating nutrient release. Compared with traditional fertilizers, the fertilizer of the present invention can achieve dynamic regulation at different temperatures, improve the nutrient utilization rate of the fertilizer and reduce excessive loss, thereby effectively improving the absorption efficiency of crops. This provides a new solution for precise fertilization and improving agricultural production efficiency.

[0358] Experiment 6: Comparison of non-colloidal compounds and temperature-responsive polymers

[0359] Purpose:

[0360] The purpose of this experiment is to verify the effect of the combined action of colloidal compounds and temperature-responsive polymers on the fertilizer effect by comparing the fertilizer using colloidal compounds and temperature-responsive polymers with the fertilizer without any polymer, especially the improvement in nutrient release rate and crop growth performance.

[0361] sample:

[0362] Example 6: Use of colloidal compounds and temperature-responsive polymers

[0363] Comparative Example 6: No colloidal compound and temperature-responsive polymer are used

[0364] Experimental field setting:

[0365] Number and selection of experimental fields:

[0366] Four test plots were selected for the experiment, each with an area of ​​15 square meters (3mx5m) to ensure that the soil type and environmental conditions of the test plots were consistent. Each test plot will be used to compare the effects of different fertilizers on nutrient release and crop growth.

[0367] Experimental field distribution:

[0368] The experimental fields were divided into two groups, and different fertilizers were applied to each group:

[0369] Group 1 (Example 6): Fertilizer of Example 6 (containing colloidal compound and temperature-responsive polymer) was applied

[0370] Group 2 (Comparative Example 6): Fertilizer of Comparative Example 6 (without colloidal compound and temperature-responsive polymer) was applied

[0371] Different temperature conditions were set in the two experimental plots of each group:

[0372] Experimental field 1 (20°C, Example 6): Fertilizer of Example 6 was applied and the temperature was controlled at 20°C.

[0373] Experimental field 2 (20°C, Comparative Example 6): Fertilizer of Comparative Example 6 was applied and the temperature was controlled at 20°C.

[0374] Experimental field 3 (30°C, Example 6): Fertilizer of Example 6 was applied and the temperature was controlled at 30°C.

[0375] Experimental field 4 (30°C, comparative example 6): Fertilizer of comparative example 6 was applied and the temperature was controlled at 30°C.

[0376] Temperature Control:

[0377] The temperature control system in the greenhouse was used to control the temperature and ensure the temperature difference between different test plots was stable. The 30℃ group was controlled by a heating system, and the 20℃ group was cooled by a sunshade net.

[0378] Steps:

[0379] Soil preparation:

[0380] Clean the soil in each experimental field, remove weeds and debris, and keep the soil loose and uniform.

[0381] Initial soil analysis was performed to ensure that soil pH, moisture, and nutrient content were similar across all test plots to reduce variable influences.

[0382] Fertilizer application:

[0383] Each test field was fertilized with 60 g of fertilizer per square meter. The fertilizer of Example 6 contained a colloidal compound and a temperature-responsive polymer, while the fertilizer of Comparative Example 6 did not contain any regulator. After fertilization, a small rake was used to evenly rake the fertilizer into the soil to ensure full contact between the fertilizer and the soil.

[0384] Data collection:

[0385] Samples were taken from the experimental fields every 7 days to measure the concentrations of phosphorus and nitrogen in the soil for 30 days. After each sampling, the soil nutrient change data was recorded and sent to the laboratory for analysis.

[0386] Experimental period:

[0387] The experimental period of each test field is 30 days. The nutrient release in the soil is continuously monitored and the growth status of crops is recorded.

[0388] Table 6: Fertilizer effect and nutrient release data

[0389]

[0390]

[0391] Experimental Summary

[0392] The experimental data show that the fertilizer in Example 6 is superior to that in Comparative Example 6 in terms of nutrient release. Under a 30°C environment, the fertilizer release rate of Example 6 is significantly accelerated, especially in the early stage. In contrast, the fertilizer release of Comparative Example 6 is relatively stable and fails to effectively respond to temperature changes. This shows that the combined use of colloidal compounds and temperature-responsive polymers has obvious advantages in regulating the fertilizer release rate.

[0393] From the perspective of mechanism analysis, the combination of colloidal compounds and temperature-responsive polymers can regulate nutrient release by controlling the solubility and diffusivity of fertilizers. Colloidal compounds provide a slower and more stable release environment, while temperature-responsive polymers regulate the dissolution rate of fertilizers by responding to changes in ambient temperature. In low-temperature environments, the release rate of this fertilizer is slower, which helps to prolong the fertilizer effect; in high-temperature environments, the fertilizer release is accelerated to meet the rapid nutrient needs of crops.

[0394] In summary, the experiment verified the significant advantages of the fertilizer of Example 6 in controlling nutrient release and improving nutrient utilization efficiency. Compared with traditional fertilizers and fertilizers without any polymers, the fertilizer of the present invention can dynamically adjust the release of fertilizer according to temperature, improve the absorption effect of crops, and reduce fertilizer waste. The fertilizer provides new technical support for precision fertilization and efficient agriculture.

[0395] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An enzyme-controlled synergist and an enzyme-controlled fertilizer composition, characterized in that: The following mass fractions are included: Enzyme control enhancers, including: Phosphatase, 4-7 copies; Nitrogenase, 3-5 parts; Protease, 2-4 parts; Nitrate reductase, 1-3 parts; Colloidal protective agent, 3 to 5 parts; Organic acid, 2-5 parts; Enzyme-controlled fertilizers, including: Polyethyleneimine modified nano-silicon, 10-15 parts; Temperature responsive polymer, 5 to 10 parts; Nano carbon material, 5 to 10 parts; colloidal compound, 2 to 5 parts; Wherein, the ratio of the enzyme-controlled synergist to the enzyme-controlled fertilizer is 1:5 to 1:

10.

2. The enzyme-controlled synergist and enzyme-controlled fertilizer composition according to claim 1, characterized in that: The phosphatase is obtained by fermentation of a microorganism, and the microorganism comprises one of Bacillus subtilis, Bacillus licheniformis or Penicillium.

3. The enzyme-controlled synergist and enzyme-controlled fertilizer composition according to claim 1, characterized in that: The nitrogenase is a synthetic enzyme, including one or more of nitrate reductase or amino acid transaminase.

4. The enzyme-controlled synergist and enzyme-controlled fertilizer composition according to claim 1, characterized in that: The polyethyleneimine modified nano-silicon is based on silicon dioxide nano-particles, and the surface is chemically modified by amino groups.

5. The enzyme-controlled synergist and enzyme-controlled fertilizer composition according to claim 1, characterized in that: The temperature-responsive polymer is selected from one of polyacrylic acid and polyvinyl alcohol.

6. The enzyme-controlled synergist and enzyme-controlled fertilizer composition according to claim 1, characterized in that: The nano carbon material is one of carbon nano tubes or graphene.

7. The enzyme-controlled synergist and enzyme-controlled fertilizer composition according to claim 1, characterized in that: The colloid protective agent includes one of polyacrylamide or carboxymethyl cellulose.

8. The enzyme-controlled synergist and enzyme-controlled fertilizer composition according to claim 1, characterized in that: The gelatinous compound includes one or more of polyvinyl alcohol, polyacrylate, gelatin, gum arabic or agar.

9. The enzyme-controlled synergist and enzyme-controlled fertilizer composition according to claim 1, characterized in that: The composition is applied by one or more ways of spraying, broadcasting or burying in the soil.

10. The enzyme-controlled synergist and enzyme-controlled fertilizer composition according to claim 1, characterized in that: The composition is used in agricultural planting.

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