Laundry detergent containing biological enzyme and preparation method thereof

Abstract

The invention relates to the technical field of daily chemical detergents, and discloses a laundry detergent containing biological enzyme enzymes and a preparation method thereof.The laundry detergent containing the biological enzyme enzymes is obtained by adding cellulase, alkaline protease, rose essential oil, carboxymethyl cellulose, polypropylene glycol, an antibacterial agent and deionized water into a stirrer and stirring. The positively charged heads of quaternary ammonium salt groups contained in the laundry detergent can be adsorbed to the surfaces of negatively charged bacteria under the action of electrostatic force, and quaternary ammonium salt entering cells can further disturb the composition and structure of cell membranes, so that the normal physiological functions of the cells are influenced, and the bacteria are dead; and after the epoxy group intermediate reacts with N, N-dimethylethylamine, a quaternary ammonium salt group is generated, so that the substitution degree of the quaternary ammonium salt group is improved, and the antibacterial effect of the quaternary ammonium salt group is better.

Description

Technical Field

[0001] The invention relates to the technical field of daily chemical detergents, in particular to a laundry detergent containing biological enzymes and a preparation method thereof. Background Art

[0002] Laundry detergent is a personal clothing washing product that has appeared and developed rapidly in recent years. Compared with washing powder, it has the advantages of better solubility and low energy consumption. Bio-enzyme laundry detergent is a laundry detergent with biological enzymes added to it, which can effectively remove stains, so it is used in laundry detergent. However, the existing laundry detergent has the problem of poor antibacterial effect, which will cause residual bacteria on clothes and affect the health of the skin. For example, patent CN106800980A discloses a laundry detergent and its preparation method. The invention has the advantages of strong decontamination ability, mild and does not hurt the hands, easy to rinse and no residue, which greatly reduces the damage to the skin, but its antibacterial effect has not been improved. Summary of the invention

[0003] 1. Technical issues to be resolved

[0004] In view of the deficiencies of the prior art, the present invention provides a laundry detergent containing biological enzymes and a preparation method thereof, which has good antibacterial and decontamination effects.

[0005] (II) Technical solution

[0006] To achieve the above object, the present invention provides the following technical scheme: a laundry detergent containing biological enzymes, comprising the following components by weight: 0.02-0.06 parts by weight of cellulase, 0.01-0.04 parts by weight of alkaline protease, 1-1.2 parts by weight of rose essential oil, 0.2-0.41 parts by weight of carboxymethyl cellulose, 0.6-0.7 parts by weight of polypropylene glycol, 0.1-0.2 parts by weight of silver-containing antibacterial agent, and 50-70 parts by weight of deionized water.

[0007] Preferably, the preparation method of the silver-containing antibacterial agent is:

[0008] S1. Add N-methyliminodiacetic acid and 3-chloropropylene to acetonitrile solvent, react at 80-90° C. for 10-15 h, cool after reaction, concentrate under reduced pressure, and wash to obtain olefinated N-methyl quaternary ammonium diacetic acid;

[0009] S2. Dissolve the olefinated N-methyl quaternary ammonium salt diacetic acid in a reaction kettle filled with dichloromethane solvent, add m-chloroperoxybenzoic acid oxidant thereto at 2-4°C, stir and mix for 3-6h, raise the temperature to 20-25°C, react for 8-12h, filter, wash and dry after the reaction is completed to obtain an epoxy intermediate;

[0010] S3. The epoxy intermediate is dissolved in a reaction kettle containing anhydrous ethanol solvent, and then N, N-dimethylethylamine is added thereto, and the reaction is stirred at 20-30° C. for 15-22 hours, and the mixture is distilled under reduced pressure and washed with acetone to obtain a quaternary ammonium salt-modified carboxyl intermediate;

[0011] S4. 160-180 mL of thionyl chloride and 2.1-4.5 g of a quaternary ammonium salt-modified carboxylated intermediate were added to the reactor, the reaction was carried out at a temperature of 70-80 ° C, cooled, concentrated under reduced pressure, the product was washed with petroleum ether, and dried to obtain an acyl chloride intermediate;

[0012] S5. 3.21-3.29 g of 3-aminopyridine and 32-35 mmol of triethylamine were added to acetonitrile solvent, stirred and mixed, and then 2.53-2.65 g of the acyl chloride intermediate was added thereto, stirred at room temperature, washed with deionized water, and dried in vacuo to obtain a quaternary ammonium salt-modified pyridine;

[0013] S6. Add 1-1.2 mmol of quaternary ammonium salt-modified pyridine to anhydrous ethanol, heat to 70-77°C, and after it is completely dissolved, add 2-3 mmol of silver trifluoroacetate, condense and reflux at 55-60°C, react for 20-24 hours, centrifuge and filter, wash, and dry to obtain a silver-containing antibacterial agent.

[0014] In the above reaction process, the tertiary amine in N-methyliminodiacetic acid reacts with the chlorine in 3-chloropropylene to generate a quaternary ammonium salt group, and the carboxyl group and the olefin group are introduced at the same time, and then the olefin group is oxidized to an epoxy group under the action of a meta-chloroperbenzoic acid oxidant, and then a ring-opening reaction is carried out with N,N-dimethylethylamine to generate a quaternary ammonium salt group, thereby obtaining a quaternary ammonium salt modified carboxylation intermediate; the carboxyl group in the quaternary ammonium salt carboxylation intermediate is reacted with thionyl chloride to obtain an acyl chloride intermediate, and then the acyl chloride group continues to react with the amino group in 3-aminopyridine to obtain a quaternary ammonium salt modified pyridine, and then a coordination reaction is carried out with silver trifluoroacetate to introduce silver ions (the degree of substitution of silver ions is increased, making the antibacterial effect better), thereby obtaining a silver-containing antibacterial agent; the pyridine group contained therein also has a good antibacterial effect.

[0015] Preferably, the molar ratio of N-methyliminodiacetic acid to 3-chloropropylene in S1 is 1 mmol:(1.12-1.14) mmol.

[0016] Preferably, the molar ratio of olefinated N-methyl quaternary ammonium diacetic acid to meta-chloroperbenzoic acid oxidant in S2 is 1 mmol:(2.33-4.27) mmol.

[0017] Preferably, the molar ratio of the epoxy intermediate to N,N-dimethylethylamine in S3 is 1 mmol:(0.08-1.04) mmol.

[0018] Preferably, the reaction time in S4 is 6-7h.

[0019] Preferably, the stirring time in S5 is 20-30 hours.

[0020] Preferably, the preparation method of the laundry detergent containing biological enzymes is: adding cellulase, alkaline protease, rose essential oil, carboxymethyl cellulose, polypropylene glycol, silver-containing antibacterial agent, and deionized water into a stirrer, stirring for 5-8 minutes, and obtaining the laundry detergent containing biological enzymes.

[0021] (III) Beneficial technical effects

[0022] The invention prepares the laundry detergent containing biological enzyme by adding cellulase, alkaline protease, rose essential oil, carboxymethyl cellulose, polypropylene glycol, silver-containing antibacterial agent and deionized water into a stirrer and stirring the mixture.

[0023] The positively charged head of the quaternary ammonium salt group in the silver-containing antibacterial agent can be adsorbed to the negatively charged surface of the bacteria through electrostatic force. The quaternary ammonium salt entering the cell will further disrupt the composition and structure of the cell membrane, thereby affecting the normal physiological function of the cell, thereby causing bacterial death. In addition, after the epoxy intermediate reacts with N,N-dimethylethylamine, a quaternary ammonium salt group is generated, which increases its degree of substitution and makes its antibacterial effect better. The quaternary ammonium salt group and silver ions contained in the silver-containing antibacterial agent themselves have good antibacterial and decontamination effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is the reaction formula of quaternary ammonium salt modification of carboxylation intermediate. DETAILED DESCRIPTION

[0025] The molecular weight of polypropylene glycol is 900.

[0026] Example 1

[0027] S1. Add N-methyliminodiacetic acid and 3-chloropropylene to an acetonitrile solvent, wherein the molar ratio of N-methyliminodiacetic acid to 3-chloropropylene is 1:1.12, react at a temperature of 80° C. for 10 h, cool after completion, concentrate under reduced pressure, and wash to obtain olefinated N-methyl quaternary ammonium diacetic acid;

[0028] S2. Dissolve the olefinic N-methyl quaternary ammonium salt diacetic acid in a reaction kettle filled with dichloromethane solvent, add meta-chloroperbenzoic acid oxidant thereto at 2-4° C., wherein the molar ratio of olefinic N-methyl quaternary ammonium salt diacetic acid to meta-chloroperbenzoic acid oxidant is 1:2.33, stir and mix for 3 hours, heat to 20° C., react for 8 hours, filter, wash, and dry after the reaction is completed to obtain an epoxy intermediate;

[0029] S3. The epoxy intermediate was dissolved in a reaction kettle containing anhydrous ethanol solvent, and N,N-dimethylethylamine was added thereto, wherein the molar ratio of the epoxy intermediate to N,N-dimethylethylamine was 1:0.08, and the reaction was stirred at 20°C for 15h, and the mixture was distilled under reduced pressure and washed with acetone to obtain a quaternary ammonium salt-modified carboxyl intermediate;

[0030] S4. 160 mL of thionyl chloride and 2.1 g of a quaternary ammonium salt-modified carboxyl intermediate were added to the reactor, and the reaction was carried out at a temperature of 70 ° C for 6 h, cooled, concentrated under reduced pressure, and the product was washed with petroleum ether and dried to obtain an acyl chloride intermediate;

[0031] S5. 3.21 g of 3-aminopyridine and 32 mmol of triethylamine were added to acetonitrile solvent, stirred and mixed, and then 2.53 g of acyl chloride intermediate was added thereto, stirred at room temperature for 20 h, washed with deionized water, and dried in vacuo to obtain quaternary ammonium salt-modified pyridine;

[0032] S6. Add 1 mmol of quaternary ammonium salt-modified pyridine to anhydrous ethanol, heat to 70°C, and after it is completely dissolved, add 2 mmol of silver trifluoroacetate, condense and reflux at 55°C, react for 20 hours, centrifuge and filter, wash, and dry to obtain a silver-containing antibacterial agent;

[0033] S7. Add 0.02 parts by weight of cellulase, 0.01 parts by weight of alkaline protease, 1 part by weight of rose essential oil, 0.2 parts by weight of carboxymethyl cellulose, 0.6 parts by weight of polypropylene glycol, 0.1 parts by weight of silver-containing antibacterial agent, and 50 parts by weight of deionized water into a stirrer and stir for 5 minutes to obtain a laundry detergent containing biological enzymes.

[0034] Example 2

[0035] S1. Add N-methyliminodiacetic acid and 3-chloropropylene to an acetonitrile solvent, wherein the molar ratio of N-methyliminodiacetic acid to 3-chloropropylene is 1:1.14, react at a temperature of 90° C. for 15 h, cool after completion, concentrate under reduced pressure, and wash to obtain olefinated N-methyl quaternary ammonium diacetic acid;

[0036] S2. Dissolve the olefinic N-methyl quaternary ammonium salt diacetic acid in a reaction kettle filled with dichloromethane solvent, add meta-chloroperbenzoic acid oxidant thereto at 2-4° C., wherein the molar ratio of olefinic N-methyl quaternary ammonium salt diacetic acid to meta-chloroperbenzoic acid oxidant is 1:4.27, stir and mix for 6 hours, heat to 25° C., react for 12 hours, filter, wash, and dry after the reaction is completed to obtain an epoxy intermediate;

[0037] S3. The epoxy intermediate was dissolved in a reaction kettle containing anhydrous ethanol solvent, and N,N-dimethylethylamine was added thereto, wherein the molar ratio of the epoxy intermediate to N,N-dimethylethylamine was 1:1.04, and the reaction was stirred at 30°C for 22h, and the mixture was distilled under reduced pressure and washed with acetone to obtain a quaternary ammonium salt-modified carboxyl intermediate;

[0038] S4. 180 mL of thionyl chloride and 4.5 g of a quaternary ammonium salt-modified carboxyl intermediate were added to the reactor, reacted at 80 ° C for 7 h, cooled, concentrated under reduced pressure, and the product was washed with petroleum ether and dried to obtain an acyl chloride intermediate;

[0039] S5. 3.29 g of 3-aminopyridine and 35 mmol of triethylamine were added to acetonitrile solvent, stirred and mixed, and then 2.65 g of the acyl chloride intermediate was added thereto, stirred at room temperature for 30 h, washed with deionized water, and dried in vacuo to obtain a quaternary ammonium salt-modified pyridine;

[0040] S6. Add 1.2 mmol of quaternary ammonium salt-modified pyridine to anhydrous ethanol, heat to 77°C, and after it is completely dissolved, add 3 mmol of silver trifluoroacetate, condense and reflux at 60°C, react for 24 hours, centrifuge and filter, wash, and dry to obtain a silver-containing antibacterial agent;

[0041] S7. Add 0.06 parts by weight of cellulase, 0.04 parts by weight of alkaline protease, 1.2 parts by weight of rose essential oil, 0.41 parts by weight of carboxymethyl cellulose, 0.7 parts by weight of polypropylene glycol, 0.2 parts by weight of silver-containing antibacterial agent, and 70 parts by weight of deionized water into a stirrer and stir for 8 minutes to obtain a laundry detergent containing biological enzymes.

[0042] Example 3

[0043] S1. Add N-methyliminodiacetic acid and 3-chloropropylene to an acetonitrile solvent, wherein the molar ratio of N-methyliminodiacetic acid to 3-chloropropylene is 1:1.13, react at a temperature of 85° C. for 12 h, cool after completion, concentrate under reduced pressure, and wash to obtain olefinated N-methyl quaternary ammonium diacetic acid;

[0044] S2. Dissolve the olefinic N-methyl quaternary ammonium salt diacetic acid in a reaction kettle filled with dichloromethane solvent, add meta-chloroperbenzoic acid oxidant thereto at 3°C, wherein the molar ratio of olefinic N-methyl quaternary ammonium salt diacetic acid to meta-chloroperbenzoic acid oxidant is 1:3.46mmol, stir and mix for 5h, heat to 23°C, react for 10h, filter, wash, and dry after the reaction is completed to obtain an epoxy intermediate;

[0045] S3. The epoxy intermediate was dissolved in a reaction kettle containing anhydrous ethanol solvent, and N,N-dimethylethylamine was added thereto, wherein the molar ratio of the epoxy intermediate to N,N-dimethylethylamine was 1:0.09, and the reaction was stirred at 25°C for 18h, and the mixture was distilled under reduced pressure and washed with acetone to obtain a quaternary ammonium salt-modified carboxyl intermediate;

[0046] S4. 170 mL of thionyl chloride and 2.3 g of a quaternary ammonium salt-modified carboxyl intermediate were added to the reactor, and the reaction was carried out at a temperature of 75 ° C for 6 h, cooled, concentrated under reduced pressure, and the product was washed with petroleum ether and dried to obtain an acyl chloride intermediate;

[0047] S5. 3.26 g of 3-aminopyridine and 34 mmol of triethylamine were added to acetonitrile solvent, stirred and mixed, and then 2.61 g of the acyl chloride intermediate was added thereto, stirred at room temperature for 25 h, washed with deionized water, and dried in vacuo to obtain a quaternary ammonium salt-modified pyridine;

[0048] S6. 1.1 mmol of quaternary ammonium salt-modified pyridine was added to anhydrous ethanol, heated to 75°C, and after it was completely dissolved, 2 mmol of silver trifluoroacetate was added thereto, condensed and refluxed at 57°C, reacted for 22 hours, and after the reaction, centrifuged and filtered, washed, and dried to obtain a silver-containing antibacterial agent;

[0049] S7. Add 0.04 parts by weight of cellulase, 0.03 parts by weight of alkaline protease, 1.1 parts by weight of rose essential oil, 0.34 parts by weight of carboxymethyl cellulose, 0.65 parts by weight of polypropylene glycol, 0.15 parts by weight of silver-containing antibacterial agent, and 60 parts by weight of deionized water into a stirrer and stir for 6 minutes to obtain a laundry detergent containing biological enzymes.

[0050] Example 4

[0051] S1. Add N-methyliminodiacetic acid and 3-chloropropylene to an acetonitrile solvent, wherein the molar ratio of N-methyliminodiacetic acid to 3-chloropropylene is 1:1.12, react at a temperature of 80° C. for 10 h, cool after completion, concentrate under reduced pressure, and wash to obtain olefinated N-methyl quaternary ammonium diacetic acid;

[0052] S2. Dissolve the olefinic N-methyl quaternary ammonium salt diacetic acid in a reaction kettle filled with dichloromethane solvent, add meta-chloroperbenzoic acid oxidant thereto at 2-4° C., wherein the molar ratio of olefinic N-methyl quaternary ammonium salt diacetic acid to meta-chloroperbenzoic acid oxidant is 1:2.33, stir and mix for 3 hours, heat to 20° C., react for 8 hours, filter, wash, and dry after the reaction is completed to obtain an epoxy intermediate;

[0053] S3. The epoxy intermediate was dissolved in a reaction kettle containing anhydrous ethanol solvent, and N,N-dimethylethylamine was added thereto, wherein the molar ratio of the epoxy intermediate to N,N-dimethylethylamine was 1:1.04, and the reaction was stirred at 30°C for 22h, and the mixture was distilled under reduced pressure and washed with acetone to obtain a quaternary ammonium salt-modified carboxyl intermediate;

[0054] S4. 180 mL of thionyl chloride and 4.5 g of a quaternary ammonium salt-modified carboxyl intermediate were added to the reactor, reacted at 80 ° C for 7 h, cooled, concentrated under reduced pressure, and the product was washed with petroleum ether and dried to obtain an acyl chloride intermediate;

[0055] S5. 3.26 g of 3-aminopyridine and 34 mmol of triethylamine were added to acetonitrile solvent, stirred and mixed, and then 2.61 g of the acyl chloride intermediate was added thereto, stirred at room temperature for 25 h, washed with deionized water, and dried in vacuo to obtain a quaternary ammonium salt-modified pyridine;

[0056] S6. 1.1 mmol of quaternary ammonium salt-modified pyridine was added to anhydrous ethanol, heated to 75°C, and after it was completely dissolved, 2 mmol of silver trifluoroacetate was added thereto, condensed and refluxed at 57°C, reacted for 22 hours, and after the reaction, centrifuged and filtered, washed, and dried to obtain a silver-containing antibacterial agent;

[0057] S7. Add 0.04 parts by weight of cellulase, 0.03 parts by weight of alkaline protease, 1.1 parts by weight of rose essential oil, 0.34 parts by weight of carboxymethyl cellulose, 0.65 parts by weight of polypropylene glycol, 0.15 parts by weight of silver-containing antibacterial agent, and 60 parts by weight of deionized water into a stirrer and stir for 6 minutes to obtain a laundry detergent containing biological enzymes.

[0058] Example 5

[0059] S1. Add N-methyliminodiacetic acid and 3-chloropropylene to an acetonitrile solvent, wherein the molar ratio of N-methyliminodiacetic acid to 3-chloropropylene is 1:1.14, react at a temperature of 90° C. for 15 h, cool after completion, concentrate under reduced pressure, and wash to obtain olefinated N-methyl quaternary ammonium diacetic acid;

[0060] S2. Dissolve the olefinic N-methyl quaternary ammonium salt diacetic acid in a reaction kettle filled with dichloromethane solvent, add meta-chloroperbenzoic acid oxidant thereto at 2-4° C., wherein the molar ratio of olefinic N-methyl quaternary ammonium salt diacetic acid to meta-chloroperbenzoic acid oxidant is 1:4.27, stir and mix for 6 hours, heat to 25° C., react for 12 hours, filter, wash, and dry after the reaction is completed to obtain an epoxy intermediate;

[0061] S3. The epoxy intermediate was dissolved in a reaction kettle containing anhydrous ethanol solvent, and N,N-dimethylethylamine was added thereto, wherein the molar ratio of the epoxy intermediate to N,N-dimethylethylamine was 1:0.09, and the reaction was stirred at 25°C for 18h, and the mixture was distilled under reduced pressure and washed with acetone to obtain a quaternary ammonium salt-modified carboxyl intermediate;

[0062] S4. 170 mL of thionyl chloride and 2.3 g of a quaternary ammonium salt-modified carboxyl intermediate were added to the reactor, and the reaction was carried out at a temperature of 75 ° C for 6 h, cooled, concentrated under reduced pressure, and the product was washed with petroleum ether and dried to obtain an acyl chloride intermediate;

[0063] S5. 3.21 g of 3-aminopyridine and 32 mmol of triethylamine were added to acetonitrile solvent, stirred and mixed, and then 2.53 g of acyl chloride intermediate was added thereto, stirred at room temperature for 20 h, washed with deionized water, and dried in vacuo to obtain quaternary ammonium salt-modified pyridine;

[0064] S6. Add 1 mmol of quaternary ammonium salt-modified pyridine to anhydrous ethanol, heat to 70°C, and after it is completely dissolved, add 2 mmol of silver trifluoroacetate, condense and reflux at 55°C, react for 20 hours, centrifuge and filter, wash, and dry to obtain a silver-containing antibacterial agent;

[0065] S7. Add 0.02 parts by weight of cellulase, 0.01 parts by weight of alkaline protease, 1 part by weight of rose essential oil, 0.2 parts by weight of carboxymethyl cellulose, 0.6 parts by weight of polypropylene glycol, 0.1 parts by weight of silver-containing antibacterial agent, and 50 parts by weight of deionized water into a stirrer and stir for 5 minutes to obtain a laundry detergent containing biological enzymes.

[0066] Comparative Example 1

[0067] Compared with Example 5, this comparative example is different in that no silver-containing antibacterial agent is added.

[0068] The laundry detergent containing biological enzymes prepared in each example was taken, and the antibacterial properties of the laundry detergent were measured with reference to QB / T2738-2012 "Evaluation Method for Antibacterial and Antimicrobial Effects of Daily Chemical Products". The specific data are shown in Table 1.

[0069] Table 1: Antimicrobial testing.

[0070] project Escherichia coli Staphylococcus aureus Candida albicans Example 1 99.37 99.67 99.84 Example 2 99.99 99.89 99.99 Example 3 99.96 99.95 99.75 Example 4 99.58 99.99 99.59 Example 5 99.79 99.78 99.98 Comparative Example 1 90.41 90.73 90.64

[0071] It can be seen from Table 1 that the laundry detergent containing biological enzymes prepared in Examples 1-5 of the present invention has better antibacterial effect than that prepared in Comparative Example 1.

[0072] The test method specified in GB / T 13174-2021 "Determination of Detergent Detergent and Circulation Washing Performance for Clothing" was used to test the decontamination ability of the samples of the embodiment and the comparative example on three standard dirty cloths: JB-01 carbon black dirty cloth, JB-02 protein dirty cloth, and JB-03 sebum dirty cloth, and the decontamination ability of each sample was calculated. The experimental conditions are: 250ppm hard water, washing concentration of 2g / L, washing time of 20min, temperature of 10±0.5℃, RQHL vertical decontamination machine specified in the national standard, and rotation speed of 120r / min. See Table 2 for specific data.

[0073] Table 2: Decontamination tests.

[0074]

[0075]

[0076] It can be seen from Table 2 that Examples 1-5 of the present invention have better decontamination effects than Comparative Example 1.

[0077] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A laundry detergent containing biological enzymes, characterized in that: The invention comprises the following components by weight: 0.02-0.06 parts by weight of cellulase, 0.01-0.04 parts by weight of alkaline protease, 1-1.2 parts by weight of rose essential oil, 0.2-0.41 parts by weight of carboxymethyl cellulose, 0.6-0.7 parts by weight of polypropylene glycol, 0.1-0.2 parts by weight of silver-containing antibacterial agent and 50-70 parts by weight of deionized water.

2. The laundry detergent containing biological enzyme according to claim 1, characterized in that: The preparation method of the silver-containing antibacterial agent is: S1. Add N-methyliminodiacetic acid and 3-chloropropylene to acetonitrile solvent, react at 80-90° C. for 10-15 h, cool after reaction, concentrate under reduced pressure, and wash to obtain olefinated N-methyl quaternary ammonium diacetic acid; S2. Dissolve the olefinated N-methyl quaternary ammonium salt diacetic acid in a reaction kettle filled with dichloromethane solvent, add m-chloroperoxybenzoic acid oxidant thereto at 2-4°C, stir and mix for 3-6h, raise the temperature to 20-25°C, react for 8-12h, filter, wash and dry after the reaction is completed to obtain an epoxy intermediate; S3. The epoxy intermediate is dissolved in a reaction kettle containing anhydrous ethanol solvent, and then N, N-dimethylethylamine is added thereto, and the reaction is stirred at 20-30° C. for 15-22 hours, and the mixture is distilled under reduced pressure and washed with acetone to obtain a quaternary ammonium salt-modified carboxyl intermediate; S4. 160-180 mL of thionyl chloride and 2.1-4.5 g of a quaternary ammonium salt-modified carboxylated intermediate were added to the reactor, the reaction was carried out at a temperature of 70-80 ° C, cooled, concentrated under reduced pressure, the product was washed with petroleum ether, and dried to obtain an acyl chloride intermediate; S5. 3.21-3.29 g of 3-aminopyridine and 32-35 mmol of triethylamine were added to acetonitrile solvent, stirred and mixed, and then 2.53-2.65 g of the acyl chloride intermediate was added thereto, stirred at room temperature, washed with deionized water, and dried in vacuo to obtain a quaternary ammonium salt-modified pyridine; S6. Add 1-1.2 mmol of quaternary ammonium salt-modified pyridine to anhydrous ethanol, heat to 70-77°C, and after it is completely dissolved, add 2-3 mmol of silver trifluoroacetate, condense and reflux at 55-60°C, react for 20-24 hours, centrifuge and filter, wash, and dry to obtain a silver-containing antibacterial agent.

3. The laundry detergent containing biological enzyme according to claim 2, characterized in that: The molar ratio of N-methyliminodiacetic acid to 3-chloropropylene in S1 is 1 mmol:(1.12-1.14) mmol.

4. The laundry detergent containing biological enzyme according to claim 2, characterized in that: The molar ratio of olefinated N-methyl quaternary ammonium diacetic acid to meta-chloroperbenzoic acid oxidant in S2 is 1 mmol:(2.33-4.27) mmol.

5. The laundry detergent containing biological enzyme according to claim 2, characterized in that: The molar ratio of the epoxy intermediate to N,N-dimethylethylamine in S3 is 1 mmol:(0.08-1.04) mmol.

6. The laundry detergent containing biological enzyme according to claim 2, characterized in that: The reaction time in S4 is 6-7h.

7. The laundry detergent containing biological enzyme according to claim 2, characterized in that: The stirring time in S5 is 20-30h.

8. A method for preparing a laundry detergent containing biological enzymes as claimed in any one of claims 1 to 7, characterized in that: The preparation method of the laundry detergent containing biological enzymes is as follows: adding cellulase, alkaline protease, rose essential oil, carboxymethyl cellulose, polypropylene glycol, a silver-containing antibacterial agent and deionized water into a stirrer, stirring for 5-8 minutes, and obtaining the laundry detergent containing biological enzymes.

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