Coenzyme strain culture fermentation device and culture method

Abstract

The invention relates to the technical field of strain fermentation, and discloses a coenzyme strain culture fermentation device and a culture method.The coenzyme strain culture fermentation device comprises a fermentation tank, the interior of the fermentation tank is divided into an area I and an area II through a partition plate, and when strain fermentation liquor is placed in the area I and the area II, a liquid level A and a liquid level B are formed respectively; the area I and the area II are controlled to be communicated through a communication assembly, the first stirring module generates a stirring effect in the area I, and the second stirring module generates a stirring effect in the area II. According to the invention, circulation of bottom layer fermentation liquor is realized through the liquid returning module, and when the circulated fermentation liquor enters the area II again, under the effect of the second stirring module, the fermentation liquor can be stirred uniformly; the liquid level B is uniformly covered in a rotary spraying posture, so that when foams exist on the liquid level B, the foams can be eliminated through spraying of the fermentation liquor, all areas on the liquid level B can be impacted by the fermentation liquor through further limitation of the rotary spraying posture, and the foam eliminating effect is more uniform.

Description

Technical Field

[0001] The invention relates to the technical field of bacterial strain fermentation, and more specifically, to a coenzyme bacterial strain culture fermentation device and a culture method. Background Art

[0002] Coenzyme Q10 (CoQ10) is a natural antioxidant that is widely used in health products and cosmetics. Its production usually adopts microbial fermentation method, which has important applications in commercial production.

[0003] The fermentation culture of coenzyme Q10 is carried out in a fermentation tank. During the fermentation process, the metabolic activities of microorganisms will produce a large amount of gas. When it is stirred in the fermentation tank, it comes into contact with the liquid and easily forms bubbles. If they are not broken or merged in time, they will gather to form foam. In addition, since the fermentation culture medium of coenzyme Q10 requires a large amount of nutrients (sugars, amino acids, etc.), these nutrients increase the viscosity of the liquid. When the high-viscosity liquid is stirred, the bubbles are not easy to break quickly, but will form larger and more persistent foam.

[0004] Excessive foam production will significantly reduce the dissolution rate of oxygen, resulting in restricted bacterial growth and affecting the synthesis of fermentation products. In addition, it can easily lead to liquid overflow in the fermentation tank, resulting in waste of fermentation liquid, as well as pollution and damage to equipment and a series of other problems. Summary of the invention

[0005] The present invention provides a coenzyme bacteria culture fermentation device and a culture method, which solve the technical problem in the related art that excessive foam is generated and difficult to eliminate during the fermentation culture of coenzyme Q10.

[0006] The present invention provides a coenzyme bacteria culture fermentation device, comprising a fermentation tank, a partition, a connecting component, a first stirring module and a second stirring module. The interior of the fermentation tank is divided into region I and region II by the partition. When the bacteria fermentation liquid is placed in region I and region II, liquid surface A and liquid surface B are formed respectively. Region I and region II are connected by the connecting component. The first stirring module generates a stirring effect in region I, and the second stirring module generates a stirring effect in region II. The fermentation tank is provided with a liquid return module for driving the fermentation liquid to circulate in area II. The liquid return module cooperates with the rotating action end of the second stirring module. When the circulating fermentation liquid re-enters area II, it is evenly covered on the liquid surface B in a rotating spraying posture under the action of the second stirring module.

[0007] As a further solution of the present invention: the first stirring module includes a first motor, a first rotating shaft and a first stirring paddle, the output end of the first motor is fixedly connected to the first rotating shaft, and the first stirring paddle is spirally installed along the first rotating shaft; The second stirring module includes a second motor, a second rotating shaft and a second stirring paddle. The output end of the second motor is fixedly connected to the second rotating shaft. The second stirring paddle is in the shape of a blade and is symmetrically installed relative to the second rotating shaft.

[0008] As a further solution of the present invention: the liquid return module includes a liquid conduction pump, a lower insert tube, an upper insert tube, a liquid spray cylinder and a rotating joint. The two active ends of the liquid conduction pump are respectively connected to the lower insert tube and the upper insert tube. The liquid conduction pump drives the fermentation liquid from area II along the lower insert tube to the upper insert tube. A liquid spray cylinder is fixedly arranged on the top of the second rotating shaft. Spray holes are evenly opened at the bottom of the liquid spray cylinder. The top of the liquid spray cylinder is connected to the upper insert tube through a rotating joint.

[0009] As a further solution of the present invention: the liquid return module also includes a baffle, which is fixed to the top of the liquid spray cylinder in a through-type manner, and the baffle is circular as a whole, and the vertical section of the baffle is in the shape of an arc with the center convex upward.

[0010] As a further solution of the present invention: there are two partitions, which are respectively arranged at the bottom of area I and the top of area II, and a connecting component for controlling the conduction between area I and area II is arranged between the partitions.

[0011] As a further solution of the present invention: the connecting component includes a connector, a liquid guide groove, a support and an electric push rod. The connector is in a circular tube shape, closed at the top and through at the bottom. A liquid guide groove is provided on the top edge of the connector. The support is symmetrically fixed on the connector. The electric push rod is fixedly installed on the partition at the bottom, and the output end of the electric push rod is fixedly connected to the support.

[0012] As a further solution of the present invention: an inlet is provided at the top of the fermentation tank, and an outlet is provided at the bottom, and both the inlet and the outlet are provided with a closed structure that can be controlled to open and close.

[0013] As a further solution of the present invention: a vent is provided on the top of the fermentation tank, and the vent is provided with a closed structure with a controllable opening degree.

[0014] A method for culturing coenzyme strains, using the above-mentioned coenzyme strain culturing and fermentation device, comprises the following steps: S1, initial culture: seed liquid and other raw materials are introduced into the fermentation tank from the inlet, and the first stirring module is started after entering the area I to fully mix the raw materials to form a fermentation liquid, and the opening and closing degree of the vent is controlled so that the coenzyme bacteria in the fermentation liquid can ferment under sufficient oxygen; S2, fermentation liquid transfer: after the coenzyme bacteria in the fermentation liquid are initially mature, the liquid guide groove is extended into area I through the electric push rod. At this time, the connector establishes a channel connecting area I and area II. Under the action of its own gravity, the fermentation liquid enters area II from area I along the channel; S3, late culture: The coenzyme bacteria in the fermentation liquid in area II fully absorb nutrients and generate a large amount of coenzyme Q10 products with the stirring effect provided by the second stirring module and the circulation effect provided by the return liquid module. At this time, new raw materials can be added to area I for initial culture; S4. Export product: open the export port to export the generated coenzyme Q10 mixed solution.

[0015] The beneficial effects of the present invention are: When the present invention is applied to the fermentation and cultivation of coenzyme Q10 bacterial liquid, the stirring action in the fermenter is first divided into two independent operation areas (i.e., area I and area II). After the stirring in the upper area I is completed, the material is discharged to the lower area II for stirring through the connecting component. In terms of the stirring action level, excessive stirring of a certain part in the entire liquid mixing process can be effectively avoided. In terms of reducing foam production, excessive stirring of the coenzyme Q10 bacterial liquid during fermentation will lead to excessive foam generation, affecting the stability of the entire fermentation system. The layered stirring implemented by the present invention can avoid excessively violent gas-liquid collisions in the same fermentation and cultivation stage, thereby avoiding the rapid aggregation of bubbles in the same area. In addition, the zoned stirring method produces a certain fluidity for the fermented liquid, so that the bubbles in the liquid are more evenly distributed, reducing the possibility of bubbles agglomerating into foam.

[0016] The present invention realizes the circulation of the bottom fermentation liquid through the liquid return module. When the circulated fermentation liquid re-enters the area II, it is evenly covered to the liquid surface B in a rotating spraying posture under the action of the second stirring module. In this way, when there is foam on the liquid surface B, the spraying of the fermentation liquid can eliminate it. Moreover, the further limitation of the rotating spraying posture can make all areas on the liquid surface B be impacted by the fermentation liquid, and the foam elimination effect is more uniform.

[0017] The first stirring module and the second stirring module in the present invention adopt different stirring methods. On the one hand, they adapt to the characteristics of the fermentation culture of coenzyme Q10 bacterial liquid, which requires a high degree of mixing in the early stage and high fluidity in the later stage. The first stirring paddle is set in a spiral shape to provide a low shear mixing effect, and the second stirring paddle is installed symmetrically relative to the second rotating axis in a blade shape, so that the liquid surface of the fermentation liquid can obtain sufficient fluctuations and surges during stirring. On the other hand, the low shear mixing method of the first stirring module makes the liquid surface A relatively flatter, avoiding excessive foam generation in the early stage.

[0018] In the present invention, the liquid return module also drives the rotation of the baffle, so that the fermentation liquid from area I will first contact the rotating baffle when entering area II. Under the centrifugal action of the baffle, the fermentation liquid diffuses outward and enters area II by sliding along the inner wall of the fermentation tank, thereby further reducing the amount of foam generated in area II. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the overall external structure of a coenzyme bacteria culture and fermentation device proposed by the present invention; Figure 2 This is a schematic diagram of the overall internal structure of a coenzyme bacteria culture and fermentation device proposed by the present invention; Figure 3 It is a schematic diagram of the coordination structure of the second stirring module and the liquid return module in a coenzyme bacteria culture and fermentation device proposed by the present invention; Figure 4 It is a detailed structural diagram of a liquid return module in a coenzyme bacteria culture and fermentation device proposed by the present invention; Figure 5 It is a schematic diagram of the switching of the connected components in a coenzyme bacteria culture and fermentation device proposed by the present invention.

[0020] In the figure: 1, fermentation tank; 10, inlet; 11, outlet; 12, vent; 2, partition; 3, connecting component; 301, connector; 302, liquid guide groove; 303, support; 304, electric push rod; 4, first stirring module; 401, first motor; 402, first rotating shaft; 403, first stirring paddle; 5, second stirring module; 501, second motor; 502, second rotating shaft; 503, second stirring paddle; 6, liquid return module; 601, liquid guide pump; 602, lower insert pipe; 603, upper insert pipe; 604, liquid spray cylinder; 6040, spray hole; 605, baffle; 606, rotary joint. DETAILED DESCRIPTION

[0021] The subject matter described herein will now be discussed with reference to example implementations. It should be understood that the discussion of these implementations is only to enable those skilled in the art to better understand and implement the subject matter described herein, and the functions and arrangements of the elements discussed may be changed without departing from the scope of protection of the contents of this specification. Various examples may omit, replace, or add various processes or components as needed. In addition, the features described in some examples may also be combined in other examples.

[0022] At least one embodiment of the present invention discloses a coenzyme bacteria culture fermentation device, such as Figure 1 - Figure 5As shown, it includes a fermentation tank 1, a partition 2, a connecting component 3, a first stirring module 4 and a second stirring module 5. The interior of the fermentation tank 1 is divided into area I and area II by the partition 2. When the bacterial fermentation liquid is placed in area I and area II, liquid levels A and B are formed respectively. The area I and area II are connected by the connecting component 3. The first stirring module 4 generates a stirring effect in area I, and the second stirring module 5 generates a stirring effect in area II. The fermentation tank 1 is provided with a liquid return module 6 for driving the fermentation liquid to circulate in area II. The liquid return module 6 cooperates with the rotating action end of the second stirring module 5. When the circulated fermentation liquid re-enters area II, it is evenly covered on the liquid surface B in a rotating spraying posture under the action of the second stirring module 5.

[0023] When the present invention is applied to the fermentation and cultivation of coenzyme Q10 bacterial liquid, the stirring action in the fermentation tank 1 is first divided into two independent operation areas (i.e., area I and area II). After the stirring in the upper area I is completed, the material is discharged to the lower area II for stirring through the connecting component 3. In terms of the stirring action level, excessive stirring of a certain part in the entire liquid mixing process can be effectively avoided. In terms of reducing foam production, excessive stirring of the coenzyme Q10 bacterial liquid during fermentation will lead to excessive foam generation, affecting the stability of the entire fermentation system. The layered stirring implemented by the present invention can avoid excessive gas-liquid collisions in the same fermentation and cultivation stage, thereby avoiding the rapid aggregation of bubbles in the same area. In addition, the zoned stirring method produces a certain fluidity for the fermentation liquid, so that the bubbles in the liquid are more evenly distributed, reducing the possibility of bubbles agglomerating into foam.

[0024] Furthermore, in order to further eliminate the adverse effects of foam during fermentation culture, the present invention realizes the circulation of the bottom fermentation liquid through the liquid return module 6. When the circulated fermentation liquid re-enters area II, it is evenly covered to the liquid surface B in a rotating spraying posture under the action of the second stirring module 5. In this way, when there is foam on the liquid surface B, the spraying of the fermentation liquid can eliminate it, and the further limitation of the rotating spraying posture can make all areas on the liquid surface B be impacted by the fermentation liquid, and the foam elimination effect is more uniform.

[0025] The first stirring module 4 includes a first motor 401, a first rotating shaft 402 and a first stirring paddle 403. The output end of the first motor 401 is fixedly connected to the first rotating shaft 402. The first stirring paddle 403 is installed along the first rotating shaft 402 in a spiral shape. The second stirring module 5 includes a second motor 501 , a second rotating shaft 502 and a second stirring paddle 503 . The output end of the second motor 501 is fixedly connected to the second rotating shaft 502 . The second stirring paddle 503 is blade-shaped and symmetrically installed relative to the second rotating shaft 502 .

[0026] The first stirring module 4 and the second stirring module 5 adopt different stirring methods. On the one hand, in order to adapt to the characteristics of the fermentation culture of the coenzyme Q10 bacterial liquid, a high degree of mixing is required in the early stage and high fluidity is required in the later stage, the first stirring paddle 403 is set in a spiral shape to provide a low shear mixing effect, and the second stirring paddle 503 is installed symmetrically relative to the second rotating shaft 502 in a blade shape, so that the liquid surface of the fermentation liquid can obtain sufficient fluctuations during stirring. On the other hand, the low shear mixing method of the first stirring module 4 makes the liquid surface A relatively flatter, avoiding excessive foam generation in the early stage.

[0027] The liquid return module 6 includes a liquid conduction pump 601, a lower insert tube 602, an upper insert tube 603, a liquid spraying cylinder 604 and a rotary joint 606. The two active ends of the liquid conduction pump 601 are respectively connected to the lower insert tube 602 and the upper insert tube 603. The liquid conduction pump 601 drives the fermentation liquid from the area II along the lower insert tube 602 to the upper insert tube 603. The liquid spraying cylinder 604 is fixedly arranged on the top of the second rotating shaft 502. The bottom of the liquid spraying cylinder 604 is evenly provided with spray holes 6040. The top of the liquid spraying cylinder 604 is connected to the upper insert tube 603 through a rotary joint 606.

[0028] The liquid return module 6 further includes a baffle 605 , which is fixed to the top of the liquid spraying cylinder 604 in a through manner. The baffle 605 is circular in shape as a whole, and a vertical section of the baffle 605 is in the shape of an arc with the center convex upward.

[0029] The liquid return module 6 also drives the rotation of the baffle 605, so that the fermentation liquid from area I will first contact the rotating baffle 605 when entering area II. Under the centrifugal action of the baffle 605, the fermentation liquid diffuses outward and slides along the inner wall of the fermentation tank 1 into area II, thereby further reducing the amount of foam generated in area II.

[0030] There are two partitions 2, which are respectively arranged at the bottom of area I and the top of area II. A connecting component 3 for controlling the conduction between area I and area II is arranged between the partitions 2.

[0031] The connecting component 3 includes a connector 301, a liquid conducting groove 302, a support 303 and an electric push rod 304. The connector 301 is in the shape of a circular tube, with a closed top and a through bottom. A liquid conducting groove 302 is opened on the top edge of the connector 301. The support 303 is symmetrically fixed on the connector 301. The electric push rod 304 is fixedly installed on the partition 2 located at the bottom, and the output end of the electric push rod 304 is fixedly connected to the support 303.

[0032] Under the pulling action of the electric push rod 304, the connector 301 can slide between the two partitions 2. Figure 5When the connector 301 is in the position shown on the left side, the liquid guide groove 302 is located between the two partitions 2. At this time, the area I and the area II are completely separated. Figure 5 When the fermentation liquid is in the position shown on the right side, the liquid guide groove 302 extends into the area I. At this time, the connector 301 establishes a channel connecting the area I and the area II. Under the action of its own gravity, the fermentation liquid enters the area II from the area I along the channel.

[0033] The fermentation tank 1 is provided with an inlet 10 at the top and an outlet 11 at the bottom. Both the inlet 10 and the outlet 11 are provided with a closed structure that can be controlled to open and close.

[0034] The fermentation liquid and other raw materials required for the fermentation culture of the coenzyme bacteria enter the fermentation tank 1 from the inlet 10 , and the products generated after the fermentation are completed are discharged from the outlet 11 .

[0035] It should be noted that, during the fermentation process, providing a controllable opening and closing closed structure at the inlet 10 and the outlet 11 of the fermentation tank 1 belongs to the prior art and will not be described in detail here.

[0036] A vent 12 is provided on the top of the fermentation tank 1, and the vent 12 is provided with a closed structure with a controllable opening degree.

[0037] By controlling the opening and closing degree of the vent 12, the balance between the air pressure in the fermenter 1 and the outside air pressure is controlled. Similarly, the vent 12 with a closed structure with controllable opening and closing belongs to the scope of the prior art, and its implementation principle will not be described in detail here.

[0038] A method for culturing coenzyme strains, using the above-mentioned coenzyme strain culturing and fermentation device, comprises the following steps: S1, initial culture: raw materials such as seed liquid are introduced into the fermentation tank 1 from the inlet 10, and after entering the area I, the first stirring module 4 is started to fully mix the raw materials to form a fermentation liquid, and the opening and closing degree of the vent 12 is controlled so that the coenzyme strains in the fermentation liquid can ferment under sufficient oxygen; S2, fermentation liquid transfer: after the coenzyme bacteria in the fermentation liquid are initially mature, the liquid guide groove 302 is extended into the area I through the electric push rod 304. At this time, the connector 301 establishes a channel connecting the area I and the area II. Under the action of its own gravity, the fermentation liquid enters the area II from the area I along the channel; S3, late culture: the coenzyme bacteria in the fermentation liquid in area II fully absorb nutrients and generate a large amount of coenzyme Q10 products with the stirring effect provided by the second stirring module 5 and the circulation effect provided by the return liquid module 6. At this time, new raw materials can be added to area I for initial culture; S4, exporting the product: opening the outlet 11 to export the generated coenzyme Q10 mixed solution.

[0039] The above describes an embodiment of the present invention, but this embodiment is not limited to the above-mentioned specific implementation mode. The above-mentioned specific implementation mode is merely illustrative and not restrictive. Under the guidance of this embodiment, ordinary technicians in this field can also make more forms of equivalent embodiments, all of which are protected by this embodiment.

Claims

1. A coenzyme bacteria culture and fermentation device, characterized in that: The invention comprises a fermentation tank (1), a partition (2), a connecting component (3), a first stirring module (4) and a second stirring module (5); the interior of the fermentation tank (1) is divided into a region I and a region II by the partition (2); when the bacterial fermentation liquid is placed in the region I and the region II, a liquid surface A and a liquid surface B are formed respectively; the region I and the region II are connected by the connecting component (3); the first stirring module (4) generates a stirring effect in the region I, and the second stirring module (5) generates a stirring effect in the region II; The fermentation tank (1) is provided with a liquid return module (6) for driving the fermentation liquid to circulate in the region II. The liquid return module (6) cooperates with the rotating action end of the second stirring module (5). When the circulated fermentation liquid re-enters the region II, it is evenly covered on the liquid surface B in a rotating spraying posture under the action of the second stirring module (5).

2. A coenzyme bacteria culture and fermentation device according to claim 1, characterized in that: The first stirring module (4) comprises a first motor (401), a first rotating shaft (402) and a first stirring paddle (403); the output end of the first motor (401) is fixedly connected to the first rotating shaft (402); and the first stirring paddle (403) is installed in a spiral shape along the first rotating shaft (402); The second stirring module (5) comprises a second motor (501), a second rotating shaft (502) and a second stirring paddle (503); the output end of the second motor (501) is fixedly connected to the second rotating shaft (502); and the second stirring paddle (503) is in the shape of a blade and is symmetrically installed relative to the second rotating shaft (502).

3. The coenzyme bacteria culture and fermentation device according to claim 2, characterized in that: The liquid return module (6) comprises a liquid conduction pump (601), a lower insert tube (602), an upper insert tube (603), a liquid spraying cylinder (604) and a rotating joint (606). The two active ends of the liquid conduction pump (601) are respectively connected to the lower insert tube (602) and the upper insert tube (603). The liquid conduction pump (601) drives the fermentation liquid to be transported from the region II along the lower insert tube (602) to the upper insert tube (603). The top of the second rotating shaft (502) is fixedly provided with a liquid spraying cylinder (604). The bottom of the liquid spraying cylinder (604) is evenly provided with spray holes (6040). The top of the liquid spraying cylinder (604) is connected to the upper insert tube (603) via a rotating joint (606).

4. A coenzyme bacteria culture and fermentation device according to claim 3, characterized in that: The liquid return module (6) further comprises a baffle (605), wherein the baffle (605) is fixed on the top of the liquid spraying cylinder (604) in a through-type manner, the baffle (605) is circular in shape as a whole, and a vertical section of the baffle (605) is in the shape of an arc with the center convex upward.

5. The coenzyme bacteria culture and fermentation device according to claim 4, characterized in that: There are two partitions (2) in total, which are arranged at the bottom of area I and the top of area II respectively. A connecting component (3) for controlling the conduction between area I and area II is arranged between the partitions (2).

6. The coenzyme bacteria culture and fermentation device according to claim 5, characterized in that: The connecting component (3) comprises a connector (301), a liquid guide groove (302), a support (303) and an electric push rod (304); the connector (301) is in the shape of a round tube, with a closed top and a through bottom; a liquid guide groove (302) is provided on the top edge of the connector (301); the support (303) is symmetrically fixedly arranged on the connector (301); the electric push rod (304) is fixedly mounted on the partition (2) located at the bottom; and the output end of the electric push rod (304) is fixedly connected to the support (303).

7. The coenzyme bacteria culture and fermentation device according to claim 6, characterized in that: The fermentation tank (1) is provided with an inlet (10) at the top and an outlet (11) at the bottom, and both the inlet (10) and the outlet (11) are provided with a closed structure that can be controlled to open and close.

8. The coenzyme bacteria culture and fermentation device according to claim 7, characterized in that: The top of the fermentation tank (1) is provided with a vent (12), and the vent (12) is provided with a closed structure with a controllable opening degree.

9. A method for culturing a coenzyme strain, using a coenzyme strain culturing and fermentation device according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1, initial culture: raw materials such as seed liquid are introduced into the fermentation tank (1) from the inlet (10), and after entering the area I, the first stirring module (4) is started to fully mix the raw materials to form a fermentation liquid, and the opening and closing degree of the vent (12) is controlled so that the coenzyme bacteria in the fermentation liquid can ferment under sufficient oxygen; S2, fermentation liquid transfer: after the coenzyme bacteria in the fermentation liquid are initially mature, the liquid guide groove (302) is extended into area I through the electric push rod (304), and at this time, the connector (301) establishes a channel connecting area I and area II, and the fermentation liquid enters area II from area I along the channel under the action of its own gravity; S3, late culture: The coenzyme bacteria in the fermentation liquid in area II fully absorb nutrients and generate a large amount of coenzyme Q10 products accompanied by the stirring effect provided by the second stirring module (5) and the circulation effect provided by the return liquid module (6). At this time, new raw materials can be added to area I for initial culture; S4. Export product: Open the outlet (11) to export the generated coenzyme Q10 mixed solution.

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