Biological enzyme quantitative feeding device for protein peptide processing

Abstract

The invention relates to the technical field of biochemical engineering, and particularly provides a biological enzyme quantitative feeding device for protein peptide processing. Comprising a fixing frame, and a plurality of charging barrel assemblies used for containing biological enzymes are evenly distributed and fixedly installed on the fixing frame around the circumference of a vertical shaft; a feeding mechanism for pushing the biological enzyme in the charging barrel assembly downwards to complete feeding is assembled on the fixed frame; and a water cooling layer is arranged in the charging barrel assembly. The feeding device provided by the invention can be matched with an existing protein peptide reaction kettle for use, and is used for improving the feeding quality of the biological enzyme, so that the synthesis efficiency and the synthesis quality of the protein peptide are indirectly improved.

Description

Technical Field

[0001] The invention relates to the technical field of biochemical engineering, and specifically proposes a biological enzyme quantitative delivery device for protein peptide processing. Background Art

[0002] Protein peptides are compounds formed by amino acids connected by peptide bonds and are the basic building blocks of proteins. Protein peptides are divided into natural peptides and synthetic peptides. Natural peptides are synthesized by the body itself, while synthetic peptides are artificially synthesized by chemical or biological methods. Different protein peptides have different functions, so they are widely used in various fields such as medical medicine, nutrition, and cosmetics.

[0003] The synthesis of protein peptides can be carried out in a variety of ways, including chemical synthesis and enzyme-catalyzed synthesis; in the process of enzyme-catalyzed protein peptide synthesis, the enzyme as a catalyst can accelerate the reaction without being consumed, while ensuring the high specificity of the reaction, that is, it only acts on specific substrates, which helps to improve the selectivity and purity of protein peptide products. In the process of enzyme-catalyzed reactions, in order to obtain the ideal peptide chain structure, a series of different types of enzymes are usually used, and as the reaction proceeds, multiple enzymes are added in sequence according to a predetermined process sequence.

[0004] In order to strictly control the reaction conditions and environment, protein peptide synthesis is generally carried out in a specific reactor. The top cover of the reactor is generally provided with a feed port for adding raw materials and biological enzymes, and a feed hopper is generally installed at the feed port. During actual processing, biological enzymes are directly added to the feed hopper one by one, and the existing feeding method has the following problems.

[0005] 1) In the existing processing process, a single feed port is basically used for feeding, so the biological enzyme after feeding is unevenly distributed in the reactor. The biological enzyme itself has the function of catalyzing and accelerating synthesis, so it will cause the local reaction inside the reactor to be too fast, affecting the uniformity of the reaction synthesis. Local too fast reaction also increases the possibility of the generation of by-products and intermediates, affecting the purity of the prepared product.

[0006] 2) During the preparation reaction, a lot of heat is generally released. During the continuous feeding process through the feed hopper, the heat will enter the feed hopper through the feed port, thereby directly acting on the biological enzyme material layer. The biological enzyme is active and most of them are sensitive to temperature. The existing feed hopper generally does not have the corresponding temperature regulation function. Therefore, the influence of heat may cause the biological enzyme to be partially inactivated before participating in the catalytic synthesis, thereby reducing the actual participation amount of each batch of biological enzymes, resulting in insufficient actual use of the biological enzymes. Summary of the invention

[0007] In order to solve the above problems, the present invention provides a device for quantitatively delivering biological enzymes for protein peptide processing, which is used to solve the problems mentioned in the above background technology.

[0008] In order to achieve the above-mentioned purpose, the present invention adopts the following technical scheme to achieve: a quantitative dosing device for biological enzymes for protein peptide processing, comprising a fixed frame, on which a plurality of barrel assemblies for placing biological enzymes are evenly distributed and fixedly installed around the circumference of a vertical axis; the fixed frame is equipped with a feeding mechanism for pushing the biological enzymes in the barrel assembly downward to complete the dosing.

[0009] A water cooling layer is provided in the barrel assembly; the feeding mechanism includes a pushing power assembly mounted on a fixed frame for vertical movement, the moving end of the pushing power assembly is equipped with a switching assembly that rotates and switches around the central axis of multiple barrel assemblies, and the rotating end of the switching assembly is fixed with a pushing component.

[0010] The plurality of barrel assemblies cooperate with the pushing component and are equally spaced into two groups. The pushing component is used to synchronously push and deliver the biological enzymes in a single group of barrel assemblies. When the switching component is switched, the pushing component can push and deliver the biological enzymes in another group of barrel assemblies.

[0011] Preferably, the barrel assembly includes a cylindrical barrel fixed on a fixing frame, a water cooling sleeve is mounted on the outer surface of the barrel, a plurality of fins distributed circumferentially around the barrel are arranged in an inner cavity surrounded by the water cooling sleeve and the barrel, and the fins are in contact with the outer wall of the barrel.

[0012] Preferably, the pushing power assembly includes a lifting frame vertically slidably mounted on a fixed frame, a lead screw is vertically rotatably mounted on the fixed frame, a threaded sleeve is fixed on the lifting frame, and the threaded sleeve is threadedly connected to the lead screw; the switching assembly is fixed at the bottom end of the lifting frame.

[0013] Preferably, the switching assembly includes a swivel seat fixed to the bottom end of the lifting frame, a swivel cylinder is vertically rotatably mounted on the swivel seat, the lead screw vertically passes through the center of the swivel cylinder, a cylinder is horizontally fixed on the swivel seat, a rack is fixed to the output end of the cylinder, and a gear ring meshing with the rack is fixed on the swivel cylinder; the pushing component is fixed to the bottom end of the swivel cylinder.

[0014] Preferably, the pushing component includes a multi-wing plate horizontally fixed to the bottom end of the rotating drum, and a plurality of long shafts equal to the number of a single barrel assembly are vertically fixed to the bottom end of the multi-wing plate, and a pushing disk for extending into the barrel is fixed to the bottom end of each long shaft.

[0015] Preferably, threaded rings and limit retaining rings are sequentially distributed on the upper and lower outer walls of the barrel, the water-cooling sleeve is threadedly connected to the threaded rings, and a sealing pressure ring is provided at the bottom end of the water-cooling sleeve, and the sealing pressure ring is pressed tightly against the bottom end of the limit retaining ring.

[0016] Preferably, the fin includes a plug-in piece and an arc piece fixed on the plug-in piece, the plug-in piece and the arc piece form a T-shape, the thread ring and the limit ring are provided with a plurality of slots corresponding to the plurality of fins one by one, the plug-in piece is plugged into the upper and lower opposite slots on the thread ring and the limit ring, and the arc piece is attached to the outer wall of the barrel.

[0017] Preferably, a feed hopper is provided at the top end of the barrel, and a discharge barrel is provided at the bottom end of the barrel; the fixed frame includes a fixed disk and a fixed plate distributed up and down, and a barrel hole is provided on the fixed disk relative to each barrel assembly, and the barrel is embedded in the barrel hole through the feed hopper and fixed on the fixed disk; the discharge barrel is fixed on the fixed plate; a column with a circular tube structure is vertically fixed between the fixed disk and the fixed plate, and the lead screw passes through the column and is rotatably mounted on the fixed plate.

[0018] Preferably, the side wall of the pushing disk is wrapped with a flexible layer, and when the pushing disk extends into the barrel, the side wall of the pushing disk contacts the inner wall of the barrel.

[0019] The above technical scheme has the following advantages or beneficial effects: the present invention provides a device for quantitatively delivering biological enzymes for protein peptide processing, which is provided with a plurality of barrel assemblies that can be divided into two groups at intervals, and the two groups of barrel assemblies can be used for the delivery and preparation of two batches of biological enzymes, and are equipped with a feeding mechanism that can switch and push different batches of biological enzyme materials. In the process of protein peptide catalytic synthesis, different batches of biological enzymes can be switched and delivered batch by batch, and through the synchronous and dispersed delivery of each group of multiple barrel assemblies, the dispersion and uniformity of the delivery distribution of the biological enzymes in the reactor are improved, the local reaction is avoided to be too fast, a relatively uniform catalytic reaction process is maintained, and the generation of by-products and intermediates is reduced; in addition, the barrel assembly has a water-cooling layer structure, whether it is pushing extruded materials or catalytic synthesis heat generation, the barrel assembly can realize rapid heat exchange, thereby maintaining the stability of the temperature in the barrel, so as to maintain the activity of the biological enzyme and ensure the effective participation of the biological enzyme; in summary, the delivery device provided by the present invention can be used in conjunction with the existing protein peptide reactor to improve the delivery quality of the biological enzyme, and then indirectly improve the synthesis efficiency and synthesis quality of the protein peptide. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The present invention and its features, configurations and advantages will become more apparent by reading the detailed description of non-limiting embodiments with reference to the following drawings. The same reference numerals indicate the same parts throughout the drawings, which are not drawn to scale, with emphasis on illustrating the subject matter of the present invention.

[0021] Figure 1 The present invention is a schematic diagram of the three-dimensional structure of a biological enzyme quantitative delivery device for protein peptide processing.

[0022] Figure 2 It is a three-dimensional structural diagram of a biological enzyme quantitative delivery device for protein peptide processing provided by the present invention under another viewing angle.

[0023] Figure 3 The present invention is a front view of a biological enzyme quantitative delivery device for protein peptide processing.

[0024] Figure 4 It is a three-dimensional structural diagram of the fixing frame.

[0025] Figure 5 It is a three-dimensional cross-sectional view of the barrel assembly.

[0026] Figure 6 It is a three-dimensional structural diagram of the barrel.

[0027] Figure 7 This is a structural diagram of the fin.

[0028] Figure 8 It is a three-dimensional diagram of the water cooling sleeve.

[0029] In the figure: 1. fixed frame; 11. fixed plate; 111. barrel hole; 12. fixed plate; 13. column; 14. guide column; 2. barrel assembly; 21. barrel; 211. feed hopper; 212. discharge barrel; 213. threaded ring; 214. limit ring; 2141. gasket groove; 22. fin; 23. water-cooling sleeve; 231. sealing pressure ring; 3. feeding mechanism; 31. pushing power assembly; 311. lifting frame; 312. threaded sleeve; 313. lead screw; 32. switching assembly; 321. rotary seat; 322. rotary barrel; 3221. gear ring; 323. cylinder; 324. rack; 33. pushing component; 331. multi-wing plate; 332. long shaft; 333. pushing plate. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] In order to enable those skilled in the art to better understand the scheme of the present invention, the present invention is further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0032] like Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown, a device for quantitatively delivering biological enzymes for protein peptide processing comprises a fixed frame 1, on which six barrel assemblies 2 for placing biological enzymes are evenly distributed and fixedly installed around the circumference of a vertical axis; the fixed frame 1 comprises a fixed disk 11 and a fixed plate 12 distributed up and down, and a column 13 of a circular tube structure is vertically welded between the fixed disk 11 and the fixed plate 12, the fixed disk 11 is a regular hexagon, the column 13 is located at the center of the fixed disk 11, and the fixed disk 11 is evenly distributed around the center circumference. Six barrel holes 111 are distributed and opened, and six barrel assemblies 2 are assembled in the six barrel holes 111 one by one; the barrel assembly 2 includes a cylindrical barrel 21, a feed hopper 211 is arranged at the top of the barrel 21, and a discharge barrel 212 is arranged at the bottom of the barrel 21; the barrel 21 is embedded in the barrel hole 111 through the feed hopper 211 and is welded to the fixed plate 11; the discharge barrel 212 is welded to the fixed plate 12; the connection between the discharge barrel 212 and the bottom end of the barrel 21 is funnel-shaped. The protein peptide undergoes an enzyme-catalyzed synthesis reaction in the reactor. The delivery device provided by the present invention is specifically used for delivering the enzyme and is docked with the reactor. Since six barrel assemblies 2 are provided in the device provided by the present invention, six feed ports can be provided on the top cover of the reactor. The discharge barrels 212 of the six barrel assemblies 2 are docked at the six feed ports one by one. In order to achieve the isolation of the internal environment of the reactor and the external environment and to facilitate the temporary storage of the enzyme in the barrel 21, an electric valve for controlling the opening and closing is also installed in the discharge barrel 212. The electric valve can use an existing electric butterfly valve or other adaptable valves. It should be added that in the present embodiment, the selected enzymes are all in solid particles or powder.

[0033] Since biological enzymes are active, most of them are sensitive to temperature, and their activity will decrease or be lost at higher temperatures. Figure 5 , Figure 6 , Figure 7 and Figure 8As shown, a water cooling layer is provided in the barrel assembly 2 to maintain the stability of the temperature of the biological enzyme in the barrel 21 . In the barrel assembly 2, a water-cooling sleeve 23 is sleeved on the outer surface of the barrel 21, and a plurality of fins 22 distributed circumferentially around the barrel 21 are arranged in the inner cavity surrounded by the water-cooling sleeve 23 and the barrel 21; a threaded ring 213 and a limit retaining ring 214 are welded and sleeved on the outer wall of the cylindrical section of the barrel 21 in sequence from top to bottom, the threaded ring 213 is fitted on the lower end of the feed hopper 211, and the limit retaining ring 214 is installed adjacent to the funnel section at the bottom of the barrel 21; the water-cooling sleeve 23 is threadedly connected to the threaded ring 213, and a sealing pressure ring 231 is arranged at the bottom end of the water-cooling sleeve 23, and the sealing pressure ring 231 is pressed against the bottom end of the limit retaining ring 214 by rotating and twisting the water-cooling sleeve 23, and an annular gasket groove 2141 is arranged at the bottom end of the limit retaining ring 214, and a rubber gasket can be placed in the gasket groove 2141 to enhance the sealing performance. The fin 22 includes a plug-in piece and an arc piece welded on the plug-in piece. The plug-in piece and the arc piece form a T-shape. The thread ring 213 and the limit ring 214 are provided with multiple slots corresponding to the multiple fins 22 one by one. The plug-in piece is plugged into the upper and lower opposite slots on the thread ring 213 and the limit ring 214. The arc piece has the same curvature as the outer wall of the barrel 21, and the arc piece is attached to the outer wall of the barrel 21.

[0034] The annular space formed between the water-cooling sleeve 23 and the barrel 21 constitutes a water-cooling layer. A water pipe joint is also provided on the water-cooling sleeve 23 for injecting cooling water into the annular space, and rapid heat exchange between the barrel 21 and the cooling water is achieved through the fins 22; the water-cooling layer wrapped around the outer periphery of the barrel 21 is used to maintain the stability of the temperature inside the barrel 21.

[0035] like Figure 1 , Figure 2 and Figure 3 As shown, the fixed frame 1 is equipped with a feeding mechanism 3 for pushing the biological enzyme in the barrel assembly 2 downward to complete the feeding. The feeding mechanism 3 includes a pushing power assembly 31, two guide pillars 14 are vertically welded on the fixed plate 11, and the pushing power assembly 31 includes a lifting frame 311 slidably mounted on the two guide pillars 14, a threaded sleeve 312 is welded on the lifting frame 311, and a lead screw 313 is threadedly connected on the threaded sleeve 312, and the lead screw 313 vertically passes through the center of the tube of the column 13, and is rotatably mounted on the fixed plate 12 through a bearing, and a drive motor (not shown in the figure) can also be fixedly installed between the top ends of the two columns 13 through a motor plate, and the top end of the lead screw 313 can be fixed on the output shaft of the drive motor.

[0036] like Figure 1 , Figure 2 and Figure 3As shown, the bottom end of the lifting frame 311 is equipped with a switching assembly 32, which includes a rotating seat 321 fixed to the bottom end of the lifting frame 311 by bolts, a rotating cylinder 322 is vertically rotatably mounted on the rotating seat 321, a lead screw 313 vertically passes through the center of the rotating cylinder 322, a cylinder 323 is horizontally fixed on the rotating seat 321 through a cylinder fixing plate, a rack 324 is welded to the output end of the cylinder 323, and a gear ring 3221 meshing with the rack 324 is fixed to the rotating cylinder 322. The bottom end of the rotating cylinder 322 is equipped with a pushing component 33; the pushing component 33 includes a multi-wing plate 331 fixed horizontally to the bottom end of the rotating cylinder 322, and the multi-wing plate 331 is divided into three wing plates, and the bottom end of each wing plate is vertically fixed with a long shaft 332 by bolts, and the bottom end of each long shaft 332 is welded with a pushing disk 333 for extending into the barrel 21. The side wall of the pushing disk 333 is wrapped with a flexible layer. When the pushing disk 333 extends into the barrel 21, the side wall of the pushing disk 333 contacts the inner wall of the barrel 21 to prevent the biological enzyme from entering the top of the pushing disk 333 during the pushing process.

[0037] In the present invention, the six barrel assemblies 2 cooperate with the pushing components 33 and are equally divided into two groups, so there are three barrel assemblies 2 in each group. The switching component 32 is used to drive the pushing component 33 to switch the position with the two groups of barrel assemblies 2. When vertically opposite to one of the groups, the three pushing disks 333 are just located above the three barrels 21. The pushing component 33 is used to synchronously push and release the biological enzymes in a single group of barrel assemblies 2. When the switching component 32 is switched, the pushing component 33 can be switched to the top of another group of barrel assemblies 2, so that the biological enzymes in the other group of barrel assemblies 2 can be pushed and released.

[0038] In the process of enzyme-catalyzed synthesis of protein peptides, the corresponding type of enzyme will be selected according to the synthesis process, and the quantitative preparation of each enzyme will be completed before the formal synthesis. During the actual synthesis, each enzyme can be added in sequence according to the predetermined sequence of the process. Compared with the traditional method of directly adding enzymes to the feed port of the reactor, the present invention provides an enzyme adding device matched with the reactor, which can be added in batches in a dispersed manner during the synthesis process. Specifically:

[0039] In the present invention, the device is divided into two groups of barrel assemblies 2, and each group of barrel assemblies 2 is used to simultaneously release different types or batches of biological enzymes. Therefore, during the synthesis process, the biological enzymes released first can be pre-stored in one of the three barrels 21 of the group, and then the biological enzymes of the next batch can be pre-stored in the three barrels 21 of the other group. It should be noted that the biological enzymes released in each batch can be pre-stored in the three barrels 21 as equally as possible.

[0040] During the synthesis preparation process, when the first batch of bio-enzyme is put in, the pushing component 33 is located directly above the three barrels 21 of the group. Subsequently, driven by the pushing power assembly 31, the pushing component 33 descends, and the pushing disk 333 extends into the barrel 21 below. When the pushing disk 333 extends to a position close to the bio-enzyme material level, the electric valves of the three barrels 21 are opened at the same time. Under the continuous pushing of the pushing disk 333 and the gravity of the material itself, the bio-enzyme is put into the reactor through the feed port from the discharge barrel 212, thereby realizing the synchronous and automatic putting of the bio-enzyme in the three barrels 21. Since the bio-enzyme has the function of accelerating The same batch of biological enzymes is dispersedly released in space by three barrels 21, which replaces the existing single feed port to feed the materials, avoiding excessive local reaction of the biological enzymes in the reactor, promoting the spatial uniformity of the reaction inside the reactor, and ensuring the speed of material feeding through the active pushing of the pushing disk 333. In addition, although the pushing disk 333 pushes and squeezes the biological enzyme material during the pushing process, which will generate a certain amount of heat, the barrel assembly 2 with water cooling function can realize rapid heat transfer, thereby ensuring the stability of the internal temperature of the barrel 21 and avoiding the influence on the activity of the biological enzyme.

[0041] After the first batch of biological enzymes are delivered, the pushing component 33 is raised and reset, and then the position is switched through the switching component 32, and at the scheduled delivery time of the second batch of biological enzymes, the active delivery is completed again through the pushing component 33. During the delivery of the second batch of biological enzymes, the biological enzymes delivered in the third batch can be pre-stored and prepared, so that the delivery and preparation of biological enzymes batch by batch can be completed while the biological enzymes are delivered batch by batch, and the delivery of biological enzymes batch by batch can be completed in an orderly manner, so that the synthesis work can be carried out in an orderly manner.

[0042] In the description of the present invention, it is to be understood that the terms “center”, “longitudinal”, “lateral”, “length”, “width”, “thickness”, “up”, “down”, “vertical”, “horizontal”, “top”, “bottom”, “inside”, “outside”, “clockwise”, “counterclockwise”, “axial”, “radial”, “circumferential”, etc., indicating orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0043] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "connect", "install", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood by specific circumstances.

[0044] The above describes the preferred embodiments of the present invention. It should be understood that the present invention is not limited to the above-mentioned specific embodiments, and the devices and structures that are not described in detail should be understood to be implemented in a common manner in the art; any technician familiar with the art can make many possible changes and modifications without departing from the technical solution of the present invention, or modify them into equivalent embodiments with equivalent changes, which does not affect the essential content of the present invention. Therefore, any simple modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of protection of the technical solution of the present invention.

Claims

1. A device for quantitatively delivering biological enzymes for protein peptide processing, characterized in that: The invention comprises a fixed frame (1), on which a plurality of barrel assemblies (2) for placing biological enzymes are evenly distributed and fixedly mounted around a vertical axis; the fixed frame (1) is equipped with a feeding mechanism (3) for pushing the biological enzymes in the barrel assemblies (2) downward to complete the feeding; wherein: The barrel assembly (2) is provided with a water cooling layer; the feeding mechanism (3) comprises a pushing power assembly (31) mounted on a fixed frame (1) for vertical movement, the moving end of the pushing power assembly (31) is equipped with a switching assembly (32) that rotates and switches around the central axis of the distribution of multiple barrel assemblies (2), and the rotating end of the switching assembly (32) is fixed with a pushing component (33); The plurality of barrel assemblies (2) cooperate with the pushing components (33) and are equally spaced and divided into two groups. The pushing components (33) are used to synchronously push and release the biological enzymes in a single group of barrel assemblies (2). When the switching component (32) is switched, the pushing components (33) can push and release the biological enzymes in another group of barrel assemblies (2).

2. A biological enzyme quantitative delivery device for protein peptide processing according to claim 1, characterized in that: The barrel assembly (2) comprises a cylindrical barrel (21) fixed on a fixing frame (1), the barrel (21) being sheathed with a water-cooling sleeve (23), a plurality of fins (22) distributed circumferentially around the barrel (21) being arranged in an inner cavity enclosed by the water-cooling sleeve (23) and the barrel (21), the fins (22) being in contact with an outer wall of the barrel (21).

3. A biological enzyme quantitative delivery device for protein peptide processing according to claim 2, characterized in that: The pushing power assembly (31) comprises a lifting frame (311) vertically slidably mounted on a fixed frame, a lead screw (313) vertically rotatably mounted on the fixed frame, a threaded sleeve (312) fixed on the lifting frame (311), and the threaded sleeve (312) is threadedly connected to the lead screw (313); the switching assembly (32) is fixed to the bottom end of the lifting frame (311).

4. A biological enzyme quantitative delivery device for protein peptide processing according to claim 3, characterized in that: The switching assembly (32) comprises a rotating seat (321) fixed at the bottom end of the lifting frame (311); a rotating cylinder (322) is vertically rotatably mounted on the rotating seat (321); the lead screw (313) vertically passes through the center of the rotating cylinder (322); a cylinder (323) is horizontally fixed on the rotating seat (321); a rack (324) is fixed at the output end of the cylinder (323); a gear ring (3221) meshing with the rack (324) is fixed on the rotating cylinder (322); and the pushing component (33) is fixed at the bottom end of the rotating cylinder (322).

5. A biological enzyme quantitative delivery device for protein peptide processing according to claim 4, characterized in that: The pushing component (33) comprises a multi-wing plate (331) horizontally fixed at the bottom end of the rotary cylinder (322), and a plurality of long shafts (332) equal in number to the number of the single-group barrel assembly (2) are vertically fixed at the bottom end of the multi-wing plate (331), and a pushing disk (333) for extending into the barrel (21) is fixed at the bottom end of each long shaft (332).

6. A biological enzyme quantitative delivery device for protein peptide processing according to claim 2, characterized in that: The outer wall of the barrel (21) is provided with threaded rings (213) and limit retaining rings (214) in sequence on the upper and lower sides; the water-cooling sleeve (23) is threadedly connected to the threaded ring (213); a sealing pressure ring (231) is provided at the bottom end of the water-cooling sleeve (23); and the sealing pressure ring (231) is pressed tightly against the bottom end of the limit retaining ring (214).

7. A biological enzyme quantitative delivery device for protein peptide processing according to claim 6, characterized in that: The fin (22) comprises a plug-in piece and an arc piece fixed on the plug-in piece, the plug-in piece and the arc piece form a T-shape, the thread ring (213) and the limit stop ring (214) are both provided with a plurality of slots corresponding to the plurality of fins (22) one by one, the plug-in piece is plugged into the slots on the thread ring (213) and the limit stop ring (214) which are opposite to each other in the upper and lower directions, and the arc piece is fitted on the outer wall of the barrel (21).

8. The device for quantitatively delivering biological enzymes for protein peptide processing according to claim 3, characterized in that: A feed hopper (211) is arranged at the top end of the barrel (21), and a discharge barrel (212) is arranged at the bottom end of the barrel (21); the fixed frame (1) comprises a fixed disc (11) and a fixed plate (12) which are arranged vertically; a barrel hole (111) is arranged on the fixed disc (11) relative to each barrel assembly (2); the barrel (21) is embedded in the barrel hole (111) through the feed hopper (211) and is fixed on the fixed disc (11); the discharge barrel (212) is fixed on the fixed plate (12); a column (13) with a circular tube structure is vertically fixed between the fixed disc (11) and the fixed plate (12); the lead screw (313) passes through the column (13) and is rotatably mounted on the fixed plate (12).

9. The device for quantitatively delivering biological enzymes for protein peptide processing according to claim 5, characterized in that: The side wall of the pushing disk (333) is wrapped with a flexible layer, and when the pushing disk (333) extends into the barrel (21), the side wall of the pushing disk (333) contacts the inner wall of the barrel (21).

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