Ultrasonic oscillation device

Abstract

The invention discloses an ultrasonic oscillation device, which comprises a heat conduction part, an ultrasonic oscillation part and an ultrasonic oscillation part, and is characterized in that the heat conduction part is provided with accommodating through holes; an ultrasonic amplitude-change pole in the ultrasonic unit is provided with a containing groove capable of bearing the bottom end of the test tube, the containing groove is formed below the containing through hole and communicated with the containing through hole, and the containing groove and the containing through hole form a containing cavity capable of containing the test tube; and the air cooling unit comprises a heat dissipation piece connected with the heat conduction piece and a cooling air channel connected with the heat dissipation piece, heat in the containing cavity can enter the cooling air channel through the heat conduction piece and the heat dissipation piece, and gas in the cooling air channel can carry the heat in the cooling air channel to the outside. When ultrasonic waves are used for scattering exosome precipitates in a solution, the situation that the exosome is damaged due to temperature rise of the solution can be avoided.

Description

Technical Field

[0001] The present application belongs to the field of bioengineering technology, and specifically relates to an ultrasonic oscillation device. Background Art

[0002] Exosomes are a type of small vesicles secreted by cells. They play an important role in intercellular communication, substance transfer, and the occurrence and development of diseases.

[0003] Exosomes are widely present in various biological fluids, such as blood, saliva, urine, cerebrospinal fluid and breast milk, and almost all types of cells can secrete exosomes. These exosomes contain specific protein molecules according to the source cells, thus having different biological functions, such as cell communication and signal transmission, immune regulation, tissue repair and regeneration, drug delivery system, etc., and the molecular markers contained in exosomes may be used for the diagnosis and monitoring of diseases, such as early detection of diseases by detecting specific RNA or protein in exosomes in the blood.

[0004] During the operation, exosomes are prone to precipitate. When conducting experiments, the exosome precipitate needs to be broken up. Usually, a pipette is used for blowing and the strength needs to be paid attention to during blowing to avoid damaging the exosome structure due to violent blowing. The amount of liquid during blowing should not be too much, otherwise it will affect the quality of blowing and make it difficult to break up the exosome precipitate. In this process, the experience and operation methods of the experimenters will directly affect the time to break up the precipitate and the degree of exosome loss, thereby affecting the subsequent operations. Summary of the invention

[0005] In order to reduce the loss of exosomes and improve the efficiency of breaking up exosome precipitates, the present application provides an ultrasonic oscillation device.

[0006] An ultrasonic oscillation device comprises: a heat conducting member provided with a receiving through hole; an ultrasonic unit, an ultrasonic horn in the ultrasonic unit being provided with a receiving groove capable of supporting the bottom end of a test tube, the receiving groove being arranged below the receiving through hole and being communicated with the receiving through hole, the receiving groove and the receiving through hole forming a receiving cavity capable of placing the test tube; an air cooling unit comprising a heat sink connected to the heat conducting member and a cooling air duct connected to the heat sink, the heat in the receiving cavity being able to enter the cooling air duct through the heat conducting member and the heat sink, the gas in the cooling air duct being able to carry the heat in the cooling air duct to the outside.

[0007] The accommodating through hole and the accommodating groove of the ultrasonic transformer in the present application together constitute a accommodating cavity for placing a test tube, the accommodating groove carries the test tube, and the ultrasonic transformer can transmit ultrasonic waves to the test tube containing the exosome solution, thereby using ultrasonic waves to break up the exosome precipitate. In this process, the ultrasonic wave drives the solution to vibrate, causing the solution to heat up, and the exosomes in the solution are easily damaged. The air cooling unit set in the present application can carry the heat in the solution to the outside through the gas, heat sink, and heat conductor flowing in the cooling air duct, thereby preventing excess heat from damaging the exosomes.

[0008] In one embodiment of the present application, the extension direction of the cooling air duct is in the same direction as the depth direction of the accommodating cavity.

[0009] Since the extension direction of the cooling air duct is the same as the depth direction of the accommodating cavity, the projected length of the accommodating cavity in the extension direction of the cooling air duct is larger, the heat sink can have sufficient length to contact the heat conductor, and the contact area with the heat conductor is larger. The heat in the solution can be transferred to the cooling air duct through the heat conductor and dissipated more quickly, and be carried to the outside by the gas in the cooling air duct, so as to achieve a better cooling effect.

[0010] In one embodiment of the present application, the air cooling unit is provided with two groups of cooling air ducts, and the two groups of cooling air ducts are arranged opposite to each other with respect to the longitudinal section of the accommodating cavity.

[0011] In one embodiment of the present application, a plurality of ventilation grooves are provided on the hole wall of the accommodating through hole around the axis of the accommodating through hole, and the ventilation grooves extend upward to the outside in a direction parallel to the axis of the accommodating through hole and are connected with the outside; the cooling air duct is connected with the ventilation grooves through the first air duct.

[0012] Through the ventilation groove connected to the cooling air duct, the gas can flow directly in the ventilation groove to take away the heat in the solution. At the same time, the heat of the solution can also be transferred to the cooling air duct through the heat conductor and the heat sink. The gas can cool the solution through the ventilation groove and the cooling air duct.

[0013] In one embodiment of the present application, an air guide plate is provided in the cooling air duct, and the air guide plate is provided at the connecting point between the cooling air duct and the ventilation groove. Under the guiding action of the air guide plate, the gas blown toward the air guide plate in the cooling air duct enters the ventilation groove through the first air duct, and enters the outside through the ventilation groove.

[0014] Under the action of the air guide plate, the air flowing in the cooling air duct can quickly enter the ventilation groove and carry the heat of the solution into the outside world, ensuring that the temperature of the solution is within an appropriate range and preventing the exosomes from being damaged due to excessive temperature.

[0015] In one embodiment of the present application, the ultrasonic horn is spaced apart from the heat conducting member, and the ultrasonic horn is not in contact with the heat conducting member.

[0016] In one embodiment of the present application, the axis of the accommodating groove, the axis of the accommodating through hole, and the axis of the ultrasonic horn are collinear.

[0017] In one embodiment of the present application, the axis of the ultrasonic horn is arranged vertically, and a receiving groove is provided at the top end of the ultrasonic horn.

[0018] In one embodiment of the present application, the ultrasonic unit further includes an ultrasonic transducer connected to the ultrasonic amplitude transformer; the ultrasonic oscillation device further includes a base, and the ultrasonic transducer and the ultrasonic amplitude transformer are connected to the base via a shock absorber.

[0019] In one embodiment of the present application, the cooling air duct is connected to a fan.

[0020] The present application has at least the following beneficial effects:

[0021] 1. The accommodating through hole and the accommodating groove of the ultrasonic horn in the present application together constitute a accommodating cavity for placing a test tube. The accommodating groove carries the test tube, and the ultrasonic horn can transmit ultrasonic waves to the test tube containing the exosome solution, thereby using ultrasonic waves to break up the exosome precipitate. In this process, the ultrasonic wave drives the solution to vibrate, causing the solution to heat up, and the exosomes in the solution are easily damaged. The air cooling unit set in the present application can carry the heat in the solution to the outside through the gas, heat sink, and heat conductor flowing in the cooling air duct, thereby preventing excess heat from damaging the exosomes.

[0022] 2. Through the ventilation groove connected to the cooling air duct, the gas can flow directly in the ventilation groove to take away the heat in the solution. At the same time, the heat of the solution can also be transferred to the cooling air duct through the heat conductor and the heat sink. The gas can cool the solution through the ventilation groove and the cooling air duct. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a structural schematic diagram of an exemplary implementation of the present application;

[0024] Figure 2 is a schematic structural diagram of a schematic implementation of the heat dissipation fin in the present application;

[0025] Figure 3 It is a structural schematic diagram of an exemplary embodiment when the housing in the present application is provided with a hole;

[0026] Figure 4 is a schematic structural diagram of an exemplary implementation of the fan in the present application;

[0027] Figure 5 It is a structural schematic diagram of an exemplary implementation mode when the detection tube in the present application is inserted into the accommodating cavity through the housing;

[0028] Figure 6 It is a structural schematic diagram of an exemplary implementation mode when the detection tube in the present application is located in the accommodating cavity;

[0029] Figure 7 is a schematic structural diagram of a schematic implementation of the ventilation groove in the present application;

[0030] Figure 8 is a structural schematic diagram of another exemplary embodiment of the heat conducting element in the present application;

[0031] Fig. 9 It is a structural schematic diagram of an illustrative implementation method when the air guide plate and the heat conducting member in the present application are connected.

[0032] In the figure:

[0033] 101, heat conducting member; 102, receiving through hole; 103, ventilation groove; 104, first air duct;

[0034] 201, ultrasonic horn; 202, receiving groove; 203, ultrasonic transducer; 204, shock absorbing member; 205, base; 206, housing;

[0035] 301, fan; 302, heat sink; 303, cooling air duct; 304, air guide plate;

[0036] 401. Test tube. DETAILED DESCRIPTION

[0037] In order to have a clearer understanding of the technical features, purposes and effects of the present application, the specific implementation methods of the present application are now described with reference to the accompanying drawings. The same reference numerals in the drawings represent components with the same structure or similar structures but the same functions.

[0038] In this document, “exemplary” means “serving as an example, instance or illustration”, and any diagram or implementation described in this document as “exemplary” should not be interpreted as a more preferred or more advantageous technical solution.

[0039] In order to simplify the drawings, only the parts related to the present application are schematically shown in each figure, and they do not represent the actual structure of the product. In addition, in order to simplify the drawings and facilitate understanding, in some figures, only one of the parts with the same structure or function is schematically drawn or marked.

[0040] See also Figures 1 to 9 Understand this application.

[0041] See also Figures 1 to 6 An ultrasonic oscillation device includes: a heat conductor 101, which is provided with a receiving through hole 102; an ultrasonic unit, an ultrasonic horn 201 in the ultrasonic unit is provided with a receiving groove 202 capable of supporting the bottom end of a test tube 401, the receiving groove 202 is arranged below the receiving through hole 102 and is connected to the receiving through hole 102, the receiving groove 202 and the receiving through hole 102 constitute a receiving cavity capable of placing the test tube 401.

[0042] When it is necessary to break up the exosome precipitate in the solution, the solution is placed in the test tube 401, and then the test tube 401 is placed in the containing cavity, the opening of the test tube 401 faces upward, the bottom of the test tube 401 is placed in the containing groove 202 of the ultrasonic horn 201, and the containing through hole 102 prevents the test tube 401 from tilting, and then the ultrasonic transducer 203 in the ultrasonic unit is started. The ultrasonic transducer 203 is a device that converts electromagnetic energy into mechanical energy (acoustic energy), and is usually made of piezoelectric ceramics or other magnetostrictive materials. The piezoelectric effect or magnetostrictive effect of these materials is used to convert electrical signals into mechanical vibrations, thereby generating ultrasonic waves. The ultrasonic horn 201 is connected to the ultrasonic transducer 203, and the ultrasonic horn 201 amplifies the particle displacement or velocity of the mechanical vibration, and concentrates the ultrasonic energy at the containing groove 202, oscillating the solution in the test tube 401, so that the exosome precipitate in the solution is broken up and dispersed into the solution.

[0043] See also Figure 1 In one embodiment of the present application, the ultrasonic unit also includes an ultrasonic transducer 203 connected to the ultrasonic horn 201; the ultrasonic oscillation device also includes a base 205, and the ultrasonic transducer 203 and the ultrasonic horn 201 are connected to the base 205 through a shock absorber 204. The shock absorber 204 can be made of elastic materials such as silicone and rubber. The shock absorber 204 can absorb the energy of the vibration of the ultrasonic transducer 203 and the ultrasonic horn 201, reduce the vibration transmitted to the base 205, avoid causing the overall vibration of the ultrasonic oscillation device, thereby ensuring the normal operation of the ultrasonic oscillation device.

[0044] Since the ultrasonic unit drives the solution to vibrate to break up the exosome precipitation, the temperature of the solution is likely to rise due to the vibration. In order to ensure that the temperature of the solution is within a suitable range to avoid damaging the exosomes, the ultrasonic oscillation device is also provided with an air cooling unit. The air cooling unit includes a heat sink 302 connected to the heat conductor 101 and a cooling air duct 303 connected to the heat sink 302. The heat in the accommodating cavity can enter the cooling air duct 303 through the heat conductor 101 and the heat sink 302, and the gas in the cooling air duct 303 can carry the heat in the cooling air duct 303 to the outside.

[0045] See also Figure 1, the heat sink 302 is a heat sink fin, and there is a gap between the heat sink fins. The housing 206 is provided with holes to form a cooling air duct 303. The cooling air duct 303 is connected to a fan 301. Both ends of the cooling air duct 303 are connected to the outside world. The fan 301 is provided at the heat sink fin. When the fan 301 rotates, it drives the outside air into the cooling air duct 303. The outside air flows in the cooling air duct 303 and finally flows to the outside world. The heat conductor 101 and the heat sink 302 can be made of materials with good thermal conductivity such as copper and aluminum. When the temperature in the solution rises and is higher than the gas temperature in the cooling air duct 303, the temperature in the solution can reach the cooling air duct 303 through the heat conductor 101 and the heat sink 302. The gas flowing in the cooling air duct 303 can carry heat to the outside world, thereby reducing the temperature in the solution and avoiding the situation where the temperature in the solution is too high and damages the exosomes.

[0046] In one embodiment of the present application, the extension direction of the cooling air duct 303 is in the same direction as the depth direction of the accommodating cavity. Since the extension direction of the cooling air duct 303 is in the same direction as the depth direction of the accommodating cavity, the projection length of the accommodating cavity in the extension direction of the cooling air duct 303 is larger. Figure 1 The cooling air duct 303 is vertically arranged, and the depth direction of the accommodating cavity is vertically arranged. The heat sink 302 can have a sufficient length to contact the heat conductor 101, and the distance between the heat sink 302 and the accommodating cavity is small, and the contact area with the heat conductor 101 is larger. The heat in the solution can be transferred to the cooling air duct 303 through the heat conductor 101 more quickly, and carried to the outside by the gas in the cooling air duct 303 to achieve a better cooling effect.

[0047] In one embodiment of the present application, the air cooling unit is provided with two sets of cooling air ducts 303, and the two sets of cooling air ducts 303 are arranged relative to each other with respect to the longitudinal section of the accommodating cavity, see Figure 1 , Figure 2 A group of heat dissipation fins are provided on each side of the accommodating cavity, and the outer shell 206 is provided with two groups of holes connected to the gaps between the heat dissipation fins, thereby forming two groups of cooling air ducts 303. The two groups of cooling air ducts 303 are respectively connected to fans 301, so that under the action of the fans 301, external air can enter the two groups of cooling air ducts 303 and carry heat to the outside.

[0048] Those skilled in the art of the present application will understand that there may be many air flow modes in the present application, see Figure 1 ,by Figure 1 The air can flow from top to bottom or from bottom to top. Under the action of the fan 301, the outside air can enter the cooling air duct 303 and bring the heat of the heat sink 302 to the outside.

[0049] Those skilled in the art of the present application can understand that the formation of the cooling air duct 303 in the present application is not limited to being composed of the housing 206 and the heat dissipation fins, but can also be formed by the pores between the heat dissipation fins alone, see Figure 7 Under the action of the fan 301, the outside air flows between the heat dissipation fins to take away the heat of the solution in the test tube 401; of course, the shape of the cooling air duct 303 can also be other shapes, which will not be repeated here.

[0050] See also Figure 7 In one embodiment of the present application, the hole wall of the accommodating through hole 102 is provided with a plurality of ventilation grooves 103 around the axis of the accommodating through hole 102, and the ventilation grooves 103 extend upward to the outside in a direction parallel to the axis of the accommodating through hole 102, and are connected with the outside; the cooling air duct 303 is connected with the ventilation grooves 103 through the first air duct, so that the cooling air duct 303 can exchange gas with the ventilation grooves 103, and the flowing air in the ventilation grooves 103 can directly carry the excess heat in the solution to the outside, thereby ensuring that the temperature of the solution in the test tube 401 is within a suitable range. The first air duct can be formed by connecting the holes provided by the heat conducting member 101 and the heat dissipating member 302, or it can be formed as follows Figure 8 , Fig. 9 As shown, the first air duct 104 is formed by the heat conducting member 101, and the ventilation groove 103 can be connected to the cooling air duct 303 through the first air duct 104, as shown in FIG. Figure 7 As shown, the first air duct 104 is connected to the gap between the heat dissipation fins, and thus connected to the cooling air duct 303 .

[0051] In one embodiment of the present application, an air guide plate 304 is provided in the cooling air duct 303, and the air guide plate 304 is provided at the connecting point between the cooling air duct 303 and the ventilation groove 103. Under the guiding action of the air guide plate 304, the gas blown toward the air guide plate 304 in the cooling air duct 303 enters the ventilation groove 103 through the first air duct 104, and enters the outside through the ventilation groove 103.

[0052] See also Figure 7 The fan 301 is only arranged in the cooling air duct 303. Under the action of the fan 301, the gas in the cooling air duct 303 is blown to the air guide plate 304. The air guide plate 304 is inclined relative to the gas flow direction. Under the guiding action of the air guide plate 304, the gas blown from the air guide plate 304 in the cooling air duct 303 enters the ventilation groove 103 through the first air duct 104, and enters the outside through the ventilation groove 103. The gas passing through the ventilation groove 103 can directly take away the excess heat in the solution. At the same time, the heat conductive element 101 can also transfer the excess heat in the solution to the heat sink 302. The gas in the cooling air duct 303 can bring the heat of the heat sink 302 to the outside, thereby bringing the heat in the solution to the outside faster.

[0053] Technicians in the technical field to which the present application belongs can understand that other methods can also be used to guide the gas in the cooling duct 303 to the first duct, such as the cooling duct 303 includes a cooling main path and a cooling branch path, both the cooling main path and the cooling branch path pass through the heat dissipation component, and the angle between the first duct and the cooling branch path is set, such as the first duct and the cooling branch path are symmetrically arranged with respect to the extension direction of the cooling main path, and are connected with the cooling main path, and the fan 301 is arranged in the cooling main path, and when the gas moves from the cooling main path to the cooling branch path, it can also flow into the first duct 104, which will not be repeated here.

[0054] Furthermore, the ultrasonic oscillation device also includes a controller and a temperature sensor capable of detecting the temperature of the solution. The controller can be a CPU or a PLC. The fan 301 and the temperature sensor are electrically connected to the controller. The temperature sensor transmits a temperature signal to the controller, and the controller controls the start and stop of the fan 301 according to the temperature signal.

[0055] Furthermore, the ultrasonic oscillation device also includes a semiconductor refrigerator, which is electrically connected to the controller. The controller controls the start and stop of the semiconductor refrigerator according to the temperature signal to adjust the temperature of the solution. The semiconductor refrigerator can be directly arranged on the heat conductor 101, and can also be connected to the heat dissipation fins to cool the gas flowing through the containing cavity and cool the solution. Therefore, the present application can utilize air cooling and semiconductor refrigerators to perform more precise temperature control of the solution.

[0056] Those skilled in the art of the present application can understand that a receiving groove air duct can be provided, and the gas in the cooling air duct 303 flows through the receiving groove air duct to cool the receiving groove 202 to protect the exosomes.

[0057] See also Figure 1 In one embodiment of the present application, the ultrasonic horn 201 is spaced apart from the heat conductor 101, and the ultrasonic horn 201 does not contact the heat conductor 101, so as to avoid the ultrasonic horn 201 transmitting vibration to the heat conductor 101, thereby affecting the breakup of exosome precipitation.

[0058] See also Figure 1 In one embodiment of the present application, the axis of the receiving groove 202, the axis of the receiving through hole 102, and the axis of the ultrasonic horn 201 are collinear, so that the ultrasonic horn 201 can directly transmit the vibration of the ultrasonic transducer to the receiving groove 202 to break up the exosome precipitation.

[0059] Furthermore, in one embodiment of the present application, the axis of the ultrasonic horn 201 is arranged vertically, and a receiving groove 202 is provided at the top of the ultrasonic horn 201, so that the ultrasonic horn 201 can better support the test tube 401 containing the exosome precipitate.

[0060] Those skilled in the art to which the present application belongs can understand that the ultrasonic oscillation device is not limited to breaking up exosome precipitation, but can also be used to break up other substances, such as aggregated cells, which will not be elaborated here.

[0061] It should be understood that although this specification is described according to various implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

[0062] The series of detailed descriptions listed above are merely specific descriptions of feasible embodiments of the present application. They are not intended to limit the scope of protection of the present application. Any equivalent implementation scheme or changes that do not deviate from the technical spirit of the present application, such as combination, division or repetition of features, should be included in the scope of protection of the present application.

Claims

1. An ultrasonic oscillation device, characterized in that: include: A heat conducting member provided with a receiving through hole; An ultrasonic unit, wherein the ultrasonic horn in the ultrasonic unit is provided with a receiving groove capable of supporting the bottom end of the test tube, the receiving groove is provided below the receiving through hole and is communicated with the receiving through hole, and the receiving groove and the receiving through hole form a receiving cavity capable of placing the test tube; The air cooling unit includes a heat sink connected to the heat conductive element and a cooling air duct connected to the heat sink. The heat in the accommodating cavity can enter the cooling air duct through the heat conductive element and the heat sink, and the gas in the cooling air duct can carry the heat in the cooling air duct to the outside.

2. An ultrasonic oscillation device according to claim 1, characterized in that: The extending direction of the cooling air duct is in the same direction as the depth direction of the accommodating cavity.

3. An ultrasonic oscillation device according to claim 1, characterized in that: The air cooling unit is provided with two groups of cooling air ducts, and the two groups of cooling air ducts are arranged opposite to each other with respect to the longitudinal section of the accommodating cavity.

4. The ultrasonic oscillation device according to claim 1, characterized in that: The hole wall of the accommodating through hole is provided with a plurality of ventilation grooves around the axis of the accommodating through hole, and the ventilation grooves extend upward to the outside in a direction parallel to the axis of the accommodating through hole and communicate with the outside; The cooling air duct is communicated with the ventilation groove through the first air duct.

5. An ultrasonic oscillation device according to claim 4, characterized in that: An air guide plate is provided in the cooling air duct, and the air guide plate is provided at the connection point between the cooling air duct and the ventilation groove. Under the guiding action of the air guide plate, the gas blown toward the air guide plate in the cooling air duct enters the ventilation groove through the first air duct and enters the outside through the ventilation groove.

6. The ultrasonic oscillation device according to claim 1, characterized in that: The ultrasonic horn is spaced apart from the heat conducting member, and the ultrasonic horn does not contact the heat conducting member.

7. The ultrasonic oscillation device according to claim 1, characterized in that: The axis of the accommodating groove, the axis of the accommodating through hole, and the axis of the ultrasonic horn are collinear.

8. An ultrasonic oscillation device according to claim 7, characterized in that: The axis of the ultrasonic horn is arranged vertically, and the top end of the ultrasonic horn is provided with the accommodating groove.

9. The ultrasonic oscillation device according to claim 1, characterized in that: The ultrasonic unit also includes an ultrasonic transducer connected to the ultrasonic horn; The ultrasonic oscillation device also includes a base, and the ultrasonic transducer and the ultrasonic amplitude transformer are connected to the base via a shock absorbing member.

10. The ultrasonic oscillation device according to claim 1, characterized in that: The cooling air duct is connected with a fan.

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