Cell culture device and culture method

Abstract

The invention relates to the technical field of cell culture, and discloses a cell culture device and a cell culture method.The cell culture device comprises a cell culture box and culture flasks, a central channel is formed in the central position in the cell culture box, an opening is formed in the upper end of the central channel in a penetrating mode, and a sealing cover is in threaded connection with the interior of the opening; a taking and placing opening is formed in the middle of the sealing cover in a penetrating mode, a controller is movably installed in the taking and placing opening, and a first multi-stage air cylinder is fixedly installed at the bottom of the interior of the central channel. Temperature fluctuation or pollution to the main culture environment is avoided, the culture bottle is completely isolated in the pre-adjusting stage, external microorganisms are prevented from polluting the main culture environment, the pollution risk is reduced, and the device has the advantages of being high in practicability and automation degree.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, in particular to a cell culture device and a culture method. Background Art

[0002] Cell culture is crucial in biomedical research, drug development and industrial production. The automation of its operation process and low contamination risk have always been the core goals of technological innovation. Traditional cell culture is highly dependent on manual operations, including the placement and removal of culture bottles, environmental parameter adjustment and process monitoring, which leads to low efficiency and prone to human errors. In addition, frequent manual operations also increase the risk of cross-contamination, affecting the accuracy and repeatability of experimental results. Therefore, how to improve operational accuracy, reduce human intervention, and effectively reduce contamination risks through automation technology has become a key challenge in the development of current cell culture technology. Automated cell culture devices are designed to solve these problems and improve experimental efficiency and data reliability by integrating advanced sensors and control systems.

[0003] In recent years, with the upgrading of biotechnology needs, automated equipment has gradually become a research hotspot, but existing technologies still face the following limitations: Contamination risk: manual opening of lids, transfer of culture bottles and other operations can easily introduce microbial or aerosol contamination, especially in long-term culture or high-sensitivity experiments, the contamination rate increases significantly; Efficiency bottleneck: frequent intervention is required during batch culture, and the operation is time-consuming and difficult to standardize. For example, the placement of culture bottles from different batches relies on manual records, which can easily lead to confusion; Environmental disturbance: manual operation may destroy the stability of temperature and humidity in the incubator and affect the consistency of cell growth.

[0004] In order to reduce human intervention, existing technologies attempt to achieve partial automation through robotic arms, conveyor belts or robots, but their designs still have obvious shortcomings: Partial automation: Most equipment only achieves automation of a single link (such as transfer or temperature control), and the coordination between modules is poor and still requires manual connection. Therefore, it is necessary to design a cell culture device and culture method that is highly practical and highly automated. Summary of the invention

[0005] The object of the present invention is to provide a cell culture device and a culture method to solve the problems raised in the above background technology.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions: a cell culture device, comprising a cell culture box and a culture bottle, wherein a central channel is provided at a central position in the cell culture box, an opening is provided at the upper end of the central channel and a sealing cover is threadedly connected to the opening, a take-in and put-out port is provided at the middle position of the sealing cover and a controller is movably installed in the take-in and put-out port, a multi-stage cylinder 1 is fixedly installed at the bottom of the central channel, a sealing member is provided at the top of the multi-stage cylinder 1 and an RFID reader is provided on the sealing member, a micro-control space is formed between the sealing member and the sealing member, and the RFID reader is used to control the culture bottle For identification, the controller is respectively connected to the multi-stage cylinder 1 and the RFID card reader through wireless communication. The inner wall of the cell culture box is respectively provided with a culture space and a placement space. The culture space and the placement space are relatively arranged on both sides of the central channel. The culture space and the placement space are arranged in a linear array along the cell culture box and in a circular array at the same level. A pick-and-place component is provided in the placement space for taking and placing the culture bottle into the corresponding culture space for culture. A temperature control component is provided in the cell culture box, and the temperature control component controls the temperature of the micro-control space and the cell culture box respectively.

[0007] According to the above technical solution, the sealing member includes a bottom plate, an annular side plate, an L-shaped plate, a multi-stage cylinder 2 and a bottom positioning member 1. The bottom plate is fixedly mounted on the telescopic end of the multi-stage cylinder 1 and the side wall of the bottom plate is movably sleeved with the annular side plate. The multi-stage cylinder 2 is fixedly mounted on the edge of the lower end of the bottom plate and the telescopic end of the multi-stage cylinder 2 is fixedly mounted with the L-shaped plate. The other end of the L-shaped plate is fixedly mounted on the lower end of the annular side plate. A positioning groove is provided in the middle position of the upper end of the bottom plate. The bottom positioning member 1 is located in the positioning groove. The bottom positioning member 1 is used for bottom positioning of the culture bottle. The RFID card reader is located on the bottom positioning member 1. A wireless communication connection is set between the controller and the multi-stage cylinder 2.

[0008] According to the above technical solution, the culture space includes a conveying channel, a culture area and a yield area. The conveying channel is connected to the central channel. The culture area is arranged on both sides of the conveying channel in a mirror-symmetrical manner. A conveying frame is arranged in the conveying channel in a mirror-symmetrical manner. A conveying groove is commonly provided at opposite ends of the conveying frame. The conveying frame extends into the culture area and is provided with a positioning groove. The positioning groove positions the culture bottle for placement and culture. The yield area is provided at the bottom of the conveying channel. A conveying member is provided in the yield area. The conveying member positions and conveys the culture bottle. A bottom positioning member 2 is provided on the conveying member. The bottom positioning member 2 has the same structure as the bottom positioning member 1.

[0009] According to the above technical solution, the conveying member includes servo motor 1, servo motor 2, threaded rod 1, threaded rod 2, conveying block, end plate 1 and end plate 2, the servo motor 1 and the servo motor 2 are respectively movably arranged at one end adjacent to the make way area through a slide groove, the output ends of the servo motor 1 and the servo motor 2 are respectively fixedly installed with the threaded rod 1 and the threaded rod 2, the threaded rod 1 and the threaded rod 2 are respectively arranged in a cross-staggered manner and the conveying block is commonly threadedly connected in the middle, the ends of the threaded rod 1 and the threaded rod 2 are respectively fixedly installed with the end plate 1 and the end plate 2, and the controller is respectively provided with wireless communication connection with the servo motor 1 and the servo motor 2.

[0010] According to the above technical solution, the bottom positioning member 1 includes an electric telescopic rod, a support plate and a positioning ring. The electric telescopic rod is fixedly installed at the bottom of the positioning groove, the support plate is fixedly installed on the telescopic end of the electric telescopic rod, the positioning ring is fixedly installed on the upper end of the support plate, the lower end of the culture bottle is provided with a bottom groove corresponding to the positioning ring, and a wireless communication connection is set between the controller and the electric telescopic rod.

[0011] According to the above technical solution, the picking and placing component includes a multi-stage cylinder three, an arc plate and an electromagnet. The multi-stage cylinder three is fixedly installed in the corresponding placement space, the arc plate is fixedly installed on the telescopic end of the multi-stage cylinder three, the electromagnet is fixedly installed on the arc surface inside the arc plate, a metal ring corresponding to the electromagnet is arranged on the outside of the culture bottle, and wireless communication connections are respectively set between the controller and the multi-stage cylinder three and the electromagnet.

[0012] According to the above technical solution, the temperature control unit includes a temperature control board 1, a temperature sensor 1, a temperature control board 2 and a temperature sensor 2. The temperature control board 1 is embedded and installed on the upper end of the bottom plate for controlling the temperature of the micro-control space. The temperature sensor 1 is fixedly installed on the upper end of the bottom plate for monitoring the temperature of the micro-control space. An isolation groove is provided at one end of the culture space and adjacent to the central channel. A controllable isolation part is provided in the isolation groove for separately isolating and sealing the culture space. The temperature control board 2 is fixedly installed on the inner wall of the culture space for controlling the temperature of the culture space. The temperature sensor 2 is fixedly installed on the top wall of the culture space for monitoring the temperature of the culture space. Wireless communication connections are respectively set between the controller and the temperature control board 1, the temperature sensor 1, the temperature control board 2 and the temperature sensor 2. An air inlet pipe and an air outlet pipe are respectively provided at one end of the culture space.

[0013] According to the above technical solution, the controllable isolation part includes a servo motor three, a threaded rod three and an isolation plate. The servo motor three is fixedly installed at the bottom of the isolation groove. The output end of the servo motor three is fixedly installed on the threaded rod three. The threaded rod three is threadedly connected with the isolation plate. The isolation plate is movably installed in the corresponding isolation groove. A wireless communication connection is set between the controller and the servo motor three.

[0014] According to the above technical solution, the controller includes a control seat, a touch screen and a button control area. The control seat is movably installed at the central position of the upper end of the sealing cover and a handle is fixedly installed at the front end of the control seat. The touch screen is obliquely arranged on the control seat, and a button control area is provided at the lower end of the touch screen.

[0015] A cell culture method comprising the following steps: S1, pre-adjustment and identification: placing the culture bottle into the micro-controlled space, quickly adjusting the temperature of the micro-controlled space by the temperature control unit to adapt the culture bottle to the target temperature, and obtaining the identity information and cell type data of the culture bottle by the RFID card reader; S2, zero-contact grasping: the electromagnet absorbs the metal ring on the outer wall of the culture bottle to transfer the culture bottle from the micro-controlled space to the conveying channel; S3, precise positioning and transportation: using the cross-shaped threaded rod 1 and the cross-shaped threaded rod 2 to control the movement of the conveying block, and pushing the culture bottle along the conveying channel to the designated position of the culture area; S4, independent temperature control and isolation: the culture area is sealed by the isolation plate, the temperature control plate 2 is started to maintain the set temperature of the culture area, and whether to supply gas is selected according to the cell type; S5, data monitoring and feedback: real-time collection of the temperature data of the culture area and the identity information of the culture bottle, and data storage and remote transmission through the controller; S6, culture termination and recovery: After the culture is completed, steps S2-S3 are performed in reverse, and the culture bottle is moved out of the culture area and taken out.

[0016] Compared with the prior art, the beneficial effects achieved by the present invention are: (1) Efficient space utilization: The culture space and placement space are distributed in a circular array along the central channel, maximizing the use of the internal space of the incubator and supporting the parallel culture of multiple batches and multiple types of cells; (2) Modular culture area: Each culture area is independently controllable, allowing experiments under different temperature conditions (such as 37°C mammalian cell culture and 25°C insect cell culture) to be carried out simultaneously, significantly improving experimental throughput; (3) Pre-conditioning in micro-controlled space: The independent space formed by the seal and the sealing cover, combined with the temperature control board, quickly adjusts the temperature of the newly placed culture bottle to avoid temperature fluctuations or contamination of the main culture environment. The culture bottle is completely isolated during the pre-conditioning stage to prevent external microorganisms from contaminating the main culture environment and reduce the risk of contamination; (4) Independent isolation and temperature control: The controllable isolation parts can close the culture area by lifting the isolation plate, and combined with the temperature control plate 2, the differentiated temperature control of each area can be achieved to avoid cross interference; (5) Automated transfer system: The metal ring of the culture bottle is adsorbed by an electromagnet, and a multi-stage cylinder 3 is used to achieve contactless grasping, reducing the risk of human contamination. The cross-threaded rod composed of threaded rod 1 and threaded rod 2 drives the conveying block to accurately position, ensuring that the culture bottle accurately enters the positioning groove, reducing the need for manual intervention and improving work efficiency. (6) Wireless collaborative control: The controller integrates a wireless communication module to remotely control multi-stage cylinders, servo motors and temperature control units, reducing manual intervention and improving operational efficiency; (7) RFID full life cycle tracking: RFID card reader records the batch, culture time, temperature curve and other data of the culture bottle to support experimental traceability and quality control; (8) Remote monitoring and alarm: The controller displays the temperature and humidity data of each area in real time through the touch screen, triggers an alarm when an abnormality occurs (such as temperature exceeding the limit, communication interruption), and supports cloud data backup. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings: Figure 1 is a first stereoscopic schematic diagram of the present invention; Figure 2 is a second stereoscopic schematic diagram of the present invention; Figure 3 is a first partial stereoscopic schematic diagram of the present invention; Figure 4 is a second partial stereoscopic schematic diagram of the present invention; Figure 5 is a third partial stereoscopic schematic diagram of the present invention; Figure 6 is a fourth partial stereoscopic schematic diagram of the present invention; Figure 7 is a fifth partial stereoscopic schematic diagram of the present invention; Figure 8 is a sixth partial stereoscopic schematic diagram of the present invention; Fig. 9 is a seventh partial stereoscopic schematic diagram of the present invention; Fig.10is an eighth partial stereoscopic schematic diagram of the present invention; Fig.11 is a ninth partial stereoscopic schematic diagram of the present invention; Fig.12 The present invention Fig.11 The enlarged schematic diagram of point A in the middle; In the figure: 1-cell culture box, 11-central channel, 12-sealing cover, 13-controller, 131-control seat, 132-touch screen, 133-button control area, 14-multi-stage cylinder one, 15-RFID reader, 16-culture space, 161-transport channel, 162-culture area, 163-allowance area, 164-transport frame, 165-transport trough, 166-isolation trough, 167-positioning groove, 17-placing space, 18-inlet pipe, 19-outlet pipe, 2-culture bottle, 21-metal ring, 3-seal, 31-bottom plate, 311-positioning groove, 32-annular side plate, 33- L-shaped plate, 34-multi-stage cylinder two, 35-bottom positioning part one, 351-electric telescopic rod, 352-support plate, 353-positioning ring, 4-pick-and-place part, 41-multi-stage cylinder three, 42-arc plate, 43-electromagnet, 5-temperature control part, 51-temperature control board one, 52-temperature sensor one, 53-temperature control board two, 54-temperature sensor two, 6-conveying part, 61-servo motor one, 62-servo motor two, 63-threaded rod one, 64-threaded rod two, 65-conveying block, 66-end plate one, 67-end plate two, 7-controllable isolation part, 71-servo motor three, 72-threaded rod three, 73-isolation plate. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] See also Figure 1-12The present invention provides a technical solution: a cell culture device, including a cell culture box 1 and a culture bottle 2, wherein a central channel 11 is provided at the central position of the cell culture box 1, an opening is provided at the upper end of the central channel 11, and a sealing cover 12 is threadedly connected to the opening, a take-in and put-out port is provided at the middle position of the sealing cover 12, and a controller 13 is movably installed in the take-in and put-out port, a multi-stage cylinder 14 is fixedly installed at the bottom of the central channel 11, a sealing member 3 is provided at the top of the multi-stage cylinder 14, and an RFID reader 15 is provided on the sealing member 3, a micro-control space is formed between the sealing member 3 and the sealing cover 12, and the RFID reader 15 is used to identify the culture bottle 2, and the The controller 13 is respectively connected to the multi-stage cylinder 14 and the RFID card reader 15 by wireless communication. The inner wall of the cell culture box 1 is respectively provided with a culture space 16 and a placement space 17. The culture space 16 and the placement space 17 are relatively arranged on both sides of the central channel 11. The culture space 16 and the placement space 17 are arranged in a linear array along the cell culture box 1 and in a circumferential array at the same level. A pick-and-place component 4 is arranged in the placement space 17 for taking and placing the culture bottle 2 into the corresponding culture space 16 for placement and culture. A temperature control component 5 is arranged in the cell culture box 1, and the temperature control component 5 controls the temperature of the micro-control space and the cell culture box 1 respectively. The central channel 11 is located at the center of the cell culture incubator 1. The top opening is connected to the sealing cover 12 through a thread to ensure the closedness of the internal environment. A take-in and put-out port is provided in the middle of the sealing cover 12, and a built-in controller 13 is used to control and monitor the operation of the equipment. The micro-control space is formed by the sealing member 3 and the sealing cover 12. It is a space independent of the interior of the incubator 1 and is specifically used for rapid temperature control and preliminary treatment of the newly placed culture bottles 2. The temperature in the micro-control space can be quickly adjusted so that the newly placed culture bottles can quickly adapt to the environmental conditions inside the incubator while avoiding leakage and contamination of the internal culture environment. The multi-stage cylinder 14 is fixedly installed at the bottom of the central channel 11 and can be extended to lift or lower the sealing member 3. The sealing member 3 is equipped with an RFID reader 15 for identifying the placed culture bottles 2. The RFID reader 15 is used to identify and scan the culture bottles 2 and facilitate subsequent tracking and management. The data integrated management system can record each culture bottle 2 The controller 13 connects and controls the multi-stage cylinder 1, the RFID card reader and the pick-and-place unit 4 through wireless communication to realize automatic management and operation. The culture space 16 and the placement space 17 are relatively arranged on both sides of the central channel 11, and are arranged in a circular array to maximize the use of space. The culture space 16 is the only location for storing and culturing cells. It is designed as a linear array and a circular array layout to facilitate efficient use of space and convenient management. The placement space 17 is specially designed to provide operation space for the pick-and-place unit 4 and does not have a storage function. The pick-and-place unit 4 is located in the placement space 17 and is responsible for moving the culture bottle 2 from one position to another, that is, pushing it from the micro-control space to the designated position in the culture space 16 for cultivation. The temperature control unit 5 controls the temperature of the entire cell culture box 1 and the micro-control space to ensure the best growth environment. Specifically, the sealing member 3 includes a bottom plate 31, an annular side plate 32, an L-shaped plate 33, a multi-stage cylinder 34 and a bottom positioning member 35. The bottom plate 31 is fixedly mounted on the telescopic end of the multi-stage cylinder 14 and the side wall of the bottom plate 31 is movably sleeved with the annular side plate 32. The multi-stage cylinder 34 is fixedly mounted on the edge of the lower end of the bottom plate 31 and the L-shaped plate 33 is fixedly mounted on the telescopic end of the multi-stage cylinder 34. The other end of the L-shaped plate 33 is fixedly mounted on the lower end of the annular side plate 32. A positioning groove 311 is provided at the middle position of the upper end of the bottom plate 31. The bottom positioning member 35 is located in the positioning groove 311. The bottom positioning member 35 is used to perform bottom positioning of the culture bottle 2. The RFID card reader 15 is located on the bottom positioning member 35. A wireless communication connection is set between the controller 13 and the multi-stage cylinder 34. The bottom plate 31 is fixedly mounted on the telescopic end of the multi-stage cylinder 14 as the basic support part of the entire sealing member 3. A positioning groove 311 is provided in the middle of the bottom plate 31 for placing the bottom positioning member 1 35. The annular side plate 32 is movably sleeved on the side wall of the bottom plate 31 and can move up and down with the bottom plate 31. The L-shaped plate 33 is connected to the bottom plate 31 through the multi-stage cylinder 2 34. The multi-stage cylinder 2 34 is fixedly mounted on the lower edge of the bottom plate 31, and the telescopic end thereof is fixedly mounted with the L-shaped plate 33. The other end of the L-shaped plate 33 is fixedly mounted on the lower end of the annular side plate 32. The position adjustment of the annular side plate 32 is controlled by the telescopic action of the multi-stage cylinder 2 34 to ensure The annular side plate 32 can fit tightly with the bottom plate 31 to form a closed space. The multi-stage cylinder 2 34 is responsible for controlling the up and down movement of the L-shaped plate 33 and the annular side plate 32 to realize the opening and closing control of the micro-control space and realize the adjustable sealing height. A wireless communication connection is set between the multi-stage cylinder 2 and the controller 13 to facilitate remote control and automatic operation. The bottom positioning member 1 35 is located in the positioning groove 311 at the middle position of the upper end of the bottom plate 31, which is used to position the bottom of the culture bottle 2 to ensure the stability and accuracy of the culture bottle 2 in the micro-control space. The positioning groove 311 cooperates with the RFID card reader 15 to realize the simultaneous completion of bottle body fixing and data reading. Specifically, the culture space 16 includes a conveying channel 161, a culture area 162 and a yield area 163. The conveying channel 161 is connected to the central channel 11. The culture areas 162 are arranged on both sides of the conveying channel 161 in a mirror-symmetrical manner. A conveying frame 164 is arranged in a mirror-symmetrical manner in the conveying channel 161. A conveying groove 165 is commonly provided at opposite ends of the conveying frame 164. The conveying frame 164 extends into the culture area 162 and is provided with a positioning groove 167. The positioning groove 167 is used to position and place the culture bottle 2 for culture. The yield area 163 is provided at the bottom of the conveying channel 161. A conveying member 6 is provided in the yield area 163. The conveying member 6 is used to position and convey the culture bottle 2. A bottom positioning member 2 is provided on the conveying member 6. The bottom positioning member 2 and the bottom positioning member 1 35 have the same structure. The conveying channel 161 is connected to the central channel 11, and serves as the main path for the culture bottle 2 to enter the culture area 162. The culture area 162 is arranged on both sides of the conveying channel 161 in a mirror-symmetrical manner, providing multiple positions for placing and culturing cells. The conveying frame 164 is arranged in the conveying channel 161 in a mirror-symmetrical manner. A conveying groove 165 is provided at the opposite end of each side frame for guiding and supporting the culture bottle 2. The conveying frame 164 extends into the culture area 162, and a positioning groove 167 is provided thereon for accurately positioning and placing the culture bottle. The clearance area 163 is located at the bottom of the conveying channel 161, providing an operating space for the conveying member 6 to ensure that the culture bottle 2 can be smoothly moved from the conveying channel 161 to the designated culture position. The conveying member 6 is located in the clearance area 163, and is responsible for conveying the culture bottle 2 from the conveying channel 161 to the designated position in the culture area 162. The conveying member 6 is provided with a bottom positioning member 2, and its structure and function are the same as the bottom positioning member 1 35 in the sealing member 3, and is used to ensure the stability and accuracy of the culture bottle 2 during the conveying process. Specifically, the conveying member 6 includes a servo motor 1 61, a servo motor 2 62, a threaded rod 1 63, a threaded rod 2 64, a conveying block 65, an end plate 1 66 and an end plate 2 67. The servo motor 1 61 and the servo motor 2 62 are respectively movably arranged at one end adjacent to the yielding area 163 through a slide groove. The output ends of the servo motor 1 61 and the servo motor 2 62 are respectively fixedly installed with the threaded rod 1 63 and the threaded rod 2 64. The threaded rod 1 63 and the threaded rod 2 64 are cross-staggered and the conveying block 65 is commonly threadedly connected in the middle. The ends of the threaded rod 1 63 and the threaded rod 2 64 are respectively fixedly installed with the end plate 1 66 and the end plate 2 67. The controller 13 is respectively provided with wireless communication connection with the servo motor 1 61 and the servo motor 2 62; The servo motor 1 61 and the servo motor 2 62 are respectively arranged at one end adjacent to the yielding area 163 through a slide slot, providing a power source to drive the threaded rod 1 63 and the threaded rod 2 64. The output ends of the servo motor 1 61 and the servo motor 2 62 are respectively fixed with the threaded rod 1 63 and the threaded rod 2 64, and are connected to the controller 13 through wireless communication to realize automatic control. The threaded rod 1 63 and the threaded rod 2 64 are arranged in a cross-staggered manner, and a conveying block 65 is commonly threadedly connected in the middle to form a precise two-dimensional moving system. This design makes the conveying The delivery block 65 can move precisely in two mutually perpendicular directions, so as to realize precise positioning and delivery of the culture bottle 2. The delivery block 65 is connected to the threaded rod 1 63 and the threaded rod 2 64 by threads, and can move precisely along these two directions. The delivery block 65 is provided with a bottom positioning member 2, which is used to ensure the stability and accuracy of the culture bottle during the delivery process. The ends of the threaded rod 1 63 and the threaded rod 2 64 are respectively fixedly installed with an end plate 1 66 and an end plate 2 67, which play the role of limiting and supporting, and ensure the stability of the threaded rod; Specifically, the bottom positioning member 35 includes an electric telescopic rod 351, a support plate 352 and a positioning ring 353. The electric telescopic rod 351 is fixedly installed at the bottom of the positioning groove 311. The support plate 352 is fixedly installed at the telescopic end of the electric telescopic rod 351. The positioning ring 353 is fixedly installed at the upper end of the support plate 352. The lower end of the culture bottle 2 is provided with a bottom groove corresponding to the positioning ring 353. A wireless communication connection is set between the controller 13 and the electric telescopic rod 351. The electric telescopic rod 351 is fixedly mounted on the bottom of the positioning groove 311 on the bottom plate 31, providing power to realize the up and down movement of the support plate 352. The electric telescopic rod 351 is connected to the controller 13 through wireless communication, and can receive instructions for precise height adjustment. The support plate 352 is fixedly mounted on the telescopic end of the electric telescopic rod 351, and moves up and down with the telescopic action of the electric telescopic rod 351. The support plate 352 is used to carry and support the positioning ring 353 to ensure that the positioning ring 353 can stably position the culture bottle 2. The positioning ring 353 is fixedly mounted on the upper end of the support plate 352, and is used to accurately position the bottom of the culture bottle 2. The lower end of the culture bottle 2 is provided with a bottom groove corresponding to the positioning ring 353, ensuring that the culture bottle 2 can be accurately placed and kept stable; Specifically, the pick-and-place member 4 includes a multi-stage cylinder 3 41, an arc plate 42 and an electromagnet 43. The multi-stage cylinder 3 41 is fixedly installed in the corresponding placement space 17. The arc plate 42 is fixedly installed on the telescopic end of the multi-stage cylinder 3 41. The electromagnet 43 is fixedly installed on the inner arc surface of the arc plate 42. A metal ring 21 corresponding to the electromagnet 43 is arranged on the outside of the culture bottle 2. The controller 13 is respectively connected to the multi-stage cylinder 3 41 and the electromagnet 43 through wireless communication. The multi-stage cylinder 3 41 is fixedly installed in the corresponding placement space 17, providing power to realize the up and down movement of the arc plate 42. The multi-stage cylinder 3 41 is connected to the controller 13 through wireless communication, and can receive instructions for precise height adjustment. The arc plate 42 is fixedly installed on the telescopic end of the multi-stage cylinder 3 41, and moves up and down with the telescopic action of the multi-stage cylinder 3 41. The arc plate 42 is used to carry and support the electromagnet 43 to ensure that the electromagnet 43 can stably grasp and release the culture bottle 2. The electromagnet 43 is fixedly installed on the inner arc surface of the arc plate 42, and is used to grasp and release the culture bottle 2. A metal ring 21 corresponding to the electromagnet 43 is arranged on the outside of the culture bottle 2. When the electromagnet 43 is energized, the metal ring 21 can be adsorbed by magnetic force, thereby realizing the grasping of the culture bottle 2. When the power is off, the culture bottle 2 is released. The electromagnet 43 adsorbs the external metal ring 21 of the culture bottle 2, which can avoid direct contact with the bottle body and perform non-contact grasping. Specifically, the temperature control unit 5 includes a temperature control board 1 51, a temperature sensor 1 52, a temperature control board 2 53 and a temperature sensor 2 54. The temperature control board 1 51 is embedded in the upper end of the bottom plate 31 for controlling the temperature of the micro-control space. The temperature sensor 1 52 is fixedly installed on the upper end of the bottom plate 31 for monitoring the temperature of the micro-control space. An isolation groove 166 is provided in the culture space 16 and at one end adjacent to the central channel 11. A controllable isolation member 7 is provided in the isolation groove 166 for controlling the temperature of the culture space. 16 is separately isolated and sealed, the temperature control board 2 53 is fixedly installed on the inner wall of the culture space 16 for controlling the temperature of the culture space 16, the temperature sensor 2 54 is fixedly installed on the top wall of the culture space 16 for monitoring the temperature of the culture space 16, the controller 13 is respectively connected with the temperature control board 1 51, the temperature sensor 1 52, the temperature control board 2 53 and the temperature sensor 2 54 by wireless communication, and one end of the culture space 16 is respectively provided with an air inlet pipe 18 and an air outlet pipe 19; The heating mode of the temperature control board 1 51 and the temperature control board 2 53 is set to a thin-film PTC heating plate, and the semiconductor cooling plate is integrated on one side of the temperature control board to support active cooling. The temperature sensor 1 52 and the temperature sensor 2 54 use high-precision NTC thermistors. The isolation groove 166 is opened in the culture space 16 and is adjacent to one end of the central channel 11, which is used to accommodate the controllable isolation member 7. The controllable isolation member 7 can be used to isolate and seal the culture space 16 separately as needed to prevent temperature interference between different culture areas 162, and ensure that the temperature of each area is independently controlled. Different temperatures can be set for each culture area 162, such as 37°C for mammalian cells and 25°C for insect cells, without interfering with each other. An air inlet pipe 18 and an air outlet pipe 19 are set on one side of the culture space 16 to transport the required gas to the culture area 162 and connect to an external gas supply device. Control valves are installed on these pipes. The control valves are controlled by the controller 13 and can automatically adjust the input and discharge of gas according to different cell types (such as mammalian cells or insect cells); Specifically, the controllable isolation member 7 includes a servo motor 3 71, a threaded rod 3 72 and an isolation plate 73. The servo motor 3 71 is fixedly mounted on the bottom of the isolation groove 166. The output end of the servo motor 3 71 is fixedly mounted on the threaded rod 3 72. The threaded rod 3 72 is threadedly connected with the isolation plate 73. The isolation plate 73 is movably mounted in the corresponding isolation groove 166. A wireless communication connection is set between the controller 13 and the servo motor 3 71. The servo motor 3 71 is fixedly installed at the bottom of the isolation groove 166, providing power to drive the threaded rod 3 72 to rotate. The servo motor 3 71 is connected to the controller 13 through wireless communication, and can receive instructions for precise control. The isolation plate 73 is movably installed in the corresponding isolation groove 166 and is connected to the threaded rod 3 72 through threads. The isolation plate can move up and down under the drive of the servo motor 3 71, thereby realizing the sealing or opening operation of the culture space, ensuring the independent control of the temperature between different culture areas 162; Specifically, the controller 13 includes a control seat 131, a touch screen 132 and a button control area 133. The control seat 131 is movably mounted at the center of the upper end of the sealing cover 12 and a handle is fixedly mounted at the front end of the control seat 131. The touch screen 132 is tiltedly arranged on the control seat 131, and the button control area 133 is arranged at the lower end of the touch screen 132. The control seat 131 is movably mounted at the center of the upper end of the sealing cover 12, and a handle is fixedly mounted at the front end to facilitate manual operation or movement by the operator. The control seat 131 is the main supporting part of the entire controller 13, and various electronic components and communication modules are integrated inside to process and transmit instructions. It is connected to the sealing cover 12 through magnetic contacts, and supports one-handed disassembly and maintenance. The back is provided with heat dissipation fins and a fan to ensure that it does not overheat during long-term operation. The touch screen 132 is tilted on the control seat 131 to facilitate viewing and operation by the operator. The touch screen 132 provides a graphical user interface, allowing the user to select and adjust various parameters, view real-time data, monitor equipment status, etc. by touching the screen. The screen can display a variety of information such as temperature, culture bottle status, operation progress, etc., providing an intuitive operating experience. The button control area 133 is located below the touch screen 132 and includes multiple physical buttons for quickly executing common operations or manual intervention in emergency situations. These buttons include a start / stop button, an emergency stop button, a mode switching button, etc., to ensure rapid response when needed; A cell culture method comprising the following steps: S1, Preconditioning and Identification S1.1, place the culture bottle 2 containing the cell sample into the micro-controlled space, and quickly adjust the temperature of the micro-controlled space through the temperature control module (temperature control unit 5) so that the culture bottle 2 quickly adapts to the target temperature.

[0020] S1.2, at the same time, the identification module (RFID reader 15) obtains the identity information and cell type data of the culture bottle 2, and uploads the information to the controller 13.

[0021] S2, zero-touch gripping S2.1, using the magnetic suction component (electromagnet 43) to absorb the metal ring 21 on the outer wall of the culture bottle 2 to achieve zero-contact grasping.

[0022] S2.2, control the multi-stage cylinder 3 41 to drive the arc plate 42 and the electromagnet 43 to transfer the culture bottle 2 from the micro-control space to the conveying channel 161.

[0023] S3, precise positioning and conveying S3.1, using a two-dimensional driving mechanism (crossed threaded rod 1 63, threaded rod 2 64) to control the conveying block 65 to move precisely in two mutually perpendicular directions.

[0024] S3.2, push the culture bottle 2 along the conveying channel 161 to the designated position of the culture area 162, and ensure that the culture bottle 2 is accurately placed in the positioning groove 167.

[0025] S4, independent temperature control and isolation S4.1, the culture area 162 is closed by a lifting isolation plate 73 to prevent temperature interference between different culture areas 162.

[0026] S4.2, start the independent temperature control module (temperature control board 2 53) to maintain the set temperature of the culture area 162 to ensure that the cells grow in the best environment.

[0027] S4.3, if the cells are mammalian cells, open the control valves on the inlet pipe 18 and outlet pipe 19 of the culture area 162 to deliver 5% CO into the culture area 162. 2 If the mixed gas is insect cells, keep the control valves of the air inlet pipe 18 and the air outlet pipe 19 closed.

[0028] S5, Data Monitoring and Feedback S5.1, collect temperature data of the culture area 162 and identity information of the culture bottle 2 in real time, and store the data through the controller 13.

[0029] S5.2, use networking functions to achieve remote transmission and ensure the security and traceability of experimental data.

[0030] S6, culture termination and recovery S6.1, after the culture is completed, the steps S2-S3 are performed in reverse, and the metal ring 21 on the outer wall of the culture bottle 2 is re-adsorbed by the magnetic suction component (electromagnet 43), and it is removed from the culture area 162 and returned to the delivery channel 161.

[0031] S6.2, finally taking out the culture bottle 2 from the delivery channel 161 for subsequent processing (such as harvesting cells, analyzing results, etc.).

[0032] Working principle: This application is suitable for differentiated temperature control requirements of mammalian cells, insect cells, etc. Through the cell culture box 1, controller 13, seal 3, pick-up and placement part 4, conveyor 6 and other components, the controller 13 is connected to the multi-stage cylinder, servo motor, temperature control unit and sensor through wireless communication to realize full-process automated operation, and cell culture is carried out through a multi-stage linkage process: placing the culture bottle 2, pre-adjusting the temperature of the micro-controlled space, RFID identification, the seal 3 descending, the pick-up and placement part 4 grabbing, the conveyor 6 positioning, the isolation plate 73 closing the culture area 162, independent temperature control and reverse operation after the culture is completed to remove the culture bottle 2, realize automated operation, simplify the process, and lower the operating threshold of the experimenter. The culture area 162 distributed in a circular array supports parallel cultivation of multiple batches, has high space utilization, and the micro-controlled space is quickly pre-adjusted. The culture area 162 is independently temperature controlled to avoid cross interference. RFID tracking and remote monitoring realize full life cycle data traceability. This application integrates RFID information tracking, wireless communication and independent temperature control modules to realize autonomous operation of the entire process from placement, pre-adjustment, positioning to isolated culture, and realize closed-loop intelligent control of cell culture.

[0033] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0034] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A cell culture device, comprising a cell culture box (1) and a culture bottle (2), characterized in that: A central channel (11) is provided at the central position of the cell culture box (1), an opening is provided at the upper end of the central channel (11), and a sealing cover (12) is threadedly connected to the opening, a take-in and put-out opening is provided at the middle position of the sealing cover (12), and a controller (13) is movably installed in the take-in and put-out opening, a multi-stage cylinder (14) is fixedly installed at the bottom of the central channel (11), a sealing member (3) is provided at the top of the multi-stage cylinder (14), and an RFID card reader (15) is provided on the sealing member (3), a micro-control space is formed between the sealing member (3) and the sealing cover (12), and the RFID card reader (15) is used to identify the culture bottle (2), and the controller (13) is respectively connected to the multi-stage cylinder (14) and the RFID card reader (15). A wireless communication connection is set between the cell culture box (1) and the RFID card reader (15); the inner side walls of the cell culture box (1) are respectively provided with a culture space (16) and a placement space (17); the culture space (16) and the placement space (17) are arranged on both sides of the central channel (11) relative to each other; the culture space (16) and the placement space (17) are arranged in a linear array along the cell culture box (1) and in a circular array at the same level; a pick-and-place component (4) is provided in the placement space (17) for taking and placing the culture bottle (2) into the corresponding culture space (16) for placement and culture; a temperature control component (5) is provided in the cell culture box (1); the temperature control component (5) controls the temperature of the micro-control space and the cell culture box (1) respectively.

2. A cell culture device according to claim 1, characterized in that: The sealing member (3) comprises a bottom plate (31), an annular side plate (32), an L-shaped plate (33), a multi-stage cylinder 2 (34) and a bottom positioning member 1 (35); the bottom plate (31) is fixedly mounted on the telescopic end of the multi-stage cylinder 1 (14) and the annular side plate (32) is movably sleeved on the side wall of the bottom plate (31); the multi-stage cylinder 2 (34) is fixedly mounted on the lower edge of the bottom plate (31) and the L-shaped plate (33) is fixedly mounted on the telescopic end of the multi-stage cylinder 2 (34); The other end of the L-shaped plate (33) is fixedly mounted on the lower end of the annular side plate (32); a positioning groove (311) is provided at the middle position of the upper end of the bottom plate (31); the bottom positioning member 1 (35) is located in the positioning groove (311); the bottom positioning member 1 (35) is used to perform bottom positioning on the culture bottle (2); the RFID card reader (15) is located on the bottom positioning member 1 (35); and a wireless communication connection is provided between the controller (13) and the multi-stage cylinder 2 (34).

3. A cell culture device according to claim 2, characterized in that: The culture space (16) comprises a conveying channel (161), a culture area (162) and a clearance area (163); the conveying channel (161) is connected to the central channel (11); the culture area (162) is arranged on both sides of the conveying channel (161) in a mirror-symmetrical manner; a conveying frame (164) is arranged in a mirror-symmetrical manner in the conveying channel (161); opposite ends of the conveying frame (164) are provided with a conveying groove (165); the conveying frame (164) extends The conveying member (162) extends into the culture area (162) and is provided with a positioning groove (167), wherein the positioning groove (167) is used to position and place the culture bottle (2) for culture. The yielding area (163) is provided at the bottom of the conveying channel (161), wherein a conveying member (6) is provided in the yielding area (163), wherein the conveying member (6) is used to position and convey the culture bottle (2), wherein a bottom positioning member 2 is provided on the conveying member (6), wherein the bottom positioning member 2 and the bottom positioning member 1 (35) have the same structure.

4. A cell culture device according to claim 3, characterized in that: The conveying member (6) comprises a servo motor 1 (61), a servo motor 2 (62), a threaded rod 1 (63), a threaded rod 2 (64), a conveying block (65), an end plate 1 (66) and an end plate 2 (67); the servo motor 1 (61) and the servo motor 2 (62) are respectively movably arranged at one end adjacent to the yielding area (163) through a slide groove; the threaded rod 1 (63) and the threaded rod 2 (64) are respectively fixedly mounted at the output ends of the servo motor 1 (61) and the servo motor 2 (62); the threaded rod 1 (63) and the threaded rod 2 (64) are arranged in a cross-staggered manner and the conveying block (65) is commonly threadedly connected in the middle; the end plates 1 (66) and the end plates 2 (67) are respectively fixedly mounted at the ends of the threaded rod 1 (63) and the threaded rod 2 (64); and the controller (13) is respectively provided with wireless communication connections with the servo motor 1 (61) and the servo motor 2 (62).

5. A cell culture device according to claim 3, characterized in that: The bottom positioning member 1 (35) comprises an electric telescopic rod (351), a support plate (352) and a positioning ring (353); the electric telescopic rod (351) is fixedly mounted on the bottom of the positioning groove (311); the support plate (352) is fixedly mounted on the telescopic end of the electric telescopic rod (351); the positioning ring (353) is fixedly mounted on the upper end of the support plate (352); a bottom groove corresponding to the positioning ring (353) is provided at the lower end of the culture bottle (2); and a wireless communication connection is arranged between the controller (13) and the electric telescopic rod (351).

6. A cell culture device according to claim 1, characterized in that: The pick-and-place component (4) comprises a multi-stage cylinder three (41), an arc plate (42) and an electromagnet (43); the multi-stage cylinder three (41) is fixedly installed in the corresponding placement space (17); the arc plate (42) is fixedly installed on the telescopic end of the multi-stage cylinder three (41); the electromagnet (43) is fixedly installed on the inner arc surface of the arc plate (42); a metal ring (21) corresponding to the electromagnet (43) is arranged on the outside of the culture bottle (2); and wireless communication connections are respectively set between the controller (13) and the multi-stage cylinder three (41) and the electromagnet (43).

7. A cell culture device according to claim 2, characterized in that: The temperature control unit (5) comprises a temperature control board 1 (51), a temperature sensor 1 (52), a temperature control board 2 (53) and a temperature sensor 2 (54); the temperature control board 1 (51) is embedded and installed on the upper end of the base plate (31) for controlling the temperature of the micro-controlled space; the temperature sensor 1 (52) is fixedly installed on the upper end of the base plate (31) for monitoring the temperature of the micro-controlled space; an isolation groove (166) is provided in the culture space (16) at one end adjacent to the central channel (11); a controllable isolation member (7) is provided in the isolation groove (166) for controlling the temperature of the culture space (16) The culture space (16) is isolated and sealed separately. The second temperature control plate (53) is fixedly mounted on the inner wall of the culture space (16) for controlling the temperature of the culture space (16). The second temperature sensor (54) is fixedly mounted on the inner top wall of the culture space (16) for monitoring the temperature of the culture space (16). The controller (13) is respectively connected to the first temperature control plate (51), the first temperature sensor (52), the second temperature control plate (53) and the second temperature sensor (54). An air inlet pipe (18) and an air outlet pipe (19) are respectively arranged at one end of the culture space (16).

8. A cell culture device according to claim 7, characterized in that: The controllable isolation member (7) comprises a servo motor three (71), a threaded rod three (72) and an isolation plate (73); the servo motor three (71) is fixedly mounted on the bottom of the isolation groove (166); an output end of the servo motor three (71) is fixedly mounted on the threaded rod three (72); the threaded rod three (72) is threadedly connected to the isolation plate (73); the isolation plate (73) is movably mounted in the corresponding isolation groove (166); and a wireless communication connection is provided between the controller (13) and the servo motor three (71).

9. A cell culture device according to claim 1, characterized in that: The controller (13) comprises a control seat (131), a touch screen (132) and a button control area (133); the control seat (131) is movably mounted at the central position of the upper end of the sealing cover (12) and a handle is fixedly mounted at the front end of the control seat (131); the touch screen (132) is obliquely arranged on the control seat (131), and the button control area (133) is arranged at the lower end of the touch screen (132).

10. A cell culture method according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1, pre-adjustment and identification: placing the culture bottle (2) into the micro-controlled space, quickly adjusting the temperature of the micro-controlled space by the temperature control unit (5) so that the culture bottle (2) adapts to the target temperature, and obtaining the identity information and cell type data of the culture bottle (2) by the RFID card reader (15); S2, zero-contact grasping: the electromagnet (43) adsorbs the metal ring (21) on the outer wall of the culture bottle (2), thereby transferring the culture bottle (2) from the micro-controlled space to the transport channel (161); S3, precise positioning and conveying: using the cross-shaped threaded rod 1 63 and the cross-shaped threaded rod 2 64 to control the movement of the conveying block (65), and pushing the culture bottle (2) along the conveying channel (161) to a designated position in the culture area (162); S4, independent temperature control and isolation: the culture area (162) is sealed by the isolation plate (73), the temperature control plate 2 53 is started to maintain the set temperature of the culture area (162), and whether to supply gas is selected according to the cell type; S5, data monitoring and feedback: collecting temperature data of the culture area (162) and identity information of the culture bottle (2) in real time, and performing data storage and remote transmission through a controller (13); S6, culture termination and recovery: After the culture is completed, steps S2-S3 are performed in reverse, and the culture bottle (2) is moved out of the culture area (162) and taken out.

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