White rot fungi WH-B-X2 used as soil biological modifier, application of white rot fungi WH-B-X2, soil biological modifier and preparation method of soil biological modifier

Abstract

The invention provides white rot fungi WH-B-X2 used as a soil biological modifier, application of the white rot fungi WH-B-X2, the soil biological modifier and a preparation method of the soil biological modifier, and belongs to the technical field of biology. The white rot fungus WH-B-X2 is preserved in China Center for Type Culture Collection on May 8, 2024, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 2024883. The soil biological improver provided by the embodiment of the invention has a good inhibition effect on effective cadmium in soil, can remarkably reduce the cadmium content in crops, and meanwhile, can improve the pH value of the soil, so that the pH value of the soil can be suitable for growth and development of the crops, and the soil is prevented from being too acidic.

Description

Technical Field

[0001] The present disclosure relates to the field of biotechnology, and in particular to a white rot fungus WH-B-X2 used as a soil biomodifier and an application thereof, a soil biomodifier and a preparation method thereof. Background Art

[0002] With the rapid development of urbanization and industrialization, the problem of heavy metal pollution in soil has become increasingly serious and has become an important environmental problem that needs to be solved urgently. Heavy metals have the characteristics of long residual period, irreversibility, easy migration and high toxicity in soil. During the agricultural planting process, once heavy metals in the soil enter the food chain through plants, they will pose great potential risks to food security and human health.

[0003] To address the problem of heavy metal pollution remediation, the heavy metal content can currently be reduced by leaching the soil with humic acid. Humic acid can passivate a variety of heavy metals in the soil, and at the same time can reduce the enrichment of heavy metals in plants and reduce the heavy metal content in plants and seeds. It is an effective method to reduce the heavy metal content.

[0004] However, the price of humic acid is relatively high. If purchased humic acid is used for soil leaching, the cost is very high. Therefore, there is an urgent need for a soil conditioner that can effectively improve heavy metals in the soil at a low cost.

[0005] Public Content

[0006] In order to solve the problems of the prior art, the present disclosure provides a white rot fungus WH-B-X2 used as a soil biomodifier and its application, a soil biomodifier and its preparation method. The technical solution is as follows:

[0007] On the one hand, the present disclosure provides a white rot fungus WH-B-X2 for use as a soil biomodifier, wherein the white rot fungus WH-B-X2 was deposited in the China Center for Type Culture Collection on May 8, 2024, with the deposit address being Wuhan University, Wuhan, China, and the deposit number being CCTCC NO:M 2024883.

[0008] On the other hand, the present disclosure provides a use of white rot fungus WH-B-X2, wherein the white rot fungus WH-B-X2 is used as a soil biomodifier.

[0009] On the other hand, the soil biological conditioner includes: the above-mentioned white rot fungus WH-B-X2 and compound fertilizer.

[0010] In another aspect, the present disclosure provides a method for preparing a soil biomodifier, characterized in that the method comprises:

[0011] Cultivating the white rot fungus WH-B-X2 as claimed in claim 1 in the first straw to obtain a primary solid fungus;

[0012] or inoculating the strain of the white rot fungus WH-B-X2 as claimed in claim 1 into a first liquid strain culture medium to obtain a primary liquid strain;

[0013] Adding the first-level solid bacterial strain or the first-level liquid bacterial strain into a solid bacterial strain fermentation vessel containing the second straw for culturing to obtain a second-level solid bacterial strain;

[0014] Adding the secondary solid bacterial strain into a large fermentation tank filled with a second liquid bacterial strain culture medium for cultivation to obtain a tertiary bacterial strain;

[0015] aerobically fermenting the third-level bacteria and the third straw in a fermentation vessel at room temperature to obtain a fermentation product;

[0016] The fermentation product is subjected to solid-liquid separation to obtain a solid product as the soil biomodifier.

[0017] Specifically, the first liquid culture medium and the second liquid culture medium have the same ingredients, and the first liquid culture medium includes: soybean meal powder, molasses, potassium dihydrogen phosphate, magnesium sulfate and water, and the weight portions of the soybean meal powder, the molasses, the potassium dihydrogen phosphate, the magnesium sulfate and the water are: 0.3-0.5 parts, 3-5 parts, 0.05-0.06 parts, 0.05-0.06 parts and 119.7-126 parts respectively.

[0018] Specifically, the method also includes pre-treatment of the first straw and the second straw, crushing the first straw and the second straw to 40-60 mesh, then removing impurities and spraying and soaking, so that the moisture content of the first straw and the second straw is 62-67%, and then sterilizing the first straw and the second straw.

[0019] Specifically, the solid strain fermentation vessel is the solid strain fermentation vessel provided by application number 202220918864.1.

[0020] Furthermore, the method also includes: adding the configured second liquid culture medium into a large fermentation tank and sterilizing it; then reversely pressing a portion of the second liquid culture medium into the solid culture fermentation vessel, stirring and mixing a portion of the second liquid culture medium with the secondary solid culture to obtain a mixture, and pressing the mixture in the solid culture fermentation vessel into the large fermentation tank for culturing to obtain the tertiary culture.

[0021] Furthermore, the temperature in the large fermentation tank is 28°C, the tank pressure is 0.05 MPa, the fan blades of the large fermentation tank are used for stirring during the culture process, and the rotation speed of the large fermentation tank is 100 rpm. The air valve of the large fermentation tank is opened for ventilation, and the ventilation volume is 0.7 m 3 / h. When the dissolved oxygen content decreases after 43h to 46h of cultivation, adjust the ventilation rate to 1m 3 / h~1.2m 3 / h.

[0022] Specifically, the room temperature aerobic fermentation is carried out in a fermentation vessel provided by application number 201810874868.2, and the room temperature aerobic fermentation is carried out in an open environment. During the room temperature aerobic fermentation process, the four sides and the top of the fermentation vessel are covered with non-woven fabrics, and a bleach solution is sprayed once a day in the open environment to disinfect miscellaneous bacteria, and the concentration of the bleach solution is 0.3% to 0.5%.

[0023] Furthermore, the time of the normal temperature aerobic fermentation is 18 to 20 days, the temperature of the open environment is 10 to 32° C., the humidity of the open environment is 60 to 90%, and the humidity in the fermentation vessel is 55 to 70%.

[0024] The beneficial effects brought about by the technical solution provided by the embodiment of the present disclosure are as follows: the embodiment of the present invention provides a white rot fungus WH-B-X2 for use as a soil biological conditioner, and the white rot fungus WH-B-X2 is used as a soil biological conditioner. The hyphae of the white rot fungus WH-B-X2 has the functions of adsorption, passivation and separation of heavy metals. At the same time, the hyphae can absorb heavy metal ions such as iron, copper and manganese in the soil into the body, and can quickly decompose lignin, further form humic acid, increase fertility, and heavy metal ions that cannot be absorbed, such as cadmium, chromium and lead, can be passivated by the enzymes secreted by the white rot fungus WH-B-X2 to form oxides insoluble in water, thereby reducing the situation where heavy metals in the soil flow into groundwater through infiltration and the like to pollute water sources. At the same time, during the fermentation process of straw inoculated with white rot fungus WH-B-X2 , 27% humic acid will be produced. Humic acid is a macromolecular porous substance. It can adsorb heavy metal ions in the soil into its own groups, thereby protecting the plant roots from absorbing too many heavy metal ions; humic acid can also passivate metal ions such as cadmium, chromium, mercury and lead, making it difficult for them to enter the crop body, reducing the absorption of heavy metals by crops, and is a recognized excellent soil biological improver for heavy metal pollution; the embodiment of the present invention provides a method for preparing a soil biological improver, which greatly shortens the seed production time, thereby improving the seed production efficiency, thereby reducing the seed production cost, and the fermentation process does not require turning the pile, thereby further reducing the probability of infection by miscellaneous bacteria and shortening the fermentation time; the preparation method will produce 27% humic acid in the product during the fermentation process of straw inoculation with white rot fungus WH-B-X2. The soil biological improver provided in this embodiment has a good inhibitory effect on effective cadmium in the soil, and can significantly reduce the cadmium content in crops. At the same time, the soil biological improver provided in this embodiment can increase the pH value of the soil, so that the pH value of the soil can be suitable for crop growth and development, and avoid excessive soil acidity. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solutions in the embodiments of the present disclosure, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present disclosure. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0026] Figure 1 This is a comparison chart of the effective cadmium content in soil I and soil II provided in Example 2 of the present disclosure. DETAILED DESCRIPTION

[0027] In order to make the objectives, technical solutions and advantages of the present disclosure more clear, the embodiments of the present disclosure will be further described in detail below with reference to the accompanying drawings.

[0028] Embodiment 1

[0029] The present disclosure provides a white rot fungus WH-B-X2 used as a soil biomodifier, and the white rot fungus WH-B-X2 Pleurotus ostreatus WH-B-X2 was deposited in the China Center for Type Culture Collection on May 8, 2024, with a deposit address of Wuhan, China, Wuhan University, and a deposit number of CCTCC NO: M2024883. The white rot fungus WH-B-X2 belongs to the genus of wood-decaying fungi, and a large number of wood-decaying fungi and grass-decaying fungi are collected in nature, and inoculated into straw for aerobic fermentation experiments respectively, from which white rot fungi with strong ability to decompose lignin and cellulose are selected. At the same time, the white rot fungi are purified, optimized and cultivated against infection by miscellaneous bacteria to obtain the white rot fungus WH-B-X2 provided in the embodiment of the present invention, which makes the white rot fungus WH-B-X2 provided in the embodiment of the present invention suitable for aerobic fermentation and has good anti-miscellaneous bacteria ability.

[0030] Embodiment 2

[0031] The embodiment of the present invention provides a method for preparing a soil bio-modifier, which uses a primary solid bacterial strain and specifically includes:

[0032] The straw is crushed, dusted and infiltrated to obtain a first straw, wherein the first straw has a moisture content of 62-67% after infiltration. The first straw is placed in a triangular flask with a magnetic rotor, and the triangular flask is sterilized in a steam sterilization furnace to obtain a first-level straw solid bacterial culture medium; the sterilization temperature is 121-123° C., the sterilization pressure is 0.1 MPa, and the sterilization time is 30-50 min. After sterilization, the temperature is reduced to 25-28° C. and can be used for subsequent operations;

[0033] The strain of white rot fungus WH-B-X2 cultured on the plate is inoculated into a triangular flask containing a first-level straw solid strain culture medium, with an inoculation ratio of 2-10%, and cultured in an incubator at a temperature of 25-30°C for 5-9 days to obtain a first-level solid strain;

[0034] In the clean bench, add appropriate amount of sterilized water to the triangular flask containing the magnetic rotor and the primary straw solid culture medium for dilution, stir on a magnetic stirrer for 20 to 40 seconds, and set aside.

[0035] Observe the mycelial growth of white rot fungus WH-B-X2 with naked eyes. Figure 1 As shown, the mycelium of the white rot fungus WH-B-X2 fully grows through the primary straw solid culture medium, and is pure white in color without contamination by other bacteria, and can be used for subsequent expanded culture.

[0036] The straw is crushed, dusted and infiltrated to obtain a second straw, which is crushed to 40-60 meshes to obtain a second straw. The second straw is loaded into a solid bacterial culture fermentation vessel, which is a solid bacterial culture fermentation vessel provided by application number CN202220918864.1, and water is added and stirred evenly. In this embodiment, the water content of the second straw after infiltration is 62-67%. The second straw is sterilized at a sterilization temperature of 121-123° C., a sterilization pressure of 0.1 MPa, and a sterilization time of 30-50 min. After sterilization, the temperature is reduced to 25-28° C. and used as a solid bacterial culture medium for standby use, which can be used for subsequent operations;

[0037] Inoculate the first-level solid bacteria into the solid bacteria fermentation vessel containing the second straw at an inoculation rate of 2% to 10%, set the air pressure to 0.3 to 0.5 MPa, and culture at a temperature of 23 to 28° C. for 3 to 5 days to obtain the second-level solid bacteria;

[0038] The second liquid culture medium is prepared according to 80% of the total volume of the large fermentation tank, and the prepared second liquid culture medium is placed in the large fermentation tank. The second liquid culture medium is sterilized by steam at high temperature, the sterilization temperature is set to 121-123° C., the sterilization time is set to 30-50 minutes, and after the sterilization is completed, the temperature is lowered to 25-28° C. to obtain a sterilized second liquid culture medium.

[0039] After sterilizing the delivery pipeline connecting the solid strain fermentation vessel and the large fermentation tank, according to the mass ratio of the secondary solid strain to the second liquid strain culture medium of 1: (10-15), a part of the second liquid strain culture medium is reversely pressed from the large fermentation tank into the solid strain fermentation vessel, the secondary solid strain is diluted, the machine is turned on and stirred evenly, and then pressed into the large fermentation tank, mixed with the second liquid strain culture medium and inoculated, and liquid strain production is carried out to obtain tertiary liquid strains.

[0040] During the culture process, the temperature of the large fermentation tank was set at 28°C and the tank pressure was set at 0.05Mpa. During the fermentation, the fan was used for stirring, and the speed of the fan was 100rpm. The air valve was opened for ventilation, and the ventilation volume was 0.7m 3 / h, after 43h~46h, the dissolved oxygen content decreases, and the ventilation volume is adjusted to 1m 3 / h~1.2m 3 / h. After continuous cultivation for 24 hours, the third-level strain, namely the strain of liquid white rot fungus WH-B-X2 for production, was obtained.

[0041] Specifically, the second liquid culture medium includes: soybean meal powder, molasses, potassium dihydrogen phosphate, magnesium sulfate and water, and the weight portions of soybean meal powder, molasses, potassium dihydrogen phosphate, magnesium sulfate and water in the second liquid culture medium are 0.4 parts, 4 parts, 0.05 parts, 0.05 parts and 126 parts respectively.

[0042] 100 mL of the strain of liquid white rot fungus WH-B-X2 for production was taken and placed in a conical flask, and its pH value was measured using a pH meter. The pH value of the strain of liquid white rot fungus WH-B-X2 for production provided in this embodiment was 6.0. When the pH value was 5.0-7.0, it indicated that the strain of liquid white rot fungus WH-B-X2 for production was in good growth state.

[0043] After the strain of liquid white-rot fungi WH-B-X2 for production in the conical flask was left to stand for 3 hours and observed, it was found that the bacterial liquid was clear and not turbid, and the bacterial balls grew evenly, accounting for more than 90% of the total volume, which indicated that the strain of liquid white-rot fungi WH-B-X2 for production met the requirements.

[0044] Then the strain of liquid white rot fungus WH-B-X2 for production was examined under a microscope, specifically including: using a pipette to absorb the above bacterial liquid, dropping a drop (including bacterial balls) onto a glass slide, and carefully observing its state under a microscope with a 40x objective lens. It can be seen that the liquid strain in the field of view grows plumply without foreign bacteria, which indicates that the culture process is good.

[0045] The straw is processed by a spray, crushing, infiltration and dust removal unit for agricultural organic waste provided by Patent No. ZL202221165864.5, so that the length of the crushed straw is 2 to 10 cm, and the third straw is obtained. The third straw is soaked for 3 hours to make the water content of the third straw 50 to 60%.

[0046] The third straw is subjected to steam high temperature sterilization, the temperature is set at 121-123°C, and the time is set at 15-18 minutes;

[0047] The sterilized third straw is sent to the automatic weighing and proportioning inoculation machine. During the conveying process, the temperature of the third straw is quickly cooled to 25℃~28℃ by the fan unit on the conveyor belt.

[0048] After sterilizing the conveying pipeline connecting the large fermentation tank of the third-level liquid strain and the automatic weighing and proportioning inoculator provided by the application number 202221028709.9, the third-level strain and the sterilized third straw are simultaneously sent into the inoculator for automatic weighing and proportioning inoculation. The inoculation amount is set to 15% to 25%, the stirring is 30s, and the inoculated third straw is automatically unloaded into the fermentation vessel with the application number 201810874868.2, and aerobic fermentation is carried out at room temperature in an open environment. The time for aerobic fermentation at room temperature is 18 to 20 days, the temperature of the open environment is 10 to 32°C, the humidity of the open environment is 60 to 90%, the humidity in the solid strain fermentation vessel is 55 to 70%, and the thickness of the third straw in the fermentation vessel can be 40 to 120 cm.

[0049] After the fermentation, it can be seen with the naked eye that the white rot fungus WH-B-X2 has grown all over the solid culture fermentation vessel, and the mycelium is white in color, which indicates that the fermentation effect is good.

[0050] The entire fermentation process is carried out in the fermentation vessel. Through the unique physical structure of the fermentation vessel, the heat generated by the fermentation of the third straw in the fermentation vessel causes the air to expand due to heat, which discharges the heat and drives cold air into the fermentation vessel. This cycle is repeated to ventilate, dissipate heat and provide oxygen for the normal temperature aerobic fermentation of white rot fungi. Avoiding heat dissipation and oxygen supply by turning over and tossing, thereby avoiding the fermentation process from being interrupted by humans, thereby shortening the fermentation time, ensuring the continuity of fermentation, and avoiding the problem of infection caused by miscellaneous bacteria due to heat dissipation caused by turning over and tossing. On the 8th day after the inoculation of the third straw, the hyphae of the white rot fungi can grow all over the fermentation material and cover the fermentation vessel, shortening the entire fermentation time to 18 to 20 days.

[0051] The fermentation product together with the fermentation vessel is transported to the box material unloading platform with patent number ZL 202021738846.2 for unloading, broken up and transported to the solid-liquid separator for solid-liquid separation. The rotation speed of the solid-liquid separator is 1460rpm; the solid fermentation product obtained after solid-liquid separation is the soil improver.

[0052] In this embodiment, a spraying, crushing, infiltration and dust removal unit for agricultural organic waste provided by Patent No. ZL202221165864.5 is used to pre-treat the third straw.

[0053] Specifically, the third straw is steam sterilized using the herbal crushed material high temperature steam sterilizer provided by application number 202021737630.4, the sterilization temperature is 121-123°C, and the sterilization time is 18-25 minutes.

[0054] Furthermore, the preparation method further comprises: cooling the sterilized third straw. In the present embodiment, the third straw can be cooled to 25-28° C. by blowing air through a series of fans during the transportation process.

[0055] Specifically, the inoculation amount of the first straw inoculated white rot fungus liquid strain is 10-25%.

[0056] In this embodiment, a high-temperature sterilized material automatic weighing, cooling and proportioning inoculation integrated machine provided by application number 202021028709.9 is used for automatic temperature calibration, weighing and proportioning inoculation, and the automatic temperature calibration temperature is set to within 30°C;

[0057] Specifically, the first straw, the second straw and the third straw can all be at least one of wheat straw, rice straw, corn straw, potato straw, rape straw, cotton straw and sugarcane straw. In this embodiment, the first straw, the second straw and the third straw are all wheat straw.

[0058] White rot fungi use straw as food during the fermentation process. While decomposing the straw, the fungus population reproduces rapidly and explosively. The effective live bacteria per gram is as high as 147 million (testing basis is GB 20287-2006), the mass fraction of organic matter is 71%, the total nutrients are 11.01%; on a dry basis, nitrogen is 1.68%, K 2 O is 1.49%, humic acid is 27%, and pH is 8.28.

[0059] Embodiment 3

[0060] This embodiment provides a use of white rot fungus WH-B-X2, which is used as a soil biomodifier. The white rot fungus in the soil biomodifier is 1.47×10 8 / gram.

[0061] The soil bio-modifier prepared in Example 2 of the present invention was used to carry out an improvement experiment, as follows:

[0062] The test soils were two samples of soil contaminated with heavy metals such as cadmium provided by the Xichuan County Agriculture and Rural Affairs Bureau, namely soil I and soil II. The basic physical and chemical properties of soil I and soil II are shown in Table 1.

[0063] Table 1 shows the basic physical and chemical properties of the two cadmium-contaminated soils

[0064] Soil No. Soil I (S1) Soil II (S2) pH 6.07 6.01 Organic matter (g / kg) 27.12 21.57 Total nitrogen (g / kg) 1.37 1.33 Available phosphorus (mg / kg) 19.72 17.89 Available potassium (mg / kg) 136.55 124.5 Total cadmium (mg / kg) 3.72 2.96 Weak acid extractable cadmium (mg / kg) 0.42 0.28 Reducible cadmium (mg / kg) 1.25 0.59 Oxidizable cadmium (mg / kg) 0.14 0.10 Residual cadmium (mg / kg) 1.91 1.99

[0065] The test crop is corn, specifically Denghai 605.

[0066] The test corn will be planted in soil I and soil II from November 2022 to May 2023, respectively.

[0067] Six groups were set for both soil I and soil II, including: a blank group (no material was applied); a control group 1 (2.9g of ternary compound fertilizer was applied to each pot); a control group 2 (2.9g of ternary compound fertilizer was applied to each pot + a commercial organic fertilizer with a concentration of 0.5%); a control group 3 (2.9g of ternary compound fertilizer was applied to each pot + a commercial organic fertilizer with a concentration of 1.0%); an experimental group 1 (2.9g of ternary compound fertilizer was applied to each pot + a soil biological improver prepared in Example 2 of the present invention with a concentration of 0.5%); an experimental group 2 (2.9g of ternary compound fertilizer was applied to each pot + a soil biological improver prepared in Example 2 of the present invention with a concentration of 1.0%). The ternary compound fertilizer used in this embodiment is a commercially available NPK compound fertilizer with a specification of 26-10-15; the commercial organic fertilizer is an organic fertilizer obtained by the manufacturer through high temperature anaerobic fermentation of animal feces.

[0068] All soils were sieved through a 5mm sieve to remove gravel, plant roots and other debris. Fertilizer was used as base fertilizer and mixed with the soil thoroughly. Each pot was filled with 7.5kg of soil and 5 corn plants were planted in each pot. Four replicates were set up for each group, and the experiments were arranged in completely randomized blocks. Watering was carried out according to the water requirement of corn during its growth period, and management was carried out according to the production methods of high-quality corn.

[0069] After 15 days, the height of corn plants was measured and recorded. The results are shown in Table 2.

[0070] Table 2 shows the height of corn plants.

[0071]

[0072] It can be seen from Table 2 that compared with the blank group, control group 1 and control group 2, experimental group 1 and experimental group 2 can effectively promote the growth of corn.

[0073] At the same time, the content of available cadmium in soil Ⅰ and soil Ⅱ was determined respectively. The available cadmium in soil was measured by CaCl 2 Extraction method (Zhang Xiuzhi et al. 2021), the results are as follows Figure 1 As shown. Figure 1 It can be seen that for soil I, the effective cadmium content of experimental group 1 and experimental group 2 was significantly reduced compared with the blank group, comparison group 1 and comparison group 2; for soil II, the effective cadmium content of experimental group 2 was reduced compared with the blank group, comparison group 1 and comparison group 2.

[0074] Different dosages have different effects on the effective cadmium in the soil. The increase from 0.5% in experimental group 1 to 1% in experimental group 1 increased the effective cadmium content in soil I and soil II from 21.60% to 31.57% and from 8.81% to 18.58%, respectively. The dosage of comparison group 2 increased from 0.5% to 1% in comparison group 3, which increased the effective cadmium content in soil I and soil II from 12.61% to 14.74% and from 7.67% to 12.37%, respectively. It can be seen that compared with increasing the dosage of commercial organic fertilizer, the soil biological conditioner provided in this embodiment has a better inhibitory effect on the effective cadmium in the soil.

[0075] The cadmium content in the root system and kernels of corn was measured respectively. Compared with the blank group, control group 1 and control group 2, the cadmium content in the root system of corn in experimental group 1 and experimental group 2 decreased by 34.41%, and the cadmium content in the kernels of corn in experimental group 1 and experimental group 2 decreased by 31.59%. It can be seen that experimental group 1 and experimental group 2 can significantly reduce the cadmium content in corn.

[0076] The pH values ​​of soil I and soil II were measured respectively. Compared with the blank group, the pH values ​​of soil I in comparison group 2 and comparison group 3 decreased by 0.41-0.49, and compared with the blank group, the pH values ​​of soil II in comparison group 2 and comparison group 3 decreased by 0.32-0.61. This may be because the commercial organic fertilizer is decomposed by straw, has a high C / N value, decomposes slowly in the soil, and can release more organic acids, causing the pH value of the soil to decrease. Too low a pH value will make the soil too acidic, thereby slowing the growth and development of crops. The soil biological conditioner provided in this embodiment can increase the pH value of the soil, so that the pH value of the soil can be suitable for crop growth and development, and avoid excessive soil acidity.

[0077] The above description is only an optional embodiment of the present disclosure and is not intended to limit the present disclosure. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present disclosure shall be included in the protection scope of the present disclosure.

Claims

1. A white rot fungus WH-B-X2 for use as a soil conditioner, characterized in that: The white rot fungus WH-B-X2 was deposited in the China Center for Type Culture Collection on May 8, 2024, with the deposit address being Wuhan University, Wuhan, China, and the deposit number being CCTCC NO:M 2024883.

2. An application of white rot fungus WH-B-X2, characterized in that: The application includes: using the white rot fungus WH-B-X2 as a soil biological conditioner.

3. A soil biological conditioner, characterized in that: The soil biological conditioner comprises: the white rot fungus WH-B-X2 as claimed in claim 1 and compound fertilizer.

4. A method for preparing a soil biological conditioner, characterized in that: The method comprises: Cultivating the white rot fungus WH-B-X2 as claimed in claim 1 in the first straw to obtain a primary solid fungus; or inoculating the strain of the white rot fungus WH-B-X2 as claimed in claim 1 into a first liquid strain culture medium to obtain a primary liquid strain; Adding the first-level solid bacterial strain or the first-level liquid bacterial strain into a solid bacterial strain fermentation vessel containing the second straw for culturing to obtain a second-level solid bacterial strain; Adding the secondary solid bacterial strain into a large fermentation tank filled with a second liquid bacterial strain culture medium for cultivation to obtain a tertiary bacterial strain; aerobically fermenting the third-level bacteria and the third straw in a fermentation vessel at room temperature to obtain a fermentation product; The fermentation product is subjected to solid-liquid separation to obtain a solid product as the soil biomodifier.

5. The method for preparing the soil biological improver according to claim 4, characterized in that: The first liquid culture medium and the second liquid culture medium have the same ingredients, and the first liquid culture medium includes: soybean meal powder, molasses, potassium dihydrogen phosphate, magnesium sulfate and water, and the weight portions of the soybean meal powder, the molasses, the potassium dihydrogen phosphate, the magnesium sulfate and the water are: 0.3-0.5 parts, 3-5 parts, 0.05-0.06 parts, 0.05-0.06 parts and 119.7-126 parts respectively.

6. The method for preparing the soil biological conditioner according to claim 4, characterized in that: The method further comprises pre-treatment of the first straw and the second straw, crushing the first straw and the second straw to 40-60 mesh, removing impurities and spraying and soaking, so that the moisture content of the first straw and the second straw is 62-67%, and then sterilizing the first straw and the second straw.

7. The method for preparing the soil biological improver according to claim 4, characterized in that: The solid strain fermentation vessel is the solid strain fermentation vessel provided by application number 202220918864.

1.

8. The method for preparing the soil biological improver according to claim 7, characterized in that: The method also includes: adding the configured second liquid culture medium into a large fermentation tank and sterilizing it; then reversely pressing a portion of the second liquid culture medium into the solid culture fermentation vessel, stirring and mixing a portion of the second liquid culture medium with the secondary solid culture to obtain a mixture, and pressing the mixture in the solid culture fermentation vessel into the large fermentation tank for culturing to obtain the tertiary culture.

9. The method for preparing the soil biological improver according to claim 4, characterized in that: The room-temperature aerobic fermentation is carried out in a fermentation vessel provided by application number 201810874868.2, and is carried out in an open environment. During the room-temperature aerobic fermentation process, the four sides and the top of the fermentation vessel are covered with non-woven fabrics, and a bleaching solution is sprayed once a day in the open environment to disinfect miscellaneous bacteria, and the concentration of the bleaching solution is 0.3% to 0.5%.

10. The method for preparing the soil biological improver according to claim 9, characterized in that: The time of the normal temperature aerobic fermentation is 18 to 20 days, the temperature of the open environment is 10 to 32° C., the humidity of the open environment is 60 to 90%, and the humidity in the fermentation vessel is 55 to 70%.

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