Intelligent Ultra-low Temperature Biological Sample Storage Cabinet and Usage Method

Abstract

The present invention discloses an intelligent ultra-low temperature biological sample storage cabinet and a method of use, comprising a cabinet body and sample tubes, a storage assembly installed inside the cabinet body, a placement and removal assembly installed inside the cabinet body, the placement and removal assembly being used to move the sample tubes into the interior of the storage assembly, the storage assembly comprising a rotation placement assembly and a drive assembly for driving the rotation placement assembly to rotate; through the carefully designed storage assembly and placement and removal assembly, sample tubes loaded with biological samples can be easily and safely stored inside the cabinet body, or accurately removed from the depths of the cabinet body, not only realizing intelligent storage and access of sample tubes, but also greatly improving the work efficiency of experimenters when processing a large number of samples, and effectively reducing the risk of sample damage by reducing manual intervention and outdoor operations. At the same time, it also achieves the maximization of sample storage capacity, providing a more convenient and efficient sample management solution for biological experiments.

Description

Technical Field

[0001] The present invention relates to the technical field of biological sample storage, and in particular to an intelligent ultra-low temperature biological sample storage cabinet and a method of use. Background Art

[0002] Various biological samples such as tissues, blood, cells, organs, DNA, RNA, biological fluids, etc. generally need to be stored at low temperatures. Most of them are stored at -80°C, and the storage containers are ultra-low temperature manual refrigerators.

[0003] In traditional ultra-low temperature manual refrigerators, storing or removing biological samples is done manually. There is a high probability of error during manual operation, and there is a risk of frostbite. At the same time, since the refrigerator door must be opened every time to store and retrieve biological samples, this process will bring in a large amount of water vapor, causing severe frost on the sample surface, affecting sample identification.

[0004] As biological sample storage has gained increasing attention, automated storage equipment has been introduced and gradually replaced ultra-low temperature manual refrigerators. However, existing fully automated ultra-low temperature biological sample storage equipment, such as CN110589332A, an automated biological sample library, although equipped with a turntable rotation module, a basket lifting module, a shovel module, a tube picking module, and a transfer module, has a relatively complex structure. The complex internal structure has led to a significant increase in the manufacturing cost of the equipment, which has also increased the difficulty and cost of maintenance to a certain extent. In addition, existing equipment generally adopts a compartmentalized storage design, which not only reduces the actual available storage space but also affects the storage efficiency of samples. In particular, when the demand for samples is large or a large number of samples need to be stored for a long time, the low effective volume ratio is more prominent, which greatly limits the application scope and applicability of the equipment. Summary of the Invention

[0005] The purpose of the present invention is to provide an intelligent ultra-low temperature biological sample storage cabinet and a method of use to solve the problems raised in the above background technology.

[0006] To achieve the above-mentioned object, the present invention provides the following technical solution: an intelligent ultra-low temperature biological sample storage cabinet, comprising a cabinet body and sample tubes, a storage assembly installed inside the cabinet body, and a placement and removal assembly installed inside the cabinet body, wherein the placement and removal assembly is used to move the sample tubes into the interior of the storage assembly;

[0007] The storage assembly includes a rotating placement assembly and a driving assembly for driving the rotating placement assembly to rotate. The rotating placement assembly includes a rotating frame. A plurality of entry and exit slots are provided inside the rotating frame wall. A plurality of storage frames are installed on the inner side of the rotating frame, and the positions of the plurality of storage frames correspond to the positions of the entry and exit slots respectively. An ejection port is provided on one side of each storage frame.

[0008] Preferably, the driving assembly includes a support base, a driving motor is fixedly installed inside the support base, the output end of the driving motor is fixedly connected to the bottom of the rotating frame, a plurality of entry and exit slots are provided inside the rotating frame, a partition is fixedly installed inside the cabinet, the support base is fixedly installed on the top of the partition, circular through holes are provided on the top and both sides of the storage frame, and a plurality of rectangular slot groups are provided inside the rotating frame.

[0009] Preferably, the rectangular slot groups are arranged in a plurality of groups at intervals along the circumferential direction of the side wall of the rotating frame, each rectangular slot group includes a plurality of strip-shaped heat dissipation holes longitudinally arranged along the height direction of the rotating frame, and a plurality of storage frames are arranged from top to bottom on the inner wall of the rotating frame between any two rectangular slot groups, each of the storage frames is in a cube shape, and the storage frames are arranged corresponding to the inlet and outlet slots, and the driving motor drives the rotating frame to rotate, and the driving motor is provided with a first magnetic induction switch, and a magnetic induction sheet is provided in the middle of the lower outer side wall of each storage frame located around the bottom of the rotating frame, and each of the magnetic induction sheets forms a unique positional correspondence with the plurality of storage frames above the same column;

[0010] A control box is fixedly installed at the bottom of the inner cavity of the cabinet, and a controller is installed in the control box. The controller is electrically connected to the drive motor and the first magnetic induction switch respectively. During the access process of the sample tube, after determining the target storage frame corresponding to the access of the sample tube, the corresponding magnetic induction piece is locked according to the position of the target storage frame, and the controller controls the drive motor to drive the rotating frame to rotate. At the same time, during the rotation process, the first magnetic induction switch senses the signal of each magnetic induction piece in real time. When the first magnetic induction switch senses the corresponding magnetic induction piece, the controller determines that the target storage frame has been reached and controls the drive motor to stop working.

[0011] Preferably, a switch button is installed on the front of the cabinet, a control display screen is installed on the front of the cabinet, an entry and exit frame is installed on the front of the cabinet, an entry and exit slots are provided on the top of the entry and exit frame, an intelligent sealing door is installed on the top of the entry and exit frame, a sealing slide groove is provided on one side of the entry and exit frame, a sealing slider is slidably installed inside the sealing slide groove, a connecting block is fixedly installed on one side of the sealing slider, a sample plate is fixedly installed on the top of the connecting block, a movable seat is movably installed on the top of the sample plate, a placement seat is snap-fitted on the top of the movable seat, a sample tube is placed on the top of the placement seat, a control box is fixedly installed on the bottom of the cabinet inner cavity, and a programmed cooling instrument is fixedly installed on both sides of the cabinet inner cavity.

[0012] Preferably, the placement and removal component includes an adjusting electric cylinder, a connecting plate is fixedly installed on the output end of the adjusting electric cylinder, a limiting slide is fixedly installed on the bottom of one end of the connecting plate, a moving frame is fixedly installed on one end of the limiting slide, a moving block is slidably installed inside the moving frame, two springs are fixedly connected to the top of the moving block, and the top of the spring is fixedly connected to the bottom of the inner cavity of the moving frame, a pushing electric cylinder is fixedly installed on one side of the moving block, and a pushing plate is fixedly installed on the output end of the pushing electric cylinder.

[0013] Preferably, a connecting rod 2 is fixedly installed on the top of the connecting plate away from one end of the limiting slide plate, a connecting bending rod is fixedly installed on the top of the connecting rod 2, a connecting rod 1 is fixedly installed on the bottom of the connecting bending rod away from one end of the connecting rod 2, a moving frame 2 is fixedly installed on the bottom of the connecting rod 1, a moving block 2 is slidably installed inside the moving frame 2, two springs 2 are fixedly connected to the top of the moving block 2, and the top of the spring 2 is fixedly connected to the bottom of the inner cavity of the moving frame 2, a pushing electric cylinder 2 is fixedly installed on one side of the moving block 2, and a pushing plate 2 is fixedly installed on the output end of the pushing electric cylinder 2, and the moving frame 2 and the pushing electric cylinder 2 are both located on the inner side of the rotating frame.

[0014] Preferably, a fixed connecting rod is fixedly installed on the bottom of the connecting plate away from the limiting slide, and one side of the bottom of the fixed connecting rod is fixedly connected to the end of the sealing slider away from the connecting block. A vertical plate is fixedly installed inside the cabinet, and a limiting slide groove is provided inside the vertical plate. The limiting slide is slidably installed on the inner side of the limiting slide groove, and an elastic sealing belt is installed inside the sealing slide groove, and the elastic sealing belt is fixedly connected to the bottom of the sealing slider.

[0015] Preferably, an inverted T-shaped slide groove 1 is provided inside the sample plate, an inverted T-shaped slider is fixedly installed on the bottom of the movable seat, and the inverted T-shaped slider is slidably installed on the inner side of the sample plate. An inverted T-shaped slide groove 2 is provided inside the rotating frame and the storage frame, and the specifications and dimensions of the inverted T-shaped slide groove 2 are compatible with the specifications and dimensions of the inverted T-shaped slider.

[0016] Preferably, an outer protective shell is fixedly installed on the outside of the cabinet, and observation windows are installed inside the outer protective shell and the cabinet. Inspection doors are installed on both sides of the cabinet, and heat dissipation ports are opened on both sides of the cabinet. Programmed cooling devices are installed on both sides of the inner cavity of the cabinet, and the programmed cooling devices cool down the inside of the cabinet. The heat dissipation ports correspond to the heat dissipation points of the programmed cooling devices. An RFID scanner is installed on the front of the cabinet, and a USB interface is installed on the front of the cabinet.

[0017] Preferably, the rotating frame wall is equipped with a plurality of temperature sensors, and the controller is electrically connected to the program cooling device and the temperature sensors respectively, and the controller is used to control the temperature of the program cooling device, including:

[0018] Get the program cooling device start control instruction;

[0019] The program cooling device is started according to a program cooling device start control instruction, and a response time of the program cooling device start control instruction is obtained;

[0020] When the program cooling instrument cools down the sample tube inside the cabinet, the temperature change information of the temperature sensor is obtained in real time;

[0021] Analyze the temperature change information of the temperature sensor to determine the temperature change characteristics inside the cabinet;

[0022] Based on the temperature change characteristics inside the cabinet, the cooling temperature is predicted to obtain the cooling prediction information inside the cabinet;

[0023] The target analysis time is determined according to the response time of the program cooling instrument start control instruction. The cabinet temperature drop prediction information is analyzed in combination with the target analysis time to determine whether the cabinet internal temperature reaches the minimum cabinet internal temperature after the target analysis time, and obtain the prediction analysis result;

[0024] When the predicted analysis result shows that the temperature inside the cabinet does not reach the minimum temperature inside the cabinet after the target analysis time, the programmed cooling device continues to cool the inside of the cabinet;

[0025] When the predicted analysis result shows that the temperature inside the cabinet reaches the minimum value of the temperature inside the cabinet after the target analysis time, a program cooling device pause control instruction is obtained to stop the program cooling device from cooling the inside of the cabinet (1).

[0026] A method for using an intelligent ultra-low temperature biological sample storage cabinet comprises the following steps:

[0027] S1: Input information: Before placing the sample tube for storage, the RFID tag on the outside of the sample tube can be scanned by an RFID scanner. The scan result will be transmitted to the control box. The processor inside the control box can process the sample information of the sample tube and start the program cooling device to cool the inside of the cabinet so that the temperature inside the cabinet is suitable for storing the sample inside the sample tube;

[0028] S2: Sample storage: First, place the sample tube on top of the placement seat. Then, input instructions through the control display screen to activate the adjustment electric cylinder inside the placement and removal component, and then the output end retracts, so that the sample tube enters the interior of the cabinet. In addition, after the drive motor is activated, it can drive the rotating frame to rotate. By adjusting the coordination of the electric cylinder and the drive motor, the sample plate can be aligned with the position of one of the entry and exit slots and the storage frame. Then, the push electric cylinder 1 is activated to embed the movable seat carrying the placement seat and the sample tube into the storage frame, thereby completing the storage of the sample tube.

[0029] S3: Sample removal: When the output end of the control adjustment electric cylinder drives the sample plate to move to the front of the storage frame where the sample tube that needs to be removed is stored, the pushing electric cylinder 2 is started. The output end of the pushing electric cylinder 2 can push the pushing plate 2 to move, thereby pushing the moving seat carrying the placement seat and the sample tube to the top of the sample plate, and then the output end of the adjusting electric cylinder is started to extend, thereby removing the sample tube from the inside of the cabinet.

[0030] Compared with existing technologies, the present invention offers the following advantages: through carefully designed storage and placement / retrieval components, sample tubes containing biological samples can be easily and safely stored inside the cabinet or accurately retrieved from deep within the cabinet. This not only enables intelligent storage and retrieval of sample tubes, but also greatly improves the efficiency of laboratory personnel when handling large numbers of samples. Furthermore, by reducing manual intervention and outdoor operations, the risk of sample damage is effectively reduced. Furthermore, it maximizes sample storage capacity, providing a more convenient and efficient sample management solution for biological experiments.

[0031] The storage components ensure that the sample tubes are stored securely within the cabinet and facilitate quick positioning, ensuring that the sample tubes do not interfere with each other during storage and are not damaged by vibration or temperature changes. This design not only improves storage density but also optimizes space utilization, allowing the cabinet to accommodate more sample tubes. The rotating frame can rotate around the center point, allowing different storage frames to be aligned with the access ports of the sample plate in sequence, which not only increases the flexibility of sample storage but also allows experimenters to quickly select and access specific sample tubes as needed. The provision of multiple storage frames further increases the sample storage capacity, meeting the high demand for sample storage in large-scale biological experiments.

[0032] The placement and removal component uses a highly automated mechanical structure to achieve fast and accurate placement and removal of sample tubes. Experimenters only need to use simple operating instructions to allow the placement and removal component to automatically complete the transportation of sample tubes. During this process, there is no need to manually open the cabinet door directly, thus avoiding the risk of contamination caused by outdoor operations and ensuring the purity and integrity of the samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 It is a schematic diagram of the three-dimensional appearance structure of the present invention.

[0034] Figure 2 For the present invention Figure 1 Enlarged structural diagram at point A in the middle.

[0035] Figure 3 It is a schematic diagram of the cross-sectional structure of the cabinet of the present invention.

[0036] Figure 4 It is a schematic diagram of the three-dimensional structure of the storage component and the placement and removal component of the present invention.

[0037] Figure 5 It is a schematic diagram of the three-dimensional structure of the rotating placement component of the present invention.

[0038] Figure 6 This is a schematic diagram of the three-dimensional structure of the placement and removal component of the present invention.

[0039] Figure 7 This is a schematic diagram of the three-dimensional structure of the placement and removal component from another perspective of the present invention.

[0040] Figure 8 A flow chart diagram is used for the present invention.

[0041] Figure: 1, cabinet; 2, outer protective shell; 3, observation window; 4, switch button; 5, RFID scanner; 6, USB interface; 7, control display; 8, access frame; 9, maintenance door; 10, heat dissipation vent; 11, sample tube; 12, placement seat; 13, moving seat; 14, sample plate; 15, inverted T-shaped slide; 16, inverted T-shaped slider; 17, connecting block; 18, sealing slide; 19, rotating frame; 20, partition; 21, support seat; 22, control box; 23, program cooling device; 24, connecting bending rod; 25, rectangular slot group; 26, storage frame; 2 7. Circular through hole; 28. Entry and exit slots; 29. Intelligent sealing door; 30. Moving frame 1; 31. Vertical plate; 32. Limiting slide; 33. Inverted T-shaped slide 2; 34. Connecting rod 1; 35. Push-out port; 36. Adjusting electric cylinder; 37. Connecting rod 2; 38. Driving motor; 39. Spring 2; 40. Moving frame 2; 41. Moving block 2; 42. Pushing electric cylinder 2; 43. Pushing plate 2; 44. Connecting plate; 45. Limiting slide; 46. Sealing slider; 47. Fixed connecting rod; 48. Spring 1; 49. Moving block 1; 50. Pushing electric cylinder 1; 51. Pushing plate 1. DETAILED DESCRIPTION

[0042] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0043] See also Figures 1-8 The present invention provides a technical solution: an intelligent ultra-low temperature biological sample storage cabinet, comprising a cabinet body 1 and a sample tube 11, a storage component installed inside the cabinet body 1, the storage component including a support base 21, a drive motor 38 fixedly installed inside the support base 21, a rotating frame 19 fixedly installed at the output end of the drive motor 38, a plurality of inlet and outlet slots 28 are provided inside the rotating frame 19, a plurality of storage frames 26 are fixedly installed inside the rotating frame 19, and a push-out port 35 is provided on one side of each storage frame 26, a partition 20 is fixedly installed inside the cabinet body 1, the support base 21 is fixedly installed on the top of the partition 20, a circular through hole 27 is provided on the top and both sides of the storage frame 26, a plurality of rectangular slot groups 25 are provided inside the rotating frame 19, and the positions of the plurality of storage frames 26 correspond to the positions of the plurality of inlet and outlet slots 28 respectively.

[0044] See also Figure 4 and Figure 5 During the access process of the sample tubes 11, in order to accurately locate the corresponding storage frame 26 for access, the present invention provides a positioning assembly, which includes a magnetic sensor, a first magnetic induction switch, a controller, and a drive motor 38. Specifically, multiple groups of rectangular slots 25 are arranged along the circumference of the side wall of the rotating frame 19. Each group of rectangular slots 25 includes a plurality of strip-shaped heat dissipation holes arranged longitudinally along the height direction of the rotating frame 19. Multiple storage frames 26 are arranged from top to bottom on the inner wall of the rotating frame 19 between any two rectangular slot groups 25. Each storage frame 26 is in a cube shape and is arranged corresponding to the inlet and outlet slots 28. This structural arrangement can improve heat dissipation, ensuring that both sides of each storage frame 26 have heat dissipation strip-shaped heat dissipation holes, ensuring that the temperature inside the storage frame 26 is consistent with the internal temperature of the cabinet 1 to the greatest extent possible, avoiding local heat accumulation, thereby ensuring that the sample tubes 11 are stored at a low temperature and guaranteeing the storage conditions of the samples in the sample tubes 11.

[0045] During the sample storage and retrieval process, the drive motor 38 drives the rotating frame 19 to rotate. The drive motor 38 is equipped with a first magnetic induction switch. A magnetic induction plate is provided in the middle of the lower outer wall of each storage frame 26 located around the bottom of the rotating frame 19. Each magnetic induction plate forms a unique positional correspondence with the multiple storage frames 26 above the same column.

[0046] A control box 22 is fixedly installed at the bottom of the inner cavity of the cabinet 1, and a controller is installed in the control box 22. The controller is electrically connected to the drive motor 38 and the first magnetic induction switch respectively. During the access process of the sample tube 11, after determining the target storage frame 26 corresponding to the access of the sample tube 11, the corresponding magnetic induction piece is locked according to the position of the target storage frame 26. The controller controls the drive motor 38 to drive the rotating frame 19 to rotate. At the same time, during the rotation process, the first magnetic induction switch senses the signals of each magnetic induction piece in real time. When the first magnetic induction switch senses the corresponding magnetic induction piece, the controller determines that it has reached the target storage frame 26 and controls the drive motor 38 to stop working.

[0047] The principle and effect of the above technical solution are as follows: by forming a corresponding matching relationship between each group of storage frames 26 and a magnetic sensing piece, during the process of accessing the sample tube 11, it is necessary to determine the storage frame 26 corresponding to the access. If the storage frames 26 are in the same column, they can correspond to the same access position relationship, that is, the rotating frame 19 is rotated to the same position by driving the motor 38. During the rotation process, the signals of each magnetic sensing piece are sensed in real time by the first magnetic sensing switch, and each magnetic sensing piece is assigned a different label. Then, a corresponding relationship is formed between the magnetic sensing piece and the storage frame 26 in each column, and stored in the controller. In this way, when the first magnetic sensing switch senses the corresponding magnetic sensing piece, the controller can determine that the corresponding storage frame 26 has been moved to the required position, that is, the sample tube 11 can be accessed. The setting of this solution can ensure that during the access process of the sample tube 11, the required storage frame 26 can be quickly and intelligently rotated to the target position, thereby improving work efficiency and intelligence.

[0048] A placement and removal component is installed inside the cabinet 1, a switch button 4 is installed on the front of the cabinet 1, a control display screen 7 is installed on the front of the cabinet 1, an entry and exit frame 8 is installed on the front of the cabinet 1, an entry and exit slots are provided on the top of the entry and exit frame 8, an intelligent sealing door 29 is installed on the top of the entry and exit frame 8, a sealing slide 18 is provided on one side of the entry and exit frame 8, a sealing slider 46 is slidably installed inside the sealing slide 18, a connecting block 17 is fixedly installed on one side of the sealing slider 46, a sample plate 14 is fixedly installed on the top of the connecting block 17, a movable seat 13 is movably installed on the top of the sample plate 14, a placement seat 12 is snap-fitted on the top of the movable seat 13, and a sample tube 11 is placed on the top of the placement seat 12.

[0049] The placement and removal component includes an adjusting electric cylinder 36, the output end of the adjusting electric cylinder 36 is fixedly installed with a connecting plate 44, the bottom of one end of the connecting plate 44 is fixedly installed with a limiting slide 45, one end of the limiting slide 45 is fixedly installed with a moving frame 30, the interior of the moving frame 30 is slidingly installed with a moving block 49, the top of the moving block 49 is fixedly connected to two springs 48, and the top of the spring 48 is fixedly connected to the bottom of the inner cavity of the moving frame 30, one side of the moving block 49 is fixedly installed with a pushing electric cylinder 50, the output end of the pushing electric cylinder 50 is fixedly installed with a pushing plate 51, the top of the connecting plate 44 away from the limiting slide 45 is fixedly installed with a connecting rod 23, the top of the connecting rod 237 is fixedly installed with a connecting bending rod 24, the bottom of the connecting bending rod 24 away from the end of the connecting rod 237 is fixedly installed with a connecting rod 34, and the bottom of the connecting rod 34 is fixedly installed with a moving frame 240 The interior of the moving frame 40 is slidably installed with a moving block 2 41, and the top of the moving block 2 41 is fixedly connected to two springs 2 39, and the top of the spring 2 39 is fixedly connected to the bottom of the inner cavity of the moving frame 2 40, and a pushing electric cylinder 2 42 is fixedly installed on one side of the moving block 41, and a pushing plate 2 43 is fixedly installed on the output end of the pushing electric cylinder 2 42. The moving frame 2 40 and the pushing electric cylinder 2 42 are both located on the inner side of the rotating frame 19, and a fixed connecting rod 47 is fixedly installed on the bottom of the connecting plate 44 away from the end of the limiting slide 45. One side of the bottom of the fixed connecting rod 47 is fixedly connected to the end of the sealing slider 46 away from the connecting block 17. The interior of the cabinet 1 is fixedly installed with a vertical plate 31, and a limiting slide groove 32 is provided inside the vertical plate 31. The limiting slide 45 is slidably installed on the inner side of the limiting slide groove 32, and an elastic sealing belt is installed inside the sealing slide groove 18, and the elastic sealing belt is fixedly connected to the bottom of the sealing slider 46.

[0050] A program cooling device 23 is fixedly installed on both sides of the inner cavity of the cabinet 1. The program cooling device 23 cools the inside of the cabinet 1. Several temperature sensors are installed on the wall of the rotating frame 19. The temperature inside the cabinet 1 can be sensed by the temperature sensors. The controller is electrically connected to the program cooling device 23 and the temperature sensors respectively. The controller is used to control the temperature of the program cooling device 23, including:

[0051] Obtaining a start control instruction of the program cooling device 23;

[0052] The program cooling device 23 is started according to the program cooling device 23 start control instruction, and the response time of the program cooling device 23 start control instruction is obtained;

[0053] When the program cooling device 23 cools the sample tube 11 inside the cabinet 1, the temperature change information of the temperature sensor is obtained in real time;

[0054] Analyze the temperature change information of the temperature sensor to determine the temperature change characteristics inside the cabinet 1;

[0055] Based on the temperature change characteristics inside the cabinet 1, a cooling temperature prediction is performed to obtain the cooling prediction information inside the cabinet 1;

[0056] The target analysis time is determined according to the response time of the control instruction of the program cooling device 23, and the temperature drop prediction information in the cabinet 1 is analyzed in combination with the target analysis time to determine whether the internal temperature of the cabinet 1 reaches the minimum internal temperature of the cabinet 1 after the target analysis time, thereby obtaining the prediction analysis result;

[0057] When the predicted analysis result shows that the temperature inside the cabinet 1 does not reach the minimum temperature inside the cabinet 1 after the target analysis time, the programmed cooling device 23 continues to cool the inside of the cabinet 1;

[0058] When the predicted analysis result shows that the internal temperature of the cabinet 1 reaches the minimum internal temperature of the cabinet 1 after the target analysis time, a pause control instruction of the program cooling device 23 is obtained to stop the program cooling device 23 from cooling the interior of the cabinet 1 .

[0059] The minimum temperature value inside the cabinet 1 is determined based on the temperature adaptation range of the refrigerated storage of the samples in the cabinet 1 .

[0060] The technical effects of the above are as follows: intelligent temperature control of the cabinet 1 is achieved through the controller and the temperature sensor, so that the programmed cooling device 23 can better cool the storage environment of the sample tubes 11 in the cabinet 1, while also preventing the programmed cooling device 23 from excessively cooling the temperature inside the cabinet 1, thereby affecting the refrigerated storage of the sample tubes 11. Furthermore, during the intelligent temperature control process, by obtaining the response time of the programmed cooling device 23 to start the control instruction, the time delay in responding to the control instruction is clearly determined. Therefore, when the programmed cooling device 23 is stopped to cool the environment inside the cabinet 1, the time delay of the control instruction is fully considered, and the response to the programmed cooling device 23 pausing the control instruction, which causes the programmed cooling device 23 to cool the environment inside the cabinet 1, is avoided. This improves the accuracy of the programmed cooling device 23 in cooling the environment inside the cabinet 1, thereby improving the intelligent performance of the temperature environment control inside the cabinet 1 and providing a guarantee for the effective storage of the sample tubes 11.

[0061] The working principle of the above technical solution is as follows: after the external power supply is connected, the device is turned on by the switch button 4, and the program cooling device 23 is turned on to cool the inside of the cabinet 1, so that the temperature inside the cabinet 1 is suitable for preserving the sample inside the sample tube 11. When the sample tube 11 needs to be stored, the sample tube 11 can be placed on the top of the placement seat 12, and then the placement seat 12 is placed inside the movable seat 13, and then the movable seat 13 is placed on the top of the sample plate 14. Then, the command is input through the control display screen 7, and the command will be transmitted to the processor inside the control box 22, and then the processor will transmit the data to the controller, and the controller controls the adjustment cylinder 36 and the drive motor 38 to start. After the adjustment cylinder 36 is started, the output end retracts, thereby bringing The movable connecting plate 44 moves upward, and the sealing slider 46 is pulled upward inside the sealing slide 18 by the fixed connecting rod 47. When the sample plate 14 approaches the intelligent sealing door 29, the intelligent sealing door 29 will automatically open, so that the sample plate 14 carrying the movable seat 13, the placement seat 12 and the sample tube 11 enter the interior of the cabinet 1. After entering, the intelligent sealing door 29 will automatically close to ensure the sealing and safety of the interior of the cabinet 1. In addition, when the connecting plate 44 moves upward, it can drive the movable frame 30 to move upward through the connecting plate 44. When the movable frame 30 moves upward, the movable block 49 and the pushing electric cylinder 50 will first be in a stationary state under the action of the elastic force of the spring 48, and then the movable frame 30 will pull the movable block 1 through the spring 48. 49 and the push electric cylinder 50 move upward, and make the position of the push plate 51 correspond to the height of the position of the moving seat 13. In addition, after the drive motor 38 is started, it can drive the rotating frame 19 to rotate, so that one column of the storage frames 26 corresponds to the position of the sample plate 14. By adjusting the cooperation of the electric cylinder 36 and the drive motor 38, the sample plate 14 can correspond to the position of one of the inlet and outlet slots 28 and the storage frame 26. Then, the push electric cylinder 50 is started, and the output end of the push electric cylinder 50 can push the push plate 51 to move, so that the moving seat 13 is pushed away from the top of the sample plate 14 by the push plate 51, and the moving seat 13 carrying the placement seat 12 and the sample tube 11 is pushed into the interior of the storage frame 26, thereby completing the sample The storage of the tube 11, in addition, when sampling is needed, when the output end of the adjusting electric cylinder 36 drives the sample plate 14 to move to the front of the storage frame 26 where the movable seat 13, the placement seat 12 and the sample tube 11 that need to be taken out are stored, the connecting plate 44 can also drive the movable frame 2 40 to move through the connecting rod 2 37, the connecting bending rod 24 and the connecting rod 1 34, so that the pushing electric cylinder 2 42 and the pushing plate 2 43 are aligned with the ejection port 35 on the back of the storage frame 26, and then the pushing electric cylinder 2 42 is started. The output end of the pushing electric cylinder 2 42 can push the pushing plate 2 43 to move, so that the movable seat 13 carrying the placement seat 12 and the sample tube 11 are pushed to the top of the sample plate 14 through the pushing plate 2 43, and then the output end of the adjusting electric cylinder 36 is started to extend.The sample plate 14, carrying the movable seat 13, the placement seat 12, and the sample tube 11, is then brought close to the intelligent sealing door 29. The intelligent sealing door 29 automatically opens, allowing the sample plate 14, carrying the movable seat 13, the placement seat 12, and the sample tube 11 to be removed from the interior of the cabinet 1, making it easier for the experimenter to perform sampling operations and enabling intelligent access to the sample tube 11. This greatly improves the efficiency of the experimenter's access to the sample tube 11, and eliminates the need to manually open the cabinet door, avoiding outdoor operations and sample damage. Furthermore, the rotation of the rotating frame 19 and the multiple storage frames 26 greatly increase the sample storage capacity, making access to biological samples more flexible and greatly improving the efficiency of accessing the sample tube 11.

[0062] In another embodiment, Figures 1-8 As shown, an inverted T-shaped slide groove 15 is provided inside the sample plate 14, an inverted T-shaped slider 16 is fixedly installed on the bottom of the movable seat 13, and the inverted T-shaped slider 16 is slidably installed on the inner side of the sample plate 14. An inverted T-shaped slide groove 23 is provided inside the rotating frame 19 and the storage frame 26, and the specifications and dimensions of the inverted T-shaped slide groove 23 are adapted to the specifications and dimensions of the inverted T-shaped slider 16.

[0063] The inverted T-shaped slide groove 15 and the inverted T-shaped slider 16 are provided to ensure the stability of the movable seat 13 moving on the top of the sample plate 14, and the inverted T-shaped slide groove 2 33 is provided to ensure the stability of the movable seat 13 moving inside the storage frame 26 and the rotating frame 19, thereby ensuring the stability of storing and removing the sample tube 11.

[0064] In another embodiment, Figures 1-8 As shown, an outer protective shell 2 is fixedly installed on the outside of the cabinet 1, and observation windows 3 are installed inside the outer protective shell 2 and the cabinet 1. Inspection doors 9 are installed on both sides of the cabinet 1. Heat dissipation vents 10 are opened on both sides of the cabinet 1, and the heat dissipation vents 10 correspond to the heat dissipation places of the programmed cooling device 23. An RFID scanner 5 is installed on the front of the cabinet 1, and a USB interface 6 is installed on the front of the cabinet 1.

[0065] The provided cabinet 1 facilitates the staff to observe the process of storing and removing the sample tubes 11, thereby ensuring the normal use of the device. The provided inspection door 9 facilitates the inspection of the equipment inside the cabinet 1, and the provided heat dissipation port 10 can discharge the heat generated when the program cooling device 23 is running.

[0066] The method for using the intelligent ultra-low temperature biological sample storage cabinet includes the following steps:

[0067] S1: Entering information: Before placing and storing the sample tube 11, the RFID tag on the outside of the sample tube 11 can be scanned by the RFID scanner 5, and the scanning result will be transmitted to the control box 22. In addition to the controller, the control box 22 also contains a processor, an information memory and a PC board. The processor inside the control box 22 can process the sample information of the sample tube 11, including the sample type, the expected storage location and the expected storage time, and transmit the information to the control display screen 7 for display after being processed by the processor inside the control box 22. The information of the sample tube 11 is stored in the information memory inside the control box 22, and the program cooling device 23 is turned on to cool the inside of the cabinet 1 so that the temperature inside the cabinet 1 is suitable for storing the sample inside the sample tube 11;

[0068] S2: Sample storage: When the sample tube 11 needs to be stored, the sample tube 11 can be placed on the top of the placement seat 12, and then the placement seat 12 is placed inside the movable seat 13, and then the movable seat 13 is placed on the top of the sample plate 14. Then, the command is input through the control display screen 7, and the command is transmitted to the processor inside the control box 22. Then the processor transmits the data to the controller, and the controller controls the adjustment cylinder 36 and the drive motor 38 to start. After the adjustment cylinder 36 is started, the output end retracts, thereby driving the connecting plate 44 to move upward, and the seal is pulled by the fixed connecting rod 47. The sealing slider 46 moves upward in the sealing groove 18. When the sample plate 14 approaches the intelligent sealing door 29, the intelligent sealing door 29 will automatically open, so that the sample plate 14 carrying the moving seat 13, the placement seat 12 and the sample tube 11 enter the interior of the cabinet 1. After entering, the intelligent sealing door 29 will automatically close to ensure the sealing and safety of the interior of the cabinet 1. In addition, when the connecting plate 44 moves upward, the moving frame 30 can be driven to move upward by the connecting plate 44. When the moving frame 30 moves upward, the moving block 49 and the pushing cylinder 50 will first move upward under the action of the elastic force of the spring 48. In a stationary state, the moving frame 30 will then pull the moving block 49 and the pushing electric cylinder 50 upwards through the spring 48, and make the position of the pushing plate 51 correspond to the height of the position of the moving seat 13. In addition, after the driving motor 38 is started, it can drive the rotating frame 19 to rotate, so that one of the storage frames 26 corresponds to the position of the sample plate 14. By adjusting the cooperation of the electric cylinder 36 and the driving motor 38, the sample plate 14 can be made to correspond to the position of one of the in-and-out slots 28 and the storage frame 26. Then, the pushing electric cylinder 50 is started, and the output end of the pushing electric cylinder 50 can push The push plate 1 51 is moved, thereby pushing the movable seat 13 away from the top of the sample plate 14 through the push plate 1 51, and pushing the movable seat 13 carrying the placement seat 12 and the sample tube 11 into the storage frame 26, thereby completing the storage of the sample tube 11. The inverted T-shaped slide groove 15 and the inverted T-shaped slider 16 can ensure the stability of the movable seat 13 moving on the top of the sample plate 14, and the inverted T-shaped slide groove 2 33 can ensure the stability of the movable seat 13 moving inside the storage frame 26 and the rotating frame 19, thereby ensuring the stability of storing and taking out the sample tube 11.

[0069] S3: Sample removal: When the output end of the control-adjusting electric cylinder 36 drives the sample plate 14 to move to the front of the storage frame 26 where the movable seat 13, the placement seat 12 and the sample tube 11 that need to be taken out are stored, the connecting plate 44 can also drive the movable frame 2 40 to move through the connecting rod 2 37, the connecting bending rod 24 and the connecting rod 1 34, so that the pushing electric cylinder 2 42 and the pushing plate 2 43 are aligned with the ejection port 35 on the back of the storage frame 26, and then the pushing electric cylinder 2 42 is started. The output end of the pushing electric cylinder 2 42 can push the pushing plate 2 43 to move, so that the movable seat 13 carrying the placement seat 12 and the sample tube 11 are pushed to the top of the sample plate 14 through the pushing plate 2 43, and then the output end of the adjusting electric cylinder 36 is started to extend, so that the sample plate 14 carrying the movable seat 13, the placement seat 12 and the sample tube 11 are close to the intelligent sealing door 29. The intelligent sealing door 29 opens automatically, allowing the sample plate 14 carrying the movable seat 13, the placement seat 12 and the sample tube 11 to be removed from the interior of the cabinet 1, making it convenient for the experimenter to perform sampling operations and being able to intelligently access the sample tube 11, greatly improving the work efficiency of the experimenter in accessing the sample tube 11. There is no need to manually open the cabinet door, avoiding outdoor operations and avoiding damage to the samples. The rotation of the rotating frame 19 and the multiple storage frames 26 greatly increase the sample storage capacity, making the access to biological samples more flexible, and greatly improving the efficiency of accessing the sample tube 11.

[0070] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. An intelligent ultra-low temperature biological sample storage cabinet, comprising a cabinet body (1) and a sample tube (11), characterized in that: A storage component is installed inside the cabinet (1), and a placement and removal component is installed inside the cabinet (1), and the placement and removal component is used to move the sample tube (11) into the interior of the storage component; The storage assembly includes a rotating placement assembly and a driving assembly for driving the rotating placement assembly to rotate. The rotating placement assembly includes a rotating frame (19). A plurality of inlet and outlet slots (28) are provided inside the frame wall of the rotating frame (19). A plurality of storage frames (26) are installed inside the rotating frame (19). The positions of the plurality of storage frames (26) correspond to the positions of the inlet and outlet slots (28). A push-out port (35) is provided on one side of each storage frame (26). The placing and removing component includes an adjusting electric cylinder (36), the output end of the adjusting electric cylinder (36) is fixedly installed with a connecting plate (44), the bottom of one end of the connecting plate (44) is fixedly installed with a limiting slide (45), one end of the limiting slide (45) is fixedly installed with a moving frame (30), the interior of the moving frame (30) is slidably installed with a moving block (49), the top of the moving block (49) is fixedly connected to two springs (48), and the top of the spring (48) is fixedly connected to the bottom of the inner cavity of the moving frame (30). A push cylinder (50) is fixedly installed on one side of the moving block (49), and a push plate (51) is fixedly installed on the output end of the push cylinder (50). An entry and exit frame (8) is installed on the front of the cabinet (1), and an inlet and outlet slot is provided on the top of the entry and exit frame (8). An intelligent sealing door (29) is installed on the top of the entry and exit frame (8), and a sealing slide (18) is provided on one side of the entry and exit frame (8). A sealing slide (46) is slidably installed inside the sealing slide (18). The sealing slide (4 6) is fixedly mounted on one side of the connecting block (17), a sample plate (14) is fixedly mounted on the top of the connecting block (17), a movable seat (13) is movably mounted on the top of the sample plate (14), a placement seat (12) is clamped and mounted on the top of the movable seat (13), a sample tube (11) is placed on the top of the placement seat (12), a fixed connecting rod (47) is fixedly mounted on the bottom of the connecting plate (44) away from the limiting slide (45), and one side of the bottom of the fixed connecting rod (47) is connected to the sealing slider (46) away from the sealing slider (46) One end of the block (17) is fixedly connected, and when the connecting plate (44) moves upward, the moving frame (30) can be driven to move upward by the connecting plate (44). When the moving frame (30) moves upward, the moving block (49) and the pushing electric cylinder (50) will first be in a stationary state under the action of the elastic force of the spring (48). Then, the moving frame (30) will pull the moving block (49) and the pushing electric cylinder (50) upward through the spring (48), and make the position of the pushing plate (51) correspond to the position height of the moving seat (13).

2. The intelligent ultra-low temperature biological sample storage cabinet according to claim 1, characterized in that: The driving assembly includes a support base (21), a driving motor (38) is fixedly installed inside the support base (21), an output end of the driving motor (38) is fixedly connected to the bottom of the rotating frame (19), a partition (20) is fixedly installed inside the cabinet (1), the support base (21) is fixedly installed on the top of the partition (20), the top and both sides of the storage frame (26) are provided with circular through holes (27), and a plurality of rectangular slot groups (25) are provided inside the rotating frame (19).

3. The intelligent ultra-low temperature biological sample storage cabinet according to claim 2, characterized in that: The rectangular slot groups (25) are arranged in a plurality of groups at intervals along the circumferential direction of the side wall of the rotating frame (19), and each rectangular slot group (25) includes a plurality of strip-shaped heat dissipation holes arranged longitudinally along the height direction of the rotating frame (19). A plurality of storage frames (26) are arranged from top to bottom on the inner wall of the rotating frame (19) between any two rectangular slot groups (25), and each storage frame (26) is in a cube shape. The storage frame (26) is arranged corresponding to the inlet and outlet slots (28). The driving motor (38) drives the rotating frame (19) to rotate. The driving motor (38) is provided with a first magnetic induction switch. A magnetic induction sheet is provided in the middle of the lower outer wall of each storage frame (26) located around the bottom of the rotating frame (19), and each magnetic induction sheet forms a unique position corresponding relationship with the plurality of storage frames (26) above the same column. A control box (22) is fixedly installed at the bottom of the inner cavity of the cabinet (1), and a controller is installed in the control box (22). The controller is electrically connected to the drive motor (38) and the first magnetic induction switch respectively. During the access process of the sample tube (11), after determining the target storage frame (26) corresponding to the access of the sample tube (11), the corresponding magnetic induction piece is locked according to the position of the target storage frame (26). The controller controls the drive motor (38) to drive the rotating frame (19) to rotate. At the same time, during the rotation process, the first magnetic induction switch senses the signal of each magnetic induction piece in real time. When the first magnetic induction switch senses the corresponding magnetic induction piece, the controller determines that the target storage frame (26) has been reached and controls the drive motor (38) to stop working.

4. The intelligent ultra-low temperature biological sample storage cabinet according to claim 3 is characterized by: A switch button (4) is installed on the front of the cabinet (1), and a control display screen (7) is installed on the front of the cabinet (1).

5. The intelligent ultra-low temperature biological sample storage cabinet according to claim 4 is characterized in that: The top of the connecting plate (44) away from the limiting slide (45) is fixedly mounted with a connecting rod 2 (37), the top of the connecting rod 2 (37) is fixedly mounted with a connecting bending rod (24), the bottom of the connecting bending rod (24) away from the end of the connecting rod 2 (37) is fixedly mounted with a connecting rod 1 (34), the bottom of the connecting rod 1 (34) is fixedly mounted with a moving frame 2 (40), the interior of the moving frame 2 (40) is slidably mounted with a moving block 2 (41), the top of the moving block 2 (41) is fixedly connected with two springs 2 (39), and the top of the spring 2 (39) is fixedly connected to the bottom of the inner cavity of the moving frame 2 (40), one side of the moving block 2 (41) is fixedly mounted with a pushing electric cylinder 2 (42), the output end of the pushing electric cylinder 2 (42) is fixedly mounted with a pushing plate 2 (43), and the moving frame 2 (40) and the pushing electric cylinder 2 (42) are both located on the inner side of the rotating frame (19).

6. The intelligent ultra-low temperature biological sample storage cabinet according to claim 5, characterized in that: A vertical plate (31) is fixedly installed inside the cabinet (1), a limiting slide groove (32) is provided inside the vertical plate (31), the limiting slide plate (45) is slidably installed inside the limiting slide groove (32), and an elastic sealing belt is installed inside the sealing slide groove (18), and the elastic sealing belt is fixedly connected to the bottom of the sealing slide block (46).

7. The intelligent ultra-low temperature biological sample storage cabinet according to claim 6, characterized in that: The sample plate (14) is provided with an inverted T-shaped slide groove (15) inside, and an inverted T-shaped slider (16) is fixedly installed on the bottom of the movable seat (13). The inverted T-shaped slider (16) is slidably installed on the inner side of the sample plate (14). The rotating frame (19) and the storage frame (26) are both provided with an inverted T-shaped slide groove (33) inside, and the specifications and dimensions of the inverted T-shaped slide groove (33) are adapted to the specifications and dimensions of the inverted T-shaped slider (16).

8. The intelligent ultra-low temperature biological sample storage cabinet according to claim 7, characterized in that: An outer protective shell (2) is fixedly mounted on the outer side of the cabinet (1), and observation windows (3) are mounted inside the outer protective shell (2) and the cabinet (1). Inspection doors (9) are mounted on both sides of the cabinet (1), and heat dissipation ports (10) are provided on both sides of the cabinet (1). Programmed cooling devices (23) are mounted on both sides of the inner cavity of the cabinet (1), and the programmed cooling devices (23) cool the interior of the cabinet (1). The heat dissipation ports (10) correspond to the heat dissipation locations of the programmed cooling devices (23). An RFID scanner (5) is mounted on the front of the cabinet (1), and a USB interface (6) is mounted on the front of the cabinet (1).

9. The intelligent ultra-low temperature biological sample storage cabinet according to claim 8, characterized in that: The rotating frame (19) is provided with a plurality of temperature sensors mounted on its wall. The controller is electrically connected to the programmed cooling device (23) and the temperature sensors, respectively. The controller is used to control the temperature of the programmed cooling device (23), including: Obtaining a start control instruction of a program cooling device (23); The program cooling device (23) is started according to the program cooling device (23) start control instruction, and the response time of the program cooling device (23) start control instruction is obtained; When the program cooling device (23) cools the sample tube (11) inside the cabinet (1), temperature change information of the temperature sensor is obtained in real time; Analyze the temperature change information of the temperature sensor to determine the temperature change characteristics inside the cabinet (1); Performing a cooling temperature prediction based on the temperature variation characteristics inside the cabinet (1) to obtain cooling prediction information inside the cabinet (1); Determine the target analysis time according to the response time of the control instruction of the program cooling device (23), analyze the temperature reduction prediction information in the cabinet (1) in combination with the target analysis time, determine whether the internal temperature of the cabinet (1) reaches the minimum internal temperature of the cabinet (1) after the target analysis time, and obtain the prediction analysis result; When the predicted analysis result shows that the temperature inside the cabinet (1) does not reach the minimum temperature inside the cabinet (1) after the target analysis time, the programmed cooling device (23) continues to cool the inside of the cabinet (1); When the predicted analysis result shows that the temperature inside the cabinet (1) reaches the minimum temperature inside the cabinet (1) after the target analysis time, a pause control instruction of the program cooling device (23) is obtained, and the program cooling device (23) is stopped to cool the inside of the cabinet (1).

10. A method for using the intelligent ultra-low temperature biological sample storage cabinet according to claim 9, characterized in that: The following steps are involved: S1: Inputting information: Before the sample tube (11) is placed for storage, the RFID tag on the outside of the sample tube (11) is scanned by the RFID scanner (5), and the scanning result is transmitted to the control box (22). The processor inside the control box (22) processes the sample information of the sample tube (11) and starts the program cooling device (23) to cool the inside of the cabinet (1) so that the temperature inside the cabinet (1) is suitable for storing the sample inside the sample tube (11); S2: Sample storage: First, place the sample tube (11) on the top of the placement seat (12), then input a command through the control display screen (7), so that the adjustment electric cylinder (36) inside the placement and removal component is started and the output end is retracted, so that the sample tube (11) enters the interior of the cabinet (1), and the driving motor (38) is started to drive the rotating frame (19) to rotate. By cooperating with the adjustment electric cylinder (36) and the driving motor (38), the sample plate (14) can be aligned with the position of one of the inlet and outlet slots (28) and the storage frame (26), and then the pushing electric cylinder (50) is started to embed the movable seat (13) carrying the placement seat (12) and the sample tube (11) into the interior of the storage frame (26), thereby completing the storage of the sample tube (11); S3: Sample removal: When the output end of the control-adjusting electric cylinder (36) drives the sample plate (14) to move to the front of the storage frame (26) where the sample tube (11) to be removed is stored, the pushing electric cylinder 2 (42) is started. The output end of the pushing electric cylinder 2 (42) can push the pushing plate 2 (43) to move, thereby pushing the moving seat (13) carrying the placement seat (12) and the sample tube (11) to the top of the sample plate (14), and then the output end of the adjusting electric cylinder (36) is started to extend, thereby removing the sample tube (11) from the inside of the cabinet (1).

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