A self-sustaining biobank device

Abstract

The application discloses a self-maintaining biological sample bank device, which is applied to the field of biological sample storage and comprises a storage device frame, a micro-positive pressure cavity is connected to the top of the storage device frame, a biological liquid nitrogen tank is connected to the middle of the storage device frame, a transfer box is connected to the middle of the storage device frame, a transfer interface is connected to the middle of the storage device frame close to the transfer box, and the micro-positive pressure cavity is communicated with the biological liquid nitrogen tank through the transfer box; the storage device frame, the micro-positive pressure cavity, the electric control cabinet, the biological liquid nitrogen tank, the transfer box and the transfer interface are arranged to form a self-maintaining structure of the biological sample bank, the function of deep low-temperature storage of biological samples is realized, the problem that a traditional liquid nitrogen tank needs to be regularly added with liquid nitrogen to supplement a cold source is solved, and the operation of replacing a liquid nitrogen tower, a large liquid nitrogen generating device, a liquid nitrogen supplement tank and manually supplementing liquid nitrogen is replaced.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of biological sample storage, in particular to a self-stabilized biological sample bank equipment. BACKGROUND

[0002] The biological sample bank is an important basic equipment for life science research, which bears the functions of collecting, processing, storing, monitoring and information management of biological samples. Its main equipment includes storage equipment, processing equipment, monitoring equipment, auxiliary equipment, information equipment and security equipment, etc. The storage equipment includes liquid nitrogen tank, ultra-low temperature refrigerator and automatic storage system.

[0003] At present, the patent with the patent number CN206427590U, named full-automatic ultra-low temperature honeycomb type biological sample bank, discloses a full-automatic ultra-low temperature honeycomb type biological sample bank, which comprises: a heat preservation cold storage system; a honeycomb type liquid nitrogen tank for storing biological samples and arranged in the heat preservation cold storage system; a transfer cold tank system for temporarily storing biological samples to be taken out from the honeycomb type liquid nitrogen tank or temporarily storing biological samples to be stored in the honeycomb type liquid nitrogen tank, and the transfer cold tank system is arranged in the heat preservation cold storage system; an automatic access tube integrated system for storing the temporarily stored biological samples in the transfer cold tank system into the honeycomb type liquid nitrogen tank and storing the biological samples to be taken out from the honeycomb type liquid nitrogen tank into the transfer cold tank system; an integrated controller electrically connected with the automatic access tube integrated system. The technical scheme realizes the full-automatic storage process of single sample access, eliminates the safety hazards to the operators, and realizes the full cold chain transportation in the access process, which guarantees the cell activity in the sample.

[0004] However, when storing samples, the device uses a liquid nitrogen supplement device for temperature control. The liquid nitrogen tank needs to be supplemented with liquid nitrogen during operation, and the operation steps are complicated. SUMMARY

[0005] The purpose of the present application is to provide a self-stabilized biological sample bank equipment, which has the advantages of building a deep low-temperature refrigeration system to maintain the low-temperature environment in the liquid nitrogen tank, realizing the replacement of manual operation for storing and selecting biological samples, replacing the periodic addition of liquid nitrogen in the traditional liquid nitrogen container, supplementing the cold source, solving the problem that the biological sample bank needs to rely on stable liquid nitrogen supply, and the sample bank does not need to be equipped with a large-scale infrastructure such as a liquid nitrogen tower. The construction of biological sample bank in western remote areas and special geographical locations is no longer limited, and the safety of biological sample storage is guaranteed.

[0006] The above technical objective of the present application is achieved by the following technical scheme: a storage device frame is provided, a micro-positive pressure cavity is connected to the top of the storage device frame, a biological liquid nitrogen tank is connected to the middle of the storage device frame, a transfer box is connected to the middle of the storage device frame, a transfer interface is connected to the middle of the storage device frame close to the transfer box, the micro-positive pressure cavity is communicated with the biological liquid nitrogen tank, a corresponding cover is arranged on the tank opening of the biological liquid nitrogen tank, and the cover covers the liquid nitrogen tank when the device is in a dormant state, thereby reducing the volatilization of liquid nitrogen; a deep low-temperature refrigeration assembly is arranged on the top of the storage device frame close to the micro-positive pressure cavity, an electric control cabinet is connected to the top of the storage device frame close to the micro-positive pressure cavity, and an automatic biological sample storage mechanism is arranged in the micro-positive pressure cavity.

[0007] By arranging the storage device frame, the micro-positive pressure cavity, the electric control cabinet, the biological liquid nitrogen tank, the transfer box, the transfer interface and the automatic storage and retrieval device, a self-maintenance structure of the biological sample library is formed, the function of automatically storing the biological sample at a deep low temperature is realized, the problem that the traditional biological sample library liquid nitrogen tank needs to be regularly supplemented with consumable liquid nitrogen to supplement the cold source is solved, and the replacement of the liquid nitrogen tower, the large liquid nitrogen generating device, the liquid nitrogen supplement tank and the manual operation to supplement the liquid nitrogen is replaced.

[0008] The present application further provides that the deep low-temperature refrigeration assembly comprises an expansion container, a capillary tube and a Stirling refrigerator, the Stirling refrigerator is connected to the top of the storage device frame close to the micro-positive pressure cavity, the expansion container is welded to the top of the storage device frame close to the micro-positive pressure cavity, and the capillary tube is welded to the output end of the Stirling refrigerator and the side wall of the expansion container.

[0009] By arranging the expansion container, the capillary tube and the Stirling refrigerator, a deep low-temperature refrigeration system is formed, the function of delivering cold energy to the biological liquid nitrogen tank is realized, the situation that the temperature rises due to the loss of cold energy in the biological liquid nitrogen tank is solved, the low-temperature environment in the biological liquid nitrogen tank is maintained, and the safety of the biological sample storage is improved. At the same time, the cold source of the deep low-temperature refrigeration system is directly installed in the biological liquid nitrogen tank, the nitrogen gas evaporated from the liquid nitrogen in the tank is liquefied, the loss of liquid nitrogen in the tank is reduced, and ultimately the loss of liquid nitrogen is zero.

[0010] The present application further provides that a liquid nitrogen generator is connected inside the storage device frame, and the liquid nitrogen generator is communicated with the biological liquid nitrogen tank.

[0011] By arranging the liquid nitrogen generator, an additional cold backup of the device is formed, the function of delivering liquid nitrogen to the biological liquid nitrogen tank in an emergency is realized, the situation that the cold energy in the biological liquid nitrogen tank cannot be supplemented when the Stirling refrigerator abnormally works and the liquid level in the liquid nitrogen tank is abnormal is solved, and the safety of the biological sample storage is improved.

[0012] The application is further provided with: the biological liquid nitrogen tank and the micro-positive pressure cavity are communicated, and a pressure relief valve is installed on the top of the micro-positive pressure cavity.

[0013] By the above technical scheme, the micro-positive pressure cavity is isolated from the outside, the water vapor entering the micro-positive pressure cavity is solved, and the icing and frosting in the micro-positive pressure cavity is prevented.

[0014] The application is further provided with: the storage equipment frame is connected with a UPS backup power supply away from the inside of the biological liquid nitrogen tank, and the UPS backup power supply is connected with the electric control cabinet.

[0015] By the above technical scheme, the equipment backup power supply is formed, the UPS backup power supply is connected to the equipment after power failure to provide temporary power supply for the equipment, the current access task is ensured to be completed, the liquid nitrogen tank cover is closed, and an alarm is sent to relevant personnel and departments, thereby improving the safety of biological sample storage.

[0016] The application is further provided with: an outer shell is connected to the outer wall of the storage equipment frame, and an oxygen concentration detector is installed on the storage equipment frame through the shell.

[0017] By the above technical scheme, the oxygen concentration detector is used to realize the function of oxygen concentration detection of the equipment, improve the stability of the equipment during work, and timely alarm when the equipment state is abnormal.

[0018] The application is further provided with: a plurality of adjusting lead screws are symmetrically arranged on the bottom of the storage equipment frame, and support foot plates are welded to the bottom ends of the adjusting lead screws.

[0019] By the above technical scheme, the adjusting lead screw and the support foot plate are used to form an adjustable support structure, realize the function of supporting and fixing the equipment, solve the problem of equipment shaking caused by unstable placement, and improve the stability of the equipment during work.

[0020] The application is further provided with: a plurality of universal wheels are symmetrically arranged on the bottom of the storage equipment frame.

[0021] By the above technical scheme, the universal wheel is used to form a moving structure, realize the function of moving the equipment, and improve the convenience of equipment movement.

[0022] In summary, the application has the following advantages:

[0023] 1. The present application builds a deep cryogenic refrigeration system by setting a liquid nitrogen tank, an expansion vessel, a capillary tube and a Stirling refrigerator inside a storage device frame to ensure the low temperature environment in the liquid nitrogen tank, realizes the replacement of artificial operation storage and selection of biological samples, replaces the traditional liquid nitrogen container which often needs to add liquid nitrogen and supplement the cold source, reduces the situation of biological sample bank equipped with liquid nitrogen tower, and guarantees the safety of biological sample storage.

[0024] 2. The present application comprises a liquid nitrogen uniform spraying mechanism composed of a lead screw, a guide rod, a sliding plate, a fixed plate and a first nozzle, realizes the uniform spraying of liquid nitrogen in the micro-positive pressure cavity, increases the uniformity of temperature reduction in each part of the micro-positive pressure cavity, solves the non-uniformity of liquid nitrogen in the micro-positive pressure cavity when cooling, and improves the quality of biological sample storage. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 is the overall structure schematic diagram in the present application;

[0026] Figure 2 is the left view of the overall structure in the present application;

[0027] Figure 3 is the right view of the overall structure in the present application;

[0028] Figure 4 is the top view of the overall structure in the present application.

[0029] Reference signs: 1, storage device frame; 11, micro-positive pressure cavity; 12, electric control cabinet; 14, biological liquid nitrogen tank; 15, expansion vessel; 16, capillary tube; 17, Stirling refrigerator; 18, transfer box; 19, transfer interface; 2, liquid nitrogen making machine; 3, UPS backup power supply; 4, adjusting lead screw; 41, supporting foot disc; 5, universal wheel. DETAILED DESCRIPTION

[0030] The present application will be further described in detail below in combination with the drawings.

[0031] Example 1:

[0032] Reference Figures 1 to 4, including a storage device frame 1, a micro-positive pressure cavity 11 connected to the top of the storage device frame 1, a biological liquid nitrogen tank 14 connected to the middle of the storage device frame 1, a transfer box 18 connected to the middle of the storage device frame 1, a transfer interface 19 connected to the middle of the storage device frame 1 close to the transfer box 18, the micro-positive pressure cavity 11 being in communication with the biological liquid nitrogen tank 14, the biological liquid nitrogen tank 14 having a corresponding cover on the tank opening, the cover being placed on the liquid nitrogen tank during device hibernation to reduce liquid nitrogen evaporation, a deep low temperature refrigeration assembly being provided on the top of the storage device frame 1 close to the micro-positive pressure cavity 11, an electric control cabinet 12 being connected to the top of the storage device frame 1 close to the micro-positive pressure cavity 11, and an automatic biological sample storage mechanism being provided inside the micro-positive pressure cavity 11; in operation, liquid nitrogen is filled in the biological liquid nitrogen tank 14 for cooling at the first use, after cooling, the deep low temperature refrigeration assembly is started to transfer cold energy to the biological liquid nitrogen tank 14, thereby maintaining a low temperature environment in the biological liquid nitrogen tank 14, the biological sample is transferred from the micro-positive pressure cavity 11 to the biological liquid nitrogen tank 14 for storage through the transfer box 18 and the transfer interface 19, and the electric control cabinet 12 is a control system inside the device for controlling stable operation of the device; this step forms a biological sample library self-maintenance structure through the settings of the storage device frame 1, the micro-positive pressure cavity 11, the electric control cabinet 12, the biological liquid nitrogen tank 14, the transfer box 18 and the transfer interface 19, realizes the function of deep low temperature storage of the biological sample, solves the problem of regular addition of liquid nitrogen to supplement the cold source of the traditional liquid nitrogen tank, and replaces the liquid nitrogen tower, the large liquid nitrogen generating device, the liquid nitrogen supplement tank and the manual operation of supplementing liquid nitrogen.

[0033] Reference Figure 1 , the deep low temperature refrigeration assembly includes an expansion container 15, a capillary tube 16 and a Stirling refrigerator 17, the Stirling refrigerator 17 being connected to the top of the storage device frame 1 close to the micro-positive pressure cavity 11; the expansion container 15 being welded to the top of the storage device frame 1 close to the micro-positive pressure cavity 11, and the capillary tube 16 having two ends respectively welded to the output end of the Stirling refrigerator 17 and the side wall of the expansion container 15; in operation, the Stirling refrigerator 17, the capillary tube 16, the expansion container 15 and a plurality of electromagnetic valves build a deep low temperature refrigeration system to transport cold energy to the biological liquid nitrogen tank 14, and liquefy the nitrogen gas evaporated from the liquid nitrogen in the biological liquid nitrogen tank 14 into liquid nitrogen; this step forms a deep low temperature refrigeration system through the settings of the expansion container 15, the capillary tube 16 and the Stirling refrigerator 17, realizes the function of transporting cold energy to the biological liquid nitrogen tank 14, solves the problem of temperature rise caused by cold energy loss in the biological liquid nitrogen tank 14, maintains a low temperature environment in the biological liquid nitrogen tank 14, and improves the safety of biological sample preservation.

[0034] Reference Figures 1 to 3The inside of the storage equipment frame 1 is connected with a liquid nitrogen making machine 2, and the liquid nitrogen making machine 2 communicates with the biological liquid nitrogen tank 14; in work, the liquid nitrogen making machine 2 transports liquid nitrogen into the biological liquid nitrogen tank 14 according to the liquid level and temperature feedback of the liquid nitrogen in the biological liquid nitrogen tank 14, and the cold energy in the biological liquid nitrogen tank 14 is supplemented by the Stirling refrigerator 17 under the condition that the Stirling refrigerator 17 works normally, and the liquid nitrogen making machine 2 serves as an additional cold backup; through the setting of the liquid nitrogen making machine 2, the equipment additional cold backup is formed, the function of transporting liquid nitrogen into the biological liquid nitrogen tank 14 in an emergency is realized, the situation that the cold energy in the biological liquid nitrogen tank 14 cannot be supplemented when the Stirling refrigerator 17 abnormally works is solved, and the safety of biological sample storage is improved.

[0035] With reference to Figure 1 and Figure 2 The biological liquid nitrogen tank 14 communicates with the micro-positive pressure cavity 11, and the top of the micro-positive pressure cavity 11 is welded with a pressure relief valve; in work, the high-purity nitrogen gas volatilized in the biological liquid nitrogen tank 14 diffuses in the micro-positive pressure cavity 11, the air in the micro-positive pressure cavity 11 is exhausted, and at the same time, the dehumidification and atmosphere protection are performed, the micro-positive pressure cavity 11 adopts high-efficiency sealing means, so that the external air cannot enter the cavity, and the pressure relief valve ensures the safety of the pressure in the micro-positive pressure cavity 11; through the communication between the biological liquid nitrogen tank 14 and the micro-positive pressure cavity 11 and the setting of the pressure relief valve, the inside of the micro-positive pressure cavity 11 is isolated from the outside, the water vapor entering the micro-positive pressure cavity 11 is reduced, the icing and frosting in the micro-positive pressure cavity 11 is reduced, the information acquisition device in the micro-positive pressure cavity 11 is not disturbed by water vapor, and the accuracy of scanning information of the information acquisition device in the micro-positive pressure cavity 11 is improved.

[0036] With reference to Figure 2 The inside of the storage equipment frame 1 far from the biological liquid nitrogen tank 14 is connected with a UPS backup power supply 3, and the UPS backup power supply 3 is connected with the electric control cabinet 12; in work, when the main power supply of the equipment is offline, the power supply is switched to the UPS backup power supply 3, and the UPS backup power supply 3 supplies power to the equipment for a short time; through the setting of the UPS backup power supply 3, the equipment backup power supply is formed, the UPS backup power supply 3 is connected to the equipment to supply power to the equipment for a short time after the equipment is powered off, the situation that the temperature in the biological liquid nitrogen tank 14 rises due to the stop of the Stirling refrigerator 17 caused by the power-off of the equipment is reduced, and the safety of biological sample storage is improved.

[0037] With reference to Figure 1 The outside wall of the storage equipment frame 1 is connected with a shell, and the storage equipment frame 1 is installed with an oxygen concentration detector through the shell; in work, the oxygen concentration detector detects the oxygen concentration of the equipment, and an alarm information is generated when the oxygen concentration exceeds the specified range; through the oxygen concentration detector, the function of detecting the oxygen concentration of the equipment is realized, the stability of the equipment in work is improved, and the function of timely generating an alarm when the state of the equipment is abnormal is realized.

[0038] With reference toFigure 2 And Figure 3 The storage equipment frame 1 is provided with a plurality of groups of adjusting lead screws 4 at the bottom, which are symmetrically arranged, and the bottom end of each adjusting lead screw 4 is welded with a support foot plate 41; in work, after the equipment is placed in the working area, the adjusting lead screw 4 is screwed to adjust the height of the support foot plate 41, so that the support foot plate 41 is in contact with the working surface to support and fix the equipment; this step forms an adjustable support structure through the adjusting lead screw 4 and the support foot plate 41, realizes the function of supporting and fixing the equipment, solves the problem of equipment shaking caused by unstable placement, and improves the stability of the equipment in work.

[0039] Reference Figure 2 The storage equipment frame 1 is provided with a plurality of groups of universal wheels 5 at the bottom, which are symmetrically arranged; in work, when the equipment needs to be moved, the universal wheels 5 are used to move the equipment; this step forms a moving structure through the universal wheels 5, realizes the function of moving the equipment, and improves the convenience of moving the equipment.

[0040] Reference Figure 1 The storage equipment frame 1 is made of aluminum alloy; in work, the storage equipment frame 1 made of aluminum alloy increases the structural strength of the storage equipment frame 1, reduces the overall quality of the equipment, and reduces the erosion and damage of the storage equipment frame 1.

[0041] Brief description of use process: working principle: in the work, the biological liquid nitrogen tank 14 is filled with liquid nitrogen for cooling at first use, after cooling, the deep low temperature refrigeration assembly is started to transfer cold quantity in the biological liquid nitrogen tank 14, so as to maintain the low temperature environment in the biological liquid nitrogen tank 14, the biological sample is transferred from the micro positive pressure cavity 11 to the biological liquid nitrogen tank 14 for storage through the transfer box 18 and the transfer interface 19, the electric control cabinet 12 is the control system inside the equipment, which is used to control the stable operation of the equipment, the stirling refrigerator 17, the capillary 16, the expansion container 15 and the equipped several electromagnetic valves build the deep low temperature refrigeration system, which transports the cold quantity in the biological liquid nitrogen tank 14, liquefies the nitrogen gas evaporated from the biological liquid nitrogen tank 14 into liquid nitrogen, the liquid nitrogen machine 2 transports liquid nitrogen into the biological liquid nitrogen tank 14 according to the liquid level and temperature feedback of the liquid nitrogen in the biological liquid nitrogen tank 14, under the condition that the stirling refrigerator 17 works normally, the cold quantity in the biological liquid nitrogen tank 14 is supplemented by the stirling refrigerator 17, the liquid nitrogen machine 2 is an additional cold backup, the volatile high purity nitrogen gas in the biological liquid nitrogen tank 14 diffuses in the micro positive pressure cavity 11, the air in the micro positive pressure cavity 11 is exhausted, and the dehumidification and atmosphere protection are carried out, the micro positive pressure cavity 11 adopts high efficient sealing means, so that the external air cannot enter the cavity, the pressure relief valve ensures the safety of the pressure in the micro positive pressure cavity 11, when the main power of the equipment is offline, the power supply is switched to the UPS standby power supply 3, the UPS standby power supply 3 supplies power to the equipment for a short time, the oxygen concentration detector detects the oxygen concentration of the equipment, and when the oxygen concentration exceeds the specified range, an alarm information is generated, after the equipment is placed in the working area, the adjusting screw rod 4 is screwed, the height of the supporting foot plate 41 is adjusted, the supporting foot plate 41 is in contact with the working surface, the equipment is supported and fixed, when the equipment needs to be moved, the equipment is moved through the universal wheel 5.

[0042] The specific embodiment is only an explanation of the application, which is not a limitation of the application, and those skilled in the art can make modifications to the embodiment without creative contribution according to the needs after reading the specification, but as long as it is within the scope of the claims of the application, it is protected by the patent law.

Claims

1. A self-stabilizing biobank device, comprising a storage device frame (1), characterized in that, The storage device frame (1) is equipped with a micro-positive pressure cavity (11) on top. The storage device frame (1) is connected to a biological liquid nitrogen tank (14) in the middle. The storage device frame (1) has an opening on top corresponding to the position of the biological liquid nitrogen tank. The top platform of the frame is sealed to the opening of the biological liquid nitrogen tank. The storage device frame (1) is connected to a transfer box (18) in the middle. The storage device frame (1) is equipped with a transfer interface (19) near the transfer box (18). The micro-positive pressure cavity (11) is connected to the biological liquid nitrogen tank (14). The opening of the biological liquid nitrogen tank (14) is equipped with a corresponding lid. When the device is in hibernation, the lid is placed on the liquid nitrogen tank to reduce liquid nitrogen evaporation. The storage device frame (1) is equipped with a cryogenic refrigeration component near the top of the micro-positive pressure cavity (11). The storage device frame (1) is connected to an electrical control cabinet (12) near the top of the micro-positive pressure cavity (11). The micro-positive pressure cavity (11) is equipped with an automated biological sample storage mechanism. The cryogenic refrigeration assembly includes an expansion container (15), a capillary tube (16), and a Stirling refrigerator (17). The Stirling refrigerator (17) is connected to the top of the storage device frame (1) near the micro-positive pressure chamber (11) via a bracket. The expansion container (15) is riveted to the storage device frame (1) near the micro-positive pressure chamber (11). The two ends of the capillary tube (16) are respectively welded to the heat exchanger output end of the Stirling refrigerator (17) and the side wall of the expansion container (15). The storage device frame (1) is equipped with a liquid nitrogen generator (2), which is connected to the biological liquid nitrogen tank (14) by a pipeline and controlled by a solenoid valve. The biological liquid nitrogen tank (14) is connected to the micro-positive pressure chamber (11), and a pressure relief valve is installed on the top of the micro-positive pressure chamber (11); The storage device frame (1) is equipped with a UPS backup power supply (3) located inside the biological liquid nitrogen tank (14) away from the storage device frame (1). The UPS backup power supply (3) is connected to the electrical control cabinet (12).

2. The self-stabilizing biobank device according to claim 1, characterized in that, The outer wall of the storage device frame (1) is connected to a shell, and an oxygen concentration detector is installed in the storage device frame (1) through the shell.

3. The self-stabilizing biobank device according to claim 1, characterized in that, The storage device frame (1) is threadedly connected to an adjusting screw (4) at its bottom. Multiple sets of adjusting screws (4) are provided at the bottom of the storage device frame (1) and are arranged symmetrically. A support foot plate (41) is welded to the bottom end of the adjusting screw (4).

4. The self-stabilizing biobank device according to claim 1, characterized in that, The storage device frame (1) is connected to a universal wheel (5) at the bottom. Multiple sets of the universal wheels (5) are arranged symmetrically at the bottom of the storage device frame (1).

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