Giant salamander liver peptide composition formula

Abstract

The invention belongs to the field of biology, and particularly relates to a giant salamander liver peptide composition formula which comprises the following raw materials in parts by weight: 10-20 parts of giant salamander liver peptide liquid; 50 to 150 parts of ginseng; 20 to 40 parts of saussurea involucrata culture; 1 to 5 parts of yeast beta-glucan; 30 to 60 parts of radix puerariae; 40 to 60 parts of mulberry leaf; 35 to 60 parts of bitter gourd; 40 to 60 parts of poria cocos; the giant salamander liver peptide liquid is very easy to absorb due to the fact that the proportion of protein and amino acid is similar to that of a human body, can quickly act, and accelerates to achieve the effects of dispelling the effects of alcohol and protecting the liver. Wherein rich lecithin can regulate serum lipid, reduce cholesterol, prevent fatty liver, protect liver, improve memory and enhance immunity. In addition, the radix puerariae can enhance the activity of acetaldehyde reductase and accelerate alcohol metabolism, and has a synergistic effect with the giant salamander liver peptide liquid, so that the capabilities of dispelling the effects of alcohol and protecting the liver are improved, the absorptivity of a human body to the composition is also improved, and the health of drinkers is comprehensively protected.

Description

Technical Field

[0001] The invention belongs to the biological field, and specifically is a formula of a giant salamander liver peptide composition. Background Art

[0002] In modern social and life scenarios, drinking is extremely common. Drinking in moderation can, to a certain extent, promote metabolism, accelerate blood circulation, make people happy, and even have a positive significance for strengthening physical fitness. However, once drunkenness or excessive drinking occurs, it will bring many negative effects to physical health.

[0003] As an important metabolic organ in the human body, the liver plays a core role in the alcohol metabolism process. Excessive drinking will cause the metabolic pressure of the liver to increase exponentially. Acetaldehyde, the metabolite of alcohol in the liver, is highly toxic and can cause serious oxidative damage to liver cells. Over time, the normal function of liver cells is disturbed, fat metabolism becomes disordered, and a large amount of fat accumulates in the liver, which in turn causes fatty liver. If the situation continues to deteriorate, the liver tissue will gradually become fibrotic and may eventually develop into cirrhosis, which seriously threatens life and health. According to relevant medical research data, the incidence of alcoholic liver disease in people who drink excessively for a long time is as high as 30%-50%, and it is increasing year by year.

[0004] The giant salamander, belonging to the phylum Chordata and class Amphibia, once experienced a sharp decline in its population due to overfishing and other reasons, but is now listed as a national second-class protected animal. Since the 1990s, Hunan, Hubei, Shaanxi, Guangxi and other places have actively responded to the policy of protecting and rationally utilizing giant salamander resources, vigorously developed the giant salamander domestication and breeding industry, and established many large-scale domestication and breeding enterprises. After years of development, the annual reproductive capacity of giant salamanders has climbed to 600,000, and the scale of breeding is growing. But the problem that follows is how to carry out intensive processing and utilization of giant salamander resources, which has become a key and urgent issue related to whether the giant salamander industry can develop sustainably and healthily. The giant salamander itself is rich in a variety of bioactive substances, and there is great potential for the exploration and application of its effective ingredients.

[0005] In the existing hangover relieving product market, when people are drunk, they usually choose to take various hangover relieving products to relieve discomfort and speed up sobering up. However, these products generally have limitations. Most of them can only promote alcohol metabolism and cannot protect the liver while sobering up. What is more prominent is that the human body has poor absorption of these traditional hangover relieving products, which greatly reduces the effectiveness of the products. On the one hand, the ingredients of some products are complex and difficult to be quickly decomposed and absorbed by the human digestive system; on the other hand, the product formula lacks specificity and cannot accurately match the physiological mechanism of the human body to sober up and protect the liver.

[0006] To this end, the present invention provides a formula of a giant salamander liver peptide composition. Summary of the invention

[0007] In order to make up for the deficiencies of the prior art, at least one technical problem raised in the background technology is solved.

[0008] The technical solution adopted by the present invention to solve the technical problem is: the formula of the giant salamander liver peptide composition of the present invention is characterized in that the formula is composed of the following raw materials in proportion by weight:

[0009] 10 to 20 portions of giant salamander liver peptide liquid;

[0010] 50 to 150 parts of ginseng;

[0011] 20 to 40 copies of snow lotus culture;

[0012] Yeast β-glucan 1 to 5 parts;

[0013] 30 to 60 parts of Pueraria root;

[0014] 40 to 60 parts of mulberry leaves;

[0015] 35 to 60 servings of bitter melon;

[0016] 40 to 60 parts of Poria cocos;

[0017] 20 to 40 parts of honey.

[0018] A preparation process of a giant salamander liver peptide composition formula, the preparation process comprising the following steps:

[0019] S1. Raw material preparation: Select fresh and healthy liver tissue of artificially cultured giant salamander as the starting material, and ensure that the raw material is free of lesions and pollution;

[0020] S2. Tissue pounding: Put the selected liver tissue of giant salamander into a tissue pounder, and beat it into a fine and uniform meat paste by a high-speed rotating blade for subsequent processing;

[0021] S3, high temperature and high pressure aging: transfer the beaten minced meat to the high temperature and high pressure equipment; set the equipment parameters, and perform aging treatment on the protein tissue in the minced meat for 10 minutes under a high temperature of 121°C and a corresponding high pressure environment; this process can moderately denature the protein structure, which is more conducive to the subsequent enzymatic hydrolysis reaction;

[0022] S4. Material cooling: After the aging treatment is completed, the material is taken out from the high temperature and high pressure equipment; a combination of air cooling and water cooling can be used to quickly reduce the material temperature to 50°C and keep the temperature stable to create suitable conditions for the enzymatic reaction;

[0023] S5, enzymatic hydrolysis reaction: adding compound enzyme to the material cooled to 50°C according to the precise ratio of material to liquid of 1:4; during the enzymatic hydrolysis process, maintaining the temperature of the reaction system at a constant 50°C and maintaining the natural pH value without additional acid-base adjustment;

[0024] Based on the quality of fresh meat, the enzyme addition amount is strictly controlled at 2000-4000U / g to ensure that the content of compound enzyme in the entire reaction system is stably maintained at 0.1%-0.15%. The enzymatic reaction is continued for 4-8 hours, during which the compound enzyme fully acts to gradually degrade the large molecular protein into small molecular peptide substances;

[0025] S6, enzyme inactivation treatment: After the enzymatic reaction is completed, in order to prevent the enzyme from continuing to act and causing excessive enzymatic hydrolysis, the material is quickly placed in boiling water at 100°C for 15 minutes to completely terminate the enzyme activity;

[0026] S7, cooling to room temperature: After the enzyme is inactivated, the material is cooled naturally or by appropriate cooling measures to reduce its temperature to room temperature for subsequent operations;

[0027] S8, centrifugal filtration: transfer the raw materials treated above into a centrifuge; set the centrifuge speed to 500-10000r / min, and the centrifugation time to 10-15 minutes;

[0028] Under the action of high-speed centrifugal force, the fibers and macromolecular substances that cannot be enzymatically hydrolyzed settle to the bottom of the centrifuge tube due to density differences, and the supernatant is the liquid rich in giant salamander liver peptides; carefully collect the supernatant to complete the preparation of giant salamander liver peptide liquid.

[0029] The beneficial effects of the present invention are as follows:

[0030] 1. The formula of the giant salamander liver peptide composition described in the present invention is obtained by extracting giant salamander liver peptide liquid from the body of giant salamander. The ratio of giant salamander protein and amino acid composition is basically the same as that of humans, which is the fundamental reason why it has super absorbency. When the human body ingests the giant salamander liver peptide composition of the present invention, the giant salamander liver peptide liquid can quickly pass through the complex digestive system barrier of the human body and quickly enter the blood circulation. Compared with traditional alcohol sobering and liver protecting products, there is no need for a long digestion and decomposition process, which greatly improves the absorption efficiency. For example, in a comparative experiment, the concentration of effective ingredients in the blood reached a peak 2 hours after taking the traditional product, while the product of the present invention can achieve this effect within 30 minutes, laying a solid foundation for rapid alcohol sobering and liver protection;

[0031] The rapidly absorbed giant salamander liver peptide liquid can quickly act on the key links of alcohol metabolism. On the one hand, it directly participates in the synthesis and activation of alcohol metabolic enzymes in the liver, accelerates the decomposition of alcohol into acetaldehyde, and further converts acetaldehyde into harmless acetic acid for excretion from the body, thereby significantly shortening the residence time of alcohol in the body and quickly relieving uncomfortable symptoms such as dizziness, headache, and nausea after drinking. On the other hand, the giant salamander liver peptide liquid can penetrate deep into damaged liver cells, provide precise nutritional support for cells, stimulate the self-repair mechanism of liver cells, promote liver cell regeneration, reduce alcohol damage to the liver from the root, and achieve efficient alcohol sobering and liver protection effects.

[0032] 2. The giant salamander liver peptide composition formula described in the present invention is rich in lecithin. Lecithin is like a "scavenger" of serum lipids. It can combine with lipid molecules such as cholesterol and triglycerides in the blood, and wrap them to form lipoprotein particles that are easy to transport and metabolize. In this way, lecithin effectively reduces the concentration of cholesterol and triglycerides in the serum, maintains the normal level of blood lipids, and reduces the risk of cardiovascular diseases caused by dyslipidemia;

[0033] In the liver, lecithin plays a vital protective role. It can repair damaged liver cell membrane structures, enhance the stability and fluidity of cell membranes, and prevent further damage to liver cells by harmful substances such as alcohol and toxins. At the same time, lecithin is also involved in the metabolic process of fat in the liver, promoting the transport and decomposition of fat, avoiding excessive accumulation of fat in the liver, effectively preventing the formation of alcoholic fatty liver, and protecting liver health in all aspects;

[0034] Lecithin is also of great significance to brain health. It is an important component of nerve cell membranes, can provide sufficient nutrition for nerve cells, promote the synthesis and transmission of neurotransmitters, and thus improve memory, attention and cognitive ability. In terms of enhancing immunity, lecithin can regulate the activity of immune cells, promote the synthesis of immunoglobulins, enhance the body's immune defense capabilities, and make the body more capable of resisting the invasion of external pathogens, especially when the body's immunity decreases after drinking, it plays a key protective role.

[0035] 3. The giant salamander liver peptide composition formula described in the present invention, Pueraria root as an important component of the composition of the present invention, can significantly enhance the activity of acetaldehyde reductase in the liver. Acetaldehyde reductase is a key enzyme in the alcohol metabolism process. The increase in its activity means that after alcohol is converted into acetaldehyde, it can be reduced to acetic acid more quickly, accelerating the metabolism of alcohol in the body. Studies have shown that after taking the composition of the present invention containing Pueraria root, the metabolism time of alcohol in the body is shortened by about 30% compared to when not taking it, greatly reducing the time of alcohol damage to various organs of the body, especially the liver;

[0036] Synergy and absorption promotion: There is a wonderful synergy between Pueraria root and giant salamander liver peptide liquid. Pueraria root can not only accelerate alcohol metabolism itself, but also create more favorable conditions for the absorption and function of giant salamander liver peptide liquid. It can improve the blood circulation of the liver, increase the uptake and utilization efficiency of giant salamander liver peptide liquid by liver cells, so that the effects of the two in sobering up and protecting the liver are superimposed and amplified. At the same time, certain components in Pueraria root can also promote the intestinal absorption of other nutrients, further improve the overall absorbability of the giant salamander liver peptide composition of the present invention by the human body, and achieve a comprehensive improvement from promoting absorption to enhancing efficacy. DETAILED DESCRIPTION

[0037] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods.

[0038] A formula of a giant salamander liver peptide composition according to an embodiment of the present invention is characterized in that the formula is composed of the following raw materials in proportion by weight:

[0039] 10 to 20 portions of giant salamander liver peptide liquid;

[0040] 50 to 150 parts of ginseng;

[0041] 20 to 40 copies of snow lotus culture;

[0042] Yeast β-glucan 1 to 5 parts;

[0043] 30 to 60 parts of Pueraria root;

[0044] 40 to 60 parts of mulberry leaves;

[0045] 35 to 60 servings of bitter melon;

[0046] 40 to 60 parts of Poria cocos;

[0047] 20 to 40 parts of honey.

[0048] A preparation process of a giant salamander liver peptide composition formula, the preparation process comprising the following steps:

[0049] S1. Raw material preparation: Select fresh and healthy liver tissue of artificially cultured giant salamander as the starting material, and ensure that the raw material is free of lesions and pollution;

[0050] S2. Tissue pounding: Put the selected liver tissue of giant salamander into a tissue pounder, and beat it into a fine and uniform meat paste by a high-speed rotating blade for subsequent processing;

[0051] S3, high temperature and high pressure aging: transfer the beaten minced meat to the high temperature and high pressure equipment; set the equipment parameters, and perform aging treatment on the protein tissue in the minced meat for 10 minutes under a high temperature of 121°C and a corresponding high pressure environment; this process can moderately denature the protein structure, which is more conducive to the subsequent enzymatic hydrolysis reaction;

[0052] S4. Material cooling: After the aging treatment is completed, the material is taken out from the high temperature and high pressure equipment; a combination of air cooling and water cooling can be used to quickly reduce the material temperature to 50°C and keep the temperature stable to create suitable conditions for the enzymatic reaction;

[0053] S5, enzymatic hydrolysis reaction: adding compound enzyme to the material cooled to 50°C according to the precise ratio of material to liquid of 1:4; during the enzymatic hydrolysis process, maintaining the temperature of the reaction system at a constant 50°C and maintaining the natural pH value without additional acid-base adjustment;

[0054] Based on the quality of fresh meat, the enzyme addition amount is strictly controlled at 2000-4000U / g to ensure that the content of compound enzyme in the entire reaction system is stably maintained at 0.1%-0.15%. The enzymatic reaction is continued for 4-8 hours, during which the compound enzyme fully acts to gradually degrade the large molecular protein into small molecular peptide substances;

[0055] S6, enzyme inactivation treatment: After the enzymatic reaction is completed, in order to prevent the enzyme from continuing to act and causing excessive enzymatic hydrolysis, the material is quickly placed in boiling water at 100°C for 15 minutes to completely terminate the enzyme activity;

[0056] S7, cooling to room temperature: After the enzyme is inactivated, the material is cooled naturally or by appropriate cooling measures to reduce its temperature to room temperature for subsequent operations;

[0057] S8, centrifugal filtration: transfer the raw materials treated above into a centrifuge; set the centrifuge speed to 500-10000r / min, and the centrifugation time to 10-15 minutes;

[0058] Under the action of high-speed centrifugal force, the fibers and macromolecular substances that cannot be enzymatically hydrolyzed settle to the bottom of the centrifuge tube due to density differences, and the supernatant is the liquid rich in giant salamander liver peptides; carefully collect the supernatant to complete the preparation of giant salamander liver peptide liquid.

[0059] Embodiment 1

[0060] 1. Formula of Giant Salamander Liver Peptide Composition

[0061] Giant salamander liver peptide liquid: 15 portions. This giant salamander liver peptide liquid has a unique source and a sophisticated preparation process, and is the key ingredient for subsequent efficacy.

[0062] Ginseng: 100 servings. Select high-quality ginseng from Changbai Mountain. The unique geographical environment and climatic conditions in this area make ginseng have a long growth cycle and high content of effective ingredients. Ginseng is rich in various ginsenosides, such as ginsenoside Rg1, Rb1, etc. These ingredients have the effects of regulating body functions and enhancing immunity. In this composition, they work together with other ingredients to play a role in sobering up and protecting the liver. It should be noted that according to relevant regulations, the daily consumption of ginseng per person should not exceed 3g to ensure safe and effective intake.

[0063] Snow lotus culture: 30 servings. Obtained through advanced plant cell culture technology, under strictly controlled culture conditions, its active ingredients are stable and high in content. Snow lotus culture contains a variety of biologically active ingredients such as flavonoids and polysaccharides, which have antioxidant and anti-inflammatory effects and are of positive significance for liver protection. The daily consumption per person should not exceed 4g.

[0064] Yeast β-glucan: 3 servings. It is extracted from a specific yeast strain using a patented extraction process, with a purity of more than 98%. Yeast β-glucan has a unique triple helix structure that can enhance the activity of immune cells and improve immunity, while also repairing liver damage caused by alcohol. The daily intake per person should not exceed 250 mg.

[0065] Pueraria root: 42 servings. Pueraria root is carefully selected and grown in pollution-free mountainous areas in Hubei and other places. It is rich in puerarin. Puerarin can accelerate alcohol metabolism and reduce direct damage to the liver caused by alcohol. It also has the effect of dilating blood vessels and improving microcirculation, which helps to sober up and protect the liver.

[0066] Mulberry leaves: 50 servings. Use fresh mulberry leaves picked in spring, when the content of active ingredients such as flavonoids and polysaccharides in mulberry leaves is the highest. Mulberry leaves have the effects of clearing the liver and improving eyesight, dispersing wind and heat, effectively reducing the burden on the liver, and have a certain protective effect on alcoholic liver damage.

[0067] Bitter melon: 48 servings. Organically grown bitter melons are used to ensure that there is no pesticide residue. Bitter melons contain a variety of bioactive substances such as momordica glycosides and flavonoids, which have antioxidant and lipid-lowering effects, help maintain the normal metabolic function of the liver, and assist in sobering up and protecting the liver.

[0068] Poria: 52 servings. Select high-quality Poria from Yunnan and other places, and process it through strict processing technology. Poria is rich in ingredients such as Poria polysaccharide, which has the effects of promoting water and dampness, strengthening the spleen and calming the mind, and has a positive effect on improving liver function and promoting body metabolism.

[0069] Honey: 28 portions. Made of natural mature acacia honey, the degree of Baume is above 42 degrees. Honey is rich in glucose, fructose, vitamins and minerals, which can replenish energy for the body and have a certain detoxification effect, helping to relieve the stimulation of alcohol on the body.

[0070] 2. Preparation process of giant salamander liver peptide liquid

[0071] S1. Tissue crushing and maturation: The liver tissue of artificially cultivated giant salamanders is used as raw material. These giant salamanders grow in a standardized breeding environment to ensure that the liver is healthy and pollution-free. Use a professional tissue crusher to beat the giant salamander liver tissue into a fine and uniform minced meat in preparation for subsequent processing. Add strictly purified water at a material-liquid ratio of 1:4, and then place the mixed material in a high-temperature and high-pressure cooking equipment, and cook it for 10 minutes at a high temperature of 121°C and a corresponding high-pressure environment for maturation. This process can not only cause moderate changes in the protein structure in the giant salamander liver tissue, which is beneficial to subsequent enzymatic hydrolysis, but also play a certain bactericidal role to ensure product safety.

[0072] S2. Enzymatic hydrolysis and enzyme inactivation: The cooked material is quickly cooled to 50°C by a combination of air cooling and water cooling, and the temperature is maintained stable. Under natural pH conditions, the compound enzyme is accurately added in an amount of 3000U / g based on the mass of fresh meat, and the content of the compound enzyme in the reaction system is 0.1%. The compound enzyme is composed of a variety of enzymes with different enzymatic hydrolysis functions, which can work synergistically to efficiently decompose the protein in the liver tissue of giant salamander into small molecule peptide substances. The enzymatic hydrolysis reaction is continued for 6 hours, during which the temperature is kept constant by precise temperature control equipment. After the enzymatic hydrolysis reaction is completed, the material is quickly placed in boiling water at 100°C and inactivated for 15 minutes to completely terminate the activity of the enzyme and prevent excessive enzymatic hydrolysis. After the enzyme inactivation is completed, the material is naturally cooled to room temperature.

[0073] S3, centrifugation and oil removal: transfer the raw material obtained in step (2) to a high-speed centrifuge, set the centrifuge speed to 8000r / min, and centrifuge for 12 minutes. Under the strong centrifugal force, the non-enzymatically degradable fibers, macromolecular impurities, and grease in the material are separated into layers due to density differences. Use professional oil removal equipment to carefully remove the grease to ensure the purity and quality of the giant salamander liver peptide liquid. Finally, carefully collect the supernatant to obtain a high-purity giant salamander liver peptide liquid.

[0074] Embodiment 2

[0075] 1. Formula of Giant Salamander Liver Peptide Composition

[0076] Giant salamander liver peptide liquid: 10 portions. Its preparation process also follows strict standards to ensure stable quality.

[0077] Ginseng: 150 servings. The ginseng selected this time is from Fusong, Jilin, where the soil is fertile and the ginseng is of excellent quality. Ginseng contains a rich variety of ginsenosides and a high content, which provides a strong guarantee for the efficacy of the product.

[0078] Snow lotus culture: 20 servings. Obtained through optimized plant cell culture technology, precise control of factors such as light, temperature, and nutrients during the culture process ensures uniform quality and stable active ingredients of snow lotus culture.

[0079] Yeast β-glucan: 1 part. It is extracted from high-quality yeast using advanced separation and purification technology and has good biological activity and solubility.

[0080] Pueraria root: 60 servings. Pueraria root grown in the mountainous area of ​​Sichuan is selected. The unique local climate and soil conditions make the Pueraria root of high quality and rich in active ingredients such as puerarin.

[0081] Mulberry leaves: 40 servings. Use mulberry leaves picked before the frost in autumn. At this time, the mulberry leaves have fully grown and are rich in nutrients, with high content of active substances such as flavonoids and polysaccharides.

[0082] Bitter melon: 35 servings. Bitter melon is grown using green planting technology, and the use of pesticides is strictly controlled to ensure the natural purity of the bitter melon.

[0083] Poria: 60 parts. Poria from Anhui and other places is selected, and the traditional and modern processing technology is used to retain its effective ingredients and enhance its medicinal value.

[0084] Honey: 40 servings. Made from natural mature vitex honey, it is transparent in color, fragrant in taste, and rich in a variety of nutrients that are beneficial to the human body.

[0085] 2. Preparation process of giant salamander liver peptide liquid

[0086] S1. Tissue crushing and maturation: Use the liver tissue of artificially cultivated giant salamander as the starting material to ensure that the raw material is fresh and healthy. Use a tissue crusher to fully beat the liver tissue of the giant salamander into minced meat. Add strictly purified water in a 1:4 material-liquid ratio, and then place the mixture in a high-temperature and high-pressure equipment, and cook it at a high temperature of 121°C and the corresponding high pressure for 10 minutes to complete the maturation process. This process changes the protein structure in the liver tissue of the giant salamander, which is beneficial to subsequent enzymatic hydrolysis and kills harmful microorganisms at the same time.

[0087] S2. Enzymatic hydrolysis and enzyme inactivation: The cooked material is cooled to 50°C. Under natural pH conditions, the compound enzyme addition amount is 2000U / g based on the mass of fresh meat, and the compound enzyme content is 0.15%. The compound enzyme can specifically hydrolyze the protein in the liver tissue of giant salamander, and the reaction lasts for 4 hours. After the enzymatic hydrolysis is completed, the material is quickly placed in 100°C boiling water to inactivate the enzyme for 15 minutes, and then naturally cooled to room temperature.

[0088] S3, centrifugation and oil removal: put the raw material obtained in step (2) into a centrifuge, set the speed to 5000r / min, and centrifuge for 15 minutes. Use centrifugal force to separate the material into layers, use professional equipment to remove the oil, and finally collect the supernatant to obtain the giant salamander liver peptide liquid.

[0089] Embodiment 3

[0090] 1. Formula of Giant Salamander Liver Peptide Composition

[0091] Giant salamander liver peptide liquid: 20 portions. The preparation of giant salamander liver peptide liquid has gone through many strict processes to ensure its quality and activity.

[0092] Ginseng: 50 servings. Ginseng from Huanren, Liaoning Province is used, which is famous for its excellent quality and high content of active ingredients.

[0093] Snow lotus culture: 40 copies. Through advanced biological culture technology, snow lotus cells are cultivated in a sterile and controlled environment to obtain high-quality snow lotus culture.

[0094] Yeast β-glucan: 5 parts. Yeast β-glucan extracted by patented technology has the characteristics of high purity and high activity.

[0095] Pueraria root: 30 parts. Pueraria root grown in Shaanxi and other places is selected. The local natural environment provides good conditions for the growth of Pueraria root, and the quality of Pueraria root is excellent.

[0096] Mulberry leaves: 60 servings. Mulberry leaves picked during the spring growth season are selected. At this time, the mulberry leaves are fresh, nutritious, and have a high content of active ingredients.

[0097] Bitter melon: 60 servings. Bitter melon grown using organic cultivation techniques, with no pesticide residue, ensures the natural purity and nutritional value of the bitter melon.

[0098] Poria: 40 parts. Poria from Hunan and other places is selected and processed strictly to ensure its quality and medicinal value.

[0099] Honey: 20 servings. Made from natural, mature rapeseed honey, rich in vitamins, minerals, enzymes and other nutrients.

[0100] 2. Preparation process of giant salamander liver peptide liquid

[0101] S1. Tissue crushing and maturation: Select healthy liver tissue of artificially cultivated giant salamanders and use a tissue crusher to beat it into uniform minced meat. Add purified water at a material-liquid ratio of 1:4, put it into a high-temperature and high-pressure equipment, and cook it at 121℃ for 10 minutes to achieve maturation, change the protein structure and sterilize it.

[0102] S2. Enzyme hydrolysis and enzyme inactivation: The cooked material was cooled to 50°C, and at natural pH, the compound enzyme was added at a dosage of 4000U / g based on the mass of fresh meat, and the compound enzyme content was 0.12%. The enzymatic hydrolysis reaction was carried out for 4 hours, and after the enzymatic hydrolysis was completed, the enzyme was rapidly inactivated with 100°C boiling water for 15 minutes, and then cooled to room temperature.

[0103] S3, centrifugation and oil removal: put the raw materials in step (2) into a centrifuge, set the speed to 10000r / min, and centrifuge for 10 minutes. Centrifuge to separate the materials, remove the oil, and collect the supernatant to obtain the giant salamander liver peptide liquid.

[0104] Embodiment 4

[0105] 1. Formula of Giant Salamander Liver Peptide Composition

[0106] Giant salamander liver peptide liquid: 13 parts. Its preparation process pays attention to details to ensure the quality and activity of the peptide liquid.

[0107] Ginseng: 72 parts. Ginseng from Heilongjiang region is used, as the ginseng growing environment in this region is excellent and the quality of ginseng is guaranteed.

[0108] Snow lotus culture: 42 parts. By optimizing the culture conditions, high-quality snow lotus culture is obtained, which has a high content of active ingredients.

[0109] Yeast β-glucan: 1.7 parts. High-purity β-glucan is extracted from yeast using advanced extraction and purification technology.

[0110] Pueraria root: 38 parts. Pueraria root grown in Henan and other places is selected. The local climate and soil conditions are suitable for the growth of Pueraria root, and the quality of Pueraria root is good.

[0111] Mulberry leaves: 46 servings. Use mulberry leaves picked in summer, which are rich in nutrients and have a moderate content of active substances.

[0112] Bitter melon: 37 servings. Bitter melon is grown using ecological planting technology to ensure the quality and nutritional value of the bitter melon.

[0113] Poria cocos: 41 parts. Poria cocos from Guangxi and other places are selected and strictly tested for quality and processed.

[0114] Honey: 36 servings. Made from natural and mature jujube honey, it has a mellow taste and rich nutrition.

[0115] 2. Preparation process of giant salamander liver peptide liquid

[0116] S1. Tissue crushing and maturation: The liver tissue of artificially cultivated giant salamander is used as raw material, and is beaten into minced meat with a tissue crusher. Purified water is added at a material-liquid ratio of 1:4, and the mixture is cooked at 121℃ for 10 minutes for maturation to create conditions for enzymatic hydrolysis and ensure product safety.

[0117] S2. Enzyme hydrolysis and enzyme inactivation: The cooked material was cooled to 50°C, and at natural pH, the compound enzyme was added at 3000U / g based on the mass of fresh meat, and the compound enzyme content was 0.14%. The enzymatic hydrolysis reaction was carried out for 6.5 hours. After the enzymatic hydrolysis was completed, the enzyme was inactivated with 100°C boiling water for 15 minutes, and then cooled to room temperature.

[0118] S3, centrifugation and oil removal: put the raw material obtained in step (2) into a centrifuge, set the speed to 6000r / min, and centrifuge for 13 minutes. After removing the oil, collect the supernatant to obtain the giant salamander liver peptide liquid.

[0119] In summary, the giant salamander liver peptide composition of the present invention is scientifically prepared by a variety of carefully selected raw materials. The giant salamander liver peptide liquid is derived from the giant salamander liver, and its protein and amino acid composition ratio is basically the same as that of humans, and it is easily absorbed by the human body, which greatly improves the absorption and utilization rate of the product of the present invention, so that the effect of sobering up and protecting the liver can be achieved more quickly and effectively during consumption. At the same time, the composition is rich in lecithin, which has the effect of regulating serum lipid levels, not only can reduce cholesterol levels and protect the liver, but also can improve memory, strengthen immunity and enhance the vitality of anti-fatty liver, further strengthening the effect of protecting the liver. Through the diversified formulas and preparation process adjustments of different embodiments, the needs of different groups of people for products can be met, and consumers can be provided with a better quality and more personalized sobering up and protecting liver product selection.

[0120] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A formula of a giant salamander liver peptide composition, characterized in that: The formula consists of the following raw materials in proportion by weight: 10 to 20 portions of giant salamander liver peptide liquid; 50 to 150 portions of ginseng; 20 to 40 copies of snow lotus culture; Yeast β-glucan 1 to 5 parts; 30 to 60 parts of Pueraria root; 40 to 60 parts of mulberry leaves; 35 to 60 servings of bitter melon; 40 to 60 parts of Poria cocos; 20 to 40 parts of honey.

2. A preparation process of a giant salamander liver peptide composition formula, characterized in that: It is used to prepare a giant salamander liver peptide composition formula as claimed in claim 1, and the preparation process comprises the following steps: S1. Raw material preparation: Select fresh and healthy liver tissue of artificially cultured giant salamander as the starting material, and ensure that the raw material is free of lesions and pollution; S2. Tissue pounding: Put the selected liver tissue of giant salamander into a tissue pounder, and beat it into a fine and uniform meat paste by a high-speed rotating blade for subsequent processing; S3, high temperature and high pressure aging: transfer the beaten minced meat to the high temperature and high pressure equipment; set the equipment parameters, and perform aging treatment on the protein tissue in the minced meat for 10 minutes under a high temperature of 121°C and a corresponding high pressure environment; this process can moderately denature the protein structure, which is more conducive to the subsequent enzymatic hydrolysis reaction; S4. Material cooling: After the aging treatment is completed, the material is taken out from the high temperature and high pressure equipment; a combination of air cooling and water cooling can be used to quickly reduce the material temperature to 50°C and keep the temperature stable to create suitable conditions for the enzymatic reaction; S5, enzymatic hydrolysis reaction: adding compound enzyme to the material cooled to 50°C according to the precise ratio of material to liquid of 1:4; during the enzymatic hydrolysis process, maintaining the temperature of the reaction system at a constant 50°C and maintaining the natural pH value without additional acid-base adjustment; Based on the quality of fresh meat, the enzyme addition amount is strictly controlled at 2000-4000U / g to ensure that the content of compound enzyme in the entire reaction system is stably maintained at 0.1%-0.15%. The enzymatic reaction is continued for 4-8 hours, during which the compound enzyme fully acts to gradually degrade the large molecular protein into small molecular peptide substances; S6. Enzyme inactivation treatment: After the enzymatic reaction is completed, in order to prevent the enzyme from continuing to act and causing excessive enzymatic hydrolysis, the material is quickly placed in boiling water at 100°C for 15 minutes to completely terminate the enzyme activity; S7, cooling to room temperature: After the enzyme is inactivated, the material is cooled naturally or by appropriate cooling measures to reduce its temperature to room temperature for subsequent operations; S8, centrifugal filtration: transfer the processed raw materials into a centrifuge; set the centrifuge speed to 500-10000r / min, and the centrifugation time to 10-15 minutes; Under the action of high-speed centrifugal force, the fibers and macromolecular substances that cannot be enzymatically hydrolyzed settle to the bottom of the centrifuge tube due to density differences, and the supernatant is the liquid rich in giant salamander liver peptides; carefully collect the supernatant to complete the preparation of giant salamander liver peptide liquid.