Facial mask liquid, preparation method and application

Abstract

The invention discloses a mask liquid, a preparation method and application, the mask liquid is prepared from the following raw materials by weight: 3 to 15% of a ribonucleic acid composition, 1 to 5% of butylene glycol, 1 to 4% of glycerol dioleate, 0.2 to 0.6% of panthenol, 0.1 to 0.5% of 1, 2-hexanediol, 0.25 to 0.65% of p-hydroxyacetophenone, 0.2 to 0.5% of xanthan gum, 0.05 to 0.15% of dipotassium glycyrrhizinate, 0.01 to 0.06% of hydrolyzed sodium hyaluronate, 0.02 to 0.05% of sodium hyaluronate, 0.01 to 0.045% of a thickening agent, and the balance of water. And the balance of deionized water. Ribonucleic acid in the ribonucleic acid composition is obtained through biosynthesis, the flexible liposome assists the ribonucleic acid in permeating skin epidermis to directly reach keratinocytes or skin fibroblasts, and experiments prove that the mask liquid has good effects of improving skin sensitivity and tightening skin.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a facial mask liquid, a preparation method and application thereof. Background Art

[0002] The skin is the largest organ in the human body. It not only protects the body from external environmental damage, but also has functions such as sensation, temperature regulation and excretion. The skin is composed of three layers: epidermis, dermis and subcutaneous tissue. The epidermis is responsible for protecting the skin from external substances, and the dermis contains elastic fibers and collagen fibers, which play a key role in the elasticity and firmness of the skin. As we age, the skin will gradually show aging phenomena, such as wrinkles, sagging, and spots. These phenomena are partly caused by factors such as slowed skin cell renewal, reduced collagen and free radical damage.

[0003] Keratinocytes are the main cell type in the epidermis, accounting for more than 80% of the total number of epidermal cells. They have important functions such as shielding, protecting the body from external physical, chemical and biological factors, regeneration and repair, keratinization process, pigmentation and immune response, and play a vital role in the health and functional integrity of the skin. Skin fibroblasts belong to dermal cells and have functions such as producing collagen and elastin, wound healing and maintaining the overall structure and integrity of the skin. They play an important role in the skin repair and regeneration process.

[0004] Facial masks are a common skin care product in daily life. They use a short stay on the face to promote skin metabolism and achieve a facial skin care effect. Although the prior art provides a variety of facial masks containing different types of RNA or DNA that have certain effects of promoting skin cell regeneration, anti-aging, anti-allergy and soothing, the actual effect is not ideal. Therefore, there is an urgent need for a facial mask liquid that can truly enhance cell function and effectively promote skin anti-allergy and soothing and anti-aging. Summary of the invention

[0005] In order to overcome the deficiencies of the prior art, the present invention provides a facial mask liquid, a preparation method and an application. The facial mask liquid has good skin permeability, can enable RNA to directly reach the keratinocytes and skin fibroblasts of the skin, play a specific targeting effect at the gene level, and then directly regulate the physiological state of keratinocytes and skin fibroblasts, has good effects in anti-skin aging and skin anti-allergy and soothing, and can effectively solve the deficiencies of the prior art.

[0006] The technical solution provided by the present invention is as follows:

[0007] The first aspect of the present invention provides a facial mask liquid, which is made of the following raw materials in percentage by weight:

[0008] RNA composition 3-15, butylene glycol 1-5, glyceryl dioleate 1-4, panthenol 0.2-0.6, 1,2-hexanediol 0.1-0.5, p-hydroxyacetophenone 0.25-0.65, xanthan gum 0.2-0.5, dipotassium glycyrrhizate 0.05-0.15, hydrolyzed sodium hyaluronate 0.01-0.06, sodium hyaluronate 0.02-0.05, thickener 0.01-0.045, and the balance is deionized water.

[0009] Furthermore, the RNA composition is composed of the following components in weight percentage: 0.002-0.003 of RNA, 0.01-0.015 of diolein, 0.003-0.0045 of hydrogenated lecithin, 0.002-0.003 of polyethyleneimine-10, 0.0022-0.0033 of cholesterol, and the balance is deionized water.

[0010] Furthermore, the ribonucleic acid comprises a tRNA scaffold and a recombinant functional RNA having a miR-34a precursor sequence.

[0011] Furthermore, the recombinant functional RNA is prepared by transforming HST08 competent cells with a recombinant functional RNA expression plasmid.

[0012] Furthermore, the thickener is one or more of hydroxyethyl cellulose, disodium hydrogen phosphate, polysorbate 60, and sodium dihydrogen phosphate.

[0013] Furthermore, the effective target cells of the facial mask liquid are skin fibroblasts and keratinocytes.

[0014] Furthermore, the preparation process of the ribonucleic acid is as follows:

[0015] Constructing a recombinant functional RNA expression plasmid, wherein the recombinant functional RNA expression plasmid includes a recombinant functional RNA sequence including a tRNA scaffold, a miR-34a precursor sequence, and a small RNA sequence with anti-aging and anti-allergic soothing functions;

[0016] HST08 competent cells were transformed by recombinant functional RNA expression plasmids, the transformed HST08 competent cells were added to LB medium for culture, the bacterial sludge was collected and resuspended in magnesium acetate-Tris·HCl solution, saturated phenol was added, the aqueous phase was collected, NaCl solution was added to precipitate macromolecular impurities, the supernatant was collected, anhydrous ethanol was added to the supernatant, and total RNA was obtained after centrifugation;

[0017] Separating and purifying the total RNA using a strong anion exchange column in FPLC to obtain recombinant functional RNA;

[0018] The recombinant functional RNA is treated to remove endotoxin to obtain the ribonucleic acid required for the mask liquid.

[0019] Furthermore, the preparation process of the RNA composition is as follows:

[0020] Weigh glycerol dioleate, hydrogenated lecithin, polyethyleneimine-10, and cholesterol according to weight percentage, add solvent ethanol, and perform rotary evaporation to form a film;

[0021] The liquid was transferred to a centrifuge tube and mechanically sonicated under ice bath conditions to obtain flexible liposomes;

[0022] Under sterile conditions, RNA was dissolved in sterile endotoxin-free deionized water;

[0023] Under sterile conditions, the RNA aqueous solution and the flexible liposomes are stirred and mixed in a certain ratio to obtain a composition;

[0024] Quality standards of the RNA composition: appearance is colorless or milky white liquid; pH: 5-8; total colony count (CFU / g): <10; total mold and yeast count (CFU / g): <10; pathogenic bacteria / g (heat-resistant coliform group, Pseudomonas aeruginosa, Staphylococcus aureus): must not be detected; methanol: <2000 mg / kg, not detected; lead: <10 mg / kg, not detected; mercury: <1 mg / kg, not detected; arsenic: <2 mg / kg, not detected; cadmium: <5 mg / kg, not detected.

[0025] The second aspect of the present invention also provides a method for preparing the facial mask liquid as described above, comprising the following steps:

[0026] Obtaining a RNA composition and storing it at 4-25°C;

[0027] Butylene glycol, glyceryl dioleate, panthenol, 1,2-hexanediol, p-hydroxyacetophenone, xanthan gum, dipotassium glycyrrhizinate, hydrolyzed sodium hyaluronate, sodium hyaluronate, hydroxyethyl cellulose, disodium hydrogen phosphate, polysorbate 60, sodium dihydrogen phosphate and deionized water are added in a formula ratio to the composition containing ribonucleic acid to 100%, and mixed evenly to obtain an anti-allergic, soothing and anti-aging facial mask liquid containing ribonucleic acid.

[0028] The third aspect of the present invention provides a use of the facial mask liquid prepared by the preparation method as described above or as described above in creams, essences, stock solutions, essence water, eye creams and body lotions.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The present invention provides a facial mask liquid with anti-allergic, soothing and anti-aging functions containing RNA, which can promote skin cell metabolism, increase the content of skin collagen and elastin, promote the shedding of old and damaged stratum corneum and enhance the formation of new stratum corneum, reshape the skin barrier, and thus effectively improve skin aging and skin sensitivity problems. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a VSIA facial deep and shallow red area and UV detection image of one of the facial mask liquids prepared by the present invention after 28 days of use;

[0032] Figure 2 This is another example of the facial mask liquid prepared by the present invention after 28 days of use, and the VSIA facial deep and shallow red areas and ultraviolet detection images;

[0033] Figure 3 This is the VSIA facial deep and shallow red area and UV detection image of the third example of the facial mask liquid prepared by the present invention after 28 days of use.

[0034] Figure 4 This is the VSIA facial deep and shallow red area and UV detection image of the third example of the facial mask liquid prepared by the present invention after 28 days of use. DETAILED DESCRIPTION

[0035] In order to make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the embodiments described below are part of the embodiments of the present application, rather than all of the embodiments. The components of the embodiments of the present application described and shown in the drawings here can be arranged and designed in various different configurations.

[0036] In addition, the indefinite articles "a" and "an" before the elements or components of the present invention have no restrictions on the quantity requirements (i.e. the number of occurrences) of the elements or components. Therefore, "a" or "an" should be interpreted as including one or at least one, and the elements or components in the singular form also include the plural form, unless the number is obviously only referred to in the singular form.

[0037] Example 1: RNA Accelerated Stabilization Experiment

[0038] RNA has poor stability and is easily degraded by various external factors. Therefore, stability is crucial to whether the RNA in the mask liquid can play an effective role. This example uses an RNA accelerated stability experiment to test the stability of RNA.

[0039] The RNA was placed in neutral, acidic and cosmetic matrix conditions, and the degradation of RNA was tested at different temperatures (25°C, 45°C). By applying the principles and methods of chemical kinetics, the reaction rate of the drug at different temperatures was measured by the accelerated experimental method. According to the Arrhenius formula, the stability period of RNA at room temperature was calculated.

[0040]

[0041]

[0042] Wherein, C0 represents the concentration of RNA in the liquid preparation at t=0, C t is the RNA concentration of the liquid preparation at time t, K is the rate constant, and T is Kelvin.

[0043] (1) Sample treatment: 0.03 μg of RNA was encapsulated with flexible liposomes at a mass ratio of 1:2, and DEPC water was added to prepare a 1 mL liquid preparation, which was placed in a 1.5 mL EP tube for storage. The liquid preparation was prepared according to the experimental requirements (under neutral conditions) according to the above method.

[0044] (2) 0.03 μg of RNA was encapsulated with flexible liposomes at a mass ratio of 1:2, and 95% deionized water and 5% butylene glycol were added to prepare a 1 mL liquid preparation, which was placed in a 1.5 mL EP tube for storage. The liquid preparation (under cosmetic matrix conditions) was prepared according to the experimental requirements according to the above method.

[0045] (3) 0.03 μg of RNA was encapsulated with flexible liposomes at a mass ratio of 1:2 and an acetic acid-sodium acetate buffer solution with a pH of 5.5 was added (acetic acid-sodium acetate buffer solution: weigh 200 g CH3COONa·3H2O and dissolve it in water, add 10 mL glacial acetic acid, dilute with water to 1000 mL, and shake well) to prepare a 1 mL liquid preparation and store it in a 1.5 mL EP tube. The liquid preparation (under acidic conditions) was prepared according to the experimental requirements according to the above method.

[0046] (4) Accelerated condition treatment: The liquid preparations under the above three conditions were divided into three groups, each with 18 samples. Each group of 18 samples was placed in a constant temperature water bath at 25°C and 45°C for accelerated test, and 3 samples were taken out at 0h, 5h and 10h, respectively. The taken liquid preparations were immediately cooled to terminate the reaction.

[0047] (5) Bile acid lysis: After cooling the liquid preparations taken out at different temperatures and for different times, the liposomes are first broken up with 10% bile acid solution to release the RNA. After adding the bile acid solution, the mixture is rapidly shaken for 30 minutes to allow the reaction to complete.

[0048] (6) RNA extraction: RNA was extracted from the samples after the above treatment using an RNA kit (EZNA total RNA Kit I) according to the RNA extraction procedure.

[0049] (7) Concentration measurement: The absorbance of the RNA solution at 260 nm is measured by an ultraviolet spectrophotometer to calculate the RNA content, and the RNA content of the treated sample is obtained and the content percentage is calculated.

[0050] Table 1, Table 2 and Table 3 are the calculated RNA stability periods under neutral conditions, acidic conditions and cosmetic matrix conditions respectively. The results show that RNA has good stability under different conditions. Under 25°C, the stability period of RNA under neutral conditions, acidic conditions and cosmetic matrix conditions can reach more than 900 days; under 45°C, the stability period of RNA under neutral conditions, acidic conditions and cosmetic matrix conditions can reach more than 160 days. The above results show that RNA can exist stably in the mask liquid environment for a long time, laying the foundation for its function.

[0051] Table 1 Neutral conditions

[0052]

[0053] Table 2 Acidic conditions

[0054]

[0055] Table 3 Cosmetic matrix conditions

[0056]

[0057] The present invention provides a facial mask liquid, which is made of the following raw materials in percentage by weight:

[0058] RNA composition 3-15, butylene glycol 1-5, glyceryl dioleate 1-4, panthenol 0.2-0.6, 1,2-hexanediol 0.1-0.5, p-hydroxyacetophenone 0.25-0.65, xanthan gum 0.2-0.5, dipotassium glycyrrhizate 0.05-0.15, hydrolyzed sodium hyaluronate 0.01-0.06, sodium hyaluronate 0.02-0.05, thickener 0.01-0.045, and the balance is deionized water.

[0059] In the above-mentioned facial mask liquid, the RNA composition ratio is: RNA 0.002-0.003%, glyceryl dioleate 0.01-0.015%, hydrogenated lecithin 0.003-0.0045%, polyethyleneimine-10 0.002-0.003%, cholesterol 0.0022-0.0033%, and deionized water to 100%.

[0060] In the above-mentioned facial mask liquid, the RNA preparation process is as follows:

[0061] (1) Construct a recombinant functional RNA expression plasmid or directly use an existing plasmid (which can meet the requirements of the subsequent steps). The recombinant functional RNA expression plasmid contains a recombinant functional RNA sequence including a tRNA scaffold, a miR-34a precursor sequence, and a small RNA sequence with anti-aging and anti-allergic soothing functions.

[0062] (2) The recombinant functional RNA expression plasmid was transformed into HST08 competent cells, and LB medium was added for culture. The bacterial sludge was collected and resuspended in magnesium acetate-Tris·HCl solution. Saturated phenol was then added, the aqueous phase was collected, and NaCl solution was added to precipitate macromolecular impurities. The supernatant was collected, and anhydrous ethanol was added to the supernatant. After centrifugation, total RNA was obtained.

[0063] (3) Separating and purifying the total RNA using a strong anion exchange column in FPLC to obtain recombinant functional RNA.

[0064] (4) The recombinant functional RNA is treated to remove endotoxin to obtain the ribonucleic acid required for the facial mask liquid.

[0065] The production process of RNA is simple, the yield is high, the acquisition is convenient, the cost is low, and it also has good biological activity and stability. It has a good targeted regulatory effect on genes. Therefore, it can specifically regulate the activity of skin cells and achieve the effects of anti-aging, anti-allergic and soothing the skin.

[0066] The preparation process of the RNA composition is as follows:

[0067] (1) Add glycerol dioleate, hydrogenated lecithin, polyethyleneimine-10, cholesterol and solvent ethanol into an eggplant-shaped bottle in proportion, assemble the eggplant-shaped bottle onto a rotary evaporator, turn on the vacuum pump, set the temperature to 40° C., rotate at 80-100 rpm, and perform rotary evaporation to form a film for 25 minutes.

[0068] (2) The liquid in the eggplant-shaped flask is transferred to a centrifuge tube and mechanically sonicated under ice bath conditions to obtain flexible liposomes.

[0069] (3) Under sterile conditions, RNA is dissolved in sterile endotoxin-free deionized water.

[0070] (4) Under sterile conditions, the RNA aqueous solution and the flexible liposomes are stirred and mixed in a certain ratio to obtain a composition.

[0071] Product quality standards of the composition: appearance is colorless or milky white liquid; pH: 5-8; total colony count (CFU / g): <10; total mold and yeast count (CFU / g): <10; pathogenic bacteria / g (heat-resistant coliform group, Pseudomonas aeruginosa, Staphylococcus aureus): not detectable; methanol: <2000mg / kg, not detected; lead: <10mg / kg, not detected; mercury: <1mg / kg, not detected; arsenic: <2mg / kg, not detected; cadmium: <5mg / kg, not detected.

[0072] In step (4), the ratio of RNA to flexible liposome added to the facial mask provided by the present invention is 1:1 to 1:5. The flexible liposome has the function of increasing the RNA stratum corneum permeability, overcoming the problem of low RNA permeability, and solving the problem that RNA is difficult to enter the skin to activate fibroblasts and keratinocytes. The target cells of the facial mask are skin fibroblasts and keratinocytes. By acting on skin fibroblasts and keratinocytes, the facial mask plays an effect in anti-skin aging and anti-skin sensitivity relief.

[0073] The facial mask liquid provided by the invention can be used in creams, essences, stock solutions, essence water, eye creams and body lotions.

[0074] The following embodiments provide the above-mentioned facial mask liquids with several different formulations.

[0075] Example 2

[0076] The present embodiment provides an anti-allergic, soothing and anti-aging facial mask liquid containing RNA, which is composed of the following raw materials in a mass ratio: 5% of a RNA composition with anti-allergic, soothing and anti-aging functions, 2% butylene glycol, 2.5% glycerol dioleate, 0.4% panthenol, 0.4% 1,2-hexanediol, 0.55% p-hydroxyacetophenone, 0.25% xanthan gum, 0.15% dipotassium glycyrrhizate, 0.04% hydrolyzed sodium hyaluronate, 0.04% sodium hyaluronate; 0.04% thickener, deionized water to 100%. The thickener is one or more of hydroxyethyl cellulose, disodium hydrogen phosphate, polysorbate-60, and sodium dihydrogen phosphate.

[0077] Example 3

[0078] The present embodiment provides an anti-allergic, soothing and anti-aging facial mask liquid containing RNA, which is composed of the following raw materials in a mass ratio: 10% of a RNA composition with anti-allergic, soothing and anti-aging functions, 2% butylene glycol, 2.5% glycerol dioleate, 0.4% panthenol, 0.4% 1,2-hexanediol, 0.55% p-hydroxyacetophenone, 0.25% xanthan gum, 0.15% dipotassium glycyrrhizate, 0.04% hydrolyzed sodium hyaluronate, 0.04% sodium hyaluronate; 0.04% thickener, deionized water to 100%. The thickener is one or more of hydroxyethyl cellulose, disodium hydrogen phosphate, polysorbate-60, and sodium dihydrogen phosphate.

[0079] Example 4

[0080] This embodiment provides a method for preparing the above-mentioned facial mask liquid, comprising the following steps:

[0081] (1) obtaining a RNA composition according to the preparation method provided above and storing it at 4-25°C;

[0082] (2) Add butylene glycol, glyceryl dioleate, panthenol, 1,2-hexanediol, p-hydroxyacetophenone, xanthan gum, dipotassium glycyrrhizate, hydrolyzed sodium hyaluronate, sodium hyaluronate, hydroxyethyl cellulose, disodium hydrogen phosphate, polysorbate 60, sodium dihydrogen phosphate, and deionized water in the proportions of Example 2 or 3 to the composition containing ribonucleic acid until the amount reaches 100%, and mix well to obtain an anti-allergic, soothing and anti-aging facial mask liquid containing ribonucleic acid.

[0083] To demonstrate the technical points of the technical solution provided by the present invention, the human body test experimental data of Example 2 and Example 3 of the present invention will be shown below.

[0084] In order to evaluate the effect of the mask solution, several female volunteers were selected for mask testing. After 28 consecutive days, the facial image analysis system was used to detect and evaluate the deep red area, light red area and skin inflammation of the volunteers' facial skin. After an average of 28 consecutive days of mask use, the VISA data results showed that compared with the 0th day (such as Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown in the figure, the inflammation of the deep red area and the shallow red area on the face of the volunteers was significantly improved, and the fading rate was significantly improved. At the same time, the ultraviolet results showed that the facial pigment spots were significantly reduced. The results show that the facial mask liquid provided by the present invention has good anti-allergic, soothing and anti-aging effects.

[0085] The above examples all illustrate that the RNA in the present invention can not only exist stably for a long time in the facial mask liquid, but also has a relatively fast anti-aging and anti-allergic soothing effect.

[0086] The above are all preferred embodiments of the present invention, and are not intended to limit the protection scope of the present application in sequence. Therefore, all equivalent changes made based on the structure, shape, and principle of the present application should be included in the protection scope of the present application.

[0087] The above is only the best specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any changes or substitutions within the technical scope disclosed in the present application shall be included in the protection scope of the present application. Therefore, the protection scope of the present application shall be based on the protection scope of the claims.

Claims

1. A facial mask liquid, characterized in that, The facial mask liquid is made of the following raw materials in percentage by weight: RNA composition 3-15, butylene glycol 1-5, glyceryl dioleate 1-4, panthenol 0.2-0.6, 1,2-hexanediol 0.1-0.5, p-hydroxyacetophenone 0.25-0.65, xanthan gum 0.2-0.5, dipotassium glycyrrhizate 0.05-0.15, hydrolyzed sodium hyaluronate 0.01-0.06, sodium hyaluronate 0.02-0.05, thickener 0.01-0.045, and the balance is deionized water.

2. The facial mask liquid according to claim 1, characterized in that: The ribonucleic acid composition is composed of the following components in weight percentage: 0.002-0.003 ribonucleic acid, 0.01-0.015 glycerol dioleate, 0.003-0.0045 hydrogenated lecithin, 0.002-0.003 polyethyleneimine-10, 0.0022-0.0033 cholesterol, and the balance is deionized water.

3. The facial mask liquid according to claim 2, characterized in that: The ribonucleic acid comprises a tRNA scaffold and a recombinant functional RNA having a miR-34a precursor sequence.

4. The facial mask liquid according to claim 3, characterized in that: The recombinant functional RNA is prepared by transforming HST08 competent cells with a recombinant functional RNA expression plasmid.

5. The facial mask liquid according to any one of claims 1 to 4, characterized in that: The thickener is one or more of hydroxyethyl cellulose, disodium hydrogen phosphate, polysorbate-60, and sodium dihydrogen phosphate.

6. The facial mask liquid according to any one of claims 1 to 4, characterized in that: The effective target cells of the facial mask liquid are skin fibroblasts and keratinocytes.

7. The facial mask liquid according to any one of claims 2 to 4, characterized in that: The preparation process of the ribonucleic acid is as follows: Constructing a recombinant functional RNA expression plasmid, wherein the recombinant functional RNA expression plasmid includes a recombinant functional RNA sequence including a tRNA scaffold, a miR-34a precursor sequence, and a small RNA sequence with anti-aging and anti-allergic soothing functions; HST08 competent cells were transformed by recombinant functional RNA expression plasmids, the transformed HST08 competent cells were added to LB medium for culture, the bacterial sludge was collected and resuspended in magnesium acetate-Tris·HCl solution, saturated phenol was added, the aqueous phase was collected, NaCl solution was added to precipitate macromolecular impurities, the supernatant was collected, anhydrous ethanol was added to the supernatant, and total RNA was obtained after centrifugation; Separating and purifying the total RNA using a strong anion exchange column in FPLC to obtain recombinant functional RNA; The recombinant functional RNA is treated to remove endotoxin to obtain the ribonucleic acid required for the mask liquid.

8. The facial mask liquid according to any one of claims 1 to 4, characterized in that: The preparation process of the RNA composition is as follows: Weigh glycerol dioleate, hydrogenated lecithin, polyethyleneimine-10, and cholesterol according to weight percentage, add solvent ethanol, and perform rotary evaporation to form a film; The liquid was transferred to a centrifuge tube and mechanically sonicated under ice bath conditions to obtain flexible liposomes; Under sterile conditions, RNA was dissolved in sterile endotoxin-free deionized water; Under sterile conditions, the RNA aqueous solution and the flexible liposomes are stirred and mixed in a certain ratio to obtain a composition; Quality standard of the RNA composition: colorless or milky white liquid in appearance; pH: 5-8; Total colony count (CFU / g): <10; Total mold and yeast count (CFU / g): <10; Pathogenic bacteria / g (heat-resistant coliform group, Pseudomonas aeruginosa, Staphylococcus aureus): not detectable; Methanol: <2000mg / kg, not detected; Lead: <10mg / kg, not detected; Mercury: <1mg / kg, not detected; Arsenic: <2mg / kg, not detected; Cadmium: <5mg / kg, not detected.

9. A method for preparing a facial mask liquid as claimed in any one of claims 1 to 4, characterized in that: The following steps are involved: Obtaining a RNA composition and storing it at 4-25°C; Butylene glycol, glyceryl dioleate, panthenol, 1,2-hexanediol, p-hydroxyacetophenone, xanthan gum, dipotassium glycyrrhizinate, hydrolyzed sodium hyaluronate, sodium hyaluronate, hydroxyethyl cellulose, disodium hydrogen phosphate, polysorbate 60, sodium dihydrogen phosphate and deionized water are added in a formula ratio to the composition containing ribonucleic acid to 100%, and mixed evenly to obtain an anti-allergic, soothing and anti-aging facial mask liquid containing ribonucleic acid.

10. Use of the facial mask liquid prepared by the preparation method of the facial mask liquid as claimed in claim 9 or the facial mask liquid as claimed in any one of claims 1 to 4 in creams, essences, stock solutions, essence waters, eye creams and body lotions.

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