Anti-wrinkle face cream

Abstract

The invention relates to the field of cosmetics, in particular to anti-wrinkle face cream. The invention provides an anti-wrinkle face cream which is prepared from the following components in percentage by mass: 0.3%-2.5% of a radix pseudostellariae cyclic peptide solution, 1.0%-5.0% of a cono-spiro peptide solution, 0.1%-5.0% of a pichia pastoris recombinant collagen solution and 1.0%-5.0% of a mixed solution of hydrolyzed collagen and hexapeptide-9. In the technical scheme, the anti-wrinkle face cream has an innovative bicyclic peptide combination; meanwhile, the recombinant double collagen and the marine collagen oligopeptide are compounded, so that the multi-dimensional anti-wrinkle and wrinkle-fading effects are realized, including promoting the generation of collagen, increasing SPARC in a corium layer, increasing the fibrin level in dermis, inhibiting the transmission of skin neural signals and inhibiting the activity of collagenase; therefore, the effect of inhibiting skin aging is effectively improved.

Description

Technical Field

[0001] The present application relates to the field of cosmetics, and in particular to an anti-wrinkle cream. Background Art

[0002] Currently, anti-wrinkle cosmetics usually contain single peptide ingredients such as acetyl peptides and palmitoyl peptides; however, the anti-wrinkle effect of such ordinary peptide cosmetics is single and can no longer meet the market's increasingly high anti-wrinkle demands. Summary of the invention

[0003] The purpose of the embodiments of the present application is to provide an anti-wrinkle cream.

[0004] In a first aspect, the present application provides an anti-wrinkle cream, which comprises, by mass percentage: 0.3%-2.5% of Pseudostellariae cyclic peptide solution, 1.0%-5.0% of conospiropeptide solution, 0.1%-5.0% of Pichia pastoris recombinant collagen solution, and 1.0%-5.0% of a mixed solution of hydrolyzed collagen and hexapeptide-9.

[0005] In the above technical solution, the anti-wrinkle cream innovatively adopts a combination of bicyclic peptides; at the same time, it is compounded with recombinant collagen and marine collagen oligopeptides (hydrolyzed collagen and hexapeptide-9), achieving a multi-dimensional anti-wrinkle and line-lightening effect, including: promoting collagen production, increasing SPARC in the dermis, increasing the level of fibrin in the dermis, inhibiting the transmission of skin nerve signals and inhibiting the activity of collagenase, and promoting collagen production; thereby effectively improving the efficacy of inhibiting skin aging. The various ingredients in the anti-wrinkle cream work together to achieve a synergistic effect; and the various ingredients have high biological activity and good stability, which improves the real anti-wrinkle effect of the cosmetics.

[0006] In other embodiments of the present application, the anti-wrinkle cream includes, by mass percentage: 0.5%-2.0% Pseudomonas aeruginosa cyclic peptide solution, 1.5%-4.0% conospiropeptide solution, 0.5%-4.0% Pichia pastoris recombinant collagen solution, and 1.5%-5.0% mixed solution of hydrolyzed collagen and hexapeptide-9.

[0007] In other embodiments of the present application, the Pseudostellariae Radix cyclopeptide solution comprises: glycerol, water, and Pseudostellariae Radix extract; optionally, in the Pseudostellariae Radix cyclopeptide solution, the mass percentage of the Pseudostellariae Radix extract is 0.1% to 0.3%.

[0008] In other embodiments of the present application, the conocyclic peptide solution includes: water, 1,2-pentanediol, 1,2-hexanediol, and conocyclic peptide; optionally, the mass percentage of the conocyclic peptide in the conocyclic peptide solution is 0.1% to 5%.

[0009] In other embodiments of the present application, the Pichia pastoris recombinant collagen solution includes: water, glycerol, sodium dihydrogen phosphate, sodium dihydrogen phosphate, and soluble collagen. Optionally, in the Pichia pastoris recombinant collagen solution, the mass percentage of the soluble collagen is 0.1% to 5%.

[0010] In other embodiments of the present application, the Pichia pastoris recombinant collagen uses amino acids 1076-1202 of human type I collagen and amino acids 594-846 of type III collagen as templates for human collagen.

[0011] In other embodiments of the present application, the mixture solution of hydrolyzed collagen and hexapeptide-9 includes: water, glycerin, sodium PCA, erythritol, crispy chondrus crispus powder, xanthan gum, hydrolyzed collagen, hexapeptide-9, benzoic acid, and sorbic acid.

[0012] In other embodiments of the present application, the anti-wrinkle cream further comprises: a primary emulsifier; the primary emulsifier is a low melting point C12-20 acid PEG-8 ester.

[0013] In other embodiments of the present application, the anti-wrinkle cream further comprises: avocado butter and cetearyl alcohol.

[0014] In other embodiments of the present application, the anti-wrinkle cream further includes: isononyl isononanoate, polydimethylsiloxane, and ammonium acryloyldimethyltaurate / VP copolymer. DETAILED DESCRIPTION

[0015] In order to make the purpose, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments.

[0016] Therefore, the following detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present application.

[0017] After research, the inventors found that anti-wrinkle cosmetics with peptide ingredients such as acetyl peptides and palmitoyl peptides have a single anti-wrinkle channel, which limits their anti-wrinkle effect. Further research found that the main cause of skin wrinkles is the aging of the dermis structure; and how to protect the dermis structure has become the key to solving anti-wrinkle problems. Further research found that in the dermis, SPARC plays an important role in maintaining the structural integrity of the skin and contributes to multiple processes involved in skin development, maintenance and repair. The level of fibrin I in the dermis affects the maturity and structure of the skin matrix. Further research found that the transmission of skin nerve signals will also affect the dermis structure; and the activity of collagenase will also affect the dermis structure and affect collagen production. Therefore, if you want to improve the dermis structure, you need to improve it from multiple dimensions.

[0018] Based on the above research, an embodiment of the present application provides an anti-wrinkle cream, which includes, by mass percentage: 0.3%-2.5% of Pseudostellaria caerulea cyclic peptide solution, 1.0%-5.0% of conospiropeptide solution, 0.1%-5.0% of Pichia pastoris recombinant collagen solution, and 1.0%-5.0% of a mixed solution of hydrolyzed collagen and hexapeptide-9.

[0019] In the above technical solution, each component cooperates with each other to achieve a synergistic effect. It improves the dermal structure from different dimensions, increases SPARC in the dermis, and increases the synthesis of type I collagen in the dermis; at the same time, it inhibits the transmission of skin nerve signals and the activity of collagenase, and promotes collagen production, thereby achieving multi-dimensional and truly effective protection of the dermal structure and greatly improving the anti-aging effect.

[0020] Furthermore, Pseudostellaria sutchuenensis cyclic peptide: increases the synthesis of type I collagen and improves the structure of the dermis; increases SPARC in the dermis and enhances the maturity and structure of the skin matrix; increases the level of fibrin I in the dermis; conospirocyclic peptide: inhibits the transmission of skin nerve signals; Pichia pastoris recombinant collagen: inhibits the activity of collagenase; a mixture of hydrolyzed collagen and hexapeptide-9: promotes collagen production; the four active ingredients work together from different dimensions to truly improve the structure of the dermis and greatly enhance the anti-aging effect.

[0021] Furthermore, the anti-wrinkle cream of the present application innovatively adopts a bicyclic peptide combination; it also compounds recombinant collagen and marine collagen oligopeptides; each component has high biological activity and good stability, thereby improving the real anti-wrinkle effect of the cosmetic.

[0022] Exemplarily, in some embodiments of the present application, the anti-wrinkle cream includes, by mass percentage: 0.3%, 0.4%, 0.5%, 0.8%, 1%, 1.3%, 1.5%, 1.8%, 2.0%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5% or a range between any two of the foregoing values ​​of a Taizishen cyclic peptide solution; 1.0%, 1.2%, 1.5%, 1.8%, 2.0%, 2.2%, 2.5%, 2.8%, 3.0%, 3.2%, 3.5%, 3.8%, 4.0%, 4.2%, 4.5%, 4.8%, 4.9%, 5.0% or any two of the foregoing conocyclic peptide solution. The invention relates to a range between two values; a recombinant collagen solution of Pichia pastoris 0.1%, 0.2%, 0.3%, 0.5%, 0.8%, 1.0%, 1.2%, 1.5%, 1.8%, 2.0%, 3.0%, 4.0%, 4.5%, 5.0% or a range between any two of the foregoing values; a mixed solution of hydrolyzed collagen and hexapeptide-9 1.0%, 1.2%, 1.5%, 1.8%, 2.0%, 2.2%, 2.5%, 2.8%, 3.0%, 3.2%, 3.5%, 3.8%, 4.0%, 4.2%, 4.5%, 4.8%, 4.9%, 5.0% or a range between any two of the foregoing values.

[0023] Furthermore, in some embodiments of the present application, the anti-wrinkle cream includes, by mass percentage: 0.5%-2.0% Pseudomonas aeruginosa cyclic peptide solution, 1.5%-4.0% conospiropeptide solution, 0.5%-4.0% Pichia pastoris recombinant collagen solution, and 1.5%-5.0% mixed solution of hydrolyzed collagen and hexapeptide-9.

[0024] Exemplarily, in some embodiments of the present application, the anti-wrinkle cream includes, by mass percentage: 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0% or the range between any two of the foregoing values ​​of conocyclic peptide solution; 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 2.9%, 3.0% of conocyclic peptide solution. , 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7%, 3.8%, 3.9%, 4.0% or the range between any two of the foregoing values; Pichia pastoris recombinant collagen solution 0.5%, 0.8%, 1%, 1.2%, 1.5%, 2.0%, 2.5%, 3.0%, 3.5%, 4.0% or the range between any two of the foregoing values; mixed solution of hydrolyzed collagen and hexapeptide-9 1.5%, 1.6%, 1.8%, 2.0%, 2.5%, 3.0%, 3.5%, 4.0%, 4.5%, 5.0% or the range between any two of the foregoing values.

[0025] In the above technical scheme, the Pseudostellariae cyclopeptide compounded in the anti-wrinkle cream is only present in the biologically active cyclopeptide Heterophyllin B of Pseudostellariae radix, which has excellent stability, biological activity and cell permeability, and can effectively promote the skin matrix and regulate epidermal homeostasis, increase collagen synthesis, improve dermal structure, and enhance the maturity and structure of the skin matrix; restart the production of collagen and improve the epidermal structure through retinoid metabolism and appropriate characteristics, exceeding the skin care benefits of retinol; significantly reduce sagging, while tightening the skin and reducing uneven skin tone in middle-aged women. Compounding this Pseudostellariae cyclopeptide with other ingredients can achieve a synergistic effect and greatly enhance the anti-wrinkle effect of the anti-wrinkle cream.

[0026] Further, in some embodiments of the present application, the conocyclic peptide compounded in the anti-wrinkle cream is a biomimetic cyclic peptide derived from natural cono polypeptide, which is highly stable and has strong biological activity. It blocks ion channels through the spatial and electrostatic effects of the disulfide bond skeleton, thereby selectively inhibiting voltage-gated sodium channels. It can reduce the efficiency of excited cells generating action potentials, thereby reducing the efficiency of electrical signal propagation in nerves. It can significantly improve the smoothness of the skin and improve the roughness of the skin, increase the activity of fibroblasts, promote the generation of type I collagen, and increase the elasticity of the skin. Compounding this conocyclic peptide with other ingredients can achieve a synergistic effect, greatly enhancing the anti-wrinkle effect of the anti-wrinkle cream.

[0027] Further, in some embodiments of the present application, the Pichia recombinant collagen compounded in the anti-wrinkle cream uses the amino acids 1076-1202 of human type I collagen and the amino acids 594-846 of type III collagen as templates for human collagen. The nucleotide sequence of collagen was optimized by the translation pause theory, and a recombinant human collagen gene expression vector was constructed by molecular biology technology. The recombinant human collagen was soluble expressed in yeast cells, and high-purity recombinant human collagen was separated and purified. Collagen can be used for the treatment of burns, trauma and corneal diseases; secondly, it is also widely used in beauty, tissue repair, drug sustained release agents, etc. In the field of cosmetics, collagen is an important component of the extracellular matrix of the skin, and has the effects of moisturizing, strengthening the skin barrier, supplementing skin collagen, and anti-aging. Compounding this Pichia recombinant collagen with other ingredients can achieve a synergistic effect and greatly enhance the anti-wrinkle effect of the anti-wrinkle cream.

[0028] Further, in some embodiments of the present application, the mixture of hydrolyzed collagen and hexapeptide-9 compounded in the anti-wrinkle cream mainly contains marine collagen oligopeptides, red seaweed extract, naturally fermented erythritol and RSPO glycerol, a high proportion of natural source ingredients, biodegradable, improves skin moisturizing and barrier function, promotes the expression of collagen I, collagen III, collagen IV and collagen XVII, freezes age and resists early aging, and makes the skin smooth and delicate. Compounding this mixture of hydrolyzed collagen and hexapeptide-9 with other ingredients can achieve a synergistic effect and greatly enhance the anti-wrinkle effect of the anti-wrinkle cream.

[0029] The four active ingredients in the anti-wrinkle cream of the present application not only achieve the effect of mutual cooperation and synergistic enhancement, but also have high biological activity and strong stability when these four active ingredients are formulated together, thereby further enhancing the anti-wrinkle effect of the anti-wrinkle cream. Furthermore, the four active ingredients in the anti-wrinkle cream achieve multi-channel and multi-target anti-wrinkle from different anti-wrinkle channels, greatly improving the anti-wrinkle effect of the anti-wrinkle cream.

[0030] Furthermore, in some embodiments of the present application, the Pseudostellariae cyclic peptide solution includes: glycerol, water, and Pseudostellariae cyrtonema extract.

[0031] It should be noted that the Pseudostellariae Radix extract in the Pseudostellariae Radix cyclopeptide solution is mainly the biologically active cyclopeptide Heterophyllin B present in Pseudostellariae Radix.

[0032] Further optionally, in some embodiments of the present application, in the above-mentioned Pseudostellariae cyclic peptide solution, the mass percentage of Pseudostellariae cyclic peptide extract is 0.1% to 0.3%. Exemplarily, in some embodiments of the present application, in the above-mentioned Pseudostellariae cyclic peptide solution, the mass percentage of Pseudostellariae cyclic peptide extract is 0.1%, 0.11%, 0.12%, 0.13%, 0.15%, 0.16%, 0.17%, 0.18%, 0.19%, 0.2%, 0.22%, 0.23%, 0.24%, 0.25%, 0.26%, 0.28%, 0.3% or the range between any two of the aforementioned values.

[0033] As for the glycerol and water in the Pseudostellariae cyclic peptide solution, both are used as solvents, and there is no restriction on the content ratio of the two; they can be added according to actual needs.

[0034] Further optionally, in some embodiments of the present application, the composition of the above-mentioned Pseudostellariae cyclic peptide solution is, by mass percentage: 75-85% glycerol; 15-25% water; 0.1-0.3% Pseudostellariae extract.

[0035] For example, in some embodiments of the present application, the above-mentioned Pseudostellariae cyclic peptide can be selected from the Pseudostellariae cyclic peptide with the trade name CycloRetinTM.

[0036] Furthermore, in some embodiments of the present application, the conocyclic peptide solution includes: water, 1,2-pentanediol, 1,2-hexanediol, and conocyclic peptide.

[0037] It should be noted that the cono peptide in the above-mentioned cono cyclic peptide solution is mainly a biomimetic cyclic peptide derived from natural cono polypeptide.

[0038] Further optionally, in some embodiments of the present application, in the above-mentioned conocyclic peptide solution, the mass percentage of cono peptide is 0.1% to 5% by mass percentage. Exemplarily, in some embodiments of the present application, in the above-mentioned conocyclic peptide solution, the mass percentage of cono peptide is 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.8%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5% or the range between any two of the above values. Further optionally, in the above-mentioned conocyclic peptide solution, the mass percentage of cono peptide is 0.1% to 0.3%.

[0039] The water, 1,2-pentanediol and 1,2-hexanediol in the conocyclic peptide solution are all used as solvents, and there is no restriction on the content ratio of each solvent; they can be added according to actual needs.

[0040] Further optionally, in some embodiments of the present application, the composition of the above-mentioned conotoxin solution is, by mass percentage: 35-45% 1,2-pentanediol; 35-45% 1,2-hexanediol; 15-25% water; 0.1% to 10% Pseudostellariae extract. Exemplarily, in some embodiments of the present application, the above-mentioned conotoxin is selected from the conotoxin produced by Shenzhen Vicky Technology Co., Ltd. under the trade name Mu-conotoxin CnIIIC.

[0041] Furthermore, in some embodiments of the present application, the Pichia pastoris recombinant collagen solution includes: water, glycerol, sodium dihydrogen phosphate, sodium hydrogen phosphate, and soluble collagen.

[0042] It should be noted that the soluble collagen in the above-mentioned Pichia pastoris recombinant collagen solution is based on the template of human collagen at amino acids 1076-1202 of human type I collagen and amino acids 594-846 of type III collagen. The nucleotide sequence of collagen was optimized by translation pause theory, and a recombinant human collagen gene expression vector was constructed by molecular biology technology, and recombinant human collagen was soluble expressed in Pichia pastoris cells, and high-purity recombinant human collagen was separated and purified.

[0043] Further optionally, in some embodiments of the present application, in the above-mentioned Pichia recombinant collagen solution, the mass percentage of soluble collagen is 0.1% to 5% by mass percentage. Exemplarily, in some embodiments of the present application, in the above-mentioned Pichia recombinant collagen solution, the mass percentage of soluble collagen is 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.8%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5% or the range between any two of the above values. Further optionally, in the Pichia recombinant collagen solution, the mass percentage of soluble collagen is 0.1% to 0.3%. For water, glycerol, sodium dihydrogen phosphate, and disodium hydrogen phosphate in the Pichia recombinant collagen solution; they are all used as solvents, and the respective content ratios are not limited; they can be added according to actual needs. For example, in some embodiments of the present application, the above-mentioned Pichia pastoris recombinant collagen solution is selected from the Pichia pastoris recombinant collagen with the trade name HydroCol-y produced by Guangdong MaruMei Biotechnology Co., Ltd.

[0044] Further optionally, in some embodiments of the present application, the soluble collagen in the above-mentioned Pichia pastoris recombinant collagen solution is prepared according to the method in patent CN110484519B "Application of Pichia pastoris translation-related factor Bcy1 in foreign protein expression".

[0045] Further, in some embodiments of the present application, the mixture solution of hydrolyzed collagen and hexapeptide-9 includes: water, glycerin, sodium PCA, erythritol, crispy chondrus crispus powder, xanthan gum, hydrolyzed collagen, hexapeptide-9, benzoic acid, and sorbic acid.

[0046] It should be noted that the Chondrus crispus powder in the above-mentioned mixture solution is mainly red seaweed extract; and Hexapeptide-9 mainly contains marine collagen oligopeptides.

[0047] Further optionally, in some embodiments of the present application, in the above-mentioned mixture solution, the total mass percentage of hydrolyzed collagen powder (CHONDRUS CRISPUS) and hexapeptide-9 is 0.1% to 5% by mass percentage. Exemplarily, in some embodiments of the present application, in the above-mentioned mixture solution, the total mass percentage of hydrolyzed collagen powder and hexapeptide-9 is 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.8%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5% or the range between any two of the above values. Hydrolyzed collagen powder and hexapeptide-9 can be mixed in any proportion. Further optionally, in the above-mentioned mixture solution, the total mass percentage of hydrolyzed collagen powder (CHONDRUSCRISPUS) and hexapeptide-9 is 0.1% to 0.3%.

[0048] Optionally, in some embodiments of the present application, the mass ratio of hydrolyzed collagen powder to hexapeptide-9 is 1:(0.1-2).

[0049] Illustratively, in some embodiments of the present application, the mass ratio of hydrolyzed collagen powder to hexapeptide-9 is 1:0.1, 1:0.5, 1:1, 1:1.5, 2:1 or a range between any two of the foregoing values.

[0050] The water, glycerin, sodium PCA, erythritol, xanthan gum, hydrolyzed collagen, benzoic acid and sorbic acid in the aforementioned mixture solution of hydrolyzed collagen powder and hexapeptide-9 are all used as solvents, and their respective content ratios are not limited; they can be added according to actual needs.

[0051] For example, in some embodiments of the present application, the above-mentioned mixture of hydrolyzed collagen and hexapeptide-9 can be selected from a raw material with the trade name of Natriance Ageles extract.

[0052] Furthermore, in some embodiments of the present application, the anti-wrinkle cream further comprises: a primary emulsifier; the primary emulsifier is a low melting point C12-20 acid PEG-8 ester.

[0053] In the above technical scheme, the anti-wrinkle cream can be compounded with the aforementioned functional ingredients by using the low melting point C12-20 acid PEG-8 ester, which is not only beneficial to the anti-wrinkle effect of the entire anti-wrinkle cream, but also beneficial to the characteristic that the paste of the entire anti-wrinkle cream melts quickly after contacting the skin, thereby facilitating the skin absorption effect.

[0054] Furthermore, in some embodiments of the present application, the anti-wrinkle cream further comprises: at least one of avocado butter and cetearyl alcohol.

[0055] In the above technical scheme, the anti-wrinkle cream is compounded with avocado butter and cetearyl alcohol; it can further achieve a synergistic effect with the low-melting-point C12-20 acid PEG-8 ester, further enhancing the characteristic that the paste of the entire anti-wrinkle cream melts quickly after contacting the skin, thereby facilitating skin absorption.

[0056] Furthermore, in some embodiments of the present application, the anti-wrinkle cream also includes: isononyl isononanoate, polydimethylsiloxane, and ammonium acryloyldimethyltaurate / VP copolymer.

[0057] In the above technical scheme, the oil phase of the anti-wrinkle cream is mainly composed of isononyl isononanoate and polydimethylsiloxane, and is matched with the main thickener of the system, Aristoflex AVC (ammonium acryloyldimethyltaurate / VP copolymer), which can be coordinated with the aforementioned main emulsifier to make the anti-wrinkle cream paste light in texture and silky to the touch; thus, it is beneficial to the skin feel of the anti-wrinkle cream and the skin absorption effect.

[0058] Further optionally, in some embodiments of the present application, the composition of the above anti-wrinkle cream is as shown in Table 1:

[0059] Table 1 Anti-wrinkle cream

[0060]

[0061]

[0062]

[0063]

[0064] In the above table, in raw material No. 26, the mass percentage of Pseudostellariae Radix extract is 0.2%; in raw material No. 27, the mass percentage of conopeptide is 0.2%; in raw material No. 28, the mass percentage of soluble collagen is 0.2%; in raw material No. 29, the total mass percentage of hydrolyzed collagen and hexapeptide-9 is 0.2%, and the mass ratio of hydrolyzed collagen to hexapeptide-9 is 1:1. In some embodiments of the present application, the above anti-wrinkle cream is prepared according to the following steps:

[0065] Preparation process:

[0066] 1. Preparation (A): Add raw material (19) in Table 1 above to raw material (20), and disperse evenly with an external homogenizer until there are no particles for later use;

[0067] 2. Add raw material (1) into the water phase pot, start stirring at 1000 rpm, add raw materials (2), (3), (4), (5), (6), (7) and (8), raise the temperature to 80-85°C, and keep warm for 5-20 minutes;

[0068] 3. Add raw materials (9), (10), (11), (12), (13), (14), (15), (16) and (17) into the oil phase pot, start stirring at 1000 rpm, raise the temperature to 80-85°C, and keep warm for 5-15 minutes;

[0069] 4. Pump 3 / 4 of the water phase pot material into the emulsification pot, start the emulsification pot with maximum stirring, pump the oil phase pot material into the emulsification pot, and then pump the remaining 1 / 4 of the water phase pot material, vacuum - (0.03-0.05) Mpa, start external circulation homogenization for 3-8 minutes, 3000 rpm, after homogenization is completed, add (18) and start cooling;

[0070] 5. Reduce the stirring speed to 55 rpm. When the temperature drops to 40-45°C, add (21), (22), (23), (24), (25), (26), (27), (28), (29) and preformed (A), and maintain vacuum stirring for 5-15 minutes;

[0071] 6. Take samples for inspection. If qualified, filter the material with 100 mesh filter cloth at 35-40℃ (do not store in metal containers).

[0072] The anti-wrinkle cream of the present application has an innovative combination of bicyclic peptides; it is compounded with recombinant bicollagen and marine collagen oligopeptides to achieve a multi-dimensional anti-wrinkle and line-lightening effect (promoting collagen production, increasing SPARC in the dermis, increasing the level of fibrin in the dermis, inhibiting the transmission of skin nerve signals and inhibiting the activity of collagenase), effectively inhibiting skin aging. Furthermore, the anti-wrinkle cream of the present application has a light texture, has an excellent silky touch, melts quickly when in contact with the skin, absorbs quickly, is non-sticky and has a good moisturizing feel; further, the anti-wrinkle cream is compounded with the above-mentioned emulsifier and oil phase, making the entire cream mild and non-irritating, with a better skin feel, and the raw materials are natural and green and do not harm human skin.

[0073] The features and performance of the present application are further described in detail below in conjunction with the embodiments:

[0074] Example 1

[0075] Provided is an anti-wrinkle facial cream, the composition of which is shown in Table 2 below:

[0076] Table 2

[0077]

[0078]

[0079]

[0080]

[0081] In the above table, in raw material No. 26, the mass percentage of Pseudostellaria heterophylla extract is 0.2%; in raw material No. 27, the mass percentage of cono peptide is 0.2%; in raw material No. 28, the mass percentage of soluble collagen is 0.2%; in raw material No. 29, the total mass percentage of hydrolyzed collagen and hexapeptide-9 is 0.2%, and the mass ratio of hydrolyzed collagen to hexapeptide-9 is 1:1.

[0082] Preparation process:

[0083] 1. Preparing (A): Add raw material (19) in Table 2 to raw material (20), and disperse evenly with an external homogenizer until there are no particles;

[0084] 2. Add raw material (1) to the water phase pot, start stirring at 1000 rpm, add raw materials (2), (3), (4), (5), (6), (7) and (8), raise the temperature to 85°C, and keep warm for 10 minutes;

[0085] 3. Add raw materials (9), (10), (11), (12), (13), (14), (15), (16) and (17) into the oil phase pot, start stirring, rotate at 1000 rpm, raise the temperature to 85°C, and keep warm for 10 minutes;

[0086] 4. Pump 3 / 4 of the water phase pot material into the emulsification pot, start the emulsification pot with maximum stirring, pump the oil phase pot material into the emulsification pot, and then pump the remaining 1 / 4 of the water phase pot material, vacuum -0.04Mpa, start external circulation homogenization for 5 minutes, 3000rpm, after homogenization is completed, add (18) and start cooling;

[0087] 5. Reduce the stirring speed to 55 rpm. When the temperature drops to 45°C, add raw materials (21), (22), (23), (24), (25), (26), (27), (28), (29) and preformed (A), and maintain vacuum stirring for 10 minutes;

[0088] 6. Take samples for inspection. If qualified, filter the material with 100 mesh filter cloth at 40℃ (do not store in metal containers).

[0089]

Mildness test

[0090] 1. Patch test

[0091] Materials and methods

[0092] (1) Test substance: Anti-wrinkle cream sample of Example 1

[0093] (2) Negative control: blank control

[0094] (2) Subjects: 30 people (1 male, 29 females, aged between 20 and 60 years old)

[0095] (3) Patch method: Place 0.020-0.025 ml of the test substance in a patch device and apply it to the inner side of the subject's arm with a hypoallergenic tape. Remove the test substance after 24 hours and observe the skin reaction after 0.5 hours, 24 hours and 48 hours. Record the results according to the skin reaction grading standards in human patch tests in Chapter 7 of the 2015 edition of the "Technical Specifications for Safety of Cosmetics".

[0096] 1.2 Classification of adverse skin reactions (see Table 3)

[0097] Table 3

[0098]

[0099]

[0100] 1.3 The patch test results are shown in Table 4.

[0101] Table 4

[0102]

[0103] 1.4 Experimental Conclusion

[0104] The repairing cream samples prepared by adding the maximum amount of each ingredient in the anti-wrinkle cream were verified by patch test. The scores of 30 subjects after removing the test sample for 0.5h, 24h, and 48h were all 0. The scores of negative control samples after removing the negative control sample for 0.5h, 24h, and 48h were all 0. This shows that the anti-wrinkle cream is mild and non-irritating.

[0105] Example 2

[0106] Provided is an anti-wrinkle facial cream, the composition of which is shown in Table 5; the preparation process is the same as that of Example 1.

[0107] Table 5

[0108]

[0109]

[0110]

[0111] In the above table, in raw material No. 26, the mass percentage of Pseudostellaria heterophylla extract is 0.2%; in raw material No. 27, the mass percentage of cono peptide is 0.2%; in raw material No. 28, the mass percentage of soluble collagen is 0.2%; in raw material No. 29, the total mass percentage of hydrolyzed collagen and hexapeptide-9 is 0.2%, and the mass ratio of hydrolyzed collagen to hexapeptide-9 is 1:1.

[0112] Example 3

[0113] Provided is an anti-wrinkle facial cream, which is different from Example 2 in that: the content of raw material No. 27 is 1.0; the content of raw material No. 28 is 0.1; the content of raw material No. 29 is 1.0; and the rest are the same.

[0114] Comparative Example 1

[0115] A facial cream is provided, the composition of which is shown in Table 6; the preparation process is the same as that of Example 1.

[0116] Table 6

[0117]

[0118]

[0119]

[0120] Comparative Example 2

[0121] A facial cream is provided, which is different from Example 2 in that the content of raw material No. 26 is 0; and the rest are the same.

[0122] Comparative Example 3

[0123] A facial cream is provided, which is different from Example 2 in that the content of raw material No. 27 is 0; and the rest are the same.

[0124] Comparative Example 4

[0125] A facial cream is provided, which is different from Example 2 in that the content of raw material No. 28 is 0; and the rest are the same.

[0126] Comparative Example 5

[0127] A facial cream is provided, which is different from Example 2 in that the content of raw material No. 29 is 0; and the rest are the same.

[0128]

Anti-wrinkle efficacy test

[0129] The anti-wrinkle efficacy of the facial creams of Example 2 and Comparative Example 1 was tested.

[0130] Test plan:

[0131] (1) Test purpose

[0132] We set up our own before-and-after control test, and used non-invasive testing methods. After taking photos with Evaskin-S5, we analyzed the number / volume / area of ​​nasolabial folds and chuan-shaped wrinkles to evaluate the anti-wrinkle effect of the product.

[0133] (2) Product Usage

[0134] 2.1 Part of application: face

[0135] 2.2 How to use: After cleansing your face every morning and evening, take an appropriate amount of anti-wrinkle cream in the palm of your hand, apply it evenly on the entire face, gently pat until absorbed, and then gently lift and massage until completely absorbed.

[0136] 2.3 Use period: 28 days

[0137] (3) Selection criteria:

[0138] Ⅰ. Healthy Chinese women;

[0139] Ⅱ. Age 30 to 60 years old;

[0140] Ⅲ. There are nasolabial folds and Sichuan-shaped lines on the face;

[0141] IV. Subjects who are currently using hormone replacement therapy or hormone birth control should be able to ensure that they maintain their original usage habits during the trial period, and subjects who have not used such hormones should ensure that they do not use such hormones during the trial period;

[0142] Ⅴ. No allergic diseases, no history of allergy to cosmetics and other external preparations;

[0143] Ⅵ. Participate in the trial voluntarily, comply with the trial requirements, and provide timely feedback if any discomfort occurs.

[0144] (4) Test contents are shown in Table 7

[0145] Table 7

[0146] Serial number Experimental parameters Test Method Test site 1 Nasal folds and wrinkles Evaskin-S5 image acquisition and analysis Nasal fold area 2 Wrinkles Evaskin-S5 image acquisition and analysis Sichuan character pattern area

[0147] (5) Test results

[0148] Ⅰ. Each subject used the test product of each embodiment or comparative example for 28 days. The instrument test results are shown in Table 8:

[0149] Table 8

[0150]

[0151]

[0152] From the above table data, we can see that:

[0153] After using the test product of the embodiment of the present application for 28 days, the number / area / volume of nasolabial folds and the number / area / volume of C-shaped wrinkles were significantly improved compared with before use, which shows that the anti-wrinkle cream of the present application has significant anti-wrinkle effect.

[0154] However, the comparison product had no effect on the number / area / volume of nasolabial folds and the number / area / volume of crisscross lines before and after use, which shows that the comparison product has no anti-wrinkle effect.

[0155] From the comparison of Comparative Examples 2 to 5 with Example 2, it can be seen that the anti-wrinkle cream of the present application, which includes a mixture of Pseudomonas aeruginosa cyclic peptide, conospiro cyclic peptide, Pichia pastoris recombinant collagen, hydrolyzed collagen and hexapeptide-9, has four effective ingredients that work synergistically to achieve a synergistic effect and greatly improve the anti-wrinkle effect.

[0156] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. An anti-wrinkle cream, characterized in that: In terms of mass percentage, the anti-wrinkle cream comprises: 0.3%-2.5% of Pseudostellariae cyclic peptide solution, 1.0%-5.0% of conocyclic peptide solution, 0.1%-5.0% of Pichia pastoris recombinant collagen solution, and 1.0%-5.0% of a mixed solution of hydrolyzed collagen and hexapeptide-9.

2. The anti-wrinkle cream according to claim 1, characterized in that In terms of mass percentage, the anti-wrinkle cream comprises: 0.5%-2.0% of Pseudostellariae cyclic peptide solution, 1.5%-4.0% of conocyclic peptide solution, 0.5%-4.0% of Pichia pastoris recombinant collagen solution, and 1.5%-5.0% of a mixed solution of hydrolyzed collagen and hexapeptide-9.

3. The anti-wrinkle cream according to claim 1, characterized in that The Pseudostellariae Radix Cyclopeptide Solution comprises: glycerol, water, and Pseudostellariae Radix Extract; Optionally, in the Pseudostellariae Radix cyclopeptide solution, the mass percentage of the Pseudostellariae Radix extract is 0.1% to 0.3%.

4. The anti-wrinkle cream according to claim 1, characterized in that The conocyclic peptide solution comprises: water, 1,2-pentanediol, 1,2-hexanediol, and conocyclic peptide; Optionally, the mass percentage of the cono peptide in the cono peptide solution is 0.1% to 5%.

5. The anti-wrinkle cream according to claim 1, characterized in that The Pichia pastoris recombinant collagen solution comprises: water, glycerol, sodium dihydrogen phosphate, disodium hydrogen phosphate, and soluble collagen; Optionally, in the Pichia pastoris recombinant collagen solution, the mass percentage of the soluble collagen is 0.1% to 5%.

6. The anti-wrinkle cream according to claim 5, characterized in that The Pichia pastoris recombinant collagen uses the amino acids 1076-1202 of human type I collagen and the amino acids 594-846 of type III collagen as templates of human collagen.

7. The anti-wrinkle cream according to claim 1, characterized in that The mixed solution of hydrolyzed collagen and hexapeptide-9 comprises: water, glycerin, sodium PCA, erythritol, crispy chondrus crispus powder, xanthan gum, hydrolyzed collagen, hexapeptide-9, benzoic acid, and sorbic acid; Optionally, in the mixed solution, the total mass percentage of the hydrolyzed collagen and the hexapeptide-9 is 0.1% to 5%.

8. The anti-wrinkle cream according to any one of claims 1 to 7, characterized in that The anti-wrinkle cream also includes: a main emulsifier; the main emulsifier is a low melting point C12-20 acid PEG-8 ester.

9. The anti-wrinkle cream according to any one of claims 1 to 7, characterized in that The anti-wrinkle cream further comprises: shea butter and cetearyl alcohol.

10. The anti-wrinkle cream according to any one of claims 1 to 7, characterized in that The anti-wrinkle cream also includes: isononyl isononanoate, polydimethylsiloxane, and ammonium acryloyldimethyltaurate / VP copolymer.

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