Preparation method of composition added with natural NMF moisturizing factor

Abstract

The invention relates to the technical field of cosmetic preparation, in particular to a preparation method of a composition added with a natural NMF moisturizing factor. Comprising the following steps: S1.1, weighing the following raw materials in parts by weight: 10 to 12 parts of lipidosome enzyme, 21 to 25 parts of hyaluronic acid, 4 to 8 parts of urea, 1 to 3 parts of glycerol glucoside, 1 to 5 parts of sodium lactate, 5 to 7 parts of neoagarotetraose, 2 to 4 parts of ink algae glue, 1 to 3 parts of grape seed oil, 1 to 5 parts of Morocco nut oil and 0.4 to 0.8 part of stearyl alcohol; the composition is prepared from the following components in parts by weight: 5-9 parts of a sponge extract, 2-4 parts of a resurrection grass extract, 8-12 parts of a longan seed extract, 3-5 parts of a blackberry leaf extract, 0.5-0.7 part of a gulfweed extract and 50-70 parts of glacier water. Wherein the liposome enzyme is prepared by mixing polyphosphate kinase and lecithin according to a mass ratio of 1: (10-15); s1.2, preparing a water phase; and S1.3, preparing an oil phase and adding lipidosome enzyme to obtain the composition. Polyphosphokinase is adopted to strengthen the metabolic capability of cells, and lecithin is adopted to improve permeability and stability.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetic preparation, in particular to a method for preparing a composition added with a natural NMF moisturizing factor. Background Art

[0002] Natural Moisturizing Factor (NMF), full name Natural Moisturizing Factor, is a key component for retaining moisture in the stratum corneum of the skin. It is a mixture of low molecular weight water-soluble substances with hygroscopic and water-retaining properties. It is mainly composed of amino acids, pyrrolidone carboxylic acid (PCA), lactate, urea and other substances. These ingredients not only exist in the natural moisturizing system of the skin, but are also widely used in cosmetics due to their excellent skin-friendliness and low irritation. As an important moisturizing ingredient, the composition containing natural NMF moisturizing factor is designed to simulate and supplement the original NMF in the skin, thereby enhancing the skin's moisturizing ability and barrier function.

[0003] The natural moisturizing factor compositions currently on the market for moisturizing and hydrating achieve the effect of instant nourishing and moisturizing the skin by quickly replenishing skin moisture, creating an illusion that the skin has improved. However, the problem that the skin cannot be repaired in time after being damaged has not been effectively solved. In view of this, we propose a method for preparing a composition with added natural NMF moisturizing factor. Summary of the invention

[0004] The object of the present invention is to provide a method for preparing a composition added with natural NMF moisturizing factor to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides a method for preparing a composition with added natural NMF moisturizing factor, comprising the following steps: S1.1, weighing the following raw materials in parts by weight: 10-12 parts by weight of liposome enzyme, 21-25 parts by weight of hyaluronic acid, 4-8 parts by weight of urea, 1-3 parts by weight of glyceryl glucoside, 1-5 parts by weight of sodium lactate, 5-7 parts by weight of neoaganetetrose, 2-4 parts by weight of fucan, 1-3 parts by weight of grape seed oil, 1-5 parts by weight of argan oil, 0.4-0.8 parts by weight of stearyl alcohol, 5-9 parts by weight of sponge extract, 2-4 parts by weight of resurrection grass extract, 8-12 parts by weight of longan kernel extract, 3-5 parts by weight of blackberry leaf extract, 0.5-0.7 parts by weight of horsetail extract and 50-70 parts by weight of glacier water;

[0006] The liposome enzyme is prepared by mixing polyphosphokinase and lecithin in a mass ratio of 1:10-15;

[0007] S1.2, heat the glacier water to 75-85℃, add hyaluronic acid, urea, glyceryl glucoside, sodium lactate, neoagarnet, fucus algae, sponge extract, resurrection grass extract, longan kernel extract, blackberry leaf extract and horsetail extract in sequence while stirring, and continue stirring until all ingredients are completely dissolved;

[0008] S1.3. Heat grape seed oil and argan oil to 75-85°C, then add stearyl alcohol, stir thoroughly with a magnetic stirrer, then cool to 30-45°C, add liposome enzyme, and finally cool to room temperature to obtain a composition with added natural NMF moisturizing factor.

[0009] Fucoidan regulates the gene expression related to the formation of epidermal keratinocyte mantle, promotes the degradation of filaggrin (FLG) into natural moisturizing factor, enhances skin hydration activity and improves skin barrier; longan nuclear extract has excellent antioxidant activity, can effectively scavenge free radicals, effectively resists glycation and has good moisturizing activity; neoagar-tetraose can significantly increase the expression levels of filaggrin (FLG), aquaporin (AQP3) and tight junction protein (ZO-1) in keratinocytes, and has good utilization value in moisturizing; horsetail extract is rich in silicates, which can enhance the elasticity and firmness of the skin, and has moisturizing and anti-aging effects; glacier water is pure and rich in minerals, which can provide deep hydration and purification for the skin, and also has calming and soothing effects.

[0010] Preferably, the steps of preparing liposome enzyme are specifically as follows:

[0011] Dissolve lecithin and cholesterol in a mixture of chloroform and methanol to obtain an organic solution, stir thoroughly to ensure that the phospholipids are completely dissolved, transfer the organic solution in which the lecithin and cholesterol are dissolved to a round-bottom flask, use a rotary evaporator to perform rotary evaporation at 35-40°C to form a uniform dry phospholipid film, add buffer a preheated to the same temperature to the dry phospholipid film, oscillate to fully hydrate the phospholipid film to form a liposome suspension, the oscillation frequency is 100-200 times / min, then add polyphosphate kinase to the liposome suspension, perform ultrasonic treatment to encapsulate the polyphosphate kinase inside the liposome, put the liposome suspension containing the polyphosphate kinase into a dialysis bag, place it in buffer b for dialysis, and obtain a modified product liposome enzyme.

[0012] Polyphosphate kinase can use polyphosphate as a substrate to synthesize adenosine triphosphate (ATP), and plays an important role in the energy metabolism and ATP regeneration process in the body. Since ATP is the main form of cellular energy, it can enhance cell vitality, thereby promoting skin cell metabolism and repair function.

[0013] Liposomal enzyme is prepared by coating polyphosphate kinase with lecithin lipid. Lipidation can not only improve the permeability of polyphosphate kinase, but also the liposome can encapsulate polyphosphate kinase and protect it from degradation.

[0014] Sponge extracts are rich in ceramide compounds and inorganic polyphosphates. Inorganic polyphosphates can interact with liposome enzymes and promote the synthesis of adenosine triphosphate (ATP) from inorganic polyphosphates.

[0015] Preferably, the mass ratio of lecithin to cholesterol is 10:0.8-1.3.

[0016] Preferably, in the mixed solution of chloroform and methanol, the volume ratio of chloroform to methanol is 2 to 3:1.

[0017] Preferably, the concentration of the lecithin in the mixture of chloroform and methanol is 5-7 mg / mL.

[0018] Preferably, the buffer a and the buffer b are both phosphate buffers, and the mass ratio of the buffer to the phospholipid film is 1:11-13.

[0019] Preferably, the ultrasonic treatment is performed for a time of 20-30 minutes and a power of 20-50W.

[0020] Preferably, the molecular cut-off of the dialysis bag is 10-30 kDa.

[0021] Preferably, the dialysis time is 12-24 hours, and the dialysis temperature is controlled at 3-5°C.

[0022] Preferably, the pH value of the phosphate buffer is 7.0-7.5, and the buffer is replaced every 4-6 hours.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] In the preparation method of the composition added with natural NMF moisturizing factor, polyphosphate kinase and lecithin are used to prepare liposome enzyme, and the ability of polyphosphate kinase to synthesize adenosine triphosphate is utilized to enhance the vitality of cells, thereby achieving the goal of promoting skin cell metabolism and repair function. The polyphosphate kinase is encapsulated by lecithin, which improves the permeability of the polyphosphate kinase on the one hand, and improves the stability of the polyphosphate kinase on the other hand. In combination with the inorganic polyphosphate contained in the sponge extract, the polyphosphate kinase can be promoted to synthesize adenosine triphosphate with the inorganic polyphosphate, strengthen the metabolic capacity of the cells, and achieve the ability to repair damaged skin tissue. At the same time, it synergizes with other moisturizing components to both moisturize and repair the barrier. DETAILED DESCRIPTION

[0025] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] A method for preparing a composition containing a natural NMF moisturizing factor according to the present invention comprises the following steps:

[0027] S1.1, weigh the following raw materials in parts by weight: 10-12 parts by weight of liposome enzyme, 21-25 parts by weight of hyaluronic acid, 4-8 parts by weight of urea, 1-3 parts by weight of glyceryl glucoside, 1-5 parts by weight of sodium lactate, 5-7 parts by weight of neotetraose, 2-4 parts by weight of fucose, 1-3 parts by weight of grape seed oil, 1-5 parts by weight of argan oil, 0.4-0.8 parts by weight of stearyl alcohol, 5-9 parts by weight of sponge extract, 2-4 parts by weight of resurrection grass extract, 8-12 parts by weight of longan kernel extract, 3-5 parts by weight of blackberry leaf extract, 0.5-0.7 parts by weight of horsetail extract and 50-70 parts by weight of glacial water;

[0028] The liposome enzyme is prepared by mixing polyphosphokinase and lecithin in a mass ratio of 1:10-15;

[0029] S1.2, heat the glacier water to 75-85℃, add hyaluronic acid, urea, glyceryl glucoside, sodium lactate, neoagarnet, fucus algae, sponge extract, resurrection grass extract, longan kernel extract, blackberry leaf extract and horsetail extract in sequence while stirring, and continue stirring until all ingredients are completely dissolved;

[0030] S1.3. Heat grape seed oil and argan oil to 75-85°C, then add stearyl alcohol, stir thoroughly with a magnetic stirrer, then cool to 30-45°C, add liposome enzyme, and finally cool to room temperature to obtain a composition with added natural NMF moisturizing factor.

[0031] Embodiment 1:

[0032] A method for preparing a composition containing a natural NMF moisturizing factor, comprising the following steps:

[0033] S1.1, weigh the following raw materials in parts by weight: 12 parts by weight of liposome enzyme, 25 parts by weight of hyaluronic acid, 8 parts by weight of urea, 3 parts by weight of glyceryl glucoside, 5 parts by weight of sodium lactate, 7 parts by weight of neotetraose, 4 parts by weight of fucose, 3 parts by weight of grape seed oil, 5 parts by weight of argan oil, 0.8 parts by weight of stearyl alcohol, 9 parts by weight of sponge extract, 4 parts by weight of resurrection grass extract, 12 parts by weight of longan kernel extract, 5 parts by weight of blackberry leaf extract, 0.7 parts by weight of horsetail extract and 70 parts by weight of glacial water;

[0034] The liposome enzyme was prepared by mixing polyphosphokinase and phosphatidylcholine in a mass ratio of 1:10; the mass ratio of phosphatidylcholine to cholesterol was 10:1.3; in the mixture of chloroform and methanol, the volume ratio of chloroform to methanol was 3:1; buffer a and buffer b were both phosphate buffers, and the mass ratio of buffer to phospholipid film was 1:13;

[0035] Dissolve lecithin and cholesterol in a mixture of chloroform and methanol to obtain an organic solution, wherein the concentration of lecithin in the mixture of chloroform and methanol is 7 mg / mL, stir thoroughly to ensure that the phospholipids are completely dissolved, transfer the organic solution in which lecithin and cholesterol are dissolved to a round-bottom flask, use a rotary evaporator to rotary evaporate at 40°C to form a uniform dry phospholipid film, add buffer a preheated to the same temperature to the dry phospholipid film, oscillate to fully hydrate the phospholipid film to form a liposome suspension, the oscillation frequency is 150 times / min, then add polyphosphate kinase to the liposome suspension, treat by ultrasound, the ultrasound time is 20 min, the power is set to 30 W, so that the polyphosphate kinase is wrapped inside the liposome, the liposome suspension containing the polyphosphate kinase is placed in a dialysis bag, placed in buffer b for dialysis, the pH value of the phosphate buffer is 7.2, the molecular cutoff of the dialysis bag is 10 kDa, the dialysis time is 24 h, the dialysis temperature is controlled at 4°C, and the buffer is replaced every 4 h to obtain a modified product liposome enzyme;

[0036] S1.2, heat the glacial water to 80°C, add hyaluronic acid, urea, glyceryl glucoside, sodium lactate, neotetraose, fucus algae, sponge extract, resurrection grass extract, longan kernel extract, blackberry leaf extract and horsetail extract in sequence while stirring, and continue stirring until all ingredients are completely dissolved;

[0037] S1.3. Heat grape seed oil and argan oil to 80°C, then add stearyl alcohol, stir thoroughly with a magnetic stirrer, then cool to 40°C, add liposome enzyme, and finally cool to room temperature to obtain a composition with added natural NMF moisturizing factor.

[0038] Embodiment 2:

[0039] A method for preparing a composition containing a natural NMF moisturizing factor, comprising the following steps:

[0040] S1.1, weigh the following raw materials in parts by weight: 12 parts by weight of liposome enzyme, 25 parts by weight of hyaluronic acid, 8 parts by weight of urea, 3 parts by weight of glyceryl glucoside, 5 parts by weight of sodium lactate, 7 parts by weight of neotetraose, 4 parts by weight of fucose, 3 parts by weight of grape seed oil, 5 parts by weight of argan oil, 0.8 parts by weight of stearyl alcohol, 9 parts by weight of sponge extract, 4 parts by weight of resurrection grass extract, 12 parts by weight of longan kernel extract, 5 parts by weight of blackberry leaf extract, 0.7 parts by weight of horsetail extract and 70 parts by weight of glacial water;

[0041] The liposome enzyme was prepared by mixing polyphosphokinase and phosphatidylcholine in a mass ratio of 1:13; the mass ratio of phosphatidylcholine to cholesterol was 10:1.3; in the mixture of chloroform and methanol, the volume ratio of chloroform to methanol was 3:1; buffer a and buffer b were both phosphate buffers, and the mass ratio of buffer to phospholipid film was 1:13;

[0042] Dissolve lecithin and cholesterol in a mixture of chloroform and methanol to obtain an organic solution, wherein the concentration of lecithin in the mixture of chloroform and methanol is 7 mg / mL, stir thoroughly to ensure that the phospholipids are completely dissolved, transfer the organic solution in which lecithin and cholesterol are dissolved to a round-bottom flask, use a rotary evaporator to rotary evaporate at 40°C to form a uniform dry phospholipid film, add buffer a preheated to the same temperature to the dry phospholipid film, oscillate to fully hydrate the phospholipid film to form a liposome suspension, the oscillation frequency is 150 times / min, then add polyphosphate kinase to the liposome suspension, treat by ultrasound, the ultrasound time is 20 min, the power is set to 30 W, so that the polyphosphate kinase is wrapped inside the liposome, the liposome suspension containing the polyphosphate kinase is placed in a dialysis bag, placed in buffer b for dialysis, the pH of the phosphate buffer is 7.2, the molecular cutoff of the dialysis bag is 10 kDa, the dialysis time is 24 h, the dialysis temperature is controlled at 4°C, and the buffer is replaced every 4 h to obtain a modified product liposome enzyme;

[0043] S1.2, heat the glacial water to 80°C, add hyaluronic acid, urea, glyceryl glucoside, sodium lactate, neotetraose, fucus algae, sponge extract, resurrection grass extract, longan kernel extract, blackberry leaf extract and horsetail extract in sequence while stirring, and continue stirring until all ingredients are completely dissolved;

[0044] S1.3. Heat grape seed oil and argan oil to 80°C, then add stearyl alcohol, stir thoroughly with a magnetic stirrer, then cool to 40°C, add liposome enzyme, and finally cool to room temperature to obtain a composition with added natural NMF moisturizing factor.

[0045] Embodiment 3:

[0046] A method for preparing a composition containing a natural NMF moisturizing factor, comprising the following steps:

[0047] S1.1, weigh the following raw materials in parts by weight: 12 parts by weight of liposome enzyme, 25 parts by weight of hyaluronic acid, 8 parts by weight of urea, 3 parts by weight of glyceryl glucoside, 5 parts by weight of sodium lactate, 7 parts by weight of neotetraose, 4 parts by weight of fucose, 3 parts by weight of grape seed oil, 5 parts by weight of argan oil, 0.8 parts by weight of stearyl alcohol, 9 parts by weight of sponge extract, 4 parts by weight of resurrection grass extract, 12 parts by weight of longan kernel extract, 5 parts by weight of blackberry leaf extract, 0.7 parts by weight of horsetail extract and 70 parts by weight of glacial water;

[0048] The liposome enzyme was prepared by mixing polyphosphokinase and phosphatidylcholine in a mass ratio of 1:15; the mass ratio of phosphatidylcholine to cholesterol was 10:1.3; in the mixture of chloroform and methanol, the volume ratio of chloroform to methanol was 3:1; buffer a and buffer b were both phosphate buffers, and the mass ratio of buffer to phospholipid film was 1:13;

[0049] Dissolve lecithin and cholesterol in a mixture of chloroform and methanol to obtain an organic solution, wherein the concentration of lecithin in the mixture of chloroform and methanol is 7 mg / mL, stir thoroughly to ensure that the phospholipids are completely dissolved, transfer the organic solution in which lecithin and cholesterol are dissolved to a round-bottom flask, use a rotary evaporator to rotary evaporate at 40°C to form a uniform dry phospholipid film, add buffer a preheated to the same temperature to the dry phospholipid film, oscillate to fully hydrate the phospholipid film to form a liposome suspension, the oscillation frequency is 150 times / min, then add polyphosphate kinase to the liposome suspension, treat by ultrasound, the ultrasound time is 20 min, the power is set to 30 W, so that the polyphosphate kinase is wrapped inside the liposome, the liposome suspension containing the polyphosphate kinase is placed in a dialysis bag, placed in buffer b for dialysis, the pH of the phosphate buffer is 7.2, the molecular cutoff of the dialysis bag is 10 kDa, the dialysis time is 24 h, the dialysis temperature is controlled at 4°C, and the buffer is replaced every 4 h to obtain a modified product liposome enzyme;

[0050] S1.2, heat the glacial water to 80°C, add hyaluronic acid, urea, glyceryl glucoside, sodium lactate, neotetraose, fucus algae, sponge extract, resurrection grass extract, longan kernel extract, blackberry leaf extract and horsetail extract in sequence while stirring, and continue stirring until all ingredients are completely dissolved;

[0051] S1.3. Heat grape seed oil and argan oil to 80°C, then add stearyl alcohol, stir thoroughly with a magnetic stirrer, then cool to 40°C, add liposome enzyme, and finally cool to room temperature to obtain a composition with added natural NMF moisturizing factor.

[0052] Embodiment 4:

[0053] A method for preparing a composition containing a natural NMF moisturizing factor, comprising the following steps:

[0054] S1.1, weigh the following raw materials in parts by weight: 12 parts by weight of liposome enzyme, 21 parts by weight of hyaluronic acid, 4 parts by weight of urea, 1 part by weight of glyceryl glucoside, 1 part by weight of sodium lactate, 5 parts by weight of neotetraose, 2 parts by weight of fucoseed gel, 1 part by weight of grape seed oil, 1 part by weight of argan oil, 0.4 parts by weight of stearyl alcohol, 5 parts by weight of sponge extract, 2 parts by weight of resurrection grass extract, 8 parts by weight of longan kernel extract, 3 parts by weight of blackberry leaf extract, 0.5 parts by weight of horsetail extract and 50 parts by weight of glacial water;

[0055] The liposome enzyme was prepared by mixing polyphosphokinase and phosphatidylcholine in a mass ratio of 1:15; the mass ratio of phosphatidylcholine to cholesterol was 10:0.8; in the mixture of chloroform and methanol, the volume ratio of chloroform to methanol was 2:1; buffer a and buffer b were both phosphate buffers, and the mass ratio of buffer to phospholipid film was 1:11;

[0056] Dissolve lecithin and cholesterol in a mixture of chloroform and methanol to obtain an organic solution, wherein the concentration of lecithin in the mixture of chloroform and methanol is 7 mg / mL, stir thoroughly to ensure that the phospholipids are completely dissolved, transfer the organic solution in which lecithin and cholesterol are dissolved to a round-bottom flask, use a rotary evaporator to rotary evaporate at 40°C to form a uniform dry phospholipid film, add buffer a preheated to the same temperature to the dry phospholipid film, oscillate to fully hydrate the phospholipid film to form a liposome suspension, the oscillation frequency is 150 times / min, then add polyphosphate kinase to the liposome suspension, treat by ultrasound, the ultrasound time is 20 min, the power is set to 30 W, so that the polyphosphate kinase is wrapped inside the liposome, the liposome suspension containing the polyphosphate kinase is placed in a dialysis bag, placed in buffer b for dialysis, the pH value of the phosphate buffer is 7.2, the molecular cutoff of the dialysis bag is 10 kDa, the dialysis time is 24 h, the dialysis temperature is controlled at 4°C, and the buffer is replaced every 4 h to obtain a modified product liposome enzyme;

[0057] S1.2, heat the glacial water to 80°C, add hyaluronic acid, urea, glyceryl glucoside, sodium lactate, neotetraose, fucus algae, sponge extract, resurrection grass extract, longan kernel extract, blackberry leaf extract and horsetail extract in sequence while stirring, and continue stirring until all ingredients are completely dissolved;

[0058] S1.3. Heat grape seed oil and argan oil to 80°C, then add stearyl alcohol, stir thoroughly with a magnetic stirrer, then cool to 40°C, add liposome enzyme, and finally cool to room temperature to obtain a composition with added natural NMF moisturizing factor.

[0059] Embodiment 5:

[0060] A method for preparing a composition containing a natural NMF moisturizing factor, comprising the following steps:

[0061] S1.1, weigh the following raw materials in parts by weight: 10 parts by weight of liposome enzyme, 25 parts by weight of hyaluronic acid, 8 parts by weight of urea, 3 parts by weight of glyceryl glucoside, 5 parts by weight of sodium lactate, 7 parts by weight of neotetraose, 4 parts by weight of fucose, 3 parts by weight of grape seed oil, 5 parts by weight of argan oil, 0.8 parts by weight of stearyl alcohol, 9 parts by weight of sponge extract, 4 parts by weight of resurrection grass extract, 12 parts by weight of longan kernel extract, 5 parts by weight of blackberry leaf extract, 0.7 parts by weight of horsetail extract and 70 parts by weight of glacial water;

[0062] The liposome enzyme was prepared by mixing polyphosphokinase and phosphatidylcholine in a mass ratio of 1:15; the mass ratio of phosphatidylcholine to cholesterol was 10:1.3; in the mixture of chloroform and methanol, the volume ratio of chloroform to methanol was 3:1; buffer a and buffer b were both phosphate buffers, and the mass ratio of buffer to phospholipid film was 1:13;

[0063] Dissolve lecithin and cholesterol in a mixture of chloroform and methanol to obtain an organic solution, wherein the concentration of lecithin in the mixture of chloroform and methanol is 7 mg / mL, stir thoroughly to ensure that the phospholipids are completely dissolved, transfer the organic solution in which lecithin and cholesterol are dissolved to a round-bottom flask, use a rotary evaporator to rotary evaporate at 40°C to form a uniform dry phospholipid film, add buffer a preheated to the same temperature to the dry phospholipid film, oscillate to fully hydrate the phospholipid film to form a liposome suspension, the oscillation frequency is 150 times / min, then add polyphosphate kinase to the liposome suspension, treat by ultrasound, the ultrasound time is 20 min, the power is set to 30 W, so that the polyphosphate kinase is wrapped inside the liposome, the liposome suspension containing the polyphosphate kinase is placed in a dialysis bag, placed in buffer b for dialysis, the pH value of the phosphate buffer is 7.2, the molecular cutoff of the dialysis bag is 10 kDa, the dialysis time is 24 h, the dialysis temperature is controlled at 4°C, and the buffer is replaced every 4 h to obtain a modified product liposome enzyme;

[0064] S1.2, heat the glacial water to 80°C, add hyaluronic acid, urea, glyceryl glucoside, sodium lactate, neotetraose, fucus algae, sponge extract, resurrection grass extract, longan kernel extract, blackberry leaf extract and horsetail extract in sequence while stirring, and continue stirring until all ingredients are completely dissolved;

[0065] S1.3. Heat grape seed oil and argan oil to 80°C, then add stearyl alcohol, stir thoroughly with a magnetic stirrer, then cool to 40°C, add liposome enzyme, and finally cool to room temperature to obtain a composition with added natural NMF moisturizing factor.

[0066] Embodiment 6:

[0067] A method for preparing a composition containing a natural NMF moisturizing factor, comprising the following steps:

[0068] S1.1, weigh the following raw materials in parts by weight: 11 parts by weight of liposome enzyme, 25 parts by weight of hyaluronic acid, 8 parts by weight of urea, 3 parts by weight of glyceryl glucoside, 5 parts by weight of sodium lactate, 7 parts by weight of neotetraose, 4 parts by weight of fucose, 3 parts by weight of grape seed oil, 5 parts by weight of argan oil, 0.8 parts by weight of stearyl alcohol, 9 parts by weight of sponge extract, 4 parts by weight of resurrection grass extract, 12 parts by weight of longan kernel extract, 5 parts by weight of blackberry leaf extract, 0.7 parts by weight of horsetail extract and 70 parts by weight of glacial water;

[0069] The liposome enzyme was prepared by mixing polyphosphokinase and phosphatidylcholine in a mass ratio of 1:15; the mass ratio of phosphatidylcholine to cholesterol was 10:1.3; in the mixture of chloroform and methanol, the volume ratio of chloroform to methanol was 3:1; buffer a and buffer b were both phosphate buffers, and the mass ratio of buffer to phospholipid film was 1:13;

[0070] Dissolve lecithin and cholesterol in a mixture of chloroform and methanol to obtain an organic solution, wherein the concentration of lecithin in the mixture of chloroform and methanol is 7 mg / mL, stir thoroughly to ensure that the phospholipids are completely dissolved, transfer the organic solution in which lecithin and cholesterol are dissolved to a round-bottom flask, use a rotary evaporator to rotary evaporate at 40°C to form a uniform dry phospholipid film, add buffer a preheated to the same temperature to the dry phospholipid film, oscillate to fully hydrate the phospholipid film to form a liposome suspension, the oscillation frequency is 150 times / min, then add polyphosphate kinase to the liposome suspension, treat by ultrasound, the ultrasound time is 20 min, the power is set to 30 W, so that the polyphosphate kinase is wrapped inside the liposome, the liposome suspension containing the polyphosphate kinase is placed in a dialysis bag, placed in buffer b for dialysis, the pH value of the phosphate buffer is 7.5, the molecular cutoff of the dialysis bag is 10 kDa, the dialysis time is 24 h, the dialysis temperature is controlled at 4°C, and the buffer is replaced every 4 h to obtain a modified product liposome enzyme;

[0071] S1.2, heat the glacial water to 80°C, add hyaluronic acid, urea, glyceryl glucoside, sodium lactate, neotetraose, fucus algae, sponge extract, resurrection grass extract, longan kernel extract, blackberry leaf extract and horsetail extract in sequence while stirring, and continue stirring until all ingredients are completely dissolved;

[0072] S1.3. Heat grape seed oil and argan oil to 80°C, then add stearyl alcohol, stir thoroughly with a magnetic stirrer, then cool to 40°C, add liposome enzyme, and finally cool to room temperature to obtain a composition with added natural NMF moisturizing factor.

[0073] Comparative Example 1: The method of Example 3 was adopted without using liposome enzyme.

[0074] Comparative Example 2: The method of Example 3 was adopted without using lecithin to modify the polyphosphate kinase.

[0075] Comparative Example 3: The method of Example 3 was adopted without adding sponge extract.

[0076] The present invention discloses a method for preparing a composition containing a natural NMF moisturizing factor by using liposome enzymes, wherein the performance index inspection items and inspection standards of the method for preparing a composition containing a natural NMF moisturizing factor are as follows:

[0077] 100 volunteers aged between 20 and 30 years old, regardless of gender, were selected and divided into 10 groups on average. The above test samples were used respectively, and the last group was a blank control group. The blank control group used deionized water as the test sample. The volunteers in each group wiped their faces with dry tissues. After cleaning, the measurement areas were marked on the left and right faces of the subjects. Before the formal test, the subjects sat quietly for 30 minutes in an environment with a temperature of 25±2°C and a humidity of 50±2%, without drinking water and keeping relaxed. 5mL of the test sample was applied to the test area, and the skin water content of the test area and the blank control area of ​​the subjects was measured at 30min and 180min. The test of the same group of subjects was completed by the same measurement personnel, and the test average value was calculated. The percentage increase in skin water content MMV (%) = (W 1 -W 0 ) / W 0 × 100%, where W 0 = moisture content of the skin before using the sample, W 1 = moisture content of the skin after using the sample.

[0078] According to the above standards, the preparation method of the composition with added natural NMF moisturizing factor prepared in the above Examples 1-6 and Comparative Examples 1-3 was tested, and the obtained data are shown in Table 1:

[0079] Table 1 Performance data of Examples 1-6 and Comparative Examples 1-3

[0080]

[0081] The above data fully show that compared with Comparative Examples 1-3, Examples 1-6 can fully show the effect of liposome enzyme on the preparation method of a composition with added natural NMF moisturizing factor in moisturizing and repairing.

[0082] Since the present invention uses liposome enzyme to prepare the composition with added natural NMF moisturizing factor, the liposome enzyme effectively improves the moisturizing and repairing properties of the composition, as follows:

[0083] It can be seen from Examples 1-3 that as the proportion of components in the liposome enzyme continues to increase, the moisturizing and repairing properties of the composition added with the natural NMF moisturizing factor gradually increase, indicating that lecithin can effectively promote the stability and permeability of polyphosphate kinase, so that polyphosphate kinase synthesizes more ATP, thereby increasing cell activity, promoting cell metabolism, and repairing damaged skin. The moisturizing ingredients increase the hydration level of the skin's stratum corneum and improve the skin's water retention capacity. Therefore, the composition added with the natural NMF moisturizing factor is not only effective in moisturizing, but also can effectively repair damaged cell barriers.

[0084] It can be seen from Examples 3 and 4 that as the content of other components in the composition to which the natural NMF moisturizing factor is added continues to change, the moisturizing and repairing properties of the composition to which the natural NMF moisturizing factor is added do not change significantly, indicating that small changes in other components within a certain range are not sufficient to significantly affect the moisturizing and repairing properties of the composition to which the natural NMF moisturizing factor is added.

[0085] It can be seen from Examples 3, 5 and 6 that the moisturizing and repairing properties of the composition to which the natural NMF moisturizing factor is added gradually increase, indicating that liposome enzymes can promote the synthesis of cellular ATP, thereby promoting cell vitality, thereby metabolizing damaged cells, and cooperating with other moisturizing components to form a protective film on the skin surface, thereby improving the water retention capacity and restoring the skin's barrier function, making the skin healthier.

[0086] According to the above test experiments, the composition prepared according to Example 3 with the addition of natural NMF moisturizing factor has the best performance, so Example 3 is taken as the best example;

[0087] By comparing Example 3 with Comparative Examples 1-3, it can be seen that:

[0088] In Comparative Example 1, liposome enzyme is not used, and the moisturizing and repairing properties of the composition with natural NMF moisturizing factor added are poor. The effect of liposome enzyme in enhancing cell vitality is lacking. Although other components can quickly replenish skin moisture and achieve the effect of instant nourishing and moisturizing, it is only an illusion that the skin has improved, and the problem is not fundamentally solved. Therefore, the moisturizing and repairing properties of the composition with natural NMF moisturizing factor added are deteriorated.

[0089] In Comparative Example 2, lecithin is not used to modify polyphosphate kinase, and the moisturizing and repairing properties of the composition with the addition of natural NMF moisturizing factor are poor. Lecithin can effectively encapsulate polyphosphate kinase, thereby promoting the permeability and stability of polyphosphate kinase. However, polyphosphate kinase lacking lecithin is difficult to play a role in promoting the synthesis of APT due to its large molecular weight. Therefore, the moisturizing and repairing properties of the composition with the addition of natural NMF moisturizing factor deteriorate.

[0090] In Comparative Example 3, no sponge extract is added, and the moisturizing and repairing properties of the composition with natural NMF moisturizing factor are poor. The sponge extract is rich in ceramide compounds and inorganic polyphosphates, and polyphosphate kinase can use inorganic polyphosphate as a substrate to synthesize ATP. ATP is the main form of cellular energy, so it can further enhance the effect of polyphosphate kinase synthesis, thereby achieving the repair function. Therefore, the moisturizing and repairing properties of the composition with natural NMF moisturizing factor added are poor without the sponge extract.

[0091] In summary, liposomal enzyme is prepared by polyphosphate kinase and lecithin, and the synergistic effect of polyphosphate kinase and sponge extract is utilized to enhance the metabolic capacity of cells and achieve the ability to repair damaged skin tissue. Lecithin is combined to improve the effect and stability of polyphosphate kinase, and at the same time, other moisturizing components are assisted, which can not only effectively moisturize, but also repair the cell barrier, making the skin healthier.

[0092] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments, and the above embodiments and descriptions are only preferred examples of the present invention, and are not intended to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, and these changes and improvements all fall within the scope of the present invention to be protected.

Claims

1. A method for preparing a composition containing a natural NMF moisturizing factor, characterized in that: The steps include: S1.1, weigh the following raw materials in parts by weight: 10-12 parts by weight of liposome enzyme, 21-25 parts by weight of hyaluronic acid, 4-8 parts by weight of urea, 1-3 parts by weight of glyceryl glucoside, 1-5 parts by weight of sodium lactate, 5-7 parts by weight of neotetraose, 2-4 parts by weight of fucose, 1-3 parts by weight of grape seed oil, 1-5 parts by weight of argan oil, 0.4-0.8 parts by weight of stearyl alcohol, 5-9 parts by weight of sponge extract, 2-4 parts by weight of resurrection grass extract, 8-12 parts by weight of longan kernel extract, 3-5 parts by weight of blackberry leaf extract, 0.5-0.7 parts by weight of horsetail extract and 50-70 parts by weight of glacial water; The liposome enzyme is prepared by mixing polyphosphokinase and lecithin in a mass ratio of 1:10-15; S1.2, heat the glacier water to 75-85℃, add hyaluronic acid, urea, glyceryl glucoside, sodium lactate, neoagarnet, fucus algae, sponge extract, resurrection grass extract, longan kernel extract, blackberry leaf extract and horsetail extract in sequence while stirring, and continue stirring until all ingredients are completely dissolved; S1.

3. Heat grape seed oil and argan oil to 75-85°C, then add stearyl alcohol, stir thoroughly with a magnetic stirrer, then cool to 30-45°C, add liposome enzyme, and finally cool to room temperature to obtain a composition with added natural NMF moisturizing factor.

2. The method for preparing a composition containing natural NMF moisturizing factor according to claim 1, characterized in that: The preparation steps of the liposome enzyme are specifically as follows: Dissolve lecithin and cholesterol in a mixture of chloroform and methanol to obtain an organic solution, stir thoroughly to ensure that the phospholipids are completely dissolved, transfer the organic solution in which the lecithin and cholesterol are dissolved to a round-bottom flask, use a rotary evaporator to perform rotary evaporation at 35-40°C to form a uniform dry phospholipid film, add buffer a preheated to the same temperature to the dry phospholipid film, oscillate to fully hydrate the phospholipid film to form a liposome suspension, the oscillation frequency is 100-200 times / min, then add polyphosphate kinase to the liposome suspension, perform ultrasonic treatment to encapsulate the polyphosphate kinase inside the liposome, put the liposome suspension containing the polyphosphate kinase into a dialysis bag, place it in buffer b for dialysis, and obtain a modified product liposome enzyme.

3. The method for preparing the composition containing natural NMF moisturizing factor according to claim 2, characterized in that: The mass ratio of lecithin to cholesterol is 10:0.8-1.

3.

4. The method for preparing the composition containing natural NMF moisturizing factor according to claim 2, characterized in that: In the mixed solution of chloroform and methanol, the volume ratio of chloroform to methanol is 2-3:

1.

5. The method for preparing the composition containing natural NMF moisturizing factor according to claim 2, characterized in that: The concentration of the lecithin in the mixed solution of chloroform and methanol is 5-7 mg / mL.

6. The method for preparing the composition containing natural NMF moisturizing factor according to claim 2, characterized in that: The buffer solution a and the buffer solution b are both phosphate buffer solutions, and the mass ratio of the buffer solution to the phospholipid film is 1:11-13.

7. The method for preparing the composition containing natural NMF moisturizing factor according to claim 2, characterized in that: The ultrasonic treatment has an ultrasonic time of 20-30 minutes and a power setting of 20-50W.

8. The method for preparing the composition containing natural NMF moisturizing factor according to claim 2, characterized in that: The molecular cut-off of the dialysis bag is 10-30 kDa.

9. The method for preparing the composition containing natural NMF moisturizing factor according to claim 2, characterized in that: The dialysis time is 12-24 hours, and the dialysis temperature is controlled at 3-5°C.

10. The method for preparing the composition containing natural NMF moisturizing factor according to claim 6, characterized in that: The pH value of the phosphate buffer is 7.0-7.5, and the buffer is replaced every 4-6 hours.

Images