Anti-skin aging lipidosome as well as preparation method and application thereof

Abstract

The invention discloses an anti-skin aging liposome as well as a preparation method and application thereof, and relates to the technical field of cosmetics. The skin aging resisting liposome is prepared and formed by carrying out liposome self-assembly on the following raw materials in parts by mass: 14 to 18 parts of a retinol functional substance, 1 to 3 parts of epicatechin-3-gallate, 1 to 2 parts of glutathione, 4 to 8 parts of lipid, 2 to 16 parts of an emulsifier, 1 to 10 parts of a co-emulsifier and 1 to 5 parts of a stabilizer, the retinol functional substance is prepared from 5 to 9 parts of retinol and 7 to 11 parts of hydroxyl pinacolone retinoate, and the proportion of the retinol is not greater than that of the hydroxyl pinacolone retinoate. The anti-skin aging liposome disclosed by the invention has multi-dimensional synergistic effects of promoting skin cell proliferation and differentiation, reducing skin inflammation stimulation, improving skin oxidation resistance, promoting collagen generation and inhibiting MMPs from degrading collagen, and can achieve an excellent anti-skin aging effect.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to an anti-skin aging liposome, a preparation method and application thereof. Background Art

[0002] Skin photoaging is caused by excessive UV stimulation of matrix metalloproteinase-1 (MMP-1) in the body, which leads to excessive degradation of collagen in the papillary dermis, resulting in wrinkles, decreased elasticity / loosening of the skin, and other symptoms. Studies have shown that retinol can inhibit the activity of MMP-1 after excessive UV stimulation, and is expected to be used to prevent skin photoaging. However, its high instability in light, oxygen, temperature and pH value and obvious skin irritation limit its direct application in cosmetics. Therefore, the development of retinol cosmetics that can inhibit MMP-1 has become a hot topic.

[0003] At present, in order to solve the defect that retinol cannot be directly used in cosmetics, relevant technologies disclose that retinol and its derivatives can be encapsulated by encapsulation technology. For example, the technical scheme with announcement number CN 113975189B discloses a low-irritation retinol encapsulation carrier, which reduces the irritation and instability of retinol substances to the skin by combining retinol substances with plant fermentation products (sophora japonica bud fermentation liquid, chamomile fermentation liquid, Centella asiatica fermentation liquid).

[0004] However, the retinol-encapsulating carriers mentioned above still have some shortcomings. For example, although the added plant fermentation products can promote the release of retinol-like substances, the encapsulated substance is only retinol, which inevitably limits the efficacy pathway, and the plant fermentation products are not easy to obtain and are expensive. In addition, the current retinol cosmetics generally achieve anti-aging effects by promoting the production of collagen, and there are few technical reports on the multi-dimensional combined effects of inhibiting matrix metalloproteinase 1. Summary of the invention

[0005] The present invention provides an anti-skin aging liposome and a preparation method and application thereof, thereby effectively solving the technical problems of the existing retinol-encapsulated carriers having a single efficacy pathway and high cost.

[0006] In order to achieve the above technical objectives, the present invention provides the following technical solutions:

[0007] The first aspect of the present invention provides an anti-skin aging liposome, which comprises liposome self-assembly preparation and molding by raw materials comprising the following parts by mass:

[0008] 14-18 parts of retinol-like functional ingredients, 1-3 parts of epicatechin-3-gallate, 1-2 parts of glutathione, 4-8 parts of lipids, 2-16 parts of emulsifiers, 1-10 parts of co-emulsifiers, and 1-5 parts of stabilizers;

[0009] The retinol-based functional ingredient is composed of 5-9 parts of retinol and 7-11 parts of hydroxypinacolone retinoic acid ester, and the proportion of the retinol is no greater than that of the hydroxypinacolone retinoic acid ester.

[0010] According to the disclosure of the first aspect of the present invention, the anti-skin aging liposomes are prepared by liposome self-assembly of raw materials comprising the following parts by mass:

[0011] 16 parts of retinol-like functional ingredients, 1 part of epicatechin-3-gallate, 1 part of glutathione, 4-8 parts of lipids, 2-16 parts of emulsifiers, 1-10 parts of co-emulsifiers, and 1-5 parts of stabilizers;

[0012] The retinol-based functional ingredient is composed of 5-8 parts of retinol and 8-11 parts of hydroxypinacolone retinoic acid ester.

[0013] According to the disclosure of the first aspect of the present invention, 16 parts of retinol-based functional ingredients, 1 part of epicatechin-3-gallate, 1 part of glutathione, 4-8 parts of lipids, 2-16 parts of emulsifiers, 1-10 parts of co-emulsifiers, and 1-5 parts of stabilizers;

[0014] The retinol-based active ingredient is composed of 7-8 parts of retinol and 8-9 parts of hydroxypinacolone retinoic acid ester.

[0015] According to the disclosure of the first aspect of the present invention, the lipid comprises one or more of lecithin, caprylic / capric triglyceride, glyceryl monostearate, butyloctanol salicylate, and acetylated monoglyceride.

[0016] According to the disclosure of the first aspect of the present invention, the emulsifier comprises one or more of polyglyceryl 10 stearate, isopropyl laurate, PEG-8 caprylic / capric glyceride, sucrose stearate, cetearyl olive oil, sorbitan olive oil, laureth-23, PEG-4 olive oil, steareth-21, palmityl alcohol, glyceryl stearate, PEG-75 stearate, steareth-20, cetyl alcohol, cetyl alcohol ether-20, and steareth-20.

[0017] According to the disclosure of the first aspect of the present invention, the co-emulsifier comprises one or more of glycerol, 1,3-propylene glycol, 1,2-pentanediol ethoxydiglycol, olive oil PEG-7 esters, polyglyceryl-3-diisostearate, polyglyceryl-6 isostearate, and polyethylene glycol-400.

[0018] According to the disclosure of the first aspect of the present invention, the stabilizer comprises one or more of 1,3-butylene glycol, hydrogenated olive oil, olive oil, unsaponifiable matter of olive oil, cetyl palmitate, sorbitan palmitate, and sorbitan olivate.

[0019] The second aspect of the present invention also provides a method for preparing the anti-skin aging liposomes of the present invention, which comprises:

[0020] After the formulated amount of lipid is dissolved, the formulated amount of retinol, hydroxypinacolone retinoic acid ester, epicatechin-3-gallate and glutathione are added and mixed by ultrasound to obtain phase A;

[0021] After the formulated amount of lipid is dissolved, the formulated amount of retinol, hydroxypinacolone retinoic acid ester, epicatechin-3-gallate and glutathione are added and mixed by ultrasound to obtain phase A;

[0022] Add the formulated amount of emulsifier, co-emulsifier and stabilizer to 100 parts of water and heat and mix to obtain phase B;

[0023] The phase A is added to the phase B, and after homogenization, the pH is adjusted to 6.5-7 to obtain the anti-skin aging liposome.

[0024] The third aspect of the present invention also provides use of the anti-skin aging liposomes of the present invention described above in the preparation of cosmetics.

[0025] The fourth aspect of the present invention also provides a firming and whitening nanoemulsion, which comprises the following raw materials in parts by weight:

[0026] 0.5-1 part of the anti-skin aging liposome described in the present invention;

[0027] 0.05 parts of sodium hyaluronate, 0.05 parts of ammonium acryloyldimethyl taurate / beheneth-25 methacrylate crosspolymer, 0.10 parts of sodium polyacryloyldimethyl taurate, 0.10 parts of ammonium acryloyldimethyl taurate / VP copolymer, 0.5 parts of water-soluble silicone oil, 0.5 parts of 1,2-hexanediol, and 0.5 parts of p-hydroxyacetophenone;

[0028] And, make up to 50 parts of water.

[0029] The fifth aspect of the present invention also provides a method for preparing the firming and whitening nanoemulsion of the present invention, comprising:

[0030] Heat and mix the formulated amounts of sodium hyaluronate, sodium polyacryloyldimethyl taurate, 1,2-hexanediol, ammonium acryloyldimethyl taurate / beheneth-25 methacrylate crosspolymer, ammonium acryloyldimethyl taurate / VP copolymer and water-soluble silicone oil, add into water and mix well to obtain phase C;

[0031] The anti-skin aging liposome is added into the phase C, and the mixture is stirred and evenly mixed, and p-hydroxyacetophenone is added to obtain the firming and whitening nanoemulsion.

[0032] Compared with the prior art, the beneficial effects of the present invention at least include:

[0033] The anti-skin aging liposomes provided by the present invention encapsulate the contained retinol, hydroxypinacolone retinoic acid ester, epicatechin-3-gallate and glutathione through liposome technology, so that the prepared liposomes have a multi-dimensional synergistic effect of promoting skin cell proliferation and differentiation, reducing skin inflammatory stimulation, improving skin antioxidant capacity, promoting collagen production and inhibiting MMPs from degrading collagen, thereby achieving excellent anti-skin aging effects. Specifically, retinol and hydroxypinacolone retinoic acid ester can promote skin cell proliferation and differentiation, reduce melanin deposition, stimulate collagen synthesis and inhibit MMPs from degrading collagen through the RAR / RXR signaling pathway, effectively reducing skin photoaging. At the same time, adding glutathione and epicatechin-3-gallate for efficacy compounding can activate Nrf2 (nuclear factor E2-related factor 2) and AMPK pathways, upregulate antioxidant enzymes and regulate energy metabolism to improve mitochondrial function, effectively improving the skin's antioxidant protection ability; on the other hand, it can inhibit NF-κB (nuclear factor-κB), reduce the release of inflammatory factors (such as IL-6, TNF-α), reduce skin inflammation and sensitivity, and greatly inhibit the stimulation of skin inflammation; thirdly, it can reduce melanin production and promote the conversion of melanin to melatonin, achieving the effect of whitening the skin. In addition, the stability of retinol-based efficacy substances in various storage environments has been greatly improved, and it is expected to be used in anti-skin aging and whitening cosmetics. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments recorded in the present invention, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0035] Figure 1 A bar graph showing the inhibition rates of different groups of human matrix metalloproteinase 1 provided by the present invention;

[0036] Figure 2 The chicken embryo chorioallantoic membrane images before and after 5 minutes of treatment of different test samples provided by the present invention;

[0037] Figure 3 A bar graph showing the improvement rate of the number of fine lines on the skin of the subjects in different groups provided by the present invention;

[0038] Figure 4 This is a bar graph of the improvement rate of ITA values ​​of the skin of subjects in different groups provided by the present invention. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments described in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0040] In the following description of this specification, the term "and / or" is used to describe the association relationship of associated objects, indicating that there may be three relationships. For example, A and / or B can represent: A exists alone, B exists alone, and A and B exist at the same time. Among them, A and B can be singular or plural; the symbol " / " means "or".

[0041] In the following description of this specification, the term "at least one" means one or more, and "plurality" means two or more. "At least one of the following" or similar expressions refers to any combination of these items, including any combination of single or plural items. For example, "at least one of A, B or C", or "at least one of A, B and C" can mean any one of A, B, C, or A+B, or A+C, or B+C, or A+B+C, where A, B, C can be single or multiple, respectively.

[0042] In the following description of this specification, the order of serial numbers does not mean the order of execution. Some or all of the steps can be executed in parallel or one after the other. The execution order of each process should be determined by its function and internal logic, and does not constitute any limitation on the execution process of this embodiment.

[0043] In the following description of this specification, the numerical range should be understood to also specifically disclose each intermediate value between the upper and lower limits of the range. Any stated value or intermediate value in the stated range and any other stated value or each smaller range between the intermediate values ​​in the range are also included in this embodiment, and the upper and lower limits of the smaller range can be independently included or excluded in the range.

[0044] Unless otherwise specified, the technical / scientific terms used in this specification have the meanings commonly understood by those of ordinary skill in the art. Although this specification only describes preferred materials and methods, any similar or equivalent methods and materials may also be used in specific embodiments or test cases. All documents mentioned in this specification are incorporated by reference to disclose and describe methods and / or materials related to the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0045] Abbreviations of some substances used in the examples of the present invention: retinol (A1); hydroxypinacolone retinoate (HPR); epicatechin-3-gallate 1 part (EGCG); glutathione (GSH); ammonium acryloyldimethyltaurate / beheneth-25 methacrylate crosspolymer (HMB); sodium polyacryloyldimethyltaurate (Silk); ammonium acryloyldimethyltaurate / VP copolymer (AVC).

[0046] The technical solution of the present invention will be further described below in conjunction with specific embodiments.

[0047] Example 1

[0048] This embodiment provides a preparation method of anti-skin aging liposomes, specifically:

[0049] After dissolving 4 parts by mass of lecithin, 7 parts by mass of retinol, 9 parts by mass of hydroxypinacolone retinoic acid ester, 1 part by mass of epicatechin-3-gallate and 1 part by mass of glutathione were added and mixed by sonication to obtain phase A;

[0050] 4 parts by mass of polyglycerol 10 stearate, 1 part by mass of glycerol and 1 part by mass of 1,3-butanediol were heated and mixed, and then added into 100 parts of deionized water and stirred to mix evenly to obtain phase B;

[0051] The phase A was added to the phase B, and homogenized for 3 times at a temperature of 80° C. and a pressure of 100 MPa. The pH was adjusted to 6.5 and stirring was continued for 0.5 h to obtain the anti-skin aging liposomes.

[0052] Example 2

[0053] This embodiment provides a preparation method of anti-skin aging liposomes, specifically:

[0054] After dissolving 5 parts by mass of caprylic / capric triglyceride, 8 parts by mass of retinol, 8 parts by mass of hydroxypinacolone retinoic acid ester, 1 part by mass of epicatechin-3-gallate and 1 part by mass of glutathione were added and mixed by ultrasound to obtain phase A;

[0055] 4 parts by mass of lauric acid isopropyl ester, 3 parts by mass of 1,3-propylene glycol and 2 parts by mass of hydrogenated olive oil were heated and mixed, and then added into 100 parts of deionized water and stirred to mix evenly to obtain phase B;

[0056] The phase A was added to the phase B, and homogenized for 3 times at a temperature of 80° C. and a pressure of 100 MPa. The pH was adjusted to 6.5 and stirring was continued for 0.5 h to obtain the anti-skin aging liposomes.

[0057] Example 3

[0058] This embodiment provides a preparation method of anti-skin aging liposomes, specifically:

[0059] After dissolving 7 parts by mass of butyl octanol salicylate, 5 parts by mass of retinol, 11 parts by mass of hydroxypinacolone retinoic acid ester, 1 part by mass of epicatechin-3-gallate and 1 part by mass of glutathione were added and mixed by sonication to obtain phase A;

[0060] 8 parts by mass of sucrose stearate, 7 parts by mass of olive oil PEG-7 esters and 3 parts by mass of olive oil unsaponifiable matter were heated and mixed, and then added into 100 parts of deionized water and stirred to mix evenly to obtain phase B;

[0061] The phase A was added to the phase B, and homogenized for 3 times at a temperature of 80° C. and a pressure of 100 MPa. The pH was adjusted to 6.5 and stirring was continued for 0.5 h to obtain the anti-skin aging liposomes.

[0062] Example 4

[0063] This embodiment provides a preparation method of anti-skin aging liposomes, specifically:

[0064] After dissolving 4 parts by mass of lecithin, 7 parts by mass of retinol, 7 parts by mass of hydroxypinacolone retinoic acid ester, 1 part by mass of epicatechin-3-gallate and 1 part by mass of glutathione were added and mixed by ultrasound to obtain phase A;

[0065] 4 parts by mass of polyglycerol 10 stearate, 1 part by mass of glycerol and 1 part by mass of 1,3-butanediol were heated and mixed, and then added into 100 parts of deionized water and stirred to mix evenly to obtain phase B;

[0066] The phase A was added to the phase B, and homogenized for 3 times at a temperature of 80° C. and a pressure of 100 MPa. The pH was adjusted to 6.5 and stirring was continued for 0.5 h to obtain the anti-skin aging liposomes.

[0067] Example 5

[0068] This embodiment provides a preparation method of anti-skin aging liposomes, specifically:

[0069] After dissolving 4 parts by mass of lecithin, 9 parts by mass of retinol, 9 parts by mass of hydroxypinacolone retinoic acid ester, 1 part by mass of epicatechin-3-gallate and 1 part by mass of glutathione were added and mixed by ultrasound to obtain phase A;

[0070] 4 parts by mass of polyglycerol 10 stearate, 1 part by mass of glycerol and 1 part by mass of 1,3-butanediol were heated and mixed, and then added into 100 parts of deionized water and stirred to mix evenly to obtain phase B;

[0071] The phase A was added to the phase B, and homogenized for 3 times at a temperature of 80° C. and a pressure of 100 MPa. The pH was adjusted to 6.5 and stirring was continued for 0.5 h to obtain the anti-skin aging liposomes.

[0072] Comparative Example 1

[0073] This comparative example provides a preparation method for a double A composition liposome, specifically:

[0074] After dissolving 4 parts by weight of lecithin, 7 parts by weight of retinol and 9 parts by weight of hydroxypinacolone retinoic acid ester were added and mixed by ultrasound to obtain phase A;

[0075] 4 parts by mass of polyglycerol 10 stearate, 1 part by mass of glycerol and 1 part by mass of 1,3-butanediol were heated and mixed, and then added into 100 parts of deionized water and stirred to mix evenly to obtain phase B;

[0076] The phase A was added to the phase B, and homogenized for 3 times at a temperature of 80° C. and a pressure of 100 MPa. The pH was adjusted to 6.5 and stirring was continued for 0.5 h to obtain the anti-skin aging liposomes.

[0077] Comparative Example 2

[0078] This comparative example provides a preparation method for a double A composition liposome, specifically:

[0079] After dissolving 4 parts by weight of lecithin, 7 parts by weight of retinol, 9 parts by weight of hydroxypinacolone retinoic acid ester and 1 part by weight of glutathione were added and mixed by ultrasound to obtain phase A;

[0080] 4 parts by mass of polyglycerol 10 stearate, 1 part by mass of glycerol and 1 part by mass of 1,3-butanediol were heated and mixed, and then added into 100 parts of deionized water and stirred to mix evenly to obtain phase B;

[0081] The phase A was added to the phase B, and homogenized for 3 times at a temperature of 80° C. and a pressure of 100 MPa. The pH was adjusted to 6.5 and stirring was continued for 0.5 h to obtain the anti-skin aging liposomes.

[0082] Comparative Example 3

[0083] This comparative example provides a preparation method for a double A composition liposome, specifically:

[0084] After dissolving 4 parts by weight of lecithin, 7 parts by weight of retinol, 9 parts by weight of hydroxypinacolone retinoic acid ester and 1 part by weight of epicatechin-3-gallate were added and mixed by ultrasound to obtain phase A;

[0085] 4 parts by mass of polyglycerol 10 stearate, 1 part by mass of glycerol and 1 part by mass of 1,3-butanediol were heated and mixed, and then added into 100 parts of deionized water and stirred to mix evenly to obtain phase B;

[0086] The phase A was added to the phase B, and homogenized for 3 times at a temperature of 80° C. and a pressure of 100 MPa. The pH was adjusted to 6.5 and stirring was continued for 0.5 h to obtain the anti-skin aging liposomes.

[0087] Comparative Example 4

[0088] After dissolving 4 parts by mass of lecithin, 5 parts by mass of retinol, 7 parts by mass of hydroxypinacolone retinoic acid ester, 1 part by mass of epicatechin-3-gallate and 1 part by mass of glutathione were added and mixed by ultrasound to obtain phase A;

[0089] 4 parts by mass of polyglycerol 10 stearate, 1 part by mass of glycerol and 1 part by mass of 1,3-butanediol were heated and mixed, and then added into 100 parts of deionized water and stirred to mix evenly to obtain phase B;

[0090] The phase A was added to the phase B, and homogenized for 3 times at a temperature of 80° C. and a pressure of 100 MPa. The pH was adjusted to 6.5 and stirring was continued for 0.5 h to obtain the anti-skin aging liposomes.

[0091] Comparative Example 5

[0092] After dissolving 4 parts by mass of lecithin, 9 parts by mass of retinol, 11 parts by mass of hydroxypinacolone retinoic acid ester, 1 part by mass of epicatechin-3-gallate and 1 part by mass of glutathione were added and mixed by ultrasound to obtain phase A;

[0093] 4 parts by mass of polyglycerol 10 stearate, 1 part by mass of glycerol and 1 part by mass of 1,3-butanediol were heated and mixed, and then added into 100 parts of deionized water and stirred to mix evenly to obtain phase B;

[0094] The phase A was added to the phase B, and homogenized for 3 times at a temperature of 80° C. and a pressure of 100 MPa. The pH was adjusted to 6.5 and stirring was continued for 0.5 h to obtain the anti-skin aging liposomes.

[0095] In order to illustrate the performance of the anti-skin aging liposomes prepared by the present invention, the liposomes prepared by the examples and comparative examples were tested for their performance, specifically:

[0096] 1. Particle size and stability test

[0097] The liposome samples prepared in Example 1 and Comparative Examples 1-5 were placed in sealed containers at -18°C, 4°C, room temperature and 45°C for 180 days. The appearance of the liposome samples was checked at room temperature for precipitation or discoloration in the initial state, 90 days and 180 days, and the particle size and potential of the liposome samples were tested.

[0098] Table 1: Particle size, Zeta potential and appearance test results of liposome samples

[0099]

[0100]

[0101] According to Table 1, when the amount of retinol-based functional substance (retinol + hydroxypinacolone retinoic acid ester) added is less than 20, the particle size, potential and appearance of the liposome emulsion remain relatively stable; when the amount of retinol-based functional substance added reaches 20, the liposome emulsion has a significant increase in particle size, a decrease in potential and a darkening of color, indicating that the amount of retinol-based functional substance added significantly affects the stability of the liposome. Therefore, the present invention preferably adds a retinol-based functional substance in an amount of less than or equal to 18 to ensure that the prepared liposome has a good storage effect.

[0102] 2. Determination of active substance retention rate

[0103] The liposomes prepared in Example 1 and Comparative Examples 4-5 were placed in sealed containers at -18°C, 4°C, room temperature and 45°C for 180 days, and the concentration of active substances in the liposome emulsions was detected by liquid phase at the initial state, 90 days and 180 days later.

[0104] Table 2: Test results of A1 and HPR content in liposome samples

[0105]

[0106] According to Table 2, when the amount of retinol-based functional substance (retinol + hydroxypinacolone retinoic acid ester) added is less than 20, the concentration of retinol and hydroxypinacolone retinoic acid ester entrapped in the liposome is relatively stable; when the amount of retinol-based functional substance added reaches 20, the concentration of retinol and hydroxypinacolone retinoic acid ester entrapped in the liposome decreases significantly, and the decreasing trend accelerates with the increase of storage temperature. Therefore, the present invention preferably adds an amount of retinol-based functional substance less than or equal to 18 to ensure that the prepared liposome has good storage stability.

[0107] 3. Anti-cell aging (matrix metalloproteinase) experiment

[0108] HDF cells in logarithmic growth phase (cell density 2×10 5 ~5×10 5 / mL) were inoculated in a 24-well plate, 1mL per well, and incubated for 24h. After 24h, the supernatant was aspirated and 1mL of the following samples were added respectively: ① Blank group: DMEM complete medium (10% FBS; 1% double antibody); ② Experimental group: test substance (1%) + DMEM complete medium. After acting on human skin fibroblasts for 24h, the supernatant was aspirated into a centrifuge tube with a pipette, centrifuged at 1000r / min for 5min, the precipitate was removed and the supernatant was aspirated, and the amount of human matrix metalloproteinase 1 in the supernatant was detected using a human matrix metalloproteinase 1 ELISA kit (Shanghai ELISA Biological) (the detection method was strictly in accordance with the instructions on the detection kit), and the human matrix metalloproteinase 1 inhibition rate was calculated according to the following formula, and the result was Figure 1 Wherein, the test object is the liposome prepared by the embodiment and the comparative example.

[0109]

[0110] according to Figure 1 It can be seen that the liposome emulsions prepared in Examples 1-5 have significantly better inhibitory effects on human matrix metalloproteinase 1 than Comparative Examples 1-5, indicating that the combination of retinol-based functional substances, epicatechin-3-gallate and glutathione has a synergistic inhibitory effect on human matrix metalloproteinase 1, and the amount of retinol-based functional substances added is preferably 14-18. In particular, the liposome emulsions prepared in Examples 1-3 have significantly better inhibitory effects on human matrix metalloproteinase 1 than Examples 4-5, so the amount of retinol-based functional substances added is more preferably 16.

[0111] 4. The anti-skin aging liposomes prepared in Example 1 were prepared into a firming and whitening nanoemulsion, and the performance of the firming and whitening nanoemulsion was tested, specifically:

[0112] 0.05 parts by mass of sodium hyaluronate, 0.05 parts by mass of ammonium acryloyldimethyltaurate / beheneth-25 methacrylate crosspolymer, 0.10 parts by mass of sodium polyacryloyldimethyltaurate, 0.10 parts by mass of ammonium acryloyldimethyltaurate / VP copolymer, 0.5 parts by mass of 1,2-hexanediol and 0.5 parts by mass of water-soluble silicone oil were heated and mixed, and then added into water and mixed evenly to obtain phase C;

[0113] 0.1, 0.2, 0.5, 1.0, 1.2 and 1.5 parts by weight of the anti-skin aging liposomes of Example 1 were added to the phase C respectively, stirred and mixed evenly, and 0.5 parts by weight of p-hydroxyacetophenone was added to prepare firming and whitening nanoemulsions with different efficacy concentrations in turn.

[0114] 4.1 Stability test of firming and whitening nanoemulsion

[0115] The firming and whitening nanoemulsion was placed in sealed containers at -18°C, 4°C, room temperature and 45°C for 180 days. The color state changes of the firming and whitening nanoemulsion were observed at the initial state, 90 days and 180 days later, and the concentration of retinol-like functional substances in the firming and whitening nanoemulsion was detected by liquid phase. The results are shown in Table 3.

[0116] Table 3: Appearance of the firming and whitening nanoemulsion samples and test results of the content of retinol-like functional ingredients

[0117]

[0118]

[0119] According to Table 3, at room temperature and below, the shape of the samples stored by the firming and whitening nanoemulsion remains good without obvious changes, indicating that the firming and whitening nanoemulsion has good low-temperature stability; however, when stored at 45°C, the addition of anti-skin aging liposomes exceeding 1% will cause the active ingredient content of the firming and whitening nanoemulsion to decrease significantly over time. Therefore, the addition amount of anti-skin aging liposomes contained in the firming and whitening nanoemulsion is preferably less than or equal to 1%.

[0120] 4.2 Irritation test:

[0121] The firming and whitening nanoemulsion was subjected to a chicken embryo irritation test to evaluate the irritation. The specific test method was: after removing the eggshell and inner membrane of the air chamber of SPF eggs incubated for 9-12 days, 0.3 mL of the sample to be tested was slowly dripped into the chorioallantoic membrane (CAM) with a pipette, and the reaction changes of the CAM within 5 minutes were observed and photographed and recorded. The results were Figure 2 As shown, the blank control was 0.9% normal saline; the positive control was 0.4% sodium hydroxide; and the experimental group was the firming and whitening nanoemulsion of test samples 1-6.

[0122]

[0123] 4.2-2: Evaluation of results by endpoint scoring method

[0124] End point scoring Irritation classification Rating ≤2 No / mild irritation <Rating<4 Moderate irritation Rating ≥4 Strong irritant / corrosive

[0125] 4.2-3: Evaluation results

[0126]

[0127] according to Figure 2The evaluation results show that after the firming and whitening nanoemulsions of test samples 1-4 acted on CAM for 5 minutes, there was no bleeding, no coagulation, and no vascular dissolution; after the firming and whitening nanoemulsions of test samples 1-4 acted on CAM for 5 minutes, there was slight bleeding, that is, the firming and whitening nanoemulsions of test samples 1-4 were non-irritating, and the firming and whitening nanoemulsions of test samples 5-6 were slightly irritating. Therefore, the addition amount of the anti-skin aging liposome is preferably less than or equal to 1.

[0128] 4.3 Human efficacy test:

[0129] Based on the above results, the effect of the added amount of anti-skin aging liposomes on the skin improvement effect was investigated.

[0130] Subjects: 40 people in total, 10 people in each group, numbered 1-10. The subjects used the product on their face twice a day, 1 mL each time, evenly applied on the face, for 28 consecutive days. The subjects were healthy volunteers with no history of cosmetic allergies.

[0131] Visia skin detector was used to detect the number of fine lines and ITA value changes of the subjects. The test results are shown in Table 7. Figure 3 , Figure 4 shown.

[0132] Table 4.3: Changes in the number of fine lines and ITA values ​​of the subjects

[0133]

[0134] according to Figure 3 , Figure 4 As shown in Table 4.3, the multifunctional essences prepared in Test Examples 1 to 4 were applied to the face of the subjects twice a day for 28 consecutive days. The improvement rate of the number of fine lines and the improvement rate of the ITA value in Test Example 4 were the highest. Therefore, the addition amount of the anti-skin aging liposome is preferably 0.5-1 part.

[0135] The various embodiments in this specification are described in a progressive manner, and the same or similar parts between the various embodiments can be referenced to each other, and each embodiment focuses on the differences from other embodiments.

[0136] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, it should be understood by those skilled in the art that the technical solutions recorded in the aforementioned embodiments can still be modified, or some or all of the technical features therein can be replaced by equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the present invention.

Claims

1. An anti-skin aging liposome, characterized in that: The method comprises preparing liposomes by self-assembly from the following raw materials in parts by weight: 14-18 parts of retinol-like functional ingredients, 1-3 parts of epicatechin-3-gallate, 1-2 parts of glutathione, 4-8 parts of lipids, 2-16 parts of emulsifiers, 1-10 parts of co-emulsifiers, and 1-5 parts of stabilizers; The retinol-based functional substance is composed of 5-9 parts of retinol and 7-11 parts of hydroxypinacolone retinoic acid ester, and the proportion of the retinol is no greater than that of the hydroxypinacolone retinoic acid ester.

2. The anti-skin aging liposome according to claim 1, characterized in that: The method comprises preparing liposomes by self-assembly from the following raw materials in parts by weight: 16 parts of retinol-like functional ingredients, 1 part of epicatechin-3-gallate, 1 part of glutathione, 4-8 parts of lipids, 2-16 parts of emulsifiers, 1-10 parts of co-emulsifiers, and 1-5 parts of stabilizers; The retinol-based functional ingredient is composed of 5-8 parts of retinol and 8-11 parts of hydroxypinacolone retinoic acid ester.

3. The anti-skin aging liposome according to claim 2, characterized in that: The method comprises preparing liposomes by self-assembly from the following raw materials in parts by weight: 16 parts of retinol-like functional ingredients, 1 part of epicatechin-3-gallate, 1 part of glutathione, 4-8 parts of lipids, 2-16 parts of emulsifiers, 1-10 parts of co-emulsifiers, and 1-5 parts of stabilizers; Wherein, the retinol-based functional substance is composed of 7-8 parts of retinol and 8-9 parts of hydroxypinacolone retinoic acid ester.

4. The anti-skin aging liposome according to any one of claims 1 to 3, characterized in that: The lipid comprises one or more of lecithin, caprylic / capric triglyceride, glyceryl monostearate, butyloctanol salicylate, and acetylated monoglyceride.

5. The anti-skin aging liposome according to any one of claims 1 to 3, characterized in that: The emulsifier comprises one or more of polyglyceryl 10 stearate, isopropyl laurate, PEG-8 caprylic / capric glyceride, sucrose stearate, cetearyl olive oil ester, sorbitan olive oil ester, laureth-23, PEG-4 olive oil ester, steareth-21, palmityl alcohol, glyceryl stearate, PEG-75 stearate, steareth-20, cetyl alcohol, cetyl alcohol ether-20, and steareth-20; The auxiliary emulsifier comprises one or more of glycerol, 1,3-propylene glycol, 1,2-pentanediol ethoxydiglycol, olive oil PEG-7 esters, polyglyceryl-3-diisostearate, polyglyceryl-6 isostearate, and polyethylene glycol-400.

6. The anti-skin aging liposome according to any one of claims 1 to 3, characterized in that: The stabilizer comprises one or more of 1,3-butylene glycol, hydrogenated olive oil, olive oil, unsaponifiable matter of olive oil, cetyl palmitate, sorbitan palmitate, and sorbitan olivate.

7. A method for preparing the anti-skin aging liposome according to any one of claims 1 to 6, characterized in that: Include: After the formulated amount of lipid is dissolved, the formulated amount of retinol, hydroxypinacolone retinoic acid ester, epicatechin-3-gallate and glutathione are added and mixed by ultrasound to obtain phase A; Add the formulated amount of emulsifier, co-emulsifier and stabilizer to 100 parts of water and heat and mix to obtain phase B; The phase A is added to the phase B, and after homogenization, the pH is adjusted to 6.5-7 to obtain the anti-skin aging liposome.

8. Use of the anti-skin aging liposome according to any one of claims 1 to 6 in preparing cosmetics.

9. A firming and whitening nanoemulsion, characterized in that: Contains the following raw materials by weight: 0.5-1 part of the anti-skin aging liposome according to any one of claims 1-6; 0.05 parts of sodium hyaluronate, 0.05 parts of ammonium acryloyldimethyl taurate / beheneth-25 methacrylate crosspolymer, 0.10 parts of sodium polyacryloyldimethyl taurate, 0.10 parts of ammonium acryloyldimethyl taurate / VP copolymer, 0.5 parts of water-soluble silicone oil, 0.5 parts of 1,2-hexanediol, and 0.5 parts of p-hydroxyacetophenone; And, make up to 50 parts of water.

10. A method for preparing the firming and whitening nanoemulsion according to claim 9, characterized in that: Include: Heat and mix the formulated amounts of sodium hyaluronate, sodium polyacryloyldimethyl taurate, 1,2-hexanediol, ammonium acryloyldimethyl taurate / beheneth-25 methacrylate crosspolymer, ammonium acryloyldimethyl taurate / VP copolymer and water-soluble silicone oil, add into water and mix well to obtain phase C; The anti-skin aging liposome is added into the phase C, and the mixture is stirred and evenly mixed, and p-hydroxyacetophenone is added to obtain the firming and whitening nanoemulsion.

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