Natural tightening, anti-wrinkle and skin-brightening plant essential oil as well as preparation method and application thereof

Abstract

The invention discloses natural tightening, anti-wrinkle and skin-brightening plant essential oil and a preparation method and application thereof, and belongs to the field of skin care products. The essential oil comprises frankincense oil, sandalwood oil, lavender oil, myrrh oil, Italian helichrysum flower oil, rock rose oil, rosa damascena flower oil, vetiver root oil and marine biosterol. The marine organism sterol is formed by mixing ascidea pedicularis sterol and sarcophyton ascophyllum sterol. The plant essential oil can be applied to skin care products such as water aqua, facial masks, essence, emulsion, face cream and liquid foundation. According to the invention, a unique essential oil formula is adopted, so that the product has prominent comprehensive effects on wrinkle resistance and skin brightening, does not contain any chemical component, accords with relevant provisions of Cosmetic Safety Technical Specifications, and has high safety; according to the anti-wrinkle skin-brightening essential oil disclosed by the invention, the marine organism sterol prepared from the ascidosterols and the sarcosterols is added into the plant essential oil, so that the anti-wrinkle skin-brightening essential oil has the effects of resisting oxidation and moisturizing, and also can improve the permeation and absorption of the essential oil, thereby further improving the anti-wrinkle skin-brightening effect.

Description

Technical Field

[0001] The invention relates to the technical field of skin care product preparation, and in particular to a natural firming, anti-wrinkle and skin-lightening plant essential oil and a preparation method and application thereof. Background Art

[0002] Plant essential oils have many advantages in skin care, including improving skin permeability, lubricating skin and maintaining elasticity, promoting metabolism and detoxification, aromatherapy effect and psychological relief, enhancing skin protection, improving makeup adhesion, and refreshing and natural feeling. These advantages make plant essential oils an ideal choice for many people in daily skin care.

[0003] For example, the patent document with the authorization publication number CN115487096B discloses a plant essential oil composition and application with the effects of removing dark circles, moisturizing and repairing, which has the effects of removing dark circles, moisturizing, repairing and restoring elasticity. For another example, the patent document with the authorization publication number CN104490704B discloses a plant essential oil skin care product, which has the effects of moisturizing the skin and shrinking pores.

[0004] However, the existing skin care products based on these plant essential oil compositions on the market have average anti-wrinkle and skin lightening effects when used. Therefore, it is urgent to optimize the formula of these essential oil compositions. Summary of the invention

[0005] The purpose of the present invention is to provide a natural firming, anti-wrinkle and skin-lightening plant essential oil and a preparation method and application thereof, so as to enhance the anti-wrinkle and skin-lightening effects thereof.

[0006] The present invention achieves the above-mentioned purpose through the following technical solutions: A natural firming, anti-wrinkle and skin-lightening plant essential oil, wherein the raw materials of the plant essential oil include, by weight percentage: Frankincense oil 30.0-40.0%; Sandalwood oil 15.0-20.0%; Lavender oil 10.0-15.0%; Myrrh oil 8.0-12.0%; Italian Immortelle flower oil 12.0-18.0%; Cistus oil 0.5-1.5%; Rosa damascena flower oil 5.0-10.0%; Vetiver root oil 0.5-1.5%; Marine sterols 0.1-0.5%.

[0007] A further improvement is that, by weight percentage, the raw materials of the plant essential oil include: Frankincense oil 35.0%; Sandalwood oil 17.5%; Lavender oil 12.5%; Myrrh oil 10.0%; Italian Immortelle flower oil 15.0%; Cistus oil 1.0%; Rosa damascena flower oil 8.0%; Vetiver root oil 0.98%; Marine sterols 0.02%.

[0008] A further improvement is that the marine sterol is a mixture of ascidian sterol and saxifragales sterol.

[0009] A further improvement is that the weight ratio of the sterol to the sterol is 1:3.

[0010] The present invention also provides a method for preparing the natural firming, anti-wrinkle and skin-lightening plant essential oil, the steps comprising: S1. Weigh frankincense oil, sandalwood oil, lavender oil, myrrh oil, Italian immortelle oil, rock rose oil, turkish rose oil and vetiver root oil according to weight ratio and place them in a container and mix until homogeneous to obtain a mixed essential oil; S2, preparation of marine sterols; S3. Add the marine sterols to the mixed essential oils according to the weight ratio, and mix evenly again to obtain the product.

[0011] A further improvement is that in step S1, the specific operation of mixing until homogeneous is: stirring and mixing at a rate of 150-300 r / min for 2-4 hours under heating conditions of 35-40°C.

[0012] A further improvement is that the specific operation of step S2 is: S2-1, take fresh stalked sea squirt, wash and dry it, crush it into particles with a particle size of ≤0.2mm, add the particles to a 90% ethanol solution for leaching, filter and take the filtrate after leaching, concentrate it under reduced pressure to obtain a primary extract, then extract the primary extract with ethyl acetate, and then concentrate it under reduced pressure to obtain a secondary extract, then add the secondary extract to a 90% methanol solution, extract it with n-hexane, and then concentrate it under reduced pressure to obtain a n-hexane extract, perform normal pressure silica gel column chromatography on the n-hexane extract, perform gradient elution with chloroform / methanol eluent, and the gradient volume ratio varies from 100:0 to 50:50, monitor by TLC, combine the same parts and concentrate them, and then perform Sephadex LH-20 gel column chromatography, the eluent is methanol:chloroform=1:1, and two components are obtained, and the colorless needle crystal component is taken, which is stalked sea squirt sterol; S2-2, take fresh soft coral, wash and dry it, crush it into particles with a particle size of ≤0.2mm, add the particles to an ethanol solution with a concentration of 90%, extract it, filter and take the filtrate after extraction, decompress and concentrate it to an extract, distribute the extract with ethyl acetate / water, take the ethyl acetate extract and decompress and concentrate it to obtain an ethyl acetate extract, and then perform column chromatography on the extract with silica gel H, perform gradient elution with petroleum ether / ethyl acetate eluent of gradually increasing polarity, and obtain two components in sequence from the eluent with a volume ratio of petroleum ether: ethyl acetate = 20:80, and take the colorless columnar crystal component therein, which is the soft coral sterol; S2-3, taking the sterol of sclerotinia and the sterol of soft coral of phytohormone and mixing them evenly according to the weight ratio, so as to obtain marine sterol.

[0013] A further improvement is that the specific operation of mixing uniformly is: stirring and mixing at a rate of 250-350 r / min for 0.2-1 h under heating conditions of 40-45°C.

[0014] The present invention also provides an application of the natural firming, anti-wrinkle and skin-lightening plant essential oil in skin care products.

[0015] A further improvement is that the skin care product is one of an aqueous solution, a facial mask, an essence, an emulsion, a facial cream, and a liquid foundation. Of course, the plant essential oil can also be diluted and used directly.

[0016] The beneficial effects of the present invention are: (1) The present invention adopts a unique essential oil formula, which makes the product have an outstanding comprehensive effect in anti-wrinkle and skin lightening, and does not contain any chemical ingredients. It complies with the relevant provisions of the "Technical Specifications for Safety of Cosmetics" and is highly safe; (2) The present invention adds marine sterols made from sterols of sclerotin and sterols of soft coral to the plant essential oils, which have antioxidant and moisturizing effects and can also enhance the penetration and absorption of essential oils, thereby further enhancing the anti-wrinkle and skin-lightening effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 The wrinkle number result diagram after using the essential oil samples of each embodiment and comparative example; Figure 2 The wrinkle depth results after using the essential oil samples of each embodiment and comparative example; Figure 3 The wrinkle area results after using the essential oil samples of each embodiment and comparative example; Figure 4 The figure is a graph showing the wrinkle length results after using the essential oil samples of each embodiment and comparative example. DETAILED DESCRIPTION

[0018] The present application is further described in detail below in conjunction with the accompanying drawings. It is necessary to point out here that the following specific implementation methods are only used to further illustrate the present application and cannot be understood as limiting the scope of protection of the present application. Technical personnel in this field can make some non-essential improvements and adjustments to the present application based on the above application content.

[0019] 1. Main instruments and materials Frankincense (BOSWELLIA CARTERII) oil: The main components are α-pinene, limonene, and α-thujaene. Frankincense essential oil has the effect of whitening and lightening spots, and can be used with other essential oils, such as cinnamon and rose essential oils, to lighten spots and remove scars.

[0020] Sandalwood (SANTALUM ALBUM) oil: The main components are α- and β-santalene, santalene, santalene ketone, α-santalene alcohol, and santalone. Sandalwood essential oil can adjust the oil secretion of the skin in terms of skin care, and is suitable for oily and dry skin. It is a mild astringent that can help whiten and lighten spots.

[0021] Lavender (LAVANDULA ANGUSTIFOLIA) oil: The main ingredients are linalool, linalyl acetate, borneol, geraniol, geraniol, lavandin, linalool, limonene, eugenol, coumarin, and pine terpenes. Lavender essential oil has a soothing and calming effect, helps to lighten spots, and it can whiten and lighten spots by inhibiting tyrosinase activity.

[0022] Myrrh (COMMIPHORA MYRRHA) oil: The main components are furanoeucalyptadiene, curcumene, β-cadinene, β-elemene, linderene, and geranylene. Myrrh essential oil can promote blood circulation and remove blood stasis, can treat pigmentation in any part of the body, and help whiten and lighten spots.

[0023] HELICHRYSUM ITALICUM flower oil: The main ingredients are neryl acetate, α-pinene, γ-curcumene, italone I and II. Helichrysum italicum flower oil has the effects of anti-oxidation, promoting collagen production, repairing damaged skin, and soothing sensitive skin.

[0024] Cistus Ladaniferus Oil: The main ingredients are α-pinene, borneol, borneol acetate, sabinene, labdanol, chlorophyll, oleophyllo, and limonene. Cistus Ladaniferus Oil can be used to deeply purify the skin, cleansing it from harmful impurities such as toxins, pollution, and free radicals, leaving the skin radiant, smooth, and vibrant.

[0025] Rosa damascena flower oil: The main ingredients are citronellol, geraniol, nerol, linalool, geraniol acetate, and phenylethyl alcohol. Rosa damascena flower oil can effectively inhibit the deposition of melanin, decompose melanin to make the skin white and smooth, and effectively lighten spots and freckles.

[0026] Vetivertia zizanioides root oil: The main ingredients are custard, nootkatol, β-vetivertene, β-vetivertene, α and β-vetivertone, and vetivertol. Vetivertia zizanioides root oil can help the skin repair itself, moisturize and resist wrinkles, and inhibit the activity of tyrosinase, fade spots, brighten the skin, and improve skin sagging and fine lines.

[0027] Biological sources of sterols: Fresh Styela clava: collected from the scallop culture area in the coastal aquaculture area of ​​Bohai Sea in Shandong Province; Sarcophyton crassocaule: collected from the waters of Sanya, Hainan, China.

[0028] Testing instruments: Primos skin wrinkle analysis system (LMI Technologies Gmbh Canfield, Canada); Visia-CR (Canfield); Image-Pro comprehensive skin analysis software (Media Cybernetics).

[0029] 2. Implementation of the experiment

[0030] Embodiment 1 A natural firming, anti-wrinkle and skin-lightening plant essential oil, wherein the raw materials of the plant essential oil include, by weight percentage: 30.0 g of frankincense oil; 20.0 g of sandalwood oil; 15.0 g of lavender oil; 12.0 g of myrrh oil; 12.0 g of Italian immortelle oil; 0.5 g of rock rose oil; 9.9 g of damask rose oil; 0.5 g of vetiver root oil; and 0.1 g of marine sterol. The marine sterol is a mixture of sterol from sterol from sterol from sterol from sterol from sterol from sterol from sterol from sterol from sterol from sterol from sterol from sterol, and the weight ratio of sterol from sterol from sterol from sterol from sterol from sterol is 1:3.

[0031] The preparation method of the natural firming, anti-wrinkle and skin-lightening plant essential oil comprises the following steps: S1. Weigh frankincense oil, sandalwood oil, lavender oil, myrrh oil, Italian immortelle oil, rock rose oil, taurine rose oil and vetiver root oil according to weight ratio and place them in a container, stir and mix at a rate of 150 r / min for 4 h under heating conditions of 35° C. to mix the essential oils until they are homogeneous, thereby obtaining a mixed essential oil; S2. Preparation of marine sterols: S2-1. Use conventional extraction and separation technology, specifically: take fresh stalked tunicate, wash and dry it, crush it into particles with a particle size of ≤0.2 mm, add the particles to a 90% ethanol solution for leaching, filter and take the filtrate after leaching, concentrate it under reduced pressure to obtain a primary extract, then extract the primary extract with ethyl acetate, and then concentrate it under reduced pressure to obtain a secondary extract, then add the secondary extract to a 90% methanol solution, extract it with n-hexane, and then concentrate it under reduced pressure to obtain a n-hexane extract, perform normal pressure silica gel column chromatography on the n-hexane extract, perform gradient elution with chloroform / methanol eluent, and the gradient volume ratio varies from 100:0 to 50:50, monitor by TLC, combine the same parts and concentrate them, and then perform Sephadex LH-20 gel column chromatography, the eluent is methanol:chloroform=1:1, and two components are obtained, and the colorless needle crystal component is taken, which is stalked tunicate sterol; S2-2, using conventional extraction and separation technology, specifically: taking fresh soft coral, washing and drying, crushing into particles with a particle size of ≤0.2mm, adding the particles to a 90% ethanol solution for leaching, filtering and taking the filtrate after leaching, decompressing and concentrating to an extract, distributing the extract with ethyl acetate / water, taking the ethyl acetate extract and decompressing and concentrating to obtain an ethyl acetate extract, and then subjecting the extract to column chromatography with silica gel H, gradient eluting with petroleum ether / ethyl acetate eluent of gradually increasing polarity, and successively obtaining two components from the eluent with a volume ratio of petroleum ether:ethyl acetate=20:80, taking the colorless columnar crystal component therein, i.e., soft coral sterol; S2-3, taking the sterol of styraciflua and the sterol of soft coral of chrysogenum and mixing them evenly according to the weight ratio, so as to obtain marine sterol; S3. Add the marine sterols to the mixed essential oils according to weight ratio, and stir and mix at a rate of 250 r / min at 40° C. for 1 h to obtain the product.

[0032] Embodiment 2 A natural firming, anti-wrinkle and skin-lightening plant essential oil, the raw materials of the plant essential oil include, by weight percentage: 35.0g of frankincense oil, 17.5g of sandalwood oil, 12.5g of lavender oil, 10.0g of myrrh oil, 15.0g of Italian immortelle oil, 1.0g of rock rose oil, 8.0g of damascena rose oil, 0.8g of vetiver root oil, and 0.2g of marine sterol. Among them, the marine sterol is a mixture of sterols of scutellariae and sterols of soft corals of scutellariae, and the weight ratio of sterols of scutellariae and sterols of soft corals of scutellariae is 1:3.

[0033] The preparation method of the natural firming, anti-wrinkle and skin-lightening plant essential oil comprises the following steps: S1. Weigh frankincense oil, sandalwood oil, lavender oil, myrrh oil, Italian immortelle oil, rock rose oil, tamarack rose oil and vetiver root oil according to weight ratio and place them in a container, and stir and mix them at a rate of 200 r / min under heating conditions of 38° C. for 3 h to mix the essential oils until they are homogeneous, thereby obtaining a mixed essential oil; S2. Preparation of marine sterols: S2-1. Use conventional extraction and separation technology, specifically: take fresh stalked tunicate, wash and dry it, crush it into particles with a particle size of ≤0.2 mm, add the particles to a 90% ethanol solution for leaching, filter and take the filtrate after leaching, concentrate it under reduced pressure to obtain a primary extract, then extract the primary extract with ethyl acetate, and then concentrate it under reduced pressure to obtain a secondary extract, then add the secondary extract to a 90% methanol solution, extract it with n-hexane, and then concentrate it under reduced pressure to obtain a n-hexane extract, perform normal pressure silica gel column chromatography on the n-hexane extract, perform gradient elution with chloroform / methanol eluent, and the gradient volume ratio varies from 100:0 to 50:50, monitor by TLC, combine the same parts and concentrate them, and then perform Sephadex LH-20 gel column chromatography, the eluent is methanol:chloroform=1:1, and two components are obtained, and the colorless needle crystal component is taken, which is stalked tunicate sterol; S2-2, using conventional extraction and separation technology, specifically: taking fresh soft coral, washing and drying, crushing into particles with a particle size of ≤0.2mm, adding the particles to a 90% ethanol solution for leaching, filtering and taking the filtrate after leaching, decompressing and concentrating to an extract, distributing the extract with ethyl acetate / water, taking the ethyl acetate extract and decompressing and concentrating to obtain an ethyl acetate extract, and then subjecting the extract to column chromatography with silica gel H, gradient eluting with petroleum ether / ethyl acetate eluent of gradually increasing polarity, and successively obtaining two components from the eluent with a volume ratio of petroleum ether:ethyl acetate=20:80, taking the colorless columnar crystal component therein, i.e., soft coral sterol; S2-3, taking the sterol of styraciflua and the sterol of soft coral of chrysogenum and mixing them evenly according to the weight ratio, so as to obtain marine sterol; S3. Add the marine sterols to the mixed essential oils according to weight ratio, and stir and mix at a rate of 300 r / min at 42° C. for 0.6 h to obtain the product.

[0034] Embodiment 3 A natural firming, anti-wrinkle and skin-lightening plant essential oil, wherein the raw materials of the plant essential oil include, by weight percentage: 40.0g of frankincense oil; 15.5g of sandalwood oil; 10.0g of lavender oil; 8.0g of myrrh oil; 18.0g of Italian immortelle oil; 1.5g of rock rose oil; 5.0g of damascena rose oil; 1.5g of vetiver root oil; and 0.5g of marine sterol. The marine sterol is a mixture of sterol from sterol from sterol from sterol from sterol from sterol from sterol from sterol from sterol from sterol from sterol from sterol, and the weight ratio of sterol from sterol from sterol from sterol from sterol from sterol is 1:3.

[0035] The preparation method of the natural firming, anti-wrinkle and skin-lightening plant essential oil comprises the following steps: S1. Weigh frankincense oil, sandalwood oil, lavender oil, myrrh oil, Italian immortelle oil, rock rose oil, tamarack rose oil and vetiver root oil according to weight ratio and place them in a container, stir and mix at a rate of 300 r / min under heating conditions of 40° C. for 2 h to mix the essential oils until they are homogeneous, thereby obtaining a mixed essential oil; S2. Preparation of marine sterols: S2-1. Use conventional extraction and separation technology, specifically: take fresh stalked tunicate, wash and dry it, crush it into particles with a particle size of ≤0.2 mm, add the particles to a 90% ethanol solution for leaching, filter and take the filtrate after leaching, concentrate it under reduced pressure to obtain a primary extract, then extract the primary extract with ethyl acetate, and then concentrate it under reduced pressure to obtain a secondary extract, then add the secondary extract to a 90% methanol solution, extract it with n-hexane, and then concentrate it under reduced pressure to obtain a n-hexane extract, perform normal pressure silica gel column chromatography on the n-hexane extract, perform gradient elution with chloroform / methanol eluent, and the gradient volume ratio varies from 100:0 to 50:50, monitor by TLC, combine the same parts and concentrate them, and then perform Sephadex LH-20 gel column chromatography, the eluent is methanol:chloroform=1:1, and two components are obtained, and the colorless needle crystal component is taken, which is stalked tunicate sterol; S2-2, using conventional extraction and separation technology, specifically: taking fresh soft coral, washing and drying, crushing into particles with a particle size of ≤0.2mm, adding the particles to a 90% ethanol solution for leaching, filtering and taking the filtrate after leaching, decompressing and concentrating to an extract, distributing the extract with ethyl acetate / water, taking the ethyl acetate extract and decompressing and concentrating to obtain an ethyl acetate extract, and then subjecting the extract to column chromatography with silica gel H, gradient eluting with petroleum ether / ethyl acetate eluent of gradually increasing polarity, and successively obtaining two components from the eluent with a volume ratio of petroleum ether:ethyl acetate=20:80, taking the colorless columnar crystal component therein, i.e., soft coral sterol; S2-3, taking the sterol of styraciflua and the sterol of soft coral of chrysogenum and mixing them evenly according to the weight ratio, so as to obtain marine sterol; S3. Add the marine sterols to the mixed essential oils according to weight ratio, and stir and mix at a rate of 350 r / min at 45° C. for 0.2 h to obtain the product.

[0036] Comparative Example 1 A natural firming, anti-wrinkle and skin-lightening plant essential oil. The raw materials of the plant essential oil include, by weight percentage: 35.0g of frankincense oil, 17.5g of sandalwood oil, 12.5g of lavender oil, 10.0g of myrrh oil, 15.0g of Italian immortelle oil, 1.0g of cistus oil, 8.0g of turkish rose oil and 1.0g of vetiver root oil.

[0037] The preparation method of the natural firming, anti-wrinkle and skin-lightening plant essential oil comprises the following steps: S1. Weigh frankincense oil, sandalwood oil, lavender oil, myrrh oil, Italian immortelle oil, rock rose oil, taurine rose oil and vetiver root oil according to weight ratio and place them in a container. Stir and mix them at a rate of 200 r / min for 3 h under heating conditions of 38° C. to mix the essential oils until they are homogeneous.

[0038] Comparative Example 2 A natural firming, anti-wrinkle and skin-lightening plant essential oil, wherein the raw materials of the plant essential oil include, by weight percentage: 35.0 g of frankincense oil, 17.5 g of sandalwood oil, 12.5 g of lavender oil, 10.0 g of myrrh oil, 15.0 g of Italian immortelle oil, 1.0 g of rock rose oil, 8.0 g of damascena rose oil, 0.8 g of vetiver root oil, and 0.2 g of marine sterol, wherein the marine sterol is sterol.

[0039] The preparation method of the natural firming, anti-wrinkle and skin-lightening plant essential oil comprises the following steps: S1. Weigh frankincense oil, sandalwood oil, lavender oil, myrrh oil, Italian immortelle oil, rock rose oil, tamarack rose oil and vetiver root oil according to weight ratio and place them in a container, and stir and mix them at a rate of 200 r / min under heating conditions of 38° C. for 3 h to mix the essential oils until they are homogeneous, thereby obtaining a mixed essential oil; S2. Preparation of marine sterols: The conventional extraction and separation technology is adopted, specifically: fresh stalked sea squirt is taken, washed and dried, and crushed into particles with a particle size of ≤0.2 mm, the particles are added to a 90% ethanol solution for leaching, the filtrate is filtered after leaching, and concentrated under reduced pressure to obtain a primary extract, then the primary extract is extracted with ethyl acetate, and then concentrated under reduced pressure to obtain a secondary extract, and then the secondary extract is added to a 90% methanol solution, extracted with n-hexane, and then concentrated under reduced pressure to obtain a n-hexane extract, the n-hexane extract is subjected to normal pressure silica gel column chromatography, and gradient elution is performed with chloroform / methanol eluent, and the gradient volume ratio varies from 100:0 to 50:50, TLC monitoring is performed, the same parts are combined and concentrated, and then Sephadex LH-20 gel column chromatography is performed, and the eluent is methanol:chloroform=1:1, and two components are obtained, and the colorless needle crystal component is taken, which is the stalked sea squirt sterol; S3. Add the marine sterols to the mixed essential oils according to weight ratio, and stir and mix at a rate of 300 r / min at 42° C. for 0.6 h to obtain the product.

[0040] Comparative Example 3 A natural firming, anti-wrinkle and skin-lightening plant essential oil, wherein the raw materials of the plant essential oil include, by weight percentage: 35.0g of frankincense oil, 17.5g of sandalwood oil, 12.5g of lavender oil, 10.0g of myrrh oil, 15.0g of Italian immortelle oil, 1.0g of rock rose oil, 8.0g of damascena rose oil, 0.8g of vetiver root oil, and 0.2g of marine sterols, wherein the marine sterols are sterols of soft coral of Sarcophagnum morifolium.

[0041] The preparation method of the natural firming, anti-wrinkle and skin-lightening plant essential oil comprises the following steps: S1. Weigh frankincense oil, sandalwood oil, lavender oil, myrrh oil, Italian immortelle oil, rock rose oil, tamarack rose oil and vetiver root oil according to weight ratio and place them in a container, and stir and mix them at a rate of 200 r / min under heating conditions of 38° C. for 3 h to mix the essential oils until they are homogeneous, thereby obtaining a mixed essential oil; S2. Preparation of marine sterols: A conventional extraction and separation technology is used, specifically: fresh soft coral of Sarcophyton fasciatus is taken, washed and dried, and crushed into particles with a particle size of ≤0.2 mm, the particles are added to an ethanol solution with a concentration of 90% for leaching, the filtrate is filtered after leaching, and the filtrate is concentrated under reduced pressure to obtain an extract, the extract is distributed with ethyl acetate / water, the ethyl acetate extract is concentrated under reduced pressure to obtain an ethyl acetate extract, and the extract is subjected to column chromatography with silica gel H, and gradient elution is performed with petroleum ether / ethyl acetate eluents of gradually increasing polarity, and two components are obtained in sequence from the eluent with a volume ratio of petroleum ether:ethyl acetate=20:80, and the colorless columnar crystal component is taken, which is the Sarcophyton fasciatus soft coral sterol; S3. Add the marine sterols to the mixed essential oils according to weight ratio, and stir and mix at a rate of 300 r / min at 42° C. for 0.6 h to obtain the product.

[0042] In addition, based on Example 3, the amount of marine sterols was gradually increased, and the process parameters were kept unchanged to obtain the following comparative examples 4-6 (Table 1): Table 1: Amounts of each component in Comparative Examples 4-6

[0043] 3. Safety and skin care effect testing The plant essential oils prepared in the above Examples 1-3 and Comparative Examples 1-6 were taken and diluted with sweet almond oil as a base oil (the dilution ratio was 1:100) to obtain essential oil samples to be tested. The essential oil samples were tested as follows: (1) Safety testing: With reference to the test method in Chapter 7 of the "Technical Specifications for Safety of Cosmetics (2015 Edition)", a human skin patch test was adopted, and 100 volunteers (actual age ranged from 20 to 55 years old) were selected as subjects (except for patients with insulin-dependent diabetes, patients with asthma or other chronic respiratory diseases who are receiving treatment, those with highly sensitive constitutions, women in lactation or pregnancy, etc., and the selected qualified volunteers signed informed consent forms), to detect the potential possibility of plant essential oils in the above embodiments and comparative examples causing adverse reactions to human skin. The specific detection process is: put the essential oil sample into the small chamber of the patch tester, the amount is 0.02ml, the control well is distilled water as a blank control, the patch tester with the sample is applied to the curved side of the forearm of the subject, and the palm is gently pressed to make it evenly applied to the skin, and it lasts for 24 hours. Observe the skin reaction according to the grading standard in Table 2 below 30 minutes, 24 hours, and 48 hours after removing the test object patch tester, and record the results.

[0044] Table 2: Occluded patch skin reaction grading criteria

[0045] (2) Anti-wrinkle effect test: Referring to the "Test Method for Anti-wrinkle Efficacy of Cosmetics (T / ZHCA006-2019)", 270 Chinese female volunteers with crow's feet level ≥ 2 points were selected (actual age ranged from 35-62 years old, qualified volunteers were selected and signed informed consent), and there were no interference factors that obviously affected the test results in the test area: birthmarks, acne, scratches, moles, etc., except for women in lactation, pregnancy or family planning period; people with allergic constitution, allergic dermatitis, and those who are receiving dermatological treatment, etc. The test environment temperature was (21.0±1.0)℃, and the test environment humidity was 50%±10%. The volunteers were randomly divided into 9 groups, with 30 volunteers in each group. Each group used the essential oil samples of the embodiments and comparative examples for 4 weeks. Photos were taken using the Primos-CR-3D skin analyzer at week 0, week 2, and week 4, respectively (note: the facial skin was cleaned with clean water before taking photos. After cleaning, the volunteers sat in a constant temperature and humidity room for 30 minutes before taking photos). The parameters of crow's feet, including number, depth, area, and length, were analyzed, and the results were recorded (the average value of each group was taken).

[0046] (3) Skin lightening effect test: 270 volunteers were selected, and the inclusion criteria were: healthy Chinese women, 18-45 years old; insensitive to commonly used skin care products; high compliance. Exclusion criteria: those who have used antihistamines in the past week or immunosuppressants in the past month; those who have used any anti-inflammatory drugs in the test area in the past two months; insulin-dependent diabetics; patients with respiratory diseases who are receiving treatment; people with allergies, allergic dermatitis, etc., with a history of skin diseases; people who are receiving dermatological treatment or taking hydroxy acids, whitening and anti-aging drugs within one month; those with large birthmarks, scratches, white spots, pigmented moles, keloids and other skin manifestations that affect the test in the test area. Qualified volunteers signed informed consent.

[0047] The volunteers were randomly divided into 9 groups, 30 in each group, and placed in a constant temperature and humidity test environment with a temperature of (21.0±1.0)℃ and a humidity of 50%±10%. Before the test, the subjects first cleaned their faces as required, rested for 20 minutes in a constant temperature and humidity room, and used Visia-CR standard light to collect facial images as the initial facial images before applying the samples; then, the essential oil samples of each embodiment and comparative example were used for each group of volunteers, at a concentration of 2mg / cm 2 The dosage of the plant essential oil was applied to the skin until it was fully adhered to the skin, and the second facial image was collected using Visia-CR; 8 hours after application, the facial image was collected again using Visia-CR to obtain the third facial image.

[0048] Perform skin color analysis on the above standard light image, select the cheek or forehead area as the AOI area, apply the Lab color channel of the calculation macro part, obtain the Lab mean, extract the L value representing the skin color brightness, calculate the ITA° value (individual type angle) representing the whiteness of the skin color, and record the results.

[0049] IV. Results Analysis (1) Safety testing: All 100 volunteers completed the 24-hour skin occlusive patch test of each embodiment and comparative example. After removing the patch tester for 30 minutes, 24 hours, and 48 hours, the essential oil samples of each embodiment and comparative example did not produce adverse reactions on the skin, that is, all were grade 0 negative reactions.

[0050] (2) Anti-wrinkle effect test: After the subjects used the plant essential oils continuously, the skin wrinkle analysis system Primos was used to evaluate the anti-wrinkle effect of the essential oils. The evaluation results were statistically obtained. Figure 1-4 It can be seen that the number of wrinkles, wrinkle depth, wrinkle area and wrinkle length of the essential oil samples prepared in Examples 1-3 of the present invention were significantly improved after 4 weeks of use, especially Example 2, which was superior to Examples 1 and 3 in all aspects.

[0051] Comparative Example 1 did not add marine sterols to the formula, so the anti-wrinkle effect was significantly worse than that of Example 2; Comparative Examples 2 and 3 only added one ingredient, sterols from sclerotinia or sterols from soft corals, respectively, and the anti-wrinkle effect was also significantly worse than that of Example 2, and was only slightly improved compared to Comparative Example 1, which shows that sterols from sclerotinia and sterols from soft corals must be added at the same time in proportion to achieve the best effect, otherwise the effect is not obvious; Comparative Examples 4-6 gradually increased the use of marine sterols on the basis of Example 3. It can be seen that when the amount of marine sterols is 0.7g, the effect is basically equivalent to that of Example 3, and each has its own advantages and disadvantages, indicating that the additional 0.2g added has no effect, and when the amount of marine sterols is 0.9g and 1.1g, the anti-wrinkle effect becomes worse and worse, and is inferior to Example 3 in all aspects. When the amount reaches 1.1g, the anti-wrinkle effect is even inferior to that of not adding marine sterols in some aspects. This shows that the more marine sterols are used, the better, and only when it is less than 0.5% can it exert a better effect.

[0052] (3) Skin lightening effect test: Table 3: Skin lightening effect test results

[0053] As can be seen from Table 3, after using the essential oil samples prepared in Examples 1-3 of the present invention, the whiteness and brightness of the skin can be significantly improved immediately, and after 8 hours of use, the level can be maintained basically equivalent to that immediately after use, indicating that the plant essential oil of the present invention can significantly brighten the skin color.

[0054] However, in Comparative Example 1, no marine sterols were added to the formula, so that the skin lightening effect was significantly worse than that of Example 2; in Comparative Example 2 and Comparative Example 3, only one ingredient, scutellaria sterol or scutellaria sterol, was added respectively, and the skin lightening effect was also significantly worse than that of Example 2, and was only slightly improved compared with Comparative Example 1, which shows that scutellaria sterol and scutellaria sterol must be added simultaneously in proportion to achieve the best effect, otherwise the effect is not obvious; Comparative Examples 4-6 gradually increase the amount of marine sterols on the basis of Example 3, and it can be seen that there is a difference from the anti-wrinkle effect here. When the amount of marine sterols exceeds 0.5g, the skin lightening effect becomes worse and worse with the increase in the amount, which is inferior to Example 3 in all aspects, further reflecting that the amount of marine sterols is not the more the better, and only in the range of less than 0.5% can a better effect be achieved.

[0055] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A natural firming, anti-wrinkle and skin-lightening plant essential oil, characterized in that: By weight percentage, the raw materials of the plant essential oil include: Frankincense oil 30.0-40.0%; Sandalwood oil 15.0-20.0%; Lavender oil 10.0-15.0%; Myrrh oil 8.0-12.0%; Italian Immortelle flower oil 12.0-18.0%; Cistus oil 0.5-1.5%; Rosa damascena flower oil 5.0-10.0%; Vetiver root oil 0.5-1.5%; Marine sterols 0.1-0.5%.

2. The natural firming, anti-wrinkle and skin-lightening plant essential oil according to claim 1, characterized in that: By weight percentage, the raw materials of the plant essential oil include: Frankincense oil 35.0%; Sandalwood oil 17.5%; Lavender oil 12.5%; Myrrh oil 10.0%; Italian Immortelle flower oil 15.0%; Cistus oil 1.0%; Rosa damascena flower oil 8.0%; Vetiver root oil 0.8%; Marine sterols 0.2%.

3. The natural firming, anti-wrinkle and skin-lightening plant essential oil according to claim 1, characterized in that: The marine sterol is a mixture of ascidian sterol and sterol from the soft coral of Sarcophyton.

4. The natural firming, anti-wrinkle and skin-lightening plant essential oil according to claim 3, characterized in that: The weight ratio of the sterol to the sterol is 1:

3.

5. A method for preparing the natural firming, anti-wrinkle and skin-lightening plant essential oil according to any one of claims 1 to 4, characterized in that the steps include: S1. Weigh frankincense oil, sandalwood oil, lavender oil, myrrh oil, Italian immortelle oil, rock rose oil, turkish rose oil and vetiver root oil according to weight ratio and place them in a container and mix until homogeneous to obtain a mixed essential oil; S2, preparation of marine sterols; S3. Add the marine sterols to the mixed essential oils according to the weight ratio, and mix evenly again to obtain the product.

6. The method for preparing the natural firming, anti-wrinkle and skin-lightening plant essential oil according to claim 5, characterized in that: In step S1, the specific operation of mixing until homogeneous is: stirring and mixing at a rate of 150-300 r / min for 2-4 hours under heating conditions of 35-40°C.

7. The method for preparing the natural firming, anti-wrinkle and skin-lightening plant essential oil according to claim 5, characterized in that: The specific operations of step S2 are: S2-1, take fresh stalked sea squirt, wash and dry it, crush it into particles with a particle size of ≤0.2mm, add the particles to a 90% ethanol solution for leaching, filter and take the filtrate after leaching, concentrate it under reduced pressure to obtain a primary extract, then extract the primary extract with ethyl acetate, and then concentrate it under reduced pressure to obtain a secondary extract, then add the secondary extract to a 90% methanol solution, extract it with n-hexane, and then concentrate it under reduced pressure to obtain a n-hexane extract, perform normal pressure silica gel column chromatography on the n-hexane extract, perform gradient elution with chloroform / methanol eluent, and the gradient volume ratio varies from 100:0 to 50:50, monitor by TLC, combine the same parts and concentrate them, and then perform Sephadex LH-20 gel column chromatography, the eluent is methanol:chloroform=1:1, and two components are obtained, and the colorless needle crystal component is taken, which is stalked sea squirt sterol; S2-2, take fresh soft coral, wash and dry it, crush it into particles with a particle size of ≤0.2mm, add the particles to an ethanol solution with a concentration of 90%, extract it, filter and take the filtrate after extraction, decompress and concentrate it to an extract, distribute the extract with ethyl acetate / water, take the ethyl acetate extract and decompress and concentrate it to obtain an ethyl acetate extract, and then perform column chromatography on the extract with silica gel H, perform gradient elution with petroleum ether / ethyl acetate eluent of gradually increasing polarity, and obtain two components in sequence from the eluent with a volume ratio of petroleum ether: ethyl acetate = 20:80, and take the colorless columnar crystal component therein, which is the soft coral sterol; S2-3, taking the sterol of sclerotinia and the sterol of soft coral of phytohormone and mixing them evenly according to the weight ratio, so as to obtain marine sterol.

8. The method for preparing the natural firming, anti-wrinkle and skin-lightening plant essential oil according to claim 5, characterized in that: In step S3, the specific operation of mixing uniformly is: stirring and mixing at a rate of 250-350 r / min for 0.2-1 h under heating conditions of 40-45°C.

9. Use of the natural firming, anti-wrinkle and skin-lightening plant essential oil as claimed in any one of claims 1 to 4 in skin care products.

10. The use according to claim 9, characterized in that: The skin care product is one of an aqueous solution, a facial mask, an essence, an emulsion, a facial cream, and a liquid foundation.

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