Composition with effects of nourishing hair and reducing alopecia as well as preparation method and application of composition

Abstract

The invention belongs to the technical field of biology, and relates to a novel application of prinsepia utilis royle extract in the hair care field, in particular to a composition with the effects of nourishing hair and reducing alopecia as well as a preparation method and application of the composition. The preparation method of the prinsepia utilis royle extract comprises the following steps: T1, taking prinsepia utilis royle, washing, drying, crushing, and extracting by adopting a supercritical CO2 extraction method to obtain prinsepia utilis royle oil and prinsepia utilis royle meal; t2, taking prinsepia utilis royle meal, and performing crushing and enzymolysis to obtain enzymatic hydrolysate; t3, inoculating yarrowia lipolytica into the fermentation culture medium added with the enzymatic hydrolysate for fermentation, centrifuging, taking supernatant, and filtering to obtain fermentation supernatant; and T4, mixing the fermented supernatant with prinsepia utilis royle oil, and homogenizing to obtain the prinsepia utilis royle extract. The prinsepia utilis royle extract disclosed by the invention is high in stability, has the effect of increasing the moisture retention of hair, can reduce alopecia by promoting human hair papilla cells to secrete VEGF, and is safe and non-irritant to human skin.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and relates to a new use of a prickle fruit extract in the field of hair care, and more specifically, to a composition with the effects of nourishing hair and reducing hair loss, and a preparation method and application thereof. Background Art

[0002] Prinsepia utilis Royle is a plant of the genus Prinsepia in the Rosaceae family, and Prinsepia utilis Royle oil is the main active ingredient in Prinsepia utilis, which contains most of the unsaturated fatty acids and vitamins. In addition, Prinsepia utilis Royle is also rich in antioxidant peptides, antibacterial peptides, polysaccharides, flavonoids and polyphenol compounds. Studies have shown that Prinsepia utilis Royle oil and other related extracts have the effects of skin moisturizing, anti-oxidation, whitening and anti-inflammatory.

[0003] At present, the uses of prickle fruit extracts in hair care mostly involve hair moisturizing and repairing. For example, Chinese patent application CN114699340A discloses that a composition of prickle fruit oil, jojoba oil and squalane in a specific ratio can effectively repair damaged hair scales, maintain hair, and make hair smoother and more shiny; Chinese patent application CN115919692A discloses that a composition including prickle fruit oil, olive oil and papaya oil has a dual moisturizing effect on hair and scalp. However, none of the above prior arts involve the use of prickle fruit extracts in increasing the moisture content of hair and reducing hair loss. Summary of the invention

[0004] The present invention aims to provide a new use of the prickle fruit extract in increasing the moisture content of hair and reducing hair loss, and the properties of the prickle fruit extract are more stable, and this new property is only manifested in the prickle fruit extract obtained by a specific preparation process. The above-mentioned specific preparation process includes the use of specific process steps, such as enzymatic hydrolysis, the use of specific fermentation substrates, specific fermentation strains, etc. The combination of these specific factors enables the prepared prickle fruit extract to obtain new properties, which have not been discovered before the application date and are not easy to be discovered.

[0005] One of the inventive objectives of the present invention is to provide a use of a prickle fruit extract in preparing a hair care product having the effects of increasing the moisture content in hair and reducing hair loss.

[0006] Furthermore, the preparation steps of the prickle fruit extract are as follows:

[0007] T1, taking Prinia prinia, washing, drying, crushing, and extracting by supercritical CO2 extraction to obtain Prinia prinia oil and Prinia prinia meal;

[0008] T2, taking the fruit meal of the thorn tree, crushing it, and performing enzymolysis to obtain an enzymolysis solution;

[0009] T3, inoculating Yarrowia lipolytica into the fermentation medium added with the above enzymatic hydrolysate for fermentation, centrifuging, taking the supernatant, filtering, and obtaining the fermentation supernatant;

[0010] T4. Mix the fermentation supernatant with the prickle oil and homogenize to obtain the prickle extract.

[0011] In the comparative test, it was found that although the prickle oil has been disclosed to have the effect of moisturizing hair and scalp, in the test of improving hair moisturizing ability, the prickle oil only showed the effect of increasing the moisture content on the surface of the hair, but did not show the effect of increasing the moisture content inside the hair, and did not show the effect of reducing hair loss. Therefore, it can be seen from the analysis that the new properties of the prickle extract of the present invention do not depend on the presence of prickle oil.

[0012] In realizing the unique functional properties of the prickle fruit extract of the present invention, the step of enzymolysis of the prickle fruit meal with a specific enzyme is indispensable, because when other parameters remain unchanged, the supernatant obtained by fermenting the prickle fruit meal without enzymolysis does not show similar functions after being mixed with the prickle fruit oil; and the type of enzyme used for enzymolysis is also very important, and different enzymes will also lead to some differences in the properties of the prickle fruit extract.

[0013] In addition, the composition of the extract is also critical. The prickle fruit extract of the present invention is essentially composed of prickle fruit oil and the fermentation supernatant of prickle fruit meal enzymatic hydrolyzate. However, when the two are used alone, they do not show the functional properties of combined use, which indicates that this functional property is produced when the two work together.

[0014] Secondly, the type of fermented bacteria also affects the functional properties of the product. Yeast fermentation only shows a moisturizing effect but cannot reduce hair loss.

[0015] Furthermore, the supercritical CO2 extraction process parameters are: extraction pressure of 10-20 MPa, CO2 flow rate of 20-50 L / min, extraction temperature of 35-40°C, and extraction time of 1-3 h.

[0016] Furthermore, in step T2, the enzyme used for enzymolysis is alkaline protease.

[0017] Furthermore, in step T2, the enzymatic hydrolysis pH is 7.0-8.0, the enzyme mass fraction is 1.0-3.5%, the enzymatic hydrolysis time is 3-6 hours, and the solid-liquid ratio is 1:20-35 g / mL.

[0018] Furthermore, in step T3, the amount of enzymatic solution added to the fermentation medium is 5-20%.

[0019] Furthermore, in step T3, the inoculation amount of the Yarrowia lipolytica is 1-3%; the fermentation conditions are: temperature 25-30° C., fermentation time 24-72 h, and shaking speed 150-300 r / min.

[0020] Furthermore, in step T3, the centrifugation conditions are: 3000-5000 r / min, 5-10 min.

[0021] Furthermore, in step T4, the fermentation supernatant and the prickle oil are mixed in a ratio of 10:1 to 5.

[0022] Another object of the present invention is to provide the prickle fruit extract.

[0023] Another object of the present invention is to provide a shampoo having the effects of nourishing hair and reducing hair loss, comprising the prune fruit extract.

[0024] The present invention has the following beneficial effects:

[0025] The prickle fruit extract of the present invention is composed of prickle fruit oil and supernatant obtained by enzymatic fermentation of prickle fruit meal in a specific ratio. Although the extract contains prickle fruit oil, it is surprisingly stable and has a remarkable effect on increasing the moisture content in hair. In addition, it has a new property that it can reduce hair loss by promoting the secretion of VEGF by human hair papilla cells and is safe and non-irritating to human skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 The hair growth of mice in each group was scored. DETAILED DESCRIPTION

[0027] The present invention is further described below with reference to specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0028] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0029] Activation of Yarrowia lipolytica strains and cultivation of seed solution:

[0030] Yarrowia lipolytica (purchased from Wuhan Huaerna Biotechnology Co., Ltd., catalog number: WN-BZ13748) was streaked onto YPD solid culture medium and cultured at a constant temperature of 25°C for 2-3 days. A single colony on the YPD solid culture medium was picked up and inoculated into YPD liquid culture medium, which was placed in a shaker and cultured at a constant temperature of 25°C and 200 r / min for 24 hours to obtain a seed solution.

[0031] Among them, yeast peptone glucose (YPD) solid culture medium: glucose 20.0 g / L, yeast powder 10.0 g / L, peptone 20.0 g / L, agar 20.0 g / L.

[0032] YPD liquid culture medium: glucose 20.0 g / L, yeast powder 10.0 g / L, peptone 20.0 g / L.

[0033] Fermentation medium: yeast powder 0.75 g / L, KH2PO4 1.00 g / L, MgSO4·7H2O 1.00 g / L, (NH4)2SO4 2.50 g / L and NaCl 20.0 g / L.

[0034] Example 1: Preparation of Prinus utilis extract

[0035] T1. Take the prickle fruit, wash it, dry it in a constant temperature drying oven at 45°C for 3h, and crush it; add the crushed prickle fruit into a supercritical carbon dioxide reactor, extract it for 2h under the conditions of extraction pressure of 15MPa, CO2 flow rate of 25L / min, and extraction temperature of 40°C, collect volatile oil, and obtain prickle fruit oil and prickle fruit meal;

[0036] T2, taking the pruning thorn fruit meal, crushing it, adding distilled water to the crushed pruning thorn fruit meal at a solid-liquid ratio of 1:30 g / mL, and then adding 1.5 wt % alkaline protease, enzymolyzing it at a pH of 8.0 for 5 h, and filtering it with a 0.22 μm microporous filter membrane to obtain an enzymolysis solution;

[0037] T3, adding 10wt% of the enzymatic hydrolysate to the fermentation medium, and then inoculating 1wt% of the Yarrowia lipolytica seed solution, fermenting for 48h at a temperature of 28°C and a shaking speed of 200r / min, collecting the fermentation broth, centrifuging at 3000r / min for 5min, taking the supernatant and filtering it through a 0.22μm microporous membrane, and sterilizing at 95°C for 30min to obtain the fermentation supernatant;

[0038] T4. Mix the fermentation supernatant and the prickle oil in a weight ratio of 10:3, and homogenize to obtain the prickle extract.

[0039] Example 2: Preparation of Prinus utilis extract

[0040] T1, take the prickle fruit, wash it, dry it in a constant temperature drying oven at 45°C for 2h, crush it, add the crushed prickle fruit into a supercritical carbon dioxide reactor, extract it for 1.5h under the conditions of extraction pressure of 10MPa, CO2 flow rate of 20L / min, and extraction temperature of 35°C, collect volatile oil, and obtain prickle fruit oil and prickle fruit meal;

[0041] T2, taking the pruning thorn fruit meal, crushing it, adding distilled water to the crushed pruning thorn fruit meal at a solid-liquid ratio of 1:25 g / mL, and then adding 1.0 wt % alkaline protease, enzymolyzing it for 4 h at a pH of 8.0, and filtering it with a 0.22 μm microporous filter membrane to obtain an enzymolysis solution;

[0042] T3, adding 5wt% of the enzymatic hydrolysate to the fermentation medium, and then inoculating 1.5wt% of the Yarrowia lipolytica seed solution, fermenting for 72h at a temperature of 26°C and a shaking speed of 150r / min, collecting the fermentation broth, centrifuging at 3000r / min for 5min, taking the supernatant and filtering it through a 0.22μm microporous membrane, and sterilizing at 95°C for 30min to obtain the fermentation supernatant;

[0043] T4. Mix the fermentation supernatant and the prickle oil in a weight ratio of 10:5, and homogenize to obtain the prickle extract.

[0044] Example 3: Preparation of Prinus utilis extract

[0045] T1, take the prickle fruit, wash it, dry it in a constant temperature drying oven at 45°C for 2.5h, crush it, add the crushed prickle fruit into a supercritical carbon dioxide reactor, extract it for 1h under the conditions of extraction pressure of 20MPa, CO2 flow rate of 50L / min, and extraction temperature of 35°C, collect volatile oil, and obtain prickle fruit oil and prickle fruit meal;

[0046] T2, taking the pruning thorn fruit meal, crushing it, adding distilled water to the crushed pruning thorn fruit meal at a solid-liquid ratio of 1:20 g / mL, and then adding 2.0 wt % alkaline protease, enzymolyzing it for 3 h at a pH of 8.0, and filtering it with a 0.22 μm microporous filter membrane to obtain an enzymolysis solution;

[0047] T3, adding 15wt% of the enzymatic hydrolysate to the fermentation medium, and then inoculating 2.0wt% of the Yarrowia lipolytica seed solution, fermenting for 36 hours at a temperature of 25°C and a shaking speed of 100r / min, collecting the fermentation broth, centrifuging at 3000r / min for 5min, taking the supernatant and filtering it through a 0.22μm microporous membrane, and sterilizing at 95°C for 30min to obtain the fermentation supernatant;

[0048] T4. Mix the fermentation supernatant and the prickle oil in a weight ratio of 10:2, and homogenize to obtain the prickle extract.

[0049] Comparative Example 1

[0050] The difference from Example 1 is that the Prinia pritta extract is the fermentation supernatant prepared in steps T2 to T3, and does not include the Prinia pritta oil prepared in step T1.

[0051] Comparative Example 2

[0052] The difference from Example 1 is that the Prinia pritta extract is the Prinia pritta oil prepared in step T1.

[0053] Contrast three

[0054] The difference from Example 1 is that the T2 enzymatic hydrolysis step is omitted, and the thorn fruit meal obtained in the T1 step is directly used to ferment instead of the enzymatic hydrolysis liquid.

[0055] Comparative Example 4

[0056] The difference from Example 1 is that papain is used to replace alkaline protease in step T2, the pH of papain hydrolysis is 7.0, and the other parameters are the same.

[0057] Comparative Example 5

[0058] The difference from Example 1 is that in step T3, Saccharomyces cerevisiae is used instead of Yarrowia lipolytica.

[0059] Comparative Example 6

[0060] The hair moisturizing composition containing prickle fruit oil as described in Example 1 of Chinese patent application CN 115919692 A.

[0061] Test Example 1: Test to increase moisture content inside and outside hair

[0062] 1.1 Test subjects: The same hair bundles were selected and divided into a normal hair group (blank group) and a damaged dry hair group. The damaged dry hair was obtained by treating normal hair with a bleaching agent for 30 cm, washing it with warm water, and drying it at 50°C for 10 minutes.

[0063] 1.2 Treatment method: The damaged dry hair was randomly divided into a model group, Examples 1 to 3, and Comparative Examples 1 to 6. The extracts of Examples 1 to 3 and Comparative Examples 1 to 6 were dispersed in water to prepare a treatment agent with a concentration of 1000 ppm; each treatment agent was applied to the damaged dry hair, and the hair was treated in air isolation for 60 minutes. Each treatment agent was repeated 3 times, and the model group was not treated. The average value was calculated. The moisture content of the hair was measured using a moisture meter (MS-70, Japan A&D), the measurement temperature was 60°C and 180°C, and the hair was heated to 0.02% (w / w) of moisture content, and the cumulative water evaporation (%, w / w) within 30 minutes was recorded.

[0064] Table 1 Thermogravimetric analysis results

[0065] Treatment reagents Hair surface moisture content (60℃, %) Water content in hair (180℃, %) Blank Group 10.35±1.26 15.21±2.12 Model Group <![CDATA[5.29±0.45 ** ]]> <![CDATA[9.26±1.71 ** ]]> Embodiment 1 <![CDATA[18.42±1.37 ## ]]> <![CDATA[15.51±1.60 ## ]]> Embodiment 2 <![CDATA[16.18±1.86 ## ]]> <![CDATA[14.74±0.92 ## ]]> Embodiment 3 <![CDATA[17.67±1.05 ## ]]> <![CDATA[14.33±1.12 ## ]]> Comparative Example 1 <![CDATA[5.87±0.48 ^^ ]]> <![CDATA[9.32±0.54 ^^ ]]> Comparative Example 2 <![CDATA[10.52±0.35 ^^ ]]> <![CDATA[10.28±0.78 ^^ ]]> Comparative Example 3 <![CDATA[14.49±1.41 ^ ]]> <![CDATA[12.22±1.83 ^ ]]> Comparative Example 4 <![CDATA[13.25±0.74 ^^ ]]> <![CDATA[11.07±0.51 ^^ ]]> Comparative Example 5 <![CDATA[16.73±0.80 ^ ]]> <![CDATA[12.53±0.39 ^ ]]> Comparative Example 6 18.04±1.94 <![CDATA[13.93±1.26 ^ ]]>

[0066] Note: Compared with the blank group, ** P<0.01; compared with the model group, # P<0.05, ##P<0.01; compared with the embodiment group, ^ P<0.05, ^^ P<0.01.

[0067] As can be seen from the above table, the prickle oil has a significant effect of increasing the moisture content on the surface of the hair. Although the moisture content in the hair is increased, it is not significant enough (see Comparative Example 2). The fermentation supernatant of Yarrowia lipolytica from the prickle meal does not show a moisturizing effect on the hair (see Comparative Example 1). However, the prickle extract (Examples 1 to 3) composed of prickle oil and prickle meal fermentation supernatant in a specific ratio of the present invention shows a more significant effect of increasing the surface and internal moisture content of the hair than the prickle oil. From the results of other comparative examples, it can be seen that this effect is closely related to the type of enzyme used in the preparation process of the enzymatic hydrolyzate and the strain used in the fermentation process.

[0068] Experimental Example 2: Study on the efficacy of anti-hair loss

[0069] 1.1 Animal depilation: 100 Kunming mice, weighing 20±2g, were randomly divided into 10 groups, each with 10 mice, named blank group, model group, positive group (2% minoxidil), Example 1 and Comparative Example 1 to 6. After each group of mice was adaptively raised for 3 days before the experiment, rosin and paraffin were heated and mixed in a ratio of 1:1 and melted, and then applied to the back of each group of mice, with an area of ​​about 4cm×4cm, and removed after cooling and solidification.

[0070] 1.2 Modeling and drug administration: On the second day of hair removal, the mice were anesthetized and the hair removal area on the back of the mice in the blank group was heated at 0.1 mL / cm 2 50% ethanol solution was applied, and 1 mg / mL testosterone solution was applied to the other groups once a day for 28 consecutive days. 30 minutes after modeling every day, distilled water was applied to the depilated area of ​​the mice in the blank group and the model group, and the other groups were coated with corresponding drug solutions (the extracts of Examples 1 to 3 and Comparative Examples 1 to 6 were dispersed in water to prepare a treatment reagent with a concentration of 1000 ppm), and the administration volume was 0.2 mL per mouse.

[0071] 1.3 New hair growth score: The new hair growth of each group of mice was scored according to Table 2 on days 7, 14, 21 and 28 after hair removal. The results are shown in Table 2. Figure 1 shown.

[0072] Table 2 Scoring criteria

[0073] Scoring Criteria integral No hair growth 0 Light hair growing all over the hair removal area 1 The length and density of new hair is about half of the unremoved area. 2 The length of new hair is no different from that of the unremoved area 3

[0074] 1.4 Hair length and weight: On the second day after the last application of the drug (i.e., the 29th day after hair removal), the mice were killed by cervical dislocation, and 10 hairs were randomly plucked from each mouse, and the average value was taken as the length of the new hair of each mouse, and the average hair length of each group was calculated; a circular skin piece was taken from the same area of ​​the mouse with a 20mm punch, and then all the hair on the skin piece was scraped off with a scalpel, and weighed to calculate the average hair weight of each group of mice. The results are shown in Table 3 below.

[0075] 1.5 Mouse skin tissue and hair follicle number: At the end of the experiment, all mice were processed, and the skin tissue removed from the back was stained with HE. The longitudinal and cross-sections of the hair follicles were observed under a microscope (×400); the number of hair follicles, terminal hair and vellus hair were counted, and the ratio of terminal hair to vellus hair was calculated. The results are shown in Table 4.

[0076] 2 Results

[0077] Table 3 Hair length and weight of mice

[0078] Group Hair length / mm Gross weight / mg Blank control group 8.2145±0.4741 20.24±1.45 Model Group <![CDATA[6.8137±0.0483 ** ]]> <![CDATA[15.37±0.54 ** ]]> Positive group <![CDATA[8.0795±0.3135 ## ]]> <![CDATA[20.37±0.69 ## ]]> Embodiment 1 <![CDATA[7.9553±0.1166 ## ]]> <![CDATA[19.63±1.06 ## ]]> Comparative Example 1 <![CDATA[7.5142±0.2855 ^ ]]> <![CDATA[17.79±0.33 ^^ <!-- 5 -->]]> Comparative Example 2 <![CDATA[6.9057±0.1215 ^^ ]]> <![CDATA[16.07±0.82 ^^ ]]> Comparative Example 3 <![CDATA[7.1962±0.1543 ^^ ]]> <![CDATA[17.02±0.55 ^^ ]]> Comparative Example 4 <![CDATA[7.2539±0.2165 ^^ ]]> <![CDATA[17.33±0.49 ^^ ]]> Comparative Example 5 <![CDATA[6.8964±0.0437 ^^ ]]> <![CDATA[15.89±0.97 ^^ ]]> Comparative Example 6 <![CDATA[6.8215±0.0149 ^^ ]]> <![CDATA[15.44±0.42 ^^ ]]>

[0079] Note: Compared with the blank control group, * P<0.05, ** P<0.01, compared with the model group, # P<0.05, ## P<0.01, compared with the positive group, ^ P<0.05, ^^ P<0.01.

[0080] Table 4 Hair follicle number and terminal hair / vellus hair in mice

[0081]

[0082]

[0083] Note: Compared with the blank control group, * P<0.05, ** P<0.01, compared with the model group, # P<0.05, ## P<0.01, compared with the positive group, ^ P<0.05, ^^ P<0.01.

[0084] Results Analysis

[0085] analyze Figure 1As shown in Tables 3 and 4, compared with the model group, the hair length, hair weight, number of hair follicles and terminal hair / vellus hair ratio of the positive group and Example 1 group of mice were increased, and there were extremely significant differences (P < 0.01); compared with the positive group, the indicators of the comparative examples 1 to 6 groups were significantly or extremely significantly reduced, but the effect of Example 1 group was equivalent to that of the positive group, which shows that the extract of the fruit of the green thorn tree in Example 1 of the present invention has a significant effect of activating hair follicles and promoting hair growth, and the effect is equivalent to 2% minoxidil.

[0086] Test Example 3: Test on Promoting VEGF Secretion in Human Dermal Papilla Cells

[0087] The qualified human dermal papilla cells of the third generation were taken and cultured at 1×10 5 The wells were inoculated in a 96-well plate and cultured at 37°C and 5% CO2 for 24 hours. The culture medium was then discarded and 100 μL of serum-free MSCM culture medium containing the treatment reagents of each group was added to each well (the concentration of the treatment reagents in the culture medium was 1000 ppm). No treatment reagent was added to the blank control group. The cells were cultured for 3 days, and the supernatants were collected. The VEGF concentration in the supernatants of each group was detected using an ELISA kit. The results are shown in Table 5.

[0088] Table 5 Test results of promoting VEGF secretion by human dermal papilla cells

[0089]

[0090]

[0091] Note: Compared with the blank control group, * P<0.05, ** P<0.01, *** P<0.01; compared with the embodiment group, ## P<0.01, ### P<0.001.

[0092] As shown in Table 5, VEGF is a vascular endothelial growth factor, which can stimulate and promote the delivery of nutrients to the hair papilla and promote the nutrient supply to the hair follicles; the study found that the expression of VEGF in healthy people and people with hair loss was significantly different, and the expression of VEGF in healthy people was enhanced. The study found that the prickle fruit extract of the present invention can significantly promote the secretion of VEGF by human hair papilla cells, indicating that it has a potential anti-hair loss effect. And when a certain parameter in the preparation method is changed, the obtained prickle fruit extract does not show or shows a weaker similar effect.

[0093] Test Example 4: Safety Test

[0094] Human skin closed patch test: Place the treatment reagents of Examples 1 to 3 and Comparative Examples 1 to 6 in the small chamber of the patch tester, respectively, with a dosage of 0.020 to 0.025 ml, and apply the patch tester to the curved side of the forearm of the test subject, with one side as the blank control area and the other side as the test area, for 24 hours. Observe and record the results 30 minutes (until the indentation disappears), 24 hours and 48 hours after removing the test patch. Evaluate the results according to the grading standards of the "Technical Specifications for Safety of Cosmetics" (2015 edition).

[0095] The patch test results showed that all groups of subjects had negative reactions, indicating that the treatment reagents had no irritation to the skin.

[0096] Test Example 5: Stability

[0097] 300 mL of the prickle fruit extract prepared in Example 1 and Comparative Examples 2 to 5 were respectively stored at room temperature (25° C.) and 45° C. for 60 days, and the acid value and peroxide value were tested. The acid value and peroxide value at 0 day were compared, and the change rate was calculated. The results are shown in Table 6.

[0098] Acid value / peroxide value change rate = (after storage - before storage) / before storage × 100%

[0099] Table 6 Stability test results (change rate, %)

[0100]

[0101]

[0102] Note: The unit of acid value is mgKOH / g, and the unit of peroxide value is mmol / Kg.

[0103] As can be seen from the above table, the stability of the prickle fruit oil is poor. After being stored at room temperature (25°C) and 45°C for 60 days, the acid value and peroxide value change significantly (Comparative Example 2). Although the prickle fruit extract of Example 1 of the present invention contains prickle fruit oil, after the same treatment, the acid value and peroxide value change less, indicating that the prickle fruit extract of the present invention has better stability. By analyzing the results of other comparative examples, it can be seen that the improvement of this stability is largely due to the presence of the fermentation supernatant. When a certain parameter of the fermentation supernatant preparation is changed, it will lead to a large difference in stability.

[0104] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. Use of the prickle fruit extract in preparing a hair care product having the effects of increasing the moisture content in hair and reducing hair loss.

2. The use according to claim 1, characterized in that The preparation steps of the prickle fruit extract are as follows: T1, taking Prinia prinia, washing, drying, crushing, and extracting by supercritical CO2 extraction to obtain Prinia prinia oil and Prinia prinia meal; T2, taking the fruit meal of the thorn tree, crushing it, and performing enzymolysis to obtain an enzymolysis solution; T3, inoculating Yarrowia lipolytica into the fermentation medium added with the above enzymatic hydrolysate for fermentation, centrifuging, taking the supernatant, filtering, and obtaining the fermentation supernatant; T4. Mix the fermentation supernatant with the prickle oil and homogenize to obtain the prickle extract.

3. The use according to claim 2, characterized in that: The supercritical CO2 extraction process parameters are: extraction pressure of 10-20 MPa, CO2 flow rate of 20-50 L / min, extraction temperature of 35-40° C., and extraction time of 1-3 h.

4. The use according to claim 2, characterized in that: In step T2, the enzyme used for enzymolysis is alkaline protease.

5. The use according to claim 4, characterized in that In the step T2, the enzymatic hydrolysis pH is 7.0-8.0, the enzyme mass fraction is 1.0-3.5%, the enzymatic hydrolysis time is 3-6 hours, and the material-liquid ratio is 1:20-35 g / mL.

6. The use according to claim 2, characterized in that: In the step T3, the amount of enzymatic hydrolysate added to the fermentation medium is 5-20%.

7. The use according to claim 6, characterized in that In step T3, the inoculation amount of the Yarrowia lipolytica is 1-3%; the fermentation conditions are: temperature 25-30° C., fermentation time 24-72 hours, shaking speed 150-300 r / min; centrifugation conditions are: 3000-5000 r / min, 5-10 min.

8. The use according to claim 7, characterized in that In the step T4, the fermentation supernatant and the prickle oil are mixed in a ratio of 10:1 to 5.

9. The Principia utilis extract according to any one of claims 1 to 8.

10. A shampoo having the effects of nourishing hair and reducing hair loss, characterized in that: The invention comprises the prickle fruit extract according to any one of claims 1 to 9.

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