A processing method of polygonatum sibiricum

Abstract

The present invention provides a processing method for polygonatum sibiricum, which relates to the technical field of traditional Chinese medicine processing. The processing method for polygonatum sibiricum mainly includes steps such as pre-drying polygonatum sibiricum, preliminary steaming, vacuum moistening treatment with a mixture of yellow rice wine and ice water bath, repeated steaming and sunning 3 times, pre-cooling with ice water bath, rapid cooling and placing at -80 °C, high-temperature steaming and then drying to a water content of 8% - 10%. The present invention overcomes the deficiencies of the prior art, shortens the steaming time, streamlines the processing procedures, reduces the toxicity of polygonatum sibiricum on the basis of effectively retaining the polysaccharide content in polygonatum sibiricum, improves the quality of the cut pieces, and further improves the production and processing efficiency.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine processing, and specifically relates to a processing method of Polygonatum sibiricum Redoute. Background Art

[0002] Polygonatum sibiricum Redoute is a herbaceous plant of the genus Polygonatum in the family Liliaceae, mainly distributed in the northeastern region. It tastes sweet and has a neutral nature, and has the effects of tonifying qi and nourishing yin, and moistening the lungs. However, raw Polygonatum sibiricum Redoute is irritating, and it will cause numbness in the mouth and tongue when taken, so generally Polygonatum sibiricum Redoute is taken after being processed. At present, the processing methods of Polygonatum sibiricum Redoute mainly include steaming, wine processing, nine-steaming and nine-boiling, etc. After processing, the polysaccharides, saponins and volatile substances in Polygonatum sibiricum Redoute have all changed significantly, its medicinal effect is enhanced, and the irritating toxicity is reduced, making it more suitable for human consumption.

[0003] The specific process parameters of Polygonatum sibiricum Redoute slices are not specified in the Chinese Pharmacopoeia (2020 Edition) and the processing specifications of each province. Most of them are processed according to prescriptions, resulting in uneven quality of processed products and affecting clinical efficacy. In addition, polysaccharides are the only quality control component of Polygonatum sibiricum Redoute specified in the Chinese Pharmacopoeia (2020 Edition). Therefore, at present, the processing requirements for Polygonatum sibiricum Redoute are often to retain the polysaccharide content in Polygonatum sibiricum Redoute as much as possible, and at the same time reduce the irritating components of Polygonatum sibiricum Redoute, that is, to reduce toxicity and increase efficacy to a certain extent. Therefore, it is necessary to further study and prepare Polygonatum sibiricum Redoute slices with high efficiency and low toxicity on the basis of the existing processing technology. Summary of the Invention

[0004] Aiming at the deficiencies of the existing technology, the present invention provides a processing method of Polygonatum sibiricum Redoute, which can effectively reduce the toxicity of Polygonatum sibiricum Redoute on the basis of retaining the polysaccharide content, improve the quality of the slices, and comprehensively reduce the steaming time and improve the production efficiency.

[0005] To achieve the above objectives, the present invention is realized through the following technical solutions:

[0006] A processing method of Polygonatum sibiricum Redoute, the processing method includes the following steps:

[0007] S1. Pretreatment of Polygonatum sibiricum Redoute: Wash and slice raw Polygonatum sibiricum Redoute, and dry it until the water content is 12%-15% to obtain pretreated Polygonatum sibiricum Redoute for standby;

[0008] S2. Preliminary steaming treatment: Place the pretreated Polygonatum sibiricum Redoute in a steamer, steam it at a temperature of 120-140°C for 4-6 hours, collect the juice of Polygonatum sibiricum Redoute during steaming, and obtain preliminarily steamed Polygonatum sibiricum Redoute for standby;

[0009] S3. Pre-cooling treatment: Mix the above-mentioned preliminarily steamed Polygonatum sibiricum Redoute with yellow rice wine, and then place it in an ice-water bath, seal it, evacuate and let it stand for 2-4 hours to obtain pre-cooled Polygonatum sibiricum Redoute for standby;

[0010] S4. Repeated steaming treatment: Place the pre-cooled polygonatum sibiricum in a steamer and steam it at a temperature of 120 - 140 °C for 1 - 2 hours, then dry it in the sun for 4 - 6 hours, and repeat the steaming and drying 3 times to obtain cooked polygonatum sibiricum slices for standby;

[0011] S5. Quick cooling treatment: Immerse the above-mentioned cooked polygonatum sibiricum in an ice-water bath for 10 - 20 minutes, then place it in an environment of -80 °C for quick cooling for 3 - 5 minutes, and then take it out to obtain quick-cooled polygonatum sibiricum slices for standby;

[0012] S6. High-temperature steaming treatment: Mix the above-mentioned quick-cooled polygonatum sibiricum slices with the polygonatum sibiricum juice in step S2 and mix them evenly, then place them in a steamer and steam them at a temperature of 150 - 170 °C for 1 - 2 hours, and then take them out and dry them until the water content is 8% - 10% to obtain processed polygonatum sibiricum decoction pieces.

[0013] Preferably, the thickness of the slices in step S1 is 5 - 10 mm.

[0014] Preferably, the drying temperature in step S1 is 40 - 50 °C.

[0015] Preferably, the steaming pressure in step S2 is 0.20 - 0.25 Mpa.

[0016] Preferably, the mass ratio of the preliminarily steamed polygonatum sibiricum to the yellow rice wine in step S3 is 10∶1 - 2.

[0017] Preferably, the vacuum pressure in step S3 is 20 - 40 KPa.

[0018] Preferably, the pressure is controlled at 0.2 - 0.25 Mpa during steaming in step S4.

[0019] Preferably, the drying temperature in step S6 is 40 - 50 °C.

[0020] Preferably, the steaming pressure in step S6 is 0.28 - 0.32 Mpa.

[0021] The present invention provides a processing method for polygonatum sibiricum, and the advantages compared with the prior art are as follows:

[0022] The processing method of polygonatum sibiricum in the present invention mainly includes slicing and drying it first, then steaming it preliminarily once, then adding yellow rice wine, immersing it in an ice-water bath, evacuating and moistening it, and then steaming and drying it 3 times and then performing a short-time quick cooling treatment and steaming and drying again. Compared with the traditional nine-steaming and nine-boiling method, the steaming times and steaming time are significantly shortened, the polysaccharide content of the final polygonatum sibiricum decoction pieces can be effectively guaranteed, the irritation of the polygonatum sibiricum decoction pieces can be reduced, and the overall medicinal effect can be improved. Brief Description of the Drawings

[0023] Figure 1This is a bar chart comparing the polysaccharide content of Polygonatum sibiricum Redoute slices and raw Polygonatum sibiricum Redoute in different groups in the embodiments of the present invention specification;

[0024] Figure 2 This is a bar chart comparing the effects of extracts of Polygonatum sibiricum Redoute slices and raw Polygonatum sibiricum Redoute in different groups on the proliferation of HK-2 and ICE-6 cells in the embodiments of the present invention specification;

[0025] Figure 3 This is a bar chart comparing the polysaccharide content of Polygonatum sibiricum Redoute slices under different rapid cooling times in the embodiments of the present invention specification;

[0026] Figure 4 This is a bar chart comparing the effects of extracts of Polygonatum sibiricum Redoute slices under different rapid cooling times on the proliferation of HK-2 and ICE-6 cells in the embodiments of the present invention specification. Detailed implementation manners

[0027] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention. Embodiment 1:

[0028] Preparation of Polygonatum sibiricum Redoute slices:

[0029] (1) Wash the raw Polygonatum sibiricum Redoute and cut it into thick slices of 5-10 mm, dry it at 45±5 °C until the water content is 12%-15% to obtain pretreated Polygonatum sibiricum Redoute for standby;

[0030] (2) Place the pretreated Polygonatum sibiricum Redoute in a steamer, steam it at 120 °C for 6 h under a pressure of 0.20 Mpa, collect the Polygonatum sibiricum Redoute juice during steaming, and obtain preliminarily steamed Polygonatum sibiricum Redoute for standby;

[0031] (3) Mix the above preliminarily steamed Polygonatum sibiricum Redoute and yellow rice wine according to a mass ratio of 10:1, then place it in an ice-water bath, seal and evacuate to a pressure of 20 Kpa and let it stand for 4 h to obtain pre-cooled Polygonatum sibiricum Redoute for standby;

[0032] (4) Place the pre-cooled Polygonatum sibiricum Redoute in a steamer, steam it at 120 °C for 2 h under a pressure of 0.2 Mpa, then dry it in the sun for 4 h, and repeat this step of steaming and drying 3 times to obtain cooked Polygonatum sibiricum Redoute slices for standby;

[0033] (5) Place the above cooked Polygonatum sibiricum Redoute in an ice-water bath for 10 min, then place it in an environment of -80 °C for rapid cooling for 5 min, and then take it out to obtain rapid-cooled Polygonatum sibiricum Redoute slices for standby;

[0034] (6) Mix the above-mentioned quickly cooled polygonatum slices with the above-mentioned polygonatum juice evenly, then place them in a steamer and steam at 150 °C under a pressure of 0.28 Mpa for 2 h, and then take them out and dry at 45 ± 5 °C until the water content is 8% - 10% to obtain the processed polygonatum slices. Example 2:

[0035] Preparation of polygonatum slices:

[0036] (1) Wash the fresh polygonatum and cut it into thick slices with a thickness of 5 - 10 mm, and dry at 45 ± 5 °C until the water content is 12% - 15% to obtain the pretreated polygonatum for standby;

[0037] (2) Place the pretreated polygonatum in a steamer and steam at 140 °C under a pressure of 0.25 Mpa for 4 h, collect the polygonatum juice during steaming, and obtain the preliminarily steamed polygonatum for standby;

[0038] (3) Mix the above-mentioned preliminarily steamed polygonatum with yellow rice wine according to a mass ratio of 10:2, and then place it in an ice-water bath, seal and evacuate to a pressure of 40 Kpa and let it stand for 2 h to obtain the pre-cooled polygonatum for standby;

[0039] (4) Place the pre-cooled polygonatum in a steamer and steam at 120 - 140 °C under a pressure of 0.25 Mpa for 1 - 2 h, then dry in the sun for 4 - 6 h, and repeat this steaming and drying step 3 times to obtain the cooked polygonatum slices for standby;

[0040] (5) Place the above-mentioned cooked polygonatum in an ice-water bath for 10 - 20 min, then place it in an environment of -80 °C and quickly cool for 3 min, and then take it out to obtain the quickly cooled polygonatum slices for standby;

[0041] (6) Mix the above-mentioned quickly cooled polygonatum slices with the above-mentioned polygonatum juice evenly, then place them in a steamer and steam at 170 °C under a pressure of 0.32 Mpa for 1 h, and then take them out and dry at 45 ± 5 °C until the water content is 8% - 10% to obtain the processed polygonatum slices.

[0042] Comparative Example 1:

[0043] Preparation of polygonatum slices:

[0044] (1) Wash the fresh polygonatum and cut it into thick slices with a thickness of 5 - 10 mm, and dry at 45 ± 5 °C until the water content is 12% - 15% to obtain the pretreated polygonatum for standby;

[0045] (2) Place the pretreated polygonatum in a steamer and steam at 120 °C under a pressure of 0.20 Mpa for 6 h, collect the polygonatum juice during steaming, and obtain the preliminarily steamed polygonatum for standby;

[0046] (3) Mix the above-mentioned preliminarily steamed polygonatum with yellow rice wine according to a mass ratio of 10:1, and then let it stand and moisten for 4 h to obtain the pre-cooled polygonatum for standby;

[0047] (4) Place the pre-cooled polygonatum sibiricum in a steamer and steam it at 120 °C for 2 h under a pressure of 0.2 Mpa, then sun-dry it for 4 h, and repeat this steaming and sun-drying process 3 times to obtain cooked polygonatum sibiricum slices for standby;

[0048] (5) After subjecting the above-mentioned cooked polygonatum sibiricum to an ice-water bath for 10 min, place it in an environment of -80 °C for rapid cooling and standing for 5 min, and then take it out to obtain rapidly cooled polygonatum sibiricum slices for standby;

[0049] (6) Mix the above-mentioned rapidly cooled polygonatum sibiricum slices with the above-mentioned polygonatum sibiricum juice evenly, then place them in a steamer and steam them at 150 °C for 2 h under a pressure of 0.28 Mpa, and then take them out and dry them at 45 ± 5 °C until the water content is 8% - 10% to obtain processed polygonatum sibiricum decoction pieces.

[0050] Comparative Example 2:

[0051] Preparation of polygonatum sibiricum decoction pieces:

[0052] (1) Wash the raw polygonatum sibiricum and cut it into thick slices of 5 - 10 mm, dry it at 45 ± 5 °C until the water content is 12% - 15% to obtain pre-treated polygonatum sibiricum for standby;

[0053] (2) Place the pre-treated polygonatum sibiricum in a steamer and steam it at 120 °C for 6 h under a pressure of 0.20 Mpa, collect the polygonatum sibiricum juice during steaming to obtain preliminarily steamed polygonatum sibiricum for standby;

[0054] (3) Mix the above-mentioned preliminarily steamed polygonatum sibiricum with yellow rice wine in a mass ratio of 10∶1, then place it in an ice-water bath, seal and evacuate to a pressure of 20 Kpa, and let it stand for 4 h to obtain pre-cooled polygonatum sibiricum for standby;

[0055] (4) Place the pre-cooled polygonatum sibiricum in a steamer and steam it at 120 °C for 2 h under a pressure of 0.2 Mpa, then sun-dry it for 4 h, and repeat this steaming and sun-drying process 3 times to obtain cooked polygonatum sibiricum slices for standby;

[0056] (5) Mix the above-mentioned cooked polygonatum sibiricum slices with the above-mentioned polygonatum sibiricum juice evenly, then place them in a steamer and steam them at 150 °C for 2 h under a pressure of 0.28 Mpa, and then take them out and dry them at 45 ± 5 °C until the water content is 8% - 10% to obtain processed polygonatum sibiricum decoction pieces.

[0057] Comparative Example 3:

[0058] Nine-steamed and nine-processed polygonatum sibiricum decoction pieces:

[0059] (1) Wash the raw polygonatum sibiricum and cut it into thick slices of 5 - 10 mm, dry it at 45 ± 5 °C until the water content is 12% - 15% to obtain pre-treated polygonatum sibiricum for standby;

[0060] (2) Put the sun-dried polygonatum sibiricum into a steamer and steam it at 120 °C for 3 h under a pressure of 0.20 Mpa, then take it out and sun-dry it naturally for 4 h to obtain preliminarily steamed polygonatum sibiricum for standby;

[0061] (3) Mix the above-mentioned preliminarily steamed polygonatum sibiricum with yellow rice wine in a mass ratio of 10:1, stir well, then put it into a steamer and steam at 120 °C under a pressure of 0.20 Mpa for 3 h, and then take it out and dry in the sun for 4 h;

[0062] (4) Repeat the operation in step (3) 8 times, and control the water content of polygonatum sibiricum at 8%-10% during the last drying in the sun to obtain the nine-steamed and nine-boiled polygonatum sibiricum slices.

[0063] Detection:

[0064] 1. Detect the polysaccharide content of polygonatum sibiricum slices under different processing technologies (determination is carried out with reference to the determination method of polygonatum sibiricum polysaccharide under polygonatum sibiricum in Part I of Chinese Pharmacopoeia 2020 Edition):

[0065] Select the same batch of raw polygonatum sibiricum, and prepare polygonatum sibiricum slices by using the above-mentioned Examples 1-2 and Comparative Examples 1-3 respectively. Detect the polygonatum sibiricum polysaccharide content of raw polygonatum sibiricum and each group of polygonatum sibiricum slices. The specific results are shown in Table 1 below:

[0066] Table 1

[0067]

[0068] 2. Detect the cytotoxicity of polygonatum sibiricum slices under different processing technologies. The specific operation method is as follows:

[0069] (1) Select the same batch of raw polygonatum sibiricum, and prepare polygonatum sibiricum slices by using the above-mentioned Examples 1-2 and Comparative Examples 1-3 respectively;

[0070] (2) Grind each group of polygonatum sibiricum slices and raw polygonatum sibiricum into powder respectively. Add methanol to each group of powders according to the material ratio of 1:10 (g / mL), perform ultrasonic treatment at 400 W for 30 min, filter, add methanol to the filter residue again according to the material ratio of 1:10 (g / mL), continue ultrasonic treatment at 400 W for 30 min, filter, combine the two filtrates, and freeze-dry to obtain the extracts of each group;

[0071] (3) Select human renal proximal tubular cells HK-2 and rat small intestinal crypt epithelial cells ICE-6 as experimental cells. Among them, HK-2 is placed in DMEM medium containing 10% fetal bovine serum (containing 100 U / mL penicillin and 0.1 g / L streptomycin) at 37 °C, 5% CO 2 and saturated humidity conditions for culture; ICE-6 is placed in DMEM medium (containing 100 μg / mL recombinant human insulin) at 37 °C, 5% CO 2 and saturated humidity conditions for culture.

[0072] (4) Use the MTT method to measure the cell proliferation rate:

[0073] Seed the above cells at a density of 1×105 Inoculate at a density of cells / mL into a 96-well plate (100 μL / well). After culturing for 24 h, add different extracts (0.5 mg / mL) to the experimental groups, with 5 replicate wells in each group. Additionally, set up a control group (without extract) and a blank group (blank medium), and continue culturing for 24 h. At the end of incubation, add 20 μL of MTT to each well and continue culturing for 4 h. Gently aspirate the supernatant, add 150 μL of DMSO to each well, and shake at low speed for 10 min to fully dissolve the crystals. Measure the absorbance of each well at 490 nm using an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the cell proliferation rate (Cell proliferation rate % = (Aexperimental group - Ablank group) / (Acontrol group - Ablank group) × 100%). The specific results are shown in Table 2 below:

[0074] Table 2

[0075]

[0076] As can be seen from the above table, the polygonatum sibiricum processed in Example 1 and Example 2 has lower cytotoxicity compared to the nine-steamed and nine-boiled polygonatum sibiricum.

[0077] 3. Prepare polygonatum sibiricum cut pieces by controlling different rapid cooling and standing times according to the method of Example 1, and detect the polysaccharide content of the final polygonatum sibiricum cut pieces at different rapid cooling times. The specific results are shown in Table 3 below:

[0078] Table 3

[0079]

[0080] Prepare extracts from polygonatum sibiricum cut pieces prepared with different rapid cooling and standing times, and detect their effects on the proliferation of HK-2 and ICE-6. The specific experimental procedure refers to Experiment 2 above. The results are shown in Table 4 below:

[0081] Table 4

[0082]

[0083] From the above detections, it can be seen that when the rapid cooling treatment is carried out for 3 - 5 min, the polygonatum sibiricum cut pieces have a relatively high cell proliferation rate and polysaccharide content. As the rapid cooling time increases, although the cell proliferation rate increases to some extent, the polysaccharide content decreases significantly. When the rapid cooling treatment time is less than 3 min, the cell proliferation rate is relatively low. Considering all factors, a rapid cooling treatment time of 3 - 5 min is the optimal time.

[0084] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for processing polygonatum, characterized in that: The processing method comprises the following steps: S1. Pretreatment of polygonatum: wash and slice the raw polygonatum, and dry it to a water content of 12%-15%, and prepare the pretreated polygonatum for later use; S2, preliminary steaming treatment: placing the pretreated polygonatum in a steamer, steaming it for 4-6 hours at a temperature of 120-140° C. and a pressure of 0.20-0.25 MPa, collecting the polygonatum juice during steaming, and obtaining preliminary steamed polygonatum for later use; S3, pre-cooling treatment: the above preliminary steamed polygonatum and yellow wine are mixed in a mass ratio of 10:1-2, and then sealed in an ice water bath, vacuumed and left to stand for 2-4 hours to obtain pre-cooled polygonatum for use; S4, repeated steaming treatment: placing the pre-cooled polygonatum in a steamer, steaming it at a temperature of 120-140° C. and a pressure of 0.2-0.25 MPa for 1-2 h, then drying it in the sun for 4-6 h, and repeating the steaming and drying for 3 times to obtain cooked polygonatum slices for later use; S5, rapid cooling treatment: treating the cooked polygonatum sibiricum in an ice water bath for 10-20 minutes, and then placing it in an environment below -80°C for rapid cooling for 3-5 minutes, and then taking out the rapidly cooled polygonatum sibiricum slices for use; S6, high-temperature steaming treatment: the above-mentioned quenched polygonatum slices are mixed with the polygonatum juice in the above-mentioned step S2, and mixed evenly, and then placed in a steamer and steamed at a temperature of 150-170°C and a pressure of 0.28-0.32Mpa for 1-2h, and then taken out and dried to a water content of 8%-10%, to obtain the prepared polygonatum slices.

2. The processing method according to claim 1, characterized in that: The thickness of the slices in step S1 is 5-10 mm.

3. The processing method according to claim 1, characterized in that: The drying temperature in step S1 is 40-50°C.

4. The processing method according to claim 1, characterized in that: The vacuum pressure in step S3 is 20-40 KPa.

5. The processing method according to claim 1, characterized in that: The drying temperature in step S6 is 40-50°C.

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