Traditional Chinese medicine composition for dispersing blood stasis and diminishing swelling and application of traditional Chinese medicine composition in medicine preparation

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for dispersing blood stasis and diminishing swelling and application thereof in medicine preparation. The traditional Chinese medicine composition for dispersing blood stasis and diminishing swelling is prepared from the following raw materials in parts by weight: caulis spatholobi, myrrh, salvia miltiorrhiza, leech, peach kernel, curcuma zedoary, frankincense, cassia twig and coix seed. The formula for dispersing blood stasis and diminishing swelling is simple in medicine flavor and reasonable in compatibility, the whole formula jointly achieves the effects of dispersing blood stasis and diminishing swelling, the medicine flavor fully achieves the synergistic effect, and the remarkable treatment effect on diseases such as lymphedema, deep vein thrombosis and lower limb varicose vein with edema is achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for removing blood stasis and relieving swelling and an application thereof in preparing medicines. Background Art

[0002] Lymphedema is not uncommon in clinical practice. Its main cause is damage to lymphatic vessels or lymph nodes, which leads to obstruction of lymphatic fluid return. Once normal lymphatic vessels lose their compensatory ability, protein-rich lymph fluid will accumulate in the body, leading to lymphedema. There are non-surgical and surgical methods for the treatment of lymphedema. Non-surgical methods include: manual drainage, baking and binding therapy, pressure therapy, functional exercise, skin care, drug therapy, etc. Although diuretics can reduce swelling in the short term, long-term use can lead to complications such as hypotension and electrolyte disorders. It is not recommended to use benzopyrone and coumarin, which can promote macrophages to decompose protein and gradually improve limb edema. It has been reported that after 6 months of treatment of lymphedema with benzopyrone drugs, edema can be relieved to a certain extent. It is currently generally used as an auxiliary drug for the treatment of limb lymphedema.

[0003] Deep vein thrombosis of the lower extremities is an abnormal coagulation of blood in the veins, which completely or partially blocks the blood vessels. It is a venous reflux disorder and belongs to the categories of "edema", "blood stasis", "blood stasis syndrome" and "pulse arthritis" in traditional Chinese medicine. It is one of the common serious complications after major orthopedic surgery, especially in lower extremity surgery or trauma. The detachment of deep vein thrombosis emboli in the lower extremities can cause pulmonary embolism and be fatal. In the later stage, it will be complicated by deep vein thrombosis syndrome in the lower extremities, which will cause lower extremity pain, swelling, superficial varicose veins, skin changes, etc. In severe cases, venous ulcers will occur.

[0004] How to find a drug that can effectively treat lymphedema and deep vein thrombosis of the lower limbs is an urgent problem that we need to solve. Summary of the invention

[0005] The present invention aims to provide a Chinese medicine composition for removing blood stasis and reducing swelling with simple medicinal flavor and reasonable compatibility, as well as a preparation method and application thereof. The Chinese medicine composition for removing blood stasis and reducing swelling has a significant therapeutic effect on diseases such as lymphedema, deep vein thrombosis, varicose veins of lower extremities with edema, etc.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] The first object of the present invention is to provide a Chinese medicine composition for removing blood stasis and reducing swelling.

[0008] The second object of the present invention is to provide a method for preparing the above-mentioned Chinese medicine composition for removing blood stasis and reducing swelling.

[0009] The third object of the present invention is to provide the use of the above-mentioned Chinese medicine composition for removing blood stasis and reducing swelling.

[0010] To achieve the first purpose, the technical solution adopted by the present invention is:

[0011] The invention provides a traditional Chinese medicine composition for removing blood stasis and relieving swelling. The traditional Chinese medicine composition is prepared from millet spatholobi, myrrh, salvia miltiorrhiza, leech, peach kernel, zedoaria, frankincense, cassia twig and coix seed.

[0012] Furthermore, the traditional Chinese medicine composition for removing blood stasis and reducing swelling is made of the following raw materials in parts by weight: 10-15 parts of Millettia reticulata, 10-15 parts of Myrrha, 10-15 parts of Salvia miltiorrhiza, 7-10 parts of Leech, 15-18 parts of Peach Kernel, 10-15 parts of Curcuma Atractylodes, 25-30 parts of Frankincense, 30-35 parts of Cinnamon Twig and 10-15 parts of Coix Seed.

[0013] Furthermore, the Chinese medicine composition for removing blood stasis and reducing swelling is made of the following raw materials in parts by weight: 12 parts of Millettia reticulata, 12 parts of Myrrha, 12 parts of Salvia miltiorrhiza, 8 parts of Leech, 16 parts of Peach Kernel, 12 parts of Curcuma Atractylodes, 28 parts of Frankincense, 32 parts of Cinnamon Twig and 12 parts of Coix Seed.

[0014] The Chinese medicine composition of the present invention also includes pharmaceutically acceptable excipients, which may include but are not limited to solvents, solubilizers, co-solvents, colorants, disintegrants, fillers, lubricants, wetting agents, stabilizers, flavoring agents, preservatives, suspending agents, coating materials, anti-adhesive agents, integrities, plasticizers, etc.

[0015] Furthermore, the dosage form of the Chinese medicine composition for removing blood stasis and reducing swelling is decoction, granules, tablets, capsules, mixtures, syrups, pills, gels, powders or pastes.

[0016] To achieve the second purpose, the technical solution adopted by the present invention is:

[0017] The preparation method of the Chinese medicine composition for removing blood stasis and relieving swelling is as follows: weighing raw materials according to the raw material ratio, adding water for decoction, and filtering to obtain a medicinal solution.

[0018] Furthermore, the preparation method of the present invention is as follows: weigh each raw medicinal material, crush it, soak it in water for 1-2 hours, decoct it twice, add water 5-8 times the total amount of medicinal materials each time, decoct it for 0.5-1.5 hours each time, and combine the decoctions.

[0019] Furthermore, the preparation method of the present invention is as follows: weigh each raw medicinal material, crush it, soak it in water for 1.5 hours, decoct it twice, add water 5 times the total amount of medicinal materials each time, decoct it for 1.2 hours each time, and combine the decoctions.

[0020] To achieve the third purpose, the technical solution adopted by the present invention is:

[0021] The present invention provides use of the blood stasis-removing and swelling-removing Chinese medicinal composition in preparing a medicine for preventing or removing blood stasis and swelling.

[0022] The pharmacological effects of the raw materials in the Chinese medicine composition for removing blood stasis and reducing swelling of the present invention are as follows:

[0023] Millettia reticulata: bitter, slightly sweet, warm in nature, enters the liver and kidney meridians, promotes blood circulation, replenishes blood, regulates menstruation, relaxes muscles and activates collaterals; used for irregular menstruation, dysmenorrhea, amenorrhea, rheumatic pain, numbness of hands and feet, paralysis of limbs, and anemia and sallow complexion.

[0024] Myrrh: pungent and bitter in taste, neutral in nature; enters the heart, liver, and spleen meridians, dissipates blood stasis and relieves pain, reduces swelling and promotes tissue regeneration; used to treat chest pain, heart pain, epigastric pain, dysmenorrhea, amenorrhea, postpartum blood stasis, abdominal pain due to masses, rheumatic pain, traumatic injuries, carbuncles, ulcers and other diseases.

[0025] Salvia miltiorrhiza: bitter in taste, slightly cold in nature, enters the heart and liver meridians, removes blood stasis and relieves pain, promotes blood circulation and unblocks menstruation, clears the heart and relieves restlessness; used for irregular menstruation, amenorrhea, dysmenorrhea, accumulation of masses, stabbing pain in the chest and abdomen, pain due to heat arthralgia, swelling and pain of sores, restlessness and insomnia; hepatosplenomegaly, angina pectoris.

[0026] Leeches: salty and bitter in taste, neutral in nature, enter the liver meridian, break up blood, remove blood stasis, and regulate menstruation; used for abdominal masses, blood stasis, amenorrhea, and injuries from falls.

[0027] Peach kernel: bitter and sweet in taste, neutral in nature, enters the heart, liver, and large intestine meridians, promotes blood circulation, removes blood stasis, moistens the intestines and promotes bowel movements; used for dysmenorrhea, blood stasis and amenorrhea, abdominal pain due to postpartum blood stasis, masses, injuries from falls, pain due to blood stasis, lung abscess, intestinal abscess, and constipation due to dry intestines.

[0028] Curcuma: It tastes pungent and bitter, is warm in nature, and can promote qi and resolve blood, eliminate accumulation and relieve pain; it is used to treat heart pain caused by blood and qi, food stagnation, abdominal distension and pain, blood stasis and amenorrhea, dysmenorrhea, tumors and lumps, and injuries from falls.

[0029] Frankincense: pungent and bitter in taste, warm in nature, enters the heart, liver, and spleen meridians, promotes blood circulation, relieves pain, reduces swelling and promotes tissue regeneration; used for chest pain, heart pain, epigastric pain, dysmenorrhea, amenorrhea, postpartum blood stasis, abdominal pain due to masses, rheumatic pain, tendon and vein spasm, traumatic injuries, carbuncles and ulcers.

[0030] Cinnamon twig: sweet in taste, slightly warm in nature, enters the lung and spleen meridians, tonifies Qi and raises Yang, consolidates the exterior and stops sweating, promotes diuresis and reduces swelling, promotes fluid and nourishes blood, relieves stagnation and arthritis, expels toxins and discharges pus, astringes sores and promotes tissue regeneration; used for fatigue due to Qi deficiency, poor appetite and loose stools, sinking of Qi in the middle, long-term diarrhea and prolapse of the anus, bloody stool and metrorrhagia, spontaneous sweating due to exterior deficiency, and edema due to Qi deficiency.

[0031] Job's tears: sweet and light in taste, cool in nature, enters the spleen, stomach, and lung meridians, promotes dampness and strengthens the spleen, relaxes muscles and removes numbness, clears away heat and discharges pus, detoxifies and disperses nodules; used for edema, athlete's foot, dribbling urination, diarrhea, rheumatic pain, muscle and vein spasms, flat warts, etc.

[0032] The prescription for removing blood stasis and relieving swelling of the invention has simple ingredients and reasonable compatibility, and the whole prescription has the effect of removing blood stasis and relieving swelling. The ingredients fully exert synergistic effects, and have significant therapeutic effects on diseases such as lymphedema, deep vein thrombosis, varicose veins of lower limbs with edema, etc.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] The Chinese medicine composition for removing blood stasis and reducing swelling of the present invention significantly reduces the hematocrit value, plasma viscosity and whole blood viscosity value of blood stasis model rats, and significantly improves blood rheology indexes. The Chinese medicine composition for removing blood stasis and reducing swelling of the present invention significantly inhibits the ear swelling of mice caused by xylene, and has a significant detumescence effect.

[0035] Animal experiments show that the Chinese medicine composition for removing blood stasis and reducing swelling of the present invention significantly relieves the symptoms of lymphedema. After the deep vein thrombosis model rats were treated with the Chinese medicine composition for removing blood stasis and reducing swelling of the present invention, the rats' APTT, TT and PT were significantly prolonged, which has a positive therapeutic effect on deep vein thrombosis. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 The hematocrit of rats in each group was compared. Different lowercase letters above the bar graph indicated significant differences among different groups (P<0.05).

[0037] Figure 2 Comparison of plasma viscosity among rats in each group. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05).

[0038] Figure 3 Comparison of low shear rate among rats in each group. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05).

[0039] Figure 4 Comparison of high shear rate among rats in each group. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05).

[0040] Figure 5 The ear swelling of mice in each group was compared. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05).

[0041] Figure 6 Comparison of tail diameters of mice in each group. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05).

[0042] Figure 7 Comparison of APTT of rats in each group. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05).

[0043] Figure 8Comparison of TT of rats in each group. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05).

[0044] Fig. 9 Comparison of PT among rats in each group. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05). DETAILED DESCRIPTION

[0045] The embodiments of the present invention will be described in detail below with reference to examples, but those skilled in the art will appreciate that the following examples are only used to illustrate the present invention and should not be construed as limiting the scope of the present invention.

[0046] Unless otherwise defined, all technical and scientific terms used in the present invention have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0047] Example 1

[0048] Millettia reticulata 12g, myrrh 12g, salvia miltiorrhiza 12g, leech 8g, peach kernel 16g, Curcuma 12g, frankincense 28g, cassia twig 32g and coix seed 12g.

[0049] The preparation method is as follows: weigh each raw medicinal material, crush it, soak it in water for 1.5 hours, decoct it twice, add 5 times the amount of water to the total amount of the medicinal material each time, decoct it for 1.2 hours each time, and combine the decoctions.

[0050] Example 2

[0051] Millettia reticulata 10g, myrrh 15g, salvia miltiorrhiza 15g, leech 7g, peach kernel 15g, Curcuma 15g, frankincense 25g, cassia twig 35g and coix seed 15g.

[0052] The preparation method is as follows: weigh each raw medicinal material, crush it, soak it in water for 2 hours, decoct it twice, add water 8 times the total amount of the medicinal materials each time, decoct it for 1 hour each time, and combine the decoctions.

[0053] Example 3

[0054] Millettia reticulata 15g, myrrh 10g, salvia miltiorrhiza 10g, leech 10g, peach kernel 18g, Curcuma 10g, frankincense 30g, cassia twig 30g and coix seed 10g.

[0055] The preparation method is as follows: weigh each raw medicinal material, crush it, soak it in water for 1.5 hours, decoct it twice, add 5 times the amount of water to the total amount of the medicinal material each time, decoct it for 0.5 hours each time, and combine the decoctions.

[0056] Example 4

[0057] Millettia reticulata 12g, myrrh 15g, salvia miltiorrhiza 10g, leech 8g, peach kernel 15g, Curcuma 10g, frankincense 25g, cassia twig 30g and coix seed 15g.

[0058] The preparation method is as follows: weigh each raw medicinal material, crush it, soak it in water for 1 hour, decoct it twice, add water 6 times the total amount of medicinal materials each time, decoct it for 1 hour each time, and combine the decoctions.

[0059] Example 5

[0060] Millettia reticulata 10g, myrrh 12g, salvia miltiorrhiza 12g, leech 10g, peach kernel 18g, Curcuma 12g, frankincense 30g, cassia twig 30g and coix seed 10g.

[0061] The preparation method is as follows: weigh each raw medicinal material, crush it, soak it in water for 1.5 hours, decoct it twice, add 5 times the amount of water to the total amount of the medicinal material each time, decoct it for 1.2 hours each time, and combine the decoctions.

[0062] Example 6

[0063] Millettia reticulata 10g, myrrh 10g, salvia miltiorrhiza 12g, leech 7g, peach kernel 16g, Curcuma 15g, frankincense 28g, cassia twig 30g and coix seed 12g.

[0064] The preparation method is as follows: weigh each raw medicinal material, crush it, soak it in water for 2 hours, decoct it twice, add 8 times the amount of water to the total amount of the medicinal material each time, decoct it for 1.5 hours each time, and combine the decoctions.

[0065] Example 7

[0066] Millettia reticulata 10g, myrrh 15g, salvia miltiorrhiza 12g, leech 10g, peach kernel 15g, Curcuma 12g, frankincense 25g, cassia twig 32g and coix seed 15g.

[0067] The preparation method is as follows: weigh each raw medicinal material, crush it, soak it in water for 1.5 hours, decoct it twice, add 5 times the amount of water to the total amount of the medicinal material each time, decoct it for 0.5 hours each time, and combine the decoctions.

[0068] Comparative Example 1

[0069] Myrrh 24g, Salvia miltiorrhiza 12g, Leech 8g, Peach Kernel 16g, Curcuma 12g, Frankincense 28g, Cinnamon Twig 32g and Coix Seed 12g.

[0070] Compared with Example 1, the difference is that Millettia spatholobi is not contained and the dosage of Myrrh is increased.

[0071] The preparation method is similar to that of Example 1.

[0072] Comparative Example 2

[0073] Millettia reticulata 12g, myrrh 12g, salvia miltiorrhiza 12g, leech 8g, peach kernel 22g, frankincense 34g, cassia twig 32g and coix seed 12g.

[0074] Compared with Example 1, the difference is that Curcuma zedoaria is not contained, and the dosage of peach kernel and frankincense is increased.

[0075] The preparation method is similar to that of Example 1.

[0076] Comparative Example 3

[0077] Millettia reticulata 12g, Salvia miltiorrhiza 24g, Leech 8g, Peach Kernel 22g, Curcuma 12g, Frankincense 28g, Cinnamon Twig 32g and Coix Seed 12g.

[0078] Compared with Example 1, the difference is that myrrh is not contained and the dosage of salvia miltiorrhiza is increased.

[0079] The preparation method is similar to that of Example 1.

[0080] Efficacy Example 1 Study on the effect of the Chinese medicine composition for removing blood stasis and reducing swelling on blood stasis model

[0081] 1. Experimental Methods

[0082] 56 SPF SD rats, weighing 160-200g, were randomly selected as blank control group, and the remaining rats were divided into model control group, embodiment 1 group, embodiment 2 group, comparative example 1 group, comparative example 2 group, comparative example 3 group according to the random number method, 8 rats in each group. Each group was gavaged with 8g / kg of the corresponding drug. Rats in the blank control group and model control group were gavaged with an equal volume of normal saline, at a dose of 10mL / kg, once a day, and continuously administered for 7d. On the 7th day, subcutaneous injection of epinephrine 0.08mL / 100g body weight was given twice, 4h apart, and the rats were immersed in ice water for 5min at the middle time point of the two injections.

[0083] 2. Index detection

[0084] The animals were not fed after treatment, and blood was drawn 1 hour after administration the next morning to measure hematocrit, whole blood viscosity, and plasma viscosity.

[0085] 3. Statistical methods

[0086] Graphpad Prism 9.0 software was used for data processing, and the measured data were expressed as mean ± standard deviation ( ±s), one-way analysis of variance was used for comparison among multiple groups, and SNK-q was used for pairwise comparison among groups. The difference was considered statistically significant when P < 0.05.

[0087] 4. Experimental Results

[0088] Compared with the blank control group, the whole blood viscosity and plasma viscosity of the rats in the model control group increased significantly, and the hematocrit value increased significantly, indicating that the model was successfully established.

[0089] Compared with the model control group, the hematocrit values ​​of rats in Example 1 and 2 were reduced. Compared with the comparative example 1-3 groups, there were significant differences in reducing the hematocrit values ​​in Example 1 and 2 groups. Figure 1 .

[0090] Compared with the model control group, the plasma viscosity and whole blood viscosity values ​​of rats in Example 1 and 2 were significantly reduced. Compared with Comparative Example 1-3 groups, there were significant differences in reducing plasma viscosity and whole blood viscosity values ​​in Example 1 and 2. Figure 2-Figure 4 .

[0091] The blood stasis-removing and swelling-reducing Chinese medicinal composition of the present invention reduces the hematocrit value, plasma viscosity and whole blood viscosity value of blood stasis model rats and significantly improves blood rheology indexes.

[0092] Efficacy Example 2 Experimental study on the effect of the Chinese medicine composition for removing blood stasis and reducing swelling on ear swelling in mice

[0093] 1. Test methods

[0094] 48 SPF Kunming mice, weighing 16-20 g, were randomly divided into a model control group, Example 1 group, Example 2 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group, with 8 mice in each group. Each group was gavaged with 14 g / kg of the corresponding drug. The model control group rats were gavaged with an equal volume of normal saline, once a day, for 7 consecutive days.

[0095] 2. Index detection

[0096] One hour after the last administration, 0.03 mL of xylene was applied to the right auricle of each mouse. The mice were killed 0.5 hours later, and the left and right auricles were punched out with a stainless steel punch with a diameter of 8 mm, weighed, and the swelling rate was calculated.

[0097] Ear swelling rate (%) = (right ear weight - left ear weight) / left ear weight × 100%.

[0098] 3. Statistical methods

[0099] Graphpad Prism 9.0 software was used for data processing, and the measured data were expressed as mean ± standard deviation ( ±s), one-way analysis of variance was used for comparison among multiple groups, and SNK-q was used for pairwise comparison among groups. The difference was considered statistically significant when P < 0.05.

[0100] 4. Experimental Results

[0101] Compared with the model control group, Example 1 and Example 2 significantly inhibited the ear swelling of mice caused by xylene. Compared with Comparative Examples 1-3, Example 1 and Example 2 showed significant differences in inhibiting the ear swelling of mice caused by xylene. Figure 5 .

[0102] The blood stasis-removing and swelling-removing Chinese medicinal composition of the present invention significantly inhibits the ear swelling of mice caused by xylene and has a significant swelling-removing effect.

[0103] Efficacy Example 3 Experimental study on the effect of the Chinese medicine composition for removing blood stasis and reducing swelling on lymphedema in mice

[0104] 1. Test methods

[0105] 24 SPF Kunming mice, weighing 16-20 g, were randomly divided into a blank control group and a modeling group according to a random number table.

[0106] The mice in the modeling group were anesthetized by intraperitoneal injection of 0.02 mL / g of 10% chloral hydrate, and the tail was disinfected with 75% ethanol. A circular incision was made 2 cm from the base of the tail, and the skin of the tail at the incision was about 2 mm deep. The skin and subcutaneous tissue of the tail were removed at the same time. Under a dissecting microscope, methylene blue injection was injected into the tip of the tail, and a color reaction was observed in the lymphatic vessels accompanying the skin excision site and the blood vessels on both sides of the tail. The exposed blue-stained lymphatic vessels were then removed with an electric knife, and the skin was fixed to the surrounding muscles with sutures. Erythromycin ointment was applied to the wound surface after surgery to prevent infection.

[0107] The mice with successful modeling were randomly divided into a model control group, an Example 1 group (administered with 14 g / kg of the decoction of Example 1), and an Example 2 group (administered with 14 g / kg of the decoction of Example 2), with 6 mice in each group. Each group of mice was intragastrically administered, and each of the blank control group and the model control group was intragastrically administered with 0.2 mL of normal saline each time, for 14 consecutive days, once a day. The changes in edema in the tail of the mice were observed and recorded.

[0108] 2. Index detection

[0109] On the 14th day of administration, the tail diameter of the mice was measured.

[0110] 3. Statistical methods

[0111] Graphpad Prism 9.0 software was used for data processing, and the measured data were expressed as mean ± standard deviation ( ±s), one-way analysis of variance was used for comparison among multiple groups, and SNK-q was used for pairwise comparison among groups. The difference was considered statistically significant when P < 0.05.

[0112] 4. Experimental Results

[0113] Compared with the blank control group, the tail diameter of the mice in the model control group increased significantly. Compared with the model control group, the tail diameter of the mice in Example 1 and 2 groups decreased significantly. Figure 6 .

[0114] The blood stasis-removing and swelling-removing Chinese medicinal composition of the present invention can significantly alleviate the symptoms of lymphedema.

[0115] Efficacy Example 4 Experimental study on the effect of the Chinese medicine composition for removing blood stasis and reducing swelling on the deep vein thrombosis model

[0116] 1. Establishment of rat deep vein thrombosis model

[0117] 24 SPF SD rats, weighing 180-220 g, were randomly selected from 8 blank control groups and divided into 2 groups according to the random number method, 8 in each group, namely the model control group and the Example 1 group (administered with 12 g / kg of the decoction of Example 1). Models were established in the model control group and the drug administration group.

[0118] After the rats were weighed and given 1% sodium pentobarbital intraperitoneal anesthesia, the rats' lower limbs were fixed with hip and knee flexion, and a 1 cm incision was made on the outside of the groin to separate the subcutaneous tissue of the rats. After the femur was exposed, the two ends of the femur were clamped with sterile hemostatic forceps and rotated in opposite directions until a snapping sound was heard. The integrity of the rat femur was checked for structural damage, which was determined to be a successful model. After the incision was sutured, the rats were raised normally without the use of antibiotics.

[0119] Each group was gavaged 3 days before modeling and 7 days after modeling. Rats in the blank control group and model control group were gavaged with an equal volume of normal saline, the dose was 10mL / kg, once a day, for 10 consecutive days.

[0120] 2. Index detection

[0121] On the 7th day after modeling, blood was collected from the eyeballs, and the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) were detected according to the kit method.

[0122] 3. Statistical methods

[0123] Graphpad Prism 9.0 software was used for data processing, and the measured data were expressed as mean ± standard deviation ( ±s), one-way analysis of variance was used for comparison among multiple groups, and SNK-q was used for pairwise comparison among groups. The difference was considered statistically significant when P < 0.05.

[0124] 4. Experimental Results

[0125] Compared with the blank control group, the APTT, TT and PT of rats in the model control group were significantly shortened, indicating that the model was successfully established.

[0126] Compared with the model control group, the APTT, TT and PT of rats in Example 1 group were significantly prolonged. Figure 7-9 .

[0127] After the deep vein thrombosis model rats were treated with the blood stasis and swelling-removing Chinese medicinal composition of the present invention, the rats' APTT, TT and PT were significantly prolonged, which has a positive therapeutic effect on deep vein thrombosis.

[0128] The present invention has been described in detail above by general description and specific embodiments, but it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made on the basis of not departing from the spirit of the present invention all belong to the scope of protection claimed by the present invention.

Claims

1. A Chinese medicine composition for removing blood stasis and reducing swelling, characterized in that: The traditional Chinese medicine composition for removing blood stasis and relieving swelling is prepared from the following raw materials in parts by weight: 10-15 parts of Millettia reticulata, 10-15 parts of Myrrha, 10-15 parts of Salvia miltiorrhiza, 7-10 parts of Leech, 15-18 parts of Peach Kernel, 10-15 parts of Curcuma Atractylodes, 25-30 parts of Frankincense, 30-35 parts of Cinnamon Twig and 10-15 parts of Coix Seed.

2. The Chinese medicine composition for removing blood stasis and reducing swelling according to claim 1, characterized in that: The Chinese medicine composition for removing blood stasis and relieving swelling is prepared from the following raw materials in parts by weight: 12 parts of Millettia reticulata, 12 parts of Myrrha, 12 parts of Salvia miltiorrhiza, 8 parts of Leech, 16 parts of Peach Kernel, 12 parts of Curcuma Atractylodes, 28 parts of Frankincense, 32 parts of Cinnamon Twig and 12 parts of Coix Seed.

3. The Chinese medicine composition for removing blood stasis and reducing swelling according to claim 1 or 2, characterized in that: The traditional Chinese medicine composition for removing blood stasis and relieving swelling also includes pharmaceutically acceptable excipients.

4. The Chinese medicine composition for removing blood stasis and reducing swelling according to claim 3, characterized in that: The traditional Chinese medicine composition for removing blood stasis and relieving swelling is a clinically acceptable dosage form.

5. The Chinese medicinal composition for removing blood stasis and reducing swelling according to claim 4, characterized in that: The dosage form of the Chinese medicine composition for removing blood stasis and relieving swelling is decoction, granules, tablets, capsules, mixtures, syrups, pills, gels, powders or pastes.

6. The Chinese medicine composition for removing blood stasis and reducing swelling according to claim 1 or 2, characterized in that: The preparation method of the Chinese medicine composition for removing blood stasis and relieving swelling is as follows: weigh each raw medicinal material, crush it, soak it in water for 1-2 hours, decoct it twice, add water 5-8 times the total amount of medicinal materials each time, decoct it for 0.5-1.5 hours each time, and combine the decoctions.

7. The Chinese medicinal composition for removing blood stasis and reducing swelling according to claim 6, characterized in that: The preparation method of the Chinese medicine composition for removing blood stasis and relieving swelling is as follows: weigh each raw medicinal material, crush it, soak it in water for 1.5 hours, decoct it twice, add water 5 times the total amount of medicinal materials each time, decoct it for 1.2 hours each time, and combine the decoctions.

8. Use of the blood stasis and swelling-removing Chinese medicinal composition according to claim 1 or 2 in the preparation of drugs for preventing or removing blood stasis and swelling.

9. Use of the Chinese medicinal composition for removing blood stasis and reducing swelling according to claim 1 or 2 in the preparation of a drug for treating lymphedema.

10. Use of the Chinese medicinal composition for removing blood stasis and reducing swelling according to claim 1 or 2 in preparing a drug for treating varicose veins with edema of lower extremities or deep vein thrombosis of lower extremities.

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