Nucleic acid extraction instrument and nucleic acid detection all-in-one machine

Abstract

The invention belongs to the field of molecular detection, and particularly relates to a nucleic acid extractor and a nucleic acid detection all-in-one machine, and the nucleic acid extractor comprises a bottom plate, and a sample carrier, a consumable carrier, a magnetic suction module, a pipetting module and a waste liquid removal module which are arranged on the bottom plate. The consumable carrier is used for placing pre-packaged consumables; the magnetic suction module is located below the consumable carrier and is provided with a magnetic suction piece capable of being close to or away from the reaction cavity; the pipetting module is used for transferring liquid between the sample carrier and the consumable carrier; the waste liquid removal module comprises a liquid suction pipeline and a liquid suction head arranged on the liquid suction pipeline, the liquid suction pipeline is used for being communicated with a waste liquid container, and the liquid suction head can move relative to the consumable carrier and can suck waste liquid in the reaction cavity into the waste liquid container through negative pressure. The waste liquid removal module is matched with the pre-packaged consumables, so that the time for removing the waste liquid, adding reagents and replacing the consumables can be greatly shortened, the extraction time is further shortened, and the nucleic acid extraction efficiency is improved.

Description

Technical Field

[0001] The present application relates to the field of molecular detection technology, and in particular to a nucleic acid extraction instrument and a nucleic acid detection all-in-one machine. Background Art

[0002] The substance of nucleic acid testing is the nucleic acid of the virus. Nucleic acid testing is to check whether there is nucleic acid of foreign invading viruses in the patient's respiratory samples, blood or feces to determine whether the patient is infected with the virus.

[0003] Nucleic acid detection requires the extraction of nucleic acid first, and then the extracted nucleic acid is tested. At present, the magnetic bead method nucleic acid extraction instruments on the market are divided into upper magnetic absorption method and lower magnetic absorption method according to the position of the magnetic beads. The upper magnetic absorption method is to set a magnetic absorption component above the reagent strip assembly, and the magnetic absorption component adsorbs the magnetic beads on the magnetic absorption component from the top of the reagent compartment and transfers them. The extraction time using the upper magnetic absorption method is relatively short, but there is a risk of contamination during the adsorption and transfer of magnetic beads; the lower magnetic absorption method is to set a magnetic absorption component at the bottom of the reagent strip assembly, and use the magnetic absorption component to adsorb the magnetic beads at the bottom of the reagent compartment, and remove the washing liquid in the reagent compartment from the top of the reagent compartment. Specifically, in the related technology, the washing liquid removal process is usually as follows: the liquid in the reagent compartment is absorbed by the pipette tip. After the pipette tip absorbs the liquid, the pipette tip and the liquid adsorbed therein are moved together to the top of the waste liquid tank for collecting waste liquid under the drive of the moving mechanism, and finally the liquid in the pipette tip is spit into the waste liquid tank. Although the possibility of contamination is low in the magnetic absorption method, the nucleic acid extraction time is relatively long due to the long time to remove waste liquid and add reagents during the extraction process. 。 Summary of the invention

[0004] The embodiments of the present application provide a nucleic acid extractor and a nucleic acid detection all-in-one machine, which aim to ensure anti-pollution capability while taking into account extraction efficiency.

[0005] To this end, according to one aspect of the present application, a nucleic acid extraction instrument is provided, comprising a base plate and a sample carrier, a consumable carrier, a magnetic suction module, a liquid transfer module and a waste liquid removal module arranged on the base plate;

[0006] The sample carrier is used to place the sample tube;

[0007] The consumable carrier is used to place prepackaged consumables, which include a consumable body and a reaction chamber and a reagent chamber arranged on the consumable body, wherein the reagent chamber contains a sample processing reagent;

[0008] The magnetic suction module is located below the consumable carrier, and the magnetic suction module has a magnetic suction part that can be close to or away from the reaction chamber;

[0009] The pipetting module is used to transfer liquid between the sample carrier and the consumable carrier;

[0010] The waste liquid removal module includes a liquid suction pipeline and a liquid suction head arranged on the liquid suction pipeline. The liquid suction pipeline is used to connect to the waste liquid container. The liquid suction head can move relative to the consumable carrier and can suck the waste liquid in the reaction chamber into the waste liquid container through negative pressure.

[0011] Optionally, the nucleic acid extractor further comprises a carrier base, which is slidably disposed on the bottom plate, and the sample carrier, consumable carrier and magnetic suction module are all disposed on the carrier base.

[0012] Optionally, the magnetic attraction module further includes a driving mechanism and a heating body, the magnetic attraction member and the heating body are both arranged on the driving mechanism, and the magnetic attraction member and the heating body can approach or move away from the reaction chamber under the action of the driving mechanism.

[0013] Optionally, the magnetic suction member and the heating body are arranged side by side, a plurality of pre-packaged consumables can be placed side by side on the consumable carrier, a plurality of magnetic suction grooves are arranged at intervals on the magnetic suction member, a plurality of heating grooves are arranged at intervals on the heating body, and the plurality of pre-packaged consumables, the plurality of magnetic suction grooves and the plurality of heating grooves correspond to each other one by one in the arrangement direction of the magnetic suction member and the heating body;

[0014] The magnetic attraction member and the heating body can move in the arrangement direction of the magnetic attraction member and the heating body and in the height direction of the reaction chamber under the action of the driving mechanism.

[0015] Optionally, a support frame and a driving assembly are provided on the base plate. The support frame is slidably arranged on the base plate along a first direction. The driving assembly is connected to the support frame and is used to drive the support frame to move in the first direction. The pipetting module and the waste liquid removal module are both arranged on the support frame. In the process of the pipetting module and the waste liquid removal module moving along the first direction with the support frame, they can be respectively opposite to the sample carrier and the consumables carrier in the height direction of the support frame.

[0016] Optionally, the driving assembly is arranged on a side of the base plate away from the support frame, and the driving end of the driving assembly is connected to an adapter, and the adapter is connected to an end of the support frame close to the base plate.

[0017] Optionally, the pipetting module includes a first linear module and a multi-channel pipetting assembly, the first linear module is arranged on a support frame, the multi-channel pipetting assembly is arranged on the first linear module and can move in the height direction of the support frame under the action of the first linear module, the multi-channel pipetting assembly has a plurality of mounting heads for detachably mounting gun tips, the plurality of mounting heads are arranged in sequence in a second direction, the multi-channel pipetting assembly can provide each gun tip with a force to absorb or spit out the solution, and the second direction intersects with the first direction and the height direction of the support frame.

[0018] Optionally, the waste liquid removal module also includes a second linear module, which is arranged on the support frame. The liquid suction pipeline is arranged on the second linear module and can move in the height direction of the support frame under the action of the second linear module. The liquid suction pipeline has a plurality of joints arranged in sequence in the second direction, and each joint is detachably connected to a liquid suction head, and the liquid suction head includes a gun head. The second direction intersects with the first direction and the height direction of the support frame.

[0019] Optionally, the consumable carrier further comprises a gun tip placement position for placing the gun tip.

[0020] According to another aspect of the present application, a nucleic acid detection integrated machine is provided, including a PCR detector and the above nucleic acid extraction instrument, and the PCR detector is arranged on a bottom plate.

[0021] The beneficial effects of the nucleic acid extractor and nucleic acid detection all-in-one provided by the present application are as follows: compared with the prior art, the nucleic acid extractor of the present application arranges the magnetic suction module below the consumable carrier, and the magnetic suction part can be used to adsorb the magnetic beads in the reaction chamber to the inner wall of the reaction chamber during the nucleic acid extraction process, and then the liquid in the reaction chamber is removed from the top of the reaction chamber by the waste liquid removal module. Since the magnetic suction module located below the consumable carrier is used to adsorb the magnetic beads to the bottom of the reaction chamber, compared with the upper adsorption extraction method, there is no transfer of magnetic beads outside the reaction chamber, so that the nucleic acid extractor has the advantage of low possibility of contamination during the nucleic acid extraction process; at the same time, the consumable carrier is used to place consumables pre-packaged with sample processing reagents, and the waste liquid removal module can directly suck the waste liquid in the reaction chamber during the extraction process into the waste liquid container by negative pressure suction, eliminating the steps of absorbing, transferring and spitting out the waste liquid. The waste liquid removal module cooperates with the pre-packaged consumables to greatly reduce the time for removing waste liquid, adding reagents and replacing consumables, thereby shortening the extraction time, improving the nucleic acid extraction efficiency, and ensuring the detection accuracy and efficiency of the nucleic acid detection all-in-one. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0023] in:

[0024] Figure 1 It is a schematic diagram of the overall structure of a nucleic acid extraction instrument shown in one embodiment of the present application;

[0025] Figure 2It is a schematic diagram of the connection between the bottom plate, the sample carrier, the consumable carrier, the magnetic suction module and the carrier base of the nucleic acid extraction instrument shown in one embodiment of the present application;

[0026] Figure 3 It is a structural schematic diagram of a magnetic suction module in a nucleic acid extraction instrument shown in an embodiment of the present application;

[0027] Figure 4 It is a structural schematic diagram of the overall structure of the nucleic acid extraction instrument shown in one embodiment of the present application from another perspective;

[0028] Figure 5 It is a structural schematic diagram of a liquid transfer module in a nucleic acid extraction instrument shown in an embodiment of the present application;

[0029] Figure 6 yes Figure 5 A schematic structural diagram of the pipetting module from another perspective;

[0030] Figure 7 It is a structural schematic diagram of a waste liquid removal module in a nucleic acid extraction instrument shown in one embodiment of the present application;

[0031] Figure 8 is a schematic structural diagram of another perspective shown in one embodiment of the present application;

[0032] Fig. 9 It is a schematic diagram of the overall structure of an all-in-one nucleic acid detection machine shown in one embodiment of the present application.

[0033] Description of main component symbols:

[0034] 10. Nucleic acid extraction instrument;

[0035] 20. PCR detector;

[0036] 30. Sample tube;

[0037] 40. Pre-packaged consumables; 41. Reaction chamber;

[0038] 50. Gun head;

[0039] 100, bottom plate; 101, strip-shaped through hole;

[0040] 200, sample carrier;

[0041] 300, consumables carrier;

[0042] 400, carrier base;

[0043] 500, magnetic module; 510, driving mechanism; 511, first linear module; 512, second linear module; 520, magnetic member; 521, magnetic slot; 530, heating element; 531, heating slot;

[0044] 600, support frame; 610, support plate; 620, mounting plate;

[0045] 700, driving assembly; 710, motor; 720, lead screw; 730, transmission nut; 740, connecting rod; 750, adapter;

[0046] 800, pipetting module; 810, first linear module; 820, multi-channel pipetting assembly; 821, mounting head;

[0047] 900, waste liquid removal module; 910, second linear module; 920, liquid suction pipeline; 921, connector. DETAILED DESCRIPTION

[0048] In order to facilitate the understanding of the present application, the present application will be described more comprehensively with reference to the relevant drawings below. The preferred embodiments of the present application are provided in the drawings. However, the present application can be implemented in many other different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present application more thorough and comprehensive.

[0049] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

[0050] It should be understood that the terms "length", "width", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0051] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0052] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used herein in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application.

[0053] It should also be noted that, in the embodiments of the present application, the same figure mark is used to represent the same component or the same part. For the same parts in the embodiments of the present application, the figure may only mark one of the parts or components as an example. It should be understood that the figure mark also applies to other identical parts or components.

[0054] According to one aspect of the present application, an embodiment of the present application provides a nucleic acid extraction instrument, such as Figure 1 As shown, the nucleic acid extractor 10 includes a base plate 100 and a sample carrier 200 , a consumable carrier 300 , a magnetic suction module 500 , a pipetting module 800 and a waste liquid removal module 900 disposed on the base plate 100 .

[0055] The sample carrier 200 is used to place the sample tube 30; the consumable carrier 300 is used to place the pre-packaged consumable 40, the pre-packaged consumable 40 includes a consumable body and a reaction chamber 41 and a reagent chamber arranged on the consumable body, the reagent chamber contains a sample processing reagent; the magnetic suction module 500 is located below the consumable carrier 300, and the magnetic suction module 500 has a magnetic suction member 520 that can be close to or away from the reaction chamber 41, and the magnetic suction member 520 has an adsorption state that adsorbs the magnetic beads in the reaction chamber 41 to the inner wall of the reaction chamber. The pipetting module 800 is used to transfer liquid between the sample carrier 200 and the consumable carrier 300; the waste liquid removal module 900 includes a pipetting pipeline 920 and a pipetting head (not shown in the figure) arranged on the pipetting pipeline 920, the pipetting pipeline 920 is used to connect to the waste liquid container (not shown in the figure), the pipetting head can move relative to the consumable carrier 300, and can suck the waste liquid in the reaction chamber 41 into the waste liquid container through negative pressure.

[0056] In the embodiment of the present application, the nucleic acid extractor 10 arranges the magnetic suction module 500 below the consumables carrier 300, and uses the magnetic suction member 520 to adsorb the magnetic beads in the reaction chamber 41 to the inner wall of the reaction chamber 41 during the nucleic acid extraction process, and then removes the liquid in the reaction chamber 41 from the top of the reaction chamber 41 through the waste liquid removal module 900. Since the magnetic suction module 500 located below the consumables carrier 300 is used to adsorb the magnetic beads to the bottom of the reaction chamber 41, compared with the upper adsorption extraction method, there is no transfer of the magnetic beads outside the reaction chamber 41, so that The nucleic acid extractor 10 has the advantage of low possibility of contamination during the nucleic acid extraction process; at the same time, the consumables carrier 300 is used to place pre-packaged consumables 40, and the waste liquid removal module 900 can directly suck the waste liquid in the reaction chamber 41 during the extraction process into the waste liquid container by negative pressure suction, eliminating the steps of absorbing, transferring and spitting out the waste liquid. The waste liquid removal module 900 cooperates with the pre-packaged consumables 40 on the consumables carrier 300, which can greatly reduce the time for adding reagents, replacing consumables and removing waste liquid, thereby shortening the extraction time and improving the efficiency of nucleic acid extraction.

[0057] During actual use, the sample tube 30 containing the sample solution is placed on the sample carrier 200, the prepackaged consumables 40 are placed on the consumable carrier 300, and the pipetting module 800 transfers the sample solution in the sample tube 30 to the reaction chamber 41 of the prepackaged consumables 40. The pipetting module 800 then transfers the lysate in the corresponding reagent chamber on the prepackaged consumables 40 to the reaction chamber 41 to lyse the cells and release the nucleic acids. Then, the pipetting module 800 transfers the magnetic bead suspension in the corresponding reagent chamber on the prepackaged consumables 40 to the reaction chamber 41, and uses the magnetic beads to absorb the nucleic acids. The magnetic suction component 520 of the magnetic suction module 500 is close to the reaction chamber 41, providing an external magnetic field to absorb the magnetic beads adsorbed with nucleic acids on the inner wall of the reaction chamber 41, and the waste liquid removal module 900 removes the lysate in the reaction chamber 41 through the liquid suction head. Then, the liquid transfer module 800 transfers the washing liquid in the corresponding reagent cavity on the pre-packaged consumable 40 to the reaction cavity 41, removes the external magnetic field first, mixes the magnetic beads with the washing liquid, washes away impurities, and then provides an external magnetic field to adsorb the magnetic beads on the inner wall of the reaction cavity 41. The waste liquid removal module 900 removes the washing liquid in the reaction cavity 41 through the liquid pipetting head. Finally, the liquid transfer module 800 transfers the eluate in the corresponding reagent cavity on the pre-packaged consumable 40 to the reaction cavity 41, removes the external magnetic field, mixes the magnetic beads with the eluate, and the bound nucleic acid can be separated from the magnetic beads and mixed into the eluate. The supernatant is taken to obtain the purified nucleic acid.

[0058] As can be seen from the above, due to the use of pre-packaged consumables 40, the nucleic acid extractor 10 does not need to frequently replace consumables during the process of extracting nucleic acids, which reduces the time for adding reagents. The pre-packaged consumables 40 can complete the steps of lysis, washing, elution, etc., which is easy to use. The waste liquid removal module 900 directly pumps the waste liquid (i.e., the used lysis solution and washing solution) in the reaction chamber 41 to the waste liquid container by negative pressure suction, eliminating the steps of suction and transfer. By pre-packaging consumables and reagents and directly sucking out the waste liquid, the time for adding reagents, changing consumables and removing waste liquid is greatly reduced, ensuring anti-pollution ability while taking into account extraction efficiency.

[0059] It should be noted that the pre-packaged consumable 40 is provided with a plurality of reagent cavities, which respectively contain lysis solution, magnetic bead suspension, washing solution and elution solution. The reaction chamber 41 and the plurality of reagent cavities are arranged in a straight line on the consumable body. The pre-packaged consumable 40 can adopt the existing technology, which will not be repeated here.

[0060] It is understandable that the method for achieving negative pressure for the pipette head on the waste liquid removal module 900 can be achieved by connecting the pipette line 920 to a waste liquid container with negative pressure inside, or by setting a vacuum pump between the pipette line 920 and the waste liquid container, which is not limited here.

[0061] In one embodiment, if Figure 1-Figure 2 As shown, the nucleic acid extractor 10 further includes a carrier base 400 , which is slidably disposed on the bottom plate 100 , and the sample carrier 200 , the consumable carrier 300 and the magnetic suction module 500 are all disposed on the carrier base 400 .

[0062] Through the above arrangement, the push-pull carrier base 400 can drive the sample carrier 200, the consumable carrier 300 and the magnetic suction module 500 located thereon to move together, so that the sample carrier 200 and the consumable carrier 300 can be staggered with the pipetting module 800 and the waste liquid removal module 900 in the vertical direction, thereby facilitating the placement of sample tubes 30 and pre-packaged consumables 40 on the sample carrier 200 and the consumable carrier 300 respectively.

[0063] Furthermore, a limit piece for limiting the push-pull limit position of the carrier base 400 is also provided on the base, and a handle is also provided on the carrier base 400, which facilitates the operator to apply force when pushing and pulling the carrier base 400.

[0064] In one embodiment, if Figure 2-Figure 3 As shown, the magnetic attraction module 500 also includes a driving mechanism 510 and a heating body 530 . The magnetic attraction member 520 and the heating body 530 are both arranged on the driving mechanism 510 , and the magnetic attraction member 520 and the heating body 530 can approach or move away from the reaction chamber 41 under the action of the driving mechanism 510 .

[0065] Through the above arrangement, the magnetic attraction component also has a heating function, and can be used in situations where the reaction reagents need to be heated.

[0066] In addition, the magnetic suction component 520 and the heating module are arranged on the same driving mechanism 510, and a driving mechanism 510 is used to control the movement of the magnetic suction module and the heating body 530 in a three-dimensional space to perform magnetic suction operations and heating operations on the reaction chamber 41, thereby reducing the number of driving mechanisms 510 and rationally utilizing the space at the same time. The magnetic suction component 520 and the heating module move synchronously to ensure the synergy of the heating and magnetic suction functions during the magnetic suction extraction process, improve the detection sensitivity, optimize the overall structure of the device, reduce the space occupancy rate of the device, and facilitate the miniaturization design of the entire nucleic acid extractor 10.

[0067] In a specific embodiment, please continue to refer to Figure 2 and Figure 3 The magnetic member 520 and the heating body 530 are arranged side by side, and a plurality of pre-packaged consumables 40 can be placed side by side on the consumable carrier 300. A plurality of magnetic grooves 521 are arranged at intervals on the magnetic member 520, and a plurality of heating grooves 531 are arranged at intervals on the heating body 530. The plurality of pre-packaged consumables 40, the plurality of magnetic grooves 521 and the plurality of heating grooves 531 correspond one to one in the arrangement direction of the magnetic member 520 and the heating body 530 respectively; the magnetic member 520 and the heating body 530 can move in the arrangement direction of the magnetic member 520 and the heating body 530, and in the height direction of the reaction chamber 41 under the action of the driving mechanism 510.

[0068] Through the above arrangement, the magnetic attraction component 520 and the heating body 530 in the magnetic attraction module 500 can respectively and simultaneously perform magnetic attraction and heating operations on multiple reaction chambers 41 under the action of the driving mechanism 510. In conjunction with the pipetting module 800 with a multi-channel pipetting component 820 and the waste liquid removal module 900 with multiple pipette heads, multiple nucleic acid extraction operations can be realized simultaneously. The highly integrated design greatly reduces the size of the entire machine. By way of example, in the figure, eight sample tubes 30 are placed side by side on the sample carrier 200, and eight pre-packaged consumables 40 are placed side by side on the consumable carrier 300. Eight magnetic attraction grooves 521 are shown on the magnetic attraction component 520, and eight heating grooves 531 are shown on the heating body 530.

[0069] For the convenience of explaining the directions, an XYZ three-dimensional coordinate system is established in the figure, wherein the arrangement direction of the magnetic member 520 and the heating body 530 is the direction where the X axis is located. Multiple magnetic suction grooves 521 are arranged on the magnetic member 520 at intervals in the direction where the Y axis is located, multiple heating grooves 531 are arranged on the heating body 530 at intervals in the direction where the Y axis is located, multiple pre-packaged consumables 40 are arranged side by side on the consumable carrier 300 in the direction where the Y axis is located, and multiple sample tubes 30 are arranged side by side on the sample carrier 200 in the direction where the Y axis is located. The height direction of the sample tube 30 and the reaction chamber 41 is the direction where the Z axis is located.

[0070] Specifically, the magnetic attraction member 520 may be a permanent magnet or an electromagnet, the heating body 530 may include a heating portion (such as an electric heating film or electric heating wire) and a heat conducting portion (such as a metal block) connected to each other, and the heating groove 531 is arranged on the heat conducting portion. The magnetic attraction groove 521 and the heating groove 531 are both V-shaped grooves to better fit with the reaction chamber 41 with a V-shaped bottom to ensure the magnetic attraction and heating effects.

[0071] In a more specific embodiment, Figure 2-Figure 3 As shown, the driving mechanism 510 includes a first linear module 511 and a second linear module 512. The second linear module 512 is arranged on the first linear module 511. The magnetic component 520 and the heating body 530 are both arranged on the second linear module 512. The magnetic component 520 and the heating body 530 can move in the arrangement direction of the magnetic component 520 and the heating body 530 under the action of the first linear module 511, and move in the height direction of the reaction chamber 41 under the action of the second linear module 910.

[0072] It can be understood that by moving in the arrangement direction of the magnetic attraction member 520 and the heating body 530, the magnetic attraction operation and the heating operation can be switched; by moving in the height direction of the reaction chamber 41, the up and down movement can be achieved to fit in or away from the bottom of the reaction chamber 41. Through the above arrangement, the driving mechanism 510 only needs to perform driving in two directions, with a simple structure and convenient control.

[0073] The first linear module 511 and the second linear module 512 may both be synchronous belt linear modules, ball screw linear modules, gear rack slide modules, or even pneumatic cylinders or electric cylinders.

[0074] In one embodiment, if Figure 1 and Figure 4As shown, a support frame 600 and a driving assembly 700 are provided on the base plate 100. The support frame 600 is slidably provided on the base plate 100 along a first direction. The driving assembly 700 is connected to the support frame 600 and is used to drive the support frame 600 to move in the first direction. The pipetting module 800 and the waste liquid removal module 900 are both provided on the support frame 600. In the process of moving along the first direction with the support frame 600, the pipetting module 800 and the waste liquid removal module 900 can be respectively opposite to the sample carrier 200 and the consumable carrier 300 in the height direction of the support frame 600.

[0075] The pipetting module 800 and the waste liquid removal module 900 are arranged on the same support frame 600, and the driving assembly 700 is used to drive the support frame 600 to move in the first direction. On the one hand, the pipetting module 800 and the waste liquid removal module 900 are arranged on the bottom plate 100 with the support frame 600 as a carrier at the same time, and the structure is simple and compact; on the other hand, after the driving assembly 700 drives the support frame 600 to move, the pipetting module 800 and the waste liquid removal module 900 are driven to be opposite to the sample carrier 200 and the consumable carrier 300 respectively in the height direction of the support frame 600, and the pipetting module 800 and the waste liquid removal module 900 only need to perform corresponding lifting operations, which is conducive to simplifying the driving parts of the pipetting module 800 and the waste liquid removal module 900, and at the same time, it can also avoid the situation that the pipetting module 800 and the waste liquid removal module 900 are easily subject to motion interference during the independent movement of each other in space.

[0076] Specifically, the support frame 600 includes two support plates 610 and two mounting plates 620. The two support plates 610 are respectively arranged on opposite sides of the base plate 100 in the first direction. The two mounting plates 620 are spaced apart between the two support plates 610 in the first direction. The two mounting plates 620 serve as mounting carriers of the pipetting module 800 and the waste liquid removal module 900 respectively. The ends of the two support plates 610 close to the base plate 100 can be connected to the base plate 100 respectively through linear guide rails extending along the first direction, so as to realize that the support frame 600 is slidably arranged on the base plate 100 along the first direction.

[0077] In the XYZ three-dimensional coordinate system in the figure, the first direction is the direction of the X-axis, the second direction in the following embodiments is the direction of the Y-axis, a plurality of prepackaged consumables 40 and a plurality of sample tubes 30 are respectively arranged in sequence in the direction of the Y-axis, and the height direction of the support frame 600 is the direction of the Z-axis.

[0078] In a specific embodiment, Figure 4 As shown, the driving assembly 700 is disposed on a side of the base plate 100 away from the support frame 600 , and the driving end of the driving assembly 700 is connected to an adapter 750 , and the adapter 750 is connected to an end of the support frame 600 close to the base plate 100 .

[0079] Since the sample carrier 200, the consumable carrier 300, the magnetic suction module 500, the pipetting module 800 and the waste liquid removal module 900 are all arranged on the top surface of the base plate 100, the drive assembly 700 is arranged on the side of the base plate 100 away from the support frame 600 (that is, the bottom surface of the base plate 100). This can avoid the drive assembly 700 from interfering with the structure arranged on the top surface of the base plate 100. At the same time, the space is reasonably planned, the overall structure of the equipment is optimized, and the space occupancy rate of the equipment is reduced.

[0080] In one implementation, the driving assembly 700 includes a motor 710, a lead screw 720, a transmission nut 730 and a connecting rod 740. The lead screw 720 is rotatably arranged on the base plate 100 around its own axis, and the axial direction of the lead screw 720 is parallel to the first direction. The motor 710 is fixed to the base plate 100 and is drivingly connected to the lead screw 720, and is used to drive the lead screw 720 to rotate around its own axis. The transmission nut 730 is threadedly connected to the lead screw 720, so that when the lead screw 720 rotates around its own axis, the transmission nut 730 can be driven to move relative to the base plate 100 along the first direction. The connecting rod 740 is fixedly connected to the transmission nut 730. The base plate 100 is provided with a strip through hole 101 that passes through the base plate 100 from top to bottom, and the length direction of the strip through hole 101 is parallel to the first direction. The adapter 750 is arranged in the strip through hole 101, and one end of the adapter 750 is connected to the support frame 600, and the other end is connected to the connecting rod 740.

[0081] The drive assembly 700 uses a screw pair formed by a screw 720 and a drive nut 730 to drive the support frame 600 to move relative to the base, which has a simple structure, high motion accuracy and is easy to control. Of course, in other implementations, the drive assembly 700 can also use other drive structures, such as a belt drive structure, a gear rack drive structure, etc., as long as it can achieve the drive support frame 600 to move relative to the base along the first direction, which is not limited here.

[0082] In a specific embodiment, Figure 1 and Figure 5-Figure 6 As shown, the pipetting module 800 includes a first linear module 810 and a multi-channel pipetting assembly 820. The first linear module 810 is arranged on the support frame 600. The multi-channel pipetting assembly 820 is arranged on the first linear module 810 and can move in the height direction of the support frame 600 under the action of the first linear module 810. The multi-channel pipetting assembly 820 has a plurality of mounting heads 821 for detachably mounting gun tips (not shown in the figure). The plurality of mounting heads 821 are arranged in sequence in the second direction. The multi-channel pipetting assembly 820 can provide each gun tip with a force to absorb or spit out the solution (i.e., generate a negative pressure to make the gun tip absorb the liquid or a positive pressure to make the gun tip discharge the liquid). The second direction intersects with the first direction and the height direction of the support frame 600.

[0083] When the pipetting module 800 transfers liquid between the sample carrier 200 and the consumable carrier 300, the following steps are required: 1. The driving assembly 700 drives the support frame 600 to drive the pipetting module 800 to move along the first direction to be opposite to the sample carrier 200 or the consumable carrier 300 in the height direction of the support frame 600; 2. The first linear module 810 drives the multi-channel pipetting assembly 820 and the gun head (not shown in the figure) located thereon to descend and insert into the corresponding cavity or sample tube 30 to extract liquid; 3. The first linear module 81 0 drives the multi-channel pipetting assembly 820 and the gun tip located thereon to be lifted to a corresponding height; 4. The driving assembly 700 drives the support frame 600 to drive the pipetting module 800 to move along the first direction to be opposite to the sample carrier 200 or the consumable carrier 300 in the height direction of the support frame 600; 5. The first linear module 810 drives the multi-channel pipetting assembly 820 and the gun tip located thereon to descend and spit out the liquid in the gun tip; 6. The first linear module 810 drives the multi-channel pipetting assembly 820 and the gun tip located thereon to be lifted to a corresponding height.

[0084] Specifically, the first linear module 810 can be a synchronous belt linear module, a ball screw linear module, a gear rack slide module, or even a cylinder or an electric cylinder, etc., which is not limited here.

[0085] Furthermore, the pipetting module 800 also includes a gun tip detaching mechanism, which is connected to the multi-channel pipetting assembly 820 and is at least partially movable relative to the mounting head 821 to detach the gun tip from the mounting head 821 .

[0086] In a specific embodiment, Figure 1 and Figure 7-Figure 8 As shown, the waste liquid removal module 900 also includes a second linear module 910, which is arranged on the support frame 600. The liquid suction pipeline 920 is arranged on the second linear module 910 and can move in the height direction of the support frame 600 under the action of the second linear module 910. The liquid suction pipeline 920 has a plurality of joints 921 arranged in sequence in the second direction, and each joint 921 is detachably connected to a liquid suction head, and the liquid suction head includes a gun head. The second direction intersects with the first direction and the height direction of the support frame 600.

[0087] When the waste liquid removal module 900 removes the waste liquid in the reaction chamber 41 of the pre-packaged consumables 40, the following steps are required: 1. The driving assembly 700 drives the support frame 600 to drive the waste liquid removal module 900 to move along the first direction to be opposite to the reaction chamber 41 in the height direction of the support frame 600; 2. The second linear module 910 drives the liquid suction pipeline 920 and the gun tip located thereon (not shown in the figure) to descend and insert into the reaction chamber 41 to suck the waste liquid into the waste liquid container; 3. The second linear module 910 drives the liquid suction pipeline 920 and the gun tip located thereon to be lifted to the corresponding height.

[0088] Specifically, the second linear module 910 can be a synchronous belt linear module, a ball screw linear module, a gear rack slide module, or even a cylinder or an electric cylinder, etc., which is not limited here.

[0089] Furthermore, the waste liquid removal module 900 also includes a gun tip detaching mechanism, which is connected to the liquid suction pipeline 920 and is at least partially movable relative to the connector 921 to detach the gun tip from the connector 921 .

[0090] It is understandable that in this embodiment, the plurality of joints 921 on the liquid pipette 920 are detachably connected to a gun head, and after each waste liquid removal, only the gun head needs to be replaced. Of course, in other embodiments, the liquid pipette head may also be a sampling needle, and in conjunction with a sampling needle cleaning mechanism, the sampling needle may be cleaned after each waste liquid removal.

[0091] In some embodiments, Figure 1 and Figure 2 As shown, the consumable carrier 300 also has a tip placement position for placing the tip 50. The tip placement position is used to place the tip 50 used by the pipetting module 800 and the waste liquid removal module 900.

[0092] According to another aspect of the present application, the embodiment of the present application also provides an integrated nucleic acid detection machine, such as Fig. 9 As shown, the nucleic acid detection integrated machine includes a PCR detector 20 and a nucleic acid extraction instrument 10 in any of the above embodiments, and the PCR detector 20 is arranged on a base plate 100 .

[0093] The nucleic acid detection all-in-one machine integrates a nucleic acid extractor 10 and a PCR detector 20, and can realize nucleic acid extraction and detection. At the same time, since the nucleic acid detection all-in-one machine adopts the nucleic acid extractor 10 in the above-mentioned embodiment, it also has the advantages and benefits brought by the above-mentioned nucleic acid extractor 10, thereby ensuring the detection accuracy and efficiency of nucleic acid extraction and detection.

[0094] The technical features of the above embodiments may be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0095] The above embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the application. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the present application shall be subject to the attached claims.

Claims

1. A nucleic acid extraction instrument, characterized in that: It comprises a base plate (100) and a sample carrier (200), a consumable carrier (300), a magnetic suction module (500), a liquid transfer module (800) and a waste liquid removal module (900) arranged on the base plate (100); The sample carrier (200) is used to place the sample tube (30); The consumable carrier (300) is used to place prepackaged consumables (40), wherein the prepackaged consumables (40) include a consumable body and a reaction chamber (41) and a reagent chamber arranged on the consumable body, wherein the reagent chamber contains a sample processing reagent; The magnetic attraction module (500) is located below the consumables carrier (300), and the magnetic attraction module (500) has a magnetic attraction component (520) that can be close to or away from the reaction chamber (41); The liquid transfer module (800) is used to transfer liquid between the sample carrier (200) and the consumable carrier (300); The waste liquid removal module (900) includes a liquid suction line (920) and a liquid suction head arranged on the liquid suction line (920), wherein the liquid suction line (920) is used to connect to a waste liquid container, and the liquid suction head can move relative to the consumable material carrier (300) and can suck the waste liquid in the reaction chamber (41) into the waste liquid container through negative pressure.

2. The nucleic acid extractor according to claim 1, characterized in that: The nucleic acid extraction instrument (10) further comprises a carrier base (400), wherein the carrier base (400) is slidably disposed on the bottom plate (100), and the sample carrier (200), the consumable carrier (300) and the magnetic suction module (500) are all disposed on the carrier base (400).

3. The nucleic acid extractor according to claim 1, characterized in that: The magnetic attraction module (500) further comprises a driving mechanism (510) and a heating body (530), wherein the magnetic attraction component (520) and the heating body (530) are both arranged on the driving mechanism (510), and the magnetic attraction component (520) and the heating body (530) can approach or move away from the reaction chamber (41) under the action of the driving mechanism (510).

4. The nucleic acid extractor according to claim 3, characterized in that: The magnetic attraction member (520) and the heating body (530) are arranged side by side, and a plurality of the pre-packaged consumables (40) can be placed side by side on the consumable carrier (300); a plurality of magnetic attraction grooves (521) are arranged at intervals on the magnetic attraction member (520), and a plurality of heating grooves (531) are arranged at intervals on the heating body (530); the plurality of pre-packaged consumables (41), the plurality of magnetic attraction grooves (521) and the plurality of heating grooves (531) correspond to each other one by one in the arrangement direction of the magnetic attraction member (520) and the heating body (530); The magnetic attraction member (520) and the heating body (530) can move in the arrangement direction of the magnetic attraction member (520) and the heating body (530) and in the height direction of the reaction chamber (41) under the action of the driving mechanism (510).

5. The nucleic acid extractor according to claim 1, characterized in that: A support frame (600) and a driving assembly (700) are arranged on the base plate (100); the support frame (600) is slidably arranged on the base plate (100) along a first direction; the driving assembly (700) is connected to the support frame (600) and is used to drive the support frame (600) to move in the first direction; the pipetting module (800) and the waste liquid removal module (900) are both arranged on the support frame (600); and the pipetting module (800) and the waste liquid removal module (900) can respectively be opposite to the sample carrier (200) and the consumable carrier (300) in the height direction of the support frame (600) during the process of moving along the first direction with the support frame (600).

6. The nucleic acid extractor according to claim 5, characterized in that: The driving assembly (700) is arranged on a side of the base plate (100) away from the support frame (600), and a driving end of the driving assembly (700) is connected to an adapter (750), and the adapter (750) is connected to an end of the support frame (600) close to the base plate (100).

7. The nucleic acid extraction instrument according to claim 5, characterized in that: The pipetting module (800) includes a first linear module (810) and a multi-channel pipetting assembly (820), wherein the first linear module (810) is arranged on the support frame (600), and the multi-channel pipetting assembly (820) is arranged on the first linear module (810) and can move in the height direction of the support frame (600) under the action of the first linear module (810), and the multi-channel pipetting assembly (820) has a plurality of mounting heads (821) for detachably mounting gun tips, and the plurality of mounting heads (821) are arranged in sequence in a second direction, and the multi-channel pipetting assembly (820) can provide each gun tip with a force to absorb or spit out a solution, and the second direction intersects with the first direction and the height direction of the support frame (600).

8. The nucleic acid extractor according to claim 5, characterized in that: The waste liquid removal module (900) also includes a second linear module (910), which is arranged on the support frame (600). The liquid suction pipeline (920) is arranged on the second linear module (910) and can move in the height direction of the support frame (600) under the action of the second linear module (910). The liquid suction pipeline (920) has a plurality of joints (921) arranged in sequence in the second direction, and each of the joints (921) is detachably connected to a liquid suction head, and the liquid suction head includes a gun head. The second direction intersects with the first direction and the height direction of the support frame (600).

9. The nucleic acid extractor according to claim 7 or 8, characterized in that: The consumables carrier (300) also has a gun tip placement position for placing a gun tip (50).

10. A nucleic acid detection integrated machine, characterized in that: It comprises a PCR detector (20) and a nucleic acid extraction instrument (10) as claimed in any one of claims 1 to 9, wherein the PCR detector (20) is arranged on the bottom plate (100).

Images