Microorganism culture dish

Abstract

The invention relates to the technical field of microbiological devices, in particular to a microbiological culture dish which comprises a dish body, a dish cover and a top cover which are arranged from bottom to top, a horn-shaped guide cover is arranged in the dish cover, the large end of the guide cover is located on the lower side, the outer edge of the guide cover is attached to the inner side wall of the dish cover, and a vent hole is coaxially formed in the small end of the guide cover; according to the culture dish, the trumpet-shaped guide cover is arranged on the upper side of the dish body, the vent holes are formed in the upper end of the guide cover, water vapor generated during microorganism culture in the dish body reaches the outside of the dish body under the drainage effect of the guide cover to be condensed, the situation that the water vapor is condensed into water drops and then drips into the dish body again is avoided, and in the microorganism culture process, the water drops are not prone to fall off. Nutrients or other substances can be added by inserting a dropper or other utensils into the dish body from the vent holes, so that infectious microbes in the air are prevented from entering the dish body, and the accuracy of a microorganism culture result is ensured.

Description

Technical Field

[0001] The invention relates to the technical field of microbiological devices, in particular to a microbiological culture dish. Background Art

[0002] Microbial culture dishes are commonly used tools in laboratories for culturing and observing microorganisms. They are mainly composed of a dish body and a lid. The dish body is usually round with a flat bottom to facilitate the uniform growth of microorganisms. The lid is usually designed to be slightly larger than the dish body, and the outer wall of the lid is provided with a downward extending edge so that the lid can firmly cover the dish body to prevent external bacteria or impurities from contaminating the culture.

[0003] During the microbial cultivation process, the evaporation of water in the culture medium and the respiration of the microorganisms will produce water vapor. After the water vapor fills the dish body, due to the temperature difference between the inside and outside of the lid, the water vapor in the dish body is easily condensed into water droplets on the inside of the lid. If the water droplets condensed on the inner wall of the lid are not cleaned in time, it is easy to cause the water droplets condensed on the inner wall of the lid to fall into the culture medium, which will cause different water contents in different areas of the culture medium, resulting in deviations in the results of microbial cultivation, or even too high water content, causing the growth rate of the microorganisms to decrease or even die.

[0004] In view of the above problems, the prior art provides some solutions. For example, the operator can regularly wipe the water droplets condensed on the inner wall of the lid, but this solution requires the lid to be opened, which easily causes the miscellaneous bacteria in the atmosphere to enter the dish body, affecting the results of microbial culture; for example, the invention patent with patent application number CN201910528511.3 provides a microbial culture dish. The technical solution provided by the invention is that by setting the inner wall of the top of the dish cover as an inclined surface, the condensed water is guided to the side wall of the dish cover through the inclined surface and then drips, and then by setting a convex block limit on the inner wall of the dish cover, the condensed water does not contact the dish body, but directly drips outside the culture dish, preventing the condensed water from flowing back, thereby ensuring the experimental effect; for example, the invention patent with patent application number CN201610401882.1 provides a conical microbial culture dish. The technical solution provided by the invention is that by setting the top of the dish cover into a cone, the conical dish cover allows the condensed liquid droplets to remain on the outside of the dish body along the conical inner wall of the dish cover, which can prevent the condensed liquid from dripping directly into the dish body. Although the above two technical solutions can solve the problem of condensation on the dish cover not flowing back into the dish body, when culture medium or other substances need to be added to the culture medium during microbial cultivation, the dish cover needs to be opened, which can easily cause bacteria in the atmosphere to enter the dish body, thereby affecting the results of microbial cultivation. Summary of the invention

[0005] The object of the present invention is to provide a microbial culture dish to solve the problem that when a conventional microbial culture dish is used to culture microorganisms, water vapor in the dish body is easily condensed into water droplets on the inner side of the lid, which easily causes the water droplets condensed on the inner wall of the lid to fall into the culture medium, thereby causing different water contents in different areas of the culture medium, resulting in deviations in the results of microbial culture, or even excessive water content, causing the growth rate of the microorganisms to decrease or even die, while solving the deficiencies of existing solutions.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A microbial culture dish comprises a dish body, a dish cover and a top cover which are arranged from bottom to top, a trumpet-shaped guide cover is arranged in the dish cover, the large end of the guide cover is located at the lower side, and the outer edge of the guide cover is in contact with the inner wall of the dish cover, a vent hole is coaxially arranged on the small end of the guide cover, the inner diameters of the dish cover and the vent hole are R1 and R2 respectively, wherein 0.2≤R2 / R1≤0.25, a sealing surface and a condensation surface are arranged at the lower end of the top cover, the condensation surface is located at the upper side of the sealing surface, and the height difference between the condensation surface and the sealing surface is 3-10 mm, the diameter of the sealing surface is R3, wherein R2<R3, and the sealing surface is coaxial with the vent hole, a limit assembly is arranged in the dish cover, and the limit assembly is used to limit the depth of the top cover inserted into the dish cover.

[0008] By arranging a trumpet-shaped guide cover on the upper side of the dish body, water vapor generated when microorganisms are cultured in the dish body reaches the vent under the drainage action of the guide cover, and is then discharged from the vent. The water vapor entering between the top cover and the guide cover fills the space between the guide cover and the top cover. Since there is a height difference between the condensation surface and the sealing surface, the distance from the condensation surface to the upper end of the top cover is smaller than the distance from the sealing surface to the condensation surface, that is, the wall thickness of the condensation surface is thinner than the wall thickness of the sealing surface, and the speed at which the temperature difference between the upper and lower sides of the condensation surface changes is faster than the speed at which the temperature difference between the upper and lower ends of the sealing surface occurs. Therefore, the water vapor between the top cover and the guide cover first condenses into water droplets on the condensation surface. Because the water vapor under the condensation surface area condenses into water droplets first, the gas pressure under the condensation surface area decreases, thereby prompting the water vapor to flow to the condensation surface, avoiding the water vapor from condensing into water droplets on the sealing surface and dripping back into the dish body, thereby ensuring the accuracy of the microorganism culture results.

[0009] Furthermore, since a vent hole is provided at the upper end of the guide cover, when it is necessary to add nutrient solution or other substances into the dish body during the microbial cultivation process, it is only necessary to separate the top cover from the dish cover, and insert a dropper or other vessel into the dish body through the vent hole to add nutrients or other substances. The diameter of the vent hole is smaller than the diameter of the dish body, thereby reducing the entry of miscellaneous bacteria in the air into the dish body and ensuring the accuracy of the microbial cultivation results.

[0010] Furthermore, when culturing aerobic microorganisms and anaerobic microorganisms, due to the different culture conditions of the two types of microorganisms, the depth of the top cover inserted into the dish cover is limited by setting a limit component, and different culture environments can be set for the two types of microorganisms. For example, when culturing aerobic microorganisms, since aerobic microorganisms need to breathe, air holes need to be opened, and when culturing anaerobic microorganisms, since oxygen will affect the growth of anaerobic microorganisms, the sealing surface is fitted with the upper end surface of the air hole through the limit component to close the air hole, thereby ensuring the accuracy of the microbial culture results.

[0011] Preferably, a plurality of limit blocks are provided on the outer wall of the dish body, and the plurality of limit blocks are distributed in a circular array along the central axis of the dish body. An annular primary sealing groove is provided on the inner wall of the dish cover, and the primary sealing groove is located on the lower side of the guide cover. The plurality of limit blocks and the inner wall of the dish cover are interference fit, and the interference range is 0.25mm to 0.5mm. The diameter of the outer wall of the dish body is R4, wherein 1.0mm≤R1-R4≤6.0mm, and the center distance from the primary sealing groove to the lower end of the guide cover is greater than the center distance from the limit block to the upper end surface of the dish body.

[0012] Since the water droplets condensed on the condensation surface directly drip onto the upper end surface of the guide hood, it is easy to cause a temperature difference between the upper and lower sides of the guide hood, thereby causing the water vapor at the lower end of the guide hood to condense on the inner wall of the guide hood; even if the water vapor at the lower end of the guide hood condenses on the inner wall of the guide hood, since the inner wall of the guide hood is a conical surface, the water droplets condensed on the inner wall of the guide hood slide on the conical surface to the inner wall of the dish cover. If the dish cover and the dish body are tightly matched, the water droplets sliding onto the dish cover can flow into the dish body along the inner wall of the dish cover. To this end, by setting the outer diameter of the dish body and the inner diameter of the dish cover to 1.0mm≤R1-R4≤6.0mm, and providing a limit block on the dish body, and providing a primary sealing groove matched with the limit block on the inner wall of the dish cover, the water droplets sliding onto the dish cover are discharged out of the dish cover through the gap between the dish cover and the dish body, thereby avoiding the water droplets condensed at the lower end of the guide hood from falling into the dish body, thereby ensuring the accuracy of the microbial culture results.

[0013] Preferably, the limit assembly includes a positioning block and a buckle, at least three positioning blocks are arranged in the dish cover, and the plurality of positioning blocks are distributed in a circular array along the central axis of the dish cover, and the plurality of positioning blocks are all located on the upper side of the guide cover, the inner wall of the positioning block is respectively provided with a guide bevel, a primary positioning groove and a secondary positioning groove from top to bottom, the buckle is arranged on the condensing surface, and the number of the buckles is the same as the number of the positioning blocks, and the plurality of buckles extend to the lower side of the condensing surface, and a hook ear is provided on the outer wall of the lower end of the buckle, and the maximum rotation outer diameter of the plurality of buckles is equal to the minimum rotation inner diameter of the plurality of positioning blocks, and an annular sealing platform is provided on the guide cover, the sealing platform is coaxial with the vent, and the inner wall diameter of the sealing platform is the same as the diameter of the vent, the diameter of the sealing surface is larger than the outer diameter of the sealing platform, and when the hook ear is inserted into the secondary positioning groove, the sealing surface fits with the upper end surface of the sealing platform.

[0014] When aerobic microorganisms are cultivated, the top cover is pressed down until the hook ear portion is embedded in the primary positioning groove. There is a gap between the sealing surface and the upper end surface of the sealing platform. The water vapor generated in the dish body can enter the space between the guide cover and the top cover through the vent hole and condense, thereby preventing the gas pressure and humidity in the dish body from being too high and affecting the growth rate of the microorganisms, thereby ensuring the accuracy of the microbial cultivation results.

[0015] Furthermore, since the gas inside the dish is relatively large and has a high pressure, the gas outside the dish cannot enter the dish through the gap between the dish and the dish cover, thereby preventing bacteria in the atmosphere from entering the dish, reducing the number of bacteria in the dish, and ensuring the accuracy of the microbial culture results.

[0016] Preferably, the positioning block and the buckle are both arc-shaped, and the central angles corresponding to the positioning block and the buckle are α1 and α2 respectively; a height limiting protrusion is provided at the lower end of the positioning block, and one side wall of the height limiting protrusion is flush with the left side wall or the rear side wall of the positioning block; the upper end of the height limiting protrusion extends to the lower end of the primary positioning groove, and the inner diameter of the height limiting protrusion is less than or equal to the inner diameter of the buckle; the central angle corresponding to the height limiting protrusion is α3, wherein (α1) / 3≤α2≤α3≤(α1) / 2; the central angle corresponding to the guide bevel is equal to the central angle of the height limiting protrusion, and the guide bevel passes through a side wall of the positioning block where the height limiting protrusion is provided.

[0017] Since the top cover needs to be pressed down when it is matched with the dish cover, and there is an interference fit between the hook ear and the positioning block, the operator needs to provide sufficient downward pressure to press the hook ear down into the primary positioning groove. However, when the downward pressure is too large, the hook ear is easily pressed directly into the secondary positioning groove, and re-operation is required. Of course, the operator can also choose to set the buckle to a position where no positioning block is set, insert the top cover into the dish cover, and then rotate the top cover to transfer the hook ear into the primary positioning groove. This operation method has high positioning requirements and is not easy to operate. For this reason, a height limiting protrusion is set at the lower end of the positioning block. When closing, it is only necessary to align the hook ear portion with the guiding bevel and press down the top cover. The buckle is elastically deformed by the inward lateral force, and the hook ear portion and the guiding bevel portion slide relatively until the hook ear portion is completely separated from the guiding bevel. The buckle loses the effect of the lateral force and resets. The hook ear portion swings with the lower end of the buckle and embeds into the primary positioning groove. Even if the downward pressure is too large, after the hook ear portion enters the primary positioning groove, the upper end surface of the height limiting boss abuts against the lower end surface of the buckle, and the height limiting component limits the buckle from continuing to move downward, thereby realizing the height limit of the top cover at the primary positioning groove, which is convenient for the operator to operate. When it is necessary to switch to the height of the secondary positioning groove, rotate the top cover until the buckle is disengaged from the height limiting protrusion, and then press down the top cover. The buckle is elastically deformed by the inward lateral force, and the hook ear part slides relative to the inner wall of the primary positioning groove until the hook ear part is completely disengaged from the primary positioning groove. The buckle loses the effect of the lateral force and resets, and the hook ear part swings with the lower end of the buckle and is embedded in the secondary positioning groove. At this time, the sealing surface is in contact with the upper end surface of the sealing platform, and the top cover cannot be pressed down any further.

[0018] Preferably, a horizontal limiting protrusion is provided on the side of the primary positioning groove away from the height limiting protrusion, the inner diameter of the horizontal limiting protrusion is equal to the outer diameter of the buckle, and the central angle corresponding to the horizontal limiting protrusion is α4, wherein α1-α3-α4<α2, the horizontal limiting protrusion extends to a side wall of the positioning block where the height limiting protrusion is not provided, a flange is provided on the upper end of the outer side wall of the top cover, and a pattern is provided on the outer side wall of the flange, and when the sealing surface is in contact with the upper end surface of the sealing platform, there is a spacing between the lower end surface of the flange and the upper end surface of the dish cover.

[0019] When transferring the hook ear part from the primary positioning groove to the secondary positioning groove, it is necessary to rotate the top cover. Since both ends of the primary positioning groove are in an open state, when the rotation angle is too large, it is easy for the hook ear part to directly fall out of the primary limit groove. For this reason, a horizontal limit block is provided in the primary positioning groove to avoid the hook ear part falling off from the primary limit groove due to excessive rotation angle, thereby facilitating the operation of the operator.

[0020] Preferably, an annular sealing ring is provided on the outer wall of the dish body, and the sealing ring is located on the lower side of the multiple limit blocks. An annular secondary sealing groove is provided on the inner wall of the dish cover, and the secondary sealing groove is located between the primary sealing groove and the guide cover. The maximum diameter of the secondary sealing groove is the same as the maximum rotational outer diameter of the multiple limit blocks, the center distance from the limit block to the sealing ring is the same as the center distance from the primary sealing groove to the secondary sealing groove, and the center distance from the primary sealing groove to the bottom of the dish cover is smaller than the center distance from the limit block to the sealing ring.

[0021] When anaerobic organisms are cultured, the top cover is pressed down until the hook ear portion is embedded in the secondary positioning groove, the sealing surface is fitted with the upper end surface of the sealing platform, and the gas between the guide cover and the top cover cannot enter the dish body through the vent hole. Since the anaerobic organisms in the dish body will first consume the gas in the dish body, the gas in the dish body is in a low-pressure state. In order to prevent the gas in the atmosphere from entering the dish body through the gap between the dish body and the dish cover, a sealing ring is provided on the outer wall of the dish body, and a secondary sealing groove is provided on the inner wall of the dish cover. When the sealing ring cooperates with the primary sealing groove, the limit block cooperates with the secondary sealing groove. The sealing ring and the primary sealing groove isolate the dish body from the atmosphere, thereby preventing the gas in the atmosphere from entering the dish body through the gap between the dish body and the dish cover, thereby ensuring the accuracy of the microbial culture results.

[0022] Furthermore, water droplets condensed on the inner wall of the guide cover flow into the gap between the dish body and the dish cover, and the sealing ring and the primary sealing groove prevent the water droplets from flowing out of the gap between the dish body and the dish cover, thereby enhancing the sealing effect between the dish body and the dish cover. Even if gas in the atmosphere enters through the matching defect between the sealing ring and the primary sealing groove, the condensed water between the dish body and the dish cover can filter the gas, thereby reducing the entry of miscellaneous bacteria into the dish body and ensuring the accuracy of the microbial culture results.

[0023] Preferably, a receiving groove is coaxially provided on the upper end surface of the top cover, the wall thickness of the top cover is in the range of 1 to 2 mm, a sealing plate is coaxially provided on the upper end of the receiving groove, the sealing plate is fixedly connected to the top cover and closes the receiving groove, and at least 2 / 3 of the volume of the cooling liquid is provided in the receiving groove.

[0024] Since the water vapor generated in the dish body condenses on the condensation surface, in order to accelerate the condensation rate of the water vapor and avoid the residence time of the water vapor on the lower side of the sealing surface, a receiving groove is provided on the top cover and a coolant is provided in the receiving groove. The heating rate of the condensation surface is reduced and the heat exchange efficiency of the condensation surface is improved, thereby increasing the condensation rate of the condensation surface to the water vapor, increasing the air pressure difference between the condensation surface and the sealing surface, and preventing the water vapor on the sealing surface from condensing and dripping into the dish body, thereby ensuring the accuracy of the microbial culture results.

[0025] Preferably, a mounting groove is coaxially opened on the upper end surface of the sealing platform, a sealing ring is arranged in the mounting groove, the sealing ring is made of elastic material, and the sealing ring extends at least 0.2 mm to the upper end surface of the sealing platform under no pressure conditions.

[0026] Furthermore, if the vent needs to be directly closed when the top cover and the dish cover are matched, at this time, it is only necessary to set the buckle to a position where the positioning block is not set, insert the top cover into the dish cover, and rotate the top cover until the sealing surface is in contact with the upper end surface of the sealing platform, so as to transfer the hook ear part into the secondary positioning groove. This operation method is more convenient, but the friction area between the sealing surface and the sealing platform is large, which may easily cause wear of the sealing surface and the upper end surface of the sealing platform, thereby reducing the sealing between the sealing platform and the sealing surface. When the hook ear part is disengaged from the secondary positioning groove, friction may also occur between the sealing surface and the sealing platform, which may easily reduce the sealing between the sealing platform and the sealing surface. For this reason, a sealing ring is set at the upper end of the sealing platform, and the sealing ring extends to the upper end surface of the sealing platform, thereby ensuring the sealing between the sealing platform and the sealing surface.

[0027] Preferably, the dish cover is made of any material including polycarbonate, polyethylene or glass, and a layer of polytetrafluoroethylene coating is sprayed on the inner wall of the guide cover. The above arrangement facilitates the cleaning and disinfection of the dish cover, avoids the adhesion of bacteria on the dish cover, and causes the dish cover to be discarded. The dish cover can be reused, thereby increasing the service life of the dish cover and reducing the cost of microbial cultivation.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] 1. The present invention provides a trumpet-shaped guide cover on the upper side of the dish body, and provides an air vent at the upper end of the guide cover. The water vapor generated during the microbial culture in the dish body reaches the outside of the dish body for condensation under the drainage effect of the guide cover, thereby preventing the water vapor from condensing into water droplets and then dripping back into the dish body. In the process of microbial culture, nutrients or other substances can be added by inserting a dropper or other utensils into the dish body through the air vent, thereby reducing the entry of miscellaneous bacteria in the air into the dish body and ensuring the accuracy of the microbial culture results.

[0030] 2. The present invention arranges a top cover on the upper side of the guide hood, arranges a condensation surface and a sealing surface with a height difference at the lower end of the top cover, and arranges a coolant in the top cover, thereby reducing the heating rate of the condensation surface and improving the heat exchange efficiency of the condensation surface, thereby increasing the condensation rate of the condensation surface to water vapor, increasing the air pressure difference between the condensation surface and the sealing surface, avoiding the water vapor on the sealing surface from condensing and dripping into the dish body, and ensuring the accuracy of the microbial culture results.

[0031] 3. The present invention sets a positioning block in the dish cover and a buckle at the lower end of the top cover to limit the depth of the top cover inserted into the dish cover. The vent holes can be opened when aerobic microorganisms are cultured, and the vent holes can be closed when anaerobic microorganisms are cultured, thereby providing different cultivation environments for the two types of microorganisms and ensuring the accuracy of the microbial culture results.

[0032] 4. The present invention provides a limit block and a sealing ring on the side wall of the dish body, and provides a primary sealing groove and a secondary sealing groove on the inner wall of the dish cover. When the primary sealing groove cooperates with the limit block, condensed water generated by condensation on the inner wall of the guide cover is discharged out of the dish cover through the gap between the dish cover and the dish body, thereby preventing water droplets condensed at the lower end of the guide cover from dripping into the dish body. When the sealing ring cooperates with the primary sealing groove, the dish body is isolated from the atmosphere, thereby preventing gases in the atmosphere from entering the dish body through the gap between the dish body and the dish cover, thereby ensuring the accuracy of the microbial culture results. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 It is a schematic diagram of the structure of the microbial culture dish of the present invention;

[0034] Figure 2 An exploded view of the microbial culture dish of the present invention;

[0035] Figure 3 A top view of the microbial culture dish of the present invention;

[0036] Figure 4 for Figure 3 Full section view at AA in the middle;

[0037] Figure 5 for Figure 4 A magnified view of point B in FIG.

[0038] Figure 6 is a full cross-sectional view of the microbial culture dish of the present invention in the second state;

[0039] Figure 7 is a full cross-sectional view of the microorganism culture dish of the present invention in the third state;

[0040] Figure 8 is a full cross-sectional view of the microbial culture dish of the present invention in a fourth state;

[0041] Fig. 9 It is a schematic diagram of the overall structure of the microorganism culture dish of the present invention in the fifth state.

[0042] In the figure: 1. dish body; 101. limit block; 102. sealing ring; 2. dish cover; 201. guide cover; 202. vent hole; 203. sealing platform; 2031. mounting groove; 204. primary sealing groove; 205. secondary sealing groove; 3. positioning block; 301. guide bevel; 302. primary positioning groove; 303. secondary positioning groove; 304. height limit protrusion; 305. horizontal limit protrusion; 4. top cover; 401. sealing surface; 402. condensation surface; 403. receiving groove; 404. buckle; 4041. hook ear; 405. flange; 5. sealing plate; 6. culture medium; 7. cooling liquid; S1. pressing direction; R, rotation direction. DETAILED DESCRIPTION

[0043] See also Figures 1 to 9 The present invention provides a microbial culture dish, and the technical solution is as follows:

[0044] See also Figures 1 to 5 A microbial culture dish, comprising a dish body 1, a dish cover 2 and a top cover 4 arranged from bottom to top, a trumpet-shaped guide cover 201 is arranged in the dish cover 2, the dish cover 2 is made of a polycarbonate material, and a layer of polytetrafluoroethylene coating is sprayed on the inner wall of the guide cover 201, the large end of the guide cover 201 is located at the lower side, and the outer edge of the guide cover 201 is in contact with the inner wall of the dish cover 2, and a vent hole 202 is coaxially arranged on the small end of the guide cover 201, the inner diameter of the dish cover 2 is R1=160.0mm, the inner diameter of the vent hole 202 is R2=35.0mm, and the top cover A sealing surface 401 and a condensing surface 402 are provided at the lower end of the top cover 4, the height difference between the sealing surface 401 and the condensing surface 402 is 5.0 mm, and the condensing surface 402 is located on the upper side of the sealing surface 401, the diameter of the sealing surface 401 is R3=45.0 mm, and the sealing surface 401 is coaxial with the vent hole 202; a receiving groove 403 is coaxially opened on the upper end surface of the top cover 4, the wall thickness of the top cover 4 is 2.5 mm, and a sealing plate 5 is coaxially provided on the upper end of the receiving groove 403, the sealing plate 5 is fixedly connected to the top cover 4, and closes the receiving groove 403, and the receiving groove 403 is filled with coolant 7.

[0045] The outer wall diameter of the dish body 1 is R4=155.0mm, the inner diameter of the dish body 1 is 150.0mm, and the bottom of the dish body 1 is provided with a culture medium 6; a plurality of stop blocks 101 are provided on the outer wall of the dish body 1, and the plurality of stop blocks 101 are distributed in a circular array along the central axis of the dish body 1, and an annular primary sealing groove 204 is provided on the inner wall of the dish cover 2, and the primary sealing groove 204 is located on the lower side of the guide cover 201, and the plurality of stop blocks 101 and the inner wall of the dish cover 2 are interference fit, and the interference amount is 0.3mm, and the center distance from the primary sealing groove 204 to the lower end of the guide cover 201 is greater than the center distance from the stop block 101 to the upper end surface of the dish body 1, and the dish body 1 An annular sealing ring 102 is provided on the outer wall, and the sealing ring 102 is located at the lower side of the plurality of limit blocks 101. An annular secondary sealing groove 205 is provided on the inner wall of the dish cover 2, and the secondary sealing groove 205 is located between the primary sealing groove 204 and the guide cover 201. The maximum diameter of the secondary sealing groove 205 is the same as the maximum rotation outer diameter of the plurality of limit blocks 101. The center distance from the limit block 101 to the sealing ring 102 is the same as the center distance from the primary sealing groove 204 to the secondary sealing groove 205, and the center distance from the primary sealing groove 204 to the bottom of the dish cover 2 is 4.0 mm, and the center distance from the limit block 101 to the sealing ring 102 is 5.0 mm.

[0046] See also Figures 3 to 5 , Fig. 9Three positioning blocks 3 are arranged in the dish cover 2, and the three positioning blocks 3 are arranged in a circular array along the central axis of the dish cover 2, and the three positioning blocks 3 are all located on the upper side of the guide cover 201, and the inner side wall of the positioning block 3 is respectively provided with a guide bevel 301, a primary positioning groove 302 and a secondary positioning groove 303 from top to bottom, and the buckles 404 are arranged on the condensation surface 402, and the number of the buckles 404 is the same as the number of the positioning blocks 3, and the three buckles 404 extend to the lower side of the condensation surface 402, and the outer side wall of the lower end of the buckle 404 is provided with a hook ear portion 4041, and the maximum rotation outer diameter of the three buckles 404 is equal to the minimum rotation inner diameter of the plurality of positioning blocks 3, and the guide cover 201 is provided with an annular sealing platform 2 03, the sealing platform 203 is coaxial with the vent hole 202, and the inner wall diameter of the sealing platform 203 is the same as the diameter of the vent hole 202, the outer diameter of the sealing platform 203 is 40.0 mm, when the hook ear portion 4041 is inserted into the primary positioning groove 302, the distance between the sealing surface 401 and the upper end surface of the sealing platform 203 is 5.0 mm, when the hook ear portion 4041 is inserted into the secondary positioning groove 303, the sealing surface 401 fits with the upper end surface of the sealing platform 203; a mounting groove 2031 is coaxially opened on the upper end surface of the sealing platform 203, and a sealing ring is arranged in the mounting groove 2031, the sealing ring is made of elastic material, and the sealing ring extends to 0.2 mm on the upper end surface of the sealing platform 203 under no pressure conditions. mm; the positioning block 3 and the buckle 404 are both arc-shaped, and the central angle α1 corresponding to the positioning block 3 is 30°, and the central angle corresponding to the buckle 404 is α2=12°. A height limiting protrusion 304 is provided at the lower end of the positioning block 3, and one side wall of the height limiting protrusion 304 is flush with the left side wall or the rear side wall of the positioning block 3. The upper end of the height limiting protrusion 304 extends to the lower end of the primary positioning groove 302, and the inner diameter of the height limiting protrusion 304 is less than or equal to the inner diameter of the buckle 404. The central angle corresponding to the height limiting protrusion 304 is α3=12.5°, and the central angle corresponding to the guide bevel 301 is equal to the central angle of the height limiting protrusion 304, and the guide The bevel 301 passes through a side wall of the positioning block 3 on which a height limiting protrusion 304 is provided; a horizontal limiting protrusion 305 is provided on the side of the primary positioning groove 302 away from the height limiting protrusion 304, the inner diameter of the horizontal limiting protrusion 305 is equal to the outer diameter of the buckle 404, and the central angle corresponding to the horizontal limiting protrusion 305 is α4=5°, the horizontal limiting protrusion 305 extends to a side wall of the positioning block 3 on which no height limiting protrusion 304 is provided, a flange 405 is provided at the upper end of the outer side wall of the top cover 4, and a pattern is provided on the outer side wall of the flange 405, and when the sealing surface 401 is in contact with the upper end surface of the sealing platform 203, there is at least 3.0 mm between the lower end surface of the flange 405 and the upper end surface of the dish cover 2.

[0047] When culturing aerobic microorganisms, refer to Figures 1 to 9, set the culture medium 6 in the dish body 1, sterilize the prepared culture medium 6 and other experimental materials, and after the sterilization is completed, inoculate the target strain into the culture medium 6 under aseptic operation; coaxially sleeve the dish cover 2 onto the dish body 1, see Figure 4 and Figure 5It should be noted that, for aerobic microorganism cultivation, it is only necessary to embed multiple limit blocks 101 into the primary sealing groove 204; then the top cover 4 is inserted into the dish cover 2 from top to bottom. During insertion, the multiple buckles 404 are respectively aligned with the multiple guide bevels 301, and then a downward pressing force is applied to the top cover 4. At this time, during the pressing process, the buckle 404 is elastically deformed by the inward lateral force, and the hook ear portion 4041 slides relative to the guide bevel 301 until the hook ear portion 4041 is completely separated from the guide bevel 301, and the buckle 404 loses the effect of the lateral force and The hook ear 4041 swings along with the lower end of the buckle 404 and is embedded in the primary positioning groove 302. If the downward pressure applied to the top cover 4 has not been removed at this time, the buckle 404 continues to move downward in the primary positioning groove 302 until the lower end of the buckle 404 abuts against the height limiting protrusion 304, and the downward movement of the top cover 4 is hindered. When the pressure continues to be pressed, the position of the top cover 4 cannot continue to move downward, and the downward pressure applied to the top cover 4 is removed, thereby completing the assembly of the aerobic microbial culture dish. During the aerobic microbial culture process, the culture medium 6 and the aerobic microbial respiration produce The water vapor first fills the dish body 1, and then diffuses to the lower side of the guide cover 201. During the drainage process of the guide cover 201, the water vapor flows upward along the lower end surface of the guide cover 201 to the vent, and then passes through the vent hole 202 to reach the upper end of the guide cover 201. After the water vapor contacts the sealing surface 401, it diffuses to the outside of the sealing surface 401 until the water vapor contacts the condensation surface 402. Due to the setting of the coolant 7, the water vapor condenses after contacting the condensation surface 402 and forms water droplets that fall to the upper side of the guide cover 201. Since the water vapor on the condensation surface 402 condenses As a result, the gas pressure in the area of ​​the condensation surface 402 decreases, thereby promoting the water vapor in the area on the blocking surface to flow to the condensation surface 402; further, since the water droplets condensed on the condensation surface 402 fall onto the upper end surface of the guide cover 201, the water vapor on the lower end surface of the guide cover 201 will condense, but since the lower end surface of the guide cover 201 is a conical surface and a layer of polytetrafluoroethylene is sprayed on the inner wall of the guide cover 201, the water droplets condensed on the lower end surface of the guide cover 201 flow to the inner wall of the dish cover 2, and are discharged from the dish body 1 through the gap between the dish cover 2 and the dish body 1. When it is necessary to add nutrient solution or other substances to the culture medium 6, the top cover 4 is rotated in the opposite direction of the R direction, and the hook ear portion 4041 slides in the primary positioning groove 302 until the hook ear portion 4041 slides out of the primary positioning groove 302. The top cover 4 can be separated from the dish cover 2, and the nutrient solution or other substances can be added to the dish body 1 through a test tube or other experimental equipment. After the addition is completed, it is only necessary to repeat the above operation of matching the top cover 4 with the dish cover 2, which will not be repeated here.

[0048] When culturing anaerobic microorganisms, refer to Figures 1 to 9, a culture medium 6 is set in the dish body 1, and the prepared culture medium 6 and other experimental materials are sterilized. After the sterilization is completed, the target strain is inoculated into the culture medium 6 under aseptic operation; the dish cover 2 is coaxially sleeved on the dish body 1. It should be noted that, unlike the aerobic microbial culture, for the anaerobic microbial culture, multiple limit blocks 101 need to be embedded in the secondary sealing groove 205, and the sealing ring 102 needs to be embedded in the primary sealing groove 204, that is, the lower end of the dish body 1 and the dish cover 2 are in a fully sealed state; then the top cover 4 is inserted into the dish cover 2 from top to bottom, please refer to Figure 4 When the top cover 4 is in the state of being pressed downward, the buckle 404 is elastically deformed by the inward lateral force, and the hook ear portion 4041 slides relatively with the guiding bevel 301 until the hook ear portion 4041 completely disengages from the guiding bevel 301, and the buckle 404 loses the effect of the lateral force and is reset. The hook ear portion 4041 swings with the lower end of the buckle 404 and is embedded in the primary positioning groove 302. Under the action of the downward pressure, the lower end of the buckle 404 abuts against the height limiting protrusion 304, and the downward movement of the top cover 4 is hindered. The position of the top cover 4 cannot continue to move downward, and the downward pressure applied to the top cover 4 is removed, and the top cover 4 is rotated along the R direction until the side wall of the hook ear portion 4041 abuts against the horizontal limiting protrusion 305, and the horizontal rotation of the top cover 4 is restricted. The state of the top cover 4 is changed from Figure 4 The state changes to Figure 6 For status, see Figure 6 , press the top cover 4 along the S direction, at this time, the lower end of the buckle 404 is separated from the height limiting protrusion 304, and the top cover 4 moves downward under the action of the downward pressure. The buckle 404 is elastically deformed by the inward lateral force, and the hook ear part 4041 slides relative to the inner wall of the primary positioning groove 302 until the hook ear part 4041 is completely separated from the primary positioning groove 302. The buckle 404 loses the effect of the lateral force and resets. The hook ear part 4041 swings with the lower end of the buckle 404 and embeds into the secondary positioning groove 303. At this time, the sealing surface 401 is in contact with the upper end surface of the sealing platform 203, the vent hole 202 is blocked, and the space between the dish body 1 and the guide cover 201 is completely closed. Please refer to Figure 7 After the anaerobic microorganisms are cultured, the top cover 4 is rotated in the R direction, and the hook portion 4041 slides in the secondary positioning groove 303 until the hook portion 4041 slides out of the secondary positioning groove 303. The state of the top cover 4 is changed from Figure 7 The state changes to Figure 8 The top cover 4 can be separated from the dish cover 2, and the dish body 1 can be pulled out from the lower end of the dish cover 2.

[0049] The above two specific embodiments of the present invention are described in detail in conjunction with the accompanying drawings, but the present invention is not limited to the embodiments described above. For those skilled in the art, various changes, modifications, substitutions and variations of these embodiments without departing from the principles and ideas of the present invention should still fall within the scope of protection of the present invention.

Claims

1. A microbial culture dish, characterized in that: The invention comprises a dish body (1), a dish cover (2) and a top cover (4) arranged from bottom to top, wherein a trumpet-shaped guide cover (201) is arranged inside the dish cover (2), the large end of the guide cover (201) is located at the bottom, and the outer edge of the guide cover (201) is in contact with the inner wall of the dish cover (2), and a vent hole (202) is coaxially arranged on the small end of the guide cover (201), and the inner diameters of the dish cover (2) and the vent hole (202) are R1 and R2 respectively, wherein 0.2≤R2 / R1≤0.25, and the top cover A sealing surface (401) and a condensation surface (402) are provided at the lower end of the dish cover (4), the condensation surface (402) is located on the upper side of the sealing surface (401), and the height difference between the condensation surface (402) and the sealing surface (401) is 3 to 10 mm, the diameter of the sealing surface (401) is R3, wherein R2<R3, and the sealing surface (401) is coaxial with the vent hole (202), and a limit assembly is provided in the dish cover (2), and the limit assembly is used to limit the depth of the top cover (4) inserted into the dish cover (2).

2. A microbial culture dish according to claim 1, characterized in that: A plurality of limit blocks (101) are arranged on the outer wall of the dish body (1), and the plurality of limit blocks (101) are arranged in a circular array along the central axis of the dish body (1). An annular primary sealing groove (204) is provided on the inner wall of the dish cover (2), and the primary sealing groove (204) is located at the lower side of the guide cover (201). The plurality of limit blocks (101) and the inner wall of the dish cover (2) are interference fit, and the interference range is 0.25 mm to 0.5 mm. The diameter of the outer wall of the dish body (1) is R4, wherein 1.0 mm ≤ R1-R4 ≤ 6.0 mm. The center distance from the primary sealing groove (204) to the lower end of the guide cover (201) is greater than the center distance from the limit block (101) to the upper end surface of the dish body (1).

3. A microbial culture dish according to claim 2, characterized in that: The limiting assembly comprises a positioning block (3) and a buckle (404); at least three positioning blocks (3) are arranged in the dish cover (2); the plurality of positioning blocks (3) are arranged in a circular array along the central axis of the dish cover (2); and the plurality of positioning blocks (3) are all located on the upper side of the guide cover (201); the inner side wall of the positioning block (3) is respectively provided with a guiding bevel (301), a primary positioning groove (302) and a secondary positioning groove (303) from top to bottom; the buckle (404) is arranged on the condensing surface (402); and the number of the buckles (404) is the same as the number of the positioning blocks (3); and the plurality of buckles (404) are all extended toward the lower side of the condensing surface (402). The outer wall of the lower end of the buckle (404) is provided with a hook ear portion (4041); the maximum rotation outer diameter of the plurality of buckles (404) is equal to the minimum rotation inner diameter of the plurality of positioning blocks (3); the guide cover (201) is provided with an annular sealing platform (203); the sealing platform (203) is coaxial with the vent hole (202); the inner wall diameter of the sealing platform (203) is the same as the diameter of the vent hole (202); the diameter of the sealing surface (401) is greater than the outer diameter of the sealing platform (203); when the hook ear portion (4041) is inserted into the secondary positioning groove (303), the sealing surface (401) is in contact with the upper end surface of the sealing platform (203).

4. A microbial culture dish according to claim 3, characterized in that: The positioning block (3) and the buckle (404) are both arc-shaped, and the central angles corresponding to the positioning block (3) and the buckle (404) are α1 and α2 respectively. A height limiting protrusion (304) is provided at the lower end of the positioning block (3). A side wall of the height limiting protrusion (304) is flush with the left side wall or the rear side wall of the positioning block (3). The upper end of the height limiting protrusion (304) extends to the lower end of the primary positioning groove (302). The inner diameter of the limiting protrusion (304) is less than or equal to the inner diameter of the buckle (404), the central angle corresponding to the height limiting protrusion (304) is α3, wherein (α1) / 3≤α2≤α3≤(α1) / 2, the central angle corresponding to the guiding bevel (301) is equal to the central angle of the height limiting protrusion (304), and the guiding bevel (301) passes through a side wall of the positioning block (3) on which the height limiting protrusion (304) is provided.

5. A microbial culture dish according to claim 4, characterized in that: A horizontal limiting protrusion (305) is provided on one side of the primary positioning groove (302) away from the height limiting protrusion (304); the inner diameter of the horizontal limiting protrusion (305) is equal to the outer diameter of the buckle (404); the central angle corresponding to the horizontal limiting protrusion (305) is α4, wherein α1-α3-α4<α2; the horizontal limiting protrusion (305) extends to a side wall of the positioning block (3) where the height limiting protrusion (304) is not provided; a flange (405) is provided at the upper end of the outer wall of the top cover (4); the outer wall of the flange (405) is provided with a pattern; when the sealing surface (401) is in contact with the upper end surface of the sealing platform (203), there is a gap between the lower end surface of the flange (405) and the upper end surface of the dish cover (2).

6. A microbial culture dish according to claim 2, characterized in that: An annular sealing ring (102) is provided on the outer side wall of the dish body (1), and the sealing ring (102) is located at the lower side of the plurality of limit blocks (101). An annular secondary sealing groove (205) is provided on the inner side wall of the dish cover (2), and the secondary sealing groove (205) is located between the primary sealing groove (204) and the guide cover (201). The maximum diameter of the secondary sealing groove (205) is the same as the maximum rotation outer diameter of the plurality of limit blocks (101). The center distance from the limit block (101) to the sealing ring (102) is the same as the center distance from the primary sealing groove (204) to the secondary sealing groove (205), and the center distance from the primary sealing groove (204) to the bottom of the dish cover (2) is smaller than the center distance from the limit block (101) to the sealing ring (102).

7. A microbial culture dish according to claim 1, characterized in that: A receiving groove (403) is coaxially provided on the upper end surface of the top cover (4), the wall thickness of the top cover (4) is in the range of 1 to 3 mm, a sealing plate (5) is coaxially provided on the upper end of the receiving groove (403), the sealing plate (5) is fixedly connected to the top cover (4) and closes the receiving groove (403), and at least 2 / 3 of the volume of the cooling liquid (7) is provided in the receiving groove (403).

8. A microbial culture dish according to claim 3, characterized in that: A mounting groove (2031) is coaxially formed on the upper end surface of the sealing platform (203), a sealing ring is arranged in the mounting groove (2031), the sealing ring is made of an elastic material, and the sealing ring extends at least 0.2 mm above the upper end surface of the sealing platform (203) under no pressure conditions.

9. A microbial culture dish according to claim 1, characterized in that: The dish cover (2) is made of any one of polycarbonate, polyethylene or glass, and a layer of polytetrafluoroethylene coating is sprayed on the inner wall of the guide cover (201).

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