Quantitative inoculation equipment for pathogenic bacteria of corn ear rot

Abstract

The invention discloses quantitative inoculation equipment for pathogenic bacteria of corn ear rot, which relates to the technical field of agricultural disease prevention and control inoculation and comprises a body, a box body is fixedly connected to one side of the body, an operation panel is fixedly mounted on the other side of the body, a barrel is fixedly connected and communicated with one side, far away from the body, of the box body, and an inoculation needle is arranged in the box body. The inoculation needle extends into the barrel and is in sliding connection with the barrel, the inoculation needle is in transmission connection with an ejection assembly, the ejection assembly is fixedly connected with the inner wall of the box body, an adjusting assembly is installed on the side wall of the box body and used for adjusting the insertion depth of the inoculation needle, a liquid storage barrel is fixedly installed on the top face of the body, and a metering pump is installed in the body. The metering pump is communicated with the liquid storage barrel and the inoculation needle through pipelines, a driving box is arranged below the box body, a clamping assembly is installed in the driving box, and the clamping assembly extends out of the driving box and makes contact with corncobs. The insertion depth of the inoculating needle can be adjusted, and adaptive adjustment can be carried out according to different conditions.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural crop disease control and inoculation, and in particular to a quantitative inoculation device for pathogenic bacteria of corn ear rot. Background Art

[0002] Corn ear rot, also known as corn kernel rot, is caused by the infection of more than 20 kinds of fungi such as Fusarium graminearum, Fusarium moniliforme, Trichoderma, Penicillium, Aspergillus, and Cladosporium, which cause the corn ears or kernels to rot. Corn is the most widely planted crop in the world and plays an important role in ensuring food and feed security and alleviating the energy crisis. With the vigorous promotion of returning straw to the field, high-density planting and the extensive use of nitrogen fertilizers, the accumulation of pathogens in the soil continues to increase, resulting in the increasingly serious occurrence of corn ear rot. At present, there is no effective method for preventing and controlling corn ear rot through pesticides in production. Breeding and promoting disease-resistant varieties are the most effective technical measures to solve the harm of ear rot. By inoculating corn breeding materials or varieties with pathogens, their resistance to ear rot can be identified and evaluated, which is an important technical measure for the cultivation and promotion of disease-resistant varieties.

[0003] At present, there is no special equipment for quantitative inoculation of corn ear rot pathogens in China. Most researchers use a combination of medical syringes and electric drills to complete the inoculation work.

[0004] Therefore, there is an urgent need for a quantitative inoculation device for corn ear rot pathogens to solve the problem of corn ear rot pathogen inoculation. Summary of the invention

[0005] The purpose of the invention is to provide a quantitative inoculation device for corn ear rot pathogens to solve the problems existing in the prior art.

[0006] To achieve the above-mentioned purpose, the present invention provides the following scheme: The present invention provides a quantitative inoculation device for corn ear rot pathogens, comprising a main body, a box body fixedly connected to one side of the main body, an operation panel fixedly installed on the other side of the main body, a side of the box body away from the main body fixedly connected to and connected with a cylinder body, an inoculation needle is provided in the box body, the inoculation needle extends into the cylinder body and is slidably connected to the cylinder body, the inoculation needle is transmission-connected with an ejection assembly, the ejection assembly is fixed to the inner wall of the box body, an adjustment assembly is installed on the side wall of the box body, the adjustment assembly is used to adjust the insertion depth of the inoculation needle, a liquid storage barrel is fixedly installed on the top surface of the main body, a metering pump is installed in the main body, the metering pump is respectively connected to the liquid storage barrel and the inoculation needle through pipelines, a driving box is provided under the box body, a clamping assembly is installed in the driving box, the clamping assembly extends out of the driving box and contacts with the corn ear.

[0007] Preferably, the ejection assembly includes a baffle symmetrically fixed to the inner wall of the box body, a first spring is fixed to the side wall of the baffle, one end of the first spring away from the baffle is fixed to a limit plate, and the limit plate is fixedly sleeved on the inoculation needle.

[0008] Preferably, a connecting plate is symmetrically fixed to the inner wall of the box body, an electromagnet is fixed to the side wall of the connecting plate, the electromagnet is magnetically connected to the limit plate, the electromagnet is electrically connected to the operation panel, and the first spring passes through the connecting plate.

[0009] Preferably, the adjustment assembly includes a protective shell fixedly connected to the side wall of the box body, a screw is rotatably connected inside the protective shell, a slider is threadedly connected to the screw, the slider is slidably connected to the inner wall of the protective shell, one end of the screw extends out of the protective shell and is fixedly connected to a knob, an adjustment plate is fixedly connected to the side wall of the slider, the adjustment plate extends into the box body and is slidably connected to the inner wall of the box body, the inoculation needle passes through the adjustment plate and is slidably connected to the adjustment plate, and the adjustment plate is used to limit the position of the limit plate.

[0010] Preferably, a first hydraulic rod is symmetrically fixed to the inner wall of the box body, and the first hydraulic rod is used to reset the limit plate. Through holes are symmetrically opened on the adjustment plate, and the diameter of the through holes is larger than the maximum diameter of the first hydraulic rod.

[0011] Preferably, a connecting cylinder is fixedly connected to a side of the inner wall of the box body away from the first hydraulic rod, the end of the inoculation needle extends into the connecting cylinder and is slidably connected to the connecting cylinder, and the connecting cylinder is connected to the metering pump through a pipeline.

[0012] Preferably, a slide groove is provided on the top surface of the protective shell, an indicator plate is slidably connected in the slide groove, the indicator plate is fixedly connected to the slider, and scales are written on the top surface of the protective shell to facilitate adjustment of the insertion depth of the inoculation needle.

[0013] Preferably, the clamping assembly includes a symmetrically arranged long rod, the long rod extends out of the drive box and is fixedly connected to a clamping plate, the clamping plate is in contact with the corn cob, one end of a second spring is fixedly connected to the side wall of the long rod, the other end of the second spring is fixedly connected to the inner wall of the drive box, an extension plate is fixedly connected to the side of the long rod away from the second spring, a cylinder is fixedly connected to the top surface of the extension plate, a conical head is in contact between the two cylinders, the conical head is fixedly connected to the output end of the second hydraulic rod, and the second hydraulic rod is fixedly connected to the inner wall of the drive box.

[0014] Preferably, a connecting rod is fixedly connected to the bottom surface of the box body, the bottom of the connecting rod is fixedly connected to the driving box, reinforcement rods are symmetrically provided on both sides of the connecting rod, and both ends of the reinforcement rod are respectively fixedly connected to the box body and the driving box.

[0015] Preferably, a handle is fixedly connected to a side of the top surface of the main body away from the liquid storage barrel, and an armrest is fixedly connected to a side of the bottom surface of the main body close to the operation panel.

[0016] The present invention discloses the following technical effects: when in use, the corn cob is clamped and fixed by a clamping assembly, the depth of the inoculation needle to be inserted is adjusted by an adjusting assembly, and then the inoculation needle is ejected from the cylinder by an ejection assembly and inserted into the corn cob, the amount of suspension to be injected is input through an operation panel, the metering pump is started, and the suspension in the liquid storage barrel is passed into the inoculation needle to complete the inoculation of the corn cob. The present invention can adjust the insertion depth of the inoculation needle, can be adaptively adjusted according to different situations, and can fix the corn cob by a clamping assembly to ensure the stability of the inoculation process, without the need for multiple people to cooperate, and can be operated by one person. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The drawings constituting a part of the present application are used to provide a further understanding of the present application. The illustrative embodiments and descriptions of the present application are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0018] Figure 1 It is a structural schematic diagram of the present invention;

[0019] Figure 2 It is a front view schematic diagram of the present invention;

[0020] Figure 3 It is a schematic diagram of the internal structure of the box body of the present invention;

[0021] Figure 4 It is a schematic diagram of the top view of the box body of the present invention;

[0022] Figure 5 This is a schematic diagram of the internal structure of the protective shell of the present invention;

[0023] Figure 6 It is a schematic diagram of a top view of a drive box of the present invention;

[0024] In the figure: 1. main body; 2. liquid storage barrel; 3. handle; 4. operation panel; 5. armrest; 6. box body; 7. cylinder body; 8. splint; 9. drive box; 10. long rod; 11. connecting rod; 12. inoculation needle; 13. first hydraulic rod; 14. adjustment plate; 15. limit plate; 16. electromagnet; 17. first spring; 18. baffle; 19. connecting cylinder; 20. protective shell; 21. lead screw; 22. slider; 23. knob; 24. slide; 25. indicator plate; 26. second spring; 27. extension plate; 28. cylinder; 29. ​​second hydraulic rod; 30. conical head. DETAILED DESCRIPTION

[0025] Corn ear rot is an important disease that affects corn yield and quality. In order to screen corn germplasm resources resistant to these diseases, it is necessary to accurately inoculate and identify the pathogens of corn ears. However, there is currently no special equipment for quantitative inoculation of corn ear rot pathogens in China.

[0026] Equipment Overview: The quantitative inoculation equipment for corn ear rot pathogens belongs to the field of plant pathogen inoculation technology. By accurately controlling the inoculation dosage and inoculation position, the quantitative inoculation of corn ears is achieved, thereby improving the accuracy and reliability of inoculation identification. The main structure of the equipment includes a support box, a splint, a liquid storage bottle, an inoculation box, a rubber piston, a needle and related connecting parts.

[0027] Main structure and function: The support box is the main part of the whole equipment, which is used to fix and support other parts. Two clamps are symmetrically and slidably arranged at the bottom of the support box for clamping corn ears. A liquid storage bottle is fixed on the top of the support box for storing ear rot bacteria suspension or stem rot bacteria spore suspension. The clamp is used to clamp the corn ear to prevent the corn from shifting during inoculation. The clamps are symmetrically arranged at the bottom of the support box and can slide along the slide. Two sliders are symmetrically fixed on the top of each clamp, and the sliders are arranged inside the slide. One of the clamps is fixed at one end of the slide, and the other clamp can be clamped and released by the rebound action of the spring. An arc groove is opened on the side wall of the clamp, which fits the shape of the corn better and prevents the corn from deformation. The liquid storage bottle is fixed on the top of the support box for storing the pathogen suspension. The bottom side wall of the liquid storage bottle is connected to one end of the inoculation box through an infusion tube to transport the pathogen suspension into the inoculation box. The liquid storage bottle is provided with a water injection hole and a pressure valve. The water injection hole is used to add the pathogen suspension into the liquid storage bottle. The pressure valve is used to connect the electric pressurizing device. The pressure in the bottle is maintained constant by pumping gas into the liquid storage bottle. The inoculation box is arranged inside the support box and is used to store and transport the pathogen suspension. A rubber piston is arranged inside the inoculation box. The rubber piston is in sealing contact with the inner wall of the inoculation box and forms a liquid storage cavity with the inner wall of the inoculation box. A piston rod is fixed on the top of the rubber piston, and a scale line is printed on the piston rod, which is used to control the volume of the pathogen suspension inhaled into the inoculation box. The rubber piston is arranged inside the inoculation box and is in sealing contact with the inner wall of the inoculation box. A piston rod is fixed on the top of the rubber piston, and a scale line is printed on the piston rod. By pushing or pulling the piston rod, the up and down movement of the rubber piston can be controlled, thereby realizing the inhalation and discharge of the pathogen suspension. The needle is fixed at the bottom of the inoculation box and is used to puncture and inject the pathogen suspension into the corn cob. The needle is connected to the inside of the inoculation box through a straight tube, and a check valve is arranged inside the straight tube to prevent the pathogen suspension from flowing back. The number and spacing of the needles can be adjusted as needed to achieve multi-point inoculation. The infusion tube connects the liquid storage bottle and the inoculation box, and is used to transport the pathogen suspension from the liquid storage bottle to the inoculation box. The infusion tube passes through the support box and the inoculation box and extends to the inside of the liquid storage cavity. A check valve is arranged inside one end of the infusion tube located in the inoculation box to prevent the pathogen suspension from flowing back into the liquid storage bottle. The annular push rod is fixed to the top of the inoculation box to push the inoculation box up and down. An annular push plate is fixed on the annular push rod to facilitate the staff to push. The annular push rod and the piston rod can move up and down in the annular hole and the through hole opened on the support box respectively, so as to realize the stable movement of the inoculation box. The check valve is arranged inside the infusion tube and the straight tube to prevent the pathogen suspension from flowing back. The check valve includes a bent baffle and two protrusions. One end of the bent baffle is rotatably mounted on one of the protrusions through a rotating shaft, and the other end is mounted on the other protrusion. When the pathogen suspension flows in from the infusion tube or the straight tube, the bent baffle is pushed open; when the pathogen suspension tries to flow back, the bent baffle blocks the channel to prevent backflow.The limit plate is symmetrically arranged on the inner wall of the support box, and is located above the inoculation box. The limit plate is used to prevent the height of the inoculation box from being pulled too high, so that the needle cannot completely pass through the needle hole during inoculation. A cylindrical plate is fixed on the top of the piston rod, and an annular push plate is fixed on the top of the annular push rod. The design of the cylindrical plate and the annular push plate makes it easy for the staff to push the rubber piston and the inoculation box.

[0028] Working principle and operation process: The needle hole and the annular hole opened on the support box respectively limit the needle and the annular push rod. The annular push rod drives the inoculation box to move stably, so that when the same corn is inoculated, the two acupuncture needle bundles can be inserted into the same position, while preventing the needle from bending. The rubber piston is in sealed contact with the inner wall of the inoculation box to form a liquid storage chamber. The suction and discharge of the pathogen suspension can be controlled by pushing or pulling the piston rod. The setting of the check valve prevents the pathogen suspension from flowing back. (1) Preparation: Add the prepared pathogen suspension into the liquid storage bottle and seal the water injection hole. Place the corn ear in the arc groove between the two clamps, loosen the clamps, and under the rebound action of the spring, the two clamps clamp the corn to the bottom of the support box and face the needle. (2) Inhalation of pathogen suspension: Observe the scale line on the piston rod according to the concentration of the suspension, pull the cylindrical plate upward to form a negative pressure in the liquid storage chamber, and then inhale the most appropriate amount of pathogen suspension from the infusion tube into the inoculation box. (3) Inoculation: Push the annular push plate to push the inoculation box to the bottom of the support box, so that multiple needles are inserted into the corn cob. Push the cylindrical plate again to inoculate the pathogen suspension in the liquid storage chamber into the inside of the corn cob. Pull out the annular push plate to separate the needles from the corn cob. (4) Repeat inoculation: Pull the cylindrical plate to continue to inhale the same amount of pathogen suspension as the last time. Repeat the above steps to inoculate the pathogen suspension in the liquid storage chamber into the same position and depth of the corn again, and inoculate the same corn with the pathogen suspension twice. (5) Release the corn: After the inoculation is completed, pull the annular push plate to separate the needles from the corn cob. Then pull one of the clamps to release the corn. Repeat the above steps to perform secondary acupuncture inoculation on different corns.

[0029] Equipment advantages: An inoculation box with a maximum capacity of 500ml of pathogen suspension is set. According to the concentration of the suspension, the scale line on the piston rod is observed to control the volume of the suspension in the inoculation box to be sucked in, so as to accurately control the most appropriate amount of pathogens for the same corn to be inoculated twice. This makes the inoculation amount of the suspension most conducive to the comparison of disease resistance research and identification test results, and also makes the inoculation identification screening results faster. The needle hole and the annular hole opened on the support box play a role in limiting the needle bundle and the annular push rod respectively. The annular push rod drives the inoculation box to move stably, so that when the same corn is inoculated, the two acupuncture needle bundles can be inserted into the same position, while preventing the needle from bending. This improves the accuracy of the inoculation process, reduces manual errors, and is conducive to the smooth progress of inoculation identification. Two symmetrically arranged splints clamp the inoculated corn, effectively preventing the corn from shifting during inoculation. This ensures that the two acupuncture positions are the same, and the design of the slider and spring makes the clamping process simpler and more convenient. Pulling one of the splints can achieve the clamping and loosening of the corn. The device is easy to operate and the vaccination process can be completed by pushing and pulling the piston rod, annular push plate and other components. At the same time, the design of the device allows multiple needles to be vaccinated at the same time, improving the vaccination efficiency.

[0030] Although the equipment has certain advantages in quantitative inoculation of corn ear rot pathogens, it still has some shortcomings and needs further improvement and optimization. At present, the operation of the equipment still needs to be completed manually, including steps such as clamping corn, pushing the piston rod, and pulling the annular push plate. In order to improve the efficiency and accuracy of inoculation, it is possible to consider introducing automated control technology, such as using stepper motors, cylinders and other driving components to achieve automated operation. During the inoculation process, the needle may be blocked by impurities or sediments in the pathogen suspension. To solve this problem, a filtering device, such as a microporous filter, can be set at the front end of the needle to filter out impurities and sediments in the suspension. At present, the equipment controls the inoculation depth by manually pushing the annular push plate, but the inoculation depth is not easy to control accurately. To solve this problem, a depth adjustment device, such as a screw micrometer, can be set on the inoculation box to accurately control the inoculation depth. The inoculation box, infusion tube, needle and other components need to be cleaned and maintained after use, but the current structure of the equipment makes cleaning and maintenance inconvenient. To solve this problem, a detachable component structure can be designed, such as using quick connectors, clamps and other connecting components for easy disassembly and cleaning.

[0031] The quantitative inoculation equipment for corn ear rot pathogens is mainly suitable for places such as plant pathology laboratories, agricultural research institutions, and corn breeding units. In plant pathology laboratories, the equipment can be used to study the pathogenesis of corn ear rot and stem rot, the mode of pathogen transmission, and disease prevention and control measures. In agricultural research institutions and corn breeding units, the equipment can be used to screen germplasm resources resistant to corn ear rot and stem rot, and provide technical support for the cultivation of disease-resistant varieties. Through precise control, quantitative inoculation of pathogen suspension can be achieved, avoiding problems such as uneven inoculation dose and inaccurate inoculation position in traditional inoculation methods. This not only improves the inoculation accuracy, but also makes the inoculation identification results more reliable. The use of multiple needles for simultaneous inoculation greatly improves the inoculation efficiency. At the same time, the equipment has a high degree of automation, which reduces the time and labor cost of manual operation. Through inoculation identification, germplasm resources resistant to corn ear rot and stem rot can be screened. These disease-resistant varieties can effectively resist the invasion of diseases during the planting process, reduce the use of pesticides, and improve the yield and quality of corn. The application provides strong technical support for plant pathology research and corn breeding. By using this equipment for scientific research, we can gain a deeper understanding of the pathogenesis and prevention and control measures of corn ear rot, providing a scientific basis for agricultural production.

[0032] In order to ensure the normal operation of the equipment and extend its service life, the equipment needs to be regularly maintained and serviced. Cleaning equipment: After each use, the various parts of the equipment should be cleaned in time, especially the parts that are easily contaminated by germs, such as the infusion tube, needle and inoculation box. Special cleaning agents and tools should be used for cleaning, and corrosive chemical reagents should be avoided. Checking parts: Regularly check whether the various parts of the equipment are intact, such as whether the infusion tube is aging, whether the needle is worn, etc. If damaged or worn parts are found, they should be replaced or repaired in time. Lubricating parts: For parts that need lubrication, such as piston rods and annular push plates, an appropriate amount of lubricating oil should be applied regularly to reduce friction and wear. Calibration equipment: Calibrate the equipment regularly to ensure its precision and accuracy. Special calibration tools and methods should be used for calibration, and the instruction manual of the equipment should be followed. Storing equipment: When the equipment is not in use, it should be stored in a dry, ventilated environment without corrosive gases, avoiding direct sunlight and high temperature. At the same time, the various parts of the equipment should be disassembled and stored separately to avoid mutual squeezing and damage.

[0033] With the advancement of science and technology and the development of agricultural production, the quantitative inoculation equipment for corn ear rot pathogens is also constantly developing and improving. Intelligence: By introducing advanced technologies such as the Internet of Things, big data, and artificial intelligence, the intelligent control and remote monitoring of the equipment can be realized. Users can use terminal devices such as mobile phones or computers to understand the operating status of the equipment, inoculation dosage, inoculation location and other information in real time, thereby improving the efficiency and accuracy of inoculation. Automation: Further improve the automation level of the equipment and reduce the time and labor cost of manual operation. For example, automatic control systems and robotics technology can be introduced to realize functions such as automatic clamping, automatic inoculation and automatic cleaning of the equipment. Precision: By optimizing the structure and design of the equipment, more accurate inoculation control can be achieved. For example, precise transmission mechanisms and positioning systems can be introduced to improve the accuracy and stability of inoculation. At the same time, more intelligent inoculation algorithms and strategies can be developed to realize personalized inoculation plans based on the growth of corn and the propagation characteristics of pathogens. Diversification: Develop equipment with multiple inoculation modes and functions for different types of corn ear rot. For example, replaceable needles and infusion tubes can be designed to meet the inoculation needs of different pathogens. At the same time, a software platform with multiple data processing and analysis functions can be developed to provide users with more comprehensive vaccination identification services.

[0034] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0036] Reference Figure 1-Figure 6 As shown, the present embodiment provides a quantitative inoculation device for corn ear rot pathogens, including a main body 1, a box body 6 is fixedly connected to one side of the main body 1, an operation panel 4 is fixedly installed on the other side of the main body 1, a side of the box body 6 away from the main body 1 is fixedly connected and connected to a cylinder 7, an inoculation needle 12 is provided in the box body 6, the inoculation needle 12 extends into the cylinder 7 and is slidably connected to the cylinder 7, the inoculation needle 12 is transmission-connected with an ejection assembly, the ejection assembly is fixedly connected to the inner wall of the box body 6, an adjustment assembly is installed on the side wall of the box body 6, the adjustment assembly is used to adjust the insertion depth of the inoculation needle 12, a liquid storage barrel 2 is fixedly installed on the top surface of the main body 1, a metering pump is installed in the main body 1, the metering pump is connected to the liquid storage barrel 2 and the inoculation needle 12 through pipelines, a driving box 9 is provided below the box body 6, a clamping assembly is installed in the driving box 9, the clamping assembly extends out of the driving box 9 and contacts with the corn ear.

[0037] When in use, the corn cob is clamped and fixed by the clamping assembly, the depth of the inoculation needle 12 to be inserted is adjusted by the adjusting assembly, and then the inoculation needle 12 is ejected from the barrel 7 by the ejection assembly and inserted into the corn cob, and the amount of suspension to be injected is input through the operation panel 4, and the metering pump is started to pass the suspension in the liquid storage barrel 2 into the inoculation needle 12 to complete the inoculation of the corn cob. The present invention can adjust the insertion depth of the inoculation needle 12, can be adaptively adjusted according to different situations, and can fix the corn cob by the clamping assembly to ensure the stability of the inoculation process, without the need for multiple people to cooperate, and can be operated by one person.

[0038] In a further optimized solution, the ejection assembly includes a baffle 18 symmetrically fixed to the inner wall of the box body 6, a first spring 17 is fixed to the side wall of the baffle 18, and one end of the first spring 17 away from the baffle 18 is fixed to the limit plate 15, and the limit plate 15 is fixedly sleeved on the inoculation needle 12. The limit plate 15 is ejected by the rebound of the first spring 17, and the limit plate 15 drives the inoculation needle 12 to move, and then the inoculation needle 12 is inserted into the corn ear.

[0039] In a further optimized solution, a connecting plate is symmetrically fixed to the inner wall of the box body 6, an electromagnet 16 is fixed to the side wall of the connecting plate, the electromagnet 16 is magnetically connected to the limit plate 15, the electromagnet 16 is electrically connected to the operation panel 4, and the first spring 17 passes through the connecting plate. When the electromagnet 16 is powered on, the limit plate 15 is sucked, and the electromagnet 16 is powered off by the operation panel 4. At this time, the compressed first spring 17 rebounds, and the limit plate 15 and the inoculation needle 12 are ejected together, which is convenient for inoculation.

[0040] Further optimized solution, the adjustment component includes a protective shell 20 fixed to the side wall of the box body 6, a lead screw 21 is rotatably connected in the protective shell 20, a slider 22 is threadedly connected to the lead screw 21, the slider 22 is slidably connected to the inner wall of the protective shell 20, one end of the lead screw 21 extends out of the protective shell 20 and is fixed with a knob 23, an adjustment plate 14 is fixed to the side wall of the slider 22, the adjustment plate 14 extends into the box body 6 and is slidably connected to the inner wall of the box body 6, the inoculation needle 12 passes through the adjustment plate 14 and is slidably connected to the adjustment plate 14, and the adjustment plate 14 is used to limit the position of the limit plate 15. Turning the knob 23 drives the lead screw 21 to rotate, the lead screw 21 drives the slider 22 to move in the protective shell 20, the slider 22 drives the adjustment plate 14 to move, and the distance between the adjustment plate 14 and the limit plate 15 is the insertion depth of the inoculation needle 12, which is convenient for adjusting the insertion depth.

[0041] In a further optimized solution, the inner wall of the box body 6 is symmetrically fixed with a first hydraulic rod 13, which is used to reset the limit plate 15. The adjustment plate 14 is symmetrically provided with through holes, and the diameter of the through holes is larger than the maximum diameter of the first hydraulic rod 13. When the inoculation is completed, the first hydraulic rod 13 extends until it contacts the limit plate 15 and pushes the limit plate 15 to move, until the limit plate 15 moves to the electromagnet 16 and attracts the electromagnet 16, and the first hydraulic rod 13 is retracted to facilitate the next inoculation.

[0042] In a further optimized solution, a connecting cylinder 19 is fixedly connected to the inner wall of the box body 6 on one side away from the first hydraulic rod 13, and the end of the inoculation needle 12 extends into the connecting cylinder 19 and is slidably connected to the connecting cylinder 19, and the connecting cylinder 19 is connected to the metering pump through a pipeline. The connecting cylinder 19 limits the inoculation needle 12 to ensure that the inoculation needle 12 slides stably and horizontally, and the suspension enters the inoculation needle 12 through the connecting cylinder 19, thereby completing the inoculation.

[0043] In a further optimized solution, a slide groove 24 is provided on the top surface of the protective shell 20, and an indicator plate 25 is slidably connected in the slide groove 24. The indicator plate 25 is fixedly connected to the slider 22, and a scale is written on the top surface of the protective shell 20 to facilitate adjustment of the insertion depth of the inoculation needle 12. When the slider 22 moves, the indicator plate 25 is driven to move, and the insertion depth of the inoculation needle 12 is determined by observing the relative position of the indicator plate 25 and the scale, which is convenient for adjustment.

[0044] A further optimized solution is that the clamping assembly includes a symmetrically arranged long rod 10, the long rod 10 extends out of the drive box 9 and is fixedly connected to a clamping plate 8, the clamping plate 8 is in contact with the corn cob, one end of the second spring 26 is fixedly connected to the side wall of the long rod 10, the other end of the second spring 26 is fixedly connected to the inner wall of the drive box 9, an extension plate 27 is fixedly connected to the side of the long rod 10 away from the second spring 26, a cylinder 28 is fixedly connected to the top surface of the extension plate 27, a conical head 30 is in contact between the two cylinders 28, the conical head 30 is fixedly connected to the output end of the second hydraulic rod 29, and the second hydraulic rod 29 is fixedly connected to the inner wall of the drive box 9. The second hydraulic rod 29 extends and drives the conical head 30 to move. The conical head 30 squeezes the cylinder 28. The cylinder 28 drives the extension plate 27 to move. The extension plate 27 drives the long rod 10 to move in the opposite direction. At this time, the two clamping plates 8 move away from each other. When the corn cob needs to be clamped, the second hydraulic rod 29 contracts, and the two long rods 10 move toward each other under the action of the second spring 26, thereby driving the two clamping plates 8 to move toward each other until the corn cob is clamped and fixed.

[0045] Further optimization scheme, the bottom surface of the box body 6 is fixedly connected with a connecting rod 11, the bottom of the connecting rod 11 is fixedly connected to the drive box 9, and reinforcing rods are symmetrically arranged on both sides of the connecting rod 11, and the two ends of the reinforcing rods are respectively fixedly connected to the box body 6 and the drive box 9. The setting of the connecting rod 11 and the reinforcing rod ensures the stability of the connection of the drive box 9.

[0046] In a further optimized solution, a handle 3 is fixedly connected to the top surface of the body 1 away from the liquid storage barrel 2, and an armrest 5 is fixedly connected to the bottom surface of the body 1 close to the operation panel 4. The handle 3 is held in one hand and the armrest 5 is held in the other hand to ensure stability during use.

[0047] Furthermore, the operation panel 4 includes a microprocessor, a display screen and operation buttons; the microprocessor is used to receive parameters input by the user and control the entire vaccination process based on these parameters, and the display screen and operation buttons are used to control various parameters and status information during the vaccination process, as well as provide a user interaction interface.

[0048] Furthermore, a pressure sensor and a pressure regulating valve are installed in the liquid storage barrel 2 to monitor and adjust the pressure during inoculation to ensure that the suspension can be inoculated evenly and deeply into the corn cob.

[0049] In the description of the present invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0050] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A quantitative inoculation device for corn ear rot pathogens, characterized in that: The invention comprises a main body (1), a box body (6) is fixedly connected to one side of the main body (1), an operation panel (4) is fixedly installed to the other side of the main body (1), a side of the box body (6) away from the main body (1) is fixedly connected to and communicated with a cylinder (7), an inoculation needle (12) is arranged in the box body (6), the inoculation needle (12) extends into the cylinder (7) and is slidably connected to the cylinder (7), the inoculation needle (12) is transmission-connected to an ejection assembly, the ejection assembly is fixedly connected to the inner wall of the box body (6), and the inoculation needle (12) is connected to the inner wall of the box body (6). An adjusting component is installed on the side wall of the box body (6), and the adjusting component is used to adjust the insertion depth of the inoculation needle (12). A liquid storage barrel (2) is fixedly installed on the top surface of the main body (1), and a metering pump is installed in the main body (1). The metering pump is connected to the liquid storage barrel (2) and the inoculation needle (12) through pipelines. A driving box (9) is provided below the box body (6), and a clamping component is installed in the driving box (9). The clamping component extends out of the driving box (9) and contacts with the corn cob.

2. The quantitative inoculation device for corn ear rot pathogen according to claim 1, characterized in that: The ejection assembly comprises a baffle (18) symmetrically fixed to the inner wall of the box body (6), a first spring (17) is fixed to the side wall of the baffle (18), one end of the first spring (17) away from the baffle (18) is fixed to a limit plate (15), and the limit plate (15) is fixedly sleeved on the inoculation needle (12).

3. The quantitative inoculation device for the pathogen of corn ear rot according to claim 2, characterized in that: A connecting plate is symmetrically fixed to the inner wall of the box body (6), an electromagnet (16) is fixed to the side wall of the connecting plate, the electromagnet (16) is magnetically connected to the limit plate (15), the electromagnet (16) is electrically connected to the operation panel (4), and the first spring (17) passes through the connecting plate.

4. The quantitative inoculation device for the pathogen of corn ear rot according to claim 2, characterized in that: The adjustment component includes a protective shell (20) fixedly connected to the side wall of the box body (6), a lead screw (21) rotatably connected inside the protective shell (20), a slider (22) threadedly connected to the lead screw (21), the slider (22) slidably connected to the inner wall of the protective shell (20), one end of the lead screw (21) extends out of the protective shell (20) and is fixedly connected to a knob (23), an adjustment plate (14) is fixedly connected to the side wall of the slider (22), the adjustment plate (14) extends into the box body (6) and is slidably connected to the inner wall of the box body (6), the inoculation needle (12) passes through the adjustment plate (14) and is slidably connected to the adjustment plate (14), and the adjustment plate (14) is used to limit the position of the limit plate (15).

5. The quantitative inoculation device for the pathogen of corn ear rot according to claim 4 is characterized in that: A first hydraulic rod (13) is symmetrically fixed to the inner wall of the box body (6), and the first hydraulic rod (13) is used to reset the limit plate (15). The adjustment plate (14) is symmetrically provided with through holes, and the diameter of the through holes is larger than the maximum diameter of the first hydraulic rod (13).

6. The quantitative inoculation device for the pathogen of corn ear rot according to claim 5, characterized in that: A connecting tube (19) is fixedly connected to the inner wall of the box body (6) on the side away from the first hydraulic rod (13), and the end of the inoculation needle (12) extends into the connecting tube (19) and is slidably connected to the connecting tube (19), and the connecting tube (19) is connected to the metering pump through a pipeline.

7. The quantitative inoculation device for the pathogen of corn ear rot according to claim 4, characterized in that: The top surface of the protective shell (20) is provided with a slide groove (24), and an indicator plate (25) is slidably connected in the slide groove (24). The indicator plate (25) is fixedly connected to the slider (22). The top surface of the protective shell (20) is marked with scales to facilitate the adjustment of the insertion depth of the inoculation needle (12).

8. The quantitative inoculation device for the pathogen of corn ear rot according to claim 1, characterized in that: The clamping assembly comprises a symmetrically arranged long rod (10), the long rod (10) extending out of the driving box (9) and fixedly connected to a clamping plate (8), the clamping plate (8) being in contact with the corn cob, one end of a second spring (26) being fixedly connected to the side wall of the long rod (10), the other end of the second spring (26) being fixedly connected to the inner wall of the driving box (9), an extension plate (27) being fixedly connected to the side of the long rod (10) away from the second spring (26), a cylinder (28) being fixedly connected to the top surface of the extension plate (27), a conical head (30) being in contact between the two cylinders (28), the conical head (30) being fixedly connected to the output end of a second hydraulic rod (29), the second hydraulic rod (29) being fixedly connected to the inner wall of the driving box (9).

9. The quantitative inoculation device for the pathogen of corn ear rot according to claim 1, characterized in that: A connecting rod (11) is fixedly connected to the bottom surface of the box body (6), and the bottom of the connecting rod (11) is fixedly connected to the driving box (9). Reinforcement rods are symmetrically provided on both sides of the connecting rod (11), and the two ends of the reinforcement rods are respectively fixedly connected to the box body (6) and the driving box (9).

10. The quantitative inoculation device for the pathogen of corn ear rot according to claim 1, characterized in that: A handle (3) is fixedly connected to the side of the top surface of the main body (1) away from the liquid storage barrel (2), and an armrest (5) is fixedly connected to the side of the bottom surface of the main body (1) close to the operating panel (4).

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